CN113233937A - Bio-organic fertilizer particle coating agent and preparation method thereof - Google Patents
Bio-organic fertilizer particle coating agent and preparation method thereof Download PDFInfo
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- CN113233937A CN113233937A CN202110703810.3A CN202110703810A CN113233937A CN 113233937 A CN113233937 A CN 113233937A CN 202110703810 A CN202110703810 A CN 202110703810A CN 113233937 A CN113233937 A CN 113233937A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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Abstract
The invention relates to the technical field of materials and fertilizers, and particularly discloses a biological organic fertilizer particle coating agent and a preparation method thereof. The pollution coating agent is prepared by taking exopolysaccharide, polyvinyl alcohol, activated carbon powder and water as raw materials and stirring, shearing and dispersing uniformly. The biological organic fertilizer particle coating agent can improve the appearance commodity of fertilizer products, so that the coated fertilizer is convenient and sanitary to use, the coating material is easy to degrade, environment-friendly and low in soil pollution, microorganisms in the organic fertilizer can survive and grow for a long time, and the biological organic fertilizer particle coating agent has a synergistic effect on the fertilizer and a function of improving plant immunity.
Description
Technical Field
The invention belongs to the technical field of materials and fertilizers, and particularly relates to a biological organic fertilizer particle coating agent and a preparation method thereof.
Background
The biological organic fertilizer not only has the characteristic of comprehensive nutrient elements, but also has the functions of improving soil, improving soil hardening and the like. Meanwhile, beneficial microorganisms in the bio-organic fertilizer enter soil to actively reproduce, so that soil pores and secretions can be effectively improved, plant growth can be stimulated, and the diversity of beneficial bacteria and soil organisms can be increased. In addition, the microorganisms entering the soil can also inhibit the propagation of harmful pathogenic bacteria, enhance the immunity of crops and reduce the risk of plant diseases and insect pests. Therefore, the bio-organic fertilizer is an environment-friendly fertilizer with complete functions. In order to enable the biological organic fertilizer to have good commercial appearance, be not easy to disintegrate in transportation and storage and be sanitary and convenient to use, the biological organic fertilizer is usually required to be coated, a plurality of coating agents and microorganisms cannot coexist, and beneficial microorganisms in the coated biological organic fertilizer cannot survive and propagate for a long time, so that the biological organic fertilizer coating agent beneficial to the survival and propagation of the microorganisms is of great significance.
Disclosure of Invention
The invention aims to provide a biological organic fertilizer particle coating agent and a preparation method thereof, the biological organic fertilizer particle coating agent can improve the appearance commodity of fertilizer products, so that the coated fertilizer is more convenient and sanitary to use, is environment-friendly, has little soil pollution, can be beneficial to the long-time survival and growth of microorganisms, and has the synergistic effect on the fertilizer and the function of improving the immunity of plants.
In order to achieve the purpose, the invention provides a bio-organic fertilizer particle coating agent, which comprises 20-25% of exopolysaccharide, 4.5-5.5% of polyvinyl alcohol, 0.5-1% of activated carbon powder and water.
Further, in the technical scheme, the extracellular polysaccharide is formed by mixing 0.9-1 part of bacillus polymyxa extracellular polysaccharide, 5-5.5 parts of bacillus mucilaginosus extracellular polysaccharide and 2.5-3 parts of bacillus amyloliquefaciens extracellular polysaccharide in parts by weight.
Further, in the technical scheme, the fermentation medium for fermenting the bacillus polymyxa exopolysaccharide comprises 60-70 g/L of sucrose, 30-40 g/L of peptone, 35-45 g/L of yeast powder, 0.5-10 g/L of monopotassium phosphate, 0.1-2 g/L of manganese sulfate, 0.2-2 g/L of ammonium nitrate, 0.05-0.1 g/L of sodium chloride and 0.05-0.1 g/L of ferric chloride.
Further, in the technical scheme, the fermentation medium for fermenting the bacillus mucilaginosus exopolysaccharide comprises 30-50 g/L of sucrose, 0.4-8 g/L of monopotassium phosphate, 0.2-20 g/L of magnesium sulfate heptahydrate, 0.2-2 g/L of ammonium nitrate, 0.05-0.1 g/L of ferric chloride and 0.03-0.05 g/L of calcium carbonate.
Further, in the technical scheme, the fermentation medium for fermenting the bacillus amyloliquefaciens exopolysaccharide comprises 20-40 g/L of maltose, 30-40 g/L of yeast powder, 0.3-6 g/L of diammonium citrate, 0.2-20 g/L of magnesium sulfate heptahydrate, 0.5-1 g/L of ammonium nitrate, 0.05-0.1 g/L of sodium chloride, 1-3 g/L of ammonium nitrate and 0.05-0.1 g/L of ferric chloride.
Further, in the technical scheme, the average polymerization degree of the polyvinyl alcohol is 1000-1800, and the alcoholysis degree is 78-88%.
Further, in the technical scheme, the particle size of the activated carbon powder is 200-300 meshes.
Further, in the technical scheme, the diameter of the aperture of the activated carbon powder is 100-200 nm.
Further, the preparation method of the bio-organic fertilizer particle coating agent provided by the invention comprises the following steps of dissolving polyvinyl alcohol in water to form a solution with the mass percentage of 8-10%, adding the rest water, extracellular polysaccharide and activated carbon powder according to the mass ratio, stirring, shearing and dispersing uniformly to obtain the bio-organic fertilizer particle coating agent.
Further, in the technical scheme, the temperature of water for dissolving the polyvinyl alcohol is 50-95 ℃, and the stirring and dissolving time is 1-4 hours.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a bio-organic fertilizer coating agent which takes exopolysaccharide, polyvinyl alcohol, activated carbon powder and water as main components and can form bright appearance particles when being used for coating a bio-organic fertilizer, so that the appearance commodity is improved, and the coating agent provides good survival and propagation conditions and humidity for microorganisms through the exopolysaccharide and the activated carbon components, thereby ensuring the long-term survival of the microorganisms in the organic fertilizer.
2. The extracellular polysaccharide component contained in the coating agent is beneficial to improving the water retention and moisture retention of soil, preventing soil hardening and improving the drought resistance of crops, and meanwhile, the extracellular polysaccharide is contained in the coating agent and is matched with active carbon with good water adsorption effect to realize a synergistic effect, so that the water retention and moisture retention capability of the soil is more outstanding, and the soil is favorably improved. On the other hand, the extracellular polysaccharide is also beneficial to improving the physiological activities of the plants such as disease resistance, stress resistance and the like, promoting the plants to absorb the nutrient components of the fertilizer and improving the fertilizer efficiency of the fertilizer.
3. Compared with the common coating agent, the coating agent of the invention takes exopolysaccharide, polyvinyl alcohol, activated carbon powder and water as main components, and has the advantages of easy degradation, little influence of degraded substances on environment, no soil pollution and the like.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
1. Preparation of extracellular polysaccharide
(1) Preparation of exopolysaccharide from exopolysaccharide microorganism of bacillus polymyxa
Fermentation reaction culture medium:
73g/L of sucrose, 34g/L of peptone, 41g/L of yeast powder, 5g/L of monopotassium phosphate, 1g/L of manganese sulfate, 1g/L of ammonium nitrate, 0.07g/L of sodium chloride, 0.09g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus polymyxa into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shake culture at 35 ℃, 200rpm for 5 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(2) Preparation of extracellular polysaccharide of bacillus mucilaginosus microbe
Fermentation reaction culture medium:
40g/L of sucrose, 6g/L of monopotassium phosphate, 15g/L of magnesium sulfate heptahydrate, 1g/L of ammonium nitrate, 0.07g/L of ferric chloride, 0.04g/L of calcium carbonate and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then carrying out sterilization treatment at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the gel-like bacillus into the fermentation reaction culture medium, wherein the inoculation amount is 5%, carrying out shaking culture at 32 ℃ and 220rpm for 4 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(3) Preparation of bacillus amyloliquefaciens exopolysaccharide
Fermentation reaction culture medium:
30g/L of maltose, 35g/L of yeast powder, 3g/L of diammonium citrate, 15g/L of magnesium sulfate heptahydrate, 0.8g/L of ammonium nitrate, 0.07g/L of sodium chloride, 2g/L of ammonium nitrate, 0.07g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus amyloliquefaciens into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shaking culture at 30 ℃, 200rpm for 7 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(4) Mixing 1 part of bacillus polymyxa exopolysaccharide, 5.3 parts of bacillus mucilaginosus exopolysaccharide and 2.7 parts of bacillus amyloliquefaciens exopolysaccharide according to the mass parts to prepare the needed exopolysaccharide for later use.
2. Preparation of bio-organic fertilizer particle coating agent
(1) The coating agent comprises the following components: 24% of exopolysaccharide, 5% of polyvinyl alcohol, 0.7% of activated carbon powder and water.
The average polymerization degree of the polyvinyl alcohol is 1600, and the alcoholysis degree is 78%; the particle size of the activated carbon powder is 200-300 meshes, and the diameter of the aperture of the activated carbon powder is 100-200 nm.
(2) Preparation method of coating agent
Dissolving polyvinyl alcohol in water at the temperature of 85 ℃, stirring and dissolving for 2 hours to form 9% solution, adding the rest water, extracellular polysaccharide and activated carbon powder according to the mass ratio, stirring, and shearing and dispersing at 1500rpm for 5 minutes by using a shearing machine to prepare the bio-organic fertilizer particle coating agent.
The prepared coating agent is used for coating the biological organic fertilizer to obtain granules with bright appearance.
3. Influence of coating agent on survival of functional microbial inoculum
In the test, the prepared coating agent is used as a test sample (sample 1), 5% polyvinyl alcohol solution (comparative sample 1-1) is used as a comparative sample, the selected test bacteria are commonly used bacillus licheniformis, and related equipment, vessels and culture media are subjected to normal sterilization treatment. The experimental method comprises the following steps: 3g of Bacillus licheniformis strain was added to 20g of the coating agent (sample 1) or polyvinyl alcohol solution (comparative sample 1-1), shaken and allowed to stand for 2 hours and 4 hours. In a laboratory fumigated by formaldehyde, an ultraviolet lamp is adopted to perform sterilization treatment on an ultra-clean workbench for more than half an hour. Coating with 2 hr or 4 hr coating agent or 5% polyvinyl alcohol solution, diluting, gradient coating, and sealing with flame, and collecting dilution gradient 107And 108And 4 times of parallel coating are carried out, the obtained product is placed into an incubator to be cultivated for 2 days at the constant temperature of 32-33 ℃ (the used culture medium is nutrient agar culture medium), the colony number is counted, and the result is shown in tables 1 and 2. As is clear from tables 1 and 2, the survival of Bacillus licheniformis was found to be favorable in sample 1, while the survival of Bacillus licheniformis was not favorable in comparative sample 1-1.
TABLE 1 dilution gradient 107Culture statistics of colony count
TABLE 2 dilution gradient 108Culture statistics of colony count
Example 2
1. Preparation of extracellular polysaccharide
(1) Preparation of exopolysaccharide from exopolysaccharide microorganism of bacillus polymyxa
Fermentation reaction culture medium:
60g/L of sucrose, 30g/L of peptone, 35g/L of yeast powder, 0.5g/L of monopotassium phosphate, 0.1g/L of manganese sulfate, 0.2g/L of ammonium nitrate, 0.05g/L of sodium chloride, 0.05g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus polymyxa into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shake culture at 35 ℃, 200rpm for 5 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(2) Preparation of extracellular polysaccharide of bacillus mucilaginosus microbe
Fermentation reaction culture medium:
30g/L of sucrose, 0.4g/L of monopotassium phosphate, 0.2g/L of magnesium sulfate heptahydrate, 0.2g/L of ammonium nitrate, 0.05g/L of ferric chloride, 0.03g/L of calcium carbonate and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then carrying out sterilization treatment at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the gel-like bacillus into the fermentation reaction culture medium, wherein the inoculation amount is 5%, carrying out shaking culture at 32 ℃ and 220rpm for 4 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(3) Preparation of bacillus amyloliquefaciens exopolysaccharide
Fermentation reaction culture medium:
20g/L of maltose, 30g/L of yeast powder, 0.3g/L of diammonium citrate, 0.2g/L of magnesium sulfate heptahydrate, 0.5g/L of ammonium nitrate, 0.05g/L of sodium chloride, 1g/L of ammonium nitrate, 0.05g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus amyloliquefaciens into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shaking culture at 30 ℃, 200rpm for 7 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(4) Mixing 0.9 part of bacillus polymyxa exopolysaccharide, 5 parts of bacillus mucilaginosus exopolysaccharide and 2.5 parts of bacillus amyloliquefaciens exopolysaccharide according to the mass parts to prepare the needed exopolysaccharide for later use.
2. Preparation of bio-organic fertilizer particle coating agent
(1) The coating agent comprises the following components: 20% of exopolysaccharide, 4.5% of polyvinyl alcohol, 0.5% of activated carbon powder and water.
The polyvinyl alcohol has an average polymerization degree of 1000 and an alcoholysis degree of 78%; the particle size of the activated carbon powder is 200-300 meshes, and the diameter of the aperture of the activated carbon powder is 100-200 nm.
(2) Preparation method of coating agent
Dissolving polyvinyl alcohol in water at the temperature of 50 ℃, stirring and dissolving for 4 hours to form 8% solution, adding the rest water, extracellular polysaccharide and activated carbon powder according to the mass ratio, stirring, and shearing and dispersing at 1500rpm for 5 minutes by using a shearing machine to prepare the bio-organic fertilizer particle coating agent.
The prepared coating agent is used for coating the biological organic fertilizer to obtain granules with bright appearance.
3. Influence of coating agent on survival of functional microbial inoculum
In the test, the prepared coating agent is used as a test sample (sample 2), 5% polyvinyl alcohol solution is used as a comparison sample (comparison sample 2-1), the selected test bacteria are commonly used bacillus subtilis, and related equipment, vessels and culture media are subjected to normal sterilization treatment. The experimental method comprises the following steps: 3g of Bacillus subtilis strain was added to 20g of the coating agent (sample 2) or polyvinyl alcohol solution (comparative sample 2-1), shaken, and allowed to stand for 2 hours and 4 hours. In a laboratory fumigated by formaldehyde, an ultraviolet lamp is adopted to perform sterilization treatment on an ultra-clean workbench for more than half an hour. Coating with 2 hr or 4 hr coating agent or 5% polyvinyl alcohol solution, diluting, gradient coating, and sealing with flame, and collecting dilution gradient 107And 108And performing parallel coating for 4 times, putting the mixture into an incubator for 2 days at the constant temperature of 32-33 ℃, wherein the used culture medium is a nutrient agar culture medium, counting the number of colonies, and obtaining the results shown in tables 3 and 4. From tables 3 and 4, it is understood that Bacillus subtilis survival was favored in sample 2, but not in comparative sample 2-1.
TABLE 3 dilution gradient 107Culture statistics of colony count
TABLE 4 dilution gradient 108Culture statistics of colony count
4. Comparison of survival rates of active bacterial colonies in different coating agent coated biological organic fertilizers
Respectively carrying out particle coating treatment on the biological organic fertilizer by using an equivalent sample 2, a comparison sample 2-1 and a comparison sample 2-2 (polyurethane resin coating agent purchased from the market), drying at low temperature, and detecting the survival rate of effective viable bacteria in different storage timeAnd (4) rate. The used biological organic fertilizer is obtained by inoculating main raw materials of pig manure, manioc waste and bagasse to bacillus subtilis strain for fermentation, and the effective viable count is 5 multiplied by 1010And the organic matter content is 51 percent. The survival rate of the effective viable count of the coated bio-organic fertilizer particles is shown in table 5, and the table shows that the survival rate of the bio-organic fertilizer coated by the sample 2 is obviously higher than that of the effective viable count in a comparative sample, and the survival time is obviously longer.
TABLE 5 survival rate of active colony number in different coating agent coated bio-organic fertilizer
Example 3
1. Preparation of extracellular polysaccharide
(1) Preparation of exopolysaccharide from exopolysaccharide microorganism of bacillus polymyxa
Fermentation reaction culture medium:
70g/L of sucrose, 40g/L of peptone, 45g/L of yeast powder, 10g/L of monopotassium phosphate, 2g/L of manganese sulfate, 2g/L of ammonium nitrate, 0.1g/L of sodium chloride, 0.1g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus polymyxa into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shake culture at 35 ℃, 200rpm for 5 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(2) Preparation of extracellular polysaccharide of bacillus mucilaginosus microbe
Fermentation reaction culture medium:
50g/L of sucrose, 8g/L of monopotassium phosphate, 20g/L of magnesium sulfate heptahydrate, 2g/L of ammonium nitrate, 0.1g/L of ferric chloride, 0.05g/L of calcium carbonate and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then carrying out sterilization treatment at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the gel-like bacillus into the fermentation reaction culture medium, wherein the inoculation amount is 5%, carrying out shaking culture at 32 ℃ and 220rpm for 4 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(3) Preparation of bacillus amyloliquefaciens exopolysaccharide
Fermentation reaction culture medium:
40g/L of maltose, 40g/L of yeast powder, 6g/L of diammonium citrate, 20g/L of magnesium sulfate heptahydrate, 1g/L of ammonium nitrate, 0.1g/L of sodium chloride, 3g/L of ammonium nitrate, 0.1g/L of ferric chloride and distilled water.
Fermentation culture
Preparing a culture medium according to the formula proportion of a fermentation reaction culture medium, then sterilizing at 115 ℃ for 20min to obtain the fermentation reaction culture medium, inoculating the bacillus amyloliquefaciens into the fermentation reaction culture medium, wherein the inoculation amount is 5%, performing shaking culture at 30 ℃, 200rpm for 7 days, and centrifuging to obtain a fermentation supernatant; dialyzing the fermented supernatant, and centrifuging to obtain dialyzed supernatant; and filtering the dialyzed supernatant, and performing vacuum freeze drying to obtain the exopolysaccharide.
(4) Mixing 1 part of bacillus polymyxa exopolysaccharide, 5.5 parts of bacillus mucilaginosus exopolysaccharide and 3 parts of bacillus amyloliquefaciens exopolysaccharide according to the mass parts to prepare the needed exopolysaccharide for later use.
2. Preparation of bio-organic fertilizer particle coating agent
(1) The coating agent comprises the following components: 25% of extracellular polysaccharide, 5.5% of polyvinyl alcohol, 1% of activated carbon powder and water. The average polymerization degree of the polyvinyl alcohol is 1800, and the alcoholysis degree is 88%; the particle size of the activated carbon powder is 200-300 meshes, and the diameter of the aperture of the activated carbon powder is 100-200 nm.
(2) Preparation method of coating agent
Dissolving polyvinyl alcohol in water at the temperature of 95 ℃, stirring and dissolving for 1h to form a 10% solution, adding the rest water, the extracellular polysaccharide and the activated carbon powder according to the mass ratio, stirring, and shearing and dispersing at 1500rpm for 5min by using a shearing machine to prepare the bio-organic fertilizer particle coating agent.
The prepared coating agent is used for coating the biological organic fertilizer to obtain granules with bright appearance.
3. Influence of coating agent on survival of functional microbial inoculum
In the test, the prepared coating agent is used as a test sample (sample 3), 5% polyvinyl alcohol solution (comparative sample 3-1) is used as a control sample, the selected test bacteria are commonly used bacillus mucilaginosus, and related equipment, vessels and culture media are subjected to normal sterilization treatment. The experimental method comprises the following steps: 3g of Bacillus mucilaginosus was added to 20g of the coating agent (sample 3) or the polyvinyl alcohol solution (comparative sample 3-1), shaken well, and left for 2 hours and 4 hours. In a laboratory fumigated by formaldehyde, an ultraviolet lamp is adopted to perform sterilization treatment on an ultra-clean workbench for more than half an hour. Coating with 2 hr or 4 hr coating agent or 5% polyvinyl alcohol solution, diluting, gradient coating, and sealing with flame, and collecting dilution gradient 107And 108And performing parallel coating for 4 times, putting the mixture into an incubator for 2 days at the constant temperature of 32-33 ℃, wherein the used culture medium is a nutrient agar culture medium, counting the number of colonies, and obtaining the results shown in tables 6 and 7. From tables 6 and 7, it is understood that Bacillus mucilaginosus was favorably survived in sample 3 and not favorably survived in comparative sample 3-1.
TABLE 6 dilution gradient 107Culture statistics of colony count
TABLE 7 dilution gradient 108Culture statistics of colony count
In conclusion, the bio-organic fertilizer particle coating agent can improve the appearance commodity of fertilizer products, enables the coated fertilizer to be more convenient and sanitary to use, is environment-friendly, has small soil pollution, can coexist with microorganisms, cannot cause short survival time of the microorganisms, has high long-time survival rate of effective viable bacteria of the coated bio-organic fertilizer, and is beneficial to fertilizer synergism and plant immunity improvement.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. The bio-organic fertilizer particle coating agent is characterized by comprising 20-25% of exopolysaccharide, 4.5-5.5% of polyvinyl alcohol, 0.5-1% of activated carbon powder and water.
2. The bio-organic fertilizer particle coating agent as claimed in claim 1, wherein the exopolysaccharide is prepared by mixing 0.9-1 part by weight of bacillus polymyxa exopolysaccharide, 5-5.5 parts by weight of bacillus mucilaginosus exopolysaccharide and 2.5-3 parts by weight of bacillus amyloliquefaciens exopolysaccharide.
3. The bio-organic fertilizer particle coating agent as claimed in claim 2, wherein a fermentation medium for fermenting the bacillus polymyxa exopolysaccharide comprises 60-70 g/L of sucrose, 30-40 g/L of peptone, 35-45 g/L of yeast powder, 0.5-10 g/L of monopotassium phosphate, 0.1-2 g/L of manganese sulfate, 0.2-2 g/L of ammonium nitrate, 0.05-0.1 g/L of sodium chloride and 0.05-0.1 g/L of ferric chloride.
4. The bio-organic fertilizer particle coating agent as claimed in claim 2, wherein a fermentation medium for fermenting the bacillus mucilaginosus exopolysaccharide comprises 30-50 g/L of sucrose, 0.4-8 g/L of monopotassium phosphate, 0.2-20 g/L of magnesium sulfate heptahydrate, 0.2-2 g/L of ammonium nitrate, 0.05-0.1 g/L of ferric chloride and 0.03-0.05 g/L of calcium carbonate.
5. The bio-organic fertilizer particle coating agent as claimed in claim 2, wherein a fermentation medium for fermenting the bacillus amyloliquefaciens exopolysaccharide comprises 20-40 g/L of maltose, 30-40 g/L of yeast powder, 0.3-6 g/L of diammonium citrate, 0.2-20 g/L of magnesium sulfate heptahydrate, 0.5-1 g/L of ammonium nitrate, 0.05-0.1 g/L of sodium chloride, 1-3 g/L of ammonium nitrate and 0.05-0.1 g/L of ferric chloride.
6. The bio-organic fertilizer particle coating agent as claimed in claim 1, wherein the polyvinyl alcohol has an average polymerization degree of 1000-1800 and an alcoholysis degree of 78-88%.
7. The bio-organic fertilizer particle coating agent as claimed in claim 1, wherein the particle size of the activated carbon powder is 200-300 mesh.
8. The bio-organic fertilizer particle coating agent as claimed in claim 1, wherein the diameter of the aperture of the activated carbon powder is 100-200 nm.
9. A preparation method of the bio-organic fertilizer particle coating agent as claimed in any one of claims 1 to 8, characterized in that, after polyvinyl alcohol is dissolved in water to form a solution with a mass percentage of 8-10%, the rest water, extracellular polysaccharide and activated carbon powder are added according to the mass ratio of each component respectively, and stirring, shearing and dispersing are carried out to prepare the bio-organic fertilizer particle coating agent.
10. The preparation method of the bio-organic fertilizer particle coating agent according to claim 9, wherein the temperature of water for dissolving the polyvinyl alcohol is 50-95 ℃, and the stirring and dissolving time is 1-4 hours.
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| CN113767825A (en) * | 2021-09-30 | 2021-12-10 | 广西壮族自治区农业科学院 | Method for improving plant growing rate of mechanically-harvested and rolled perennial sugarcane |
| CN115093287A (en) * | 2022-07-19 | 2022-09-23 | 临沂市冠宇工业科技有限公司 | Coating agent for microbial fertilizer and preparation method thereof |
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2021
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113767825A (en) * | 2021-09-30 | 2021-12-10 | 广西壮族自治区农业科学院 | Method for improving plant growing rate of mechanically-harvested and rolled perennial sugarcane |
| CN115093287A (en) * | 2022-07-19 | 2022-09-23 | 临沂市冠宇工业科技有限公司 | Coating agent for microbial fertilizer and preparation method thereof |
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