CN110981610A - Microbial agent with disease prevention function and preparation method thereof - Google Patents
Microbial agent with disease prevention function and preparation method thereof Download PDFInfo
- Publication number
- CN110981610A CN110981610A CN201911326579.XA CN201911326579A CN110981610A CN 110981610 A CN110981610 A CN 110981610A CN 201911326579 A CN201911326579 A CN 201911326579A CN 110981610 A CN110981610 A CN 110981610A
- Authority
- CN
- China
- Prior art keywords
- parts
- fermentation
- spore liquid
- microbial agent
- rape cake
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B3/00—Fertilisers based essentially on di-calcium phosphate
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a microbial agent with a disease prevention function, which comprises the following raw materials in parts by weight: 20-50 parts of protein powder, 20-50 parts of calcium magnesium phosphate fertilizer, 50-100 parts of calcium hydrophosphate, 3-5 parts of mannitol, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer 700-containing organic fertilizer and 5-10 parts of composite functional bacteria spore liquid. According to the principles of soil microecology and plant nutrition physiology, the invention selects high-efficiency microbial strains for preventing and treating tobacco bacterial wilt and black shank, and adopts modern microbial engineering technology to refine the microbial agent. The product can improve the quantity of beneficial microorganisms, effectively inhibit pathogenic bacteria and harmful bacteria, and solve the problems of aggravation of soil-borne diseases and reduction of yield and quality of flue-cured tobacco caused by continuous cropping of flue-cured tobacco by adjusting the composition and structure of microorganisms in soil, rhizosphere and roots, thereby achieving the purposes of developed tobacco root system, vigorous growth, strong stress resistance and improved quality.
Description
Technical Field
The invention relates to the field of microbial agents, in particular to a microbial agent with a disease prevention function and a preparation method thereof.
Background
The agricultural microbial agent is a live bacterial preparation prepared by processing target microorganisms or effective bacteria after industrial propagation, can be used for seed dressing and root irrigation, and has the effects of improving soil, restoring land fertility, maintaining rhizosphere microbial balance, degrading toxic substances and the like, so that the agricultural microbial agent is widely applied to planting of crops and improving soil. The agricultural microbial agent is divided into liquid, powder and particles according to dosage forms, and the liquid and the powder have the problems of difficult storage and incapability of effectively acting on the rhizosphere of crops in the production and application processes, thereby increasing the application cost of the agricultural microbial agent invisibly.
Disclosure of Invention
The invention aims to provide a microbial agent with a disease prevention function and a preparation method thereof aiming at the defects of the prior art, and the microbial agent is prepared by selecting high-efficiency microbial strains for preventing and treating tobacco bacterial wilt and black shank and refining by adopting the modern microbial engineering technology according to the principles of soil microecology and plant nutrition physiology. The product can improve the quantity of beneficial microorganisms, effectively inhibit pathogenic bacteria and harmful bacteria, and solve the problems of aggravation of soil-borne diseases and reduction of yield and quality of flue-cured tobacco caused by continuous cropping of flue-cured tobacco by adjusting the composition and structure of microorganisms in soil, rhizosphere and roots, thereby achieving the purposes of developed tobacco root system, vigorous growth, strong stress resistance and improved quality.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 20-50 parts of protein powder, 20-50 parts of calcium magnesium phosphate fertilizer, 50-100 parts of calcium hydrophosphate, 3-5 parts of mannitol, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer 700-containing organic fertilizer and 5-10 parts of composite functional bacteria spore liquid;
the functional bacteria in the composite functional bacteria spore liquid comprise bacillus amyloliquefaciens, and the preservation registration number is as follows: CGMCC NO.18262, Bacillus belgii, preservation registration number: CGMCC NO.18272, the volume ratio of the bacillus amyloliquefaciens to the bacillus beleisi spore liquid is 1-5: 1-5.
The preparation method comprises the following steps:
preparing spore liquid, namely inoculating bacillus amyloliquefaciens and bacillus belgii strains into a liquid fermentation culture medium respectively under an aseptic condition, culturing for 24-36h at 25-37 ℃, then inoculating the bacillus amyloliquefaciens and the bacillus belgii strains into the liquid fermentation culture medium again according to the volume ratio of 10-20%, performing amplification culture for 24-36h at 25-37 ℃, and centrifuging fermentation liquor of functional strains to obtain a lower layer spore liquid for later use;
step two, preparing a composite functional bacteria spore liquid, namely preparing the bacillus amyloliquefaciens and bacillus belgii spore liquid prepared in the step one according to the ratio of 1-5: 1-5, and preparing a composite functional bacterial spore solution for later use;
step three, preparing materials, namely respectively taking 20-50 parts of protein powder, mannitol, 3-5 parts of compound functional bacteria spore liquid and 5-10 parts of compound functional bacteria spore liquid, and uniformly mixing for later use;
step four, preparing materials, namely putting 50-100 parts of calcium hydrophosphate, 20-50 parts of calcium magnesium phosphate fertilizer, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate and 800 parts of rape cake organic fertilizer 700-plus materials into a mixing machine in proportion, and mixing uniformly for later use;
step five, granulating, namely adding water into molasses to prepare a solution with the concentration of 50%, taking the molasses solution and the prepared materials in the step three and the step four according to the weight ratio of 15:100, and granulating by using a disc granulator;
and step six, performing vacuum filtration and drying on the granules obtained in the step five, and screening to obtain a finished product.
Furthermore, the spore amount in the spore liquid prepared in the step one is more than 95 percent, and the effective viable count is more than or equal to 500 hundred million cfu/ml.
Further, the liquid fermentation medium in the first step comprises the following components: 10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride.
Further, the preparation method of the rape cake organic fertilizer comprises the following steps: crushing rape cake into 1-5mm pieces by a crusher, mixing the rape cake pieces, a fermentation decomposition agent and water according to the weight ratio of 60%, 0.5% and 39.5%, uniformly stirring, and performing groove type fermentation, wherein the temperature is not higher than 50 ℃ in the fermentation process, the fermentation period is 15 days, after fermentation and decomposition, the ripening process is performed for 10 days, the ripened rape cake is crushed by the crusher, and the organic fertilizer for the completely decomposed rape cake is obtained after sieving by a 40-mesh sieve.
The invention has the advantages that:
1. after the functional strains are applied to soil, the functional strains can be fast colonized on the soil and around rhizosphere to form dominant bacteria, and the action of pathogenic bacteria is inhibited.
2. The functional microorganism can effectively utilize carbohydrates such as sugar in molasses, can effectively utilize byproducts generated after sugar production of sugarcane, and meanwhile, the viscosity of the functional microorganism is favorable for improving the granulation and balling rate and saving the production cost.
3. The protein powder, mannitol and other substances have positive effects on maintaining microbial activity and prolonging shelf life.
4. The microbial agent refined by modern microbial engineering technology improves the number of beneficial microorganisms by adjusting the composition and structure of soil, rhizosphere and intraradicular microorganisms, effectively inhibits pathogenic bacteria and harmful bacteria, and solves the problems of aggravation of soil-borne diseases and reduction of yield and quality of flue-cured tobacco caused by continuous cropping of flue-cured tobacco, thereby achieving the purposes of developed root system, vigorous growth, strong stress resistance and improved quality of tobacco.
Detailed Description
The present invention is further illustrated by the following examples, but is not limited to the details of the description.
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 20-50 parts of protein powder, 20-50 parts of calcium magnesium phosphate fertilizer, 50-100 parts of calcium hydrophosphate, 3-5 parts of mannitol, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer 700-containing organic fertilizer and 5-10 parts of composite functional bacteria spore liquid.
The organic fertilizer of the rape cake is decomposed and then crushed and sieved by a 40-mesh sieve.
The functional bacteria in the composite functional bacteria spore liquid comprise bacillus amyloliquefaciens (Bacillus amyloliquefaciens), and the preservation registration number is as follows: CGMCC NO.18262, preservation unit: china general microbiological culture Collection center, preservation Address: west road No.1 hospital No. 3, north jing, chaoyang district, preservation date: 7 and 22 months in 2019.
Bacillus belgii (Bacillus velezensis), accession number: CGMCC NO.18272, preservation unit: china general microbiological culture Collection center, preservation Address: west road No.1 hospital No. 3, north jing, chaoyang district, preservation date: 7 and 22 months in 2019.
The preparation method comprises the following steps:
(1) preparing spore liquid, namely inoculating bacillus amyloliquefaciens and bacillus belgii strains into a liquid fermentation culture medium respectively under an aseptic condition, culturing for 24-36h under the condition of 25-37 ℃, then inoculating the bacillus amyloliquefaciens and the bacillus belgii strains into a new liquid fermentation culture medium again according to the volume ratio of 10-20% (strains and fermentation liquid), performing amplification culture for 24-36h under the condition of 25-37 ℃, and then centrifuging the fermentation liquid of the functional strains to obtain the lower spore liquid for later use;
(2) preparing a composite functional bacterial spore solution, namely preparing the spore solution prepared in the step (1), wherein the spore amount is more than 95%, the effective viable count is more than or equal to 500 hundred million cfu/ml, and the spore solution of the bacillus amyloliquefaciens and the bacillus belgii is prepared according to the proportion of 1-5: 1-5, and preparing a composite functional bacterial spore solution;
(3) preparing materials, respectively taking 20-50 parts of protein powder, mannitol, and composite functional bacterial spore liquid, 3-5 parts of composite functional bacterial spore liquid and 5-10 parts of composite functional bacterial spore liquid, and uniformly mixing the components for later use.
(4) 50-100 parts of calcium hydrogen phosphate, 20-50 parts of calcium magnesium phosphate fertilizer, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate and 800 parts of rape cake organic fertilizer 700-.
(5) And (4) granulating, namely adding water into molasses to prepare a solution with the concentration of 50 percent (mass) for later use, and granulating the solution with the concentration of 50 percent and the prepared materials in the steps (3) and (4) by a disk granulator according to the weight ratio of 15 percent. The water content is controlled to be maintained between 20 percent and 25 percent all the time in the granulation process, and the rotating speed of a disc is controlled to be 10 to 12 r/min.
(6) And (5) granulating, then carrying out suction filtration to achieve vacuum drying, and screening to obtain a finished product. The filtration temperature is set to 37 ℃ to ensure the survival rate of the strains, the air release valve is closed, the vacuum valve is opened for 20min, and the strains are sieved by a 5-8-mesh sieve.
The liquid fermentation culture medium comprises the following components: 10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride.
The preparation method of the rape dry cake organic fertilizer comprises the following steps: crushing rape cake into 1-5mm pieces, mixing the rape cake pieces, fermentation decomposition agent and water at a weight ratio of 60%, 0.5% and 39.5%, stirring, performing tank fermentation, and starting an aeration device (0.1 m per cubic meter of material)3Min) and turning and throwing once every 2 days after temperature rise, maintaining the oxygen content between 15 percent and 20 percent in the fermentation process, the temperature is not higher than 50 ℃, the fermentation period is 15 days, after complete fermentation and decomposition, carrying out the curing process for about 10 days, crushing the cured rape cake by a crusher, and sieving by a 40-mesh sieve to obtain the complete decomposed rape cake organic fertilizer.
Detailed description of the preferred embodiment 1
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 20 parts of protein powder, 50 parts of calcium magnesium phosphate fertilizer, 50 parts of calcium hydrogen phosphate, 3 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 1 part of magnesium sulfate heptahydrate, 700 parts of rape cake organic fertilizer and 5 parts of composite functional bacteria spore liquid.
Detailed description of the preferred embodiment 1
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 20 parts of protein powder, 20 parts of calcium magnesium phosphate fertilizer, 50 parts of calcium hydrophosphate, 3 parts of mannitol, 1 part of ferrous sulfate heptahydrate, 1 part of magnesium sulfate heptahydrate, 700 parts of rape cake organic fertilizer and 5 parts of composite functional bacteria spore liquid.
Specific example 2
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 50 parts of protein powder, 50 parts of calcium magnesium phosphate fertilizer, 100 parts of calcium hydrophosphate, 5 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer and 10 parts of composite functional bacteria spore liquid.
Specific example 3
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 35 parts of protein powder, 35 parts of calcium magnesium phosphate fertilizer, 75 parts of calcium hydrophosphate, 4 parts of mannitol, 2 parts of ferrous sulfate heptahydrate, 2 parts of magnesium sulfate heptahydrate, 750 parts of rape cake organic fertilizer and 8 parts of composite functional bacteria spore liquid.
Specific example 4
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 20 parts of protein powder, 25 parts of calcium magnesium phosphate fertilizer, 100 parts of calcium hydrophosphate, 5 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 1 part of magnesium sulfate heptahydrate, 750 parts of rape cake organic fertilizer which is completely decomposed, crushed and sieved by a 40-mesh sieve and 5 parts of composite functional bacteria spore liquid.
Specific example 5
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 50 parts of protein powder, 40 parts of calcium magnesium phosphate fertilizer, 60 parts of calcium hydrophosphate, 3 parts of mannitol, 1 part of ferrous sulfate heptahydrate, 3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer which is completely decomposed, crushed and sieved by a 40-mesh sieve and 10 parts of composite functional bacteria spore liquid.
Specific example 6
A microbial agent with a disease prevention function comprises the following raw materials in parts by weight: 40 parts of protein powder, 50 parts of calcium magnesium phosphate fertilizer, 80 parts of calcium hydrophosphate, 4 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer which is completely decomposed, crushed and sieved by a 40-mesh sieve and 5 parts of composite functional bacteria spore liquid.
The following are specific application effect tests
1.2 different proportions of disease-preventing microbial agents have greenhouse disease-preventing effect on tobacco bacterial wilt
1.2.1 test site
Greenhouse of the research center for microbial fermentation engineering of Yunnan province, Ltd.
1.2.2 test crops
And (4) cloud smoke 87.
1.2.3 test design
After tobacco seeds are placed in a plastic plug tray for seedling cultivation, tobacco seedlings with consistent growth in the three-leaf period are transplanted into a greenhouse, the tobacco is treated when the tobacco enters a vigorous growing period, 23g (25 kg/mu) of microbial inoculum is applied to each plant of the biological control agent after membrane uncovering, then soil is covered, and a control group is irrigated with equal volume of clear water. The test treatments are respectively the biocontrol microbial inoculum of examples 1-6, the corresponding treatments of No. 1-6 of examples 1-6 are 1-6, 25 tobacco seedlings are treated in each treatment group, and each treatment is repeated for 3 times. After being treated by the test microbial inoculum for 7 days, 30ml of bacterial wilt pathogenic bacteria are irrigated to each strain, so that the bacterial density is maintained at 3.0-5.0 x 107cuf/ml. After the treatment of pathogenic bacteria, the growth condition and the morbidity of the pathogenic bacteria are observed every day, and the final result is counted after 30 days.
1.2.4 different proportions of biocontrol microbial inoculum for controlling tobacco bacterial wilt in greenhouse
As can be seen from Table 1, different treatment inoculants are selected as biocontrol potential strains to carry out greenhouse control effect tests on tobacco bacterial wilt, after 8 days of inoculation of pathogenic bacteria, the tobacco plants in a control group begin to suffer from diseases, leaf withering at the bottom of the plants occurs, the disease-sensitive time of each treatment in the test group is relatively delayed, after 30 days of inoculation of the pathogenic bacteria, the disease-sensitive degree of each treatment is statistically analyzed, each biocontrol bacterium treatment has a certain control effect on the tobacco bacterial wilt, wherein the control effect of the inoculant 3 is optimally 76.22%, the control effect of the inoculant 5 is the worst, and the control effect is 73.90%.
Greenhouse control effect of test strains of Table 16 on tobacco bacterial wilt
Note: different letters indicate a difference with a 0.05 significance
1.3 greenhouse prevention of test strains on tobacco Black shank
1.3.1 test site
Open-air test field outside the greenhouse of Limited company of research center of microbial fermentation engineering in Yunnan province.
1.3.2 test crops
And (4) cloud smoke 87.
1.3.3 test design
Selecting plots with continuously-attacked black shank of tobacco in an open-air test, setting 8 treatments, using 6 biocontrol microbial inoculum with different proportions as treatments, using 58% metalaxyl manganese zinc (mixed with 100kg of water in each bag) as a positive control, using the control without any medicament treatment as a blank control, wherein the test treatments are respectively the biocontrol microbial inoculum of examples 1-6, the corresponding treatments of examples 1-6 are repeated for 3 times, 30 cigarettes are planted in each cell, and all the treatments are randomly arranged in blocks. The treatment concentration and dosage of the medicament are described by reference to the medicament, and the tobacco seedlings are applied for 2 times when entering the vigorous growth period; applying and covering soil after uncovering the film, wherein the field cultivation management method is consistent with that of the local area, no other bactericide is applied, and the prevention effect statistics and analysis are carried out 30 days after the tobacco enters the vigorous growth period.
1.3.4 field control effect of biocontrol microbial inoculum with different proportions on tobacco black shank
As can be seen from Table 2, when the tobacco black shank plot test is carried out by using 6 different proportions of the biocontrol microbial inoculum, each biocontrol microbial inoculum has a certain control effect, wherein the control effect of the microbial inoculum 3 is optimal and reaches 69.74%, and then the microbial inoculum 2, the microbial inoculum 1, the microbial inoculum 4, the microbial inoculum 6 and the microbial inoculum 5 have better control effects than chemical agents, but are very ideal as the control effects of the biocontrol agents.
TABLE 2 field control of different biocontrol microbial agents on tobacco black shank
| Treatment of | The incidence of disease% | Index of disease condition | The control effect is% |
| 1 | 11.33e | 3.40e | 65.43±0.15b |
| 2 | 9.66f | 4.86b | 65.70±1.45g |
| 3 | 16.33c | 4.86b | 69.74±0.39e |
| 4 | 14.0d | 4.46c | 62.73±0.40c |
| 5 | 16.33c | 4.73bc | 61.03±0.21d |
| 6 | 11.0e | 3.86d | 62.26±0.21c |
| 58% metalaxyl manganese zinc | 2.33g | 1.00f | 88.74±0.84a |
| CK | 23.66a | 6.73a | - |
Note: different letters indicate a difference with a 0.05 significance
The results are combined to show that: the biocontrol microbial inoculum prepared by the 2 functional bacteria has good control effect on tobacco bacterial wilt and black shank, has ideal control effect compared with chemical agents with the same function, and can be widely applied to the field of biological control of tobacco diseases.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (5)
1. A microbial agent with disease prevention function is characterized in that: the composite material comprises the following raw materials in parts by weight: 20-50 parts of protein powder, 20-50 parts of calcium magnesium phosphate fertilizer, 50-100 parts of calcium hydrophosphate, 3-5 parts of mannitol, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate, 800 parts of rape cake organic fertilizer 700-containing organic fertilizer and 5-10 parts of composite functional bacteria spore liquid;
the functional bacteria in the composite functional bacteria spore liquid comprise bacillus amyloliquefaciens, and the preservation registration number is as follows: CGMCC NO.18262, Bacillus belgii, preservation registration number: CGMCC NO.18272, the volume ratio of the bacillus amyloliquefaciens to the bacillus beleisi spore liquid is 1-5: 1-5.
2. The microbial agent with disease prevention function according to claim 1, wherein: the preparation method comprises the following steps:
preparing spore liquid, namely inoculating bacillus amyloliquefaciens and bacillus belgii strains into a liquid fermentation culture medium respectively under an aseptic condition, culturing for 24-36h at 25-37 ℃, then inoculating the bacillus amyloliquefaciens and the bacillus belgii strains into the liquid fermentation culture medium again according to the volume ratio of 10-20%, performing amplification culture for 24-36h at 25-37 ℃, and centrifuging fermentation liquor of functional strains to obtain a lower layer spore liquid for later use;
step two, preparing a composite functional bacteria spore liquid, namely preparing the bacillus amyloliquefaciens and bacillus belgii spore liquid prepared in the step one according to the ratio of 1-5: 1-5, and preparing a composite functional bacterial spore solution for later use;
step three, preparing materials, namely respectively taking 20-50 parts of protein powder, mannitol, 3-5 parts of compound functional bacteria spore liquid and 5-10 parts of compound functional bacteria spore liquid, and uniformly mixing for later use;
step four, preparing materials, namely putting 50-100 parts of calcium hydrophosphate, 20-50 parts of calcium magnesium phosphate fertilizer, 1-3 parts of ferrous sulfate heptahydrate, 1-3 parts of magnesium sulfate heptahydrate and 800 parts of rape cake organic fertilizer 700-plus materials into a mixing machine in proportion, and mixing uniformly for later use;
step five, granulating, namely adding water into molasses to prepare a solution with the concentration of 50%, taking the molasses solution and the prepared materials in the step three and the step four according to the weight ratio of 15:100, and granulating by using a disc granulator;
and step six, performing vacuum filtration and drying on the granules obtained in the step five, and screening to obtain a finished product.
3. The microbial agent with disease prevention function according to claim 2, wherein: the spore amount in the spore liquid prepared in the first step is more than 95 percent, and the effective viable count is more than or equal to 500 hundred million cfu/ml.
4. The microbial agent with disease prevention function according to claim 2, wherein: the liquid fermentation culture medium in the first step comprises the following components: 10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride.
5. The microbial agent with disease prevention function according to claim 1, wherein: the preparation method of the rape cake organic fertilizer comprises the following steps: crushing rape cake into 1-5mm pieces by a crusher, mixing the rape cake pieces, a fermentation decomposition agent and water according to the weight ratio of 60%, 0.5% and 39.5%, uniformly stirring, and performing groove type fermentation, wherein the temperature is not higher than 50 ℃ in the fermentation process, the fermentation period is 15 days, after fermentation and decomposition, the ripening process is performed for 10 days, the ripened rape cake is crushed by the crusher, and the organic fertilizer for the completely decomposed rape cake is obtained after sieving by a 40-mesh sieve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911326579.XA CN110981610A (en) | 2019-12-20 | 2019-12-20 | Microbial agent with disease prevention function and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911326579.XA CN110981610A (en) | 2019-12-20 | 2019-12-20 | Microbial agent with disease prevention function and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110981610A true CN110981610A (en) | 2020-04-10 |
Family
ID=70074055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911326579.XA Pending CN110981610A (en) | 2019-12-20 | 2019-12-20 | Microbial agent with disease prevention function and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110981610A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111411063A (en) * | 2020-05-15 | 2020-07-14 | 云南省微生物发酵工程研究中心有限公司 | Bacillus composite microbial inoculum with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
| CN111807898A (en) * | 2020-05-15 | 2020-10-23 | 云南省微生物发酵工程研究中心有限公司 | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
| CN112586509A (en) * | 2020-11-13 | 2021-04-02 | 遵义医科大学 | Compound microorganism effervescent tablet for preventing tobacco bacterial wilt and promoting growth, and preparation method and application thereof |
| CN113416104A (en) * | 2021-06-10 | 2021-09-21 | 中国农业科学院油料作物研究所 | Spraying type preparation for biologically preventing sclerotinia and promoting high yield of rape and preparation method thereof |
| CN114907164A (en) * | 2021-12-30 | 2022-08-16 | 云南云叶化肥股份有限公司 | Bio-organic fertilizer capable of preventing and treating tobacco soil-borne diseases, preparation method and application |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531588A (en) * | 2015-01-07 | 2015-04-22 | 南京农业大学 | Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain |
| CN107779420A (en) * | 2017-06-27 | 2018-03-09 | 湖北省烟草公司恩施州公司 | A kind of Nei Shengbeilaisi bacillus of two plants of antagonism tobacco bacterial wilts and its application |
| CN108004185A (en) * | 2018-01-09 | 2018-05-08 | 中国农业科学院植物保护研究所 | One plant of tool diseases prevention, growth-promoting, drought resisting function plant endogenesis Bei Laisi bacillus and its application |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| CN110268847A (en) * | 2019-06-05 | 2019-09-24 | 云南大学 | One kind exempting from the direct method of administration of decomposed raw grouts fertilizer |
-
2019
- 2019-12-20 CN CN201911326579.XA patent/CN110981610A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104531588A (en) * | 2015-01-07 | 2015-04-22 | 南京农业大学 | Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain |
| CN107779420A (en) * | 2017-06-27 | 2018-03-09 | 湖北省烟草公司恩施州公司 | A kind of Nei Shengbeilaisi bacillus of two plants of antagonism tobacco bacterial wilts and its application |
| CN108004185A (en) * | 2018-01-09 | 2018-05-08 | 中国农业科学院植物保护研究所 | One plant of tool diseases prevention, growth-promoting, drought resisting function plant endogenesis Bei Laisi bacillus and its application |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| CN110268847A (en) * | 2019-06-05 | 2019-09-24 | 云南大学 | One kind exempting from the direct method of administration of decomposed raw grouts fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| 施河丽等: "烟草黑胫病拮抗芽孢杆菌的筛选及生防效果评价", 《湖北农业科学》 * |
| 薛超群等: "《烟草病虫害绿色防控》", 31 August 2018, 河南科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111411063A (en) * | 2020-05-15 | 2020-07-14 | 云南省微生物发酵工程研究中心有限公司 | Bacillus composite microbial inoculum with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
| CN111807898A (en) * | 2020-05-15 | 2020-10-23 | 云南省微生物发酵工程研究中心有限公司 | Bio-organic fertilizer with function of preventing and treating tobacco soil-borne diseases and preparation method thereof |
| CN112586509A (en) * | 2020-11-13 | 2021-04-02 | 遵义医科大学 | Compound microorganism effervescent tablet for preventing tobacco bacterial wilt and promoting growth, and preparation method and application thereof |
| CN113416104A (en) * | 2021-06-10 | 2021-09-21 | 中国农业科学院油料作物研究所 | Spraying type preparation for biologically preventing sclerotinia and promoting high yield of rape and preparation method thereof |
| CN114907164A (en) * | 2021-12-30 | 2022-08-16 | 云南云叶化肥股份有限公司 | Bio-organic fertilizer capable of preventing and treating tobacco soil-borne diseases, preparation method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110981610A (en) | Microbial agent with disease prevention function and preparation method thereof | |
| KR101039176B1 (en) | Antagonistic bacteria for preventing and eliminating the bacterial wilt of continuous cropping tobacco and their microbial organic fertilizer | |
| CN102515951B (en) | Tobacco composite microbial fertilizer and its preparation method | |
| CN109650986B (en) | Carbon-based bio-organic fertilizer for improving degraded vegetable field soil and application thereof | |
| CN110616171B (en) | Saline-alkali-resistant Pacific bacillus and viable bacteria preparation and application thereof | |
| CN113621532B (en) | Composite microbial agent containing saline-alkali tolerant side bacillus licheniformis and preparation method thereof | |
| CN111793498A (en) | Microbial agent and preparation method and application thereof | |
| CN105875645A (en) | Preparing method of biological agent for preventing and controlling tobacco root black rot | |
| CN104911129A (en) | Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof | |
| CN105505836A (en) | Bacillus subtilis and methods for preparing and using bacillus subtilis microbial agent for controlling potato scab | |
| CN101886055B (en) | Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt | |
| CN111808783B (en) | Enterobacter cloacae and production method and application of viable bacteria preparation thereof | |
| CN115197023B (en) | Bio-organic fertilizer with landscaping waste as matrix and preparation method thereof | |
| CN111172066A (en) | Bacillus megaterium and application thereof | |
| CN113233937A (en) | Bio-organic fertilizer particle coating agent and preparation method thereof | |
| CN110791459B (en) | Bacillus subtilis for preventing and controlling continuous cropping lily soil-borne blight and application thereof | |
| CN111088169B (en) | Trichoderma, microbial agent and application thereof | |
| CN109136144B (en) | Special compound biological agent for ginseng and production and preparation method thereof | |
| CN115960766A (en) | Microorganism for preventing and treating bacterial wilt and application thereof | |
| CN110791445B (en) | Bacillus amyloliquefaciens and biological pesticide fertilizer | |
| CN112795518A (en) | Disease-resistant and quality-improving microbial agent special for tobacco and application thereof | |
| CN107227270A (en) | A kind of biocontrol bacteria in soil-borne diseases agent, a kind of soil-borne disease biological control matrix and preparation method thereof and application method | |
| CN108949635B (en) | Bacillus mucilaginosus, microbial fertilizer, preparation method and application thereof | |
| CN111849843A (en) | Application of Siamese bacillus B11 in prevention and/or treatment of bamboo shoot dry blight | |
| CN110683875A (en) | Biological bacterial fertilizer for preventing bacterial wilt of flue-cured tobacco, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200410 |
|
| RJ01 | Rejection of invention patent application after publication |