CN113229397A - Method and equipment for producing solid microbial fermented feed through roller fermentation - Google Patents

Method and equipment for producing solid microbial fermented feed through roller fermentation Download PDF

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Publication number
CN113229397A
CN113229397A CN202110206399.9A CN202110206399A CN113229397A CN 113229397 A CN113229397 A CN 113229397A CN 202110206399 A CN202110206399 A CN 202110206399A CN 113229397 A CN113229397 A CN 113229397A
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feed
fermentation
roller
parts
pipeline
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CN113229397B (en
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鲁耀雄
彭福元
蒋德光
崔新卫
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Hunan Institute Of Agro-Environment And Ecology
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Hunan Institute Of Agro-Environment And Ecology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
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    • A23K—FODDER
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    • A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • A—HUMAN NECESSITIES
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    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/35—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from potatoes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
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    • A23N17/007—Apparatus specially adapted for preparing animal feeding-stuffs for mixing feeding-stuff components
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16—Solid state fermenters, e.g. for koji production
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    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract

A method and equipment for producing solid microbial fermentation feed by roller fermentation relate to the technical field of feed production. The method comprises the following steps: 1. mixing the raw materials such as straw and the like according to a scientific ratio, and then inputting the mixture into a sterilization tank for sterilization; 2. mixing the sterilized raw materials with a microbial agent, inputting the mixture into a roller dynamic fermentation reactor, and fermenting the solid microbial fermented feed; 3. the central control system intelligently regulates and controls the relevant parameters of the dynamic fermentation reactor of the roller; 4. and (3) discharging, detecting, granulating and packaging the solid microbial fermented feed. The invention has the beneficial effects that: the solid microbial fermentation feed can be produced by utilizing crop straws such as rice straws, corns and the like, is beneficial to high-value utilization of the feed of crop straw resources, and promotes the sustainable development of circular agriculture; the horizontal roller is a dynamic fermentation process which simultaneously carries out feeding and discharging, and the continuous production of the microbial fermented feed is realized.

Description

Method and equipment for producing solid microbial fermented feed through roller fermentation
Technical Field
The invention relates to the technical field of feed production, in particular to a method and equipment for producing solid microbial fermented feed by roller fermentation.
Background
China is a big livestock and poultry breeding country and also a big country for producing and using antibiotics for livestock. The antibiotic for animals plays an important role in preventing and treating animal diseases, improving breeding benefits and ensuring the effective supply of livestock and poultry products. However, the abuse of antibiotics for livestock causes problems of animal intestinal flora imbalance, livestock organism immunity reduction, drug residues, bacterial drug resistance and the like to become serious, seriously threatens the quality safety and public health safety of livestock products, and brings hidden troubles to human and animal health. The European Union forbids antibiotics from 2006 to be added into the livestock and poultry feed comprehensively, and the types of antibiotics allowed to be added into the feed in China are gradually reduced. Therefore, the application of antibiotics in the livestock industry is absolutely necessary, and the natural feed additive which has the advantages of growth promotion, no drug resistance, no toxic or side effect and no residue is searched as an antibiotic substitute product, so that the natural feed additive becomes the inevitable trend of livestock and poultry breeding development and the responsibility of irremovable industry customs.
The microbial fermentation feed contains various active probiotics, is green and environment-friendly, contains secondary metabolites and functional small peptides, can effectively slow down the growth of harmful bacteria, can remarkably improve the growth performance of animals to guarantee the health of intestinal tracts, and is expected to reduce or replace the use of feed antibiotics to a certain extent (such as Lijinku, 2019). The microbial fermented feed is prepared by taking microbes as a feed fermentation microbial inoculum under the condition of artificial control, decomposing or converting macromolecular substances such as polysaccharide, protein, fat and the like in the fermented feed raw materials to generate various nutrient substances such as organic acid, vitamin, amino acid and the like, and also can generate light fragrance in the fermentation process, thereby increasing the palatability of animals, regulating the intestinal microecological balance of the animals, improving the immunity of organisms and improving the resistance of the animals (Yubao, 2019). Although microbial fermented feed has many advantages, the existing development process has some problems, mainly the activation and propagation of the existing microbial feed additive are mostly carried out in simple equipment by a manual mode, the Chinese invention patent No. CN 207100479U (2018) is a rapid activation and propagation device of the microbial feed additive, the device is connected with a motor through a transmission rod in a tank body and drives a stirring rod to realize the propagation of the microbial feed additive, and the defects are that the stirring rod is directly contacted with the feed additive in the tank body, the abrasion is large, and the motor is easily blocked and even burnt and damaged in the tank body. In the traditional Chinese patent application CN 109622126A (2019), a microbial fermenter for feed production is arranged at the bottom in a frame and is in a static state, and the problems of nonuniform microbial fermentation and the like can be caused because of no stirring and temperature control processes. The invention adopts a horizontal roller to carry out dynamic continuous fermentation, controls the fermentation process by adjusting the temperature, ventilating, pH and other modes, and ensures the rapid propagation and uniformity of microorganisms, thereby improving the microbial activity in the feed, being beneficial to the efficient degradation and conversion of macromolecular substances, palatability and the like, and providing a method and equipment for producing solid microbial fermentation feed by roller fermentation.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art and provide a method and equipment for producing solid microbial fermentation feed by roller fermentation, which can produce microbial fermentation feed by utilizing crop straws such as straws, corns and the like and agricultural product byproducts, save the investment cost of a microbial agent by dynamic fermentation, improve the effective viable count and activity of microorganisms, increase the content of crude protein, reduce the content of crude fiber, effectively degrade macromolecular substances such as polysaccharide, protein, fat and the like in the feed, produce small molecular substances such as organic acid, polypeptide, amino acid and the like, produce antibacterial substances such as polypeptide, subtilisin, lipid substances and the like, and improve the quality of the microbial fermentation feed.
In order to achieve the purpose, the invention adopts the following technical scheme: a method for producing solid microbial fermented feed by roller fermentation is characterized by comprising the following steps:
respectively mixing 15-90 parts of straw powder and/or crushed chaff and/or rice bran and/or corn straw and/or corn cob and/or wheat straw and other crop straws, 2-25 parts of edible fungus residues and/or sweet potato residues and/or cassava residues and/or bean residues and/or kudzu vine residues and/or vinasse and/or potato residues and/or fruit residues and other agricultural and sideline processing residues, 2-25 parts of bran and/or corn bran and/or soybean hull and/or corn germ meal, 0.5-25 parts of soybean meal and/or peanut meal and/or cotton seed meal and/or rapeseed meal and other plant source protein raw materials, 0.5-15 parts of corn meal and/or corn molasses and/or soybean molasses and/or cane molasses and/or fermented molasses and/or glucose, 0.1-5 parts of medium trace elements are respectively loaded into different raw material hoppers 1, the raw materials are stirred by a first conveying mechanism 2 and are added with water to ensure that the water content of the materials is 45-60%, the raw materials are input into a sterilization container 4 to be sterilized by normal pressure steam (by adopting circulating steam at 100 ℃ for 8-12 hours) or by normal pressure intermittent steam (by adopting circulating steam at 100 ℃ for 1 hour, then being stored at 20-25 ℃ for 24 hours or being overnight at normal temperature, then the sterilization is carried out by the previous conditions for three times), and the medium trace elements are calcium carbonate, calcium superphosphate, sodium chloride, magnesium sulfate, zinc sulfate, borax, copper sulfate, manganese sulfate, potassium iodide and ferrous sulfate according to the mass ratio of 0.2-15: 0.2-10: 0.2-10: 0.1-10: 0.1-5: 0.1-5: 0.1-4: 0-2: 0-2: 0 to 2, and mixing.
And secondly, sterilizing and cooling the raw materials to below 35 ℃, conveying the raw materials into a roller dynamic fermentation feed reactor 8 through a first material output pipeline 44, simultaneously adding 0.1-2.0% of microbial agent, feeding fermentation material which is 70-80% of the volume of the roller for the first time, designing the frequency of the roller according to the process requirement of feed fermentation, continuously rolling the roller dynamic fermentation feed reactor 8, discharging the material according to the fermentation condition after 3-10 days, feeding and discharging the material every day according to the fermentation period, mixing and propelling the material through the rolling of the roller, and keeping the dynamic balance of the material of the roller dynamic fermentation feed reactor. The microbial agent is bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus coagulans, bacillus natto, bacillus cereus, bacillus pumilus, candida utilis, saccharomyces cerevisiae, rhizopus, aspergillus niger, aspergillus oryzae and white rot fungi, and the ratio of effective viable bacteria is 0-10: 0-10: 0-10: 0-8: 0-8: 0-8: 0-8: 0-8: 0-8: 0-5: 0-5: 0-5: 0-5, and mixing uniformly for later use, wherein the strains are strains for commercial feed.
And thirdly, the central control system 91 is utilized to adjust the rotation speed, the ventilation temperature, the strength, the heat feedback and other index parameters of the roller to keep the roller in a dynamic balance fermentation state, the temperature of the fermented feed 0.5 cm away from the feed inlet is about 30-52 ℃ (the specific temperature is determined according to the characteristics of matched roller equipment, strains and fermented products), and the temperature of the fermented feed 0.5 cm away from the discharge outlet is about 35-60 ℃ (the specific temperature is determined according to the characteristics of matched roller equipment, strains and fermented products).
Step four, when discharging every day, opening a baffle of a roller dynamic fermented feed reactor (8) and a discharging conveying device through a central control system 91 to output materials to obtain fermented microbial feed, and after relevant indexes are qualified through inspection, adding 0.5-10% (calculated according to dry basis, the same applies below) of fermented microbial feed into other mixed feed to feed livestock (the placing time is not more than 15 days) or taking 5-30% of fermented microbial feed to mix with other dry feed extrusion granulator 9 to granulate and feed poultry livestock, wherein the dry feed is: 10-75 parts of rice bran, 8-55 parts of corn flour, 5-30 parts of soybean meal, 2-20 parts of bran, 0-15 parts of barley, 0.5-10 parts of fish meal and/or earthworm powder and/or insect powder, 0-5 parts of bone meal, 0-2 parts of sodium bicarbonate (baking soda), 0-1 part of lysine, 0-0.5 part of methionine, 0-0.5 part of cystine, 0-0.5 part of tryptophan, 0-0.1 part of vitamin A, 0-0.1 part of vitamin D and 0-0.1 part of vitamin E.
The utility model provides an equipment of solid microorganism fermentation feed is produced in cylinder fermentation which characterized in that: the device comprises a raw material hopper 1, a conveying mechanism 2, a stirrer 3, a sterilization tank 4, a steam generator 5, a radiator 6, a waste gas processor 7, a roller dynamic fermentation feed reactor 8, an extrusion granulator 9, a screen 10, a packaging machine 11 and a powder recoverer 12, wherein the bottom of the raw material hopper 1 is provided with the first conveying mechanism 2, the first conveying mechanism 2 is provided with a conveying belt 21, the output end of the first conveying mechanism 2 is provided with the stirrer 3, the stirrer 3 is connected with the sterilization tank 4 through a second conveying mechanism 22, the stirrer 3 is connected with the extrusion granulator 9 through a third conveying mechanism 23, the bottom of the sterilization tank 4 is provided with the steam generator 5, the sterilization tank 4 is provided with a waste gas output pipeline 41, the steam generator 5 is provided with a steam pipeline 51 connected with the waste gas output pipeline 41, the output end of the waste gas output pipeline 41 is connected with a radiator 6, the output end of the radiator 6 is provided with two ends, one end is connected with a waste gas processor 7, the other end is connected with a roller dynamic fermentation feed reactor 8 through a heat feedback pipeline 42, the waste gas output pipeline 41 is also provided with a waste gas recovery pipeline 43, the output end of the waste gas recovery pipeline 43 is connected with the roller dynamic fermentation feed reactor 8, a first material output pipeline 44 is arranged between the sterilization tank 4 and the roller dynamic fermentation feed reactor 8, the upper part of the sterilization tank 4 is provided with a water pipe 46, the roller dynamic fermentation feed reactor 8 is connected with an extrusion granulator 9, the right side of the extrusion granulator 9 is connected with a powder recoverer 12, and the joint between the extrusion granulator 9 and the powder recoverer 12 is provided with a packing machine 11, a screen mesh 10 is arranged above the packaging machine 11.
The steam pipeline 51, the waste gas output pipeline 41 and the waste gas recovery pipeline 43 are provided with check valves 13.
The dynamic fermentation feed reactor 8 of the roller is provided with a temperature sensor 84 with a plurality of point positions, one end of the dynamic fermentation feed reactor 8 of the roller is provided with a front end fixed circular sealing cap 85, the other end is provided with a rear end fixed circular sealing cap 86, the front end fixed circular sealing cap 85 is provided with a feed hopper 851, the side of the feed hopper 851 is provided with an air inlet input pipeline 852 and a first fan 853, the air inlet input pipeline 852 is connected with the first fan 853, the periphery of the dynamic fermentation feed reactor 8 of the roller is provided with an outer layer heating and heat insulating system 854 of the roller, one side of the rear end fixed circular sealing cap 86 is provided with a guide plate 861, the other side is provided with an exhaust gas output pipeline 862 and a second fan 863, the exhaust gas output pipeline 862 is connected with the second fan 863, the bottom of the exhaust gas output pipeline 862 is connected with a discharge hopper 865 through a connecting rod 864, a discharge baffle 866 is arranged on the discharge hopper 865, a pair of driving wheels 87 is arranged at the bottom of the roller dynamic fermentation feed reactor 8, a driving shaft 88 is connected between the driving wheels 87, and a driving motor 89 is arranged at the middle bottom of the driving shaft 88.
Temperature-sensing ware 84 can know the fermentation temperature in real time, stock guide 861 be used for guiding the ejection of compact, outer heating heat preservation system 854 of cylinder can carry out temperature intelligent control to the fermentation.
The device for producing the solid microbial fermented feed through the roller fermentation is electrically connected with a central control system 91, the central control system 91 is connected with all components, the normal operation of all the components is monitored through a temperature sensor 84 and an air management system, and relevant data parameters are adjusted to control the fermentation efficiency.
After the technical scheme is adopted, the invention has the beneficial effects that: the solid microbial fermentation feed can be produced by utilizing crop straws such as rice straws, corns and the like, is beneficial to high-value utilization of the feed of crop straw resources, and promotes the sustainable development of circular agriculture; the horizontal roller is a dynamic fermentation process for simultaneously feeding and discharging, so that the continuous production of microbial fermented feed is realized, and the efficiency is high; the horizontal roller can effectively and quickly propagate and occupy the advantages only by throwing the microbial strains once, and the growth and propagation of the microbial strains are stabilized by scientifically preparing raw materials and intelligently regulating and controlling indexes such as temperature, ventilation and pH value by equipment, so that the ecological balance of compound microorganisms of the microbial fermented feed in the dynamic fermentation process can be realized, and the cost of throwing the strains in each fermentation is saved; the dynamic fermentation is beneficial to producing stable and consistent solid microbial fermentation feed, improving the effective viable count and activity of microorganisms, increasing the content of crude protein, reducing the content of crude fiber, degrading macromolecular substances such as polysaccharide, protein and fat in the feed, producing small molecular substances such as organic acid, polypeptide and amino acid, producing antibacterial substances such as polypeptide, subtilisin and lipid substances, and improving the quality of the solid microbial fermentation feed and antibiotic substitution.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of a drum-type dynamic fermented feed reactor 8 according to the present invention;
FIG. 3 is a schematic view of the front end fixed circular cap 85 of the present invention;
FIG. 4 is a schematic view of the construction of the rear end stationary circular cap 86 of the present invention;
FIG. 5 is a graph showing experimental results of temperature changes at the continuous inlet/outlet after the feed is equilibrated by aerobic fermentation in the drum of embodiment 1 of the present invention;
FIG. 6 is a graph showing experimental results of temperature changes at the continuous inlet/outlet after the feed is equilibrated by aerobic fermentation in the drum in example 2 of the present invention.
Description of reference numerals: a raw material hopper 1, a first conveying mechanism 2, a conveyor belt 21, a second conveying mechanism 22, a third conveying mechanism 23, a stirrer 3, a sterilization tank 4, an exhaust gas output pipeline 41, a heat feedback pipeline 42, an exhaust gas recovery pipeline 43, a first material output pipeline 44, a water pipe 46, a steam generator 5, a steam pipeline 51, a radiator 6, an exhaust gas processor 7, a roller dynamic fermentation feed reactor 8, a temperature sensor 84, a front end fixed circular sealing cap 85, a feed hopper 851, an air inlet input pipeline 852, a first fan 853, a roller outer layer heating and heat preservation system 854, a rear end fixed circular sealing cap 86, a guide plate 861, an exhaust gas output pipeline 862, a second fan 863, a connecting rod 864, a discharge hopper 865, a discharge baffle 866, a driving wheel 87, a driving shaft 88, a driving motor 89, an extrusion granulator 9, a central control system 91, a screen 10, a packing machine 11, a powder 12 recoverer, A one-way valve 13.
Detailed Description
Example 1:
referring to fig. 1 to 4, the technical solution adopted by the present embodiment is: a method for producing solid microbial fermented feed by roller fermentation is characterized by comprising the following steps:
respectively mixing 47 parts of rice bran, 10 parts of vinasse, 10 parts of sweet potato residues, 12 parts of bran, 10 parts of bean pulp, 10 parts of corn flour and 1 part of medium trace elements uniformly by a stirrer 3 of a first conveying mechanism 2 according to a weight dry basis ratio, adding water through a water pipe 46 to ensure that the water content of the material is about 50%, and putting the material into a sterilization container for normal-pressure steam sterilization by circulating steam at 100 ℃ for 8-12 hours, wherein the medium trace elements are calcium carbonate, calcium superphosphate, sodium chloride, magnesium sulfate, zinc sulfate, borax, copper sulfate, manganese sulfate, potassium iodide and ferrous sulfate according to a mass ratio of 5: 5: 3: 1: 0.5: 0.5: 0.5: 0.1: 0.1: 0.1, mixing.
And step two, sterilizing and cooling the raw materials to below 35 ℃, conveying the raw materials into a roller dynamic fermentation feed reactor 8 through a first material output pipeline 44, simultaneously adding 0.5 percent of effective viable bacteria 2 hundred million cfu/g microbial agent into the raw materials, firstly dissolving the strains into a solution barrel, stirring and mixing the strains, conveying the mixed strains to a feed hopper 851 through a water pump, and mixing the mixed strains with the materials input into the feed hopper 851, wherein the carbon-nitrogen ratio of the fermentation materials is 18-25: 1, the water content is about 50%, and the microbial agent 1 is bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens according to the effective viable bacteria ratio of 4: 2: 2, mixing uniformly for later use, wherein the strains are strains for commercial feed.
Thirdly, the central control system 91 is utilized to adjust index parameters such as the rotating speed, the ventilation temperature, the strength, the heat feedback and the like of the dynamic fermentation feed reactor 8 of the roller to keep the dynamic balance fermentation state, the temperature of the fermentation feed which is 0.5 cm away from the feed inlet is about 37 ℃, the temperature of the fermentation feed which is 0.5 cm away from the discharge outlet is about 48 ℃, the fermentation feed which is 70 to 80 percent of the volume of the roller is required to be fed for the first time, the roller is continuously rolled for dynamic fermentation for 7 days, then the materials are discharged according to the fermentation condition (namely, the materials are simultaneously fed and discharged, and the materials can be discharged after being rolled and fermented for seven days in the roller), then about 1/7 of the whole roller material is fed every day, about 1/7 of the whole roller material is discharged every day, the materials are mixed and pushed by the rolling of the roller, and the dynamic balance of the materials of the roller dynamic fermentation feed reactor is kept.
And step four, when discharging every day, opening a baffle of the roller dynamic fermented feed reactor 8 and a discharging conveying device through the central control system 91 to output materials to obtain fermented microbial feed, feeding live pigs 5% of the fermented microbial feed to other complete feeds of the pigs after relevant indexes are qualified through inspection, or adding 25% of the fermented microbial feed mixed dry feed 75% of the fermented microbial feed mixed dry feed into bacillus microbial pellet complete feed through granulation by a granulator 9, and selling and feeding the broiler chickens after the relevant indexes are qualified through inspection. The dry feed comprises the following components: 45 parts of rice bran, 20 parts of corn flour, 14 parts of soybean meal, 8 parts of bran, 5 parts of barley, 3 parts of fish meal and 2 parts of worm powder, 2 parts of bone meal, 1 part of sodium bicarbonate (baking soda), 0.5 part of lysine, 0.5 part of methionine, 0.2 part of cystine, 0.2 part of tryptophan, 0.05 part of vitamin A, 0.05 part of vitamin D and 0.05 part of vitamin E.
The utility model provides an equipment of solid microorganism fermentation feed is produced in cylinder fermentation which characterized in that: the device comprises a raw material hopper 1, a conveying mechanism 2, a stirrer 3, a sterilization tank 4, a steam generator 5, a radiator 6, a waste gas processor 7, a roller dynamic fermentation feed reactor 8, an extrusion granulator 9, a screen 10, a packaging machine 11 and a powder recoverer 12, wherein the bottom of the raw material hopper 1 is provided with the first conveying mechanism 2, the first conveying mechanism 2 is provided with a conveying belt 21, the output end of the first conveying mechanism 2 is provided with the stirrer 3, the stirrer 3 is connected with the sterilization tank 4 through a second conveying mechanism 22, the stirrer 3 is connected with the extrusion granulator 9 through a third conveying mechanism 23, the bottom of the sterilization tank 4 is provided with the steam generator 5, the sterilization tank 4 is provided with a waste gas output pipeline 41, the steam generator 5 is provided with a steam pipeline 51 connected with the waste gas output pipeline 41, the output end of the waste gas output pipeline 41 is connected with a radiator 6, the output end of the radiator 6 is provided with two ends, one end is connected with a waste gas processor 7, the other end is connected with a roller dynamic fermentation feed reactor 8 through a heat feedback pipeline 42, the waste gas output pipeline 41 is also provided with a waste gas recovery pipeline 43, the output end of the waste gas recovery pipeline 43 is connected with the roller dynamic fermentation feed reactor 8, a first material output pipeline 44 is arranged between the sterilization tank 4 and the roller dynamic fermentation feed reactor 8, the upper part of the sterilization tank 4 is provided with a water pipe 46, the roller dynamic fermentation feed reactor 8 is connected with an extrusion granulator 9, the right side of the extrusion granulator 9 is connected with a powder recoverer 12, and the joint between the extrusion granulator 9 and the powder recoverer 12 is provided with a packing machine 11, a screen mesh 10 is arranged above the packaging machine 11.
Furthermore, the steam pipeline 51, the exhaust gas output pipeline 41 and the exhaust gas recovery pipeline 43 are provided with check valves 13.
Further, a temperature sensor 84 with a plurality of point positions is arranged on the roller dynamic fermentation feed reactor 8, one end of the roller dynamic fermentation feed reactor 8 is provided with a front end fixed circular sealing cap 85, the other end of the roller dynamic fermentation feed reactor is provided with a rear end fixed circular sealing cap 86, the front end fixed circular sealing cap 85 is provided with a feed hopper 851, the side of the feed hopper 851 is provided with an air inlet input pipeline 852 and a first fan 853, the air inlet input pipeline 852 is connected with the first fan 853, the periphery of the roller dynamic fermentation feed reactor 8 is provided with a roller outer layer heating and heat insulating system 854, one side of the rear end fixed circular sealing cap 86 is provided with a guide plate 861, the other side of the rear end fixed circular sealing cap is provided with an exhaust gas output pipeline 862 and a second fan 863, the exhaust gas output pipeline 862 is connected with the second fan 863, the bottom of the exhaust gas output pipeline 862 is connected with a discharge hopper 865 through a connecting rod 864, a discharge baffle 866 is arranged on the discharge hopper 865, a pair of driving wheels 87 is arranged at the bottom of the roller dynamic fermentation feed reactor 8, a driving shaft 88 is connected between the driving wheels 87, and a driving motor 89 is arranged at the middle bottom of the driving shaft 88.
Further, temperature-sensing ware 84 can know the fermentation temperature in real time, stock guide 861 be used for guiding the ejection of compact, outer heating heat preservation system 854 of cylinder can carry out temperature intelligent control to the fermentation.
Furthermore, the outside of the device for producing the solid microbial fermented feed through the roller fermentation is electrically connected with a central control system 91, the central control system 91 is connected with all components, the normal operation of all the components is monitored through the temperature sensor 84 and the air management system, and relevant data parameters are adjusted to control the fermentation efficiency.
Further, after sterilizing the fermentation raw materials in this example, microbial feeds were produced by different fermentation methods: CK, solid submerged fermentation feed (fermentation at 120 cm high pile); and T, performing roller aerobic fermentation on the feed (fermentation is performed in the step of example 1), adding 2 hundred million cfu/g of microbial agent (wherein the ratio of effective viable bacteria of the bacillus subtilis, the bacillus licheniformis and the bacillus amyloliquefaciens is 4: 2: 2) of 0.5% of raw materials, performing two fermentation periods of 7 days, recording the temperatures of different fermentation modes every day, wherein CK is the temperature of a compost at a depth of 50 cm, and T is the temperature of the inner wall of a roller. After fermenting for 7 days, taking fermentation material samples of two different fermentation modes, and measuring the quantity of bacillus, mould, water content, aflatoxin B1, ochratoxin A, crude protein and crude fiber content. The total bacillus number determination method comprises the following steps: 5g of fermentation sample is added into 50mL of sterile water, after water bath at 80 ℃ is adopted for 20 minutes, the measurement of other indexes which are not particularly described is carried out by referring to related national standards and other methods, and further, the test result of the temperature change of the continuous feed inlet and the continuous feed outlet after the aerobic fermentation feed of the method and the equipment for producing the solid microbial fermentation feed by roller fermentation is balanced can refer to fig. 5.
Further, after the balance of the roller aerobic fermented feed, the temperature of a feeding port is basically maintained to be about 37 ℃, the temperature of a discharging port is basically maintained to be about 48 ℃, the dynamic balance is maintained, the fastest propagation speed of bacillus in the fermented feed is kept near the feeding port, the spores are gradually formed near the discharging port under the high-temperature condition, and the solid submerged fermented feed under the condition of the same raw material proportion is slow in starting due to early fermentation temperature, slow in propagation speed when the fermentation of the bacillus is started, high in temperature rise during later fermentation and higher than 60 ℃, so that the propagation of the bacillus in the solid submerged fermented feed is inhibited.
Figure RE-167793DEST_PATH_IMAGE001
TABLE 1 Effect of different treatment regimes on the number of microorganisms in feed
Note: different lower case letters in the same column indicate significant differences between the different treatments at the same time (P < 0.05), as follows.
As can be seen from Table 1, the number of Bacillus after 7 days of roller aerobic fermentation of the feed is significantly higher than that of solid submerged fermentation feed, while the number of mould and aflatoxin B is significantly higher1And ochratoxin A is obviously lower than that of solid submerged fermentation feed. The method is mainly characterized in that the roller aerobic fermentation feed is dynamically fermented in a closed roller, the continuous feeding and continuous discharging are carried out, microorganisms in the middle section of the roller are basically rapidly propagated, high-density bacillus accounts for dominant flora and generates polypeptide, lipid antibacterial substances and the like for inhibiting the propagation of fungi, the propagation of the fungi is difficult, the quantity of the fungi is small, the bacillus can generate enzymes for decomposing aflatoxin and ochratoxin to cause low content of the enzymes, the initial temperature of the temperature rising stage of the solid submerged fermentation feed is low, the growth and propagation of the fungi are facilitated, the content of the fungi is obviously higher than that of the roller aerobic fermentation feed, and the bacillus forms spores without mass propagation under the subsequent high-temperature condition. Therefore, the fermentation efficiency of the roller aerobic fermentation feed is obviously faster than that of the solid submerged fermentation feed, and the concentration of the bacillus is higher.
Figure RE-RE-DEST_PATH_IMAGE003
TABLE 2 Effect of different treatment modalities on the crude protein and crude fiber content of fermented feed
As can be seen from Table 2, after 7 days of the drum aerobic fermentation feed and 7 days of the solid submerged fermentation feed, the water content and the crude protein content of the drum aerobic fermentation feed are obviously higher than those of the solid submerged fermentation feed, while the crude fiber content is obviously lower than those of the solid submerged fermentation feed, which indicates that the drum aerobic fermentation feed has the advantages of fast microbial propagation, fast temperature rise, large evaporation capacity and obviously lower water content of the materials, and the drum aerobic fermentation feed is beneficial to improving the crude protein content in the feed, reducing the crude fiber content and improving the quality of the fermented feed.
Example 2:
the method for producing the solid microbial fermented feed by drum dynamic fermentation comprises the following operation steps:
step one, respectively mixing 42 parts of corn straws, 18 parts of corncobs, 16 parts of edible fungus residues, 8 parts of bran, 8 parts of cottonseed meal, 6 parts of corn flour, 2 parts of cane molasses and 0.5 part of medium trace elements uniformly by a stirrer 3 of a first conveying mechanism 2 according to a weight dry basis ratio, adding water through a water pipe 46 to ensure that the water content of the materials is about 55%, putting the materials into a sterilization container to perform normal-pressure intermittent steam sterilization (after 100 ℃ circulating steam is used for 1 hour and then placed at 20-25 ℃ for 24 hours or is kept overnight at normal temperature, sterilizing by the previous conditions, repeating the steps for three times), wherein the medium trace elements are calcium carbonate, calcium superphosphate, sodium chloride, magnesium sulfate, zinc sulfate, borax, copper sulfate, manganese sulfate, potassium iodide and ferrous sulfate according to a mass ratio of 2: 2: 2: 0.5: 0.5: 0.5: 0.5: 0.1: 0.1: 0.1, mixing.
And step two, sterilizing and cooling the raw materials to below 35 ℃, conveying the raw materials into a roller dynamic fermentation feed reactor 8 through a first material output pipeline 44, simultaneously adding 0.25 percent of effective viable bacteria 2 hundred million cfu/g microbial agent, dissolving the strains into a solution barrel, stirring and mixing the strains, conveying the mixed strains to a feed hopper 851 through a water pump, mixing the mixed strains with the material input into the feed hopper 851, wherein the carbon-nitrogen ratio of the fermentation material is 25-30: 1, the water content is about 55%, and the microbial agent is bacillus subtilis, bacillus licheniformis, candida utilis, saccharomyces cerevisiae, aspergillus niger, aspergillus oryzae and white rot fungi, wherein the effective viable bacteria ratio is 1: 1: 1: 1: 1: 1: 1, uniformly mixing the strains for later use, wherein the strains are strains for commercial feed.
Thirdly, the central control system 91 is utilized to adjust index parameters such as the rotating speed, the ventilation temperature, the strength, the heat feedback and the like of the dynamic fermentation feed reactor 8 of the roller to keep the dynamic balance fermentation state, the temperature of the fermentation feed which is 0.5 cm away from the feed inlet is about 35 ℃, the temperature of the fermentation feed which is 0.5 cm away from the discharge outlet is about 45 ℃, the fermentation feed which is 70 to 80 percent of the volume of the roller is required to be fed for the first time, the roller is continuously rolled for dynamic fermentation for 7 days, then the materials are discharged according to the fermentation condition (namely, the materials are simultaneously fed and discharged, and the materials can be discharged after being rolled and fermented for seven days in the roller), then about 1/7 of the whole roller material is fed every day, about 1/7 of the whole roller material is discharged every day, the materials are mixed and pushed by the rolling of the roller, and the dynamic balance of the materials of the roller dynamic fermentation feed reactor is kept.
And step four, when discharging every day, opening a baffle of the roller dynamic fermented feed reactor 8 and discharging conveying equipment through the central control system 91 to output materials to obtain fermented microbial feed, and directly feeding beef cattle or adding 10% of fermented microbial feed to mix with other coarse feed after relevant indexes are qualified through inspection.
Further, the fermentation raw materials in this example were sterilized and then compared by different microbial feed fermentation methods: CK, solid submerged fermentation feed (for heap fermentation with a thickness of 50 cm when tiled); and T, performing roller aerobic fermentation on the feed (fermentation is performed in the step of the embodiment 2), and simultaneously adding 0.25% of 2 hundred million cfu/g of microbial agent (wherein the ratio of effective viable bacteria of the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, the aspergillus niger, the aspergillus oryzae and the white rot fungi is 1: 1: 1: 1) during CK treatment. The fermentation period of the two fermentation modes is 7 days, the temperature of different fermentation modes is recorded every day, CK is the temperature of a treated pile body in a depth of 25 cm, and T is the temperature of the inner wall of the roller. After fermenting for 7 days, taking samples of two different fermentation modes, and measuring the quantity of bacillus and saccharomyces cerevisiae and the content of crude protein and crude fiber. The total bacillus number determination method comprises the following steps: 5g of fermentation sample is added into 50mL of sterile water, after water bath at 80 ℃ is adopted for 20 minutes, the mixture is subjected to a gradient dilution plating culture counting method, and other indexes which are not particularly described are determined by referring to related national standards and other methods.
Further, referring to fig. 6, the temperature variation test results of the continuous feeding and discharging port after the aerobic fermentation feed of the method and the device for producing the solid microbial fermentation feed by roller fermentation can be seen, the temperature of the feeding port of the roller fermentation reactor is basically maintained at about 35 ℃, the temperature of the discharging port is basically maintained at about 45 ℃, the dynamic balance is maintained under the condition of the temperature range, the propagation speed of the microbes in the roller fermentation reactor is high, the fermentation temperature of the solid submerged fermentation feed under the condition of the same raw material ratio is slowly started, and the propagation of the microbes is slow.
Figure RE-573629DEST_PATH_IMAGE004
TABLE 1 Effect of different treatment regimes on the number of microorganisms in feed
As can be seen from Table 1, after 7 days of the roller aerobic fermentation feed, the number of bacillus and saccharomyces cerevisiae is significantly higher than that of the solid submerged fermentation feed, and the number of aflatoxin B1 and ochratoxin A are significantly lower than that of the solid submerged fermentation feed. Mainly because the drum aerobic fermentation feed is in closed drum dynamic fermentation, because of continuous feeding and continuous discharging, the temperature change of the whole drum is small, the microorganisms are basically in rapid propagation, while the initial temperature rise of the solid submerged fermentation feed is slow, the microorganism propagation is slow, and the concentration of bacillus and saccharomyces cerevisiae is relatively low. Meanwhile, the active compound microorganisms in the roller can generate more enzymes for decomposing aflatoxin B1 and ochratoxin A, so that the content of the aflatoxin B1 and the ochratoxin A is low. Therefore, the fermentation efficiency of the roller aerobic fermentation feed is obviously faster than that of the solid submerged fermentation feed.
Treatment of Water content (%) Crude protein content (%) Crude fiber content (%)
Cylinder aerobic fermentation feed (T) 50.22b 12.42a 18.12b
Solid submerged fermentation feed (CK) 53.07a 10.89b 21.16a
TABLE 2 Effect of different treatment modalities on the crude protein and crude fiber content of fermented feed
As can be seen from Table 2, after the feed is aerobically fermented for 7 days in the drum, and after the feed is deeply fermented for 7 days in the solid state, the crude protein content of the aerobically fermented feed in the drum is obviously higher than that of the solid state, while the water content and crude fiber content are obviously lower than that of the solid state, which indicates that the aerobically fermented feed in the drum has the advantages of fast microbial propagation, fast temperature rise, large evaporation capacity and obviously lower water content of the material, and the aerobically fermented feed in the drum is beneficial to improving the crude protein content in the feed, reducing the crude fiber content and improving the quality of the fermented feed.
After the technical scheme is adopted, the invention has the beneficial effects that: the solid microbial fermentation feed can be produced by utilizing crop straws such as rice straws, corns and the like, is beneficial to high-value utilization of the feed of crop straw resources, and promotes the sustainable development of circular agriculture; the horizontal roller is a dynamic fermentation process for simultaneously feeding and discharging, so that the continuous production of microbial fermented feed is realized, and the efficiency is high; the horizontal roller can effectively and quickly propagate and occupy the advantages only by throwing the microbial strains once, and the growth and propagation of the microbial strains are stabilized by scientifically preparing raw materials and intelligently regulating and controlling indexes such as temperature, ventilation and pH value by equipment, so that the ecological balance of compound microorganisms of the microbial fermented feed in the dynamic fermentation process can be realized, and the cost of throwing the strains in each fermentation is saved; the dynamic fermentation is beneficial to producing stable and consistent solid microbial fermentation feed, improving the effective viable count and activity of microorganisms, increasing the content of crude protein, reducing the content of crude fiber, degrading macromolecular substances such as polysaccharide, protein and fat in the feed, producing small molecular substances such as organic acid, polypeptide and amino acid, producing antibacterial substances such as polypeptide, subtilisin and lipid substances, and improving the quality of the solid microbial fermentation feed and antibiotic substitution.
The above description is only for the purpose of illustrating the technical solutions of the present invention and not for the purpose of limiting the same, and other modifications or equivalent substitutions made by those skilled in the art to the technical solutions of the present invention should be covered within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims (6)

1. A method for producing solid microbial fermented feed by roller fermentation is characterized by comprising the following steps:
respectively mixing 15-90 parts of straw powder and/or crushed chaff and/or rice bran and/or corn straw and/or corn cob and/or wheat straw and other crop straws, 2-25 parts of edible fungus residues and/or sweet potato residues and/or cassava residues and/or bean residues and/or kudzu vine residues and/or vinasse and/or potato residues and/or fruit residues and other agricultural and sideline processing residues, 2-25 parts of bran and/or corn bran and/or soybean hull and/or corn germ meal, 0.5-25 parts of soybean meal and/or peanut meal and/or cotton seed meal and/or rapeseed meal and other plant source protein raw materials, 0.5-15 parts of corn meal and/or corn molasses and/or soybean molasses and/or cane molasses and/or fermented molasses and/or glucose, 0.1-5 parts of medium trace elements are respectively loaded into different raw material hoppers (1), the raw materials are uniformly mixed by a stirrer (3) of a first conveying mechanism (2), water is added through a water pipe (46) to ensure that the water content of the materials is 45-60%, the materials are input into a sterilization container (4) to be subjected to normal-pressure steam sterilization (8-12 hours by adopting 100 ℃ circulating steam) or normal-pressure intermittent steam sterilization (1 hour by using 100 ℃ circulating steam and then placed at 20-25 ℃ for storage for 24 hours or overnight at normal temperature, the sterilization is carried out under the previous conditions, and the steps are repeated for three times), wherein the medium trace elements are calcium carbonate, calcium superphosphate, sodium chloride, magnesium sulfate, zinc sulfate, borax, copper sulfate, manganese sulfate, potassium iodide and ferrous sulfate according to the mass ratio of 0.2-15: 0.2-10: 0.2-10: 0.1-10: 0.1-5: 0.1-5: 0.1-4: 0-2: 0-2: 0 to 2, and mixing.
And secondly, sterilizing and cooling the raw materials to be below 35 ℃, conveying the raw materials into a roller dynamic fermentation feed reactor (8) through a first material output pipeline (44), simultaneously adding 0.1-2.0% of microbial agent, feeding the fermentation material which is 70-80% of the volume of the roller for the first time, designing the frequency of the roller according to the process requirement of feed fermentation, continuously rolling the roller dynamic fermentation feed reactor (8), discharging the materials according to the fermentation condition after 3-10 days, feeding and discharging the materials every day according to the fermentation period, mixing and propelling the materials through the rolling of the roller, and keeping the dynamic balance of the materials of the roller dynamic fermentation feed reactor. The microbial agent is bacillus subtilis, bacillus licheniformis, bacillus amyloliquefaciens, bacillus coagulans, bacillus natto, bacillus cereus, bacillus pumilus, candida utilis, saccharomyces cerevisiae, rhizopus, aspergillus niger, aspergillus oryzae and white rot fungi, and the ratio of effective viable bacteria is 0-10: 0-10: 0-10: 0-8: 0-8: 0-8: 0-8: 0-8: 0-8: 0-5: 0-5: 0-5: 0-5, and mixing uniformly for later use, wherein the strains are strains for commercial feed.
And step three, a central control system (91) is utilized to adjust index parameters such as the rotating speed, the ventilation temperature, the strength and the heat feedback of the roller dynamic fermentation feed reactor 8 to keep the dynamic balance fermentation state, the temperature of the fermentation feed which is 0.5 cm away from a feed inlet is about 30-52 ℃ (the specific temperature is determined according to the characteristics of matched roller equipment, strains and fermentation products), and the temperature of the fermentation feed which is 0.5 cm away from a discharge outlet is about 35-60 ℃ (the specific temperature is determined according to the characteristics of matched roller equipment, strains and fermentation products).
Step four, when discharging every day, opening a baffle of the roller dynamic fermented feed reactor (8) and a discharging conveying device through a central control system (91) to output materials to obtain fermented microbial feed, adding 0.5-10% (calculated according to dry basis, the same below) of fermented microbial feed into other mixed feeds to feed livestock (the placing time is not more than 15 days) or taking 5% -30% of fermented feed to mix with other dry feed extrusion granulators (9) to granulate and then feeding livestock and poultry, wherein the dry feed is: 10-75 parts of rice bran, 8-55 parts of corn flour, 5-30 parts of soybean meal, 2-20 parts of bran, 0-15 parts of barley, 0.5-10 parts of fish meal and/or earthworm powder and/or insect powder, 0-5 parts of bone meal, 0-2 parts of sodium bicarbonate (baking soda), 0-1 part of lysine, 0-0.5 part of methionine, 0-0.5 part of cystine, 0-0.5 part of tryptophan, 0-0.1 part of vitamin A, 0-0.1 part of vitamin D and 0-0.1 part of vitamin E.
2. The apparatus for producing solid microbial fermented feed by drum fermentation according to claim 1, wherein: the device comprises a raw material hopper (1), a first conveying mechanism (2), a stirrer (3), a sterilization tank (4), a steam generator (5), a radiator (6), an exhaust gas processor (7), a roller dynamic fermentation feed reactor (8), an extrusion granulator (9), a screen (10), a packaging machine (11) and a powder recoverer (12), wherein the first conveying mechanism (2) is arranged at the bottom of the raw material hopper (1), a conveying belt (21) is arranged on the first conveying mechanism (2), the stirrer (3) is arranged at the output end of the first conveying mechanism (2), the stirrer (3) is connected with the sterilization tank (4) through a second conveying mechanism (22), the stirrer (3) is connected with the extrusion granulator (9) through a third conveying mechanism (23), and the steam generator (5) is arranged at the bottom of the sterilization tank (4), the device is characterized in that a waste gas output pipeline (41) is arranged on the sterilization tank (4), a steam pipeline (51) connected with the waste gas output pipeline (41) is arranged on the steam generator (5), the output end of the waste gas output pipeline (41) is connected with a radiator (6), the output end of the radiator (6) is provided with two ends, one end of the radiator is connected with a waste gas processor (7), the other end of the radiator is connected with a roller dynamic fermentation feed reactor (8) through a heat feedback pipeline (42), a waste gas recovery pipeline (43) is further arranged on the waste gas output pipeline (41), the output end of the waste gas recovery pipeline (43) is connected with the roller dynamic fermentation feed reactor (8), a first material output pipeline (44) is arranged between the sterilization tank (4) and the roller dynamic fermentation feed reactor (8), a water pipe (46) is arranged at the upper part of the sterilization tank (4), the dynamic fermented feed reactor with the rotary drum (8) is connected with an extrusion granulator (9), the right side of the extrusion granulator (9) is connected with a powder recoverer (12), a packing machine (11) is arranged at the joint between the extrusion granulator (9) and the powder recoverer (12), and a screen (10) is arranged above the packing machine (11).
3. The apparatus for producing solid microbial fermented feed by drum fermentation according to claim 2, wherein: and the steam pipeline (51), the waste gas output pipeline (41) and the waste gas recovery pipeline (43) are provided with one-way valves (13).
4. The apparatus for producing solid microbial fermented feed by drum fermentation according to claim 2, wherein: the device is characterized in that a temperature sensor (84) with a plurality of point positions is arranged on the roller dynamic fermentation feed reactor (8), one end of the roller dynamic fermentation feed reactor (8) is provided with a front end fixed circular sealing cap (85), the other end of the roller dynamic fermentation feed reactor is provided with a rear end fixed circular sealing cap (86), the front end fixed circular sealing cap (85) is provided with a feed hopper (851), the side of the feed hopper (851) is provided with an air inlet input pipeline (852) and a first fan (853), the air inlet input pipeline (852) is connected with the first fan (853), the periphery of the roller dynamic fermentation feed reactor (8) is provided with a roller outer layer heating and heat preservation system (854), one side of the rear end fixed circular sealing cap (86) is provided with a guide plate (861), and the other side is provided with a waste gas output pipeline (862) and a second fan (863), the waste gas output pipeline (862) is connected with a second fan (863), the bottom of the waste gas output pipeline (862) is connected with a discharge hopper (865) through a connecting rod (864), a discharge baffle (866) is arranged on the discharge hopper (865), the bottom of the roller dynamic fermentation feed reactor (8) is provided with a pair of driving wheels (87), a driving shaft (88) is connected between the driving wheels (87), and the bottom of the middle of the driving shaft (88) is provided with a driving motor (89).
5. The apparatus for producing solid microbial fermented feed by drum fermentation according to claim 4, wherein: temperature-sensing ware (84) can know fermentation temperature in real time, stock guide (861) be used for guiding the ejection of compact, outer heating heat preservation system of cylinder (854) can carry out temperature intelligent control to the fermentation.
6. The apparatus for producing solid microbial fermented feed by drum fermentation according to claim 2, wherein: the device is electrically connected with a central control system (91) outside, the central control system (91) is connected with all components, monitors the normal operation of all the components through a temperature sensor (84) and an air management system, and adjusts related data parameters to control the fermentation efficiency.
CN202110206399.9A 2021-02-24 2021-02-24 Method and equipment for producing solid microbial fermented feed by roller fermentation Active CN113229397B (en)

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CN115886134A (en) * 2022-11-28 2023-04-04 武汉轻工大学 Feed preparation system using bean dregs as raw materials

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