CN113197809B - Preparation method of skin care product containing phaeodactylum and tara fermentation compound - Google Patents
Preparation method of skin care product containing phaeodactylum and tara fermentation compound Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a preparation method of a skin care product containing a phaeodactylum and tara fermentation compound, which comprises the following steps: s1, preparing Phaeodactylum algae and tara fermentation liquor, adding tara pod extract into glycerol aqueous solution, inoculating lactobacillus for fermentation conversion reaction, adding Phaeodactylum tricornutum extract, inoculating lactobacillus for fermentation selective esterification reaction to obtain fermentation liquor; s2, preparing a Phaeodactylum algae and tara fermentation compound, filtering or centrifuging the fermentation liquor, homogenizing and sterilizing the filtrate or the centrifugal supernatant to obtain the Phaeodactylum algae and tara fermentation compound; s3, preparing the skin care product containing the phaeodactylum algae and tara fermentation compound. The preparation method provided by the invention can be used for preparing the phaeodactylum and tara fermentation compound and taking the phaeodactylum and tara fermentation compound as the raw materials of the skin care product, so that the prepared skin care product has double synergistic effects in the molecule to effectively reduce the protein carbonylation level in the skin, repair the skin and improve the skin glossiness in two ways of reducing the ROS content and activating the 20S proteasome.
Description
[ technical field ] A method for producing a semiconductor device
The invention belongs to the technical field of skin care products, and particularly relates to a preparation method of a skin care product containing a phaeodactylum algae and tara fermentation compound.
[ background of the invention ]
According to statistics, 77% of people around the world suffer from physiological diseases caused by stress, and the stress is mainly caused by work strain, examination and research, environmental pollution and the like. Long-term pressure causes the water content of the skin to decrease, the percutaneous dehydration to increase, and the skin shows dark yellow and dull.
The cause of skin problems due to stress is protein carbonylation in the skin, which is formed by the reaction of amino groups in proteins with reactive aldehyde compounds generated by lipid peroxidation caused by Reactive Oxygen Species (ROS). The accumulation of the carbonylated proteins over time, and the difficulty of degradation, may result in an overburdened cellular repair/degradation system, resulting in the accumulation of more carbonylated proteins, which gradually gives rise to a dull yellow and dull skin.
The traditional perception of dark yellow skin has a false area: on the one hand, dark yellow skin is considered as the front-end characteristic expression of skin darkness. The two mechanisms are different from each other: skin black is associated with melanin in the skin: melanin is produced by the melanosomes of melanocytes. According to skin physiology, the main factor for determining skin color depth is skin melanin content and distribution. Melanocytes are located in the basal layer of skin, and melanin secretion by melanocytes is an extremely complex biological process, and is related to mechanisms such as generation, dendrite formation, melanosome maturation and transportation of melanocytes, and is closely related to keratinocytes, extracellular matrix and the like. On the other hand, it is thought that the skin dark yellow is caused by protein glycosylation: glycosylation of proteins is the binding of the carbonyl group of a reducing sugar (e.g., glucose or fructose) to a protein in a non-enzymatic reaction to form a glycated protein. However, the skin yellowing effect caused by protein carbonylation reaction is much higher than that caused by skin saccharification reaction.
The skin care product obtained by the existing skin care product preparation method cannot effectively reduce the occurrence of protein carbonylation reaction, so that the skin care effect is poor.
[ summary of the invention ]
The invention aims to solve the problems in the background art and provides a preparation method of a skin care product containing a phaeodactylum algae and tara fermentation compound.
In order to achieve the purpose, the invention provides a preparation method of a skin care product containing a phaeodactylum and tara fermentation compound, which comprises the following steps:
s1, preparing Phaeodactylum algae and tara fermentation liquor, adding a tara pod extract into a glycerol aqueous solution, inoculating lactic acid bacteria, performing fermentation conversion reaction at the temperature of 20-40 ℃, adding a Phaeodactylum tricornutum extract, inoculating the lactic acid bacteria, and performing fermentation selective esterification reaction at the temperature of 35-50 ℃ to obtain the fermentation liquor;
s2, preparing a Phaeodactylum algae and tara fermentation compound, filtering or centrifuging the fermentation liquor, homogenizing and sterilizing the filtrate or the centrifugal supernatant to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing a skin care product, namely selecting a part of skin care product raw materials as a phase A to be heated and uniformly mixed, selecting another part of skin care product raw materials as a phase B to be heated and uniformly mixed, then adding the phase B into the phase A or adding the phase A into the phase B to be subjected to homogeneous emulsification treatment, adding the phaeodactylum algae and tara fermentation compound to be subjected to homogeneous emulsification treatment after cooling, and finally adding hydrolat and stirring to obtain the skin care product;
the skin care product is essence or cream, and the skin care product comprises a plurality of raw materials of water, a humectant, a thickening agent, an emollient, an emulsifier and an antioxidant.
Preferably, the lactic acid bacteria comprise one or more of lactobacillus casei, lactobacillus crispatus, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus formaticus, lactobacillus helveticus, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius.
Preferably, the concentration of the lactic acid bacteria participating in the reaction is 1X 109～9×1011CFU/mL, the inoculation amount of the lactobacillus is the volume of the solution, and the reaction time of the lactobacillus is 1-5 days.
Preferably, the tara pod extract is dissolved in a glycerol aqueous solution with the mass fraction of 30-50%, and the mass ratio of the tara pod extract to the glycerol aqueous solution is 1: 19-99.
Preferably, the mass ratio of the phaeodactylum tricornutum extract to the tara pod extract is 1.5-5.
Preferably, the emollient comprises dimethicone, ethylhexyl hydrogenated olive oleate, unsaponifiable hydrogenated olive oil, isononyl isononanoate, cyclopentadimethicone, cyclohexasiloxane, PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, caprylic/capric triglyceride, cocoa butter and petrolatum, the moisturizer comprises one or more of polyethylene glycol-32, glyceryl polyether-26, propylene glycol, allantoin, 1, 2-hexanediol, octylhydroximic acid, 1, 2-pentanediol, arginine, the thickener comprises one or more of sodium polyacryl dimethyltaurate, xanthan gum, stearic acid, the emulsifier comprises tris (laureth-4) phosphate, glyceryl stearate, sodium lauryl sulfate, and sodium lauryl sulfate, One or more of PEG-100 stearate, behenyl alcohol and cetearyl ester.
Preferably, the antioxidant comprises one or more of tocopherol acetate, ascorbyl stearate, ascorbyl palmitate.
Preferably, the hydrolat comprises one or more of neroli floral water, rose floral water, egyptian blue water lily floral water, white chrysanthemum flower water, hamamelis water, golden chamomile floral water and plum blossom floral water.
Preferably, the formula of the essence comprises the following components in parts by weight: 53-86 parts of water, 5-10 parts of a humectant, 1-3 parts of a thickener, 3-10 parts of an emollient, 1-7 parts of an emulsifier, 2-10 parts of a Phaeodactylum/tara fermentation compound and 2-7 parts of hydrolat.
Preferably, the formula of the face cream comprises the following components in parts by weight: 48-81.5 parts of water, 3-7 parts of a humectant, 1-3 parts of a thickener, 6-13 parts of an emollient, 3-10 parts of an emulsifier, 0.5-2 parts of an antioxidant, 2-10 parts of a phaeodactylum/tara fermentation compound and 3-7 parts of hydrolat.
The invention has the beneficial effects that: according to the invention, the Phaeodactylum tricornutum extract and the tara pod extract are fermented to obtain the Phaeodactylum tricornutum and tara fermentation compound, and the fermentation mixture is used as an addition raw material of the cream or essence, so that the prepared cream or essence can effectively reduce the protein carbonylation level in the skin by dual synergistic action in the molecule to reduce the ROS content and activate the 20S proteasome, repair the skin, improve the skin glossiness and realize multiple skin care effects.
[ detailed description ] embodiments
The technical solution of the present invention is further illustrated by the following examples:
examples 1 to 4 (wherein examples 1 and 2 are methods and formulations for preparing a serum skin care product, and examples 3 and 4 are methods and formulations for preparing a cream skin care product).
Example 1:
a preparation method of a skin care product containing Phaeodactylum algae and tara fermentation compound, in particular to a preparation method of essence containing Phaeodactylum algae and tara fermentation compound, which comprises the following steps:
s1, preparing Phaeodactylum and tara fermentation liquor, adding 1kg of tara pod extract into 50kg of 30% glycerol aqueous solution according to the volume of the solution and the volume of the solution is 1 multiplied by 1010Inoculating Lactobacillus rhamnosus to CFU/mL, fermenting at 20 deg.C for 5 days, adding 2kg Phaeodactylum tricornutum extract, and adding at a volume of 3 × 1010Inoculating lactobacillus casei in CFU/mL, and performing fermentation selective esterification at 35 ℃ for 5 days to obtain fermentation liquor;
s2, preparing a Phaeodactylum algae and tara fermentation compound, filtering the fermentation liquor obtained in the step 1, and homogenizing and sterilizing the filtrate to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing essence: selecting water, polyethylene glycol-32, glyceryl polyether-26 and xanthan gum as phase A, mixing and heating to 70 deg.C, stirring for 15 min to make the material uniform; selecting polydimethylsiloxane, hydrogenated olive oil ethylhexyl oleate, hydrogenated olive oil unsaponifiable matter, tri (laureth-4) phosphate, glycerol stearate and PEG-100 stearate as a phase B, mixing and heating to 70 ℃ according to a proportion, and stirring for 15 minutes to ensure that the material body is uniform; adding phase B into phase A, homogenizing for 2 min to emulsify; stirring and cooling to 40 ℃, adding the Phaeodactylum algae and Tara fermentation compound in proportion, and homogenizing for 2 minutes until emulsification is uniform; adding rose flower water according to the proportion, and stirring for 15 minutes to ensure that the material body is uniform to obtain the essence.
The formula of the essence applied to the preparation method of the essence is shown in the following table 1:
TABLE 1 formulation table (by weight percentage content)
Example 2:
a preparation method of a skin care product containing Phaeodactylum algae and tara fermentation compound, in particular to a preparation method of essence containing Phaeodactylum algae and tara fermentation compound, which comprises the following steps:
s1, preparing Phaeodactylum algae andtara fermentation broth, adding 1kg of tara pod extract into 19kg of 40% glycerol aqueous solution, and mixing at a volume of 9 × 1011Inoculating Lactobacillus helveticus at CFU/mL, performing fermentation conversion reaction at 30 deg.C for 3 days, adding 5kg Phaeodactylum tricornutum extract, and making into 5 × 10 solution according to volume11Inoculating lactobacillus plantarum in a ratio of CFU/mL, and performing fermentation selective esterification reaction at 40 ℃ for 3 days to obtain a Phaeodactylum algae and tara fermentation compound;
s2, preparing a Phaeodactylum algae and tara fermentation compound, centrifuging the fermentation liquor obtained in the step 1, and homogenizing and sterilizing the supernatant liquor to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing essence: selecting water, 1, 2-hexanediol, octyl hydroximic acid and stearic acid as phase A, mixing and heating to 80 ℃ according to a proportion, and stirring for 30 minutes to ensure that the material body is uniform; selecting PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, behenyl alcohol and cetearyl ester as phase B, mixing and heating to 80 ℃ in proportion, and stirring for 30 minutes to make the material body uniform; adding phase B into phase A, homogenizing for 20 min to emulsify; stirring and cooling to 38 ℃, adding the Phaeodactylum algae and Tara fermentation compound in proportion, and homogenizing for 20 minutes until emulsification is uniform; adding the neroli water in proportion and stirring for 30 minutes to ensure that the material body is uniform to obtain the essence.
The formula of the essence applied to the preparation method of the essence is shown in the following table 2:
TABLE 2 formulation table (by weight percentage content)
Example 3:
a preparation method of a skin care product containing a Phaeodactylum algae and tara fermentation compound, in particular to a preparation method of a cream containing the Phaeodactylum algae and tara fermentation compound, which comprises the following steps:
s1, preparing Phaeodactylum and tara fermentation liquor, adding 1kg of tara pod extract into 99kg of 50% glycerol aqueous solution according to the volume of the solution and the volume of 1 × 109Inoculating Lactobacillus fermentum to CFU/mL, fermenting at 40 deg.C for 1 day, adding 1.5kg Phaeodactylum tricornutum extract, and mixing at a volume of 6 × 109Inoculating lactobacillus crispatus in CFU/mL, and performing fermentation selective esterification reaction at 50 ℃ for 1 day to obtain fermentation liquor;
s2, preparing a Phaeodactylum algae and tara fermentation compound, filtering the fermentation liquor obtained in the step 1, and homogenizing and sterilizing the filtrate to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing the face cream: selecting isononyl isononanoate, cyclopentadimethylsiloxane, cyclohexasiloxane, sodium polyacryloyldimethyltaurate, behenyl alcohol, cetearyl stearate and tocopheryl acetate as phase A, mixing and heating to 85 ℃ in proportion, and stirring for 15 minutes to make the material body uniform; selecting propylene glycol, allantoin, water and xanthan gum as a phase B, mixing and heating to 85 ℃ in proportion, and stirring for 15 minutes to ensure that the material is uniform; adding the phase A into the phase B, and homogenizing for 2 minutes until the emulsion is uniform; stirring and cooling to 30 ℃, adding the Phaeodactylum algae and Tara fermentation compound in proportion, and homogenizing for 2 minutes until emulsification is uniform; adding Egyptian blue water lily flower water according to the proportion, and stirring for 15 minutes to ensure that the material body is uniform to obtain the face cream.
The formula of the face cream applied to the preparation method of the face cream is shown in the following table 3:
TABLE 3 recipe table (by weight percentage)
Example 4:
a preparation method of a skin care product containing a Phaeodactylum algae and tara fermentation compound, in particular to a preparation method of a cream containing the Phaeodactylum algae and tara fermentation compound, which comprises the following steps:
s1, preparing Phaeodactylum and tara fermentation liquor, adding 1kg of tara pod extract into 70kg of 45% glycerol aqueous solution, and performing concentration according to the volume of the solution by 9 x 1010Inoculating Lactobacillus formaticus at CFU/mL, performing fermentation conversion reaction at 35 deg.C for 2 days, adding 3.5kg Phaeodactylum tricornutum extract, and mixing at a volume of 9 × 1010Inoculating lactobacillus crispatus in CFU/mL, and performing fermentation selective esterification reaction for 2 days at 45 ℃ to obtain fermentation liquor;
s2, preparing a Phaeodactylum algae and tara fermentation compound, centrifuging the fermentation liquor obtained in the step 1, and homogenizing and sterilizing the supernatant liquor to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing the face cream: selecting cocoa seed butter, petrolatum, glyceryl stearate, PEG-100 stearate and ascorbyl palmitate as phase A, mixing and heating to 75 ℃ in proportion, and stirring for 30 minutes to ensure that the material is uniform; selecting 1, 2-pentanediol, arginine, water and sodium polyacryl dimethyl taurate as a phase B, mixing and heating to 75 ℃ in proportion, and stirring for 15 minutes to make the material body uniform; adding the phase A into the phase B, and homogenizing for 20 minutes until the emulsion is uniform; stirring and cooling to 42 deg.C, adding the above Phaeodactylum algae and Tara fermented compound, homogenizing for 20 min to emulsify uniformly; adding plum blossom water in proportion, and stirring for 30 minutes to obtain the cream.
The formula of the face cream applied to the preparation method of the face cream is shown in the following table 4:
table 4 formula table (by weight percentage)
| Name of raw materials | Ratio/%) |
| Water (W) | 84.5 |
| Cocoa seed butter | 2 |
| Petrolatum | 1 |
| Glycerol stearate | 2 |
| PEG-100 stearate | 1 |
| Xanthan gum | 0.5 |
| Ascorbyl palmitate | 0.5 |
| 1, 2-pentanediol | 2 |
| Arginine | 1 |
| Sodium polyacryloyl dimethyl taurate | 0.5 |
| Phaeodactylum algae and tara fermentation compound | 2 |
| Plum blossom water | 3 |
The following introduces several components and beneficial effects related in the technical scheme of the invention:
phaeodactylum tricornutum extract: the product is rich in polyunsaturated fatty acid, and can activate 20S proteasome, accelerate degradation of carbonylation protein, and improve skin glossiness.
Tara pod extract: the gallic acid in the extract has strong effects of resisting oxidation, mutation and free radicals, can reduce the content of ROS, can improve endothelium dependence relaxation, shrink pores, and make skin fine and smooth.
Phaeodactylum algae and tara fermentation complex: can effectively reduce the protein carbonylation level in the skin, repair the skin and improve the skin glossiness in two modes of reducing the ROS content and activating the 20S proteasome under the double synergistic action in the molecule.
The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.
Claims (9)
1. A preparation method of a skin care product containing Phaeodactylum algae and tara fermentation compound is characterized by comprising the following steps: the method comprises the following steps:
s1, preparing Phaeodactylum algae and tara fermentation liquor, adding a tara pod extract into a glycerol aqueous solution, inoculating lactic acid bacteria, performing fermentation conversion reaction at the temperature of 20-40 ℃, adding a Phaeodactylum tricornutum extract, inoculating the lactic acid bacteria, and performing fermentation selective esterification reaction at the temperature of 35-50 ℃ to obtain the fermentation liquor;
s2, preparing a Phaeodactylum algae and tara fermentation compound, filtering or centrifuging the fermentation liquor, homogenizing and sterilizing the filtrate or the centrifugal supernatant to obtain the Phaeodactylum algae and tara fermentation compound;
s3, preparing a skin care product, namely selecting a part of skin care product raw materials as a phase A to be heated and uniformly mixed, selecting another part of skin care product raw materials as a phase B to be heated and uniformly mixed, then adding the phase B into the phase A or adding the phase A into the phase B to be subjected to homogeneous emulsification treatment, adding the phaeodactylum algae and tara fermentation compound to be subjected to homogeneous emulsification treatment after cooling, and finally adding hydrolat and stirring to obtain the skin care product;
the skin care product is essence or cream, the skin care product raw materials comprise a plurality of water, a humectant, a thickener, an emollient, an emulsifier and an antioxidant, and the lactic acid bacteria comprise one or more of lactobacillus casei, lactobacillus crispatus, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus formates, lactobacillus helveticus, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus salivarius.
2. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the concentration of the lactic acid bacteria participating in the reaction is 1X 109～9×1011CFU/mL, the inoculation amount of the lactobacillus is the volume of the solution, and the reaction time of the lactobacillus is 1-5 days.
3. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the tara pod extract is dissolved in 30-50% of glycerol aqueous solution by mass, and the mass ratio of the tara pod extract to the glycerol aqueous solution is 1: 19-99.
4. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the weight ratio of the phaeodactylum tricornutum extract to the tara pod extract is 1.5-5.
5. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the emollient comprises polydimethylsiloxane, hydrogenated olive oil ethylhexyl oleate, hydrogenated olive oil unsaponifiables, isononyl isononanoate, cyclopentadimethylsiloxane, cyclohexasiloxane, PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, caprylic/capric triglyceride, cocoa butter and petrolatum, the moisturizer comprises one or more of polyethylene glycol-32, glyceryl polyether-26, propylene glycol, allantoin, 1, 2-hexanediol, octylhydroximic acid, 1, 2-pentanediol, arginine, the thickener comprises one or more of sodium polyacryl dimethyl taurate, xanthan gum, stearic acid, the emulsifier comprises tris (laureth-4) phosphate, glyceryl stearate, PEG-100 stearate, glyceryl monostearate, PEG/or a mixture of any one or more of any of the foregoing compounds, and any of, One or more of behenyl alcohol and cetearyl ester.
6. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the antioxidant comprises one or more of tocopherol acetate, ascorbyl stearate and ascorbyl palmitate.
7. The method of claim 1, wherein the skin care product comprises a combination of Phaeodactylum algae and Tara fermentation broth, wherein the step of: the pure dew comprises one or more of bitter orange flower water, rose flower water, Egyptian blue water lily flower water, white flower chamomile flower water, hamamelis virginiana flower water, golden yellow chamomile flower water and plum flower water.
8. The method of preparing a skin care product comprising a combination of phaeodactylum algae and tara ferment according to any one of claims 1 to 7, wherein: the formula of the essence comprises the following components in parts by weight: 53-86 parts of water, 5-10 parts of a humectant, 1-3 parts of a thickener, 3-10 parts of an emollient, 1-7 parts of an emulsifier, 2-10 parts of a Phaeodactylum/tara fermentation compound and 2-7 parts of hydrolat.
9. The method of preparing a skin care product comprising a combination of phaeodactylum algae and tara ferment according to any one of claims 1 to 7, wherein: the formula of the face cream comprises the following components in parts by weight: 48-81.5 parts of water, 3-7 parts of a humectant, 1-3 parts of a thickener, 6-13 parts of an emollient, 3-10 parts of an emulsifier, 0.5-2 parts of an antioxidant, 2-10 parts of a phaeodactylum/tara fermentation compound and 3-7 parts of hydrolat.
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