CN111096941A

CN111096941A - Whitening composition containing symbiotic bacteria combined fermentation product, whitening essence and preparation method of whitening essence

Info

Publication number: CN111096941A
Application number: CN202010121369.3A
Authority: CN
Inventors: 龚德明; 范玉菡; 温伟红; 莫霖霭
Original assignee: Guangzhou Liujin Scientific Research Co Ltd
Current assignee: Guangzhou Biotechnology Co.,Ltd.
Priority date: 2020-02-26
Filing date: 2020-02-26
Publication date: 2020-05-05
Anticipated expiration: 2040-02-26
Also published as: CN111096941B

Abstract

The invention provides a whitening composition containing symbiotic bacteria combined leavening, whitening essence and a preparation method thereof. The whitening composition comprises the following components in parts by weight: 0.1-10 parts of tea extract, 0.01-5 parts of wolfberry fruit extract, 0.01-5 parts of butterflybush flower extract, 0.1-10 parts of water-caltrop bulb extract, 0.2-1 part of decarboxycarnosine HCl, 0.1-0.8 part of arbutin, 0.01-1 part of tranexamic acid, 2-5 parts of nicotinamide and 10-20 parts of symbiotic bacteria combined leavening. The whitening composition is scientifically compounded with various whitening active substances, has excellent antioxidant, anti-photoaging and anti-saccharification effects, can inhibit tyrosinase activity, promotes pigment metabolism, comprehensively improves the skin pigmentation problem, and enables the skin to be bright and flawless.

Description

Whitening composition containing symbiotic bacteria combined fermentation product, whitening essence and preparation method of whitening essence

Technical Field

The invention belongs to the field of cosmetic preparation, relates to whitening essence and a preparation method thereof, and particularly relates to a whitening composition containing symbiotic bacteria combined leavening, whitening essence and a preparation method thereof.

Background

The basal layer cells of human epidermis contain 4-5% of pigment cells, and the color of human skin is not dependent on the number of melanocytes, but on the number, size, distribution and melanosome degree.

Under the irradiation of ultraviolet rays, the keratinocytes release endothelin, and after the endothelin is combined with receptors on the cell membrane of melanin, the endothelin can stimulate the proliferation of the melanin cells, activate the activity of tyrosinase, and accelerate the growth of dendritic protrusions of the melanin cells, so that the synthesis of melanin and the migration of melanosomes to the keratinocytes are accelerated.

Because the reason for the formation of melanin is manifold, the reason for the formation of melanin must be comprehensively considered to make the product have a remarkable whitening effect. However, the multiple pathways act on the production and transfer processes of melanin, and the safety and stability of the product must be considered. Therefore, the whitening active substance with multiple functions, stability, safety and light color is selected to play the role of synergistic whitening, and the whitening composition has important significance.

CN109745244A discloses a whitening essence, which comprises: a nonionic emulsifier, an oil phase component, a polyol, vitamin C and derivatives thereof, inorganic salt and water; wherein the nonionic emulsifier comprises octyl dodecanol, octyl dodecanol xyloside and PEG-30 dipolyhydroxystearate; the oil phase component comprises hydrocarbon oil, synthetic fatty acid ester, animal and vegetable oil, silicone oil and mixture thereof. The whitening essence has high stability, but has single whitening component, single whitening way and single whitening channel, so that the whitening effect is slight, and the ideal whitening state cannot be achieved.

CN107890436A discloses a whitening antioxidant cosmetic, which comprises the following components in percentage by mass: 21-29% of liquid paraffin, 27-33% of olive oil, 0.5-2% of microcrystalline paraffin, 1-3% of vaseline, 1-5% of sorbitan monooleate, 1.5-3.5% of glycerol, 2.5-10% of whitening antioxidant, 3-4% of dimethyl sulfoxide, 3-10% of essence and the balance of deionized water. The invention has good antioxidant function, but easily causes skin sensitivity, has poor anti-inflammatory effect and undesirable whitening effect.

At present, the whitening essence generally has the problems of unobvious whitening effect, sensitive skin due to whitening components, damage to skin barriers, reduction of skin immunity and the like, cannot achieve the whitening effect, and can cause the skin to be gradually fragile and frequent.

Therefore, the development of a whitening essence which is mild and non-irritant and can achieve whitening effects in multiple aspects of oxidation resistance, photoaging resistance, saccharification resistance, tyrosinase activity inhibition, melanin synthesis reduction and the like is a problem to be solved in the field.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a whitening composition containing symbiotic bacteria combined with a fermented product, whitening essence and a preparation method thereof. The whitening composition can improve the glossiness, uniformity, permeability, whiteness and spot deposition of the skin from multiple aspects of oxidation resistance, photoaging resistance, saccharification resistance and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a whitening composition comprising a symbiotic bacteria combination ferment, the whitening composition comprising, in parts by weight:

0.1-10 parts of tea extract, 0.01-5 parts of wolfberry fruit extract, 0.01-5 parts of butterflybush flower extract, 0.1-10 parts of water-caltrop bulb extract, 0.2-1 part of decarboxycarnosine HCl, 0.1-0.8 part of arbutin, 0.01-1 part of tranexamic acid, 2-5 parts of nicotinamide and 10-20 parts of symbiotic bacteria combined leavening.

The whitening composition provided by the invention is scientifically compounded with various whitening active substances, oxidation resistance is realized through a tea extract and a wolfberry fruit extract, an anti-photoaging effect is realized through a butterflybush flower extract and a narcissus bulb extract, anti-saccharification is realized through decarboxylation of carnosine HCI, tyrosinase activity is inhibited through arbutin and tranexamic acid, pigment metabolism is promoted through nicotinamide, a symbiotic bacteria combined ferment is added, skin immunity can be improved, a synergistic effect exists between the symbiotic bacteria combined ferment and other whitening compositions, the whitening effect of the whitening composition can be further improved through the symbiotic bacteria combined ferment, skin glossiness, uniformity, permeability, whiteness and spot deposition can be comprehensively improved, and skin is bright and flawless.

In the present invention, the weight part of the tea (CAMELLIA SINENSIS) leaf extract is 0.1 to 10 parts, and may be, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 4 parts, 5 parts, 6 parts, 8 parts, 10 parts, or the like.

The tea extract has multiple functional components such as tea polyphenol, catechin and the like, has an inhibiting effect on caspase, can remove free radicals of a human body, and has excellent antioxidant capacity.

The weight portion of the medlar (Lycium CHINENSE) fruit extract is 0.01-5 parts, such as 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts or 5 parts.

The wolfberry fruit extract has strong antioxidant capacity, the absorption capacity of oxidizing free radicals of the wolfberry fruit extract is 25000 (compared with that of blueberry), the wolfberry fruit extract is rich in calcium, iron, zinc, selenium and vitamins, and meanwhile, the wolfberry fruit extract also has the effects of resisting genetic damage, maintaining normal development of cells, improving the repair capacity after DNA damage and enabling an organism to be younger.

The flos Buddlejae (Buddleja OFFICINALIS) extract is 0.01-5 parts, such as 0.01 parts, 0.05 parts, 0.1 parts, 0.5 parts, 1 part, 1.5 parts, 2 parts, 3 parts, 4 parts

Or 5 parts and the like.

The flos Buddlejae extract is derived from leaf of flos Buddlejae, contains multiple components such as glycyrrhizic acid, has effects of clearing heat and promoting diuresis, can be used in daily chemical products, and can effectively condition skin and improve skin health state.

The weight portion of the NARCISSUS bulb (NARCISSUS TAZETTA) extract is 0.1-10 parts, and can be, for example, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 4 parts, 5 parts, 6 parts, 8 parts or 10 parts, etc.

The stored nutrition of the scale leaves on the narcissus bulb is richer than that of the seeds, so that the narcissus bulb extract contains various plant proteins, alkaloids, glycosides and the like, and also contains tannin components, thereby having good skin astringing effect and simultaneously having the effects of moistening and conditioning the sebum secretion of the skin.

The weight portion of the decarboxylated carnosine HCl is 0.2 to 1 portion, and can be 0.2 portion, 0.3 portion, 0.4 portion, 0.5 portion, 0.6 portion, 0.7 portion, 0.8 portion, 0.9 portion or 1 portion, and the like.

The decarboxylated carnosine HCl acts on an oxidation path, prevents glycation in the past, and can be used for combining advanced glycation end products AGEs with induction factors instead of important collagen and elastic fibers so as to resist the glycation of skin.

The arbutin is 0.1-0.8 part by weight, such as 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part or 0.8 part.

The arbutin extracted from the leaves of Arctostaphylos uva-ursi can inhibit the activity of tyrosinase in vivo and prevent the generation of melanin, thereby reducing skin pigmentation, removing color spots and freckles, and also has antibacterial and anti-inflammatory effects.

The tranexamic acid is 0.01 to 1 part by weight, and may be, for example, 0.01 part, 0.05 part, 0.1 part, 0.2 part, 0.4 part, 0.5 part, 0.8 part, 0.9 part, or 1 part.

The whitening mechanism of tranexamic acid is to inhibit the activities of tyrosinase enzyme and melanocyte simultaneously and rapidly, and prevent melanin from aggregating, and eliminate the formation of melanin due to ultraviolet irradiation.

The weight portion of the nicotinamide is 2-5 portions, for example, 2 portions, 2.4 portions, 2.8 portions, 3 portions, 3.2 portions, 3.4 portions, 3.6 portions, 4 portions, 4.2 portions, 4.5 portions or 5 portions, etc.

Niacinamide is a derivative of vitamin B3, and the most important effect in anti-aging of skin is to reduce and prevent dullness, yellowing, and discoloration of skin color during early aging of skin. And meanwhile, the damaged stratum corneum lipid barrier can be repaired, and the skin resistance is improved. In the invention, 99% of high-purity nicotinamide is particularly selected, the nicotinic acid content is less than 150ppm, the irritation is reduced to the minimum, the skin tolerance is protected, and the skin is white and healthy.

The weight portion of the symbiotic bacteria combined fermentation product is 10-20 parts, and can be 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts or 20 parts and the like.

Preferably, the symbiotic bacteria of the symbiotic bacteria combined fermentation product comprise a combination of schizophyllum commune, thermus thermophilus, saccharomycetes and lactobacillus, and the fermentation raw materials of the symbiotic bacteria combined fermentation product comprise rice, Sichuan millet seeds, ginseng, kelp and raspberry.

Preferably, the mass ratio of schizophyllum commune, thermus thermophilus, yeast and lactobacillus in the symbiotic bacteria is (8-18): (4-8): (2-6): (1-3).

Wherein, the 8-18 can be 9, 10, 11, 12, 13, 14, 15, 16 or 17, etc.

The 4-8 can be 4.2, 4.5, 4.8, 5, 5.2, 5.5, 5.8, 6, 6.2, 6.5, 6.8, 7, 7.2, 7.5, 7.7, or 7.9, etc.

The 2-6 can be 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8, 5, 5.2, 5.5, 5.7, or 5.9, etc.

The 1-3 can be 1.2, 1.4, 1.6, 1.8, 2, 2.2, 2.4, 2.6, 2.8, 2.9, or the like.

Preferably, the mass ratio of the rice, the Sichuan millet seeds, the ginseng, the kelp and the raspberry in the fermentation raw materials is (40-60): (40-60):1: (20-30): 20-30).

In the above mass ratio, two of 40 to 60 may be 42, 44, 45, 46, 48, 50, 52, 54, 55, 56, 58, or the like, independently of each other; each of the two 20-30 independently can be 22, 24, 25, 26, 28, or 30, etc.

Preferably, the mass sum of the symbiotic bacteria is 20-30% of the mass sum of the fermentation raw materials, and may be, for example, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, or the like.

In the invention, the preparation method of the symbiotic bacteria combined fermentation product comprises the following steps:

(1) pulverizing rice, Sichuan millet seed, ginseng, kelp and raspberry, mixing with water to obtain a to-be-fermented solution, and sterilizing;

(2) inoculating yeast and lactobacillus to the sterilized liquid to be fermented obtained in the step (1) for primary fermentation, then adding schizophyllum commune for secondary fermentation, and finally adding thermophilic thermus bacteria for tertiary fermentation to obtain the symbiotic bacteria combined fermentation product.

In the preparation process of the symbiotic bacteria combined fermentation product, different living environments of different strains are considered, so that the fermentation process is performed step by step, firstly, yeast and lactobacillus are subjected to enriched fermentation, then schizophyllum commune is added for fermentation, the yeast and the lactobacillus have an induced activation effect on the schizophyllum commune, the generation of schizophyllum commune can be increased, the generated schizophyllum commune further has an accelerating effect on the yeast and the lactobacillus to accelerate the fermentation of the yeast and the lactobacillus to raw materials, and finally, thermophilic thermus bacteria are added, and the yeast also has an induced activation effect on the thermophilic thermus bacteria, so that the high-activity symbiotic bacteria combined fermentation product is obtained.

In the present invention, the method of culturing yeast, lactobacillus, thermus thermophilus, and schizophyllum to satisfy the inoculation requirements in terms of the number and activity is not particularly limited, and any conventional method in the prior art may be used.

In a preferred embodiment of the present invention, the whitening composition further comprises 0.1 to 0.5 parts (for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, or 0.5 part, etc.) potassium azelaidate and 0.5 to 2 parts (for example, 0.5 part, 0.8 part, 1 part, 1.5 parts, or 2 parts, etc.) hydrolyzed pearl.

Preferably, the whitening composition comprises 1-5 parts by weight of tea extract, 0.01-1 part by weight of boxthorn fruit extract, 0.01-1 part by weight of butterflybush flower extract, 1-5 parts by weight of water caltrop bulb extract, 0.2-1 part by weight of decarboxypeptide HCl, 0.1-0.5 part by weight of arbutin, 0.01-1 part by weight of tranexamic acid, 2-5 parts by weight of niacinamide, 0.1-0.5 part by weight of potassium azeloyldiglycinate and 0.5-2 parts by weight of hydrolyzed pearl.

In a second aspect, the present invention provides a whitening essence comprising the whitening composition of the first aspect.

As a preferred technical scheme of the present invention, the whitening essence comprises, by mass:

5-35% of the whitening composition of the first aspect, 5-27% of a humectant, 8-42% of an emollient, 2.5-8.5% of an emulsifier, 0.02-0.1% of a chelating agent, 0.01-1% of a thickener, 0.9-1.6% of an antioxidant, and the balance of rose water.

The whitening essence provided by the invention contains the whitening composition of the first aspect, can improve the skin glossiness from multiple aspects, has a good moisturizing effect, and can make the skin smooth and bright.

The whitening composition of the present invention may be 5 to 35% by mass, for example, 5%, 8%, 10%, 15%, 18%, 20%, 22%, 25%, 28%, 30%, 35% or the like.

In the present invention, the humectant may be 5 to 27% by mass, for example, 5%, 8%, 10%, 12%, 14%, 16%, 20%, 22%, 24%, 25%, 27%, or the like.

The emollient may be present in an amount of 8-42% by weight, for example 8%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, or 42% by weight.

The emulsifier in the present invention may be present in an amount of 2.5 to 8.5% by mass, for example, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, or 8.5%.

The chelating agent in the present invention may be 0.02 to 0.1% by mass, for example, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, or 0.1%.

The thickener in the present invention may be contained in an amount of 0.01 to 1% by mass, for example, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, or 0.1%.

In the present invention, the antioxidant may be contained in an amount of 0.9 to 1.6% by mass, for example, 0.9%, 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, or 1.6%.

In a preferred embodiment of the present invention, the humectant includes one or a combination of at least two of sodium hyaluronate, trehalose, saccharide isomers, 1, 2-hexanediol, butanediol, xylitol, anhydroxylitol, xylitol-based glucoside, panthenol, and glycerin.

Preferably, the emollient comprises any one or a combination of at least two of squalane, shea butter, canola oil, dioctyl carbonate, caprylic/capric triglyceride, dimethicone, or stearic acid.

Preferably, the emulsifier comprises any one of or a combination of at least two of sodium acrylate copolymer, lecithin, glyceryl stearate, PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, cetearyl alcohol or cetearyl glucoside.

Preferably, the chelating agent comprises disodium edetate.

Preferably, the thickening agent comprises hydrolyzed sclerotium rolfsii gum.

Preferably, the antioxidant comprises p-hydroxyacetophenone and/or tocopherol acetate.

As a preferable technical scheme of the invention, the whitening essence further comprises 0.9-10% of a skin conditioner by mass.

Preferably, the skin conditioner comprises any one or a combination of at least two of a truffle extract, ceramide, a sophora flavescens root extract, a glycyrrhiza inflata root extract, a scutellaria baicalensis root extract, retinol palmitate, or dipotassium glycyrrhizinate.

5-35% of the whitening composition of the first aspect, 0.01-0.1% sodium hyaluronate, 0.5-2% trehalose, 0.3-2% saccharide isomerate, 0.4-0.6% 1, 2-hexanediol, 2-10% butanediol, 0.01-0.2% xylitol, 0.01-0.3% anhydroxylitol, 0.1-0.5% xylitol-based glucoside, 0.3-1% panthenol, 2-10% glycerol, 1-5% squalane, 1-5% shea butter, 1-5% canola oil, 2-10% dioctyl carbonate, 2-10% caprylic/capric triglyceride, 1-5% polydimethylsiloxane, 0.5-2% stearic acid, 0.07-0.35% acrylic ester copolymer sodium, 0.03-0.15% lecithin, 0.25-1% glyceryl stearate, 0.25-1% PEG-100 stearate, 1-3% polyglycerol-3 methyl glucose distearate, 0.8-2.4% cetearyl alcohol, 0.2-0.6% cetearyl glucoside, 0.01-1% truffle extract, 0.5-6% ceramide, 0.01-0.5% Ku Shen root extract, 0.01-0.5% Licorice root extract, 0.01-0.5% Scutellaria root extract, 0.3-1% retinol palmitate, 0.1-0.5% dipotassium glycyrrhizinate, 0.02-0.1% chelating agent, 0.01-1% thickening agent, 0.4-0.6% p-hydroxyacetophenone, 0.5-1% tocopherol acetate, and the balance rose water.

In a third aspect, the present invention provides a method for preparing the whitening essence according to the second aspect, the method comprising the steps of:

(1) mixing the water phase components in the humectant, the emollient and the emulsifier with the rose water to obtain water phase mixed solution, and mixing the oil phase components in the humectant, the emollient and the emulsifier to obtain oil phase mixed solution;

(2) mixing and stirring the water phase mixed solution and the oil phase mixed solution obtained in the step (1) to obtain a mixed solution;

(3) and (3) mixing the mixed solution obtained in the step (2), the whitening composition, the chelating agent, the thickening agent and the antioxidant according to the formula amount, so as to obtain the whitening essence.

As a preferred embodiment of the present invention, the temperature of the mixture of the aqueous phase component and the rose water in step (1) is 80 to 85 ℃, and may be, for example, 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, or 85 ℃; the time is 5-10min, such as 5min, 5.5min, 6min, 6.5min, 7min, 7.5min, 8min, 8.5min, 9min, 9.5min or 10 min.

Preferably, the temperature for mixing the oil phase components in the step (1) is 78-80 ℃, for example 78 ℃, 78.5 ℃, 79 ℃, 79.5 ℃ or 80 ℃ and the like; the time is 5-10min, such as 5min, 5.5min, 6min, 6.5min, 7min, 7.5min, 8min, 8.5min, 9min, 9.5min or 10 min.

Preferably, the mixing and stirring temperature in the step (2) is 80-82 ℃, for example, 80 ℃, 80.5 ℃, 81 ℃, 81.5 ℃ or 82 ℃ and the like; the time is 10-15min, such as 10min, 11min, 12min, 13min, 14min or 15 min.

Preferably, the temperature of the mixing in the step (3) is 40-45 ℃, for example, 40 ℃, 41 ℃, 42 ℃, 43 ℃, 44 ℃ or 45 ℃ and the like; the time is 10-20min, such as 10min, 11min, 12min, 13min, 14min, 15min, 16min, 17min, 18min, 19min or 20 min.

As a preferred technical scheme of the invention, the preparation method comprises the following steps:

(1) adding water phase components of humectant, emollient and emulsifier and flos Rosae Rugosae water into water phase pot, stirring and mixing at 80-85 deg.C for 5-10min to obtain water phase mixed solution; adding oil phase components of humectant, emollient and emulsifier into oil phase pan, stirring and mixing at 78-80 deg.C for 5-10min to obtain oil phase mixed solution;

(2) pumping the water phase mixed liquid and the oil phase mixed liquid obtained in the step (1) into an emulsifying pot, and mixing and stirring at 80-82 ℃ for 10-15min to obtain a mixed liquid;

(3) and (3) stirring and mixing the mixed solution obtained in the step (2), the whitening composition, the chelating agent, the thickening agent and the antioxidant in formula amounts according to the first aspect at 40-45 ℃ for 10-20min, cooling to below 37 ℃, and discharging to obtain the whitening essence.

The recitation of numerical ranges herein includes not only the above-recited numerical values, but also any numerical values between non-recited numerical ranges, and is not intended to be exhaustive or to limit the invention to the precise numerical values encompassed within the range for brevity and clarity.

Compared with the prior art, the invention has the beneficial effects that:

(1) the whitening composition provided by the invention is scientifically compounded with various whitening active substances, and through the mutual cooperation of the tea extract, the wolfberry fruit extract, the butterflybush flower extract, the narcissus bulb extract, the decarboxycarnosine HCI, the arbutin, the tranexamic acid, the nicotinamide and the symbiotic bacteria combined leavening, the whitening active substances are synergistic, the pigment metabolism is promoted, the antioxidant, anti-photoaging and anti-saccharification effects are improved, and meanwhile, the tyrosinase activity can be inhibited, so that the skin is bright and flawless; meanwhile, the added symbiotic bacteria combined leavening can improve the skin immunity and further improve the whitening effect of the whitening composition, and meanwhile, the whitening essence does not contain risk components such as essence, pigment, hormone, lead, mercury and the like, so that the skin immunity, tolerance and repairing capacity are enhanced, the skin is whitened more safely and is more tenacious;

(2) the whitening essence provided by the invention has the advantages that the DPPH free radical clearance rate is 70.1-76.9%, the ROS epidermal keratinocyte accumulation amount is 8.7-13.6%, the glycation inhibition rate is 54.6-69.9%, the relative tyrosinase activity is 18.4-23.7% and the relative melanin content is 10.2-13.4% after the whitening essence provided by the invention is used, meanwhile, the facial melanin content of a subject can be obviously reduced, and the melanin content reduction value is 43.3-53.9%.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

In the following examples, the symbiotic combination fermentate may be prepared by the following method:

(1) pulverizing 33.11g rice, 33.11g Sichuan millet seed, 0.66g Ginseng radix, 16.56g Macrocystis and 16.56g Rubi fructus, mixing with 180g water, filtering with 80 mesh filter cloth to obtain fermentation solution, and heating at 100 deg.C for 20min for sterilization;

(2) adjusting pH of the sterilized solution to be fermented to 4.5 with arginine, placing in a seed tank, inoculating yeast 4g and lactobacillus 2g, and culturing at 5m³Aeration amount of/h/50L, stirring and rotating at 150rpm, and culturing at 30 ℃ for 22 h;

(3) then adding arginine to adjust pH to 6.5, adding 12g schizophyllum commune at 5m³Aeration amount of/h/50L, stirring and rotating at 150rpm, and culturing at 30 ℃ for 22 h;

(4) finally, arginine was used to adjust the pH to 8.0, 6g of Thermus thermophilus was added at 5m³Stirring and rotating at 150rpm for ventilation amount of 50L/h, culturing at 35 ℃ for 22h, and then raising the temperature to 58 ℃ for culturing for 18h to obtain a primary product;

(5) filtering the primary product, taking clear liquid, adding active carbon into the clear liquid, stirring for 10min, performing adsorption and purification treatment, taking clear liquid again by using a filtering mode, and sterilizing to obtain the symbiotic bacteria combined fermentation product.

Wherein the rice is northeast rice, fructus oryzae is from Guizhou, Ginseng radix is Changbai mountain Ginseng radix, Macrocystis is from Shandong Changdao county, and Rubi fructus is from Anhui. The yeast is selected from cerevisiae Fermentum, and the lactobacillus is selected from Lactobacillus thermophilus.

Example 1

Provided is a whitening composition, which comprises the following components in parts by weight:

2 parts of tea extract, 0.5 part of boxthorn fruit extract, 0.5 part of buddleja officinalis extract, 3 parts of clematis chinensis bulb extract, 0.5 part of carnosine decarboxylation HCl, 0.3 part of arbutin, 0.5 part of tranexamic acid, 3 parts of nicotinamide, 0.25 part of potassium azelaiyl diglycinate, 1.25 parts of hydrolyzed pearl and 15 parts of symbiotic bacteria combined ferment.

Example 2

5 parts of tea extract, 1 part of wolfberry fruit extract, 1 part of butterflybush flower extract, 5 parts of water-caltrop bulb extract, 1 part of carnosine decarboxylation HCl, 0.1 part of arbutin, 0.1 part of tranexamic acid, 2 parts of nicotinamide, 0.1 part of potassium azeloyl diglycinate, 0.5 part of hydrolyzed pearl and 20 parts of symbiotic bacteria combined leavening.

Example 3

1 part of tea extract, 0.01 part of boxthorn fruit extract, 0.01 part of buddleja officinalis extract, 1 part of clematis chinensis bulb extract, 0.2 part of carnosine decarboxylation HCl, 0.5 part of arbutin, 1 part of tranexamic acid, 5 parts of nicotinamide, 0.5 part of potassium azelaiyl diglycinate, 2 parts of hydrolyzed pearl and 15 parts of symbiotic bacteria combined ferment.

Example 4

0.1 part of tea extract, 0.01 part of boxthorn fruit extract, 0.01 part of buddleja officinalis extract, 0.1 part of water caltrop bulb extract, 0.2 part of decarboxypeptide HCl, 0.1 part of arbutin, 0.01 part of tranexamic acid, 2 parts of nicotinamide, 0.1 part of potassium azelaioyl diglycinate, 2 parts of hydrolyzed pearl and 16 parts of symbiotic bacteria combined ferment.

Example 5

10 parts of tea extract, 5 parts of wolfberry fruit extract, 5 parts of butterflybush flower extract, 10 parts of water-caltrop bulb extract, 1 part of carnosine decarboxylation HCl, 0.8 part of arbutin, 1 part of tranexamic acid, 5 parts of nicotinamide and 12 parts of symbiotic bacteria combined leavening.

Example 6

A whitening composition is provided, which is different from example 1 in that the weight part increase of the symbiotic bacteria combination fermented product is 20 parts.

Example 7

A whitening composition is provided, which is different from example 1 in that the weight part of the symbiotic bacteria combination fermented product is reduced to 10 parts.

Comparative examples 1 to 9

Is a single whitening active substance and comprises the following components:

tea leaf extract (comparative example 1), buddleja officinalis extract (comparative example 2), wolfberry fruit extract (comparative example 3), narcissus bulb extract (comparative example 4), carnosine decarboxylation HCl (comparative example 5), arbutin (comparative example 6), tranexamic acid (comparative example 7), niacinamide (comparative example 8), symbiotic bacteria combination fermentation (comparative example 9).

Comparative examples 10 to 18

The whitening composition obtained by compounding different whitening active substances is shown in the following table 1 according to the formula of each proportion by weight:

TABLE 1

Application examples 1 to 7

The whitening essence comprises the following components in parts by mass:

the whitening compositions applied in the embodiments 1 to 7 are the whitening compositions provided in the embodiments 1 to 7. The preparation method comprises the following steps:

(1) adding water phase components of humectant, emollient and emulsifier and flos Rosae Rugosae water into water phase pot, stirring and mixing at 80 deg.C for 5min to obtain water phase mixed solution; adding oil phase components of humectant, emollient and emulsifier into oil phase pan, stirring and mixing at 78 deg.C for 5min to obtain oil phase mixed solution;

(2) pumping the water phase mixed liquid and the oil phase mixed liquid obtained in the step (1) into an emulsifying pot, and mixing and stirring at 80 ℃ for 10min to obtain a mixed liquid;

(3) and (3) stirring and mixing the mixed solution obtained in the step (2), the whitening composition, the chelating agent, the thickening agent and the antioxidant according to the formula ratio at 40 ℃ for 20min, cooling to below 37 ℃, and discharging to obtain the whitening essence.

Application example 8

The whitening essence is provided, and compared with application comparative example 1, the only difference is that the content of the whitening composition in the whitening essence is increased to 35%, and the rest components and content are the same as those in application example 1.

Application example 9

The whitening essence is provided, and compared with application comparative example 1, the only difference is that the content of the whitening composition in the whitening essence is reduced to 5%, and the rest components and content are the same as those in application example 1.

Application comparative examples 1 to 18

Compared with the application comparative example 1, the whitening composition is respectively replaced by the whitening active substances or the whitening composition provided by the comparative examples 1-18, and the rest components and the content are the same as those in the application example 1.

Comparative application example 19

Compared with the application comparative example 1, the whitening composition is not contained, and the rest components and the content are the same as the application example 1.

Performance test 1

The safety performance test is carried out on the whitening essence provided by the application examples 1-9 and the application comparative examples 1-19, and the method comprises the following steps:

(1) haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Selected samples were diluted to different concentrations with PBS solution, 5 per sample setA concentration gradient. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing its absorbance at 560nm with spectrophotometer (A)₅₆₀) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated₅₀)。

(2) Protein denaturation experiments:

diluting the sample to 10g/L with PBS solution, collecting 10mL dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing absorbance A at 540nm and 575nm with spectrophotometer₅₄₀And A₅₇₅Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 2 below:

TABLE 2

L/D	Grading
		>100	Has no irritation
10<L/D≤100	Micro-stimulation property
		1<L/D≤10	Mild irritation
0.1<L/D≤1	Moderate irritation

The results of the above-described hemolysis test and protein denaturation test are shown in Table 3 below:

TABLE 3

According to safety performance tests, the L/D values of the whitening essence provided by the invention are all larger than 100, which indicates that the whitening essence is mild and non-irritant, and the increase of the content of the symbiotic bacteria combined fermentation product can reduce the irritation of the whitening essence and ensure that the obtained product is safer and more reliable as compared with the application examples 1, 6, 7 and 18.

Performance test 2

The whitening essence provided by the application examples 1-9 and the application comparative examples 1-19 is subjected to oxidation resistance, saccharification resistance and photoaging resistance tests, and the specific method comprises the following steps:

(1) oxidation resistance test

Evaluation of DPPH radical scavenging ability: the ability to scavenge DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) free radicals is to some extent the antioxidant ability of the reaction mass. The greater the free radical clearance rate, the stronger the antioxidant capacity and the stronger the anti-aging capacity. Therefore, the anti-aging effect of the traditional Chinese medicine composition can be judged by researching the capability of the traditional Chinese medicine composition for eliminating DPPH free radicals.

The samples were diluted with DMSO to 2% mass fraction of the sample to be tested and the following tests were performed: adding 2.0mL of DPPH test solution with mass concentration of 45.8mg/L and a certain amount of sample to be tested into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking up, reacting in a dark place for 30min, and measuring the absorbance at the wavelength of 517nm by using a 1cm cuvette, and recording as A₁₀(ii) a Adding 2mL of absolute ethyl alcohol and a sample to be detected with a corresponding volume into the test tube, supplementing the total volume to 3mL by the absolute ethyl alcohol, and recording the measured absorbance as A₁₁(ii) a Adding 2mL of DPPH test solution and 1mL of absolute ethanol into the test tube, and recording the measured absorbance as A₁₂Calculating the DPPH free radical clearance rate (Y') of the liquid to be detected;

the DPPH free radical clearance is calculated by the formula:

in the formula A₁₀Absorbance value of system after removing DPPH for sample to be measured, A₁₁Absorbance value of the System after eliminating DPPH for sample blank control, A₁₂The absorbance values of the reaction system before adding no drug are shown in Table 4.

(2) Light aging resistance test

The oxidative stress test of cuticle keratinocyte under blue light comprises the following specific test methods: oxidation of organotypic skin model using blue radiation, pretreated with a test sample anti-photoaging compositionIrradiating with blue light with intensity of 15J/cm². Since moderate and high doses of ROS (reactive oxygen species) induce apoptosis and even cause necrosis of cells through oxidative stress of the cells, measuring the accumulation of ROS in epidermal keratinocytes reflects the photoaging resistance of the test samples, and the test results are shown in table 4.

(3) Anti-glycation assay

280 female volunteers aged 18-50 years are selected, the whitening essences provided in application examples 1-9 and application comparative examples 1-19 are respectively used on the face, the subjects are used twice a day for 30 days continuously, and no other product is used in the test period. The glycation inhibition results obtained by the test using the german MAP580 skin tester are shown in table 4 below:

TABLE 4

The whitening essence provided by the invention has obvious antioxidant, anti-photoaging and anti-saccharification effects, the DPPH free radical clearance rate of the whitening essence is 70.1-76.9%, the ROS epidermal keratinocyte accumulation amount is 8.7-13.6%, and the saccharification inhibition rate is 54.6-69.9%, which indicates that the whitening essence can effectively eliminate free radicals, inhibit the generation of ROS in human epidermal keratinocytes under the blue light condition, further protect skin cells from being damaged by blue light irradiation, and inhibit saccharification reaction at the same time. Therefore, the whitening essence provided by the invention can realize whitening effect from multiple aspects and make the skin healthy and fair.

Performance test 3

The whitening effect test is carried out on the whitening essence provided by the application examples 1-9 and the application comparative examples 1-19, and the method comprises the following steps:

(1) tyrosinase activity inhibition assay

Mouse melanoma B16 cells were harvested from log phase growth and plated onto 6-well cell culture plates for overnight culture. The test samples with a final volume fraction of 1% were added to each of the wells, and the untreated group was used as a cell control group, and each of the wells was replicated in 2 wells. After 48h of culture, the cells were washed 1 time with PBS, 100. mu.L of lysate was added to each well, the cells were scraped off and collected, and the supernatant was centrifuged. 50 μ L of cell supernatant was applied to a 96-well plate, 50 μ L of 1% L-dopa solution was added, incubation was performed at 37 ℃ for 1h, and absorbance was read at 475nm using M3 plate reader.

Relative tyrosinase activity (%) - (assay well absorbance-blank absorbance)/(cell control absorbance-blank absorbance) × 100%.

(2) Experiment for inhibiting melanin synthesis

Mouse melanoma B16 cells in logarithmic growth phase were inoculated into T25 cell culture flasks and cultured overnight. The test samples with a final volume fraction of 1% were added to each of the untreated groups as a cell control group. After 48h of incubation, the cells were washed 1 time with PBS, 1mL of 1mol/L NaOH solution was added, the collected cells were scraped, placed in a water bath at 80 ℃ for 30min, the supernatant was added to a 96-well plate, and the absorbance was read at 475nm using an M3 plate reader.

Relative melanin content (%) ═ 100% (assay well absorbance value-blank absorbance value)/(cell control absorbance value-blank absorbance value). The results of the tyrosinase activity and melanin synthesis inhibition experiments are shown in table 5, wherein the tyrosinase activity of the control group is 100%, and the melanin content of the control group is 100%.

(3) Human body evaluation of whitening effect

280 female volunteers aged 18-50 years are selected, and the whitening essence provided by application examples 1-9 and application comparative examples 1-19 is respectively used on the face for 1 time in the morning and evening every day for 4 weeks.

The skin melanin content changes before and after application of the mask composition were evaluated by a skin melanin tester (Hexameter MX 18). The measurement range of the used instrument is 0-999, and the higher the measurement value, the higher the melanin content in the skin is. The amount of melanin reduction in the back and front skin of the subject before application of the mask composition prepared according to the present invention is shown in table 5.

TABLE 5

The results in the table show that the relative tyrosinase activity is 18.4-23.7% after the whitening essence provided by the invention is used, the inhibition effect on tyrosinase is good, the synthesis of melanin in cells can be obviously inhibited, the relative melanin content is 10.2-13.4%, and the results of human body evaluation show that the whitening essence provided by the invention can obviously reduce the melanin content of a subject after being used for 4 weeks, and the reduction value of the melanin content on the face of the subject is 43.3-53.9%, which indicates that the whitening effect of the whitening essence is good; and as can be seen from comparison of application examples 1, 6-9 and application comparative example 18, the addition of the symbiotic bacteria combined fermentation product can significantly improve the whitening effect of the whitening essence, and the effect is significantly increased with the increase of the addition amount, wherein the reduction amount of melanin of the subject in application example 8 is up to 53.9%.

In conclusion, the whitening essence provided by the invention is mild and non-irritant, has obvious antioxidant, anti-photoaging and anti-saccharification effects, can inhibit the activity of tyrosinase, reduces the synthesis amount of melanin in cells, and has more excellent effect compared with a whitening composition (application comparative examples 10-18) prepared by compounding a single whitening active substance (application comparative examples 1-9) or lacking a certain whitening active substance, and meanwhile, when the symbiotic bacteria combined fermentation product and other whitening active substances are used at the same time, the safety and whitening effect of the whitening essence can be obviously improved.

The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.

Claims

1. The whitening composition containing symbiotic bacteria combined leavening is characterized by comprising the following components in parts by weight:

2. The whitening composition according to claim 1, wherein the symbiotic bacteria of the symbiotic bacteria combined fermentation product comprise a combination of schizophyllum commune, thermus thermophilus, yeast and lactobacillus, and the fermentation raw materials of the symbiotic bacteria combined fermentation product comprise rice, Sichuan millet seeds, ginseng, kelp and raspberry;

preferably, the mass ratio of schizophyllum commune, thermus thermophilus, yeast and lactobacillus in the symbiotic bacteria is (8-18): (4-8): (2-6): (1-3);

preferably, the mass ratio of the rice, the Sichuan millet seeds, the ginseng, the kelp and the raspberry in the fermentation raw materials is (40-60): (40-60):1: (20-30): 20-30);

preferably, the mass sum of the symbiotic bacteria is 20-30% of the mass sum of the fermentation raw materials.

3. The whitening composition of claim 1 or 2, further comprising 0.1 to 0.5 parts by weight of potassium azeloyl diglycinate and 0.5 to 2 parts by weight of hydrolyzed pearls;

preferably, the whitening composition comprises 1-5 parts of tea extract, 0.01-1 part of wolfberry fruit extract, 0.01-1 part of butterflybush flower extract, 1-5 parts of clematis chinensis bulb extract, 0.2-1 part of carnosine decarboxylation HCl, 0.1-0.5 part of arbutin, 0.01-1 part of tranexamic acid, 2-5 parts of nicotinamide, 0.1-0.5 part of potassium azeloyl diglycinate, 0.5-2 parts of hydrolyzed pearl and 10-20 parts of symbiotic bacteria combined leavening in parts by weight.

4. Whitening essence characterized in that it comprises the whitening composition according to any one of claims 1 to 3.

5. The whitening essence according to claim 4, comprising by mass:

5-35% of the whitening composition according to any one of claims 1-3, 5-27% of a humectant, 8-42% of an emollient, 2.5-8.5% of an emulsifier, 0.02-0.1% of a chelating agent, 0.01-1% of a thickener, and 0.9-1.6% of an antioxidant, the balance being rose water.

6. The whitening essence according to claim 5, wherein the humectant comprises any one or a combination of at least two of sodium hyaluronate, trehalose, saccharide isomers, 1, 2-hexanediol, butanediol, xylitol, anhydroxylitol, xylitol-based glucoside, panthenol or glycerin;

preferably, the emollient comprises any one or a combination of at least two of squalane, shea butter, canola oil, dioctyl carbonate, caprylic/capric triglyceride, dimethicone, or stearic acid;

preferably, the emulsifier comprises any one of or a combination of at least two of acrylate copolymer sodium, lecithin, glyceryl stearate, PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, cetearyl alcohol or cetearyl glucoside;

preferably, the chelating agent comprises disodium ethylenediaminetetraacetate;

preferably, the thickening agent comprises hydrolyzed sclerotium rolfsii gum;

preferably, the antioxidant comprises p-hydroxyacetophenone and/or tocopherol acetate;

preferably, the whitening essence further comprises 0.9-10% of a skin conditioning agent in percentage by mass;

7. The whitening essence according to claim 5 or 6, characterized by comprising in mass percent:

5-35% of the whitening composition of any one of claims 1-3, 0.01-0.1% sodium hyaluronate, 0.5-2% trehalose, 0.3-2% saccharide isomerate, 0.4-0.6% 1, 2-hexanediol, 2-10% butylene glycol, 0.01-0.2% xylitol, 0.01-0.3% anhydroxylitol, 0.1-0.5% xylitol-based glucoside, 0.3-1% panthenol, 2-10% glycerol, 1-5% squalane, 1-5% shea butter, 1-5% canola oil, 2-10% dioctyl carbonate, 2-10% caprylic/capric triglyceride, 1-5% polydimethylsiloxane, 0.5-2% stearic acid, 0.07-0.35% acrylic acid ester copolymer sodium, 0.03-0.15% lecithin, lecithin, 0.25-1% of glyceryl stearate, 0.25-1% of PEG-100 stearate, 1-3% of polyglycerol-3 methyl glucose distearate, 0.8-2.4% of cetearyl alcohol, 0.2-0.6% of cetearyl glucoside, 0.01-1% of truffle extract, 0.5-6% of ceramide, 0.01-0.5% of sophora flavescens ait root extract, 0.01-0.5% of glycyrrhiza inflate ait root extract, 0.01-0.5% of scutellaria baicalensis root extract, 0.3-1% of retinol palmitate, 0.1-0.5% of dipotassium glycyrrhizinate, 0.02-0.1% of chelating agent, 0.01-1% of thickening agent, 0.4-0.6% of p-hydroxyacetophenone, 0.5-1% of tocopherol acetate, and the balance of rose water.

8. A method for preparing the whitening essence according to any one of claims 5 to 7, comprising the steps of:

(3) mixing the mixed solution obtained in the step (2), the whitening composition according to any one of claims 1 to 3, a chelating agent, a thickener and an antioxidant in a formula amount to obtain the whitening essence.

9. The method according to claim 8, wherein the temperature of the aqueous phase component mixed with the rose water in step (1) is 80 to 85 ℃ for 5 to 10 min;

preferably, the temperature for mixing the oil phase components in the step (1) is 78-80 ℃ and the time is 5-10 min;

preferably, the mixing and stirring temperature in the step (2) is 80-82 ℃, and the time is 10-15 min;

preferably, the mixing in step (3) is carried out at 40-45 deg.C for 10-20 min.

10. The method of manufacturing according to claim 8 or 9, comprising the steps of:

(3) stirring and mixing the mixed solution obtained in the step (2), the whitening composition according to any one of claims 1 to 3, the chelating agent, the thickening agent and the antioxidant in formula amounts at 40-45 ℃ for 10-20min, cooling to below 37 ℃, and discharging to obtain the whitening essence.