CN113166803A - 一种基于芯片引物表面萃取的基因高通量合成方法 - Google Patents
一种基于芯片引物表面萃取的基因高通量合成方法 Download PDFInfo
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Abstract
提供一种基于芯片引物表面萃取的基因高通量合成方法，该方法将引物按簇分类后合成在芯片的固定区域并进行切割，随后使用取样机将不同簇的引物溶解并转移至96孔板中进行基因合成。
Description
PCT国内申请，说明书已公开。
Claims (30)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811313909 | 2018-11-06 | ||
| CN2018113139097 | 2018-11-06 | ||
| PCT/CN2019/115911 WO2020094040A1 (zh) | 2018-11-06 | 2019-11-06 | 一种基于芯片引物表面萃取的基因高通量合成方法 |
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| Publication Number | Publication Date |
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| CN113166803A true CN113166803A (zh) | 2021-07-23 |
| CN113166803B CN113166803B (zh) | 2024-04-30 |
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| CN201980072942.1A Active CN113166803B (zh) | 2018-11-06 | 2019-11-06 | 一种基于芯片引物表面萃取的基因高通量合成方法 |
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| Country | Link |
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| CN (1) | CN113166803B (zh) |
| TW (1) | TWI845557B (zh) |
| WO (1) | WO2020094040A1 (zh) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013096839A1 (en) | 2011-12-22 | 2013-06-27 | Somagenics, Inc. | Methods of constructing small rna libraries and their use for expression profiling of target rnas |
| EP3394292B1 (en) | 2015-12-21 | 2021-04-28 | RealSeq Biosciences, Inc. | Methods of library construction for polynucleotide sequencing |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870717A (zh) * | 2010-05-28 | 2010-10-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种寡核苷酸“直接缩合法”试剂及其用途 |
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| BRPI0721095B1 (pt) * | 2006-12-13 | 2015-09-29 | Luminex Corp | Sistemas e métodos para a análise multíplex de pcr em tempo real |
| TWI684644B (zh) * | 2007-08-13 | 2020-02-11 | 美商網路生物有限公司 | 利用電泳偵測核酸及生物分子之整合微流體系統、生物晶片及方法 |
| KR101287431B1 (ko) * | 2010-05-07 | 2013-07-19 | (주)진매트릭스 | 표적 유전자의 다양한 변이가 존재하는 유전자 영역을 증폭하기 위한 프라이머 조성물, 이를 이용한 표적 유전자 증폭 방법 및 이를 포함하는 ｐｃｒ 증폭 키트 그리고 이를 이용한 표적 유전자의 유전자형 분석방법 |
| CN101906476A (zh) * | 2010-07-30 | 2010-12-08 | 中国科学院苏州纳米技术与纳米仿生研究所 | 一种基于悬浮芯片的多重固相扩增检测方法 |
| CN106674283A (zh) * | 2016-12-15 | 2017-05-17 | 中国科学院北京基因组研究所 | 可完全去疤的可逆末端终止功能核苷酸及其应用 |
| CN108395462B (zh) * | 2017-12-13 | 2021-10-01 | 通用生物系统（安徽）有限公司 | 一种新型的y-str基因座检测用dna探针合成方法 |
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2019
- 2019-11-06 CN CN201980072942.1A patent/CN113166803B/zh active Active
- 2019-11-06 TW TW108140278A patent/TWI845557B/zh active
- 2019-11-06 WO PCT/CN2019/115911 patent/WO2020094040A1/zh active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870717A (zh) * | 2010-05-28 | 2010-10-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 一种寡核苷酸“直接缩合法”试剂及其用途 |
Non-Patent Citations (1)
| Title |
|---|
| EROSHENKO N等: "Gene Assembly from Chip-Synthesized Oligonucleotides" * |
Also Published As
| Publication number | Publication date |
|---|---|
| TW202045732A (zh) | 2020-12-16 |
| CN113166803B (zh) | 2024-04-30 |
| TWI845557B (zh) | 2024-06-21 |
| WO2020094040A1 (zh) | 2020-05-14 |
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