CN113166792B - 一种用于从含内毒素源或潜在含内毒素源中去掉或去除内毒素的方法 - Google Patents

一种用于从含内毒素源或潜在含内毒素源中去掉或去除内毒素的方法 Download PDF

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Publication number
CN113166792B
CN113166792B CN201980082566.4A CN201980082566A CN113166792B CN 113166792 B CN113166792 B CN 113166792B CN 201980082566 A CN201980082566 A CN 201980082566A CN 113166792 B CN113166792 B CN 113166792B
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endotoxin
solid phase
endotoxins
phage
tren
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Chinese (zh)
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CN113166792A (zh
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P·S·加农
L·雷布拉
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Sedolis Biya Separation Co ltd
Sartorius Bia Separations doo
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Sedolis Biya Separation Co ltd
Bia Separations doo
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CN201980082566.4A 2018-12-14 2019-12-16 一种用于从含内毒素源或潜在含内毒素源中去掉或去除内毒素的方法 Active CN113166792B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18212639.1 2018-12-14
EP18212639 2018-12-14
PCT/EP2019/085401 WO2020120801A1 (en) 2018-12-14 2019-12-16 A method for depletion or removal of endotoxin from an endotoxin-containing source or potentially endotoxin-containing source

Publications (2)

Publication Number Publication Date
CN113166792A CN113166792A (zh) 2021-07-23
CN113166792B true CN113166792B (zh) 2024-06-25

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CN201980082566.4A Active CN113166792B (zh) 2018-12-14 2019-12-16 一种用于从含内毒素源或潜在含内毒素源中去掉或去除内毒素的方法

Country Status (5)

Country Link
US (1) US12492383B2 (enExample)
EP (1) EP3894579A1 (enExample)
JP (2) JP7650811B2 (enExample)
CN (1) CN113166792B (enExample)
WO (1) WO2020120801A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220069102A (ko) 2019-10-01 2022-05-26 어댑티브 페이지 테라퓨틱스, 인크. 박테리오파지 입자의 정제 방법
SI3995196T1 (sl) * 2020-11-04 2023-10-30 Sartorius Bia Separations D.O.O. Postopek čiščenja kompleksnih sklopov, zaprtih v lipidno membrano
CN114230641B (zh) * 2021-12-29 2024-03-26 内蒙古必威安泰生物科技有限公司 一种去除口蹄疫抗原液中内毒素的方法
CN115161308A (zh) * 2022-06-13 2022-10-11 江苏丰华生物制药有限公司 一种注射用替奈普酶上清液的纯化方法
WO2025111368A1 (en) * 2023-11-20 2025-05-30 San Diego State Unversity (Sdsu) Foundation, Dba San Diego State University Research Foundation Methods for purifying bacteriophage and products of manufacture containing purifyied bacteriophage

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9701558B1 (en) * 2014-02-08 2017-07-11 Mansour S. Bader Methods to treat source water for oil-fields heavy oil and other applications

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2146667A1 (en) 1993-08-18 1995-02-23 Adolf Hoess Lipopolysaccharide-binding and neutralizing peptides
AUPM388494A0 (en) * 1994-02-16 1994-03-10 Csl Limited Process for removing endotoxins
US20020197249A1 (en) * 2001-04-10 2002-12-26 Renal Tech International Devices, systems, and methods for reducing levels of pro-inflammatory or anti-inflammatory stimulators or mediators in blood products
US6365147B1 (en) * 1999-10-13 2002-04-02 New Jersey Institute Of Technology Methods for removing endotoxins from biological solutions using immobilized metal affinity chromatography
CN1137860C (zh) * 2000-12-20 2004-02-11 中国科学院生态环境研究中心 高铁氧化去除水中微藻素的方法
AU2002258734A1 (en) * 2001-04-13 2002-10-28 Wyeth Holdings Corporation Removal of bacterial endotoxin in a protein solution by immobilized metal affinity chromatography
GB0211805D0 (en) 2002-05-22 2002-07-03 Prometic Biosciences Ltd Endotoxin-binding ligands and their use
EP1516188B1 (de) * 2002-06-24 2006-11-15 Profos AG Verfahren zum nachweis und zur entfernung von endotoxin
WO2004106361A2 (en) * 2003-05-30 2004-12-09 Invitrogen Corporation Peptides for metal ion affinity chromatogrpahy
US7294614B2 (en) 2004-10-12 2007-11-13 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
DE102005002969A1 (de) * 2005-01-21 2006-08-03 Profos Ag Verfahren zum Nachweis und zur Entfernung von Endotoxin
GB0621452D0 (en) 2006-10-27 2006-12-06 Ucl Business Plc Therapy for liver disease
US20100004218A1 (en) * 2008-06-20 2010-01-07 Whiteford Jeffery A Bridged polycyclic compound based compositions for renal therapy
WO2013180648A1 (en) * 2012-05-31 2013-12-05 Agency For Science, Technology And Research Methods for use of mixed multifunctional surfaces for reducing aggregate content in protein preparations
US9902942B2 (en) 2013-02-22 2018-02-27 Agency For Science, Technology And Research Chromatographic purification of virus preparations with negatively charged particles
SG11201505202SA (en) 2013-02-22 2015-08-28 Agency Science Tech & Res Materials and methods for removing endotoxins from protein preparations
GB201310151D0 (en) 2013-06-07 2013-07-24 Glaxosmithkline Biolog Sa Novel method
WO2017213588A1 (en) * 2016-06-06 2017-12-14 National University Of Singapore A method and a kit for detecting and/or quantifying endotoxin
CN108743616B (zh) * 2018-05-30 2020-12-25 博瑞生物医药（苏州）股份有限公司 一种超顺磁氧化铁内毒素的去除方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9701558B1 (en) * 2014-02-08 2017-07-11 Mansour S. Bader Methods to treat source water for oil-fields heavy oil and other applications

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
D.P.Blowers.《Handbook of methods and instrumentation in separation science》.Elsevier,2009,第 333-340页. *

Also Published As

Publication number Publication date
JP2022528310A (ja) 2022-06-10
JP2025022878A (ja) 2025-02-14
US12492383B2 (en) 2025-12-09
EP3894579A1 (en) 2021-10-20
JP7650811B2 (ja) 2025-03-25
WO2020120801A1 (en) 2020-06-18
US20220056421A1 (en) 2022-02-24
CN113166792A (zh) 2021-07-23

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