CN113166192A - 产生随机寡核苷酸并确定其序列的方法 - Google Patents

产生随机寡核苷酸并确定其序列的方法 Download PDF

Info

Publication number
CN113166192A
CN113166192A CN201980078595.3A CN201980078595A CN113166192A CN 113166192 A CN113166192 A CN 113166192A CN 201980078595 A CN201980078595 A CN 201980078595A CN 113166192 A CN113166192 A CN 113166192A
Authority
CN
China
Prior art keywords
oligonucleotides
random
oligonucleotide
sequence
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201980078595.3A
Other languages
English (en)
Chinese (zh)
Inventor
斯特林·萨瓦亚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geneinfosec Inc
Original Assignee
Geneinfosec Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneinfosec Inc filed Critical Geneinfosec Inc
Publication of CN113166192A publication Critical patent/CN113166192A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201980078595.3A 2018-11-30 2019-11-30 产生随机寡核苷酸并确定其序列的方法 Pending CN113166192A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862773671P 2018-11-30 2018-11-30
US62/773,671 2018-11-30
PCT/US2019/063890 WO2020113195A1 (en) 2018-11-30 2019-11-30 A method for generating random oligonucleotides and determining their sequence

Publications (1)

Publication Number Publication Date
CN113166192A true CN113166192A (zh) 2021-07-23

Family

ID=70852676

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980078595.3A Pending CN113166192A (zh) 2018-11-30 2019-11-30 产生随机寡核苷酸并确定其序列的方法

Country Status (8)

Country Link
US (1) US20220010306A1 (enExample)
EP (1) EP3887385B1 (enExample)
JP (1) JP2022509868A (enExample)
KR (1) KR20210098491A (enExample)
CN (1) CN113166192A (enExample)
CA (1) CA3120107A1 (enExample)
IL (1) IL283364B1 (enExample)
WO (1) WO2020113195A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024130904A1 (zh) * 2022-12-22 2024-06-27 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021873A2 (en) * 1997-10-28 1999-05-06 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
CN105637097A (zh) * 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
CN105705659A (zh) * 2013-08-28 2016-06-22 赛卢拉研究公司 大规模平行单细胞分析
CN107532207A (zh) * 2015-04-10 2018-01-02 空间转录公司 生物样本的空间区别、多重核酸分析

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
JP4638043B2 (ja) 1998-12-14 2011-02-23 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ポリメラーゼ合成による、単一分子の核酸の配列決定のためのシステムおよび方法
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6545758B1 (en) 2000-08-17 2003-04-08 Perry Sandstrom Microarray detector and synthesizer
US7314712B2 (en) * 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
JP3978187B2 (ja) * 2002-03-19 2007-09-19 富士通株式会社 機能性分子及びその製造方法
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7544793B2 (en) 2005-03-10 2009-06-09 Xialoian Gao Making nucleic acid sequences in parallel and use
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
EP3269824A1 (en) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8367813B2 (en) 2008-11-17 2013-02-05 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
US20150211061A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods for determining a nucleotide sequence
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
US10870872B2 (en) * 2016-04-04 2020-12-22 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis
JP6941680B2 (ja) 2016-10-24 2021-09-29 ジェネインフォセック、インコーポレイテッド 核酸内に存在する情報の秘匿化

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021873A2 (en) * 1997-10-28 1999-05-06 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
CN105637097A (zh) * 2013-08-05 2016-06-01 特韦斯特生物科学公司 从头合成的基因文库
CN105705659A (zh) * 2013-08-28 2016-06-22 赛卢拉研究公司 大规模平行单细胞分析
JP2016533187A (ja) * 2013-08-28 2016-10-27 セルラー リサーチ， インコーポレイテッド 大規模並列単一細胞分析
CN107532207A (zh) * 2015-04-10 2018-01-02 空间转录公司 生物样本的空间区别、多重核酸分析

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HUA-HAO LI, ET AL.: "Evaluation of aptamer specificity with or without primers using clinical samples for C-reactive protein by magnetic-assisted rapid aptamer selection", 《RSC ADVANCES》, pages 42856 - 42865 *
KOSUKE FUJISHIMA, ET AL.: "An overhang-based DNA block shuffling method for creating a customized random library", 《SCIENTIFIC REPORTS》, vol. 5, no. 1, pages 1 - 5, XP055901747, DOI: 10.1038/srep09740 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024130904A1 (zh) * 2022-12-22 2024-06-27 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Also Published As

Publication number Publication date
EP3887385B1 (en) 2024-03-20
WO2020113195A1 (en) 2020-06-04
KR20210098491A (ko) 2021-08-10
CA3120107A1 (en) 2020-06-04
EP3887385A1 (en) 2021-10-06
IL283364B1 (en) 2026-05-01
EP3887385C0 (en) 2024-03-20
JP2022509868A (ja) 2022-01-24
IL283364A (en) 2021-07-29
US20220010306A1 (en) 2022-01-13
EP3887385A4 (en) 2022-08-24

Similar Documents

Publication Publication Date Title
US12590330B2 (en) Methods for indexing samples and sequencing multiple polynucleotide templates
EP1711631B1 (en) Nucleic acid characterisation
JP2022540744A (ja) 核酸の空間情報を検出するためのアレイ及び方法
US20240102091A1 (en) Oligonucleotides
WO2012174337A1 (en) Methods for preparative in vitro cloning
EP2785865A1 (en) Method and kit for characterizing rna in a composition
EP3887385B1 (en) A method for generating random oligonucleotides and determining their sequence
EP2456892B1 (en) Method for sequencing a polynucleotide template
JP7636848B2 (ja) 相補ｄｎａ鎖を増幅する方法
EP3828283A1 (en) An improved sequencing method and kit
EP3798319A1 (en) An improved diagnostic and/or sequencing method and kit
WO2026003843A1 (en) A method and apparatus for sequence-verified gene synthesis and assembly
HK40049321A (en) An improved diagnostic and/or sequencing method and kit
JP2005500824A (ja) 核酸分析
JP2004529663A (ja) Ｈａｐｐｉａｒマッピング

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20210723

WD01 Invention patent application deemed withdrawn after publication