IL283364B1 - Method for producing random oligonucleotides and determining their sequence - Google Patents

Method for producing random oligonucleotides and determining their sequence

Info

Publication number
IL283364B1
IL283364B1 IL283364A IL28336421A IL283364B1 IL 283364 B1 IL283364 B1 IL 283364B1 IL 283364 A IL283364 A IL 283364A IL 28336421 A IL28336421 A IL 28336421A IL 283364 B1 IL283364 B1 IL 283364B1
Authority
IL
Israel
Prior art keywords
sequence
determining
generating random
random oligonucleotides
oligonucleotides
Prior art date
Application number
IL283364A
Other languages
English (en)
Hebrew (he)
Other versions
IL283364A (en
Original Assignee
Geneinfosec Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geneinfosec Inc filed Critical Geneinfosec Inc
Publication of IL283364A publication Critical patent/IL283364A/en
Publication of IL283364B1 publication Critical patent/IL283364B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/02—Phosphorylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/314—Phosphoramidates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IL283364A 2018-11-30 2019-11-30 Method for producing random oligonucleotides and determining their sequence IL283364B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862773671P 2018-11-30 2018-11-30
PCT/US2019/063890 WO2020113195A1 (en) 2018-11-30 2019-11-30 A method for generating random oligonucleotides and determining their sequence

Publications (2)

Publication Number Publication Date
IL283364A IL283364A (en) 2021-07-29
IL283364B1 true IL283364B1 (en) 2026-05-01

Family

ID=70852676

Family Applications (1)

Application Number Title Priority Date Filing Date
IL283364A IL283364B1 (en) 2018-11-30 2019-11-30 Method for producing random oligonucleotides and determining their sequence

Country Status (8)

Country Link
US (1) US20220010306A1 (enExample)
EP (1) EP3887385B1 (enExample)
JP (1) JP2022509868A (enExample)
KR (1) KR20210098491A (enExample)
CN (1) CN113166192A (enExample)
CA (1) CA3120107A1 (enExample)
IL (1) IL283364B1 (enExample)
WO (1) WO2020113195A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115925774B (zh) * 2022-12-22 2024-06-14 百力格生物科技(上海)股份有限公司 合成特异性分子标签的方法及其分子标签接头

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350595B1 (en) * 1997-10-28 2002-02-26 Istituto Di Ricerche Di Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
US20090170074A1 (en) * 1998-12-14 2009-07-02 Li-Cor, Inc. System and method for nucleic acid sequencing by polymerase synthesis
US20160089651A1 (en) * 2013-08-05 2016-03-31 Twist Bioscience Corporation De novo synthesized gene libraries
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4313861B2 (ja) 1997-08-01 2009-08-12 キヤノン株式会社 プローブアレイの製造方法
US6458583B1 (en) 1998-09-09 2002-10-01 Agilent Technologies, Inc. Method and apparatus for making nucleic acid arrays
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
US6545758B1 (en) 2000-08-17 2003-04-08 Perry Sandstrom Microarray detector and synthesizer
US7314712B2 (en) * 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
US7157229B2 (en) 2002-01-31 2007-01-02 Nimblegen Systems, Inc. Prepatterned substrate for optical synthesis of DNA probes
JP3978187B2 (ja) * 2002-03-19 2007-09-19 富士通株式会社 機能性分子及びその製造方法
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
US7544793B2 (en) 2005-03-10 2009-06-09 Xialoian Gao Making nucleic acid sequences in parallel and use
US7572907B2 (en) 2005-04-29 2009-08-11 Agilent Technologies, Inc. Methods and compounds for polynucleotide synthesis
EP3269824A1 (en) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8367813B2 (en) 2008-11-17 2013-02-05 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
US9567645B2 (en) * 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
US20150211061A1 (en) 2014-01-27 2015-07-30 The General Hospital Corporation Methods for determining a nucleotide sequence
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
CN120425026A (zh) * 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
JP6941680B2 (ja) 2016-10-24 2021-09-29 ジェネインフォセック、インコーポレイテッド 核酸内に存在する情報の秘匿化

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350595B1 (en) * 1997-10-28 2002-02-26 Istituto Di Ricerche Di Molecolare P. Angeletti S.P.A. Synthesis of polynucleotides having random sequences
US20090170074A1 (en) * 1998-12-14 2009-07-02 Li-Cor, Inc. System and method for nucleic acid sequencing by polymerase synthesis
US20160089651A1 (en) * 2013-08-05 2016-03-31 Twist Bioscience Corporation De novo synthesized gene libraries
JP2016527313A (ja) * 2013-08-05 2016-09-08 ツイスト バイオサイエンス コーポレーション 新規合成した遺伝子ライブラリ
WO2017176541A1 (en) * 2016-04-04 2017-10-12 President And Fellows Of Harvard College Enzymatic nucleic acid synthesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
VIRNEKAS, B., GE, L., PLUCKTHUN, A., SCHNEIDER, K. C., WELLNHOFER, G. N., & MORONEY, S. E, TRINUCLEOTIDE PHOSPHORAMIDITES: IDEAL REAGENTS FOR THE SYNTHESIS OF MIXED OLIGONUCLEOTIDES FOR RANDOM MUTAGENESIS, 25 December 1994 (1994-12-25) *

Also Published As

Publication number Publication date
EP3887385B1 (en) 2024-03-20
WO2020113195A1 (en) 2020-06-04
KR20210098491A (ko) 2021-08-10
CA3120107A1 (en) 2020-06-04
EP3887385A1 (en) 2021-10-06
EP3887385C0 (en) 2024-03-20
CN113166192A (zh) 2021-07-23
JP2022509868A (ja) 2022-01-24
IL283364A (en) 2021-07-29
US20220010306A1 (en) 2022-01-13
EP3887385A4 (en) 2022-08-24

Similar Documents

Publication Publication Date Title
GB2570367B (en) Dispensing apparatus and method
GB2556419B (en) A hardened random number generator
GB201813478D0 (en) Beverate dispensing apparatus and method
PL3566853T3 (pl) Sposób i urządzenie do formowania saszetki
SG11202006589YA (en) Vending machine and vending method and vending system therefor
ZA201504503B (en) System and method for generating a random number
SG11202101965QA (en) Method for selecting neoepitopes
GB201510740D0 (en) 3D Printing apparatus and a corresponding 3D metal printing method
EP3140818A4 (en) Method for generating random numbers and associated random number generator
HUE067561T2 (hu) Tasakképzõ berendezés és eljárás
PL3432137T3 (pl) Sposób i urządzenie do generowania i pozyskiwania liczb losowych
PL3651226T3 (pl) Sposób formowania saszetki i urządzenie do formowania saszetki
GB201708939D0 (en) Random number generator
GB201416859D0 (en) Method and apparatus for generating a multi-level Pseudo-Random Test
GB2540228B (en) Stratigraphic correlation method and apparatus based on uncertainty
GB2567925B (en) Forming method and apparatus
GB201609508D0 (en) Dispensing method and apparatus
GB201818124D0 (en) Method for generating verifiable random selections
EP3861431B8 (en) Device and method for generating random bit sequences
IL271209A (en) Method and equipment for 3D printing
GB201814977D0 (en) Method and apparatus for refilling a container
IL283364A (en) A method for generating random oligonucleotides and determining their sequence
GB201805793D0 (en) Method and system for generating insight
SG11202011481QA (en) Method and device for rendering lyrics
ZA201805055B (en) A gaming apparatus and associated method