CN113069493A

CN113069493A - Application of oil tea extract in inhibiting sebum secretion

Info

Publication number: CN113069493A
Application number: CN202110391266.3A
Authority: CN
Inventors: 沈建福; 崔心禹; 吴晓琴; 张建军; 芦芳; 王京霞
Original assignee: Jiangshanjian Biotechnology Co ltd; Zhejiang University ZJU
Current assignee: Jiangshanjian Biotechnology Co ltd; Zhejiang University ZJU
Priority date: 2021-04-13
Filing date: 2021-04-13
Publication date: 2021-07-06
Anticipated expiration: 2041-04-13
Also published as: CN113069493B

Abstract

The invention relates to application of a camellia oleifera leaf extract in inhibiting sebum secretion. The invention relates to the oil tea and the sebaceous gland secretion, researches the sebaceous gland secretion inhibition effect of the oil tea leaves, provides a new research direction and a great trend commercialization path for the oil tea leaves, and explores the application of the oil tea leaves in the fields of related foods, health products and medicines and the application of the oil tea leaves as functional ingredients in the daily chemical fields of washing, skin care and the like, thereby expanding the research application range of the oil tea leaves.

Description

Application of oil tea extract in inhibiting sebum secretion

Technical Field

The invention relates to application of a camellia oleifera leaf extract in inhibiting sebum secretion.

Background

The oil tea is leaves of Camellia oleifera (Camellia oleifera Abel), active substances of the Camellia oleifera leaves are extremely rich, but the current theoretical research range is small, and the research is mainly focused on the extraction of polyphenol and flavonoid compounds and the research on antioxidant and anticoagulant functions. The tea-oil tree prunes the plastic and the vast amount of tea-oil that can produce of threshing in the middle of the summer in spring end every year in the young age, does not yet obtain make full use of at present, and the industrial production of tea-oil is used and is in zero development more, and these tea-oil leaves are generally abandoned or are burnt as the wastes material, cause the serious waste of resource, if can the rational utilization tea-oil, then can improve comprehensive utilization and reduce abandonment, and then can improve the economic benefits of tea-oil and promote the development of whole tea-oil industry.

The camellia seed extract is a product obtained by extracting substances left after oil extraction, namely camellia seed cake containing 20-25% of tea saponin, 18-20% of polysaccharide and 15-20% of protein, and the research and industrial application of extracting the tea saponin from the camellia seed cake are mature at present. Tea saponin belongs to triterpenoid saponin, and the main effects of tea saponin are as follows: (1) hemolysis and fish poison functions (2), an antibacterial function (3), an antioxidant function (4), a blood pressure reducing function (5), an insect killing and insect expelling function (6), and cleaning and washing functions. Therefore, the single tea saponin has no report on the functions of inhibiting 5 alpha reductase and removing acnes.

2020110294878 patent of the invention, namely a plant extract composition with multi-target-point scalp oil control and dandruff removal effects, a preparation method thereof and a scalp care composition, teach that the composition consisting of 20-40% of camellia oleifera seed extract, 15-25% of tea bran polysaccharide, 10-30% of citrus peel extract, 10-30% of eucalyptus leaf extract and 1-20% of sophora flavescens extract achieves the multi-target-point oil control and dandruff removal purposes through four different approaches (inhibiting microbial lipase, inhibiting malassezia beta-carbonic anhydrase, inhibiting 5 alpha-reductase in scalp cells and inhibiting propionibacterium acnes).

201610382702X invention the acne-removing repair mask applying the camellia seed meal extracting solution and the preparation method thereof contains the camellia seed oil, the camellia seed meal extracting solution and the allantoin, and the mask is beneficial to the discharge of skin epidermal toxin and the elimination of free radicals, and has the effects of enhancing the immunity of the skin, improving the metabolism of the skin, inhibiting the recurrence of acne and strengthening the function of skin repair.

2011100743354 application of Camellia oleifera Abel extract teaches that: the application of the camellia oleifera abel extract serving as a 5 alpha-reductase inhibitor in preparing a medicament for treating prostatic hyperplasia, a medicament for treating prostatic cancer, a medicament or daily chemical product for treating acne or a medicament or daily chemical product for treating androgen-dependent alopecia.

Disclosure of Invention

The invention aims to provide a new application of an oil tea leaf extract.

In order to solve the technical problems, the invention provides an application of a camellia oleifera leaf extract in inhibiting sebum secretion.

As an improvement of the application of the invention: preparing a medicament for inhibiting sebum secretion.

The usage and dosage of the camellia oleifera leaf extract are as follows:

oral administration: generally 4-9 g/person for one day; smearing: generally, the amount of the active agent is 0.5 to 1.5 mL/person per day.

The invention relates to the oil tea and the sebaceous gland secretion, researches the sebaceous gland secretion inhibition effect of the oil tea leaves, provides a new research direction and a great trend commercialization path for the oil tea leaves, and explores the application of the oil tea leaves in the fields of related foods, health products and medicines and the application of the oil tea leaves as functional ingredients in the daily chemical fields of washing, skin care and the like, thereby expanding the research application range of the oil tea leaves.

Drawings

The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.

FIG. 1 shows the change in the area of sebaceous gland plaques in golden hamster;

FIG. 2 is a graph showing the effect of intragastric COLE on the area of right and left sebaceous gland plaques of golden hamster;

in the context of figure 2, it is shown,

a is the area of the sebaceous gland macula on the left side of the intragastric group, and B is the area of the sebaceous gland macula on the right side of the intragastric group.

^*p<0.05 represents G-LC, G-HC, G-TAN compared with G-CON;

FIG. 3 is a graph showing the effect of externally applied COLE gel on the area of right and left sebaceous gland plaques of golden hamster;

in the context of figure 3, it is shown,

a is the area of the sebaceous gland macula on the left side of the external application group, and B is the area of the sebaceous gland macula on the right side of the external application group;

^#p<0.05 represents A-COLE, A-TAN compared to A-CON;

FIG. 4 is the effect of the drug on the microstructure of the sebaceous gland speckle tissue of golden hamster;

FIG. 5 is a graph showing the effect of oil red O staining on lipid accumulation in sebaceous gland plaques by each drug;

FIG. 6 shows the effect of gavage COLE on the SOD and CAT activities of golden hamster serum and MDA content;

^*p<0.05，^**p<0.01，^***p<0.001 represents G-LC, G-HC, G-TAN compared with G-CON;^#p<0.05；

FIG. 7 shows the effect of gastric perfusion COLE on the SOD activity, CAT activity and MDA content of the local sebaceous gland speckle tissue of golden hamster;

in the context of figure 7 of the drawings,

a is the activity of the SOD of the sebaceous gland tissue, B is the activity of the CAT of the sebaceous gland speckle tissue, and C is the MDA content of the sebaceous gland speckle tissue;

^*p<0.05 represents G-LC, G-HC, G-TAN compared with G-CON;

FIG. 8 shows the effect of externally applied COLE gel on SOD activity, CAT activity and MDA content of sebaceous gland speckle tissue of golden hamster;

in the context of figure 8 of the drawings,

a is the activity of the SOD of the sebaceous gland tissue, B is the activity of the CAT of the sebaceous gland tissue, and C is the MDA content of the sebaceous gland tissue;

^#p<0.05 represents the comparison of A-COLE, A-Tan and A-CON;

FIG. 9 shows the effect of gavage COLE on the levels of triglycerides, cholesterol, and free fatty acids in the serum of golden hamster;

in FIG. 9, A is serum TG content, B is serum TC content, and C is serum NEFA content;

^*p<0.05，^**p<0.01，^***p<0.001 represents G-LC, G-HC, G-TAN compared with G-CON;

FIG. 10 is a graph of the effect of intragastric COLE on the levels of triglyceride, cholesterol, and free fatty acids in sebaceous gland plaque tissue of golden hamster;

in the context of figure 10 of the drawings,

a is the content of TG in the sebaceous gland speckle tissue, B is the content of TC in the sebaceous gland speckle tissue, and C is the content of NEFA in the sebaceous gland speckle tissue;

^*p<0.05 represents G-LC, G-HC, G-TAN compared with G-CON;

FIG. 11 is a graph showing the effect of external application of COLE gel on the triglyceride, cholesterol, and free fatty acid content of sebaceous gland plaques in golden hamster;

in the context of figure 11 of the drawings,

^#p<0.05 represents A-COLE, A-Tan compared to A-CON.

FIG. 12 shows the effect of 50% ethanol extract of Camellia oleifera Abel (G-HFh) on the area of sebaceous gland macula of mice;

in the context of figure 12, it is shown,

^*p<0.05 represents G-HFh, G-TAN compared with G-CON;

FIG. 13 shows the effect of coating 18% Camellia oleifera Abel 50% ethanol extract (A-Fh) on the area of right and left sebaceous gland plaques of golden hamster;

in the context of figure 13, it is shown,

^#p<0.05 represents A-Fh, A-TAN compared to A-CON.

Detailed Description

The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:

example 1: preparation of 50% ethanol camellia oleifera leaf extract (conventional technology):

selecting to obtain oil tea with intact leaves and normal color, drying the oil tea leaves with hot air (drying at low temperature of 50 ℃) until the water content is about 10%, crushing and sieving with 60 meshes, weighing 20g of oil tea powder, adding 50% ethanol solution into the oil tea powder according to the material-liquid ratio of 1:40(W: V, 1g/40ml), stirring and soaking for 3h, performing ultrasonic vibration extraction for 2h (extracting twice), centrifuging at 4000rpm for 10min to separate solid and liquid, performing reduced pressure concentration on supernatant liquid by adopting a rotary evaporation mode to remove organic reagent (ethanol), performing vacuum freeze drying (vacuum degree of 10Pa, -70 ℃ and drying time of 48 hours) on the extract until the extract reaches dry powder (the water content is less than 6.0%), obtaining the oil tea leaf extract (50% ethanol oil tea leaf extract), and naming the extract as COLE.

Example 2 preparation of overcoated gel:

weighing 1g of carbomer 940, placing in 20mL of distilled water, and stirring overnight (about 10-12 hours) by using a magnetic stirrer to obtain a phase I; weighing 8g of glycerol and 12g of propylene glycol, adding into the phase I under the stirring condition of a magnetic stirrer, and stirring uniformly to obtain a phase II;

weighing 18g of 50% ethanol camellia oleifera leaf extract, dissolving in 25mL of absolute ethanol, adding into the phase II, and uniformly stirring to obtain a phase III; weighing 3g of azone and 0.35g of imidazolidinyl urea, adding the mixture into the phase III, uniformly stirring, adjusting the p H value to 6-7 by using a proper amount of triethanolamine, adding distilled water until the total mass is 100g, fully and uniformly stirring by using a magnetic stirrer, and removing bubbles until gel is formed, wherein the gel is named as COLE gel.

Experiment 1: the 50% ethanol Camellia oleifera leaf extract can be used for inhibiting sebum secretion in skin cells by oral administration and topical application.

The method comprises the following steps:

golden hamster: SPF grade healthy LVG Syrian Golden hamsters (LVG Golden Syrian hamsters)49 weeks old (Golden hamsters 6 weeks sexual maturity, 8 weeks somatic maturity), weighing 120 ± 20 g: provided by Beijing Wittingle laboratory animal technology Co., Ltd, the certification number is SCXK (Jing) 2016-;

golden hamster is the ideal animal model of research medicine to the influence of sebaceous gland, and its back has 2 natural sebaceous gland plaques, does not need artificial processing to make the mould.

The improvement effect on the sebaceous gland plaques of the golden-yellow hamster is respectively researched by adopting two administration modes of 'intragastric' COLE and 'external application' COLE gel.

1. Grouping of gavage experimental animals

28 male SPF-grade golden yellow hamster 7 weeks old (golden yellow hamster 6 weeks sexually mature, 8 weeks somatic mature), 100 + -20 g in weight were purchased from Beijing Wintolite laboratory animal technologies, Inc. The 28 golden hamster were randomly divided into 4 groups of 7 mice each (2 cages per group). According to the experimental requirements, the test is divided into a 0.5% CMC-NA gavage group (blank control group), a COLE low-dose gavage group, a COLE high-dose gavage group and a tanshinone capsule gavage group (positive control group).

Description of the drawings: CMC-NA is sodium carboxymethyl cellulose, tanshinone capsule is obtained by conventional market purchase mode.

TABLE 1 grouping of Experimental animals and dosing

2. Grouping of external-applied experimental animals

21 male golden yellow hamster of SPF grade identical to the above conditions were randomly divided into 3 groups of 7 mice each (3 cages fed per group). The gel is divided into a blank gel external application group (blank control group), a COLE gel external application group and a kecuocryptone gel external application group (positive control group) according to the requirements of the external application experiment.

Blank gel (matrix gel): the use of 50% ethanolic camellia oleifera leaf extract of example 2 was eliminated, i.e. the amount was 0, and the equivalent amount of water was substituted for COLE, the remainder being identical to example 2.

Kecuocryptone gel: are commercially available.

TABLE 2 Experimental groups and dosing

3. Animal feeding and handling

The purchased golden pheasants are placed in an artificial environment with 12 hours of illumination and 12 hours at night and kept at a constant temperature of 20 ℃ and fed with standard word materials, after adaptive feeding for 1 week, adverse reactions do not occur, and people with normal diet, drinking water and activities are brought into an experiment. After the experiment, the food is placed in cages, and the food is eaten for 2 times a day and is drunk freely.

Before the experiment, the hairs on the two sides of the back of the golden hamster are shaved off by using an electric shaver, so that sebaceous gland spots on the surface of the back skin are clearly exposed, pentobarbital sodium is injected into the golden hamster of each experiment group for anesthesia, under the assistance of strong light, the maximum transverse Diameter (DT) and the maximum longitudinal Diameter (DL) of the sebaceous gland spots on the left side and the right side of the back of each golden hamster are measured by using a vernier caliper, the area of the sebaceous gland spots is calculated, and the photographing record is carried out.

S＝DT×DL(mm²)

Wherein S represents the area of sebaceous gland plaques, and DT and DL represent the maximum transverse diameter and the maximum longitudinal diameter respectively.

After entering the official test, the gavage group experiment is performed with the gavage administration of 8:30 every morning for 4 weeks, 1 time every day, and the gavage amount is 0.02 mL/g. The preparation method comprises diluting ColE lyophilized powder and tanshinone powder with 0.5% CMC-Na solution (0.5% sodium carboxymethylcellulose solution) to obtain suspension, and intragastrically administering the blank control group with 0.5% CMC-Na solution.

For the external application group experiment, external application is carried out every morning and afternoon for 4 weeks and 2 times every day, and the external application amount is 0.6 mL/d.

The general conditions (whether the activity is normal, the condition of diarrhea, the food intake, the stool form and the like) of the rats and the conditions of sebaceous gland plaques (the application part has no desquamation, hyperkeratosis, redness, skin cracks or other adverse reactions) are observed and recorded every day, and the judgment of the observed results is recorded according to the skin irritation response scoring standard of the preclinical guidelines of new drugs. The body weight and sebaceous gland plaque area data of golden hamster of each experimental group were weighed and recorded weekly, and newly growing hair on the back was cleaned in time.

And (4) judging the standard: GB17149.3-1997 cosmetic acne diagnosis standard and treatment principle, and DB37T3744-2019 cosmetic adverse reaction skin damage form judgment standard and treatment.

The golden hamster is used as an ideal animal model for researching the influence of the medicament on sebaceous glands, and the influence of the medicament on the sebaceous glands of the golden hamster is researched by observing the apparent forms of sebaceous gland plaques on two sides of the back of the golden hamster and determining and comparing the area size of the sebaceous gland plaques. The results are shown in FIG. 1; shows the change of sebaceous gland plaques of golden yellow hamster in different administration modes of 0d, 14d and 28d (COLE and COLE gel).

The size and color of sebaceous gland plaques of the 0.5% CMC-Na intragastric group (i.e., blank control group, G-CON) and blank gel overcoated group (A-CON) did not significantly change during the experimental period, where skin pores are large and clearly visible, containing crude pigmented (black) hairs, and gray halos around part of sebaceous gland plaques, which are the result of melanin secretion by melanocytes.

Compared with the group of gavage with 0.5% CMC-Na, it can be seen that the sebaceous gland macula becomes shallow in the group of low-dose COLE gavage (G-LC), high-dose COLE gavage (G-HC) and tanshinone capsule gavage (G-Tan, i.e., positive control group), the junction between the sebaceous gland macula and the surrounding skin gradually blurs, and the range of macula protrusion is reduced.

The sebaceous gland plaques of the COLE gel-coated group (A-COLE) and the Claptocryptine gel-coated group (A-KCYT) also showed a tendency to lighten with the time of administration, compared to the blank gel-coated group (A-CON).

The change results of the sebaceous gland macula area of golden-yellow hamster (average results in each group) are shown in fig. 2 and fig. 3, the adaptive feeding is not administrated in the zeroth week, and the sebaceous gland macula of each group has no significant difference (p is more than 0.05). The administration was started from the first week, the change of the area of the sebaceous gland plaques in the left and right sides of the first week and the second week of the gavage group was still not obvious (p is more than 0.05), the drug action was revealed from the third week and the fourth week, and compared with the blank gavage group (G-CON), the low and high dose of COLE and the positive drug both significantly reduced the area of the sebaceous gland plaques (p is less than 0.05) as shown in FIG. 2.

For the left sebaceous plaques in the washout group, significant differences (p <0.05) appeared from the second week compared to the blank gel washout group, which continued until the fourth week, the right sebaceous plaques appeared between the groups from the third week, and the area of the COLE sebaceous plaques was significantly smaller than that of the blank group (p < 0.05). That is, the COLE gel from the second week in the overcoated group resulted in a significant reduction in the area of the left sebaceous gland plaques (p <0.05), and a significant reduction in the area of the right sebaceous gland plaques from the third week. As shown in fig. 3.

Indicating that both gastric perfusion and external application can significantly reduce the area of sebaceous gland plaques. That is, it was demonstrated that COLE inhibits the development of sebaceous glands, and thus inhibits sebum secretion.

Experiment 2, Effect of COLE on histopathology of sebaceous gland plaques of golden hamster

The effect of COLE on the histopathology of sebaceous gland plaques in golden hamsters was observed using HE staining (see FIG. 4).

The sebaceous gland is a vesicular gland, which is composed of one or several vesicular acini and a common short duct. The periacinar is a juvenile cell which has active division ability and can generate new glandular cells. The newly formed gland cells grow larger and move toward the center of the acinus, secreting more and more droplets of fat in the cytoplasm.

As shown in FIG. 4, the microstructure of the local sebaceous gland speckle tissue of golden hamster in each administration group was changed. In fig. 4: G-CON: gavage placebo; G-LC: COLE low dose intragastric group; G-HC: COLE high dose gavage group; G-Tan: tanshinone capsule for gastric lavage (positive control group); A-CON: blank gel overcoating group; A-COLE: COLE gel overcoating group; A-KCYT: kecuocryptone gel topical group.

The sebaceous glands of G-CON and A-CON are distributed in a lobular manner, the glandular lobes are large and full, the number of overlapped lobes is large, the arrangement is compact, the internal saccular acinus is rich and polygonal, and the cells of the surrounding glands are numerous. Compared with G-CON, the low and high dose COLE and tanshinone capsule groups have obviously reduced and loose arrangement of sebaceous acinus, small gland contraction and fusiform, and less cystoid acinus and surrounding gland cells in glands. Compared with A-CON, the COLE gel and the kecuocryptone gel can also obviously reduce the number and the volume of sebaceous follicles, which indicates that the COLE has the effect of inhibiting sebaceous glands of golden-yellow mice by using two administration modes of gastric lavage and external coating.

That is, it was found that the use of COLE in both intragastric and overcoated modes of administration described in experiment 1 resulted in a decrease in the number and arrangement of sebaceous glands in golden hamsters, a decrease in glandular contractions, a decrease in the presence of spindle-like cysts and surrounding glandular cells within the glands. This result is consistent with the reduction in area of the sebaceous gland macula of experiment 1.

Experiment 3, Effect of COLE on sebaceous gland plaque lipid secretion in golden hamster

The effect of COLE on the lipid secretion of sebaceous gland plaques of golden hamster was observed by oil red O staining, as shown in FIG. 5.

The lipid secretion of sebaceous glands of each group was observed by means of oil red O staining. As shown in FIG. 5, a large number of red globose lipid droplets are clearly visible in the cytoplasm of the endothelial lipid gland cell of the sebaceous plaque tissue of golden hamster in the G-CON and A-CON groups, and the red globose lipid droplets are fused into a sheet shape and are secreted vigorously. Compared with the G-CON group, the accumulation of orange lipid droplets around the acinus of the G-LC, G-HC and G-Tan groups is obviously reduced. The A-COLE and A-KCYT groups also showed a decrease in sebum secretion compared to the A-CON group.

That is, from fig. 5, it can be seen that: the intragastric COLE and the external coating COLE gel can reduce the accumulation of orange lipid drops around sebaceous gland acinus of golden-yellow hamster, and reduce the lipid secretion level in sebaceous gland speckle tissues.

Experiment 4, COLE influence on Biochemical indexes of golden hamster body and sebaceous gland speckle tissue

The influence of COLE on oxidative stress (SOD, CAT activity and MDA content) and lipid secretion level (including triglyceride, cholesterol and free fatty acid) of golden pheasant and sebaceous gland speckle tissue is researched by adopting enzyme-linked immunosorbent assay and other methods.

(1) Oxidative stress index

For the golden hamster of the gavage group, the results of the in vivo oxidative stress indexes show that for low and high dose COLE, the SOD activity in serum can be obviously improved (p <0.05, p <0.01), the CAT activity is obviously increased (p <0.05), and the MDA content corresponding to the oxidative stress products is extremely obviously reduced (p <0.001), as shown in figure 6. The SOD activity and CAT activity of local sebaceous gland speckle tissues of golden-yellow mice in the gastric lavage group are increased, and the MDA content of the G-HC group is remarkably reduced (p is less than 0.05), as shown in figure 7. It is shown that gavage COLE can significantly reduce the severity of free radical attack on the interior of the body.

For the external application group, the COLE gel enables the SOD activity of the local sebaceous gland speckle tissues of golden hamster to be remarkably increased (p is less than 0.05), the MDA content is remarkably reduced (p is less than 0.05), the CAT activity has an increasing trend and is better than that of the positive drug, which shows that the COLE gel has outstanding capability of resisting oxidative stress and can play a role in protecting the skin, as shown in figure 8.

(2) Lipid secretion index:

the low and high dose COLE can significantly reduce the TG content (p <0.001), the NEFA content (p <0.001) and the TC content (p <0.05) in the serum of the golden hamster in the gavage group, as shown in FIG. 9. Meanwhile, TG (p is less than 0.001) and NEFA (p is less than 0.001) in sebaceous gland speckle tissues of golden-yellow mice can be remarkably reduced. The effect of gavage COLE was comparable to that of the positive control. The skin surface cholesterol level was low, about 2% of sebum, and the TC level, although slightly decreasing, did not change significantly (p > 0.05) as shown in FIG. 10. The index result shows that the intragastric COLE can not only reduce the content of serum lipid components in vivo, but also reduce the content of sebaceous gland sebum of golden hamster, and the result trends are basically consistent.

For the experimental group of the external application mode, the content of TG and NEFA in the sebaceous gland speckle tissues of golden yellow mice of the COLE gel external application group is greatly reduced (p is less than 0.01, and p is less than 0.01), and the results of the TG and the NEFA are equivalent. This indicates that the intervention of COLE gel can also significantly reduce the sebum content in sebaceous gland plaques of golden hamster, and control the secretion of oil, as shown in FIG. 11.

And (4) conclusion: the 50% ethanol camellia oleifera leaf extract has good oxidation resistance, can reduce the area of golden hamster sebaceous gland spots, reduce the number of sebaceous glands, can reduce the sebum content in golden hamster sebaceous gland spot tissues, and controls the secretion of grease. Can be applied to the fields of cosmetics, washing and caring products, medical care and the like with oil control and sebum secretion inhibition requirements (or functions), and has wide market prospect.

Comparative experiment,

The camellia oleifera leaves in example 1 were changed into camellia oleifera abel, according to the method described in example 1, to obtain the corresponding camellia oleifera abel extract (50% ethanolic camellia oleifera abel extract). The camellia oleifera abel extract was substituted for the 50% ethanolic camellia oleifera abel leaf extract in example 2, thereby obtaining the corresponding camellia oleifera abel gel.

The contents of flavone and polyphenol in the camellia oleifera leaf extract and the camellia oleifera fruit extract are respectively measured according to a conventional sodium nitrite-aluminum nitrate colorimetric method and a Folin-Ciocalteu method, and the obtained results are shown in the following table 3:

TABLE 3

The oil tea camellia oleifera abel extract (Fh) is used for replacing an oil tea camellia oleifera abel leaf extract, an experiment for inhibiting sebum secretion is carried out according to the experiment method, an experiment group for perfusing 50% of the oil tea camellia oleifera abel 50% ethanol extract is named as G-HFh, and an experiment group for externally coating 18% of the oil tea camellia oleifera abel 50% ethanol extract is named as A-Fh.

Results 1, effect of gavage 2700 mg/kg. d of 50% ethanol extract of Camellia oleifera Abel (G-HFh) on the area of right and left sebaceous gland plaques of golden hamster:

compared with the blank control G-CON, the area of the left sebaceous gland plaque of the positive control G-TAN is reduced remarkably from the fourth week (p <0.05), and the area of the right sebaceous gland plaque is reduced remarkably from the third week (p < 0.05). Compared with the blank control G-CON, the area of sebaceous gland plaques on both sides of the golden yellow hamster tends to be reduced from the third week until the end of the 4-week experiment, and the result has no significant difference (p is more than 0.05). As shown in fig. 12.

Results 2, the effect of externally applying 18% Camellia oleifera Abel 50% ethanol extract (A-Fh) on the area of right and left sebaceous gland plaques of golden hamster

The experiment of externally coating 18 percent of camellia oleifera abel 50 percent ethanol extract (A-Fh) shows that the area of sebaceous gland plaques on the left and right sides of golden yellow hamster in the positive control A-KCYT group is obviously reduced from the second week and the third week respectively (p is less than 0.05) compared with the blank group A-CON. Compared with the blank group A-CON, the areas of the sebaceous gland plaques on the left and right sides of the golden hamster coated with the 18% camellia oleifera Abel 50% ethanol extract A-Fh group show a trend of reduction from the second week and the first week respectively until the results of the four-week experiment, but the results have no significant difference (p is more than 0.05), as shown in FIG. 13.

The experimental results of the administration mode of either intragastric administration or external application show that the 50% ethanol extract of the camellia oleifera abel has a slight reduction tendency on sebaceous gland plaques of golden hamster, but the results have no significant difference (p is more than 0.05).

Results 3, gavage 2700 mg/kg. d oil tea cattail 50% ethanol extract (G-HFh) has influence on the content of triglyceride, cholesterol and free fatty acid in the sebaceous gland macula tissue of golden hamsters; as in table 4.

TABLE 4 triglyceride, cholesterol, free fatty acid content in the sebaceous gland speckle tissue of golden hamster (gavage)

Note:^*p<0.05，^***p<0.001 represents G-HFh, G-TAN compared with G-CON

The results in table 4 show that: the positive control group G-TAN can remarkably reduce the TG content (p is less than 0.001) and the NEFA content (p is less than 0.001) in the sebaceous gland speckle tissues of golden yellow mice, and the camellia oleifera abel extract G-HFh can also remarkably reduce the TG content and the NEFA content (p is less than 0.05 and p is less than 0.05), but the effect is far lower than that of the G-TAN. G-HFh had no significant effect on TC content in sebaceous plaques (p > 0.05).

The result 4 shows that the externally applied 50% ethanol extract gel of the camellia oleifera abel has influence on the contents of triglyceride, cholesterol and free fatty acid in the sebaceous gland speckle tissues of the golden-yellow hamster; as in table 5.

TABLE 5 triglyceride, cholesterol, free fatty acid content in the sebaceous gland speckle tissue of golden hamster (external application)

Note:^**p<0.01 denotes A-Fh, A-KCYT compared with A-CON

The results in Table 5 show that the positive control group A-KCYT can obviously reduce the TG content (p <0.01) and the NEFA content (p <0.01) in the sebaceous gland speckle tissues of golden yellow mice, and the Camellia oleifera Abel extract A-Fh shows the trend of reducing the TG content and the NEFA content in the sebaceous gland speckle tissues, but the results have no obvious difference (p > 0.05). A-Fh has no significant effect on TC content in sebaceous gland speckle tissues (p is more than 0.05). The results of this experiment demonstrate that the extract a-Fh of camellia oleifera abel has a tendency to inhibit the lipid secretion of sebaceous glands, but the results are not significant and are consistent with the conclusions of result 2.

Finally, it is also noted that the above-mentioned lists merely illustrate a few specific embodiments of the invention. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.

Claims

1. Use of an extract of Camellia sinensis leaf for inhibiting sebum secretion is provided.

2. Use according to claim 1, characterized in that: preparing a medicament for inhibiting sebum secretion.