CN113005108B - 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 - Google Patents

用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 Download PDF

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CN113005108B
CN113005108B CN202110295229.2A CN202110295229A CN113005108B CN 113005108 B CN113005108 B CN 113005108B CN 202110295229 A CN202110295229 A CN 202110295229A CN 113005108 B CN113005108 B CN 113005108B
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CN113005108A (zh
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克里斯蒂安·格勒克纳
阿米尔阿里·基亚
艾琳·博马蒂
莫利·何
海缨·李·格鲁内瓦德
斯科特·库尔斯腾
特瑞纳·法耶·奥索斯普拉罗普
达林·哈斯金斯
约书亚·伯吉斯
阿奴巴玛·康纳
丹尼尔·施凌曼
拉梅什·威德亚纳森
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    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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CN202110295229.2A 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶 Active CN113005108B (zh)

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US201461979871P 2014-04-15 2014-04-15
US61/979,871 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US62/062,006 2014-10-09
US201462080882P 2014-11-17 2014-11-17
US62/080,882 2014-11-17
CN201580027103.XA CN106507677B (zh) 2014-04-15 2015-04-15 用于改进插入序列偏倚和增加dna输入耐受性的修饰的转座酶
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

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