CN112972604A - Blood fat reducing traditional Chinese medicine decoction pieces and preparation method thereof - Google Patents

Abstract

The invention relates to the technical field of hyperlipidemia treatment, in particular to a traditional Chinese medicine decoction piece for reducing blood fat and a preparation method thereof. The blood fat reducing traditional Chinese medicine decoction pieces are prepared from the following raw materials in parts by weight: 10-15 parts of ginkgo leaves, 10-15 parts of safflower, 6-8 parts of rhizoma alismatis, 8-12 parts of radix bupleuri, 15-20 parts of cortex lycii radicis, 3-6 parts of parasitic loranthus, 6-10 parts of wolfberry fruits, 10-15 parts of corn embryos, 3-6 parts of hawthorn fruits, 0.5-1 part of adsorbent and 0.01-0.02 part of solubilizer. In the preparation process, the raw materials are fermented by the microbial inoculum A and the microbial inoculum B, so that the interaction and the conversion of active ingredients in the raw materials can be promoted in the fermentation process, and the bioactive ingredients of the Chinese herbal medicine extract are improved; the prepared traditional Chinese medicine decoction pieces have good blood fat reducing effect, and simultaneously have high extract content and good organism absorption effect.

Description

Blood fat reducing traditional Chinese medicine decoction pieces and preparation method thereof

Technical Field

The invention relates to the technical field of hyperlipidemia treatment, in particular to a traditional Chinese medicine decoction piece for reducing blood fat and a preparation method thereof.

Background

Hyperlipidemia is a common name of Hyperlipidemia (Hyperlipidemia), also called Hyperlipoproteinemia (Hyperlipoproteinemia), refers to the condition of high blood lipid level, and is the most common form of Dyslipidemia (Dyslipidemia). The disorder is divided into "primary" and "secondary subtypes". Primary hyperlipidemia often results from genetic causes (e.g., mutations in receptor proteins), while secondary hyperlipidemia results from other causes, such as diabetes. Lipid and lipoprotein abnormalities are more common in the general population, and in addition, certain forms of hyperlipidemia may induce Acute pancreatitis (Acute pancreatitis).

The clinical manifestations of hyperlipidemia are mainly yellow tumors caused by intradermal deposition of lipids and arteriosclerosis caused by endothelial deposition of lipids in blood vessels. The high blood lipid content mainly shows that the total cholesterol content (TC), the triglyceride content (TG) and the low-density cholesterol (LDL-C) in a body are increased, and the high blood lipid content can cause various diseases.

In the aspect of the medicine for treating hyperlipidemia, western medicines and traditional Chinese medicines are mainly used. Among the traditional Chinese medicines, the medlar, the pseudo-ginseng, the gynostemma pentaphylla and the like are researched more, and the traditional Chinese medicine also has a certain effect of reducing blood fat. The flavone, triterpene and polysaccharide in the traditional Chinese medicine components may have the function of reducing blood fat. And some patients still have poor curative effect on the common hypolipidemic drugs or can not be applied due to side effects of the hypolipidemic drugs; and is inconvenient to carry. The traditional Chinese medicine decoction pieces are common traditional Chinese medicine preparation modes in recent years, have the advantages of convenience, rapidness, convenience in carrying and the like, but the traditional Chinese medicine decoction pieces for reducing blood fat in the market are mostly prepared by directly pressing traditional Chinese medicine powder, are low in leaching rate and absorption rate and not ideal in curative effect, and only can play a role in assisting treatment of western medicines during treatment.

Disclosure of Invention

The invention aims to provide a blood fat reducing traditional Chinese medicine decoction piece and a preparation method thereof, and the preparation method is realized by the following technical scheme:

a traditional Chinese medicine decoction piece for reducing blood fat is prepared from the following raw materials in parts by weight: 10-15 parts of ginkgo leaves, 10-15 parts of safflower, 6-8 parts of rhizoma alismatis, 8-12 parts of radix bupleuri, 15-20 parts of cortex lycii radicis, 3-6 parts of parasitic loranthus, 6-10 parts of wolfberry fruits, 10-15 parts of corn embryos, 3-6 parts of hawthorn fruits, 0.5-1 part of adsorbent and 0.01-0.02 part of solubilizer.

Preferably, the blood fat reducing traditional Chinese medicine decoction pieces are prepared from the following raw materials in parts by weight: 12 parts of ginkgo leaves, 10 parts of safflower, 6 parts of rhizoma alismatis, 10 parts of radix bupleuri, 18 parts of cortex lycii radicis, 5 parts of parasitic loranthus, 8 parts of wolfberry fruit, 12 parts of corn germ, 4 parts of hawthorn, 0.6 part of adsorbent and 0.012 part of solubilizer.

Preferably, the adsorbent is chitosan.

Preferably, the solubilizer consists of rhamnolipid and calcium chloride in a mass ratio of 3-4: 1.

Preferably, the preparation method of the blood fat reducing traditional Chinese medicine decoction pieces comprises the following steps:

(1) pulverizing folium Ginkgo, Alismatis rhizoma, bupleuri radix, cortex Lycii, and herba Taxilli to 40-60 mesh, adjusting water content to 60-70%, parching at 60-70 deg.C to 30-40%, cooling, and making into Chinese medicinal powder;

(2) adding 0.01-0.02 wt% of microbial inoculum A into the Chinese medicinal powder, stirring, fermenting at 25-30 deg.C for 3-4 days, and sterilizing to obtain primary fermented product; adding 0.03-0.04% of microbial inoculum B into the primary fermentation product, fermenting at 22-25 deg.C for 1-2 days, and sterilizing to obtain primary fermentation product; preparing a fermented product;

(3) adding 80% ethanol 8-10 times of the fermented product, reflux extracting at 60-70 deg.C for 25-30min, filtering, and recovering ethanol from the filtrate under reduced pressure to obtain extractive solution;

(4) pulverizing Carthami flos, fructus crataegi, semen Maydis, and fructus Lycii, mixing, adding 15 times of water, and decocting at 60-70 deg.C for 1.5-2 hr to obtain paste;

(5) mixing the paste with the extractive solution, adsorbent and solubilizer, and homogenizing to obtain mixture; vacuum freeze drying the mixture, pulverizing to obtain medicinal powder; finally, tabletting and pressing to obtain tablets with the diameter of 2-2.5cm and the thickness of 0.2-0.3 mm; making into Chinese medicinal decoction pieces.

Preferably, the microbial inoculum A is aspergillus.

Preferably, the microbial inoculum B is bacillus cereus.

The invention has the beneficial effects that: the ginkgo leaf, the alisma orientale, the bupleurum, the cortex lycii radicis and the loranthus parasiticus are used in combination, so that the effects of inhibiting exogenous cholesterol absorbed by intestinal tracts, clearing liver and draining, and promoting blood circulation to remove blood stasis are achieved, and the good blood fat reducing effect is achieved. The combination of the flower, the hawthorn, the corn germ and the medlar has the effects of invigorating spleen and tonifying qi, promoting qi circulation and harmonizing stomach, and removing food retention and removing food stagnation, and can improve the absorption capacity of the intestines and stomach of the organism on the effective components in the tea. The adsorbent and the solubilizer can keep good stability of the effective components in the decoction pieces, improve the biocompatibility of the decoction pieces, further promote the absorption of the decoction pieces and have no damage to body cells.

In the preparation process, the raw materials are fermented by the microbial inoculum A and the microbial inoculum B, so that the interaction and the conversion of active ingredients in the raw materials can be promoted in the fermentation process, and the bioactive ingredients of the Chinese herbal medicine extract are improved; the prepared traditional Chinese medicine decoction pieces have good blood fat reducing effect, and simultaneously have high extract content and good organism absorption effect.

Detailed Description

The technical solution of the present invention is further defined below with reference to the specific embodiments, but the scope of the claims is not limited to the description.

Example 1

The components are as follows: 10kg of ginkgo leaves, 10kg of safflower, 6kg of rhizoma alismatis, 8kg of radix bupleuri, 15kg of cortex lycii radicis, 3kg of parasitic loranthus, 6kg of medlar, 10kg of corn embryos, 3kg of hawthorn, 0.5kg of chitosan and 0.01kg of solubilizer. The solubilizer consists of rhamnolipid and calcium chloride according to the mass ratio of 3: 1.

The preparation method of the blood fat reducing traditional Chinese medicine decoction pieces comprises the following steps:

(1) pulverizing folium Ginkgo, Alismatis rhizoma, bupleuri radix, cortex Lycii, and herba Taxilli to 40-60 mesh, adjusting water content to 60-70%, parching at 60-70 deg.C to 30-40%, cooling, and making into Chinese medicinal powder;

(2) adding Aspergillus 0.01 wt% into the Chinese medicinal powder, stirring, fermenting at 25-30 deg.C for 3 days, and sterilizing to obtain primary fermented product; adding Bacillus cereus 0.03 wt% into the primary fermented product, fermenting at 22-25 deg.C for 1d, and sterilizing to obtain primary fermented product; preparing a fermented product;

(3) adding 8 times of 80% ethanol into the fermented product, reflux extracting at 60-70 deg.C for 25min, filtering, and recovering ethanol from the filtrate under reduced pressure to obtain extractive solution;

(4) pulverizing Carthami flos, fructus crataegi, semen Maydis, and fructus Lycii, mixing, adding 15 times of water, and decocting at 60-70 deg.C for 1.5h to obtain paste;

(5) mixing the paste with the extractive solution, adsorbent and solubilizer, and homogenizing to obtain mixture; vacuum freeze drying the mixture, pulverizing to obtain medicinal powder; finally, tabletting and pressing to obtain tablets with the diameter of 2-2.5cm and the thickness of 0.2-0.3 mm; making into Chinese medicinal decoction pieces.

Example 2

The components are as follows: 12kg of ginkgo leaves, 10kg of safflower, 6kg of rhizoma alismatis, 10kg of radix bupleuri, 18kg of cortex lycii radicis, 5kg of parasitic loranthus, 8kg of medlar, 12kg of corn embryos, 4kg of hawthorn, 0.6kg of chitosan and 0.012kg of solubilizer. The solubilizer consists of rhamnolipid and calcium chloride according to the mass ratio of 3-4: 1.

The preparation method of the blood fat reducing traditional Chinese medicine decoction pieces comprises the following steps:

(1) pulverizing folium Ginkgo, Alismatis rhizoma, bupleuri radix, cortex Lycii, and herba Taxilli to 40-60 mesh, adjusting water content to 60-70%, parching at 60-70 deg.C to 30-40%, cooling, and making into Chinese medicinal powder;

(2) adding Aspergillus 0.015% of the weight of the powder into the powder, stirring, fermenting at 25-30 deg.C for 4 days, and sterilizing to obtain primary fermented product; adding Bacillus cereus 0.032 wt% into the primary fermented product, fermenting at 22-25 deg.C for 1d, and sterilizing to obtain primary fermented product; preparing a fermented product;

(3) adding 10 times of 80% ethanol into the fermented product, reflux extracting at 60-70 deg.C for 28min, filtering, and recovering ethanol from the filtrate under reduced pressure to obtain extractive solution;

(4) pulverizing Carthami flos, fructus crataegi, semen Maydis, and fructus Lycii, mixing, adding 15 times of water, and decocting at 60-70 deg.C for 1.8 hr to obtain paste;

(5) mixing the paste with the extractive solution, adsorbent and solubilizer, and homogenizing to obtain mixture; vacuum freeze drying the mixture, pulverizing to obtain medicinal powder; finally, tabletting and pressing to obtain tablets with the diameter of 2-2.5cm and the thickness of 0.2-0.3 mm; making into Chinese medicinal decoction pieces.

Example 3

The components are as follows: 15kg of ginkgo leaves, 15kg of safflower, 8kg of rhizoma alismatis, 12kg of radix bupleuri, 20kg of cortex lycii radicis, 6kg of parasitic loranthus, 10kg of medlar, 15kg of corn embryos, 6kg of hawthorn, 1kg of chitosan and 0.02kg of solubilizer. The solubilizer consists of rhamnolipid and calcium chloride according to the mass ratio of 4: 1.

The preparation method of the blood fat reducing traditional Chinese medicine decoction pieces comprises the following steps:

(1) pulverizing folium Ginkgo, Alismatis rhizoma, bupleuri radix, cortex Lycii, and herba Taxilli to 40-60 mesh, adjusting water content to 60-70%, parching at 60-70 deg.C to 30-40%, cooling, and making into Chinese medicinal powder;

(2) adding Aspergillus 0.02 wt% into the Chinese medicinal powder, stirring, fermenting at 25-30 deg.C for 4 days, and sterilizing to obtain primary fermented product; adding Bacillus cereus 0.04 wt% into the primary fermented product, fermenting at 22-25 deg.C for 2d, and sterilizing to obtain primary fermented product; preparing a fermented product;

(3) adding 10 times of 80% ethanol into the fermented product, reflux extracting at 60-70 deg.C for 30min, filtering, and recovering ethanol from the filtrate under reduced pressure to obtain extractive solution;

(4) pulverizing Carthami flos, fructus crataegi, semen Maydis, and fructus Lycii, mixing, adding 15 times of water, and decocting at 60-70 deg.C for 2 hr to obtain paste;

(5) mixing the paste with the extractive solution, adsorbent and solubilizer, and homogenizing to obtain mixture; vacuum freeze drying the mixture, pulverizing to obtain medicinal powder; finally, tabletting and pressing to obtain tablets with the diameter of 2-2.5cm and the thickness of 0.2-0.3 mm; making into Chinese medicinal decoction pieces.

And (3) testing the blood fat reducing effect: experiment design: 40 primary hyperlipemia SD rats are female in a clean grade, aged for 10 weeks, and bred in a single cage with the weight of 180-. The experimental rats were randomly divided into 4 groups of 10 animals each, after acclimation for 7 days, and the experiments were performed. Respectively, blank control group (gavage equivalent physiological saline), experiment 1-3 groups respectively gavage the blood lipid reducing decoction pieces of examples 1-3, the gavage amount is 10mg/kg, and the gavage is performed at regular time every day. The SD rat is continuously cultured for 2 months by gastric lavage, when the experiment is finished, the rat fasts without water, cuts off the head to take blood, respectively detects the levels of TG, TC and LDL-C in serum, and records the change of heart rate. The results of the experiment are shown in table 1:

TABLE 1

Group of	With or without abnormality	TG(mmol/L)	TC(mmol/L)	LDL-C(mmol/L)
					Control group	Is free of	3.74±0.36	3.82±0.434	2.57±0.33
Experimental group 1	Is free of	2.12±0.18	2.10±0.24	1.14±0.16
					Experimental group 2	Is free of	2.07±0.14	2.06±0.18	1.12±0.20
Experimental group 3	Is free of	2.18±0.16	2.13±0.21	1.18±0.23

It should be noted that the above examples and test examples are only for further illustration and understanding of the technical solutions of the present invention, and are not to be construed as further limitations of the technical solutions of the present invention, and the invention which does not highlight essential features and significant advances made by those skilled in the art still belongs to the protection scope of the present invention.

Claims (7)

1. The blood fat reducing traditional Chinese medicine decoction piece is characterized by being prepared from the following raw materials in parts by weight: 10-15 parts of ginkgo leaves, 10-15 parts of safflower, 6-8 parts of rhizoma alismatis, 8-12 parts of radix bupleuri, 15-20 parts of cortex lycii radicis, 3-6 parts of parasitic loranthus, 6-10 parts of wolfberry fruits, 10-15 parts of corn embryos, 3-6 parts of hawthorn fruits, 0.5-1 part of adsorbent and 0.01-0.02 part of solubilizer.

2. The blood fat reducing traditional Chinese medicine decoction piece as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 12 parts of ginkgo leaves, 10 parts of safflower, 6 parts of rhizoma alismatis, 10 parts of radix bupleuri, 18 parts of cortex lycii radicis, 5 parts of parasitic loranthus, 8 parts of wolfberry fruit, 12 parts of corn germ, 4 parts of hawthorn, 0.6 part of adsorbent and 0.012 part of solubilizer.

3. The blood lipid reducing traditional Chinese medicine decoction pieces as claimed in claim 1 or 2, wherein the adsorbent is chitosan.

4. The blood lipid reducing traditional Chinese medicine decoction pieces as claimed in claim 1 or 2, wherein the solubilizer consists of rhamnolipid and calcium chloride in a mass ratio of 3-4: 1.

5. The preparation method of the blood fat reducing traditional Chinese medicine decoction pieces as claimed in claim 1 or 2, which comprises the following steps:

(1) pulverizing folium Ginkgo, Alismatis rhizoma, bupleuri radix, cortex Lycii, and herba Taxilli to 40-60 mesh, adjusting water content to 60-70%, parching at 60-70 deg.C to 30-40%, cooling, and making into Chinese medicinal powder;

(2) adding 0.01-0.02 wt% of microbial inoculum A into the Chinese medicinal powder, stirring, fermenting at 25-30 deg.C for 3-4 days, and sterilizing to obtain primary fermented product; adding 0.03-0.04% of microbial inoculum B into the primary fermentation product, fermenting at 22-25 deg.C for 1-2 days, and sterilizing to obtain primary fermentation product; preparing a fermented product;

(3) adding 80% ethanol 8-10 times of the fermented product, reflux extracting at 60-70 deg.C for 25-30min, filtering, and recovering ethanol from the filtrate under reduced pressure to obtain extractive solution;

(4) pulverizing Carthami flos, fructus crataegi, semen Maydis, and fructus Lycii, mixing, adding 15 times of water, and decocting at 60-70 deg.C for 1.5-2 hr to obtain paste;

(5) mixing the paste with the extractive solution, adsorbent and solubilizer, and homogenizing to obtain mixture; vacuum freeze drying the mixture, pulverizing to obtain medicinal powder; finally, tabletting and pressing to obtain tablets with the diameter of 2-2.5cm and the thickness of 0.2-0.3 mm; making into Chinese medicinal decoction pieces.

6. The method for preparing blood fat reducing traditional Chinese medicine decoction pieces according to claim 5, wherein the microbial inoculum A is aspergillus.

7. The method for preparing blood fat reducing traditional Chinese medicine decoction pieces according to claim 5, wherein the microbial agent B is Bacillus cereus.