CN112970373B - Carex viridis seed initiator and using method thereof - Google Patents
Carex viridis seed initiator and using method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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Abstract
The invention provides an bryophyte viridis seed initiator and a using method thereof, belonging to the technical field of seed treatment agents. KNO 3 Application of water solution or gibberellin water solution in germination of Carex viridis seeds. Also provided are initiators comprising aqueous NaOH or any ofMeans one or two agents: KNO 3 Aqueous solutions and/or aqueous gibberellin solutions. Adopting 5 to 15 percent KNO 3 120 to 480 mg/L of an aqueous solution ‑1 After the carex breviculmis seeds are initiated by the gibberellin aqueous solution, the germination vigor, the germination index and the germination rate of the seeds are improved, and the germination time is shortened; while using KNO 3 The germination of the green carex breviculmis seeds can be further promoted by the aqueous solution or/and the gibberellin aqueous solution which is initiated and combined with the 10% NaOH aqueous solution. The method has the advantages of short germination time, neat germination, high germination rate and the like, provides a good technical guarantee for direct seeding and lawn building of the carex breviculmis seeds, and is beneficial to promoting the commercial development of the carex breviculmis.
Description
Technical Field
The invention belongs to the technical field of seed germination, and particularly relates to an enteromorpha viridis seed initiator and a using method thereof.
Background
The sedge is perennial herb of sedge genus, has developed rhizome and strong ecological adaptability, early spring green turning, long green period, trampling resistance and the like. The sedge is not only an important pasture grass resource, but also an excellent lawn ground cover plant. At present, the lawn of the sedge is mainly built by means of vegetative propagation and artificial transplantation, the cost investment is very high, and the large-scale popularization and application of the sedge are greatly limited; on the other hand, under sexual propagation, the green carex breviculmis seeds are well mature, the yield is high, and a foundation is laid for directly seeding the seeds to build lawn.
With the development and progress of seed science and technology and the deep research of the biological problem of the seed, the seed initiation technology is mature day by day, it makes it stay in the second stage of imbibition through controlling the seed to absorb moisture slowly, let the seed carry on the physiological and biochemical metabolism and repair function of pregermination, in prepare to sprout and radicle not stretch out metabolic state. It can not only effectively raise the vigor of plant seed under the adverse environment and raise seed resistance, but also make its seedling emergence quick and uniform and raise seedling rate. At present, seed priming technology is mostly seen in vegetables and crops, and no report is provided for the priming research of green sedge.
Disclosure of Invention
In view of the above, the present invention aims to provide an orchis viridis seed initiator which can not only improve the germination rate and uniformity of orchis viridis, but also shorten the seed germination time.
The invention also aims to provide a using method of the initiator to promote germination of the carex breviculmis seeds, provide good technical support for direct seeding and lawn building of the carex breviculmis seeds, and facilitate promotion of commercial development of the carex breviculmis.
The invention provides KNO 3 Application of water solution or gibberellin water solution in germination of Carex viridis seeds.
Preferably, said KNO 3 The mass percentage of the aqueous solution is 5-15%.
Preferably, the concentration of the gibberellin aqueous solution comprises 120-480 mg.L -1 。
The invention provides an bryophyte viridis seed initiator, which comprises NaOH aqueous solution or any one or two of the following reagents: KNO 3 Aqueous solutions and gibberellin aqueous solutions;
the NaOH aqueous solution and KNO 3 And independently subpackaging the aqueous solution or the gibberellin aqueous solution.
Preferably, the initiator further comprises 10% by mass of an aqueous solution of NaOH;
the KNO 3 The mass percentage of the aqueous solution is 5-15%;
the concentration of the gibberellin aqueous solution comprises 120-480 mg.L -1 。
The invention provides application of the initiator in carex glaucophyllus seed germination.
The invention provides a method for promoting germination of carex glaucophyllum seeds, which comprises the following steps:
sterilizing Carex viridis seeds, and placing in KNO 3 Initiating in water solution or gibberellin water solution, cleaning, drying, and germinating.
Preferably, said KNO 3 The initiation time of the aqueous solution or the gibberellin aqueous solution is 24 hours;
the environment at the initiation is preferably a dark environment; the temperature of the environment was 25 ℃.
Preferably, the seed obtained after drying again comprises soaking the seed with 10% NaOH aqueous solution and washing again before germination;
preferably, the seed soaking time is 30-40 min.
Preferably, the seeds treated with the aqueous NaOH solution further comprise KNO 3 Reinitiating the aqueous solution or the gibberellin aqueous solution; the reinitiating solution is different from the solution used at the time of initiation;
the treatment time of the NaOH aqueous solution is 10-20 min.
KNO provided by the invention 3 Application of water solution or gibberellin water solution in germination of Carex viridis seeds. Experiments show that the proper initiator concentration can effectively solve the problems of slow and uneven germination of the carex breviculmis seeds, and 5-15% KNO is adopted 3 The aqueous solution is used as an initiator to treat the carex breviculmis seeds, compared with a control group, the germination vigor, the germination rate and the germination index of the seeds are all obviously improved, and the germination time is obviously shorter than that of the control group; when the green bryophyte seeds are treated by the gibberellin aqueous solution, the characteristics of low-concentration promotion and high-concentration inhibition are shown, so the low-concentration gibberellin solution (120-480 mg.L) -1 ) Can effectively improve the germination vigor and the germination index, accelerate the seed germination speed and shorten the germination time.
The invention provides an initiator for promoting germination of carex glaucophyllus seeds, which comprises NaOH aqueous solution and one or two of the following reagents: KNO 3 Aqueous solutions or gibberellin aqueous solutions. The experiment shows that the first pass KNO 3 Or gibberellin water solubleAfter the liquid initiation, the germination of the carex viridis seeds can be further accelerated by treating the carex viridis seeds with NaOH solutions at different times.
Meanwhile, the initiator provided by the invention also comprises KNO 3 Aqueous solutions, gibberellin aqueous solutions, and NaOH aqueous solutions. Experiments show that 5 percent KNO is firstly used 3 Initiating, soaking the seeds in 10% NaOH for 20min, and finally soaking in 480 mg. L -1 Compared with the CK (carex breviculmis seeds without any treatment), the germination potential of the seeds after gibberellin initiation treatment is improved to 92.00%, the germination index is improved to 11.23, the germination rate is improved to 98.67%, and the germination time is shortened from 9.01d to 4.55 d.
Detailed Description
The invention provides KNO 3 The solution or gibberellin water solution is applied to germination of green carex breviculmis seeds.
In the present invention, said KNO 3 The mass percentage of the aqueous solution is preferably 5% to 15%, more preferably 5% to 10%, and most preferably 5%. The invention is to the KNO 3 The method of preparing the aqueous solution is not particularly limited, and KNO known in the art is used 3 The preparation method of the aqueous solution is described. In the present invention, KNO is used alone 3 The aqueous solution initiates germination of the carex breviculmis seeds. By the KNO 3 Carex viridis seeds treated with aqueous solution, germination status and KNO thereof 3 The concentration is related, the germination vigor, the germination rate and the germination index of the seeds are increased and then decreased along with the increase of the concentration, and the average germination time is shortened and then increased. At 5% KNO 3 Under the treatment, the germination vigor, the germination rate and the germination index of the seeds are maximum values which are respectively 11.33%, 97.33% and 7.37 higher than those of a control group, the average germination time is 11.33%, 4.66% and 2.06 higher than those of the control group, the average germination time is the lowest value and is obviously shorter than that of the control group, and the seeds germinate 2.11 days earlier than those of the control group. When KNO 3 When the concentration is higher than 20%, each germination index is inferior to that of the control group. Thus, it can be seen that KNO is 5% to 15% 3 The water solution can effectively accelerate Carex viridisThe seed germination is one of effective methods for solving the problems of slow germination and uneven germination.
In the invention, the concentration of the gibberellin aqueous solution preferably comprises 120-480 mg.L -1 More preferably 240 to 480 mg.L -1 Most preferably 480 mg.L -1 . With the increase of the concentration of the gibberellin aqueous solution, the germination vigor, the germination rate and the germination index (average germination time) of the seeds increase (shorten) and then decrease (increase). The germination potential and the germination index are 480 mg.L in gibberellin aqueous solution -1 The maximum values reached are respectively 32.00% and 8.00, which are respectively improved by 32.00% and 2.69 compared with the reference; the average germination time is 240 mg.L -1 The minimum time of the gibberellin aqueous solution treatment is 6.12 days, which is 2.89 days earlier than that of the control. Researches show that the gibberellin has the characteristics of low-concentration promotion and high-concentration inhibition on the germination of the Carex viridis seeds, so that the germination vigor and the germination index of the Carex viridis seeds can be effectively improved by the aid of a low-concentration gibberellin aqueous solution, and the germination is accelerated.
The invention provides an initiator for germination of carex glaucophyllus seeds, which comprises NaOH aqueous solution or any one or two of the following reagents: KNO 3 Aqueous solutions and gibberellin aqueous solutions; the aqueous NaOH solution and KNO 3 And (4) independently subpackaging the aqueous solution or the gibberellin aqueous solution.
In the present invention, the NaOH aqueous solution preferably has a mass percentage of 10%. The KNO 3 The mass percentage of the aqueous solution is preferably 5% to 15%, more preferably 5% to 10%, and most preferably 5%. The concentration of the gibberellin aqueous solution preferably comprises 120-480 mg.L -1 More preferably 240 to 480 mg.L -1 Most preferably 480 mg.L -1 . Experiments show that the KNO is firstly used 3 When the aqueous solution or the gibberellin aqueous solution is used for initiation, and the carex breviculmis seeds are treated by the NaOH aqueous solution, the germination vigor, the germination index and the average germination time are respectively 92.00%, 11.03 and 4.53d, and the germination rate is up to 100.00% when the carex brevicis seeds are treated by the NaOH for 30min, which shows that the initiator can further accelerate the germination.
The invention provides application of the initiator in germination of carex viridis seeds.
In the present invention, the treatment method of the initiator in the germination of the carex glaucophyllus seeds preferably comprises the following steps: and (3) disinfecting and cleaning the carex viridis seeds, then placing the carex viridis seeds in the initiator for treatment, and germinating the treated seeds. The method of sterilization in the present invention is not particularly limited, and a sterilization method known in the art may be used. In the embodiment of the invention, the disinfection is finished by soaking in a 10% sodium hypochlorite solution for 20 min. The washing is preferably performed by washing the seeds with distilled water. The number of times of flushing is preferably 5-6. The treatment is preferably carried out at 25 ℃. The environment at the time of the treatment is preferably dark conditions. The time of the treatment is preferably 24 h. The treatment preferably immerses the sterilized seed in the initiating agent. After said treatment, it preferably comprises washing and draining the primed seed. The washing is preferably performed with distilled water. The drying time is preferably 48 h. The temperature of the drying is preferably 18-25 ℃, and more preferably 23 ℃.
In the present invention, when the carex viridis seed is treated with the initiator containing the aqueous NaOH solution, it is preferable to first use the KNO 3 Treating with water solution or gibberellin water solution, cleaning, drying, treating with NaOH water solution, cleaning, drying, and germinating. The time for treating the seeds by the NaOH aqueous solution is preferably 30-40 min. Experiments show that 480 mg.L is used -1 After the initiation of the gibberellin aqueous solution, with the increase of the treatment time of the NaOH aqueous solution, the germination potential, the germination rate and the germination index show the tendency of increasing firstly and then decreasing, the maximum values of the germination potential and the germination rate are respectively 80.00 percent and 98.00 percent when the gibberellin aqueous solution is treated for 30min, and the optimal value of the germination index is 10.60 when the gibberellin aqueous solution is treated for 40 min; the average germination time is shortened and increased with the increase of the treatment time, and the minimum time required for germination is 4.63d at 40min of treatment. Simultaneously, the invention firstly uses 480 mg.L -1 The gibberellin aqueous solution is used for initiating the seeds, and the seeds are soaked in the 10% NaOH aqueous solution for 30-40 min, so that a good germination effect can be achieved, the germination vigor and the germination index of the seeds are obviously improved, and the germination time of the seeds is shortened.
In the present invention, when the initiator contains KNO together 3 Aqueous solution,When the gibberellin aqueous solution and the NaOH aqueous solution are used, the method for promoting the germination of the carex breviculmis seeds by using the initiator is preferably to firstly use the KNO 3 Subjecting the sterilized seed to first initiation with water solution or gibberellin water solution, cleaning, drying, treating with NaOH water solution, cleaning, drying, and adding gibberellin water solution or KNO 3 The aqueous solution is used for secondary initiation (the solution for secondary initiation is different from the solution for primary initiation), and germination is carried out after washing and drying. Except that the first initiation and the second initiation are different in solution, the initiation time, temperature, environment and other conditions are consistent, and the detailed description is omitted. The germination indexes of the seeds are influenced by the mixed treatment of multiple initiation and different-time NaOH aqueous solution treatment on the carex breviculmis seeds, specifically, with the increase of the treatment time of the NaOH aqueous solution, the germination vigor, the germination rate and the germination index of the seeds are increased and then decreased, the average germination time is shortened and then increased, the germination vigor, the germination index and the average germination time reach the optimal values when the seeds are treated by the NaOH aqueous solution for 20min, the optimal values are respectively 92.00, 11.23 and 4.46d, the highest value of the germination rate when the seeds are treated by the NaOH aqueous solution for 10min is 98.67%, and the germination rate is not obviously different from the germination rate under 20 min. First, 5% KNO is used 3 Initiating with water solution, soaking the seeds in 10% NaOH water solution for 10-20 min, and finally soaking in 480 mg.L -1 Compared with CK (carex breviculmis seeds without any treatment), the germination vigor of the carex breviculmis initiated by the gibberellin aqueous solution is improved by 92.00 percent, the germination index is improved by 6.02 percent, the germination rate is improved by 98.67 percent, and the germination time is shortened from 9.01d to 4.46d, so that the method is the optimal treatment scheme for improving the germination index and advancing the germination time of the carex breviculmis seeds.
The invention provides a method for promoting germination of carex breviculmis seeds, which comprises the following steps:
sterilizing Carex viridis seeds, and placing in KNO 3 Initiating in water solution or gibberellin water solution, cleaning, drying, and performing germination culture on the dried seeds.
In the present invention, said KNO 3 The initiation time of the aqueous solution or gibberellin aqueous solution is preferably 24 h. The environment at the initiation is preferably a dark environment; the temperature of the environment is preferably 25 ℃.
In the present invention, the step of obtaining the redried seeds preferably further comprises soaking the seeds in a 10% NaOH aqueous solution, washing again and redrying the seeds before germination culture; the seed soaking time is 30-40 min.
In the present invention, the seed treated with the aqueous NaOH solution preferably further comprises KNO 3 Reinitiating the aqueous solution or the gibberellin aqueous solution; the reinitiating solution is different from the solution used during the initiation, i.e. the solution used for initiation before treatment with the aqueous NaOH solution is KNO 3 In the case of aqueous solutions, the solution used for reinitiating the seeds treated with aqueous NaOH solution is an aqueous gibberellin solution. The invention provides a method for using 5% KNO 3 Initiating with water solution, soaking the seeds in 10% NaOH water solution for 20min, and soaking in 480 mg. L -1 The gibberellin aqueous solution-initiated scheme achieves the best effect on the improvement of indexes such as germination vigor, germination index, germination rate and germination time.
In the present invention, the germination culture is preferably performed on filter paper, and the medium for germination is preferably distilled water. The germination period is preferably light cultivation. The light intensity in the illumination culture is preferably 23000-25000 Lx, and more preferably 24000 Lx. The photoperiod is preferably 16h light/8 h dark. The temperature for germination is preferably 25 ℃. The humidity of the germination environment is preferably 60% to 80%, more preferably 70%. The germination judgment standard is preferably based on 'white exposure', and the counting standard is that the radicle breaks through the seed coat to exceed the seed length 1/2.
The following examples are provided to illustrate the green sedge seed initiator and the method of using the same in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
Selecting full-seed Carex viridis seeds, placing into a 50ml centrifuge tube, soaking with 10% sodium hypochlorite for 20min for sterilization, shaking continuously, and washing with distilled water for 5 times after sterilization. Respectively applying 0% (CK), 5%, 10%, 15%, 20%, 25% KNO to the sterilized seeds 3 Completely immersing the solution, initiating in dark environment at 25 deg.C for 24 hr, washing with distilled water, and standing at room temperature (25 deg.C)And (5) after drying for 48h, performing a germination test.
The germination test was repeated 1 time with 50 seeds, and the seeds were placed in a germination box with 12cm × 12cm × 5cm format and 3 repeats of 3 layers of filter paper placed inside the box and cultured in distilled water. The germination box is placed in an illumination incubator with the light intensity 24000Lx, the temperature of 25 ℃ and the humidity of 60-80 percent for culture, and the germination photoperiod is 16 hours of illumination and 8 hours of darkness. Distilled water was added to the solution every day in a fixed amount to keep it moist. Taking 'white dew' as a germination standard, taking 1/2 as a counting standard for the root of the embryo breaking through seed coat exceeding seed length, counting the germination number once per day, setting the germination test period to be 30d, and calculating the germination vigor, the germination rate, the germination index and the average germination time according to formulas I-IV.
The seed germination potential is the number of the germinated seeds in the initial period (5 th day)/the number of the tested seeds multiplied by 100 percent of formula I;
the germination rate of the seeds is the number of germinated seeds in the final stage (day 15)/the number of tested seeds multiplied by 100 percent of formula II;
germination index ═ Σ (Gt/Dt) formula III;
wherein Gt is the number of the germination seeds of the current day, and Dt is the corresponding germination days.
Average germination time ∑ nt/Σ n formula IV
Wherein: n is the number of newly germinated seeds at time t, and t is the germination time.
The statistical results are shown in Table 1.
TABLE 1 different concentrations of KNO 3 Germination index of green moss seeds triggered by solution
Note: different letters indicate significant differences, p < 0.05.
As can be seen from Table 1, the germination status and KNO of the Carex viridis seeds 3 The concentration is related, the germination vigor, the germination rate and the germination index of the seeds are increased and then decreased along with the increase of the concentration, and the average germination time is shortened and then increased. At 5% KNO 3 Under the treatment of aqueous solution, the germination potential, germination rate and germination index of the seeds are the mostThe germination time is 11.33%, 4.66% and 2.06% respectively, the average germination time reaches the lowest value, is obviously shorter than that of a control group, and the germination time is advanced by 2.11 days compared with that of the control group; when KNO 3 When the concentration of the aqueous solution is higher than 20%, each germination index is inferior to that of the control group, which indicates that the concentration of KNO is low 3 The water solution (5-15%) can accelerate the germination speed of the green sedge seeds, and is one of effective methods for solving the problems of slow germination and uneven germination.
Example 2
Respectively sterilizing the seeds with 0 mg.L -1 (CK)、60mg·L -1 、120mg·L -1 、240mg·L -1 、480mg·L -1 、960mg·L -1 The gibberellin aqueous solution is completely immersed, placed in a dark environment at 25 ℃ for initiation for 24 hours, washed with distilled water after initiation, dried back at room temperature (18 ℃) for 48 hours, and then subjected to a germination test. The germination test method was the same as in example 1.
The statistical results are shown in Table 2.
TABLE 2 Germination index of green moss seeds induced by gibberellin water solutions of different concentrations
Note: the different letters represent significant differences, p < 0.05.
As can be seen from Table 2, the germination vigor, germination rate and germination index (average germination time) of the seeds increased (shortened) and then decreased (increased) with the increase in gibberellin concentration. At 120-480 mg.L -1 In the range, compared with a control group, the germination indexes of the plants are obviously different except the germination rate, and the germination vigor and the germination index are 480 mg.L -1 The maximum values reached under the concentration are 32.00 percent and 8.00 percent respectively, which are respectively improved by 32.00 percent and 2.69 compared with a control; the average germination time is 240 mg.L -1 The minimum time for gibberellin treatment was 6.12d, which is 2.89d earlier than the control group. Research shows that gibberellin has the characteristics of low-concentration promotion and high-concentration inhibition on germination of the green bryophyte seeds,therefore, the gibberellin aqueous solution with low concentration can effectively improve the germination vigor and the germination index and accelerate the germination.
Example 3
Sterilizing the seeds with 5% KNO 3 The solution is initiated for 24 hours in a dark environment at 25 ℃, washed by distilled water after initiation, dried back for 48 hours at room temperature (23 ℃), the seeds after drying back are respectively soaked in 10 percent NaOH aqueous solution for 0min (CK), 10min, 20min, 30min, 40min and 50min, and repeatedly washed by distilled water after treatment until the pH value is 7.0, and then the germination test is carried out. The germination test method was the same as in example 1.
TABLE 35% KNO 3 Germination index of seeds in 10% NaOH aqueous solution at different times after solution initiation
Note: the representation was significantly different between different letters, p < 0.05.
As can be seen from Table 3, the Carex viridis seeds were first subjected to 5% KNO 3 After the initiation of the aqueous solution, the germination can be further accelerated by treating the seeds with NaOH aqueous solutions at different times. With the increase of the treatment time, the germination vigor, the germination rate and the germination index of the seeds are increased and then reduced, and the average germination time is shortened and then increased. The germination vigor, the germination index and the average germination time all reach the optimal values when the NaOH aqueous solution is treated for 40min, and respectively reach 92.00%, 11.03 and 4.53d, and the germination rate reaches the maximum of 100.00% when the NaOH aqueous solution is treated for 30 min. Thus the seed is subjected to KNO 3 After the initiation of the aqueous solution, the seeds are soaked in NaOH aqueous solution for 30-40 min, so that various germination indexes can be effectively improved.
Example 4
Sterilizing the seeds with 480 mg.L -1 Inducing gibberellin water solution at 25 deg.C in dark for 24 hr, washing with distilled water, and drying at room temperature (20 deg.C)And soaking the dried seeds in 10% NaOH aqueous solution for 0min (CK), 10min, 20min, 30min, 40min and 50min respectively for 48h, repeatedly washing the seeds with distilled water until the pH value is 7.0 after treatment, and then performing a germination test. The germination test method was the same as in example 1.
TABLE 4480 mg. L -1 Germination index of 10% NaOH aqueous solution after initiation of gibberellin aqueous solution at different times
Note: the different letters represent significant differences, p < 0.05.
As can be seen from Table 4, the Carex viridis seeds were first applied at 480 mg.L -1 After initiation of gibberellin aqueous solution, seeds were soaked in NaOH aqueous solution for different periods of time, which also had some effect on various germination indicators (table 4). With the increase of the NaOH treatment time, the germination potential, the germination rate and the germination index show the tendency of increasing firstly and then decreasing, the maximum values of the germination potential and the germination rate are respectively 80.00 percent and 98.00 percent when the NaOH treatment time is 30min, and the optimal value of the germination index is 10.60 when the NaOH treatment time is 40 min; the average germination time is shortened and increased with the increase of the treatment time, and the minimum time required for germination is 4.63d at 40min of treatment. The research shows that the bryopteris viridis seeds are firstly used with 480 mg.L -1 The gibberellin aqueous solution is initiated and then the seeds are soaked in 10% NaOH for 30-40 min, so that a good germination effect can be achieved, the germination vigor and the germination index of the seeds are obviously improved, and the germination time of the seeds is shortened.
Example 5
Sterilizing the seeds with 5% KNO 3 The solution is initiated in dark environment at 25 deg.C for 24 hr, washed with distilled water, dried at room temperature (24 deg.C) for 48 hr, soaked in 10% NaOH solution for 0min (CK), 10min, 20min, 30min, 40min, and 50min, washed with distilled water repeatedly until pH is 7.0, and treated with 480 mg. L. seed -1 The gibberellin aqueous solution is initiated for 24 hours in a dark environment at 25 ℃, washed with distilled water after initiation, dried back for 48 hours at room temperature (24 ℃) and then subjected to a germination test.
TABLE 5 KNO 3 After the solution is initiated, 10 percent NaOH solution is used for soaking seeds for different time, and then the germination index of the seeds is initiated by gibberellin aqueous solution
Note: the different letters represent significant differences, p < 0.05.
As can be seen from table 5, the mixed treatment of multiple priming of the carex breviculmis seeds and 10% NaOH aqueous solution treatment at different times has an effect on the germination indicator of the seeds. With the increase of the treatment time of the NaOH aqueous solution, the germination potential, the germination rate and the germination index of the seeds are increased firstly and then reduced, the average germination time is shortened firstly and then increased, the germination potential, the germination index and the average germination time reach the optimal values respectively of 92.00, 11.23 and 4.46 days when the seeds are treated by the NaOH aqueous solution for 20min, the highest value of the germination rate reaches 98.67% when the seeds are treated by the NaOH aqueous solution for 10min, and the germination rate is not obviously different from the germination rate under 20 min. The results show that the treatment can obviously improve the germination condition of seeds by first using 5 percent KNO 3 Initiating with water solution, soaking seeds with 10% NaOH water solution for 20min, and finally soaking seeds with 480 mg.L -1 Compared with a CK (Carex virens seed without any treatment), the germination potential of the seed is improved to 92.00%, the germination index is improved to 11.23, the germination rate is improved to 98.67% from 92.67%, and the germination time is shortened to 4.46d from 9.01d by adopting gibberellin aqueous solution initiation, so that the method is the optimal treatment for improving the germination index and advancing the germination time of the Carex virens seed, and is the most effective scheme for solving the problems of slow germination, irregular emergence and the like of the Carex virens seed.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (3)
1. An initiator for germination of carex breviculmis seeds, which is characterized in thatThe initiator is NaOH aqueous solution or KNO 3 Aqueous solution and gibberellin aqueous solution;
the aqueous NaOH solution and KNO 3 Independently packaging the aqueous solution or gibberellin aqueous solution;
the concentration of the gibberellin aqueous solution is 480 mg.L -1 ；
The KNO 3 The mass percentage of the aqueous solution is 5 percent;
the mass percentage of the NaOH aqueous solution is 10%.
2. Use of the initiator of claim 1 for germination of green carex grass seeds.
3. A method for promoting germination of carex breviculmis seeds is characterized by comprising the following steps:
sterilizing Carex viridis seeds, placing the sterilized Carex viridis seeds into gibberellin water solution for initiation, cleaning, drying, and germinating the dried seeds;
soaking the seeds in 10 percent NaOH aqueous solution by mass percentage and cleaning again before the seeds are germinated; the seeds treated by the NaOH aqueous solution also comprise KNO with the mass percentage of 5 percent 3 Reinitiating the aqueous solution; the time for treating the NaOH aqueous solution is 10-20 min;
the concentration of the gibberellin aqueous solution is 480 mg.L -1 ；
The initiation time of the gibberellin aqueous solution is 24 h;
the KNO 3 The initiation time of the aqueous solution is 24 h;
the environment at the initiation is a dark environment; the temperature of the environment was 25 ℃.
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