CN111010920B - Method for breaking dormancy of gravelly sedum graveolens seeds - Google Patents
Method for breaking dormancy of gravelly sedum graveolens seeds Download PDFInfo
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Abstract
The invention discloses a method for breaking dormancy of gravelly sedum aizoon seeds, which comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment; soaking in the gibberellin solution: taking out the sinking seeds, sucking the surface water, placing the sinking seeds in a beaker, adding 400mL of gibberellin solution, soaking for 20-24 h, stirring once every 1.5-2 h during soaking, and washing the soaked seeds with clear water for later use after soaking; sand storage treatment of the seeds: controlling the sand storage temperature at minus 7 ℃ to minus 3 ℃, stirring and turning over the sand storage seeds every 5-8 days, keeping good ventilation, taking out the seeds at 45-65 days, performing running water spraying treatment for 10-14 h, and airing for later use. After the Carex chaenomeles seeds are treated by gibberellin, the permeability of seed coat solute is increased, and after the Carex chaenomeles seeds are fully absorbed by sand storage, the germination rate of the seeds is improved, so that the original germination rate of 7 percent is improved to 65-75 percent. The germination time is shortened from 22 days to 10-15 days, and the seed dormancy is effectively broken.
Description
Technical Field
The invention relates to the technical field of plant germination and breeding, in particular to a method for breaking the dormancy of gravelly sedum graveolens seeds.
Background
The Meadowrue latching grass is a wild cultivated species which is formed by artificially domesticating and breeding the local wild Meadowrue latching grass in inner Mongolia, the leaf color is green, and the texture is fine and soft; early green turning, strong drought resistance and cold resistance, almost no serious plant diseases and insect pests, slow growth speed and manageable extensive use. Can be used for urban and rural environmental greening and ecological environment construction, and is a high-quality lawn grass seed. However, the hordei horridioides has weak sexual reproduction capability, low seed yield and the phenomenon of seed dormancy. Therefore, the lawn can not be established by using the direct seeding mode of the earth humulus graveolens seeds without solving the problem of seed germination. At present, the study on the dormancy and germination characteristics of the carex breve seeds is less, and the report on breaking the carex breve seed dormancy at home and abroad is less.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides a method for breaking the dormancy of the gravelly sedum graveolens seeds, which can greatly shorten the germination time of the gravelly sedum graveolens seeds and improve the germination rate of the seeds.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the Carex chaenomeles seeds are collected and air-dried, weighing 10-20 g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 1-2 hours, and taking out the settled seeds after 20-24 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, placing the sinking seeds in a beaker, adding 400mL of gibberellin solution, soaking for 20-24 h, stirring once every 1.5-2 h during soaking, and washing the soaked seeds clean with clear water for later use after soaking;
(3) sand storage treatment of the seeds: and (3) storing the seeds treated in the step (2) in sand at a low temperature, controlling the sand storage temperature to be within-7 ℃ to-3 ℃, stirring and turning the sand-stored seeds every 5-8 days, keeping good ventilation, taking out the seeds at 45-65 days, performing flowing water spraying treatment for 10-14 hours, and airing for later use.
Moreover, the method for breaking the dormancy of the gravelly sedum graveolens seeds comprises the three steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, weighing 15g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 2 hours, and taking out the settled seeds after 24 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 24h, stirring once every 2h during soaking, and washing the soaked seeds with clean water for later use after soaking;
(3) sand storage treatment of the seeds: and (3) storing the seeds treated in the step (2) in sand at a low temperature, controlling the sand storage temperature to be-5 ℃, stirring and turning the sand stored seeds every 7 days, keeping good ventilation, taking out the seeds at 50 days, performing running water showering treatment for 12 hours, and airing for later use.
Moreover, the concentration of the gibberellin solution is 350 mg/L-550 mg/L.
Moreover, the seed sand storage treatment: the ratio of the seeds to the sand is 1: 3-1: 4 when the sand is stored in the sand, and the water content of the sand is 20-30%.
The invention has the advantages and positive effects that:
1. after the Carex chaenomeles seeds are treated by gibberellin, the permeability of seed coat solute is increased, and after the Carex chaenomeles seeds are fully absorbed by sand storage, the germination rate of the seeds is improved, so that the original germination rate of 7 percent is improved to 65-75 percent. The germination time is shortened from 22 days to 10-12 days, and the seed dormancy is effectively broken.
2. The seeds treated by the method are applied in the actual sowing process, so that a good effect is achieved, and the germination rate after sowing can reach 65-75% in the actual production and use process. The germination time is shortened from the original 22 days to 15 days, and the application prospect is wide.
Drawings
Fig. 1 is a schematic view of lawn growth.
Detailed Description
For a further understanding of the contents, features and effects of the present invention, the following embodiments are exemplified and explained in detail with reference to the accompanying drawings. It should be noted that the present embodiment is illustrative, not restrictive, and the scope of the invention should not be limited thereby.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available unless otherwise specified.
For convenience of description and application, the unit of the seeds in the claims is expressed in g, the unit of the solution is expressed in mL, and the dosage of the seeds is proportionally increased or decreased in the practical application process, which belongs to the protection scope of the invention.
The method for evaluating the germination rate of the gravelly sedum aizoon seeds in a laboratory comprises the following steps: the treated seeds were germinated in a climatic chamber, the treated bryophyte seeds were placed in petri dishes with 12cm diameter, 10 mL of distilled water was added to each dish. Placing the culture dish into an artificial climate incubator, keeping the germination temperature at 10 ℃, keeping the filter paper wet all the time during the germination process, counting the germination number every day, and calculating the germination rate after the germination is finished.
Germination rate: the filter paper is kept wet in the test process, the length of the radicle protruding seed coat exceeds the length of the seed is taken as the germination standard, and the germination rate = the number of the germinated seeds/the number of the tested seeds multiplied by 100%.
Example 1
A method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, weighing 10g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 1 hour, and taking out the settled seeds after 20 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 20h, stirring once every 1.5h during soaking, and washing the soaked seeds with clear water for later use after soaking;
(3) sand storage treatment of the seeds: sand-storing the seeds treated in the step (2) at a low temperature, controlling the sand-storing temperature to be-7 ℃, stirring and turning the sand-stored seeds every 5 days, keeping good ventilation, taking out the seeds at 45 days, performing running water showering treatment for 10 hours, and airing for later use;
the concentration of the gibberellin solution is 350 mg/L; sand storage treatment of the seeds: the ratio of the seeds to the sand is 1:3 when the sand is stored in the sand, and the water content of the sand is 20 percent.
Example 2
A method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, weighing 15g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 2 hours, and taking out the settled seeds after 24 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 24h, stirring once every 2h during soaking, and washing the soaked seeds with clean water for later use after soaking;
(3) sand storage treatment of the seeds: sand-storing the seeds treated in the step (2) at a low temperature, controlling the sand-storing temperature to be-5 ℃, stirring and turning the sand-stored seeds every 7 days, keeping good ventilation, taking out the seeds at 50 days, performing running water showering treatment for 12 hours, and airing for later use;
the concentration of the gibberellin solution is 400 mg/L; sand storage treatment of the seeds: the ratio of the seeds to the sand is 1:4 when the sand is stored, and the water content of the sand is 22%.
Example 3
A method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophorina dranthema are collected and air-dried, weighing 20g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 1.5 hours, and taking out the settled seeds after 22 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 22h, stirring once every 1.8h during soaking, and washing the soaked seeds with clear water for later use after soaking;
(3) sand storage treatment of the seeds: sand-storing the seeds treated in the step (2) at a low temperature, controlling the sand-storing temperature to be-3 ℃, stirring and turning the sand-stored seeds every 8 days, keeping good ventilation, taking out the seeds at 65 days, performing running water flushing treatment for 14 hours, and airing for later use;
the concentration of the gibberellin solution is 450 mg/L; sand storage treatment of the seeds: the ratio of the seeds to the sand is 1:3 when the sand is stored in the sand, and the water content of the sand is 25 percent.
Example 4
A method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, 18g of seeds are weighed and soaked in a beaker filled with 400mL of distilled water, the beaker is placed in a constant-temperature incubator at the temperature of 25 ℃, the mixture is stirred for 1 time every 1.5 hours, and the sunk seeds are taken out after 21 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 21h, stirring once every 1.7h during soaking, and washing the soaked seeds with clear water for later use after soaking;
(3) sand storage treatment of the seeds: and (3) storing the seeds treated in the step (2) in sand at a low temperature, controlling the sand storage temperature to be-6 ℃, stirring and turning the sand stored seeds every 6 days, keeping good ventilation, taking out the seeds at 60 days, performing running water showering treatment for 12 hours, and airing for later use.
The concentration of the gibberellin solution is 500 mg/L; sand storage treatment of the seeds: the ratio of the seeds to the sand is 1:4 when the sand is stored, and the water content of the sand is 28 percent.
Example 5
A method for breaking the dormancy of the seeds of the Geranium graveolens comprises the steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, weighing 12g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 2 hours, and taking out the settled seeds after 23 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 23h, stirring once every 1.6h during soaking, and washing the soaked seeds with clear water for later use after soaking;
(3) sand storage treatment of the seeds: sand-storing the seeds treated in the step (2) at a low temperature, controlling the sand-storing temperature to be-4 ℃, stirring and turning the sand-stored seeds every 7 days, keeping good ventilation, taking out the seeds at 55 days, performing running water showering treatment for 12 hours, and airing for later use;
the concentration of the gibberellin solution is 600 mg/L; sand storage treatment of the seeds: the ratio of the seeds to the sand is 1:3.5 when the sand is stored in the sand, and the water content of the sand is 30 percent.
The seeds treated in examples 1 to 5 were germinated in a climatic chamber, and the treated carex breve seeds were placed in petri dishes with a double layer of filter paper, each dish having a diameter of 12cm, and 10 mL of distilled water was added. Placing the culture dish into an artificial climate incubator, keeping the germination temperature at 10 ℃, keeping the filter paper wet all the time during the germination process, counting the germination number every day, and calculating the germination rate after the germination is finished.
The results of the germination percentage tests of examples 1 to 5 are shown in Table 1:
germination rate of seeds
Gibberellin concentration (mg/L) | Percentage of germination (%) |
Example 1 | 69 |
Example 2 | 75 |
Example 3 | 72 |
Example 4 | 73 |
Example 5 | 65 |
Experiment 1: effect of gibberellin concentration on germination Rate
In the experiment A group, gibberellin concentrations are respectively selected from 0, 200, 350, 400, 550, 600, 800 and 1000mg/L for treatment, sand storage treatment is not carried out on seeds, other treatment steps are the same as those in the example 2, and a germination experiment is carried out;
the gibberellin concentrations in the group B of the experiment are respectively selected from 0, 200, 350, 400, 550, 600, 800 and 1000mg/L for treatment, and other treatment steps are the same as those in the embodiment 2 to carry out a germination experiment;
the results of the seed germination experiments are shown in table 2, and it can be seen from table 2 that:
(1) the gibberellin has the characteristics of low-concentration promotion and high-concentration inhibition on germination of the Carex meyeriana seeds, the concentration of the gibberellin is preferably 350-550 mg/L, and meanwhile, as can be seen from the table 2, even if the concentration of the gibberellin is the same, the germination rate of the seeds cannot meet the use requirement due to the lack of a seed sand storage treatment step; the germination rate of the seeds subjected to sand storage treatment can be more than 2 times, so that the importance of the sand storage treatment step of the seeds can be seen;
(2) when the concentration (mg/L) of gibberellin is 0, the germination rates of the group A and the group B are close, which indicates that the germination rate of the seeds is still very low without being soaked by gibberellin with a certain concentration, and indicates that the seeds can play a role of breaking the seeds of the Geranium brevifilifolium only after being soaked by the gibberellin with a certain concentration in the sand storage treatment of the seeds, and the seeds are not suitable for all.
TABLE 2 germination percentage of seeds treated with gibberellins of various concentrations
Gibberellin concentration (mg/L) | Group A germination (%) | Group B germination (%) |
0 | 7 | 8 |
200 | 21 | 40 |
350 | 30 | 69 |
400 | 34 | 75 |
550 | 32 | 72 |
600 | 28 | 68 |
800 | 6 | 47 |
1000 | 0 | 0 |
Experiment 2 influence of the number of days of sand storage treatment on the germination time
Respectively placing the seeds in a refrigeration house at the temperature of-5 ℃ for 0d, 30d, 45d, 60d and 90d, stirring and turning the seeds every 7d, keeping good ventilation, and the other steps are the same as the step in the embodiment 2; germination test seeds are placed in a culture dish and placed in an artificial climate incubator for germination test, wherein the germination temperature is 10 ℃.
The results of the experiment are shown in table 3: when the number of days for sand storage treatment of the seeds is 45-60 days, the germination time of the seeds is obviously shortened, the germination rate is obviously improved, the germination rate of the seeds is not obviously increased after the number of days for sand storage treatment is 60 days, and the number of days for sand storage treatment is preferably 45-60 days.
TABLE 3 influence of the days of sand storage treatment on the germination percentage and germination time of seeds
Days of sand storage (d) | Germination rate(%) | Minimum time for Germination (d) |
0 | 34 | 22 |
30 | 45 | 12 |
45 | 65 | 11 |
60 | 75 | 11 |
90 | 78 | 10 |
Experiment 3 actual sowing experiment
Selecting the seeds treated in the examples 1-5, carrying out actual production application, and directly planting the lawn in a seed direct seeding mode;
experiments are carried out in 2019 at the bottom of 8 months in a way of calling the left flag of the Alternanther sieboldii, after the seeds are sowed, daily care is carried out, the seeds begin to germinate 12-15 days after being sowed, the germination rate of the seeds of the Erythrochloe breve is 65-75%, and the lawn basically grows 45-60 days (as shown in figure 1).
Claims (2)
1. A method for breaking the dormancy of the seeds of the gravelly sedum graveolens comprises three steps of seed pretreatment, gibberellin solution soaking and seed sand storage treatment, and is characterized in that:
(1) the seed pretreatment: after the Carex chaenomeles seeds are collected and air-dried, weighing 10-20 g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 1-2 hours, and taking out the settled seeds after 20-24 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, placing the sinking seeds in a beaker, adding 400mL of gibberellin solution, soaking for 20-24 h, stirring once every 1.5-2 h during soaking, and washing the soaked seeds clean with clear water for later use after soaking;
(3) sand storage treatment of the seeds: storing the seeds treated in the step (2) in sand at a low temperature, controlling the sand storage temperature to be-7 ℃ to-3 ℃, stirring and turning the sand-stored seeds every 5-8 days, keeping good ventilation, taking out the seeds at 45-65 days, performing running water spraying treatment for 10-14 hours, and airing for later use;
the concentration of the gibberellin solution is 350 mg/L-550 mg/L;
sand storage treatment of the seeds: the ratio of the seeds to the sand is 1: 3-1: 4 when the sand is stored in the sand, and the water content of the sand is 20-30%.
2. A method of breaking dormancy of gravelly sedum seeds according to claim 1, wherein:
(1) the seed pretreatment: after the seeds of the bryophora terezans are harvested and air-dried, weighing 15g of seeds, soaking the seeds in a beaker filled with 400mL of distilled water, placing the beaker in a constant-temperature incubator at 25 ℃, stirring for 1 time every 2 hours, and taking out the settled seeds after 24 hours;
(2) soaking in the gibberellin solution: sucking the surface water of the sinking seeds taken out in the step (1) to be dry, putting the sinking seeds into a beaker, adding 400mL of gibberellin solution, soaking for 24h, stirring once every 2h during soaking, and washing the soaked seeds with clean water for later use after soaking;
(3) sand storage treatment of the seeds: and (3) storing the seeds treated in the step (2) in sand at a low temperature, controlling the sand storage temperature to be-5 ℃, stirring and turning the sand stored seeds every 7 days, keeping good ventilation, taking out the seeds at 50 days, performing running water showering treatment for 12 hours, and airing for later use.
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