CN112931700A - Composition containing deep sea fish collagen peptide and preparation method and application thereof - Google Patents
Composition containing deep sea fish collagen peptide and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
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- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to the technical field of collagen application, and particularly relates to a composition containing deep sea fish collagen peptide, and a preparation method and application thereof. The composition provided by the invention mainly comprises the following components in parts by weight: 3-5 parts of deep sea fish collagen peptide, 0.5-1.0 part of vitamin D, 0.3-0.5 part of vitamin E, 0.1-0.3 part of citric acid, 0.4-0.8 part of sodium bicarbonate, 1.0-1.5 parts of liquorice, 0.8-1.5 parts of platycodon grandiflorum, 1.0-2.0 parts of compound protein powder, 0.3-0.6 part of antibacterial mixture, 0.5-0.8 part of antioxidant, 45-50 parts of corn flour, 15-18 parts of bran, 20-25 parts of soybean meal and 1.0-2.1 parts of yeast powder. The composition provided by the invention can be added into feed as a feed additive, provides various nutrients for livestock and poultry, improves the immunity of animals, and is beneficial to the healthy growth of the animals.
Description
Technical Field
The invention belongs to the technical field of collagen application, and particularly relates to a composition containing deep sea fish collagen peptide, and a preparation method and application thereof.
Background
As is well known, various nutritional ingredients required by animals should be kept in a certain proportion in feed, different animals and the same animal have different requirements on the proportion in different growth periods, and any single raw material cannot independently meet the nutritional ingredients required by the animals, so that in the practical application process, people usually mix a plurality of feed raw materials according to a proper proportion to meet the growth requirements of the animals, but in the mixing process, various problems of bacterial pollution of the feed, diarrhea of the animals, reduction of immunity and the like often occur.
Antimicrobial peptides (AMPs) are the most recently produced immunologically active molecules of organisms during long-term evolution to adapt to the environment and to find survival, are important mediators of natural immunity, play an extremely important role in host immune defense against pathogenic invasion, and are vividly called "natural antimicrobials". As a substitute of antibiotics, the development and application of antibacterial peptides are hot spots in the research of global antibacterial agents, adjuvants and immunopotentiators at present, and attract countless researchers to be constantly dedicated to the research of antibacterial peptides.
Disclosure of Invention
Aiming at the defects generally existing in the prior art, the invention provides a composition containing deep sea fish collagen peptide and a preparation method and application thereof. The composition provided by the invention can be added into feed as a feed additive, provides various nutrients for livestock and poultry, improves the immunity of animals, and is beneficial to the healthy growth of the animals.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composition containing deep sea fish collagen peptide comprises the following components in parts by weight: 3-5 parts of deep sea fish collagen peptide, 0.5-1.0 part of vitamin D, 0.3-0.5 part of vitamin E, 0.1-0.3 part of citric acid, 0.4-0.8 part of sodium bicarbonate, 1.0-1.5 parts of liquorice, 0.8-1.5 parts of platycodon grandiflorum, 1.0-2.0 parts of compound protein powder, 0.3-0.6 part of antibacterial mixture, 0.5-0.8 part of antioxidant, 45-50 parts of corn flour, 15-18 parts of bran, 20-25 parts of soybean meal and 1.0-2.1 parts of yeast powder.
Preferably, the composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder.
Preferably, the composite protein powder is prepared from methionine, valine and arginine according to a weight ratio of 10-12: 1-3: 2-5; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 8-10: 1-3; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 5-7: 2-4.
Preferably, the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture consists of allicin and reed rhizome powder in a weight ratio of 9-2; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The invention also provides a preparation method of the composition, which comprises the following steps:
s1, mixing the corn flour, the bran and the bean pulp according to the weight part at 35-40 ℃ to prepare a mixture I;
s2, keeping the temperature unchanged, and sequentially adding the vitamin D, the vitamin E, the citric acid, the sodium bicarbonate, the liquorice and the platycodon grandiflorum in parts by weight into the mixture I prepared in the step S1 to prepare a mixture II;
s3, mixing and stirring the compound protein powder, the bacteriostatic mixture and the antioxidant uniformly at 30-32 ℃ to prepare a mixture, cooling the mixture II obtained in the step S2 to 30-32 ℃, and adding the mixture into the mixture II to prepare a mixture III;
s4, cooling the mixture III obtained in the step S3 to 23-25 ℃, adding deep sea fish collagen peptide and yeast powder into the mixture, and mixing and stirring the mixture uniformly to obtain the fish collagen peptide-yeast powder.
Preferably, the mixing and stirring conditions in the step S3 are stirring for 15-20 min at a rotating speed of 80-100 rpm; the mixing and stirring condition of the step S4 is to stir for 30-60 min at a rotation speed of 150-200 rpm.
The invention also provides application of the composition as a feed additive in livestock and poultry feed.
Preferably, the addition amount of the composition as a feed additive is 5.0-8.0%.
The raw material of the deep sea fish collagen peptide selected by the invention is taken from the skin of deep sea cod, so that the safety and the naturalness of the protein are ensured; the processing process adopts international advanced multiple biological enzymes combined with a directional enzymolysis technology to successfully degrade the polypeptide with large molecular weight into collagen peptide with molecular weight of 2000-3000 daltons, and simultaneously effectively maintains the activity of the protein peptide.
In the invention, when the composition is prepared, the deep-sea fish collagen peptide is added and can be used as a feed additive, the digestion and absorption functions of animals are effectively improved, the weight of the animals can be promoted to be increased, the mixed protein powder and the vitamins in the composition provide nutrient substances for the animals, and in the further test process, the inventor also finds that the citric acid and the sodium bicarbonate are mixed for use, the feed intake of the animals can be improved, the feed waste is avoided, and meanwhile, the yeast powder and the deep-sea fish collagen peptide are added according to a specific proportion, the digestion functions of the animals can be further improved, the feed conversion ratio is reduced, and the feed utilization rate is improved; and the addition of the antibacterial composition effectively reduces the infection rate of bacteria in the process of feeding the feed by animals and reduces the risk of the animals suffering from diseases.
Compared with the prior art, the composition provided by the invention has the following advantages:
(1) according to the composition provided by the invention, the antibacterial composition is added, so that the risk of bacterial infection is effectively reduced, the animal morbidity is reduced, and the healthy growth of animals is facilitated;
(2) according to the composition provided by the invention, the deep sea fish collagen peptide is applied to the feed additive for the first time, so that the digestion and absorption functions of animals are effectively improved, and the weight increment of the animals is promoted;
(3) the composition provided by the invention can provide various nutrient substances for animals, and simultaneously can improve the feed intake of the animals, improve the feed utilization rate and avoid feed waste.
Detailed Description
The present invention is further explained with reference to the following specific examples, but it should be noted that the following examples are only illustrative of the present invention and should not be construed as limiting the present invention, and all technical solutions similar or equivalent to the present invention are within the scope of the present invention. The method and the device are operated according to the conventional technical method and the content of the instrument instruction, wherein the specific technology or condition is not indicated in the embodiment; the reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
The deep sea fish collagen peptide can be purchased from Anhui Zhonghong bioengineering limited company; the rosemary extract is obtained from Asahi Biotech limited, Anhui; the astaxanthin may be obtained from Shandong Asia Henry Biotech, Inc.; the allicin is available from Hubei Xingyingsilver river chemical Co., Ltd; the reed root powder can be purchased from Wuhanyuan City co-creation science and technology Limited.
Example 1A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 3 parts of deep sea fish collagen peptide, 0.5 part of vitamin D, 0.3 part of vitamin E, 0.1 part of citric acid, 0.4 part of sodium bicarbonate, 1.0 part of liquorice, 0.8 part of platycodon grandiflorum, 1.0 part of compound protein powder, 0.3 part of antibacterial mixture, 0.5 part of antioxidant, 45 parts of corn flour, 15 parts of bran, 20 parts of soybean meal and 1.0 part of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 10: 1: 2, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 8: 1, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 5: 2.
The preparation method of the composition comprises the following steps:
s1, mixing corn flour, bran and bean pulp in parts by weight at 35 ℃ to prepare a mixture I;
s2, keeping the temperature unchanged, and sequentially adding the vitamin D, the vitamin E, the citric acid, the sodium bicarbonate, the liquorice and the platycodon grandiflorum in parts by weight into the mixture I prepared in the step S1 to prepare a mixture II;
s3, stirring the compound protein powder, the bacteriostatic mixture and the antioxidant at the rotating speed of 80rpm for 15min at the temperature of 30 ℃ until the mixture is uniformly mixed to prepare a mixture, cooling the mixture II obtained in the step S2 to 30 ℃, and then adding the mixture into the mixture II to prepare a mixture III;
s4, cooling the mixture III obtained in the step S3 to 23 ℃, adding deep sea fish collagen peptide and yeast powder into the mixture, stirring the mixture for 30min at the rotating speed of 150rpm, and uniformly mixing the mixture to obtain the fish collagen peptide.
Example 2A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 5 parts of deep sea fish collagen peptide, 1.0 part of vitamin D, 0.5 part of vitamin E, 0.3 part of citric acid, 0.8 part of sodium bicarbonate, 1.5 parts of liquorice, 1.5 parts of platycodon grandiflorum, 2.0 parts of compound protein powder, 0.6 part of antibacterial mixture, 0.8 part of antioxidant, 50 parts of corn flour, 18 parts of bran, 25 parts of bean pulp and 2.1 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 12: 3: 5, preparing a composition; the antibacterial mixture is prepared from allicin and reed root powder in a weight ratio of 10: 3, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 7: 4.
The preparation method of the composition comprises the following steps:
s1, mixing the corn flour, the bran and the bean pulp according to the weight part at 40 ℃ to prepare a mixture I;
s2, keeping the temperature unchanged, and sequentially adding the vitamin D, the vitamin E, the citric acid, the sodium bicarbonate, the liquorice and the platycodon grandiflorum in parts by weight into the mixture I prepared in the step S1 to prepare a mixture II;
s3, stirring the compound protein powder, the bacteriostatic mixture and the antioxidant at the rotating speed of 100rpm for 20min at the temperature of 32 ℃ until the mixture is uniformly mixed to prepare a mixture, cooling the mixture II obtained in the step S2 to 32 ℃, and then adding the mixture into the mixture II to prepare a mixture III;
s4, cooling the mixture III obtained in the step S3 to 25 ℃, adding the deep sea fish collagen peptide and the yeast powder into the mixture, and stirring the mixture for 60min at the rotating speed of 200rpm until the mixture is uniformly mixed.
Example 3A composition containing deep sea fish collagen peptide
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The preparation method of the composition comprises the following steps:
s1, mixing the corn flour, the bran and the bean pulp according to the weight part at 37 ℃ to prepare a mixture I;
s2, keeping the temperature unchanged, and sequentially adding the vitamin D, the vitamin E, the citric acid, the sodium bicarbonate, the liquorice and the platycodon grandiflorum in parts by weight into the mixture I prepared in the step S1 to prepare a mixture II;
s3, stirring the compound protein powder, the bacteriostatic mixture and the antioxidant at the rotation speed of 90rpm for 18min at 31 ℃ until the mixture is uniformly stirred to prepare a mixture, cooling the mixture II obtained in the step S2 to 31 ℃, and adding the mixture into the mixture II to prepare a mixture III;
s4, cooling the mixture III obtained in the step S3 to 25 ℃, adding the deep sea fish collagen peptide and the yeast powder into the mixture, and stirring the mixture for 45min at the rotating speed of 180rpm until the mixture is uniformly mixed.
Comparative example 1A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep-sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of an antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the examples is that comparative example 1 does not contain citric acid.
Comparative example 2A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the protein powder is methionine; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the example is that the protein powder in comparative example 1 is methionine.
Comparative example 3A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from valine and arginine according to a weight ratio of 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the embodiment is that the composite protein powder in the comparative example 3 is prepared from valine and arginine according to the weight ratio of 2: 4.
Comparative example 4A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine and arginine according to a weight ratio of 11: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the embodiment is that the composite protein powder in the comparative example 4 is prepared from methionine and arginine according to the weight ratio of 11: 4.
Comparative example 5A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 1: 1: 1, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the example is that the composite protein powder in the comparative example 5 is prepared by mixing methionine, valine and arginine according to the weight ratio of 1: 1: 1.
Comparative example 6A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 1: 1, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the embodiment is that the bacteriostatic mixture in the comparative example 6 is prepared from allicin and reed rhizome powder in a weight ratio of 1: 1.
Comparative example 7A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of bacteriostatic agent, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the bacteriostatic agent is allicin; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the examples is that the bacteriostatic agent in comparative example 7 is allicin.
Comparative example 8A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 1: 1.
The composition was prepared analogously to example 3;
the difference from the examples is that the antioxidant in comparative example 8 is prepared from rosemary extract, astaxanthin in a weight ratio of 1: 1.
Comparative example 9A composition containing collagen peptide of deep sea fish
The composition comprises the following components in parts by weight: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran and 22 parts of bean pulp; the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
The composition was prepared analogously to example 3;
the difference from the example is that comparative example 9 does not contain yeast powder.
Test example 1 bacteriostatic effect test
1. Test samples: compositions prepared in examples 1 to 3 and comparative examples 6 to 7;
2. test strains: staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa;
3. the test method comprises the following steps: diluting each composition in the test sample with normal saline to 1g/mL solution, inoculating the three strains into beef soup liquid culture medium, culturing at 3 deg.C for 18 hr, and diluting the culture with normal saline to 1 × 105cfu/mL for use. And then placing 30 small test tubes on a test tube rack, wherein 5 small test tubes are arranged in each group and numbered 1-5 respectively, adding 1mL of beef soup liquid culture medium into each small test tube by using a pipette, adding 1-4 test tubes in each group by using a pipette, adding the same amount of physiological saline into 5 test tubes by using a pipette, uniformly mixing, finally adding 1mL of diluted bacterial liquid into each test tube in each group, adding all groups, placing the groups into a 3 ℃ incubator for culturing for 2h, taking out the groups, taking a ring out of each test tube by using an inoculating ring, inoculating the ring into a flat plate, placing the tubes into the 3 ℃ incubator for culturing for 2h, and taking out an observation result, wherein the mark of bacterial growth is "+" and the mark of no bacterial growth is "-".
4. And (3) test results: see table 1 for specific test results.
TABLE 1 comparison of the bacteriostatic effects of different test samples
As can be seen from Table 2, compared with the test tube negative control group No. 5, the test tubes added with the compositions of the groups 1 to 3 of the invention have no bacterial growth, which indicates that the compositions provided by the invention have bacteriostatic effect, while the comparative examples 6 to 7 have significantly reduced bacteriostatic effect due to the change of the composition of the bacteriostatic agent.
Test example 2 efficacy test
1. Test samples: compositions obtained in examples 1 to 3 and comparative examples 1 to 9;
2. test subjects: taking 130 growing-finishing pigs with 140 days old and 65 +/-3 kg of body weight, averagely dividing the growing-finishing pigs into 13 groups, 10 pigs in each group, and requiring that: all fattening pigs are healthy and have no obvious adverse characteristics;
3. the test method comprises the following steps: the compositions prepared in groups 1 to 3 of examples and comparative groups 1 to 9 of the present invention were added as feed additives to basal diets (see NRC (2012) for pig nutrition in the united states), wherein the feed additives were added in the following amounts: the addition amount of the group of example 1 is 5%, the addition amount of the group of example 2 is 8%, and the addition amounts of the group of example 3 and the groups of comparative examples 1 to 9 are 6.5%; the group containing only basal diet served as a blank control group.
Feeding the test samples to each group of test subjects respectively, updating feed every day, freely drinking water every day for 10 days continuously, measuring weight and feed intake 2 times every day, calculating initial weight, final weight, average daily gain, average daily feed intake and feed conversion ratio of each group of pigs at the end of the test, and taking the average value of 10 animals in each group for statistics. And (3) test results: specific test results are shown in table 2.
TABLE 2 test results corresponding to different test samples
As can be seen from table 2, when the composition prepared in examples 1 to 3 of the present invention is added as a feed additive to a basic ration, the feed conversion ratio is below 3.3, the feed utilization ratio is significantly increased compared to the blank control group, and the feed utilization ratio of the control group is also significantly decreased, especially in comparative example 1 and comparative example 9, the yeast powder or citric acid is removed, the synergistic effect between the components is destroyed, and the feed utilization ratio is significantly decreased.
Finally, it should be noted that the above-described embodiments are described to facilitate understanding and use of the invention by those of ordinary skill in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.
Claims (8)
1. The composition containing the deep sea fish collagen peptide is characterized by comprising the following components in parts by weight: 3-5 parts of deep sea fish collagen peptide, 0.5-1.0 part of vitamin D, 0.3-0.5 part of vitamin E, 0.1-0.3 part of citric acid, 0.4-0.8 part of sodium bicarbonate, 1.0-1.5 parts of liquorice, 0.8-1.5 parts of platycodon grandiflorum, 1.0-2.0 parts of compound protein powder, 0.3-0.6 part of antibacterial mixture, 0.5-0.8 part of antioxidant, 45-50 parts of corn flour, 15-18 parts of bran, 20-25 parts of soybean meal and 1.0-2.1 parts of yeast powder.
2. The composition of claim 1, comprising the following components and parts by weight thereof: 4 parts of deep sea fish collagen peptide, 0.8 part of vitamin D, 0.4 part of vitamin E, 0.2 part of citric acid, 0.6 part of sodium bicarbonate, 1.2 parts of liquorice, 1.2 parts of platycodon grandiflorum, 1.5 parts of compound protein powder, 0.4 part of antibacterial mixture, 0.7 part of antioxidant, 48 parts of corn flour, 16 parts of bran, 22 parts of bean pulp and 1.6 parts of yeast powder.
3. The composition as claimed in claim 1 or 2, wherein the composite protein powder is prepared from methionine, valine and arginine according to a weight ratio of 10-12: 1-3: 2-5; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 8-10: 1-3; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 5-7: 2-4.
4. The composition as claimed in claim 3, wherein the compound protein powder is prepared from methionine, valine and arginine according to a weight ratio of 11: 2: 4, preparing a composition; the antibacterial mixture is prepared from allicin and reed rhizome powder in a weight ratio of 9: 2, preparing a composition; the antioxidant is prepared from rosemary extract and astaxanthin according to a weight ratio of 6: 3, and (3).
5. A process for the preparation of a composition according to any one of claims 1 to 4, comprising the steps of:
s1, mixing the corn flour, the bran and the bean pulp according to the weight part at 35-40 ℃ to prepare a mixture I;
s2, keeping the temperature unchanged, and sequentially adding the vitamin D, the vitamin E, the citric acid, the sodium bicarbonate, the liquorice and the platycodon grandiflorum in parts by weight into the mixture I prepared in the step S1 to prepare a mixture II;
s3, mixing and stirring the compound protein powder, the bacteriostatic mixture and the antioxidant uniformly at 30-32 ℃ to prepare a mixture, cooling the mixture II obtained in the step S2 to 30-32 ℃, and adding the mixture into the mixture II to prepare a mixture III;
s4, cooling the mixture III obtained in the step S3 to 23-25 ℃, adding deep sea fish collagen peptide and yeast powder into the mixture, and mixing and stirring the mixture uniformly to obtain the fish collagen peptide-yeast powder.
6. The method according to claim 5, wherein the mixing and stirring conditions in step S3 are stirring at 80-100 rpm for 15-20 min; the mixing and stirring condition of the step S4 is to stir for 30-60 min at a rotation speed of 150-200 rpm.
7. Use of a composition according to claim 1 as a feed additive in a feed for livestock and poultry.
8. The use according to claim 7, wherein the composition is added as a feed additive in an amount of 5.0 to 8.0%.
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