CN112930920A - Method for promoting growth of tissue culture seedlings of detoxified potatoes - Google Patents
Method for promoting growth of tissue culture seedlings of detoxified potatoes Download PDFInfo
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- CN112930920A CN112930920A CN202110249204.9A CN202110249204A CN112930920A CN 112930920 A CN112930920 A CN 112930920A CN 202110249204 A CN202110249204 A CN 202110249204A CN 112930920 A CN112930920 A CN 112930920A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The invention relates to the technical field of tissue culture, in particular to a method for promoting the growth of tissue culture seedlings of virus-free potatoes. According to the culture method, different artificial light sources are adopted for irradiation in different growth periods of the potato virus-free tissue culture seedling, so that the absorption rate and the utilization rate of the virus-free potato tissue culture seedling (test-tube seedling) to light energy can be effectively improved, the rapid differentiation of the virus-free potato callus is induced by light signals, the rapid growth of the potato virus-free tissue culture seedling is realized, the rapid propagation period is shortened, the multiplication coefficient is improved, and the quality of the tissue culture seedling is ensured.
Description
Technical Field
The invention relates to the technical field of tissue culture, in particular to a method for promoting the growth of tissue culture seedlings of virus-free potatoes.
Background
The illumination has a close relationship with the growth of the crops. The method captures the light energy to the maximum extent, fully exerts the potential of plant photosynthesis and is directly related to the benefit of agricultural production. The plant light source is a device which uses spectra with different wavelengths as light signals to stimulate plants to generate different endogenous metabolites according to the natural law of plant growth and the principle that the plants use sunlight to carry out photosynthesis, thereby promoting the physiological growth of the plants and providing the required light source for the plant growth.
The domestic plant light source market is rapidly developed in recent years, most plant light sources of LED manufacturing enterprises do not deeply research the spectrum with the highest plant utilization efficiency, and generally adopt full-visible continuous spectrum broadband LED equipment as the plant light source, so that most of the spectrum with lower plant utilization rate is wasted, the efficiency of converting electric energy into phytochemical energy is reduced, energy waste is caused, and the full-visible continuous light is adopted in the whole growth process, so that the plant can not grow rapidly.
The virus-free seedling (potato) is obtained by removing viruses in potato blocks through a series of technical measures, and has the advantages of early maturity, high yield, good quality and the like. In recent years, along with the popularization of planting areas, the production requirements of related seedlings (potatoes) also rapidly rise, and in order to accelerate the cultivation speed of the seedlings (potatoes), improve the quality of the seedlings (potatoes), reduce the use amount of exogenous hormones and change the growth light source environment of the seedlings (potatoes), the most effective and feasible mode is formed. However, at present, no report of promoting the rapid growth of the potato virus-free tissue culture seedlings by using an artificial light source is available.
Disclosure of Invention
In view of the above, the present invention provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes. The method adopts different artificial light sources to irradiate at different growth stages of the potato virus-free tissue culture seedling, realizes the rapid growth of the potato virus-free tissue culture seedling, shortens the rapid propagation period, improves the multiplication coefficient and ensures the quality of the tissue culture seedling.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for promoting the growth of detoxified potato tissue culture seedlings, which comprises the following steps of subculturing the detoxified potato tissue culture seedlings, and culturing the detoxified potato tissue culture seedlings under the irradiation of an artificial light source;
the artificial light source comprises, in light mass spectral integration percent: 50-80% of red light, 10-25% of blue light, 0-15% of green light, 0-10% of full visible light continuous spectrum and 0-3% of ultraviolet light, wherein the sum of the percentages is 100%;
the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the green light is 540-560nm, and the peak wavelength of the ultraviolet light is 280-320 nm.
In some embodiments, the artificial light source comprises a first artificial light source, a second artificial light source, and a third artificial light source;
performing a first group culture stage by adopting a first artificial light source 0-36h after the potato virus-free seedlings are subcultured; the first artificial light source comprises, in percent integrated photoplasmic spectrum: 65-80% of red light, 15-25% of blue light and 0-10% of full visible light continuous spectrum, wherein the sum of the percentages is 100%;
after the potato virus-free seedlings are subcultured for 36h and before hardening, a second artificial light source is adopted for carrying out a second tissue culture stage; the second artificial light source comprises, in percent of light mass spectral integration: 50-70% of red light, 10-20% of blue light, 0-15% of green light and 0-10% of full visible light continuous spectrum, wherein the sum of the percentages is 100%;
during hardening of the potato virus-free seedlings before transplanting, a third artificial light source is adopted for carrying out a third group of cultivation stages; the third artificial light source comprises, in percent of light mass spectral integration: 65-75% of red light, 15-25% of blue light, 0-10% of full visible light continuous spectrum and 0-3% of ultraviolet light, wherein the sum of the percentages is 100%.
The plant factory is a high-efficiency agricultural system for realizing annual continuous production of crops by high-precision environmental control in facilities, and utilizes an intelligent computer and an electronic sensing system to control the temperature, humidity, illumination and CO of plant growth2The concentration, nutrient solution and other environmental conditions are automatically controlled, so that the growth and development of plants in the facility are not or rarely restricted by natural conditions.
The culture method provided by the invention gives different artificial light sources in different growth periods of the potato virus-free tissue culture seedling, and the light energy obtained in the growth process of the potato virus-free tissue culture seedling is provided by the artificial light sources, and does not relate to direct solar light and scattered light, so that the culture method can be applied to the construction of plant tissue culture factories mainly using the artificial light sources.
In some embodiments, the first artificial light source comprises 65% of the red light, 25% of the blue light, 10% of the full visible continuum in integrated percentage of light mass spectrum; the second artificial light source comprises 62% of the red light, 15% of the blue light, 13% of the green light and 10% of full visible light continuous spectrum according to the integral percentage of light mass spectrum; the third artificial light source comprises 75% of the red light, 20% of the blue light, 3% of a full visible light continuous spectrum and 2% of ultraviolet light according to the integral percentage of a light mass spectrum;
wherein, in the first artificial light source, the peak wavelength of the red light is 660nm and the peak wavelength of the blue light is 480nm and 430;
in the second artificial light source, the peak wavelength of the red light is 660nm, the peak wavelength of the blue light is 480nm, and the peak wavelength of the green light is 560 nm;
in the third artificial light source, the peak wavelength of the red light is 660nm and the peak wavelength of the blue light is 480nm and the peak wavelength of the ultraviolet light is 320nm and the peak wavelength of the red light is 610 nm and the peak wavelength of the blue light is 430 nm and the peak wavelength of the ultraviolet light is 320nm and the peak wavelength of the blue light is 280 nm and the peak wavelength of the ultraviolet light.
In some embodiments, the first artificial light source is scattered light and the illumination intensity is from 40 to 60 μmol/(m)2S); in some embodiments, it is specifically 40. mu. mol/(m)2·s)。
In some embodiments, the second artificial light source comprises direct light and scattered light, and the illumination intensity is 60-120 [ mu ] mol/(m)2S). In some embodiments, it is specifically 120. mu. mol/(m)2·s)。
In some embodiments, the third artificial light source comprises direct light and scattered light, and the illumination intensity is 120-2S). In some embodiments, it is specifically 140. mu. mol/(m)2·s)。
In some embodiments, the illumination time (total illumination time/24 hours) of the first artificial light source and the second artificial light source is 14-16h/24h, and the illumination time (total illumination time/24 hours) of the third artificial light source is 12-14h/24 h. In some embodiments, the specific range may be 14h/24h, 16h/24h or 12h/24 h.
In some embodiments, the first and second culturing periods have a temperature of 18-25 ℃ and a humidity of 30-50%; the temperature of the third culture stage is 13-27 deg.C, and the humidity is 30-70%.
In some embodiments, the first, second and third culturing periods are at a temperature of 20-25 ℃ and a humidity of 30-50%. In some embodiments, the first, second and third culturing periods are at a temperature of 18-23 ℃ and a humidity of 40-50%. In some embodiments, the temperature of the first culturing stage is 18-23 deg.C and the humidity is 30-40%, the temperature of the second culturing stage is 20-23 deg.C and the humidity is 30-40%, and the temperature of the third culturing stage is 16-22 deg.C and the humidity is 50-60%.
The method for promoting the growth of the tissue culture seedlings of the detoxified potatoes provided by the invention comprises the following steps: subculturing the potato virus-free tissue culture seedlings, and culturing under the irradiation of an artificial light source; the artificial light source comprises, in light mass spectral integration percent: 50-80% of red light, 10-25% of blue light, 0-15% of green light, 0-10% of full visible light continuous spectrum and 0-3% of ultraviolet light, wherein the sum of the percentages is 100%; the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the green light is 540-560nm, and the peak wavelength of the ultraviolet light is 280-320 nm. Compared with the prior art, the invention has the following beneficial effects:
(1) the method provides a scientific and reasonable light source for cultivating the virus-free potato tissue culture seedlings (test tube seedlings) in a plant tissue culture factory, can effectively promote the rapid propagation growth of the virus-free potato tissue culture seedlings (test tube seedlings), and simultaneously improves the electric energy utilization rate, the stable yield and the quality of the virus-free potato tissue culture seedlings (test tube seedlings). The method covers a group of culture physiological growth periods of the virus-free potato tissue culture seedlings (test-tube seedlings) from growth to bottle formation after subculture inoculation, improves the absorption rate and the utilization rate of the virus-free potato tissue culture seedlings (test-tube seedlings) to light energy, induces the rapid differentiation of virus-free potato callus through light signals, promotes the rapid growth of the virus-free potato tissue culture seedlings (test-tube seedlings) under the full-artificial light environment, shortens the rapid propagation period, improves the multiplication coefficient and ensures the quality of tissue culture seedlings.
(2) The method for promoting the growth of the tissue culture seedlings of the virus-free potatoes has wide coverage range. According to the facility conditions of a plant tissue culture factory, the scientific and reasonable illumination scheme suitable for the rapid growth of the potato virus-free tissue culture seedlings is determined by combining the change conditions of the external temperature and humidity environment.
(3) The method for promoting the growth of the virus-free potato tissue culture seedling provided by the invention provides different light sources for different growth periods of the virus-free potato tissue culture seedling, has strong pertinence, and is suitable for the rapid propagation of the virus-free potato tissue culture seedling (test-tube seedling).
Detailed Description
The invention provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes. Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
The invention is further illustrated by the following examples:
example 1
The embodiment provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes, which is implemented in a plant factory with an artificial light source as a main light source, namely, all light energy obtained in the plant growth process is provided by the artificial light source and does not relate to direct solar light and scattered light; when the virus-free potato tissue culture seedlings (test-tube seedlings) are planted in the plant tissue culture factory, the artificial light source specifically comprises the following components:
the tissue culture seedling (test-tube seedling) of the detoxified potato is inoculated for 0-36h after subculture (the first group)A culture stage), a 36h later (a second culture stage) and a seedling exercising stage (a third culture stage), wherein the artificial light source comprises 70% of red light, 20% of blue light and 10% of full visible light continuous spectrum according to the integral percentage of light-mass spectrum, the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, and the total illumination intensity of the artificial light source in the first culture stage received by the top end of the plant is 40 mu mol/(m-480 nm)2S) and the total illumination intensity of the artificial light source in the second subculture stage is 80. mu. mol/(m)2S) direct light, the total illumination intensity of the artificial light source in the third cultivation stage being 170 [ mu ] mol/(m)2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory in all culture stages within 24 hours is 14 hours, the temperature of the plant growth environment is 20-25 ℃, and the humidity is 30-50%.
Example 2
The embodiment provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes, which is implemented in a plant factory with an artificial light source as a main light source, namely, all light energy obtained in the plant growth process is provided by the artificial light source and does not relate to direct solar light and scattered light; when the virus-free potato tissue culture seedlings (test-tube seedlings) are planted in the plant tissue culture factory, the artificial light source specifically comprises the following components:
within 0-36h (the first group culture stage) after the subculture of the tissue culture seedling (test-tube seedling) of the detoxified potato, the first artificial light source comprises 80% of red light and 20% of blue light according to the integral percentage of a light mass spectrum, the peak wavelength of the red light is 610-2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 15 hours, the temperature of the plant growth environment is 18-23 ℃, and the humidity is 40-50%;
after the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes are subcultured for 36h (second tissue culture stage), the second artificial light source comprises 57% of red light, 20% of blue light, 14% of green light and 9% of full visible light continuous spectrum according to the integral percentage of the light mass spectrum; the peak wavelength of the red light is610-2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 16 hours, the temperature of the plant growth environment is 18-23 ℃, and the humidity is 40-50%;
when the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes grow to bottle seedlings and are hardened (a third group culture stage), the third artificial light source comprises 65 percent of red light, 25 percent of blue light, 8 percent of full visible light continuous spectrum and 2 percent of ultraviolet light according to the integral percentage of the light mass spectrum; the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the ultraviolet light is 280-320nm, and the total illumination intensity of the third artificial light source received by the top end of the plant is 140 mu mol/(m)2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 14 hours, the temperature of the plant growth environment is 18-23 ℃, and the humidity is 40-50%.
Example 3
The embodiment provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes, which is implemented in a plant factory with an artificial light source as a main light source, namely, all light energy obtained in the plant growth process is provided by the artificial light source and does not relate to direct solar light and scattered light; when the virus-free potato tissue culture seedlings (test-tube seedlings) are planted in the plant tissue culture factory, the artificial light source specifically comprises the following components:
within 0-36h (the first group culture stage) after the subculture of the detoxified potato tissue culture seedling (test-tube seedling), the first artificial light source comprises 65% of red light, 25% of blue light and 10% of full visible light continuous spectrum according to the integral percentage of the light mass spectrum, the peak wavelength of the red light is 660nm, the peak wavelength of the blue light is 430-480nm, and the total illumination intensity of the first artificial light source received by the top end of the plant is 40 mu mol/(m-2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 14 hours, the temperature of the plant growth environment is 18-23 ℃, and the humidity is 30-40%;
after the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes are subcultured for 36h (second tissue culture stage), the second artificial light source comprises 66% of red light, 16% of blue light, 13% of green light and 5% of full visible light continuous spectrum according to the integral percentage of the light mass spectrum; the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the green light is 540-560nm, and the total illumination intensity of the second artificial light source received by the top end of the plant is 120 mu mol/(m)2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 14 hours, the temperature of the plant growth environment is 20-23 ℃, and the humidity is 30-40%;
when the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes grow to bottle seedlings and are hardened (a third group culture stage), the third artificial light source comprises 75% of red light, 20% of blue light, 3% of full visible light continuous spectrum and 2% of ultraviolet light according to the integral percentage of the light mass spectrum; the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the ultraviolet light is 280-320nm, and the total illumination intensity of the third artificial light source received by the top end of the plant is 160 mu mol/(m)2S), the accumulated illumination time of the artificial light source of the plant tissue culture factory within 24 hours is 12 hours, the temperature of the plant growth environment is 16-22 ℃, and the humidity is 50-60%.
Comparative example 1
The comparative example provides a method for promoting the growth of tissue culture seedlings of detoxified potatoes, which is the same as the conditions of the example 3 in a plant tissue culture factory which takes an artificial light source as a main light source, namely, all light energy obtained in the growth process of plants is provided by the artificial light source and does not relate to direct solar light and scattered light; when the virus-free potato tissue culture seedlings (test-tube seedlings) are planted in the plant tissue culture factory, the only difference is that the artificial light sources provided by the rapid propagation of the virus-free potato tissue culture seedlings (test-tube seedlings) are all full visible light continuous spectrums. The total illumination intensity and illumination time of the artificial light source received by the top of the plant, and the temperature and humidity of the culture environment are the same as those in embodiment 3.
Test example influence of different methods on growth of tissue culture seedlings of potatoes
And (3) performing tests according to the comparative example 1 and the examples 1-3, and observing the rapid propagation and growth conditions of the tissue culture seedlings (test-tube seedlings) of the virus-free potatoes.
The results show that:
the plant tissue culture factory in example 1, which uses an artificial light source as a main light source (i.e. all light energy obtained during the growth of the plant is provided by the artificial light source and does not relate to direct solar light and scattered light), cultures the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes, the seedlings (test-tube seedlings) can be hardened 18-21 days after inoculation, and the seedlings can grow normally after 7-14 days of hardening.
The plant tissue culture factory in example 2, which uses an artificial light source as a main light source (i.e. all light energy obtained during the growth of the plant is provided by the artificial light source and does not relate to direct solar light and scattered light), cultures the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes, the seedlings can be hardened 16-20 days after inoculation, and the seedlings can grow normally after 7-10 days of hardening.
The plant tissue culture factory in example 3, which uses an artificial light source as a main light source (i.e. all light energy obtained during the growth of the plant is provided by the artificial light source and does not relate to direct solar light and scattered light), cultures the tissue culture seedlings (test-tube seedlings) of the detoxified potatoes, the seedlings (test-tube seedlings) can be hardened 14-18 days after inoculation, and the seedlings can grow normally after 5-10 days of hardening.
Comparative example 1, in a plant tissue culture factory that uses an artificial light source as a main light source (i.e., the light energy obtained in the plant growth process is provided by the artificial light source and does not relate to direct solar light and scattered light), the artificial light sources provided in the rapid propagation growth of the detoxified potato tissue culture seedlings (tube seedlings) are all full visible light continuous spectra, the detoxified potato tissue culture seedlings (tube seedlings) can be acclimated 21-26 days after inoculation, and can grow normally after 7-14 days of acclimatization, and are transplanted 3-5 days later than the detoxified potato tissue culture seedlings (tube seedlings) of example 1, 5-10 days later than the detoxified potato tissue culture seedlings (tube seedlings) of example 2, and 9-12 days later than the detoxified potato tissue culture seedlings (tube seedlings) of example 3.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.
Claims (8)
1. A method for promoting the growth of tissue culture seedlings of detoxified potatoes is characterized in that the tissue culture seedlings of detoxified potatoes are subcultured and then cultured under the irradiation of an artificial light source;
the artificial light source comprises, in light mass spectral integration percent: 50-80% of red light, 10-25% of blue light, 0-15% of green light, 0-10% of full visible light continuous spectrum and 0-3% of ultraviolet light, wherein the sum of the percentages is 100%;
the peak wavelength of the red light is 610-660nm, the peak wavelength of the blue light is 430-480nm, the peak wavelength of the green light is 540-560nm, and the peak wavelength of the ultraviolet light is 280-320 nm.
2. The method of claim 1, wherein the artificial light source comprises a first artificial light source, a second artificial light source, and a third artificial light source;
performing a first group culture stage by adopting a first artificial light source 0-36h after the potato virus-free seedlings are subcultured; the first artificial light source comprises, in percent integrated photoplasmic spectrum: 65-80% of red light, 15-25% of blue light and 0-10% of full visible light continuous spectrum, wherein the sum of the percentages is 100%;
after the potato virus-free seedlings are subcultured for 36h and before hardening, a second artificial light source is adopted for carrying out a second tissue culture stage; the second artificial light source comprises, in percent of light mass spectral integration: 50-70% of red light, 10-20% of blue light, 0-15% of green light and 0-10% of full visible light continuous spectrum, wherein the sum of the percentages is 100%;
during hardening of the potato virus-free seedlings before transplanting, a third artificial light source is adopted for carrying out a third group of cultivation stages; the third artificial light source comprises, in percent of light mass spectral integration: 65-75% of red light, 15-25% of blue light, 0-10% of full visible light continuous spectrum and 0-3% of ultraviolet light, wherein the sum of the percentages is 100%.
3. The method of claim 2, wherein the first artificial light source comprises 65% red light, 25% blue light, 10% full visible continuous spectrum; the second artificial light source comprises 62% of red light, 15% of blue light, 13% of green light and 10% of full visible light continuous spectrum; the third artificial light source comprises 75% red light, 20% blue light, 3% full visible continuous spectrum and 2% ultraviolet light.
4. The method of claim 2 or 3, wherein the first artificial light source is scattered light and the illumination intensity is 40-60 μmol/(m)2·s)。
5. The method of claim 2 or 3, wherein the second artificial light source comprises direct light and scattered light, and the illumination intensity is 60-120 μmol/(m)2·s)。
6. The method as claimed in claim 2 or 3, wherein the third artificial light source comprises direct light and scattered light, and the illumination intensity is 120-2·s)。
7. The method as claimed in claim 2 or 3, wherein the illumination time of the first artificial light source and the second artificial light source is 14-16h/24h, and the illumination time of the third artificial light source is 12-14h/24 h.
8. The method according to claim 2 or 3, characterized in that the temperature of the first and second subculture stages is 18-25 ℃ and the humidity is 30-50%; the temperature of the third culture stage is 13-27 deg.C, and the humidity is 30-70%.
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| CN116406619A (en) * | 2023-04-28 | 2023-07-11 | 中国科学院长春应用化学研究所 | Light environment regulation and control method for accelerating expansion of potato tissue culture seedling tubers |
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