CN102726299A - Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source - Google Patents
Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source Download PDFInfo
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- CN102726299A CN102726299A CN2012102309063A CN201210230906A CN102726299A CN 102726299 A CN102726299 A CN 102726299A CN 2012102309063 A CN2012102309063 A CN 2012102309063A CN 201210230906 A CN201210230906 A CN 201210230906A CN 102726299 A CN102726299 A CN 102726299A
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Abstract
The invention discloses a rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source. The method adopts staged treatment with red/blue LED combined light source with adjustable light intensity: firstly monochromic red light (with main wavelength of 634 nm and spectrum range of 600-665 nm) is taken as China fir tissue culture light source to improve growth speed of tissue-cultured seedlings; then monochromic blue light (with main wavelength of 460 nm and spectrum range of 425-495 nm) is taken as light source to effectively promote rooting and seedling hardening. The method can improve China fir seedling growth speed and seedling robustness, shorten growth period, shorten rooting time, improve root number and vitality, improve transplantation survival rate, save electricity, improve China fir tissue-cultured seedling quality while greatly lower cost. The invention provides a novel method for rapid tissue culture propagation research of China fir.
Description
Technical field
The invention belongs to plant tissue culture fast breeding technique field, be specifically related to a kind of China fir two step method tissue culture and rapid propagation method based on led light source.
Background technology
The development of plant tissue culture fast breeding technique is day by day ripe so far; Yet also still there are problems; Particularly the research of China fir group culturation rapid propagating technology was in the stage of stagnation in recent years always; Fail to find one effectively can improve the tissue cultivating seedling quality, shorten the seedling growth cycle, the method that can reduce cost again; And the China fir group is trained the one side of taking root needs to improve the root system quality, shortens rootage duration, will reduce the interference of callus in the China fir rooting process on the other hand, so that improve transplanting survival rate.
Summary of the invention
The objective of the invention is to use the artificial light source of new LED (light-emitting diodes light) as the China fir tissue-culturing rapid propagation; Dividend, blue two kinds of monochromatic light shine step-by-step processing China fir tissue cultivating seedling stage by stage; Research obtains the light intensity combination of the most suitable China fir tissue-culturing rapid propagation; Confirm in the tissue-culturing rapid propagation process subculture and needed photochromic light intensity of stage such as take root, and the comprehensive function between photochromic; Final one side has effectively improved seedling growth speed and growth quality in nursery stock subculture process, improve the growth coefficient of nursery stock to greatest extent, effectively promotes it to take root on the other hand, promotes its strong sprout when taking root; Use growth rate and nursery stock robustness that this invention has not only improved the China fir seedling; Shorten growth cycle, and shortened rootage duration, increased root system bar number and root system vigor; Improved transplanting survival rate; And practiced thrift electric energy efficiently, thus comprehensive to a certain extent raising significantly reduced cost the quality of China fir tissue cultivating seedling the time, for the research of China fir tissue-culturing rapid propagation provides a new technology.
For realizing above-mentioned purpose, the present invention adopts following technical scheme:
The present invention adopts adjustable red, the blue led combined light source of light intensity, and the dominant wavelength of ruddiness is 634 nm, ranges 600-665 nm; The dominant wavelength of blue light is 460 nm, ranges 425-495 nm.
China fir tissue cultivating seedling of the present invention derives from the excellent Chinese fir clone seedling of Fujian China fir Engineering Technical Research Centre, and the experiment place is China fir Engineering Technical Research Centre comprehensive group training chamber, Fujian.Other conditions of group training chamber are identical with general China fir group training chamber.
The present invention is accomplished by following experimental procedure:
A, nursery stock subculture stage
(1) tissue cultivating seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: the subculture tissue cultivating seedling placed carry out successive transfer culture under the red LED light source, light treatment is 12 h/d; The tissue cultivating seedling that red light source is handled directly gets into takes root the stage, and the tissue cultivating seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness handled again;
B, nursery stock take root the stage
(1) the tissue cultivating seedling preparatory stage: be chosen at the good China fir clone seedling of successive transfer culture in the red LED illumination, be inoculated in the root media;
(2) light treatment: the tissue cultivating seedling of will taking root places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, refine seedling, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage is adopted is 634 nm, ranges 600-6605 nm, and range of light intensity is 60-160 μ mol/m
2S; The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, ranges 425-495 nm, and range of light intensity is 40-140 μ mol/m
2S; The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, ranges 425-495nm, and range of light intensity is 60-150 μ mol/m
2S.
Remarkable advantage of the present invention is: in the China fir tissue-culturing rapid propagation of reality, adopt said method to make and use the red LED light source to promote growth process in the nursery stock subculture stage; The blue led light source carries out rejuvenation to be handled; Effectively to different purposes the next stage light source of tissue cultivating seedling carried out classification processing; Have more scientific meaning, use processings of taking root of blue led light source in the nursery stock stage of taking root, when blue light promotion nursery stock takes root; Nursery stock had significant rejuvenation effect; Making for two steps handle can accomplish in a step, efficiently solves the drawback of nursery stock reduction in traditional nursery stock rooting process by contrast, and has reduced in the rooting process callus to the adverse effect of transplanting survival rate.A significance of the present invention is the consumption of significantly having practiced thrift electric energy; Artificial light source illumination aspect power consumption can be practiced thrift more than 2 times on the one hand; Improve seedling growth speed and rooting rate in addition, shortened the growth cycle of nursery stock, saved costs such as electric energy and medium; On the other hand, led light source is a cold light source, and it is less to produce heat in the lighting process, from and saved the air-conditioning power consumption of adjusting group training room temperature.
Embodiment
Embodiment 1
A, nursery stock subculture stage
(1) tissue cultivating seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: the subculture tissue cultivating seedling placed carry out successive transfer culture under the red LED light source, light treatment is 12 h/d; The tissue cultivating seedling that red light source is handled directly gets into takes root the stage, and the tissue cultivating seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness handled again;
B, nursery stock take root the stage
(1) the tissue cultivating seedling preparatory stage: be chosen at the good China fir clone seedling of successive transfer culture in the red LED illumination, be inoculated in the root media;
(2) light treatment: the tissue cultivating seedling of will taking root places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, refine seedling, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage is adopted is 634nm, ranges 600-665nm, and light intensity is 60 μ mol/m
2S; The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, ranges 425-495 nm, and light intensity is 40 μ mol/m
2S; The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, ranges 425-495 nm, and light intensity is 60 μ mol/m
2S.
Embodiment 2
A, nursery stock subculture stage
(1) tissue cultivating seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: the subculture tissue cultivating seedling placed carry out successive transfer culture under the red LED light source, light treatment is 12 h/d; The tissue cultivating seedling that red light source is handled can directly get into the stage of taking root, and the tissue cultivating seedling that needs the continuation subculture then is after ruddiness is handled, and places the blue led light source to carry out rejuvenation again and handles;
B, nursery stock take root the stage
(1) the tissue cultivating seedling preparatory stage: be chosen at the good China fir clone seedling of successive transfer culture in the red LED illumination, be inoculated in the root media;
(2) light treatment: the tissue cultivating seedling of will taking root places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, refine seedling, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage is adopted is 634 nm, ranges 600-665 nm, and light intensity is 110 μ mol/m
2S; The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, ranges 425-495 nm, and light intensity is 90 μ mol/m
2S; The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, ranges 425-495 nm, and light intensity is 100 μ mol/m
2S.
Embodiment 3
A, nursery stock subculture stage
(1) tissue cultivating seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: the subculture tissue cultivating seedling placed carry out successive transfer culture under the red LED light source, light treatment is 12 h/d; The tissue cultivating seedling that red light source is handled directly gets into takes root the stage, and the tissue cultivating seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness handled again;
B, nursery stock take root the stage
(1) the tissue cultivating seedling preparatory stage: be chosen at the good China fir clone seedling of successive transfer culture in the red LED illumination, be inoculated in the root media;
(2) light treatment: the tissue cultivating seedling of will taking root places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, refine seedling, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage is adopted is 634 nm, ranges 600-665nm, and light intensity is 160 μ mol/m
2S; The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, ranges 425-495 nm, and light intensity is 140 μ mol/m
2S; The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, ranges 425-495 nm, and light intensity is 150 μ mol/m
2S.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. based on the China fir two step method tissue culture and rapid propagation method of led light source, it is characterized in that: utilize the artificial light source of light emitting diode as the China fir tissue-culturing rapid propagation.
2. the China fir two step method tissue culture and rapid propagation method based on led light source according to claim 1 is characterized in that: with color red, blue led light source stage by stage substep combination apply in the China fir tissue-culturing rapid propagation.
3. the China fir two step method tissue culture and rapid propagation method based on led light source according to claim 1 is characterized in that: adopt following steps to carry out tissue-culturing rapid propagation:
A, nursery stock subculture stage
(1) tissue cultivating seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: the subculture tissue cultivating seedling placed carry out successive transfer culture under the red LED light source, light treatment is 12 h/d; The tissue cultivating seedling that red light source is handled directly gets into takes root the stage, and the tissue cultivating seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness handled again;
B, nursery stock take root the stage
(1) the tissue cultivating seedling preparatory stage: be chosen at the good China fir clone seedling of successive transfer culture in the red LED illumination, be inoculated in the root media;
Light treatment: the tissue cultivating seedling of will taking root places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, refine seedling, carry out outdoor transplanting then.
4. the China fir two step method tissue culture and rapid propagation method based on led light source according to claim 3, it is characterized in that: the red LED dominant wavelength that the subculture stage is adopted is 634 nm, ranges 600-665 nm, range of light intensity is 60-160 μ mol/m
2S; The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, ranges 425-495 nm, and range of light intensity is 40-140 μ mol/m
2S; The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, ranges 425-495 nm, and range of light intensity is 60-150 μ mol/m
2S.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103650928A (en) * | 2013-11-26 | 2014-03-26 | 大连好地农业有限公司 | Grafted seedling callusing system |
| CN104429956A (en) * | 2014-11-24 | 2015-03-25 | 广西大学 | Method for test-tube rooting of cunninghamia lanceolata tissue culture seedlings by using compound LED light source |
| CN104686326A (en) * | 2015-02-13 | 2015-06-10 | 福建农林大学 | Method for promoting rooting of China fir tissue culture seedling |
| CN107593452A (en) * | 2017-11-02 | 2018-01-19 | 樊仲书 | A kind of cultural method of China fir tissue-cultured seedling fast breeding |
| CN107637464A (en) * | 2017-11-02 | 2018-01-30 | 樊昌华 | Induction succulent propagation and the method taken root are handled using light sources with different wavelengths |
| RU2745449C1 (en) * | 2020-09-28 | 2021-03-25 | Автономная некоммерческая организация «Институт социально-экономических стратегий и технологий развития» | Method for activating germination of seeds of cereal meadow grass |
| CN112930920A (en) * | 2021-03-08 | 2021-06-11 | 中国科学院长春应用化学研究所 | Method for promoting growth of tissue culture seedlings of detoxified potatoes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104429956A (en) * | 2014-11-24 | 2015-03-25 | 广西大学 | Method for test-tube rooting of cunninghamia lanceolata tissue culture seedlings by using compound LED light source |
| CN104686326A (en) * | 2015-02-13 | 2015-06-10 | 福建农林大学 | Method for promoting rooting of China fir tissue culture seedling |
| CN107593452A (en) * | 2017-11-02 | 2018-01-19 | 樊仲书 | A kind of cultural method of China fir tissue-cultured seedling fast breeding |
| CN107637464A (en) * | 2017-11-02 | 2018-01-30 | 樊昌华 | Induction succulent propagation and the method taken root are handled using light sources with different wavelengths |
| RU2745449C1 (en) * | 2020-09-28 | 2021-03-25 | Автономная некоммерческая организация «Институт социально-экономических стратегий и технологий развития» | Method for activating germination of seeds of cereal meadow grass |
| CN112930920A (en) * | 2021-03-08 | 2021-06-11 | 中国科学院长春应用化学研究所 | Method for promoting growth of tissue culture seedlings of detoxified potatoes |
| CN112930920B (en) * | 2021-03-08 | 2023-06-20 | 中国科学院长春应用化学研究所 | Method for promoting growth of detoxified potato tissue culture seedlings |
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