CN104686326A - Method for promoting rooting of China fir tissue culture seedling - Google Patents
Method for promoting rooting of China fir tissue culture seedling Download PDFInfo
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- CN104686326A CN104686326A CN201510076461.1A CN201510076461A CN104686326A CN 104686326 A CN104686326 A CN 104686326A CN 201510076461 A CN201510076461 A CN 201510076461A CN 104686326 A CN104686326 A CN 104686326A
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- plantlet
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- china fir
- rooting
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Abstract
The invention provides a method for promoting rooting of a China fir tissue culture seedling and belongs to the technical field of plant tissue culture and rapid propagation. By using a 28W fluorescent lamp of PAK, the illumination intensity is 1000-2000Lux, the illumination period is 12h/d. A near ultraviolet lamp with the spectra of 370-390nm and the illumination intensity of 50-100 micro-mol/m<2>.s is adopted for auxiliary irradiation treatment for 1-3 h/d. By applying the method provided by the invention, not only can the rooting effect of the China fir be improved and the rooting quantity increased, but also the root system quality is enhanced, and moreover, the weight of the overground part of the China fir tissue culture seedling can be effectively improved so as to enhance the quality of the seedling, thereby providing method for rooting research on China fir tissue culture and rapid propagation.
Description
Technical field
The invention belongs to plant tissue culture fast breeding technique field, be specifically related to a kind of near ultraviolet ray LED light source that utilizes as the China fir plantlet in vitro rooting method of secondary light source.
Background technology
China fir is as one of most important commerical tree species of south China, be distributed in south China 15 provinces and regions, to the national timber of guarantee, there is safely important strategic importance, the attention degree of building for forest plantation along with this country is in recent years more and more higher, therefore market is also increasing for the demand of China Fir Seedling, traditional seedling raising manners cannot be met the need of market gradually, therefore many scholar's research explore China fir group culturation rapid propagating technology in recent years, and form a set of comparatively ripe method for tissue culture.Take root as one of most important step in the fast numerous process of plant tissue culture, the effect of taking root is by the early growth situation of the transplanting survival rate and seedling that directly have influence on plant.Therefore take root in research process in China fir tissue-culturing rapid propagation process, on the basis ensureing seedling quality, rooting efficiency should be able to be promoted to greatest extent, thus shortening is taken root the cycle on the one hand, reduction group training production cost; The healthy growth of seedling replanting survival rate and guarantee forestation of China fir process Fast-growth phase can be improved on the other hand.
The photomorphogenesis of blue light involved in plant, can impel plant to react to adverse circumstance, and existing a lot of research shows that blue light can all have facilitation in various degree to the rooting efficiency of a lot of plant.And black light can impel plant dwarfing equally, make its blade thickening, there is certain effect in strong sprout, to the root growth of Activities of Some Plants, there is facilitation simultaneously.
The information being disclosed in this background technology part is only intended to increase the understanding to general background of the present invention, and should not be regarded as admitting or imply in any form that this information structure has been prior art that persons skilled in the art are known.
Summary of the invention
Main purpose of the present invention uses near ultraviolet LED (light emitting diode) light source as indirect labor's light source of China fir tissue-culturing rapid propagation culture of rootage, point different spectrum, varying strength and the near ultraviolet light treatment China fir of different disposal time are taken root plantlet in vitro, and research is conducive to China fir tissue-culturing rapid propagation most and takes root stage the most applicable nearly purple light spectrum, light intensity and processing time; Make it can effectively promote on the one hand and promote that China fir plantlet in vitro is taken root, on the other hand there is certain effect in strong sprout.Application the present invention not only can improve the rooting efficiency of Chinese Fir Seedling, increase number of taking root, and promote root system quality, effectively can improve China fir plantlet in vitro acrial part weight simultaneously, then seedling quality is promoted, for the research of taking root of China fir tissue-culturing rapid propagation provides a kind of method.
For achieving the above object, the present invention adopts following technical scheme:
The present invention is the ordinary light source adopted is the fluorescent lamp that the male aurora of 28 w tri-throw light on, and secondary light source is near ultraviolet LED light source, and spectral range is 370 ~ 390 nm.
China fir plantlet in vitro of the present invention derives from the excellent Chinese fir clone seedling of State Administration of Forestry's China fir Engineering Technical Research Centre, and experiment place is positioned at State Administration of Forestry's China fir Engineering Technical Research Centre group training room.The fluorescent lamp that light source is three male aurora illuminations is cultivated in group training room, and periodicity of illumination is 12 h/d, and intensity of illumination is 1000 ~ 2000 Lux.
The present invention completes mainly through following experimental procedure:
1, the plantlet in vitro preparatory stage
Squamous subculture is carried out to excellent Clones of Cunninghamia Lanceolata plantlet in vitro, when being cultured to 2 ~ 4cm, carries out corresponding rooting treatment;
2, plantlet in vitro rooting treatment
Choose the squamous subculture Clones of Cunninghamia Lanceolata seedling of 2 ~ 4 cm effects, be inoculated in root media and carry out culture of rootage, cultivate the fluorescent lamp that light source is the male aurora illumination of 28 w tri-, intensity of illumination is 1000 ~ 2000 Lux, periodicity of illumination is 12 h/d, and it is 50 ~ 100 μm of ol/m that experimental group adopts spectrum to be 370 ~ 390 nm intensities of illumination
2the near ultraviolet lamp auxiliary irradiation process of s, the processing time is 1 ~ 3 h/d, closes fluorescent lamp during near ultraviolet light treatment, and control group adopts the fluorescent lamp of 12 h/d to continue process;
3, interpretation
Measure China fir plantlet in vitro condition of rooting after rooting treatment 45 d, result shows: adopt the China fir plantlet in vitro individual plant of black light aid in treatment number of on average taking root to be 8.45 ~ 9.36, fluorescent lamp control group is 8.27; The average root system fresh weight of China fir plantlet in vitro individual plant adopting black light aid in treatment is 0.191 ~ 0.228 g, and fluorescent lamp control group is 0.190 g; The China fir individual plant plantlet in vitro acrial part fresh weight adopting black light aid in treatment is 0.109 ~ 0.125 g, and fluorescent lamp control group is 0.101g; On average take root number, the average root system fresh weight of individual plant and individual plant plantlet in vitro acrial part fresh weight of the individual plant of near ultraviolet aid in treatment group China fir plantlet in vitro improves 2.18% ~ 13.06%, 0.53% ~ 20.00% and 7.92% ~ 23.76% respectively compared with fluorescent lamp control group.
Remarkable advantage of the present invention is: in use conventional fluorescent as taking root on the basis of light source, black light is utilized plant to be had to the effect downgraded strong sprout and promote root growth, but to plant, there is lethal effect when the wavelength of black light is too low or the processing time is long simultaneously, therefore explore and select suitable wavelength, intensity and the near ultraviolet light source in processing time to carry out aid in treatment, can effectively promote its root growth while guarantee seedling healthy growth, have more scientific meaning.With the take root holy radical amount of China fir plantlet in vitro compared with mode, root system quality and above-ground plant parts of tradition, there is increase in various degree or optimization, thus improve seedling replanting survival rate and ensure the healthy growth of forestation of China fir process Fast-growth phase.In addition, the present invention adopts LED light source as secondary light source, the consumption of electric energy has significantly been saved compared with the fluorescent lamp process of conventional persistence, improve the quality of rooting of seedling in addition, be equivalent to indirectly shorten rootage duration, therefore, it is possible to improve the speed of production of seedling on the basis ensureing seedling quality.
Embodiment
embodiment 1
1, the plantlet in vitro preparatory stage
Squamous subculture is carried out to excellent Clones of Cunninghamia Lanceolata plantlet in vitro, when being cultured to 3cm, carries out corresponding rooting treatment;
2, plantlet in vitro rooting treatment
Choose the squamous subculture Clones of Cunninghamia Lanceolata seedling of 3 cm effects, be inoculated in root media and carry out culture of rootage, cultivate the fluorescent lamp that light source is the male aurora illumination of 28 w tri-, intensity of illumination is 1500 Lux, periodicity of illumination is 12 h/d, and it is 70 μm of ol/m that experimental group adopts spectrum to be 375 nm intensities of illumination
2the near ultraviolet lamp auxiliary irradiation process of s, the processing time is 1.5 h/d, closes fluorescent lamp during near ultraviolet light treatment, and control group adopts the fluorescent lamp of 12 h/d to continue process;
3, interpretation
Measure China fir plantlet in vitro condition of rooting after rooting treatment 45 d, result shows: adopt the China fir plantlet in vitro individual plant of black light aid in treatment number of on average taking root to be 8.83, fluorescent lamp control group is 8.27; The average root system fresh weight of China fir plantlet in vitro individual plant adopting black light aid in treatment is 0.194 g, and fluorescent lamp control group is 0.190 g; The China fir individual plant plantlet in vitro acrial part fresh weight adopting black light aid in treatment is 0.109 g, and fluorescent lamp control group is 0.101g; On average take root number, the average root system fresh weight of individual plant and individual plant plantlet in vitro acrial part fresh weight of the individual plant of near ultraviolet aid in treatment group China fir plantlet in vitro improves 6.77%, 2.11% and 7.92% respectively compared with fluorescent lamp control group.
embodiment 2
1, the plantlet in vitro preparatory stage
Squamous subculture is carried out to excellent Clones of Cunninghamia Lanceolata plantlet in vitro, when being cultured to 4cm, carries out corresponding rooting treatment;
2, plantlet in vitro rooting treatment
Choose the squamous subculture Clones of Cunninghamia Lanceolata seedling of 4 cm effects, be inoculated in root media and carry out culture of rootage, cultivate the fluorescent lamp that light source is the male aurora illumination of 28 w tri-, intensity of illumination is 2000 Lux, periodicity of illumination is 12 h/d, and it is 70 μm of ol/m that experimental group adopts spectrum to be 385 nm intensities of illumination
2the near ultraviolet lamp auxiliary irradiation process of s, the processing time is 2 h/d, closes fluorescent lamp during near ultraviolet light treatment, and control group adopts the fluorescent lamp of 12 h/d to continue process;
3, interpretation
Measure China fir plantlet in vitro condition of rooting after rooting treatment 45 d, result shows: adopt the China fir plantlet in vitro individual plant of black light aid in treatment number of on average taking root to be 9.20, fluorescent lamp control group is 8.27; The average root system fresh weight of China fir plantlet in vitro individual plant adopting black light aid in treatment is 0.223 g, and fluorescent lamp control group is 0.190 g; The China fir individual plant plantlet in vitro acrial part fresh weight adopting black light aid in treatment is 0.120 g, and fluorescent lamp control group is 0.101g; On average take root number, the average root system fresh weight of individual plant and individual plant plantlet in vitro acrial part fresh weight of the individual plant of near ultraviolet aid in treatment group China fir plantlet in vitro improves 11.25%, 17.37% and 18.81% respectively compared with fluorescent lamp control group.
The foregoing is only preferred embodiment of the present invention, effect is in order to illustrate and illustration, the object selected exemplary embodiment and describe is to explain certain principles of the present invention and practical application thereof, thus those skilled in the art can be realized and utilize various different exemplary of the present invention and various different selection and change.All equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. promote to it is characterized in that the method that China fir plantlet in vitro is taken root: described method comprises the steps:
1) the plantlet in vitro preparatory stage
Squamous subculture is carried out to excellent Clones of Cunninghamia Lanceolata plantlet in vitro, when being cultured to 2 ~ 4cm, carries out corresponding rooting treatment;
2) plantlet in vitro rooting treatment
Choose the squamous subculture Clones of Cunninghamia Lanceolata seedling of 2 ~ 4 cm effects, be inoculated in root media and carry out culture of rootage, cultivate light source and adopt ordinary light source to be the fluorescent lamp that the male aurora of 28 w tri-throw light on, secondary light source is near ultraviolet LED light source;
3) interpretation
China fir plantlet in vitro condition of rooting is measured after rooting treatment 45 d.
2. a kind of method that China fir plantlet in vitro is taken root that promotes according to claim 1, it is characterized in that: described cultivation light source is specially the fluorescent lamp of the male aurora illumination of 28 w tri-, intensity of illumination is 1000 ~ 2000 Lux, periodicity of illumination is 12 h/d, and adopting spectrum to be 370 ~ 390 nm intensities of illumination is 50 ~ 100 μm of ol/m
2the near ultraviolet lamp auxiliary irradiation process of s, the processing time is 1 ~ 3 h/d, closes fluorescent lamp during near ultraviolet light treatment.
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| CN201510076461.1A CN104686326A (en) | 2015-02-13 | 2015-02-13 | Method for promoting rooting of China fir tissue culture seedling |
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| CN201510076461.1A CN104686326A (en) | 2015-02-13 | 2015-02-13 | Method for promoting rooting of China fir tissue culture seedling |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660416A (en) * | 2016-03-16 | 2016-06-15 | 蒋凡 | Root induction method for Chinese fir test-tube plantlets |
| CN109362392A (en) * | 2018-12-26 | 2019-02-22 | 厦门大学 | A kind of illumination apparatus effectively facilitating arabidopsis nutrient growth |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217539A (en) * | 2011-05-19 | 2011-10-19 | 南京林业大学 | Isolated rooting culture method for fir clone |
| CN102726299A (en) * | 2012-07-05 | 2012-10-17 | 福建农林大学 | Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source |
| CN103229723A (en) * | 2013-05-23 | 2013-08-07 | 广西壮族自治区林业科学研究院 | Rooting induction method of test-tube plantlet of cunninghamia lanceolata |
-
2015
- 2015-02-13 CN CN201510076461.1A patent/CN104686326A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217539A (en) * | 2011-05-19 | 2011-10-19 | 南京林业大学 | Isolated rooting culture method for fir clone |
| CN102726299A (en) * | 2012-07-05 | 2012-10-17 | 福建农林大学 | Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source |
| CN103229723A (en) * | 2013-05-23 | 2013-08-07 | 广西壮族自治区林业科学研究院 | Rooting induction method of test-tube plantlet of cunninghamia lanceolata |
Non-Patent Citations (3)
| Title |
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| MU-LAN ZHU ETAL: "Efficient organogenesis and plantlet regeneration in the timber species Cunninhhamia lanceolata(Lamb.)Hook", 《INVITROCELL.DEV.BIOL.—PLANT》 * |
| 付婷等: "速生广西良种杉木组培快繁技术初步研究", 《北方园艺》 * |
| 苏治南等: "不同生根剂对杉木扦插根形态建成的影响", 《基因组学与应用生物学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660416A (en) * | 2016-03-16 | 2016-06-15 | 蒋凡 | Root induction method for Chinese fir test-tube plantlets |
| CN109362392A (en) * | 2018-12-26 | 2019-02-22 | 厦门大学 | A kind of illumination apparatus effectively facilitating arabidopsis nutrient growth |
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Application publication date: 20150610 |