CN102726299B - Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source - Google Patents
Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source Download PDFInfo
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- CN102726299B CN102726299B CN 201210230906 CN201210230906A CN102726299B CN 102726299 B CN102726299 B CN 102726299B CN 201210230906 CN201210230906 CN 201210230906 CN 201210230906 A CN201210230906 A CN 201210230906A CN 102726299 B CN102726299 B CN 102726299B
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Abstract
The invention discloses a rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source. The method adopts staged treatment with red/blue LED combined light source with adjustable light intensity: firstly monochromic red light (with main wavelength of 634 nm and spectrum range of 600-665 nm) is taken as China fir tissue culture light source to improve growth speed of tissue-cultured seedlings; then monochromic blue light (with main wavelength of 460 nm and spectrum range of 425-495 nm) is taken as light source to effectively promote rooting and seedling hardening. The method can improve China fir seedling growth speed and seedling robustness, shorten growth period, shorten rooting time, improve root number and vitality, improve transplantation survival rate, save electricity, improve China fir tissue-cultured seedling quality while greatly lower cost. The invention provides a novel method for rapid tissue culture propagation research of China fir.
Description
Technical field
The invention belongs to plant tissue culture fast breeding technique field, be specifically related to a kind of China fir two step method tissue culture and rapid propagation method based on led light source.
Background technology
The development of plant tissue culture fast breeding technique is day by day ripe so far, yet also still there are problems, particularly the research of China fir group culturation rapid propagating technology was in the stage of stagnation in recent years always, fail to find one effectively can the raising group train the seedling quality, shorten seedling growth cycle, the method that can reduce cost again; And the training of China fir group is taken root needs to improve the root system quality on the one hand, shortens rootage duration, will reduce the interference of callus in the China fir rooting process on the other hand, in order to improve transplanting survival rate.
Summary of the invention
The objective of the invention is to use new LED (light-emitting diodes light) as the artificial light source of China fir tissue-culturing rapid propagation, dividend, blue two kinds of monochromatic light shine step-by-step processing China fir group training seedling stage by stage, research obtains the light intensity combination of the most suitable China fir tissue-culturing rapid propagation, needed photochromic light intensity of stage such as determine in the tissue-culturing rapid propagation process subculture and take root, and the comprehensive function between photochromic; Final one side has effectively improved seedling growth speed and growth quality in nursery stock subculture process, improve the growth coefficient of nursery stock to greatest extent, effectively promotes it to take root on the other hand, promotes its strong sprout when taking root; Use growth rate and nursery stock robustness that this invention has not only improved the China fir seedling, shortened growth cycle, and shortened rootage duration, root system bar number and root system vigor have been increased, improved transplanting survival rate, and saved electric energy efficiently, thus to a certain extent comprehensive raising significantly reduced cost the quality of China fir group training seedling the time, for the research of China fir tissue-culturing rapid propagation provides a new technology.
For achieving the above object, the present invention adopts following technical scheme:
The present invention adopts adjustable red, the blue led combined light source of light intensity, and the dominant wavelength of ruddiness is 634 nm, wave spectrum scope 600-665 nm; The dominant wavelength of blue light is 460 nm, wave spectrum scope 425-495 nm.
China fir group training seedling of the present invention derives from the excellent Chinese fir clone seedling of Fujian China fir Engineering Technical Research Centre, and the experiment place is that Fujian China fir Engineering Technical Research Centre is comprehensively organized the training chamber.Other conditions of group training chamber are identical with general China fir group training chamber.
The present invention is finished by following experimental procedure:
A, nursery stock subculture stage
(1) group training seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: subculture group training seedling placed carry out subculture under the red LED light source and cultivate, light treatment is 12 h/d; The group training seedling that red light source is handled directly enters the stage of taking root, and the group training seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness be handled again;
B, nursery stock take root the stage
(1) the group training seedling preparatory stage: be chosen at the red LED illumination relaying foster good China fir clone seedling of being commissioned to train, be inoculated in the root media;
(2) light treatment: the group of will taking root training seedling places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, carry out hardening, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage adopts is 634 nm, wave spectrum scope 600-6605 nm, and range of light intensity is 60-160 μ mol/(m
2S); The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, wave spectrum scope 425-495 nm, and range of light intensity is 40-140 μ mol/(m
2S); The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, wave spectrum scope 425-495nm, and range of light intensity is 60-150 μ mol/(m
2S).
Remarkable advantage of the present invention is: adopt said method to make in the China fir tissue-culturing rapid propagation of reality and use the red LED light source to promote growth process in the nursery stock subculture stage, the blue led light source carries out rejuvenation to be handled, effectively to different purposes the next stage light source of group training seedling carried out classification and handled, have more scientific meaning, use the processing of taking root of blue led light source in the nursery stock stage of taking root, when blue light promotes that nursery stock takes root, nursery stock had significant rejuvenation effect, making for two steps handle can finish in a step, efficiently solve the drawback of the reduction of nursery stock in traditional nursery stock rooting process by contrast, and reduced in the rooting process callus to the adverse effect of transplanting survival rate.A significance of the present invention is the consumption of significantly having saved electric energy, artificial light source illumination aspect power consumption can be saved more than 2 times on the one hand, improve seedling growth speed and rooting rate in addition, shortened the growth cycle of nursery stock, saved costs such as electric energy and medium; On the other hand, led light source is cold light source, and it is less to produce heat in the lighting process, from and saved the air-conditioning power consumption of adjusting group training room temperature.
Embodiment
Embodiment 1
A, nursery stock subculture stage
(1) group training seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: subculture group training seedling placed carry out subculture under the red LED light source and cultivate, light treatment is 12 h/d; The group training seedling that red light source is handled directly enters the stage of taking root, and the group training seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness be handled again;
B, nursery stock take root the stage
(1) the group training seedling preparatory stage: be chosen at the red LED illumination relaying foster good China fir clone seedling of being commissioned to train, be inoculated in the root media;
(2) light treatment: the group of will taking root training seedling places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, carry out hardening, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage adopts is 634nm, wave spectrum scope 600-665nm, and light intensity is 60 μ mol/(m
2S); The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 40 μ mol/(m
2S); The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 60 μ mol/(m
2S).
Embodiment 2
A, nursery stock subculture stage
(1) group training seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: subculture group training seedling placed carry out subculture under the red LED light source and cultivate, light treatment is 12 h/d; The group training seedling that red light source is handled can directly enter the stage of taking root, and the group training Miao Zeshi that needs to continue subculture places the blue led light source to carry out rejuvenation again and handles after ruddiness is handled;
B, nursery stock take root the stage
(1) the group training seedling preparatory stage: be chosen at the red LED illumination relaying foster good China fir clone seedling of being commissioned to train, be inoculated in the root media;
(2) light treatment: the group of will taking root training seedling places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, carry out hardening, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage adopts is 634 nm, wave spectrum scope 600-665 nm, and light intensity is 110 μ mol/(m
2S); The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 90 μ mol/(m
2S); The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 100 μ mol/(m
2S).
Embodiment 3
A, nursery stock subculture stage
(1) group training seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: subculture group training seedling placed carry out subculture under the red LED light source and cultivate, light treatment is 12 h/d; The group training seedling that red light source is handled directly enters the stage of taking root, and the group training seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness be handled again;
B, nursery stock take root the stage
(1) the group training seedling preparatory stage: be chosen at the red LED illumination relaying foster good China fir clone seedling of being commissioned to train, be inoculated in the root media;
(2) light treatment: the group of will taking root training seedling places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches about 2-4 cm, carry out hardening, carry out outdoor transplanting then.
The red LED dominant wavelength that the subculture stage adopts is 634 nm, wave spectrum scope 600-665nm, and light intensity is 160 μ mol/(m
2S); The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 140 μ mol/(m
2S); The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, wave spectrum scope 425-495 nm, and light intensity is 150 μ mol/(m
2S).
The above only is preferred embodiment of the present invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (1)
1. based on the China fir two step method tissue culture and rapid propagation method of led light source, it is characterized in that: utilize light emitting diode as the artificial light source of China fir tissue-culturing rapid propagation;
With red, the blue led light source of monochrome stage by stage substep combination apply in the China fir tissue-culturing rapid propagation;
Adopt following steps to carry out tissue-culturing rapid propagation:
A, nursery stock subculture stage
(1) group training seedling preparation: select the stem apex of good China fir clonal tissue culture seedling partly to be inoculated in the subculture medium;
(2) light treatment: subculture group training seedling placed carry out subculture under the red LED light source and cultivate, light treatment is 12 h/d; The group training seedling that red light source is handled directly enters the stage of taking root, and the group training seedling that needs to continue subculture need place the blue led light source to carry out rejuvenation and handle after ruddiness be handled again;
B, nursery stock take root the stage
(1) the group training seedling preparatory stage: be chosen at the red LED illumination relaying foster good China fir clone seedling of being commissioned to train, be inoculated in the root media;
(2) light treatment: the group of will taking root training seedling places the blue led light source to carry out culture of rootage, and light application time is 12 h/d;
C, nursery stock transplanting stage
When the seedling root average length of taking root reaches 2-4 cm, carry out hardening, carry out outdoor transplanting then;
The red LED dominant wavelength that the subculture stage adopts is 634 nm, wave spectrum scope 600-665 nm, and range of light intensity is 60-160 μ mol/(m
2S); The dominant wavelength of the blue led light source that the rejuvenation of subculture stage is used is 460 nm, wave spectrum scope 425-495 nm, and range of light intensity is 40-140 μ mol/(m
2S); The dominant wavelength of the blue led light source that the stage of taking root adopts is 460 nm, wave spectrum scope 425-495 nm, and range of light intensity is 60-150 μ mol/(m
2S).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210230906 CN102726299B (en) | 2012-07-05 | 2012-07-05 | Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source |
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| CN 201210230906 CN102726299B (en) | 2012-07-05 | 2012-07-05 | Rapid two-step tissue culture propagation method of China fir based on LED (light emitting diode) light source |
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| CN102726299A CN102726299A (en) | 2012-10-17 |
| CN102726299B true CN102726299B (en) | 2013-08-07 |
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| CN103650928A (en) * | 2013-11-26 | 2014-03-26 | 大连好地农业有限公司 | Grafted seedling callusing system |
| CN104429956A (en) * | 2014-11-24 | 2015-03-25 | 广西大学 | Method for test-tube rooting of cunninghamia lanceolata tissue culture seedlings by using compound LED light source |
| CN104686326A (en) * | 2015-02-13 | 2015-06-10 | 福建农林大学 | Method for promoting rooting of China fir tissue culture seedling |
| CN107637464A (en) * | 2017-11-02 | 2018-01-30 | 樊昌华 | Induction succulent propagation and the method taken root are handled using light sources with different wavelengths |
| CN107593452A (en) * | 2017-11-02 | 2018-01-19 | 樊仲书 | A kind of cultural method of China fir tissue-cultured seedling fast breeding |
| RU2745449C1 (en) * | 2020-09-28 | 2021-03-25 | Автономная некоммерческая организация «Институт социально-экономических стратегий и технологий развития» | Method for activating germination of seeds of cereal meadow grass |
| CN112930920B (en) * | 2021-03-08 | 2023-06-20 | 中国科学院长春应用化学研究所 | Method for promoting growth of detoxified potato tissue culture seedlings |
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