CN112891473A - Deer heart blood composition helpful for improving anoxia tolerance - Google Patents

Deer heart blood composition helpful for improving anoxia tolerance Download PDF

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CN112891473A
CN112891473A CN202110182738.4A CN202110182738A CN112891473A CN 112891473 A CN112891473 A CN 112891473A CN 202110182738 A CN202110182738 A CN 202110182738A CN 112891473 A CN112891473 A CN 112891473A
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composition
freeze
deer
parts
cordyceps militaris
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杨钧
吴存虎
唐静
南敏伦
赫玉芳
赵全成
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Hebei Lubao Bio Engineering Co ltd
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Hebei Lubao Bio Engineering Co ltd
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Abstract

The invention relates to a composition which takes deer heart, deer blood, bamboo leaves or bamboo leaf flavone, phytosterol, cordyceps militaris or an extract as main components, and the composition has the function of improving anoxia tolerance. The composition belongs to pure natural raw materials which can be used for food and health food, has definite components and no toxic or side effect, and has obvious function of improving anoxia endurance. Further experiments prove that the composition has better function of improving anoxia endurance compared with the single use of any one of the raw materials. The invention utilizes the synergistic effect of the components to prepare oral preparations, such as hard capsules, soft capsules, tablets, granules, powder, pills, soft sweets and medicinal liquor. The composition can be applied to food or health food, and has good effect in resisting anoxia.

Description

Deer heart blood composition helpful for improving anoxia tolerance
Technical Field
The invention relates to a composition taking deer heart freeze-dried powder, deer blood freeze-dried powder, phytosterol, bamboo leaf or bamboo leaf flavone and cordyceps militaris or an extract as main components. The composition has the function of improving anoxia tolerance, and the invention also relates to a medicinal preparation of the medicament, belonging to the technical field of medicines.
Background
Oxygen participates in metabolism of human body and is an indispensable substance for normal life activities. Under the resting state of an adult, the oxygen consumption per minute is about 250 ml; during activity, oxygen consumption increases. However, the oxygen storage in human body is very small, and the oxygen intake and transportation must be continuously completed by respiration and blood circulation depending on the supply of oxygen in the external environment to ensure the needs of cells. When the human tissue does not obtain sufficient oxygen or can not fully utilize the oxygen, the metabolism, the function and even the morphological structure of cells, tissues and organs can be abnormally changed, and the pathological process is called hypoxia. Hypoxia is an inferior stimulus to the body, affects various metabolisms of the body, particularly the oxidation function of the body, and finally leads to the death of the body due to insufficient supply of hypoxia to main organs such as the heart, the brain and the like. Oxygen and hypoxia are important topics of the basic theory of life sciences. The formation of hypoxia can be divided into three categories: the first is that the oxygen content in the external environment is reduced, so that enough oxygen cannot be taken in the normal physiological process, such as oxygen deficiency caused by special environments such as plateau, diving, aviation and the like; the second category is hypoxia caused by that the normal oxygen quantity outside can not reach the body sufficiently due to diseases and the like, so that the heart, the brain, the respiratory system and the like are caused; the third category is the oxygen consumption for body activities, which exceeds the physiological mobilization ability, resulting in insufficient relative oxygen supply, often seen in strenuous exercise and work overruns. The long-term hypoxia is an important hidden danger which endangers the health of human bodies, even serious people can endanger life, and the ventilation and/or ventilation dysfunction of the lung caused by lung diseases (pneumonia, pulmonary edema and the like), thoracic diseases (pleural effusion, pneumothorax and the like) and other diseases (such as hypothyroidism, metabolic alkalosis and the like) can occur, so that the research on the hypoxia-resistant medicine or the health-care food has wide market development prospect.
In recent years, with the traffic of the Qinghai-Tibet railway, the number of tourists entering the Tibet railway is continuously increased, and the acute altitude reaction becomes a great obstacle for the tourism industry. Meanwhile, with the continuous promotion of the economic construction of the Tibetan region, more and more people who enter the Tibetan work are more and more. In addition, a large number of army people live in high altitude areas all the year round, and an anti-hypoxia functional food is urgently needed to assist the army people to adapt to severe extreme climates in the plateau as soon as possible. Therefore, the development of high-quality and high-efficiency functional food with the function of improving the anoxia tolerance has very wide market prospect.
The invention relates to a natural medicine composition with an anti-hypoxia effect, which is found or extracted from natural plants. The composition is a natural product composition formed by innovatively combining effective components, embodies the characteristics of multiple targets and multiple effects of the traditional Chinese medicine, has the effect of treating both symptoms and root causes, and is expected to have remarkable breakthrough and improvement on curative effect and safety.
Since ancient times, deer blood is a precious product for treating diseases and building bodies of royal jelly and the sense organ is distinguished, and a compound product taking the deer blood as a main component is called 'Xianjia eating Danfang'. The water content of deer blood is 80% -81%, and the organic matter is 16% -17%, in which it is mainly protein, and the protein is rich in 19 amino acids and several enzymes, in addition, it also contains several lipids, free fatty acids, sterols, phospholipids, hormones, vitamins and polysaccharides, etc. And contains various major elements and beneficial trace elements. Especially deer blood also contains globulin, cystine, lysine, phosphocreatine kinase related to heart function, and covered enzyme. Promoting the regeneration of hemoglobin in human body and enhancing the hematopoietic function of human body. The deer blood taken by people in a proper amount can improve immunity, promote the regeneration of immune cells in human bodies, invigorate qi and blood, relieve physical weakness and enhance the antiviral capacity of human bodies, is particularly suitable for people with weak constitution and multiple diseases, and can obviously improve physical quality after being taken for a period of time. Improving the oxidation resistance of human body and delaying senility. The deer blood can prevent the generation of lipid peroxide in a human body and clear free radicals in the human body, and people can improve the functions of various organs of the human body and prevent various aging symptoms after frequent taking, and can delay aging and prolong life after frequent taking.
The deer heart is rich in tryptophan, lysine, histidine and other amino acids, fatty acid, total phosphoric acid, vitamins, trace elements and other nutrient substances and active ingredients required by human body. It is mainly used for treating dizziness, palpitation, and deficiency of both qi and blood. Can regulate myocardial function, remove blood stasis, relieve pain, enhance blood circulation, improve metabolism, nourish qi, tranquilize mind, has good curative effect on tachycardia, heart blood deficiency and other symptoms caused by overfatigue, fright and neurasthenia, angina pectoris, coronary heart disease, rheumatic heart disease and cerebral arteriosclerosis, and can effectively improve cardiopalmus, heartbeat, arrhythmia, insomnia, weakness, hypotension and the like. Modern researches show that deer heart has the functions of enhancing human metabolism and improving cardiovascular and cerebrovascular functions and nervous systems. Meanwhile, the peptide in the deer heart has the effects of regulating the dysfunction of the heart, promoting blood circulation, dredging orifices, promoting blood circulation and supplementing the motive power of cardiac muscle, thereby achieving the effect of maintaining the heart.
The bamboo leaf flavone is total flavone obtained by extracting Phyllostachys Pubescens (Phyllostachys meyeri) of Phyllostachys of Gramineae, concentrating, and purifying. The main ingredients are orientin, isoorientin, vitexin and isovitexin. Has the functions of resisting free radicals, resisting oxidation, resisting inflammation, enhancing immunity and delaying senility, and also has obvious functions of resisting anoxia, resisting fatigue and resisting stress. Systematic research (Chinese medicine new medicine and clinical pharmacology 2005, 16 (2): 100-; has obvious protective effect on acute ischemic and anoxic cardiac muscle, and the action mechanism is related to resisting free radical damage, enhancing the activity of antioxidant enzyme in vivo, reducing the oxygen consumption of cardiac muscle and reducing the area of myocardial infarction.
Phytosterols (phytosterol or plant sterol) are an active ingredient in plants and are structurally similar to animal sterols such as cholesterol. Is widely found in various vegetable oils, nuts and plant seeds, and also in other vegetable foods such as vegetables and fruits. Has the important functions of reducing the blood cholesterol content, inhibiting tumors, preventing and treating prostatic hyperplasia, inhibiting hyperplasia of mammary glands, regulating immunity and the like. Studies have shown that phytosterols significantly reduce the Total Cholesterol (TC) and Low Density Lipoprotein (LDL) levels in the blood, without reducing the High Density Lipoprotein (HDL) and triglyceride levels, resulting in a reduced LDL to HDL ratio without any significant side effects (Am J Med, 1999, 107 (6): 588-594). Blair et al also demonstrated that phytosterols have adjuvant therapeutic effects on hyperlipidemia in experiments (Am J Cardiol, 2000, 8 (6): 46-52); becker et al also demonstrated that sitosterol administration in children with severe familial hyperlipidemia significantly reduced blood lipid levels and had positive significance in reducing their adult cardiovascular and cerebrovascular diseases (J Pediatr, 1993, 122 (2): 292-; in a double-blind control study conducted by Sierksma et al on 76 healthy adults (average age 44 years), it was found that soy sterol administered at 0.8g/d for 3 weeks significantly reduced blood TC and LDL, but did not cause a reduction in blood levels of other fat-soluble vitamins such as carotene, as compared to the control group, and thus it is now widely accepted by scientists that the intake or supplementation of sufficient amounts of phytosterols helps to reduce the incidence of coronary heart disease in the population (Tidsskr Nor Laegeforen, 2000, 120 (22): 2661-2665).
Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, etc., is a complex composed of stroma (i.e., grass part) and sclerotium (i.e., dead body part of insect). Pharmacological effects modern science demonstrates that cordyceps militaris not only has special nutritional value, but also has obvious medicinal value. The traditional Chinese medicine considers that the cordyceps sinensis enters the lung and kidney two channels, can tonify lung yin and kidney yang, is mainly used for treating kidney deficiency, impotence and spermatorrhea, soreness and pain of waist and knees, weakness after illness, chronic cough and weakness, cough with phlegm and blood, spontaneous perspiration and night sweat and the like, and is the only traditional Chinese medicine capable of balancing and adjusting yin and yang simultaneously. Mainly contains various effective components including nucleosides, polysaccharides, sugar alcohols, sterols and SOD enzyme. Modern researches show that the cordyceps militaris has the effect of improving the memory of the brain; has effects in invigorating spleen, tranquilizing mind, and improving sleep quality; has effects in enhancing immunity and promoting hematopoiesis; has effects in improving respiratory system and enhancing pulmonary function; can regulate blood lipid in body; has antioxidant and anticancer activities.
The invention aims to provide a composition starting from 'multi-target and multi-effect' and having the function of improving anoxia tolerance.
The inventor of the invention discovers that various components in the formula have complementarity in the aspect of improving the anoxia tolerance according to different proportion formulas through a large number of screening tests, and unexpected effects are obtained after the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaves or bamboo leaf flavone, the phytosterol, the cordyceps militaris or the extract are combined.
Compared with single deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaf or bamboo leaf flavone, phytosterol, cordyceps militaris or extract, the composition has more obvious pharmacological activity. Therefore, the inventor of the invention carries out scientific and reasonable formula on each ingredient to obtain the composition which is beneficial to improving the anoxia endurance. The components of each ingredient start from different angles to achieve the function of improving anoxia endurance. Before the completion of the invention, no report exists that the composition consisting of the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaves or bamboo leaf flavone, the phytosterol, the cordyceps militaris or the extract is helpful for improving the anoxia endurance.
Disclosure of Invention
The inventor selects five raw material formulas which are specified by health management departments and can be used for food and new resource food from the basis of drug effect substances through repeated tests, such as deer heart, deer blood, bamboo leaves, phytosterol, cordyceps militaris and the like, so that the formula composition is very safe, and the health food or food is used for preparing health food or food which is helpful for improving anoxia tolerance and has no toxic or side effect. When the raw materials are selected for combination and matching, the effects of the raw materials are fully exerted, and a coordination effect is generated through reasonable matching. Besides the anti-ischemia and anti-anoxia substances, the invention also provides a more prominent contribution to the prevention and treatment of the emergency inflammatory exudation caused by ischemia and anoxia to the cardiovascular and cerebrovascular tissues. The bamboo leaf flavone with anti-inflammatory function is firstly mixed with deer heart, deer blood, phytosterol and cordyceps militaris which have the functions of anti-ischemia, anti-anoxia, anti-fatigue and immunity enhancement, and the composition is obviously different from western medicines with a single anti-anoxia and anti-ischemia target point in the market; meanwhile, compared with the existing Chinese patent medicine which is helpful for improving the anoxia endurance, the Chinese patent medicine has more definite activity and obvious characteristic of chemical composition, which is the outstanding contribution of the invention.
The deer heart has the functions of enhancing the metabolism of a human body, improving cardiovascular and cerebrovascular systems and nervous systems, promoting blood circulation and supplementing the motive power of cardiac muscle; deer blood has effects of promoting hemoglobin regeneration in human body, enhancing hematopoietic function of human body, enhancing immunity, and promoting immunocyte regeneration in human body; the bamboo leaf total flavonoids have the effects of scavenging free radicals, resisting oxidation, resisting inflammation, and protecting myocardial cells with anoxia and ischemia; the phytosterol has the function of regulating blood fat; cordyceps militaris has effects of improving immunity and hemopoiesis function, regulating blood lipid in vivo, and resisting oxidation. The chemical components act in a mutual cooperation way, multiple targets and multiple ways, thus fundamentally and comprehensively regulating the hypoxia tolerance of human bodies and improving the health condition of the human bodies. The invention achieves the aim of improving the anoxia endurance of the organism by the protection effect on the nervous system in the anoxia state, the protection effect on the cardiovascular system and the influence on the energy metabolism of the organism and by the improvement on the oxidation resistance of the organism and the resistance effect on anoxia-induced apoptosis. Has obvious innovation and technical progress.
The invention aims to provide a composition which is composed of deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaves or bamboo leaf flavone, phytosterol, cordyceps militaris or extracts and is used for contributing to the improvement of anoxia tolerance. Furthermore, the inventor discovers through further research that the composition formed by the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaf or bamboo leaf flavone, the phytosterol, the cordyceps militaris or the extract according to a formula in a certain proportion has a more remarkable effect in the aspect of improving the anoxia tolerance compared with the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaf or bamboo leaf flavone, the phytosterol, the cordyceps militaris or the extract. The natural medicine composition is obtained by the inventor through experimental screening for the first time, also embodies the synergistic effect of the compound traditional Chinese medicine, and has outstanding contribution and obvious technical progress.
The other outstanding contribution of the invention is that the edible lyophilized deer blood powder, lyophilized deer heart powder, bamboo leaf or bamboo leaf flavone, phytosterol, cordyceps militaris or extract are firstly combined into a composition which is firstly used for health-care food or food. The formula of the invention contains polysaccharide, flavone, vitamins, mineral substances and trace elements, and embodies the remarkable characteristics and technical progress of the formula. Meanwhile, the product has the advantages of simple preparation process, exact curative effect, no toxic or side effect, convenient taking and scientific dosage form, and embodies the practicability of the invention.
The invention provides a composition for improving anoxia endurance, which comprises 20-100 parts of deer heart freeze-dried powder, 40-150 parts of deer blood freeze-dried powder, 60-250 parts of bamboo leaves, 20-100 parts of phytosterol and 50-200 parts of cordyceps militaris. Or 10-40 parts of deer heart freeze-dried powder, 15-60 parts of deer blood freeze-dried powder, 20-80 parts of bamboo leaf flavone, 10-40 parts of phytosterol and 10-40 parts of cordyceps militaris extract. Which is prepared by mixing active ingredients and pharmaceutic adjuvants according to a proportion.
Another object of the present invention is to provide a method for preparing the composition:
a. preparing deer heart freeze-dried powder: freeze-drying Cor Cervi, and pulverizing to obtain Cor Cervi lyophilized powder;
b. preparing deer blood freeze-dried powder: freeze-drying sanguis Cervi, and pulverizing to obtain lyophilized sanguis Cervi powder;
c. preparing bamboo leaf flavone: extracting folium Bambusae with water or 20-50% ethanol under reflux for 1-2 hr for 2-3 times, filtering, and recovering solvent from the filtrate to obtain extract; passing the extract through pretreated AB-8 macroporous resin column, eluting with deionized water, discarding eluate, eluting with 10-25% ethanol, discarding eluate, eluting with 60-90% ethanol, collecting eluate, recovering ethanol, and drying to obtain total flavonoids of bamboo leaf;
d. preparing a cordyceps militaris extract: decocting Cordyceps militaris with 8-20 times of water for 2-3 times (1-3 hr each time), filtering, concentrating the filtrate, drying, and pulverizing to obtain Cordyceps militaris extract;
e. mixing the deer heart lyophilized powder, the deer blood lyophilized powder, the bamboo leaf flavone, the phytosterol and the cordyceps militaris extract according to a formula ratio, mixing with the pharmaceutical excipients, and preparing into an oral preparation according to a conventional method.
The invention discloses a composition comprising deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaves or bamboo leaf flavone, phytosterol and cordyceps militaris or extracts for the first time, and the composition is used for contributing to improving the function of anoxia tolerance. Therefore, the composition is prepared into a medicament independently or by being matched with other active components or auxiliary materials, and the medicament has the function of improving anoxia endurance, and belongs to the protection scope of the invention. The composition of the invention has the function of improving anoxia tolerance when being prepared into any dosage form, and is within the protection scope of the invention.
The composition (composition for short) consisting of the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaf or bamboo leaf flavone, the phytosterol and the cordyceps militaris or the extract has the function of improving the anoxia tolerance, and is proved by the following pharmacodynamic experiments.
Experiment raw materials: the composition and the single extract used in the pharmacodynamics are provided by the inventor.
The deer heart freeze-dried powder mentioned in the pharmacodynamic test is the sample prepared in example 1; the lyophilized deer blood powder is the sample prepared in example 2; bamboo leaf flavonoid was the sample prepared in example 3; cordyceps militaris extract was the sample prepared in example 4; phytosterols were purchased from seianche initiata biotechnology limited; the composition of the invention is the sample prepared in example 5 (deer heart lyophilized powder: deer blood lyophilized powder: bamboo leaf flavone: phytosterol: cordyceps militaris extract: 5: 8: 10: 5: 3).
The lactic acid determination kit is produced by Nanjing institute of bioengineering, and has a batch number of 20190102. The positive control drug, HONGJINGTIANSHENQI Capsule (batch No. 20191201, Sichuan Maili Oji scientific development Limited liability Co.).
160 ICR mice with the weight of 18-22 g are provided by the Changchun high New laboratory animal center, and the production license number of the laboratory animal is 2013-0010.
For each experiment, 100 mice were collected and divided into 10 groups of 10 mice each. Set up 8 test groups, 1 blank control group and 1 positive control group. The dosages of the test group of the composition are 100mg/kg, 200mg/kg and 400mg/kg respectively; the dosages of the deer blood freeze-dried powder, the deer heart freeze-dried powder, the bamboo leaf flavone, the phytosterol and the cordyceps militaris extract are all 400 mg/kg; distilled water was given to the blank control group; the dose of the positive control group is 300 mg/kg. Each group of experiment was gavaged 1 time per day for 30 consecutive days with a gavage volume of 0.1ml/10g body weight.
1. Effect of the composition on the constant pressure hypoxia-resistant survival time of mice
1h after the last sample administration, each group of mice was placed in 250ml ground bottles (1 per bottle) containing 5g of soda lime, capped and sealed, and the survival time of the mice was observed with apnea as an indication of death. The results are shown in Table 1.
TABLE 1 Effect of the compositions of the present invention on the survival time of mice to normbaric hypoxia
Figure BDA0002941869190000101
P < 0.05, P < 0.01, compared to the blank control group
As can be seen from Table 1, the low, medium and high dose groups of the composition of the present invention can prolong the survival time of mice, and the high and medium dose groups have significant difference compared with the blank control group in the survival time of hypoxia-resistant at normal pressure. And the high dose group mice survived longer than the low and medium dose group mice. Meanwhile, experimental data show that the high-dose composition has stronger pharmacological activity than single deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaf flavone, phytosterol and cordyceps militaris extract.
2. Effect of the composition of the invention on survival time of mouse sodium nitrite poisoning
1h after the last administration of the sample, animals of each group were intraperitoneally injected with sodium nitrite at a dose of 240mg/kg body weight, and the survival time of the animals was observed. The results are shown in Table 2.
TABLE 2 Effect of the compositions of the present invention on survival time of mice in sodium nitrite intoxication
Figure BDA0002941869190000111
P < 0.05, P < 0.01, compared to the blank control group
As shown in Table 2, 3 dose groups of the composition of the invention can obviously prolong the survival time of mice subjected to sodium nitrite poisoning compared with a blank control group, and the high and medium doses have significant difference. The high and medium dosage groups of the composition have stronger activity than single deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaf flavone, phytosterol and cordyceps militaris extract.
3. Effect of the composition of the present invention on survival time of acute cerebral ischemic hypoxia in mice
1h after the last sample administration, each group of animals broke their heads one by one from the neck, and immediately recorded the time from the head breaking of the mice to the stopping of the mouth breathing by a stopwatch. The results are shown in Table 3.
TABLE 3 Effect of the compositions of the present invention on survival time of acute cerebral ischemic hypoxia in mice
Figure BDA0002941869190000121
P < 0.05, P < 0.01, compared to the blank control group
As shown in Table 3, the high and medium dosage groups of the composition of the present invention can significantly prolong the survival time of acute cerebral ischemic anoxia of mice. Compared with single deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaf flavone, phytosterol and cordyceps militaris extract, the high-dose composition has stronger activity. 4. Effect of the composition of the present invention on blood lactic acid after exercise in mice
Blood is collected after the sample is given to the mouse for 30min in the last time, then the mouse swims in water with the temperature of 30 ℃ for 10min without load, then the mouse is taken out and blood is collected again immediately, and finally the 3 rd blood collection is carried out after the mouse has a rest for 20 min. The blood lactic acid content at 3 time points was determined according to the kit and the area under the lactic acid curve was calculated according to the following formula. The results are shown in Table 4.
Area under the blood lactic acid curve 5 × (blood lactic acid value before swimming +3 × blood lactic acid value after swimming +2 × blood lactic acid value after resting for 20min after swimming)
TABLE 4 Effect of the compositions of the present invention on blood lactate after exercise in mice
Figure BDA0002941869190000131
P < 0.05, P < 0.01, compared to the blank control group
As shown in Table 4, the high and medium dosage groups of the composition of the present invention can significantly reduce the area content under the blood lactic acid curve of mice after exercise. Thereby improving the energy supply level of the sugar metabolism system of the organism, reducing the proportion of protein metabolism energy supply, and improving the anti-anoxia capability. Meanwhile, test data show that the composition has stronger activity than single deer blood freeze-dried powder, deer heart freeze-dried powder, bamboo leaf flavone, phytosterol and cordyceps militaris extract.
Detailed Description
The following describes embodiments of the present invention in further detail with reference to examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 preparation method of Cor Cervi lyophilized powder
Cutting Cor Cervi into pieces, placing into tray of freeze dryer, freeze drying, and pulverizing to obtain sanguis Cervi lyophilized powder.
Example 2 preparation method of lyophilized deer blood powder
And (3) putting the deer blood into a tray of a freeze dryer, wherein the thickness is 8-10 mm, freeze-drying and crushing to obtain the deer blood freeze-dried powder.
Example 3 preparation method of bamboo leaf flavone
Extracting folium Bambusae with 40% ethanol under reflux for 3 times, each for 2 hr, filtering, and recovering solvent from filtrate to obtain ethanol extract; preparing the ethanol extract into a solution containing 1 g of crude drugs per ml, passing through a pretreated AB-8 macroporous resin column, eluting by using deionized water for 3 times of the column volume, discarding the eluent, eluting by using 15% ethanol for 3 times of the column volume, discarding the eluent, eluting by using 75% ethanol for 4 times of the column volume, collecting the eluent, recovering the ethanol, and drying to obtain the bamboo leaf flavone.
Example 4 preparation method of Cordyceps militaris extract
Decocting Cordyceps militaris with 12 times of water for 3 times (each time for 2 hr), filtering, concentrating the filtrate, drying, and pulverizing to obtain Cordyceps militaris extract.
EXAMPLE 5 preparation of the composition
Weighing 5g of deer heart freeze-dried powder, 8g of deer blood freeze-dried powder, 10g of bamboo leaf flavone, 5g of phytosterol and 3g of cordyceps militaris extract, and uniformly mixing to obtain the deer heart blood extract.
EXAMPLE 6 preparation of capsules
Weighing 50g of deer heart freeze-dried powder, 80g of deer blood freeze-dried powder, 100g of bamboo leaf flavone, 50g of phytosterol and 30g of cordyceps militaris extract, uniformly mixing, adding a proper amount of starch, uniformly mixing, and encapsulating to obtain the deer heart extract powder.
Example 7 preparation of tabletted confectioneries
Formulation of
Figure BDA0002941869190000151
Preparation method
Adding 200 ml of maltodextrin into water, boiling to completely dissolve, adding lyophilized powder of Cor Cervi and sanguis Cervi, stirring, mixing, drying under reduced pressure, and pulverizing; adding bamboo leaf flavone, phytosterol, Cordyceps militaris extract, starch, isomaltooligosaccharide and erythritol into the fine powder, mixing uniformly, granulating with 85% ethanol (containing citric acid and peppermint oil), drying at low temperature, finishing, tabletting, and making into 1000 tablets to obtain the tablet candy.
Example 8 preparation of fondant
Formulation of
Figure BDA0002941869190000152
Preparation method
Adding glucose, white granulated sugar, citric acid, sodium citrate and modified starch into 1 time of water, heating to completely dissolve, and sieving with 100 mesh sieve; adding gelatin into 8 times of water, heating to completely dissolve, mixing with the above sieved solution, adding Cor Cervi lyophilized powder, sanguis Cervi lyophilized powder, folium Bambusae flavone, phytosterol, and Cordyceps militaris extract, heating, decocting, cooling, adding 1:1 modified starch and peanut oil as release agent, coating release agent on a grinding tool, casting, drying at 40 deg.C for 1 hr, demolding, and packaging to obtain 1000 soft sweets.

Claims (8)

1. A composition useful for enhancing hypoxia tolerance, comprising: the active ingredients comprise lyophilized powder of Cor Cervi, lyophilized powder of sanguis Cervi, folium Bambusae or folium Bambusae flavone, phytosterol and Cordyceps militaris or extract.
2. The composition for improving anoxia endurance as claimed in claim 1, wherein it is prepared from the following raw materials in parts by weight: 20-100 parts of deer heart freeze-dried powder, 40-150 parts of deer blood freeze-dried powder, 60-250 parts of bamboo leaves, 20-100 parts of phytosterol and 50-200 parts of cordyceps militaris.
3. The composition for improving anoxia endurance of claim 1, which is prepared from the following raw materials in parts by weight: 10-40 parts of deer heart freeze-dried powder, 15-60 parts of deer blood freeze-dried powder, 20-80 parts of bamboo leaf flavone, 10-40 parts of phytosterol and 10-40 parts of cordyceps militaris extract.
4. A method of preparing a composition for use in improving hypoxia tolerance according to claim 3, comprising the steps of:
a. preparing deer heart freeze-dried powder: freeze-drying Cor Cervi, and pulverizing to obtain Cor Cervi lyophilized powder;
b. preparing deer blood freeze-dried powder: freeze-drying sanguis Cervi, and pulverizing to obtain lyophilized sanguis Cervi powder;
c. preparing bamboo leaf flavone: extracting folium Bambusae with water or 20-50% ethanol under reflux for 1-2 hr for 2-3 times, filtering, and recovering solvent from the filtrate to obtain extract; passing the extract through pretreated AB-8 macroporous resin column, eluting with deionized water, discarding eluate, eluting with 10-25% ethanol, discarding eluate, eluting with 60-90% ethanol, collecting eluate, recovering ethanol, and drying to obtain total flavonoids of bamboo leaf;
d. preparing a cordyceps militaris extract: decocting Cordyceps militaris with 8-20 times of water for 2-3 times (1-3 hr each time), filtering, concentrating the filtrate, drying, and pulverizing to obtain Cordyceps militaris extract;
e. mixing the deer heart lyophilized powder, the deer blood lyophilized powder, the bamboo leaf flavone, the phytosterol and the cordyceps militaris extract according to a formula ratio, mixing with the pharmaceutical excipients, and preparing into an oral preparation according to a conventional method.
5. A pharmaceutical formulation characterized by: comprising the composition of claims 1-4 in a therapeutically effective amount in combination with one or more pharmaceutically acceptable excipients.
6. The pharmaceutical preparation according to claim 5, wherein the preparation is selected from one or more of hard capsules, soft capsules, tablets, granules, powders, pills, soft candies, medicated liquors and oral liquids.
7. The pharmaceutical excipient according to claim 5 comprising a filler selected from the group consisting of starch, isomaltooligosaccharide, microcrystalline cellulose, maltodextrin, etc.; the disintegrating agent is selected from sodium carboxymethyl starch, hydroxypropyl cellulose, and crospovidone; the flavoring agent is selected from citric acid, peppermint oil, erythritol, etc.; optionally, a binder, wetting agent and lubricant.
8. Use of the composition according to claims 1-7 for improving hypoxia tolerance in the preparation of anti-hypoxia drugs.
CN202110182738.4A 2021-02-07 2021-02-07 Deer heart blood composition helpful for improving anoxia tolerance Pending CN112891473A (en)

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Citations (3)

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Publication number Priority date Publication date Assignee Title
CN103734711A (en) * 2013-11-24 2014-04-23 郭国康 Composition for promoting heart health
CN105878803A (en) * 2016-05-12 2016-08-24 赵全成 Ginseng and bamboo leaf composition capable of preventing and treating alcoholic liver injuries
CN111388511A (en) * 2020-04-13 2020-07-10 杜锐 Composition for improving memory and preventing senile dementia and preparation method and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103734711A (en) * 2013-11-24 2014-04-23 郭国康 Composition for promoting heart health
CN105878803A (en) * 2016-05-12 2016-08-24 赵全成 Ginseng and bamboo leaf composition capable of preventing and treating alcoholic liver injuries
CN111388511A (en) * 2020-04-13 2020-07-10 杜锐 Composition for improving memory and preventing senile dementia and preparation method and application thereof

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Application publication date: 20210604