CN112890198A - 一种利用大蒜生产合生元的方法及其应用 - Google Patents
一种利用大蒜生产合生元的方法及其应用 Download PDFInfo
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Abstract
本发明公开了一种利用大蒜生产合生元的方法及其应用,属于农产品深加工技术领域。本发明将鲜大蒜经预处理、加热灭活蒜氨酸酶、打浆制汁、加果聚糖水解酶水解获得低聚果糖溶液、再经杀菌、培养益生菌、干燥等工序而制得的既含有低聚果糖益生元、又含有活性益生菌的无蒜臭味合生元产品。本发明所获合生元保留了大蒜中原有保健成分,不使用冻结保护剂,降低了生产成本。
Description
技术领域
本发明属于农产品深加工技术领域,具体涉及一种利用大蒜生产合生元的方法及其应用。
背景技术
根据微生态理论,绿色环保的微生物可以调整人体肠道微生态失衡、改善肠道菌群,进而具有养生、保健、治疗等保健功能;具有该功能的益生菌、益生元(可作为益生菌的碳源而促进其生长)、合生元保健食品,已经逐渐被大多数人所接受,其产品需求越来越多。
大蒜富含果聚糖、低聚果糖,并可作为益生元促进使乳酸菌(干酪乳杆菌、嗜酸乳杆菌、植物乳杆菌、解淀粉芽孢杆菌)等益生菌的生长,发酵产生短链脂肪酸,显示出较强的改善肠道菌群的保健功能。
大蒜果聚糖(一般含低聚果糖约30%)改善肠道菌群功能,与其聚合度密切相关:其聚合度越低、改善肠道微生态的功能越强,如益生菌的蔗果三糖利用率≥60%,高于蔗果四糖的利用率≥38%。
另据报道:大蒜果聚糖可以使急性酒精性肝损伤小鼠的肠道菌群多样性和均匀度指数增加,优杆菌属和乳酸菌属细菌数量增多,对急性酒精性肝损伤伴随的肠道菌群失调具有调节作用;还有报道表明大蒜果聚糖可以使酒精性肝纤维化小鼠的肠道菌群丰富度增加,使毛螺菌属和乳酸杆菌属增多,费克蓝姆氏菌属和厚壁菌门减少,可用于治疗酒精性肝纤维化小鼠的肠道菌群失调。
由此可见,大蒜低聚果糖具有增殖益生菌、改善肠道菌群的作用。
但由于大蒜具有明显的蒜臭味,难以为大多数所接受;且其所含的果聚糖的聚合度较高(其聚合度一般在30-50),调节肠道菌群功能比聚合度低的低聚果糖小得多。因此,有必要开发一种既含有低聚果糖又含有益生菌的无蒜臭味的合生元类产品。
发明内容
针对以上现有技术存在的缺点和不足之处,本发明的首要目的在于提供一种利用大蒜生产合生元的方法。本发明以大蒜为原料,通过本发明所述的方法生产的合生元产品既含有低聚果糖又含有益生菌,而且无蒜臭味。
本发明的另一个目的在于提供上述利用大蒜生产合生元的方法的应用。
本发明的目的通过下述技术方案实现:
一种利用大蒜生产合生元的方法,包括如下步骤:
(1)大蒜原料的预处理:去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣用水或水蒸汽加热1~15分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将水与灭活蒜氨酸酶的蒜瓣按照体积比1~3:1的比例,打浆,经压榨、取上清即得清亮蒜汁;
(4)果聚糖水解:向步骤(3)得到的清亮蒜汁中加入果聚糖水解酶,于室温下水解1~4h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为10%~15%时,将其加热至100℃保温10~15min,冷却,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中接种益生菌,恒温培养18~20h,每隔3~4h搅拌一次,使其发酵至乳酸含量1%~1.5%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液干燥,过筛,即得合生元产品。
步骤(1)中所述的可食蒜瓣优选为符合卫生要求的可食大蒜。
步骤(2)中所述的加热的时间优选为5~15分钟。
步骤(3)中所述的水优选为煮沸水。
步骤(3)中所述的取上清的方法优选为通过离心或过滤实现。
所述的果聚糖水解酶是指商业果聚糖水解酶和自制果聚糖水解酶中的至少一种;所述的自制果聚糖水解酶是指粗果聚糖水解酶,即菊粉酶粗酶液,所述的粗果聚糖水解酶按照李晓丹,李秉超.黑曲霉固体发酵产菊粉酶的研究[J].中国酿造,2008(5):54-56中记载的方法制备。
所述的商业果聚糖水解酶的酶活力优选为0.5×105~1.5×105UI/g;更优选为105UI/g。
所述的粗果聚糖水解酶的酶活力为0.5×104~1.5×104UI/g;更优选为104UI/g。
所述的商业果聚糖水解酶的用量优选为清亮蒜汁质量的0.01%~0.02%。
所述的粗果聚糖水解酶的用量优选为大蒜原料质量的0.08%~0.12%。
步骤(3)中所述的清亮蒜汁中的可溶性固形物含量优选为8%~12%;更优选为8%。
步骤(4)中所述的室温是指25~40℃。
步骤(4)中所述的水解的时间优选为2h。
步骤(4)中所述的冷却优选为冷却至40℃。
步骤(5)中所述的果糖还原值是指果糖占低聚果糖的比例。
步骤(6)中所述的益生菌优选包括保加利亚乳杆菌;更优选为嗜酸乳杆菌、双歧杆菌、植物乳杆菌和罗伊氏乳杆菌中的至少一种,以及保加利亚乳杆菌;最优选为嗜酸乳杆菌、双歧杆菌、植物乳杆菌、罗伊氏乳杆菌和保加利亚乳杆菌。
所述的嗜酸乳杆菌的活菌数优选为1.0×1011~3.0×1011个/mL。
所述的双歧杆菌的活菌数优选为1.0×1011~3.0×1011个/mL。
所述的植物乳杆菌的活菌数优选为1.0×1010~3.0×1010个/mL。
所述的罗伊氏乳杆菌的活菌数优选为1.0×1011~3.0×1011个/mL。
所述的保加利亚乳杆菌的活菌数优选为1.0×1010~3.0×1010个/mL。
当所述的益生菌为嗜酸乳杆菌、双歧杆菌、植物乳杆菌、罗伊氏乳杆菌和保加利亚乳杆菌时,所述的嗜酸乳杆菌、双歧杆菌、植物乳杆菌、罗伊氏乳杆菌和保加利亚乳杆菌的体积比优选为0.78~1.46:0.78~1.46:0.78~1.46:0.78~1.46:2~3.25;
步骤(6)中所述的益生菌的总接种量优选按清亮蒜汁体积的3%~5%计算。
步骤(6)中所述的恒温培养的温度优选为38~42℃;更优选为40℃。
步骤(6)中所述的搅拌的转速优选为55~65r/min,搅拌的时间为1~3min;更优选搅拌的转速为60r/min,搅拌的时间为2min。
步骤(7)中所述的干燥还包括向益生菌发酵液中加入脱脂奶粉、麦芽糖和乳糖的操作;所述的益生菌发酵液的体积(mL)与脱脂奶粉、麦芽糖和乳糖的质量(mg)比优选为:9.5~10.5:4.75~5.25:0.95~1.05:0.95~1.05;更优选按照10:5:1:1计算;
当益生菌发酵液中含有脱脂奶粉、麦芽糖和乳糖时,所述的冷冻干燥的方法优选为:将益生菌发酵液、脱脂奶粉、麦芽糖和乳糖按照益生菌发酵液的体积(mL)与脱脂奶粉、麦芽糖和乳糖的质量(mg)比为:9.5~10.5:4.75~5.25:0.95~1.05:0.95~1.05混匀、溶解后,经喷雾干燥进行干燥。
当益生菌发酵液中不含有脱脂奶粉、麦芽糖和乳糖时,所述的干燥优选为冷冻干燥;所述的冷冻干燥的方法优选为:将益生菌发酵液以3000r/min的转速离心15~20min制成益生菌菌泥后,将其在-40~-20℃预冻24h至菌泥完全冻结后,再移至冷冻干燥机冷冻干燥。
步骤(7)中所述的过筛优选为过40~80目筛。
上述利用大蒜生产合生元的方法在生产改善肠道菌群的保健食品中的应用。
与现有技术相比,本发明存在如下优点及有益效果:
(1)本发明将鲜大蒜经预处理、加热灭活蒜氨酸酶、打浆制汁、加果聚糖水解酶水解获得低聚果糖溶液、再经杀菌、培养益生菌、干燥等工序而制得的既含有低聚果糖益生元、又含有活性益生菌的无蒜臭味合生元产品。
大蒜原料易得,还可以充分利用不适食用鲜销的小蒜、收获时受伤蒜等残次蒜,或生产大蒜精油后的蒜渣等原料进行生产;
(2)通过加热使蒜氨酸酶失活,从而较好地去除了大蒜的蒜臭味;
(3)通过水解果聚糖所产生的果糖、较少量的葡萄糖,满足了益生菌发酵所需要的糖源,免除了大部分益生元产品含葡萄糖多而导致的血糖升高风险;
(4)本发明所获产品不仅含有含硫氨基酸、酚酸、活性硫、多糖等具有保健作用的生物活性成分、即较好地保存了大蒜原有的营养成分,而且还具有便于贮存、使用方便等优点,能用于生产冲剂、压片、固体饮料等类型的食品和保健食品;
(5)本发明制备的合生元产品含有大量的低聚果糖、果聚糖等多糖、植物胶等保护益生菌活力的天然保护剂,因而在冻结、干燥过程中可以大大节省抗冻结、干燥所造成的菌体失活损失和生产原料成本等优点。
(6)本发明所获合生元保留了大蒜中原有保健成分,不使用冻结保护剂,降低了生产成本。
附图说明
图1为实施例1利用大蒜生产合生元的方法的制备流程图。
具体实施方式
下面结合实施例对本发明作进一步详细的描述,但本发明的实施方式不限于此。
实施例1:
一种利用大蒜生产合生元的方法,简要的工艺流程图请参见图1,包括如下步骤:
(1)大蒜原料的预处理:称取大蒜蒜瓣原料100kg,去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣置于沸水中加热5分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将煮沸水与灭活蒜氨酸酶的蒜瓣按照体积比1:1的比例,一起加入打浆机中破碎成浆,经压榨、离心取上清即得清亮蒜汁约180kg(调整可溶性固形物含量为8%,实际工业生产中,1kg=1L;下同);
(4)果聚糖水解:趁温(约40℃)向步骤(3)得到的清亮蒜汁中加入粗果聚糖水解酶(制备方法详见:李晓丹,李秉超.黑曲霉固体发酵产菊粉酶的研究[J].中国酿造,2008(5):54-56.酶活力为104UI/g 0.1kg(以大蒜原料质量的0.1%计),于室温(25℃)下水解2h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为10%时,将其加热至100℃保温10min,冷却至40℃,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中分别接种0.78L的嗜酸乳杆菌(活菌数为2.0×1011个/mL)、双歧杆菌(活菌数为1.0×1011个/mL)、植物乳杆菌(活菌数为1.0×1010个/mL)、罗伊氏乳杆菌(3.0×1011个/mL)和2.34L的保加利亚乳杆菌(2.0×1010个/mL),在40℃恒温培养18h,每隔3~4h搅拌一次,转速60转/min,搅拌时间2min,使其发酵至乳酸含量1.5%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液、脱脂奶粉、麦芽糖、乳糖按照益生菌发酵液的体积(mL)与脱脂奶粉、麦芽糖和乳糖的质量(mg)比为:10:5:1:1的比例混匀、溶解后,经喷雾干燥冷却到室温后过60目筛,得合生元产品。
性能测试
对实施例1得到的合生元产品的乳酸菌数、果糖还原值和乳酸含量进行测定,检测方法如下:
乳酸菌数的检测参考GB4789.35-2016食品安全国家标准食品微生物学检验乳酸菌检验,GB/T34224-2017《生物产品中功能性微生物检测》进行测定;
果糖还原值的测定参考GB_20885_2007葡萄糖浆中DE值进行测定;
乳酸含量的测定参考GB 1886.173-2016食品安全国家标准食品添加剂乳酸。
检测结果如下表1所示:
表1:实施例1得到的合生元产品的部分质量指标
表1的结果说明,所获产品既含有大量的有益的乳酸菌,又含有具有促进乳酸菌类益生菌生长的低聚糖果糖成分(其果糖还原值高于步骤(5)中水解产物的果糖还原值,是因为在加工过程中,还有一定量的果聚糖发生了水解所致),因而本实施例所获产品,可以作为改善肠道菌群的合生元类产品。
实施例2:
一种利用大蒜生产合生元的方法,包括如下步骤:
(1)大蒜原料的预处理:称取大蒜蒜瓣原料50kg,去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣置于沸水中加热15分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将煮沸水与灭活蒜氨酸酶的蒜瓣按照体积比2:1的比例,一起加入打浆机中破碎成浆,经压榨、离心获得清亮蒜汁约145kg(调整可溶性固形物含量为8%);
(4)果聚糖水解:趁温(约40℃)向步骤(3)得到的清亮蒜汁中加入果聚糖水解酶(购于山东福旺嘉生物科技有限公司,酶活力为105UI/g)29g,于室温(35℃)下水解2h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为12%时,将其加热至100℃保温15min后,冷却至40℃,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中分别接种1L的嗜酸乳杆菌(活菌数为1.0×1011个/mL)、双歧杆菌(活菌数为2.0×1011个/mL)、植物乳杆菌(活菌数为3.0×1010个/mL)、罗伊氏乳杆菌(2.0×1011个/mL)和3.25L的保加利亚乳杆菌(1.0×1010个/mL),在40℃恒温培养19h,每隔3~4h搅拌一次,转速60转/min,搅拌时间2min,使其发酵至乳酸含量1.05%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液以3000r/min的转速离心20min后制成益生菌菌泥,取菌泥在-20℃预冻24h至其完全冻结后,再移至冷冻干燥机冷冻干燥,即可获得冻干合生元的干粉产品。
实施例3:
一种利用大蒜生产合生元的方法,包括如下步骤:
(1)大蒜原料的预处理:称取大蒜蒜瓣原料100kg,去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣置于沸水中加热5分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将煮沸水与灭活蒜氨酸酶的蒜瓣按照体积比3:1的比例,一起加入打浆机中破碎成浆,经压榨、离心取上清即得清亮蒜汁约220kg(调整可溶性固形物含量为8%);
(4)果聚糖水解:趁温(约40℃)向步骤(3)得到的清亮蒜汁中加入果聚糖水解酶(来源及酶活同实施例2)22g,于室温下水解2h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为15%时,将其加热至100℃保温13min,冷却至40℃,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中分别接种1.46L的嗜酸乳杆菌(活菌数为3.0×1011个/mL)、双歧杆菌(活菌数为3.0×1011个/mL)、植物乳杆菌(活菌数为2.0×1010个/mL)、罗伊氏乳杆菌(1.0×1011个/mL)和2.92L的保加利亚乳杆菌(3.0×1010个/mL),在40℃恒温培养20h,每隔3~4h搅拌一次,转速60转/min,搅拌时间2min,使其发酵至乳酸含量1.3%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液、脱脂奶粉、麦芽糖、乳糖按照益生菌发酵液的体积(mL)与脱脂奶粉、麦芽糖和乳糖的质量(mg)比为:10:5:1:1的比例混匀、溶解后,经喷雾干燥冷却到室温后过40目筛,得合生元产品。
实施例4:
一种利用大蒜生产合生元的方法,包括如下步骤:
(1)大蒜原料的预处理:称取不适于鲜销大蒜(小头蒜、有机械伤蒜、形态不规整蒜等残次蒜)蒜瓣原料100kg,去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣置于蒸笼上,加热圆汽5分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将无菌水与灭活蒜氨酸酶的蒜瓣按照体积比1.5:1的比例,一起加入打浆机中破碎成浆,经压榨、离心获得清亮蒜汁约200kg(调整可溶性固形物含量为8%);
(4)果聚糖水解:趁温(约40℃)向步骤(3)得到的清亮蒜汁中加入果聚糖水解酶(来源及酶活同实施例2)30g,于室温下水解2h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为15%时,将其加热至100℃保温13min,冷却至40℃,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中分别接种1L的嗜酸乳杆菌(活菌数为3.0×1011个/mL)、双歧杆菌(活菌数为3.0×1011个/mL)、植物乳杆菌(活菌数为2.0×1010个/mL)、罗伊氏乳杆菌(1.0×1011个/mL)和2L的保加利亚乳杆菌(3.0×1010个/mL),在40℃恒温培养20h,每隔3~4h搅拌一次,转速60转/min,搅拌时间2min,使其发酵至乳酸含量1.3%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液、脱脂奶粉、麦芽糖、乳糖按照益生菌发酵液的体积(mL)与脱脂奶粉、麦芽糖和乳糖的质量(mg)比为:10:5:1:1的比例混匀、溶解后,经喷雾干燥冷却到室温后过40目筛,得合生元产品。
上述实施例为本发明较佳的实施方式,但本发明的实施方式并不受上述实施例的限制,其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化,均应为等效的置换方式,都包含在本发明的保护范围之内。
Claims (10)
1.一种利用大蒜生产合生元的方法,其特征在于,包括如下步骤:
(1)大蒜原料的预处理:去除大蒜原料的泥土、皮、蒂、苔梗后,饮用水洗涤干净,得到可食蒜瓣;
(2)灭活蒜氨酸酶:将步骤(1)中的可食蒜瓣用水或水蒸汽加热1~15分钟,得到灭活蒜氨酸酶的蒜瓣;
(3)打浆取汁:将水与灭活蒜氨酸酶的蒜瓣按照体积比1~3:1的比例,打浆,经压榨、取上清即得清亮蒜汁;
(4)果聚糖水解:向步骤(3)得到的清亮蒜汁中加入果聚糖水解酶,于室温下水解1~4h,得到水解产物;
(5)杀菌:检测步骤(4)得到的水解产物的果糖还原值为10%~15%时,将其加热至100℃保温10~15min,冷却,即为大蒜果聚糖水解液;
(6)培养益生菌:向步骤(5)的大蒜果聚糖水解液中接种益生菌,恒温培养18~20h,每隔3~4h搅拌一次,使其发酵至乳酸含量1%~1.5%(w/v)终止发酵,得到益生菌发酵液;
(7)干燥:将步骤(6)中的益生菌发酵液干燥,过筛,即得合生元产品。
2.根据权利要求1所述的方法,其特征在于,
步骤(2)中所述的加热的时间为5~15分钟。
3.根据权利要求1所述的方法,其特征在于,
所述的果聚糖水解酶是指商业果聚糖水解酶和自制果聚糖水解酶中的至少一种;
所述的自制果聚糖水解酶是指粗果聚糖水解酶,即菊粉酶粗酶液,所述的粗果聚糖水解酶按照李晓丹,李秉超.黑曲霉固体发酵产菊粉酶的研究[J].中国酿造,2008(5):54-56中记载的方法制备。
4.根据权利要求3所述的方法,其特征在于,
所述的商业果聚糖水解酶的酶活力为0.5×105~1.5×105UI/g;
所述的粗果聚糖水解酶的酶活力为0.5×104~1.5×104UI/g。
5.根据权利要求4所述的方法,其特征在于,
所述的商业果聚糖水解酶的用量为清亮蒜汁质量的0.01%~0.02%;
所述的粗果聚糖水解酶的用量为大蒜原料质量的0.08%~0.12%。
6.根据权利要求1所述的方法,其特征在于,
步骤(6)中所述的益生菌包括保加利亚乳杆菌;进一步为嗜酸乳杆菌、双歧杆菌、植物乳杆菌和罗伊氏乳杆菌中的至少一种,以及保加利亚乳杆菌;
所述的嗜酸乳杆菌的活菌数为1.0×1011~3.0×1011个/mL;
所述的双歧杆菌的活菌数为1.0×1011~3.0×1011个/mL;
所述的植物乳杆菌的活菌数为1.0×1010~3.0×1010个/mL;
所述的罗伊氏乳杆菌的活菌数为1.0×1011~3.0×1011个/mL;
所述的保加利亚乳杆菌的活菌数为1.0×1010~3.0×1010个/mL;
当所述的益生菌为嗜酸乳杆菌、双歧杆菌、植物乳杆菌、罗伊氏乳杆菌和保加利亚乳杆菌时,所述的嗜酸乳杆菌、双歧杆菌、植物乳杆菌、罗伊氏乳杆菌和保加利亚乳杆菌的体积比为0.78~1.46:0.78~1.46:0.78~1.46:0.78~1.46:2~3.25;
步骤(6)中所述的益生菌的总接种量按清亮蒜汁体积的3%~5%计算。
7.根据权利要求1所述的方法,其特征在于,步骤(7)中所述的干燥还包括向益生菌发酵液中加入脱脂奶粉、麦芽糖和乳糖的操作;所述的益生菌发酵液的体积mL与脱脂奶粉、麦芽糖和乳糖的质量mg比为:9.5~10.5:4.75~5.25:0.95~1.05:0.95~1.05;
当益生菌发酵液中含有脱脂奶粉、麦芽糖和乳糖时,所述的冷冻干燥的方法为:将益生菌发酵液、脱脂奶粉、麦芽糖和乳糖按照益生菌发酵液的体积mL与脱脂奶粉、麦芽糖和乳糖的质量mg比为:9.5~10.5:4.75~5.25:0.95~1.05:0.95~1.05混匀、溶解后,经喷雾干燥进行干燥;
当益生菌发酵液中不含有脱脂奶粉、麦芽糖和乳糖时,所述的干燥为冷冻干燥;所述的冷冻干燥的方法为:将益生菌发酵液以3000r/min的转速离心15~20min制成益生菌菌泥后,将其在-40~-20℃预冻24h至菌泥完全冻结后,再移至冷冻干燥机冷冻干燥。
8.根据权利要求1所述的方法,其特征在于,
步骤(1)中所述的可食蒜瓣为符合卫生要求的可食大蒜;
步骤(3)中所述的水为煮沸水;
步骤(3)中所述的取上清的方法为通过离心或过滤实现;
步骤(3)中所述的清亮蒜汁中的可溶性固形物含量为8%~12%;
步骤(4)中所述的室温是指25~40℃;
步骤(4)中所述的水解的时间为2h。
9.根据权利要求1所述的方法,其特征在于,
步骤(6)中所述的恒温培养的温度为38~42℃;
步骤(6)中所述的搅拌的转速为55~65r/min,搅拌的时间为1~3min;
步骤(7)中所述的过筛为过40~80目筛。
10.权利要求1~9任一所述的利用大蒜生产合生元的方法在生产改善肠道菌群的保健食品中的应用。
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