CN112868607A - Method for controlling bad pupa disease of Chouioia cunea Yang breeding - Google Patents
Method for controlling bad pupa disease of Chouioia cunea Yang breeding Download PDFInfo
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- CN112868607A CN112868607A CN202110222607.4A CN202110222607A CN112868607A CN 112868607 A CN112868607 A CN 112868607A CN 202110222607 A CN202110222607 A CN 202110222607A CN 112868607 A CN112868607 A CN 112868607A
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- 241000922327 Chouioia cunea Species 0.000 title claims abstract description 99
- 238000009395 breeding Methods 0.000 title claims abstract description 76
- 230000001488 breeding effect Effects 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000382353 Pupa Species 0.000 title claims abstract description 29
- 201000010099 disease Diseases 0.000 title claims abstract description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 17
- 230000001954 sterilising effect Effects 0.000 claims abstract description 43
- 238000011081 inoculation Methods 0.000 claims abstract description 16
- 241000255789 Bombyx mori Species 0.000 claims abstract description 8
- 230000000249 desinfective effect Effects 0.000 claims abstract description 7
- 238000010792 warming Methods 0.000 claims description 10
- 241001411320 Eriogonum inflatum Species 0.000 claims description 9
- 239000000645 desinfectant Substances 0.000 claims description 9
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000002390 adhesive tape Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 235000013601 eggs Nutrition 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000002023 wood Substances 0.000 claims description 3
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 2
- 240000001624 Espostoa lanata Species 0.000 claims description 2
- 241000257303 Hymenoptera Species 0.000 claims description 2
- 238000010009 beating Methods 0.000 claims description 2
- 241001641310 Cunea Species 0.000 claims 1
- 230000002070 germicidal effect Effects 0.000 claims 1
- 230000017074 necrotic cell death Effects 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 210000001015 abdomen Anatomy 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 230000029264 phototaxis Effects 0.000 abstract description 3
- 230000032669 eclosion Effects 0.000 description 4
- 241001531327 Hyphantria cunea Species 0.000 description 3
- 241000256846 Apis cerana Species 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 241000484025 Cuniculus Species 0.000 description 2
- 241000248484 Ichthyophthirius Species 0.000 description 2
- 241000256856 Vespidae Species 0.000 description 2
- 238000000889 atomisation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 241000922323 Chouioia Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
A method for controlling bad pupa disease of Chouioia cunea Yang breeding relates to the technical field of pest control, in particular to a method for breeding Chouioia cunea Yang. According to a bad pupa disease propagation mechanism, the main link of the Chouioia cunea Yang inoculation is sterilized and disinfected, so that the propagation of bad pupa pathogenic bacteria in the bee inoculation process is effectively inhibited; the use of the net-covered type Chouioia cunea Yang breeding box with the gauze at the top makes full use of the phototaxis of the Chouioia cunea Yang to ensure that the abdomen of the Chouioia cunea Yang is upward laid on the gauze, so that the ovipositor of the Chouioia cunea Yang can be sterilized and disinfected by disinfector and ultraviolet rays more conveniently, and the sterilizing and disinfecting effects are improved; the breeding box is communicated with the inside and the outside, which is beneficial to controlling the humidity in the box and creating the environment which is not beneficial to the silkworm chrysalis to rot. The method can improve the breeding efficiency, reduce the economic loss and reduce the pupa breeding rate by more than 30 percent, thereby achieving the aim of stably providing the natural enemy insect Chouioia cunea Yang, and making more contribution to controlling insects by insects and protecting the ecological environment.
Description
Technical Field
The invention relates to the technical field of pest control, in particular to a breeding method for Chouioia cunea Yang.
Background
Chouioia cunea Yang is an important parasitic natural enemy of lepidoptera leaf-eating pests such as fall webworms. The breeding of Chouioia cunea Yang by using the intermediate tussah pupa is a mature technology. The national forestry agency releases ' artificial breeding and application technical regulation ' of Chouioia cunea Yang ' in 2007 in 6 and 4 months, according to the regulation and the commonly applied technology, a large amount of tussah pupas are rotten in the breeding process, sometimes the rate of the rotten pupas is as high as more than 60%, the breeding efficiency is seriously influenced, and great loss is caused to the breeding work; against the above problems, there has been no practical and robust solution; the inventor finds that the key measure for reducing the rate of the bad pupae is to disinfect adult Chouioia cunea Yang and an ovipositor thereof in the breeding and inoculating process.
Disclosure of Invention
The invention provides a method for controlling bad pupa disease of breeding Chouioia cunea Yang, which is used for sterilizing female Chouioia cunea Yang and an ovipositor thereof so as to reduce the bad pupa rate.
The invention provides a method for controlling bad pupa disease of breeding Chouioia cunea Yang, which is characterized by comprising the following steps:
the method comprises the following steps: putting the breeding pupae into a bee warming bottle, covering the bottle stopper, and waiting for the Chouioia cunea Yang to emerge from the breeding pupae;
step two: opening the bottle stopper to sterilize the emerged Chouioia cunea Yang in the thermos bottle;
step three: placing the box body of the net-covered type Chouioia cunea Yang breeding box with the silkworm chrysalis on an inoculation workbench;
step four: opening a sterilizing lamp above an inoculation workbench, beating a warm bee bottle under the irradiation of the sterilizing lamp to uniformly spread the eclosion ichthyophthirius cuniculus in the warm bee bottle into a box body of a net-covered type ichthyophthirius cuniculus breeding box for inoculation, and covering a box cover with a gauze net at the top;
step five: sterilizing and disinfecting the newly-inoculated Chouioia cunea Yang in the net-covered Chouioia cunea breeding box;
step six: transferring the net-covered type Chouioia cunea Yang breeding box to a Chouioia cunea frame for sterilizing by irradiation of a sterilizing lamp;
step seven: turning off the sterilizing lamp, and keeping the dark light to enable the breeding bees to lay eggs in the silkworm pupa body;
step eight: and turning on a sterilizing lamp for 15 minutes every day, tearing off the adhesive tape after 1-2 days, taking off the box cover, opening the net-covered type Chouioia cunea Yang breeding box, and turning to a temperature and humidity control culture stage.
Further, the thermos bottle is characterized in that the thermos bottle is a transparent plastic bottle, and the bottle stopper is a cotton ball.
Further, the method is characterized in that the mode of sterilizing the eclosion webworm Chouioia in the bee-warming bottle is spray sterilization; after diluting the disinfectant, rapidly spraying the disinfectant at a position 1 m away from the mouth of the wasp bottle by using an atomizer.
Further, the method is characterized in that the next inoculation step is carried out after the eclosion of Chouioia cunea Yang in the bee-warming bottle is sterilized and disinfected for 30 minutes.
Further, the method is characterized in that the mode of sterilizing and disinfecting the newly-inoculated Chouioia cunea Yang in the net-covered Chouioia cunea Yang breeding box is spray sterilization and disinfection; under the irradiation of a sterilizing lamp, the diluted disinfectant is quickly sprayed by an atomizer at a distance of 1 m from a gauze on the top surface of the meshed type Chouioia cunea Yang breeding box.
Further, the time for transferring the net-covered type Chouioia cunea Yang breeding box to a bee breeding frame to perform sterilization by the irradiation of a sterilizing lamp is more than or equal to 15 minutes.
Further, the net-covered type Chouioia cunea Yang breeding box comprises a box body and a box cover, wherein the top surface of the box cover is provided with a gauze, and the meshes of the gauze correspond to the size of the Chouioia cunea Yang; the box cover covers the box body.
Furthermore, the box body of the net-covered type Chouioia cunea Yang breeding box is made of wood materials.
Further, the inoculation workbench comprises a workbench surface, and the workbench surface is 1 m high; a sterilizing lamp is arranged above the worktable, and the distance between a sterilizing lamp tube and the worktable is 1 m.
According to the method for controlling the bad pupa disease of the Chouioia cunea Yang breeding, provided by the invention, the main link of Chouioia cunea Yang inoculation is sterilized and disinfected according to the propagation mechanism design of the bad pupa pathogenic bacteria, so that the propagation of the bad pupa pathogenic bacteria in the bee inoculation process is effectively inhibited; the use of the net-covered type Chouioia cunea Yang breeding box with the gauze at the top makes full use of the phototaxis of the Chouioia cunea Yang to ensure that the abdomen of the Chouioia cunea Yang is upward laid on the gauze, so that the ovipositor of the Chouioia cunea Yang can be sterilized and disinfected by disinfector and ultraviolet rays more conveniently, and the sterilizing and disinfecting effects are improved; the breeding box is communicated with the inside and the outside, which is beneficial to controlling the humidity in the box and creating the environment which is not beneficial to the silkworm chrysalis to rot. The method for controlling the bad pupa disease of the Chouioia cunea Yang bred by the Chouioia cunea Yang provided by the invention can improve breeding efficiency, reduce economic loss and reduce the bad pupa breeding rate by more than 30%, thereby achieving the purpose of stably providing the natural enemy insect Chouioia cunea Yang, and making more contributions to insect control and ecological environment protection by insects.
Drawings
The accompanying drawings partially disclose embodiments of the present invention, in which,
FIG. 1 is a schematic structural diagram of a meshed type Chouioia cunea Yang breeding box for white moth of the present invention;
FIG. 2 is a schematic view of the present invention for sterilizing and disinfecting a hatched Chouioia cunea Yang in a bee-warming bottle;
FIG. 3 is a schematic structural view of the inoculation according to the present invention;
FIG. 4 is a schematic view of the sterilization and disinfection of the Chouioia cunea Yang which is inserted into the mesh-covered Chouioia cunea Yang breeding box according to the present invention;
fig. 5 is a schematic structural view of the bee breeding rack of the present invention.
Detailed Description
As shown in attached figures 1-5, the method for controlling the bad pupa disease of the Chouioia cunea Yang breeding of the white moth comprises the steps of putting a seed pupa 8 into a bee-warming bottle 1 made of a transparent plastic bottle, plugging a bottle stopper made of absorbent cotton into the bee-warming bottle, controlling the environmental temperature below 28 ℃, and waiting for the Chouioia cunea Yang 3 to emerge from the seed pupa;
when the Chouioia cunea Yang emerges from the pupae and breaks the shell for the next day or the third day, the temperature is controlled between 20 ℃ and 25 ℃, the bottle stopper is opened, the disinfectant is diluted, and then the Chouioia cunea Yang which has emerged in the Chouioia cunea Yang is quickly sprayed and disinfected by a sprayer 9 with better atomization at a distance of more than 1 meter from the Chouian Yang bee bottle; the bottle stopper is plugged back, and the bottle mouth is kept standing for 30 minutes;
manufacturing a net-covered type Chouioia cunea Yang breeding box 2, wherein the net-covered type Chouioia cunea Yang breeding box is similar to a traditional Chinese furniture 'holding box', and is divided into a box body 6 and a box cover 7, wherein the top surface of the box cover is a gauze 10, and four vertical surfaces of the box cover, 4 vertical surfaces of the box body and the bottom surface of the box body are all wood; uniformly placing the silkworm chrysalis 11 into a box body of a net-covered type Chouioia cunea Yang breeding box;
a tubular sterilizing lamp 5 is hung above an inoculation workbench 4 with the height of 1 m, and the distance between the sterilizing lamp tube and the floor is about 2 m. An operator wears an ultraviolet protective mask and linear gloves, wears long-sleeve work clothes and trousers without exposing skin, inoculates the Chouioia cunea Yang under a sterilizing lamp, beats a bee warming bottle and shakes a cotton wool plug to uniformly scatter the eclosion white moth Chouioia cunea Yang and the breeding pupae into the net-covered type Chouioia cunea Yang breeding box body 6, quickly covers a box cover 7 and seals gaps by using an adhesive tape;
placing the net-covered type Chouioia cunea Yang breeding box with the inoculated apis cerana on the floor or on an operation table, controlling the temperature to be between 20 and 23 ℃, and enabling the Chouioia cunea Yang to be laid on a gauze 10 of a box cover 7 due to phototaxis and the belly to be upward; the distance between the mesh-covered type Chouioia cunea Yang breeding box and the mesh-covered type Chouioia cunea Yang breeding box is about 1 m, an atomizer with good atomization is held to face a gauze 10 of the Chouioia cunea Yang breeding box 2, diluted disinfectant is used for fast spraying, and therefore the abdomen of the Chouioia cunea Yang and an ovipositor are sterilized and disinfected;
transferring the net-covered type Chouioia cunea Yang breeding box sterilized by the disinfectant to a bee breeding rack provided with a sterilizing lamp. When the sterilizing lamp is arranged above the bee breeding rack, the distance between the lamp tube and the mesh covering type hyphantria cunea breeding box gauze 10 is about 0.1 meter. When the sterilizing lamp is arranged on the rear wall, the distance between the lamp tube and the front edge of the top surface of the mesh-covered type Chouioia cunea Yang breeding box is less than 1 meter. Controlling the temperature at about 25 ℃, sterilizing for more than 15 minutes by irradiating a sterilizing lamp, turning off the sterilizing lamp, and keeping the dark light to ensure that the apis cerana smith are concentrated in the silkworm pupae to lay eggs. After that, the sterilizing lamp is turned on for 15 minutes every day, the adhesive tape is torn off after 1 to 2 days, the box cover 7 is taken down, and the breeding box 6 is kept open and is transferred to the temperature and humidity control culture stage.
The temperature of the culture stage can be adjusted between 10-28 deg.C according to the releasing time of bee, and the humidity can be controlled between 50-80%. The bee time can be calculated according to an effective temperature accumulating method provided by the technical regulation for artificial breeding and application of Chouioia cunea Yang to ensure that the bee time is matched with the pupation time of lepidopterous larvae such as fall webworms and the like; the actual release time is 3-5 days earlier than the bee-out time calculated by an effective temperature accumulating method, so that the phenomenon that the wasps are released before being released due to the fact that the wasps cannot be released in rainy days or the distribution and reception delay is caused is prevented. Certainly, if the conditions permit, when the Chouioia cunea Yang develops to the old larva stage, the parasitized tussah pupae can be transferred into a refrigerator or a refrigerating chamber at 0-3 ℃ for storage, and released at a proper time.
Claims (9)
1. A method for controlling bad pupa disease of Chouioia cunea Yang breeding is characterized by comprising the following steps:
the method comprises the following steps: putting the breeding pupae (8) into the bee warming bottle (1), covering the bottle stopper, and waiting for the Chouioia cunea Yang (3) to emerge from the breeding pupae;
step two: opening the bottle stopper to sterilize the emerged Chouioia cunea Yang in the thermos bottle;
step three: placing the box body of the net-covered type Chouioia cunea Yang breeding box (2) in which the silkworm chrysalis (11) is placed on an inoculation workbench;
step four: opening a sterilizing lamp (5) above an inoculation workbench, beating a warm bee bottle under the irradiation of the sterilizing lamp, uniformly scattering emerged Chouioia cunea Yang into a box body (6) of a net-covered Chouia cunea Yang breeding box for inoculation, and covering a box cover (7) with a gauze (10) at the top;
step five: sterilizing and disinfecting the newly-inoculated Chouioia cunea Yang in the net-covered Chouioia cunea breeding box;
step six: transferring the net-covered type Chouioia cunea Yang breeding box to a Chouioia cunea frame for sterilizing by irradiation of a sterilizing lamp;
step seven: turning off the sterilizing lamp, and keeping the dark light to enable the breeding bees to lay eggs in the silkworm pupa body;
step eight: and turning on a sterilizing lamp for 15 minutes every day, tearing off the adhesive tape after 1-2 days, taking off the box cover, opening the net-covered type Chouioia cunea Yang breeding box, and turning to a temperature and humidity control culture stage.
2. The wasp warming bottle for the control method of the Chouioia cunea Yang propagation pupa necrosis of the Chouioia cunea according to claim 1, wherein the wasp warming bottle is a transparent plastic bottle, and the bottle stopper is a cotton ball.
3. The method for controlling the breeding of the pupal disease of the Chouioia cunea Yang as claimed in claim 1, wherein the means for sterilizing the Chouioia cunea Yang, which has emerged in the bottle, is spray sterilization; after the disinfectant is diluted, the disinfectant is quickly sprayed by an atomizer (9) at a position 1 m away from the mouth of the wasp bottle.
4. The method for controlling the propagation of the bad pupa disease of the Chouioia cunea Yang as claimed in claim 1, wherein the further inoculation step is performed after the Chouioia cunea Yang which has emerged in the warm bee bottle (1) is sterilized and disinfected for 30 minutes.
5. The method for controlling bad pupa disease of Chouioia cunea Yang breeding of Chouioia cunea Yang according to claim 1, wherein the method for sterilizing and disinfecting Chouioia cunea Yang just inoculated into the mesh-covered Chouioia cunea Yang breeding box is spray sterilization and disinfection; under the irradiation of a sterilizing lamp (5), the diluted disinfectant is quickly sprayed by an atomizer at a distance of 1 m from a gauze on the top surface of the meshed type Chouioia cunea Yang breeding box (6).
6. The method for controlling bad pupa disease of Chouioia cunea Yang breeding of Chouioia cunea Yang as claimed in claim 1, wherein the time period for sterilizing by the germicidal lamp is not less than 15 minutes when the mesh-covered Chouioia cunea Yang breeding box is transferred to a Chouioia cunea Yang frame.
7. The meshed type Chouioia cunea Yang breeding box for the Chouioia cunea Yang breeding bad pupa disease control method according to claim 1, comprising a box body and a box cover, wherein the top surface of the box cover is provided with a gauze, and the meshes of the gauze correspond to the size of the Chouioia cunea Yang; the box cover covers the box body.
8. The net-covered type Chouioia cunea Yang breeding box for the Chouioia cunea Yang breeding bad pupa disease control method according to claim 1, wherein the box is made of wood.
9. The inoculation workbench used in the control method for the breeding of the bad pupae disease of the Chouioia cunea Yang according to claim 1, which comprises a workbench top, wherein the workbench top is 1 m high; the sterilizing lamp is arranged above the worktable surface, and the distance between the sterilizing lamp tube and the worktable surface is 1 m.
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Cited By (1)
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| CN114847238A (en) * | 2022-04-15 | 2022-08-05 | 寿光市林业发展中心 | Chouioia cunea Yang breeds and uses device |
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| CN112868607B (en) | 2022-12-16 |
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