CN112840036A - 基因芯片及其制备方法 - Google Patents

基因芯片及其制备方法 Download PDF

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Publication number
CN112840036A
CN112840036A CN201880095816.3A CN201880095816A CN112840036A CN 112840036 A CN112840036 A CN 112840036A CN 201880095816 A CN201880095816 A CN 201880095816A CN 112840036 A CN112840036 A CN 112840036A
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China
Prior art keywords
polymer
chip
reaction
polymer film
chip carrier
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Pending
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CN201880095816.3A
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English (en)
Inventor
王照辉
王卉
邢承美
李汉东
章文蔚
沙夫托·杰
龚梅花
杨晋
罗银玲
张振华
李元
江雪芹
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BGI Shenzhen Co Ltd
Shenzhen BGI Life Science Research Institute
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BGI Shenzhen Co Ltd
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Publication of CN112840036A publication Critical patent/CN112840036A/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00628—Ionic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00646—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/00648—Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of solid beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

提供了一种基因芯片及其制备方法。该基因芯片包括芯片载体以及组装于芯片载体上的DNA纳米球。该基因芯片进一步包括聚合物膜，该聚合物膜形成于芯片载体上并包裹DNA纳米球，其中该聚合物膜包括正电聚合物膜、改性正电聚合物膜、两性离子聚合物膜以及复合聚合物膜中的至少一种，复合聚合物膜通过正电聚合物和负电聚合物层层自组装形成。该基因芯片可降低表面干燥或表面非特异性吸附。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201880095816.3A 2018-08-15 2018-08-15 基因芯片及其制备方法 Pending CN112840036A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/100692 WO2020034122A1 (zh) 2018-08-15 2018-08-15 基因芯片及其制备方法

Publications (1)

Publication Number Publication Date
CN112840036A true CN112840036A (zh) 2021-05-25

Family

ID=69524625

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CN201880095816.3A Pending CN112840036A (zh) 2018-08-15 2018-08-15 基因芯片及其制备方法

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US (1) US20210162365A1 (zh)
EP (1) EP3839060B1 (zh)
CN (1) CN112840036A (zh)
WO (1) WO2020034122A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113290365A (zh) * 2021-07-08 2021-08-24 苏州鼎纳自动化技术有限公司 一种杂交膜组装设备
WO2023202368A1 (zh) * 2022-04-20 2023-10-26 南方科技大学 核酸的封装及去封装方法和核酸存储微流控芯片

Citations (8)

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JP2000295990A (ja) * 1999-02-08 2000-10-24 Fuji Photo Film Co Ltd Ｄｎａ断片の固定方法、ｄｎａチップおよびｄｎａ断片の検出方法
JP2001272402A (ja) * 2000-03-24 2001-10-05 Fuji Photo Film Co Ltd Ｄｎａチップの製造方法およびｄｎａチップ
US20020090640A1 (en) * 1999-02-08 2002-07-11 Satoru Kuhara DNA chip and its preparation
CN1362991A (zh) * 1999-07-23 2002-08-07 宝酒造株式会社 Dna芯片
WO2009109753A2 (en) * 2008-03-04 2009-09-11 Kalim Mir Multiplex selection and sequencing
US20130203629A1 (en) * 2010-10-18 2013-08-08 Centre National De La Recherche Scientifique Method for functionalizing surfaces for analyte detection
CN103898194A (zh) * 2012-12-24 2014-07-02 深圳国际旅行卫生保健中心 光交联功能化的基因芯片及其制备方法及检测试剂盒
CN108060460A (zh) * 2018-01-17 2018-05-22 深圳华大生命科学研究院 一种small RNA的文库构建及测序方法

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US9671344B2 (en) * 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
US10837879B2 (en) * 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
TWI565532B (zh) * 2012-08-07 2017-01-11 國立交通大學 奈米球溶液塗佈方法與其應用
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Patent Citations (8)

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JP2000295990A (ja) * 1999-02-08 2000-10-24 Fuji Photo Film Co Ltd Ｄｎａ断片の固定方法、ｄｎａチップおよびｄｎａ断片の検出方法
US20020090640A1 (en) * 1999-02-08 2002-07-11 Satoru Kuhara DNA chip and its preparation
CN1362991A (zh) * 1999-07-23 2002-08-07 宝酒造株式会社 Dna芯片
JP2001272402A (ja) * 2000-03-24 2001-10-05 Fuji Photo Film Co Ltd Ｄｎａチップの製造方法およびｄｎａチップ
WO2009109753A2 (en) * 2008-03-04 2009-09-11 Kalim Mir Multiplex selection and sequencing
US20130203629A1 (en) * 2010-10-18 2013-08-08 Centre National De La Recherche Scientifique Method for functionalizing surfaces for analyte detection
CN103898194A (zh) * 2012-12-24 2014-07-02 深圳国际旅行卫生保健中心 光交联功能化的基因芯片及其制备方法及检测试剂盒
CN108060460A (zh) * 2018-01-17 2018-05-22 深圳华大生命科学研究院 一种small RNA的文库构建及测序方法

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113290365A (zh) * 2021-07-08 2021-08-24 苏州鼎纳自动化技术有限公司 一种杂交膜组装设备
CN113290365B (zh) * 2021-07-08 2021-09-28 苏州鼎纳自动化技术有限公司 一种杂交膜组装设备
WO2023202368A1 (zh) * 2022-04-20 2023-10-26 南方科技大学 核酸的封装及去封装方法和核酸存储微流控芯片

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WO2020034122A1 (zh) 2020-02-20
EP3839060A4 (en) 2022-03-23
EP3839060B1 (en) 2022-10-12
EP3839060A1 (en) 2021-06-23
US20210162365A1 (en) 2021-06-03

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