CN112840022A - 核酸的体外检测 - Google Patents
核酸的体外检测 Download PDFInfo
- Publication number
- CN112840022A CN112840022A CN201980067842.XA CN201980067842A CN112840022A CN 112840022 A CN112840022 A CN 112840022A CN 201980067842 A CN201980067842 A CN 201980067842A CN 112840022 A CN112840022 A CN 112840022A
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- nucleic acid
- sensor system
- sensor
- hybridization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862718427P | 2018-08-14 | 2018-08-14 | |
| US62/718,427 | 2018-08-14 | ||
| PCT/US2019/046497 WO2020037038A1 (en) | 2018-08-14 | 2019-08-14 | In vitro detection of nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112840022A true CN112840022A (zh) | 2021-05-25 |
Family
ID=69525831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980067842.XA Pending CN112840022A (zh) | 2018-08-14 | 2019-08-14 | 核酸的体外检测 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US12644148B2 (enExample) |
| EP (1) | EP3837362A4 (enExample) |
| JP (1) | JP2021533771A (enExample) |
| KR (1) | KR20210043605A (enExample) |
| CN (1) | CN112840022A (enExample) |
| AU (1) | AU2019321447A1 (enExample) |
| BR (1) | BR112021002609A2 (enExample) |
| CA (1) | CA3109392A1 (enExample) |
| IL (1) | IL280603A (enExample) |
| MX (1) | MX2021001685A (enExample) |
| SG (1) | SG11202101405RA (enExample) |
| WO (1) | WO2020037038A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117330755A (zh) * | 2023-08-25 | 2024-01-02 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4200438A1 (en) * | 2020-08-18 | 2023-06-28 | Sherlock Biosciences, Inc. | Hybridisation-based sensor systems and probes |
| WO2022046979A1 (en) * | 2020-08-26 | 2022-03-03 | Arizona Board Of Regents On Behalf Of Arizona State University | Rapid and low-cost nucleic acid detection using translation-based sequence verification assays |
| WO2023283622A1 (en) | 2021-07-08 | 2023-01-12 | Montana State University | Crispr-based programmable rna editing |
| US11814689B2 (en) | 2021-07-21 | 2023-11-14 | Montana State University | Nucleic acid detection using type III CRISPR complex |
| KR102818567B1 (ko) * | 2022-05-31 | 2025-06-11 | 충남대학교 산학협력단 | 타겟 핵산 검출용 조성물 및 이의 용도 |
| EP4547865A1 (en) | 2022-07-01 | 2025-05-07 | Sherlock Biosciences, Inc. | Ambient temperature nucleic acid amplification and detection |
| KR20250074706A (ko) * | 2023-11-15 | 2025-05-28 | 대한민국(방위사업청장) | 무세포 단백질 합성과 재귀반사신호에 기반한 표적물질 검출방법 |
| KR20250085854A (ko) * | 2023-12-04 | 2025-06-13 | 충남대학교산학협력단 | 타겟 핵산 검출용 조성물 및 이의 용도 |
| WO2026015653A2 (en) | 2024-07-09 | 2026-01-15 | Sherlock Biosciences, Inc. | Power-free fluidic device |
| WO2026104594A1 (en) * | 2024-11-15 | 2026-05-21 | Eth Zurich | Biosensor |
Citations (6)
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| US6197508B1 (en) * | 1990-09-12 | 2001-03-06 | Affymetrix, Inc. | Electrochemical denaturation and annealing of nucleic acid |
| US20040171047A1 (en) * | 2002-05-22 | 2004-09-02 | Dahl Gary A. | Target-dependent transcription |
| US20110039257A1 (en) * | 2006-04-03 | 2011-02-17 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| CN102186997A (zh) * | 2008-08-15 | 2011-09-14 | 卡斯凯德生物体系股份有限公司 | 检测核酸 |
| US20140073517A1 (en) * | 2010-02-23 | 2014-03-13 | Rheonix, Inc. | Self-contained biological assay apparatus, methods, and applications |
| WO2014074648A2 (en) * | 2012-11-06 | 2014-05-15 | President And Fellows Of Harvard College | Riboregulator compositions and methods of use |
Family Cites Families (38)
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| US8148065B1 (en) | 1988-04-06 | 2012-04-03 | City Of Hope | Ligation amplification of nucleic acid sequences |
| US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
| AU6027794A (en) | 1993-01-15 | 1994-08-15 | Public Health Research Institute Of The City Of New York, Inc., The | Diagnostic assays and kits for rna using rna binary probes and an rna-directed rna ligase |
| EP0682716A4 (en) | 1993-01-15 | 1999-10-27 | New York Health Res Inst | RNA ASSAYS USING BINARY RNA PROBES AND A RIBOZYME-LIGASE. |
| US6593086B2 (en) | 1996-05-20 | 2003-07-15 | Mount Sinai School Of Medicine Of New York University | Nucleic acid amplification methods |
| DE69528670T2 (de) | 1994-12-23 | 2004-03-11 | Dade Behring Inc., Deerfield | Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung |
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| HUE058149T2 (hu) | 2014-01-31 | 2022-07-28 | Swift Biosciences Inc | Javított eljárások DNS-szubsztrátok feldolgozására |
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| PT3464628T (pt) | 2016-06-06 | 2024-06-03 | Redvault Biosciences Lp | Construções repórter de alvo e suas utilizações |
-
2019
- 2019-08-14 SG SG11202101405RA patent/SG11202101405RA/en unknown
- 2019-08-14 BR BR112021002609-6A patent/BR112021002609A2/pt unknown
- 2019-08-14 KR KR1020217006686A patent/KR20210043605A/ko not_active Ceased
- 2019-08-14 AU AU2019321447A patent/AU2019321447A1/en not_active Abandoned
- 2019-08-14 MX MX2021001685A patent/MX2021001685A/es unknown
- 2019-08-14 CN CN201980067842.XA patent/CN112840022A/zh active Pending
- 2019-08-14 JP JP2021507661A patent/JP2021533771A/ja active Pending
- 2019-08-14 WO PCT/US2019/046497 patent/WO2020037038A1/en not_active Ceased
- 2019-08-14 CA CA3109392A patent/CA3109392A1/en active Pending
- 2019-08-14 EP EP19850135.5A patent/EP3837362A4/en active Pending
- 2019-08-14 US US17/268,010 patent/US12644148B2/en active Active
-
2021
- 2021-02-02 IL IL280603A patent/IL280603A/en unknown
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6197508B1 (en) * | 1990-09-12 | 2001-03-06 | Affymetrix, Inc. | Electrochemical denaturation and annealing of nucleic acid |
| US20040171047A1 (en) * | 2002-05-22 | 2004-09-02 | Dahl Gary A. | Target-dependent transcription |
| US20110039257A1 (en) * | 2006-04-03 | 2011-02-17 | Promega Corporation | Permuted and nonpermuted luciferase biosensors |
| CN102186997A (zh) * | 2008-08-15 | 2011-09-14 | 卡斯凯德生物体系股份有限公司 | 检测核酸 |
| US20140073517A1 (en) * | 2010-02-23 | 2014-03-13 | Rheonix, Inc. | Self-contained biological assay apparatus, methods, and applications |
| WO2014074648A2 (en) * | 2012-11-06 | 2014-05-15 | President And Fellows Of Harvard College | Riboregulator compositions and methods of use |
Non-Patent Citations (2)
| Title |
|---|
| S D WHARAM 等: "Specific detection of DNA and RNA targets using a novel isothermal nucleic acid amplification assay based on the formation of a three-way junction structure", NUCLEIC ACIDS RES ., vol. 29, no. 11, pages 1 - 8, XP002258951, DOI: 10.1093/nar/29.11.e54 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117330755A (zh) * | 2023-08-25 | 2024-01-02 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
| CN117330755B (zh) * | 2023-08-25 | 2024-03-26 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2019321447A1 (en) | 2021-02-25 |
| CA3109392A1 (en) | 2020-02-20 |
| IL280603A (en) | 2021-03-25 |
| JP2021533771A (ja) | 2021-12-09 |
| KR20210043605A (ko) | 2021-04-21 |
| MX2021001685A (es) | 2021-05-27 |
| EP3837362A1 (en) | 2021-06-23 |
| WO2020037038A1 (en) | 2020-02-20 |
| US20210164025A1 (en) | 2021-06-03 |
| US12644148B2 (en) | 2026-06-02 |
| BR112021002609A2 (pt) | 2021-05-11 |
| SG11202101405RA (en) | 2021-03-30 |
| EP3837362A4 (en) | 2022-05-11 |
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