CN112795523A - Preparation method of cowshed ammonia inhibitor - Google Patents
Preparation method of cowshed ammonia inhibitor Download PDFInfo
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- CN112795523A CN112795523A CN202110273391.4A CN202110273391A CN112795523A CN 112795523 A CN112795523 A CN 112795523A CN 202110273391 A CN202110273391 A CN 202110273391A CN 112795523 A CN112795523 A CN 112795523A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/77—Liquid phase processes
- B01D53/78—Liquid phase processes with gas-liquid contact
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01D2258/00—Sources of waste gases
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Abstract
The invention discloses a preparation method of an ammonia inhibitor for cowsheds, and belongs to the technical field of cowshed ammonia treatment. The method comprises the following steps: adding Terminalia catappa leaves, orange peel and shaddock peel into water, soaking for 3-5 days, and filtering to obtain Terminalia catappa leaf extract; adding 2g of monopotassium phosphate into every 1L of the terminalia seed leaf leachate, adding monopotassium phosphate into the terminalia seed leaf leachate obtained in the step one, and mixing to obtain a mixed solution; preparing a nitrobacteria culture solution; according to the following steps: 1000, and fully mixing the obtained nitrobacteria culture solution and the obtained potassium dihydrogen phosphate mixed solution to obtain the ammonia inhibitor. The method has the advantages of easily available raw materials, low cost, environmental friendliness, no pollution, simple operation method, obvious and lasting ammonia removal effect and suitability for wide popularization.
Description
Technical Field
The invention relates to the technical field of cowshed ammonia gas treatment, in particular to a preparation method of an ammonia gas inhibitor for cowsheds.
Background
The ammonia gas in the cowshed is a toxic gas which causes serious harm to the physical and psychological health of livestock and feeding personnel, and the ammonia (NH3) discharged by the animal feeding is a main air and water pollutant causing water eutrophication, soil acidity and aerosol formation. Ammonia can react with water in the respiratory tract of livestock and poultry to generate water-soluble corrosive NH4+ ions, further the respiratory tract mucosa system is damaged, the immunity of the livestock and poultry is reduced, so that the livestock and poultry are easily infected by germs, serious disasters are brought to livestock and poultry breeding, ammonia enters a blood circulation system through a respiratory system and is combined with hemoglobin, heme is converted into hematin, the oxygen carrying capacity of the hemoglobin, the blood alkali storage capacity and the oxidation performance of the heme are reduced, further the anemia and the tissue hypoxia of the livestock and poultry are caused, and the resistance of organisms to diseases is reduced. Studies have shown that high concentrations of ammonia in livestock houses can also have an effect on the growth of livestock animals, especially in their young growth period.
How to reduce the ammonia concentration of the cowshed is always an important method for improving the cowshed environment, increasing the cowshed benefit and optimizing the working environment of workers, but the method for reducing the ammonia volatilization of cow dung in the cowshed is high in cost and difficult to popularize practically, taking the use of urease inhibitor NBPT as an example, the market price of one kilogram of NBPT is about 500 yuan, the method is completely not in line with the practical production requirement, and when chemicals such as ferrous sulfate reduce the ammonia volatilization, a potential pollution source can be caused, and when the cow dung is applied to the field, the environmental problems such as land acidification can be caused. The method for feeding probiotics, urease inhibitor and the like has slow effect, and the feeding of the urease inhibitor is proved to have potential safety hazards of livestock health. Therefore, how to remove ammonia by a low-cost and environment-friendly way is a technical problem which troubles practitioners.
Disclosure of Invention
The invention aims to provide a method for preparing an ammonia inhibitor for cowsheds, which aims to solve the problems in the background art.
The preparation method of the cowshed ammonia inhibitor comprises the following steps:
the method comprises the following steps: adding the mature and dried olive kernel leaves, orange peel and shaddock peel into water, soaking for 3-5 days, and filtering to obtain olive kernel leaf leachate;
step two: adding 2g of monopotassium phosphate into every 1L of the terminalia seed leaf leachate, adding monopotassium phosphate into the terminalia seed leaf leachate obtained in the step one, and mixing to obtain a mixed solution;
step three: taking distilled water, adding a mixed solution of 10 g of peptone, 10 g of sodium chloride and 5 g of yeast extract into 1L of distilled water, adding sodium hydroxide into the mixed solution to adjust the pH value to be equal to 7, sterilizing, adding a nitrifying bacteria mother solution, and performing shake culture at room temperature for 3-4 days to obtain a nitrifying bacteria culture solution;
step four: according to the following steps: and (1000) fully mixing the nitrifying bacteria culture solution obtained in the third step and the potassium dihydrogen phosphate mixed solution obtained in the second step to obtain the ammonia inhibitor.
In the first step, 80-120 g of mature and dried olive kernel leaves, 40-60 g of dried orange peels and 40-70 g of dried shaddock peels are taken, added into 10-15L of water and soaked for 3-5 days, the olive kernel leaves are stirred once a day during the soaking period, and filtered to obtain olive kernel leaf leachate.
Taking 100g of mature and dried olive kernel leaves, 50g of orange peel and 50g of shaddock peel, adding into 10L of water, and soaking for 5 days.
In the first step, 120g of ripe and dried olive kernel leaves, 60g of dried orange peel and 70g of dried shaddock peel are taken and added into 15L of water to be soaked for 3 days.
In the first step, 80g of ripe and dried olive kernel leaves, 40g of orange peel and 40g of shaddock peel are taken and added into 10L of water for soaking for 4 days.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the raw materials are easy to obtain and convenient to store, the olive kernel leaves are leaves of common tree species in the south, the monarch tree is a common street greening tree species and widely grows in the city of the southern province, fallen leaves are easy to obtain and low in cost, nitrobacteria are easy to cultivate, the cultivating cost is low, the cultivating period is extremely short, and potassium dihydrogen phosphate is the most widely used agricultural fertilizer in agriculture and low in price compared with the traditional ammonia removal agents such as urease inhibitors, active carbon, ferrous sulfate and the like, and the cost is low.
2. The ammonia inhibitor prepared by the invention is green, safe and harmless: terminalia leaves are used as common fallen leaves of trees and are very easy to degrade in the natural environment, nitrobacteria are used as microorganisms commonly existing in nature and widely exist in soil and water body environment, and do not have toxic action on human and livestock, monopotassium phosphate is one of the most commonly used fertilizers, and human uses the fertilizer for more than one hundred years, so that the safety of the fertilizer is self-evident, and the pesticide can also increase the fertilizer efficiency of cow dung.
3. Experiments prove that the ammonia inhibitor prepared by the invention has obvious inhibition effect.
In conclusion, the invention has the advantages of simple and easily obtained raw materials, low cost, environmental protection, no public nuisance, simple operation method, obvious and durable ammonia removal effect and suitability for wide popularization.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
Example 1
The preparation method of the cowshed ammonia inhibitor comprises the following steps:
the method comprises the following steps: taking 100g of mature and dried olive kernel leaves, 50g of dried orange peel and 50g of dried shaddock peel, adding into 10L of water, soaking for 5 days, stirring once a day during the soaking period, and filtering to obtain olive kernel leaf leachate;
step two: adding 2g of monopotassium phosphate into every 1L of the terminalia seed leaf leachate, adding monopotassium phosphate into the terminalia seed leaf leachate obtained in the step one, and mixing to obtain a mixed solution;
step three: taking distilled water, adding a mixed solution of 10 g of peptone, 10 g of sodium chloride and 5 g of yeast extract into 1L of distilled water, adding sodium hydroxide into the mixed solution to adjust the pH value to be equal to 7, sterilizing, adding a nitrifying bacteria mother solution, and performing shake culture at room temperature for 3-4 days to obtain a nitrifying bacteria culture solution;
step four: according to the following steps: and (1000) fully mixing the nitrifying bacteria culture solution obtained in the third step and the potassium dihydrogen phosphate mixed solution obtained in the second step to obtain the ammonia inhibitor.
The prepared ammonia inhibitor is sprayed on the accumulation part of the cow dung and the cow urine by using a spray can, the effective time is usually more than 50 hours, and the effect is gradually reduced later.
Example 2
The present embodiment is different from embodiment 1 in that: taking 120g of mature and dried olive kernel leaves, 60g of dried orange peel and 70g of dried shaddock peel, adding into 15L of water, soaking for 3 days, and filtering to obtain olive kernel leaf leachate.
Example 3
The present embodiment is different from embodiment 1 in that: taking 80g of mature and dried olive kernel leaves, 40g of orange peel and 40g of shaddock peel, adding into 10L of water, soaking for 4 days, and filtering to obtain olive kernel leaf leachate.
Experimental example:
to demonstrate the effectiveness of the preferred embodiment 1, i performed the following experiments: the fresh cow dung-urine mixture was divided into three groups of 4 replicates, each: a non-treatment group, a NBPT urease inhibitor treatment group and an inhibitor treatment group prepared in the embodiment, wherein 300 g of a feces-urine mixture is repeatedly weighed in each group, and 200 ml of water is added in the non-treatment group; the NBPT urease inhibitor treatment group was added with 200 ml of water and two grams of urease inhibitor; the inhibitor treatment group prepared in this example was dosed with 200 ml of the drug. The data were continuously tracked and measured for 7 days by using an ammonia gas volatilization detector, and the experimental results were as follows:
as shown in the above table: the inhibitor prepared in the embodiment has a remarkable effect, compared with the non-treated group, the ammonia volatilization amount is almost inhibited by 90% in the first day, the inhibition effect is slowly reduced in the later six days, but the ammonia volatilization amount is also inhibited by 30% in the seventh day, and compared with the urease inhibitor, the effect in the first six days is even remarkably superior to that of the urease inhibitor except that the effect in the seventh day is basically the same.
The above description is only exemplary of the invention, and any modification, equivalent replacement, and improvement made within the spirit and principle of the invention should be included in the protection scope of the invention.
Claims (5)
1. The preparation method of the cowshed ammonia inhibitor is characterized by comprising the following steps:
the method comprises the following steps: adding the mature and dried olive kernel leaves, orange peel and shaddock peel into water, soaking for 3-5 days, and filtering to obtain olive kernel leaf leachate;
step two: adding 2g of monopotassium phosphate into every 1L of the terminalia seed leaf leachate, adding monopotassium phosphate into the terminalia seed leaf leachate obtained in the step one, and mixing to obtain a mixed solution;
step three: taking distilled water, adding a mixed solution of 10 g of peptone, 10 g of sodium chloride and 5 g of yeast extract into 1L of distilled water, adding sodium hydroxide into the mixed solution to adjust the pH value to be equal to 7, sterilizing, adding a nitrifying bacteria mother solution, and performing shake culture at room temperature for 3-4 days to obtain a nitrifying bacteria culture solution;
step four: according to the following steps: and (1000) fully mixing the nitrifying bacteria culture solution obtained in the third step and the potassium dihydrogen phosphate mixed solution obtained in the second step to obtain the ammonia inhibitor.
2. The preparation method of the cowshed ammonia inhibitor as defined in claim 1, which is characterized in that: in the first step, 80-120 g of mature and dried olive kernel leaves, 40-60 g of dried orange peels and 40-70 g of dried shaddock peels are taken, added into 10-15L of water and soaked for 3-5 days, the olive kernel leaves are stirred once a day during the soaking period, and filtered to obtain olive kernel leaf leachate.
3. The preparation method of the cowshed ammonia inhibitor as claimed in claim 2, characterized in that: taking 100g of mature and dried olive kernel leaves, 50g of orange peel and 50g of shaddock peel, adding into 10L of water, and soaking for 5 days.
4. The preparation method of the cowshed ammonia inhibitor as claimed in claim 2, characterized in that: in the first step, 120g of ripe and dried olive kernel leaves, 60g of dried orange peel and 70g of dried shaddock peel are taken and added into 15L of water to be soaked for 3 days.
5. The preparation method of the cowshed ammonia inhibitor as claimed in claim 2, characterized in that: in the first step, 80g of ripe and dried olive kernel leaves, 40g of orange peel and 40g of shaddock peel are taken and added into 10L of water for soaking for 4 days.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110273391.4A CN112795523A (en) | 2021-03-12 | 2021-03-12 | Preparation method of cowshed ammonia inhibitor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110273391.4A CN112795523A (en) | 2021-03-12 | 2021-03-12 | Preparation method of cowshed ammonia inhibitor |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104431455A (en) * | 2014-11-20 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Ammonia removal bacterium liquid of chicken house |
| CN105536509A (en) * | 2015-12-22 | 2016-05-04 | 湖南普泰尔微科环保科技有限公司 | Plant extract and microbe composite deodorant |
| JP2017154064A (en) * | 2016-03-01 | 2017-09-07 | 水ｉｎｇ株式会社 | Method for producing biological active carbon stuck with nitrifying bacteria |
| CN107381829A (en) * | 2017-06-22 | 2017-11-24 | 安徽省黄淮兽药有限公司 | A kind of composite water quality improving agent used for aquiculture and its application method |
| CN110300592A (en) * | 2016-12-20 | 2019-10-01 | 瑞星菲尼克斯工业公司 | The product of Fei Shi olive kernel leaf extract and the extract containing Fei Shi olive kernel leaf |
-
2021
- 2021-03-12 CN CN202110273391.4A patent/CN112795523A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104431455A (en) * | 2014-11-20 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Ammonia removal bacterium liquid of chicken house |
| CN105536509A (en) * | 2015-12-22 | 2016-05-04 | 湖南普泰尔微科环保科技有限公司 | Plant extract and microbe composite deodorant |
| JP2017154064A (en) * | 2016-03-01 | 2017-09-07 | 水ｉｎｇ株式会社 | Method for producing biological active carbon stuck with nitrifying bacteria |
| CN110300592A (en) * | 2016-12-20 | 2019-10-01 | 瑞星菲尼克斯工业公司 | The product of Fei Shi olive kernel leaf extract and the extract containing Fei Shi olive kernel leaf |
| CN107381829A (en) * | 2017-06-22 | 2017-11-24 | 安徽省黄淮兽药有限公司 | A kind of composite water quality improving agent used for aquiculture and its application method |
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