CN112773756A - Novel anti-aging essence of stem cell factor and preparation technology thereof - Google Patents
Novel anti-aging essence of stem cell factor and preparation technology thereof Download PDFInfo
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Abstract
The invention discloses a novel anti-aging essence of stem cell factors and a preparation technology thereof, wherein the novel anti-aging essence comprises the following raw materials: the preparation method comprises the following steps of: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: and adding the diluted sheep umbilical cord blood into the placenta active extract. The anti-aging composition combining the stem cell factor and the plant extract, which is prepared by the invention, can promote the absorption of nutrient components in the skin, thereby promoting the growth of collagen, enhancing the elasticity of the skin, adding the plant components, being natural, safe and environment-friendly, and endowing the essence with remarkable anti-aging effect.
Description
Technical Field
The invention relates to the technical field of anti-aging skin care, in particular to novel anti-aging essence of stem cell factors and a preparation technology thereof.
Background
There are many methods for skin anti-aging cosmetics, such as wrinkle-removing treatment of skin by using hormone, plant extract, etc., however, these methods cannot fundamentally achieve anti-aging effect, hormone use is harmful to human body, and only short-term effect can be obtained, skin aging is aggravated by long-term use, while the effect of plant extract is not obvious, and long-term use is required.
With the continuous improvement of living standard, people pay more and more attention to health, especially skin care, skin tissue is the largest tissue organ of human body, covers on the surface of human body, and blocks the damage effect of all harmful substances in the nature on living organisms, however, with the increase of age, the functions of skin tissue organ gradually age, from the aesthetic point of view, the enthusiasm of people on life is seriously influenced; from the physiological point of view, the main reason for influencing the effective protection of internal organs of human body and skin aging is that the quantity and quality of various nutrients required in the metabolic process can not be satisfied physiologically from diet, so that the quantity of synthetic raw materials of life genetic material carrier DNA in the vital cell nucleus is gradually reduced, finally the types and quantity of the proteins regulated and synthesized by the cell nucleus are reduced, the metabolic capability of the cell is reduced along with the reduction of the life, and the phenomenon of skin over-aging is represented as follows: at present, most of products for skin care in the market only have a moisturizing effect, have limited efficacy and cannot meet the requirements of people.
Disclosure of Invention
The invention aims to provide novel anti-aging essence of stem cell factors and a preparation technology thereof, has the advantage of convenient use of the anti-aging essence, and solves the problem of inconvenient use of skin care products.
In order to achieve the purpose, the invention provides the following technical scheme: a novel anti-aging essence combining stem cell factors and plant proteins is prepared from the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps:
step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1;
step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension;
step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution;
step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence;
step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials;
step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying;
step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Preferably, in the step e, the edible cactus is sliced and dried in the sun, impurities are removed, the sliced and dried cactus is soaked in clear water for 20-30min, then the sliced and dried cactus is placed at normal temperature and smashed to prepare the cactus extract in the raw materials.
Preferably, in the step e, dried roots and rhizomes of rhodiola rosea with the height of 15-18cm, thick roots, conical shape, most fibrous roots at root necks, short rootstocks, thick shape and cylindrical shape are taken, and flower stems are withered and dug in autumn, the rough skin is removed, cleaned, dried in the sun and extracted by a water extraction method to obtain the rhodiola rosea extract in the raw materials.
Preferably, in the step e, the fresh wolfberry fruits are soaked in low-concentration soda lye at the temperature of 60 ℃ for 1-2 min, fished and placed in a dish, washed with water, dried, sun-dried or deep-baked to be dry, and then mashed to obtain the wolfberry fruits in the raw materials.
Preferably, in the step e, dried mature pericarp of rutaceae plants and cultivars thereof is picked, the pericarp is peeled, impurities are removed, water is sprayed, the pericarp is moistened, cut into threads, dried, 50 times of water by weight is added, the mixture is leached for 45min in a water bath kettle at 45 ℃, then the mixture is sterilized at 9 ℃, and the heat preservation and leaching are continued for 60min, so that the dried orange peel in the raw material can be obtained.
Preferably, in the step e, the honey is placed in a pot, the honey is heated to be boiled, then the honey is kept to be slightly boiled by slow fire, foams and waxes floating on the surface are fished out, then impurities are filtered out by gauze or gauze, the honey is diluted by boiled water, and then the honey is added into astragalus slices with the diameter of 2.5-3.5cm, the thickness of 0.2-0.4cm and light brown or tan outer surface, the mixture is uniformly stirred and moistened for 4-6h, then the astragalus slices are placed in a frying pot and fried by slow fire until the fried astragalus is uniformly deep yellow and glossy, then the astragalus slices are taken out and cooled, and the cooled astragalus slices are extracted by a supercritical extraction method, so that the fried astragalus in the raw materials are obtained.
Compared with the prior art, the invention has the following beneficial effects: the anti-aging composition combining the stem cell factor and the plant extract, which is prepared by the invention, can promote the absorption of nutrient components in the skin, thereby promoting the growth of collagen, enhancing the elasticity of the skin, adding the plant components, being natural, safe and environment-friendly, and endowing the essence with remarkable anti-aging effect.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps:
step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1;
step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension;
step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution;
step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence;
step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials;
step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying;
step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 1
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 2
In example 1, the following additional steps were added:
and e, taking edible cactus slices, drying in the sun, removing impurities, soaking in clear water for 20-30min, then placing at normal temperature for drying in the sun, and then mashing to obtain the cactus extract in the raw materials.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 3
In example 2, the following steps were added:
taking dry roots and rootstocks of the rhodiola rosea which are 15-18cm high, thick and conical, have most fibrous roots at root necks, are short in rootstocks, are thick and are cylindrical, withering the stems of the rhodiola rosea in autumn, digging, removing the thick skins, cleaning, drying in the sun, and extracting by using a water extraction method to obtain the rhodiola rosea extract in the raw materials.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 4
In example 3, the following steps were added:
and e, soaking the fresh wolfberry fruits in low-concentration soda alkali liquor at the temperature of 60 ℃ for 1-2 min, taking out, placing in a dish, washing with water, airing, drying in the sun or baking till the fruits are dry, and mashing to obtain the wolfberry fruits in the raw materials.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 5
In example 4, the following steps were added:
and e, picking dry mature pericarp of the rutaceae plant and the cultivar thereof, peeling the pericarp, removing impurities, spraying water, moistening, shredding, drying, adding 50 times of water by weight, leaching for 45min in a water bath kettle at 45 ℃, sterilizing at 9 ℃, and continuously preserving heat and leaching for 60min to obtain the dried orange peel in the raw material.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Example 6
In example 5, the following steps were added:
and e, placing the honey in a pot, heating to boil, keeping slight boiling by using slow fire, fishing out foams and waxiness floating on the surface, filtering out impurities by using gauze or gauze, diluting by using boiled water, adding the diluted honey into astragalus slices with the diameter of 2.5-3.5cm, the thickness of 0.2-0.4cm and light brown or tan outer skin, uniformly stirring, moistening for 4-6h, placing the astragalus slices in a frying pot, frying by using slow fire until the roasted astragalus is uniformly deep yellow and glossy, taking out, cooling, and extracting the cooled astragalus slices by using a supercritical extraction method to obtain the roasted astragalus in the raw material.
A novel anti-aging essence combining stem cell factors and plant proteins comprises the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
A preparation technology of a novel anti-aging essence combining stem cell factors and plant proteins comprises the following steps: step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1; step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension; step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution; step d: ultrasonically crushing a sample in an ice bath, centrifuging a stem cell extracting solution by a high-speed centrifuge for 15min at the temperature of 4 ℃ at the rotating speed of 12000r, taking supernate, subpackaging by adopting test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence; step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials; step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying; step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A novel anti-aging essence combining stem cell factors and plant proteins is characterized in that: the novel anti-aging essence consists of the following raw materials: the traditional Chinese medicine composition comprises a dry cell extracting solution, a cactus extract, a pyracantha fortuneana fruit extract, a rhodiola rosea extract, a wolfberry fruit, a mulberry, a black sesame seed, a gordon euryale seed, a sharpleaf galangal fruit, dried orange peel, a honey-fried astragalus root, a ginseng extract and epimedium herb.
2. A preparation technology of novel anti-aging essence combining stem cell factors and plant proteins is characterized in that: the method comprises the following steps:
step a: collecting the umbilical cord blood of sheep, adding an anticoagulant, keeping the temperature at 4 ℃, and diluting with normal saline, wherein the volume ratio of the umbilical cord blood of the sheep to the normal saline is 1-1.2: 1;
step b: adding the diluted sheep umbilical cord blood into the placenta active extract, wherein the volume ratio of the diluted sheep umbilical cord blood to the placenta active extract is 0.8-1.2: 1, then adding a gelatin solution with the mass ratio of 3% -6%, and the volume ratio of the gelatin solution to the placenta active extract is 0.3-0.5: 1, and performing gradient centrifugal separation to obtain a liquid phase interface cell suspension;
step c: washing the cell suspension twice by using a phosphate buffer solution to obtain a stem cell extracting solution;
step d: ultrasonically crushing the stem cell extracting solution in an ice bath, centrifuging the stem cell extracting solution by a high-speed centrifuge at the rotating speed of 12000r at the temperature of 4 ℃ for 15min, taking supernatant, subpackaging by using test tubes, and mixing the subpackaged cell crushing solutions to obtain stem cell essence;
step e: processing radix et caulis Opuntiae Dillenii, fructus Pyracanthae, radix Rhodiolae, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix, and herba Epimedii to obtain plant materials;
step f: mixing radix et caulis Opuntiae Dillenii extract, fructus Pyracanthae extract, radix Rhodiolae extract, fructus Lycii, Mori fructus, semen Sesami Niger, semen euryales, fructus Alpinae Oxyphyllae, pericarpium Citri Tangerinae, radix astragali Preparata, Ginseng radix extract, herba Epimedii and deionized water, heating to 75-80 deg.C, homogenizing, and emulsifying;
step g: and cooling to room temperature, adding the stem cell essence, uniformly stirring, and performing vacuum degassing to obtain the novel anti-aging essence stock solution.
3. The preparation technology of the novel anti-aging essence combining stem cell factor and plant protein as claimed in claim 2, is characterized in that: and e, taking edible cactus slices, drying in the sun, removing impurities, soaking in clear water for 20-30min, then placing at normal temperature for drying in the sun, and then mashing to obtain the cactus extract in the raw materials.
4. The preparation technology of the novel anti-aging essence combining stem cell factor and plant protein as claimed in claim 2, is characterized in that: and e, taking dried roots and rhizomes of the rhodiola rosea which are 15-18cm high, thick and conical in root, short in rhizome, thick and cylindrical in root neck, withering the stems in autumn, digging, removing the thick skin, cleaning, drying in the sun, and extracting by using a water extraction method to obtain the rhodiola rosea extract in the raw materials.
5. The preparation technology of the novel anti-aging essence combining stem cell factor and plant protein as claimed in claim 2, is characterized in that: and e, soaking the fresh wolfberry fruits in low-concentration soda lye at the temperature of 60 ℃ for 1-2 min, fishing out, placing in a dish, washing with water, airing, drying in the sun or baking till the fruits are dry, and mashing to obtain the wolfberry fruits in the raw materials.
6. The preparation technology of the novel anti-aging essence combining stem cell factor and plant protein as claimed in claim 2, is characterized in that: and e, picking dry mature pericarp of the rutaceae plant and the cultivar thereof, peeling the pericarp, removing impurities, spraying water, moistening, shredding, drying, adding 50 times of water by weight, leaching for 45min at 45 ℃ in a water bath, sterilizing at 9 ℃, and continuously preserving heat and leaching for 60min to obtain the dried orange peel in the raw material.
7. The preparation technology of the novel anti-aging essence combining stem cell factor and plant protein as claimed in claim 2, is characterized in that: and e, placing the honey in a pot, heating to boil, keeping slight boiling by using slow fire, fishing out foams and waxiness floating on the surface, filtering out impurities by using gauze or gauze, diluting with boiled water, adding the diluted honey into astragalus slices with the diameter of 2.5-3.5cm, the thickness of 0.2-0.4cm and light brown or tan outer skin, uniformly stirring, moistening for 4-6h, placing the astragalus slices in a frying pot, frying by using slow fire until the roasted astragalus is deep yellow and uniform in color and luster, taking out, cooling, and extracting the cooled fried astragalus slices by using a supercritical extraction method to obtain the astragalus membranaceus in the raw materials.
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| CN113559050A (en) * | 2021-08-30 | 2021-10-29 | 上海华颜医药科技有限公司 | Preparation method of stem cell anti-aging reagent |
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| CN104546639A (en) * | 2014-12-12 | 2015-04-29 | 黄宗堂 | Stem cell essence and preparation method thereof |
| CN109157501A (en) * | 2018-09-25 | 2019-01-08 | 深圳市宝迪生物工程有限公司 | A kind of composition of the anti-aging of the extract containing stem cell and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104546639A (en) * | 2014-12-12 | 2015-04-29 | 黄宗堂 | Stem cell essence and preparation method thereof |
| CN109157501A (en) * | 2018-09-25 | 2019-01-08 | 深圳市宝迪生物工程有限公司 | A kind of composition of the anti-aging of the extract containing stem cell and application |
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| CN113559050A (en) * | 2021-08-30 | 2021-10-29 | 上海华颜医药科技有限公司 | Preparation method of stem cell anti-aging reagent |
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