CN107279639A - A kind of black tiger health-care composite beverage - Google Patents
A kind of black tiger health-care composite beverage Download PDFInfo
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- CN107279639A CN107279639A CN201710546578.0A CN201710546578A CN107279639A CN 107279639 A CN107279639 A CN 107279639A CN 201710546578 A CN201710546578 A CN 201710546578A CN 107279639 A CN107279639 A CN 107279639A
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- black tiger
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- 241000282376 Panthera tigris Species 0.000 title claims abstract description 78
- 235000013361 beverage Nutrition 0.000 title claims abstract description 22
- 239000002131 composite material Substances 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims abstract description 25
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims abstract description 25
- 235000021014 blueberries Nutrition 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 23
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000049 pigment Substances 0.000 claims abstract description 17
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- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 16
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- 238000002360 preparation method Methods 0.000 claims abstract description 9
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/44—Preservation of non-alcoholic beverages by adding preservatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/58—Colouring agents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
Abstract
The present invention provides a kind of preparation method of black tiger health-care composite beverage, and black tiger and blueberry fresh fruit are smashed to pieces after cleaning remove seed, and ethanol is extracted, and is digested, fermentation, and sterilize to obtain composite beverage.By ultrasonication Saccharomyces cerevisiae, make Saccharomyces cerevisiae phage surface formation aperture, both can quickly suppress Saccharomyces cerevisiae and carry out anaerobic fermentation, Saccharomyces cerevisiae thalline self-dissolving can be made during lactobacter growth (higher temperature and acidity increase) again.Antioxidant pigment and Polyose extraction are added directly into beverage again, effectively prevented during the fermentation, the destruction of lactic acid bacteria and saccharomycete to pigment and polysaccharide at utmost retains the antioxidation of phytochrome.Meanwhile, the natural anthocyanidin content of finished beverage is effectively increased, natural health effect that blueberry and black tiger have in itself is farthest remained.
Description
Technical field
The invention belongs to the technical field of food processing, it is related to a kind of production technology of black tiger composite beverage, more specifically
Ground, which is said, is related to a kind of preparation method for carrying out health-care composite beverage with mulberry leaf using black tiger and blueberry fresh fruit.
Background technology
Black tiger be called mountain breeze, dust storm rattan, Chinese hydrangeavine root-bark, thoroughly like a chain of pearls or a string of beads, 300 liang of silver, red brill, tonkin pepper herb, enter ground musk deer
Perfume, Mi Duoluo, wammel, orangutan kadsura longepedunculata, ZUANGUFENG, it is infrared disappear, cross bee saage, the thick leaf fruit of Chinese magnoliavine, be it is evergreen climb up by holding on to liana, it is long
3-6m.Its edible fruit is used, and aggregate fruit is subsphaeroidal, red when ripe or black purple, diameter 6-10cm or bigger；Small berries fall ovum
Shape, up to 4cm, exocarp keratin does not show seed.Seed is red, and heart-shaped or ovate is heart-shaped.The month at florescence 5-7, fruiting period 8-10
Month.Its fruit fruit shape is peculiar, pulp is fragrant and sweet, rich in anthocyanin and polyphenol.Per 100g fresh fruits containing Zn1.04mg, Fe5.03mg,
Ca150mg、VC27.57mg, total acid 0.52%, total reducing sugar 6.16%, crude protein 4.47%, belong to edible safety fruit, nutrition with
Medical value is higher, with the intelligence development that clears away heart-fire, blood-enriching face-nourishing, anti-curing cancers effect, is steep in wine with its pericarp, color is scarlet, aromatic alcohol
Just, it is often drunk, can dark hair moisturizing, physical fitness.
Blueberry also known as blue berry, belong to Ericaceae cowberry platymiscium, fruit is in blueness, in addition to material containing abundant nutrition, also
There is anthocyanin etc. to prevent the unique activity materials such as encephalasthenia, enhancing cardiac function, improving eyesight and anticancer, thus it is international
Food and agricultural organization is classified as one of big health food of the mankind five.Current China northeast, East China, Central China, South China start extensive kind
Plant suitable blueberry kind, Blueberry color is generally milky or pink, urceolus or bell, sustainable one month of florescence with
On.Anthocyanidin in Blueberry can promote the visual purple in retina cell to regenerate, pre- myopia prevention, promote eyesight.Blueberry
Fruit can play a part of adjusting blood glucose, expand patient vessel, so as to reduce blood pressure, cardioprotection, kidney prevent diabetes
Complication.Blueberry contains a large amount of physiological activators, is referred to as in fruits and vegetables " number one antioxidant ", Blueberry can be protected
Protect cell, it is to avoid destroyed by peroxide, Blueberry, which has, to be prevented functional disturbance, improve short-term memory, improve the elderly
Balance and the effect such as harmony.Anthocyanidin in Blueberry can remove free radical harmful in vivo, prevent wrinkle of skin
Generate ahead of time, reduce skin disease and ripple, the anthocyanidin of Blueberry even eliminates scar, dispelling stain, skin whitening, and
And make skin smooth for a long time, high resilience.Blueberry can also delay cranial nerve aging, the effect for improving memory, reinforce
Blood vessel, improvement circulation, improvement sleep, delay memory loss, prevention heart disease, pre- anti-cancer.
China's mulberry tree species, kind and yield are ranked the first in the world, in addition to Qinghai-Tibet Platean, and there is cultivation all parts of the country
Training.Current most area is by Sang Zuowei Silage cultivations, and every year in addition to raising silkworm, many positions of mulberry tree all do not obtain profit
With.Although many researchs are it has been reported that mulberry has multiple pharmacological effect, the research of anti-allergic effects is also rarely reported.
Publication No. CN105533746A Chinese patent discloses a kind of preparation method of black tiger instant powder, including with
Lower preparation process：1) take that 80~120 parts by weight do black tiger and 15~30 parts by weight Chinese medicine additives are cleaned respectively, crush；2)
Take half weight to do black tiger to be well mixed with Chinese medicine additive, add the yeast of Chinese medicine additive weight 0.04%~0.08%
Powder and dry black 2~5 times of water of tiger gross weight, stir, are fermented 3~7 days at 20~33 DEG C, obtain fermentate；3) to surplus
Under half weight do black tiger and add and do black 4~6 times of water of tiger gross weight, regulation pH value to 3.5~4.5, temperature is 45~
55 DEG C, the complex enzyme of dry black tiger gross weight 0.3%~0.6% is added, digests 4~7 hours, obtains zymolyte；4) will step
The rapid fermentate 2) obtained and step 3) obtained zymolyte mixes, and homogeneous is dried, obtains black tiger instant powder；Step 3) institute
The complex enzyme stated is that weight ratio is 2:7 pectin lyase and the mixture of cellulase；Described Chinese medicine additive, by following
The raw material of parts by weight is made：40~60 parts of Asian puccoon, 30~50 parts of luffa, 20~40 parts of butterflybush flower, 10~30 parts of Chinese lobelia, soil
5~15 parts of Poria cocos.This method is main to coordinate fermentation with black tiger medicinal part and other Chinese herbal medicines, to obtain Chinese medicine powder, application
Scope is not wide.
Publication No. CN105441268A Chinese patent discloses a kind of brewing method of black tiger Fermented Fruit Wine, bag
Include the following steps：Black tiger fruit is broken into slurry, magma is obtained；By the magma, water and sucrose with 1:2‐4:1-1.5 weight
Than mixing, pH value is adjusted to 3.4-4.3, obtains treating zymotic fluid；Treat to add ferment in zymotic fluid to described by 3-10% weight ratio
Female activating solution, fermentation, then sequentially pass through remove slag, ageing, obtain finished product.This method easily causes the broken of polyphenoils in black tiger fruit
Bad, along with non-remove seed, the easy browning of zymotic fluid, influence is outer existing.
Therefore, we are using black tiger, blueberry fresh fruit and mulberry leaf as primary raw material, added by certain formula, processing technology
Work, prevents color and luster brown stain and sedimentation problem, it is ensured that the antioxidation of product, then its mixture is modulated, and is advised greatly
Mould prepares good mouthfeel, nutritious Wine.
The content of the invention
Present invention aim to provide that a kind of method is simple, cost is low, with anti-oxidation function, it is easy to industrial metaplasia
Production, products obtained therefrom contains natural black tiger and Blueberry pigment, in good taste, nutritious composite beverage preparation method.
The microorganism fungus kind that the present invention is used, lactic acid bacteria is lactobacillus plantarum (Lactobacillus plantarum)
From China Committee for Culture Collection of Microorganisms's common micro-organisms center, numbering is respectively CGMCC No.1.1856.
Therefore, first purpose of the invention is to provide a kind of preparation method of black tiger health-care composite beverage, step bag
Include：
(1) black tiger pomace is mixed with a certain amount of mulberry leaf powder, after the weight water such as addition, adds pectase and be stirred
Uniformly, it is 2~5 to adjust pH, digests 2~5h, is put into fermentation tank, obtains enzymolysis liquid.
(2) 3~8% sucrose are added into enzymolysis liquid, 121 DEG C of 15~25min of wet sterilization, it is directly added into 5 after cooling~
10% Saccharomyces cerevisiae, 36~72h of aerobic fermentation terminates fermentation, then 20~45min of ultrasonication, obtains treatment fluid.
(3) 8~15% lactic acid bacterias are added into treatment fluid immediately, sealing carries out anaerobic fermentation, control hair after stirring
Ferment temperature is 35~39 DEG C, and fermentation time is 3~6 days, obtains zymotic fluid.
(4) by after zymotic fluid filtered through gauze, then filtered through microporous filter, obtain black tiger feed liquid.
(5) black tiger feed liquid, water, Caulis Kadsurae Coccineae extract, sodium carboxymethylcellulose are well mixed by a certain percentage, obtained
Black tiger health-care composite beverage.
(6) the black tiger health-care composite beverage after allotment is carried out to filling, sterilization, black tiger health-care composite beverage production is produced
Preserved under product, normal temperature.
The weight ratio of black tiger pomace described in above-mentioned steps (1) and mulberry leaf powder is 1~4kg：1kg.
Pectase usage amount described in above-mentioned steps (1) is the black tiger pomaces of 100~500g/100kg, is digested at room temperature
2~5h, wherein 1g pectinase activities requirement can buy from market purchasing and obtain in more than 3000IU.
% described in above-mentioned steps refers to solid masses kg and liquor capacity L ratios.
Filtered through gauze described in above-mentioned steps (4) refers to 2~3 layers of filtered through gauze, and the pore size filter of microporous filter is 0.6
~1.0 μm.
Certain proportion described in above-mentioned steps (5) refer to black 40~80 parts of tiger feed liquid, 10~20 parts of Caulis Kadsurae Coccineae extract,
5~10 parts of xylitol, 0.2~0.5 part of sodium carboxymethylcellulose.
Sterilize and refer to described in above-mentioned steps (6)：Directly use and be heated to 121 DEG C, and keep 10~20s to be sterilized.
Above-mentioned Saccharomyces cerevisiae powder can directly buy acquisition, such as Angel Yeast, can also pass through conventional bread microzyme
Cultural method is obtained.
Above-mentioned lactic acid bacteria can directly buy acquisition, can also obtain bacterium solution by conventional lactic acid bacteria cultural method.
Second object of the present invention is to provide the preparation method of above-mentioned Caulis Kadsurae Coccineae extract and black tiger pomace, and its is specific
Step is as follows：
I is selected without rotten black tiger fresh fruit and blueberry fresh fruit, after impurity elimination remove seed is cleaned, is smashed to pieces, obtained with bruisher
Black tiger pulp, adds a certain amount of ethanol solution, and with 0.1mol/L salt acid for adjusting pH value to 2.5~5.5, leaching is stirred at room temperature
36h~60h is carried, filters, obtains filtrate and filter residue.
II filtrate carries out being concentrated under reduced pressure into the 1/30~1/50 of original volume below 50 DEG C, and upper LSA-7 macroreticular resins are inhaled
It is attached, gradient elution is carried out with 65~85% ethanol waters of 8 times to 20 times column volumes, eluent is collected to obtain.
III eluent is concentrated under reduced pressure into dry below 50 DEG C, obtains black tiger pigment.
IV filter residue adds water, stirs 30~60min, and filtering obtains filtrate and black tiger pomace is standby, filtrate thin film evaporation
Device is concentrated to dryness, and obtains dried object, wherein volume L and filter residue quality the kg ratio for adding water are 2~4:1.
V dried object is mixed with black tiger pigment, obtains Caulis Kadsurae Coccineae extract, wherein dried object and black tiger pigment quality kg
Than for 1：2~4.
The ratio of black tiger fruit and blueberry fresh fruit is 2~5 described in above-mentioned steps I:1.
Volume L and black tiger pulp that ethanol solution is added described in above-mentioned steps I quality kg ratios are 3~6:1；Wherein
Ethanol solution refers to 75~85% ethanol solutions.
The compound method of above-mentioned 75% ethanol solution is：75mL ethanol is mixed after adding 25mL water.
Filtering refers to 2~3 layers of filtered through gauze described in above-mentioned steps I, and ceramic membrane filter is once.
A times column volume is represented with BV described in above-mentioned steps II.
Technique effect
1st, by ultrasonication Saccharomyces cerevisiae, make Saccharomyces cerevisiae phage surface formation aperture, both can quickly suppress face
Bag yeast carries out anaerobic fermentation, can make Saccharomyces cerevisiae thalline certainly during lactobacter growth (higher temperature and acidity increase) again
It is molten.
2nd, antioxidant pigment and Polyose extraction are added directly into beverage again, effectively prevented during the fermentation, breast
The destruction of sour bacterium and saccharomycete to pigment and polysaccharide, at utmost retains the antioxidation of phytochrome.Meanwhile, effectively improve
The natural anthocyanidin content of finished beverage, farthest remains the natural health work(that blueberry and black tiger have in itself
Effect.
3rd,, can be effective the characteristics of using lactic acid is produced using the pomace after pigment is extracted through lactic acid bacteria and saccharomycetes to make fermentation
Ground extends black tiger pigment and Blueberry pigment holding time, is also beneficial to improve the mouthfeel of the beverage.
4th, the health drink both make use of Blueberry pigment to improve eyesight and oxidation resistance, and hypoglycemic in mulberry leaf is made full use of again
Class material, is allowed to more be suitable for diabetes patients.
Embodiment
Below, the present invention will be further detailed with embodiment, but it is not limited to any of these embodiments
Individual or similar example.
Embodiment one
Select and cleaned without the rotten black tiger fresh fruits of 200kg and 50kg blueberry fresh fruits raw material, smashed to pieces and beaten after remove seed
Into slurry, black tiger pulp is obtained.The black tiger pulps of 250kg are taken, 800L 80% ethanol solution is added, are adjusted with 0.1mol/L hydrochloric acid
PH value is saved to 4.5, extraction 50h is stirred at room temperature, once, ceramic membrane filter once, obtains filtrate and filter residue, gained to 2 layers of filtered through gauze
Filtrate 20L is concentrated under reduced pressure into below 50 DEG C, upper LSA-7 macroporous resin adsorptions are carried out with 15BV 75% ethanol water
Gradient elution, collects to obtain eluent, eluent is concentrated under reduced pressure into below 50 DEG C dry, obtains the black tiger pigments of 12.56kg.
50kg filter residues add 120L water, stir 30~60min, once, ceramic membrane filter once, obtains filtrate and black old to 2 layers of filtered through gauze
Brave pomace is standby, and filtrate is concentrated to dryness with thin film evaporator, obtains 6.74kg dried objects.Take 2kg dried objects and the black tiger pigments of 4kg
Mixing, obtains Caulis Kadsurae Coccineae extract.
Embodiment two
The black tiger pomace 150kg of gained in Example one, adds mulberry leaf powder 100kg and water 250kg, adds pectase
250g, stirs, and it is 3.5 to adjust pH, after room temperature enzymolysis 4h, is put into fermentation tank, obtains about 450L enzymolysis liquids.In enzymolysis liquid
22kg sucrose is added, 121 DEG C of wet sterilization 25min are directly added into 30kg Saccharomyces cerevisiaes powder (Angel Yeast), aerobic hair after cooling
Ferment 60h, terminates fermentation, then ultrasonication 40min, obtains treatment fluid, adds 50kg lactic acid bacterias into treatment fluid immediately, and stirring is equal
Sealing carries out anaerobic fermentation after even, and it is 37 DEG C to control fermentation temperature, and fermentation time is 4 days, obtains zymotic fluid.Will with 3 layers of gauze
Filtering fermentation liquor, is then filtered, as black tiger feed liquid with aperture for 0.6~1.0 μm of microporous filter.120g is black
Tiger feed liquid, 10g xylitols, 40g Caulis Kadsurae Coccineae extracts (gained in embodiment one), 0.6g stabilizer CMC, are well mixed, obtain
To black tiger solution.By black tiger solution it is filling after, be heated to 121 DEG C and keep 20s, obtain black tiger health-care composite beverage,
Preserved under normal temperature.
Embodiment three
The black tiger pomace 250kg of gained in Example one, adds mulberry leaf powder 100kg and water 350kg, adds pectase
500g, stirs, and it is 3.5 to adjust pH, after room temperature enzymolysis 4h, is put into fermentation tank, obtains about 600L enzymolysis liquids.In enzymolysis liquid
45kg white granulated sugars are added, 121 DEG C of wet sterilization 20min are directly added into 65kg Saccharomyces cerevisiaes after cooling, aerobic fermentation 48h terminates
Fermentation, then ultrasonication 40min, obtain treatment fluid, add 70kg lactic acid bacterias into treatment fluid immediately, are sealed into after stirring
Row anaerobic fermentation, it is 37 DEG C to control fermentation temperature, and fermentation time is 3 days, obtains zymotic fluid.With 3 layers of gauze by filtering fermentation liquor,
Then filtered with aperture for 0.6~1.0 μm of microporous filter, as black tiger feed liquid.By the black tiger feed liquids of 150g,
12g xylitols, 35g Caulis Kadsurae Coccineae extracts (gained in embodiment one), 0.6g stabilizer CMC, are well mixed, obtain black tiger molten
Liquid.By black tiger solution it is filling after, be heated to 121 DEG C and keep 20s, obtain under black tiger health-care composite beverage, normal temperature preserve.
Claims (10)
1. a kind of preparation method of black tiger health-care composite beverage, step includes：
(1) black tiger pomace is mixed with a certain amount of mulberry leaf powder, after the weight water such as addition, adds pectase and be stirred
Even, it is 2~5 to adjust pH, digests 2~5h, is put into fermentation tank, obtains enzymolysis liquid.
(2) 3~8% sucrose are added into enzymolysis liquid, 121 DEG C of 15~25min of wet sterilization are directly added into 5~10% after cooling
Saccharomyces cerevisiae, 36~72h of aerobic fermentation terminates fermentation, then 20~45min of ultrasonication, obtains treatment fluid.
(3) 8~15% lactic acid bacterias are added into treatment fluid immediately, sealing carries out anaerobic fermentation, control fermentation temperature after stirring
Spend for 35~39 DEG C, fermentation time is 3~6 days, obtains zymotic fluid.
(4) by after zymotic fluid filtered through gauze, then filtered through microporous filter, obtain black tiger feed liquid.
(5) black tiger feed liquid, water, Caulis Kadsurae Coccineae extract, sodium carboxymethylcellulose are well mixed by a certain percentage, obtain black old
Brave health-care composite beverage.
(6) the black tiger health-care composite beverage after allotment is carried out to filling, sterilization, black tiger health-care composite beverage product is produced,
Preserved under normal temperature.
2. the method for claim 1 wherein the weight ratio of black tiger pomace described in step (1) and mulberry leaf powder is 1~4kg：
1kg。
3. the method for claim 1 wherein pectase usage amount described in step (1) is the black tiger fruits of 100~500g/100kg
Slag, digests 2~5h, wherein 1g pectinase activities requirement is in more than 3000IU at room temperature.
4. the method for claim 1 wherein filtered through gauze refers to 2~3 layers of filtered through gauze, microporous filter described in step (4)
Pore size filter be 0.6~1.0 μm.
5. the method for claim 1 wherein certain proportion described in step (5) refers to black 40~80 parts of tiger feed liquid, black tiger
10~20 parts of extract, 5~10 parts of xylitol, 0.2~0.5 part of sodium carboxymethylcellulose.
6. the method for claim 1 wherein sterilize to refer to described in step (6)：Directly use and be heated to 121 DEG C, and keep 10
~20s is sterilized.
7. the method for claim 1~6, wherein the preparation method of the Caulis Kadsurae Coccineae extract and black tiger pomace, its specific step
It is rapid as follows：
I is selected without rotten black tiger fresh fruit and blueberry fresh fruit, after impurity elimination remove seed is cleaned, is smashed to pieces with bruisher, obtains black old
Brave pulp, adds a certain amount of ethanol solution, and with 0.1mol/L salt acid for adjusting pH value to 2.5~5.5, extraction is stirred at room temperature
36h~60h, filtering, obtains filtrate and filter residue.
II filtrate is carried out being concentrated under reduced pressure into the 1/30~1/50 of original volume below 50 DEG C, and upper LSA-7 macroporous resin adsorptions are used
8BV~20BV 65~85% ethanol waters carry out gradient elution, collect to obtain eluent.
III eluent is concentrated under reduced pressure into dry below 50 DEG C, obtains black tiger pigment.
IV filter residue adds water, stirs 30~60min, and filtering obtains filtrate and black tiger pomace is standby, filtrate is dense with thin film evaporator
It is reduced to dry, obtains dried object, wherein volume L and filter residue quality the kg ratio for adding water is 2~4:1.
V dried object is mixed with black tiger pigment, obtains Caulis Kadsurae Coccineae extract, and wherein dried object is with black tiger pigment quality kg ratios
1：2~4.
8. the method for claim 7, the ratio of black tiger fruit and blueberry fresh fruit is 2~5 wherein described in step I:1.
9. the method for claim 7, adds quality kgs of the volume L with black tiger pulp of ethanol solution wherein described in step I
Than for 3~6:1；Wherein ethanol solution refers to 75~85% ethanol solutions.
10. the method for claim 7, filtering refers to 2~3 layers of filtered through gauze wherein described in step I, and ceramic membrane filter is once.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926249A (en) * | 2017-11-27 | 2018-04-20 | 桂林斯泰硕农农业科技有限公司 | Strengthen the fertilizing method of Bu Funa rhizome anticancer functions |
| CN108041364A (en) * | 2017-12-15 | 2018-05-18 | 张国群 | A kind of production method of Bu Funa fruit drinks |
| CN112042844A (en) * | 2020-09-15 | 2020-12-08 | 中南林业科技大学 | Preparation method of black tiger enzyme beverage |
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| CN105942100A (en) * | 2016-05-26 | 2016-09-21 | 长沙湘资生物科技有限公司 | Mulberry health-care compound beverage |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105942100A (en) * | 2016-05-26 | 2016-09-21 | 长沙湘资生物科技有限公司 | Mulberry health-care compound beverage |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107926249A (en) * | 2017-11-27 | 2018-04-20 | 桂林斯泰硕农农业科技有限公司 | Strengthen the fertilizing method of Bu Funa rhizome anticancer functions |
| CN108041364A (en) * | 2017-12-15 | 2018-05-18 | 张国群 | A kind of production method of Bu Funa fruit drinks |
| CN112042844A (en) * | 2020-09-15 | 2020-12-08 | 中南林业科技大学 | Preparation method of black tiger enzyme beverage |
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