CN108977312A - A kind of distiller's yeast and its application on raspberry wine fermentation - Google Patents
A kind of distiller's yeast and its application on raspberry wine fermentation Download PDFInfo
- Publication number
- CN108977312A CN108977312A CN201811005786.0A CN201811005786A CN108977312A CN 108977312 A CN108977312 A CN 108977312A CN 201811005786 A CN201811005786 A CN 201811005786A CN 108977312 A CN108977312 A CN 108977312A
- Authority
- CN
- China
- Prior art keywords
- raspberry
- wine
- distiller
- yeast
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9068—Zingiber, e.g. garden ginger
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
- A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Medical Informatics (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Organic Chemistry (AREA)
- Diabetes (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Obesity (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Nutrition Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Emergency Medicine (AREA)
- Endocrinology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a kind of distiller's yeast and its application on raspberry wine fermentation, distiller's yeast is made of the following raw material of mass parts: 10~20 parts of Chinese yam, 5~15 parts of wheat bran, 1~5 part of raspberry seed, 2~5 parts of raspberry stem extraction, 0.01~0.05 part of zytase, 10~20 parts of Chinese herbal medicine, 0.1~0.5 part of aroma-producing yeasts, 0.01~0.05 part of aspergillus oryzae;The Chinese herbal medicine is made of 4~8 parts of 3~6 parts of 1~3 part of moringa seeds, ginger peel, the oriental dodartia herb of mass parts, 4~8 parts of sorrel;Wine of raspberry is brewed by 30~50 parts of the raspberry fruit of mass parts, 20~30 parts of rice, 10~20 parts of millet.Distiller's yeast of the invention is suitable for the brew of wine of raspberry, and the wine of raspberry nutriment brewed is effectively maintained, and wine of raspberry aroma and fruital fragrance are strong, and lovely luster, mouthfeel are tasty and refreshing soft, clear, no precipitating, and quality is high.
Description
Technical field
The present invention relates to food processing technology fields, and in particular to a kind of distiller's yeast and its application on raspberry wine fermentation.
Background technique
There are a thousands of years spirits culture in China, celebrating wedding and funeral arrangements, business activity, and visiting etc. all can not keep from drink, but tradition
Spirits culture be only defeated by that wine is lively, adds to the fun and the introduction of sad scene.Gradually intensification of the modern society with human knowledge's degree, reason
Property treat wine product and will also become cultural a part of drinking.The thought that health will be drunk as modern.Therefore a kind of in good taste, battalion
Supporting abundant fruit wine is just worth that people is allowed to expect.
With the development of society, scientific and technological progress, the rhythm of people's life and work is getting faster, life stress and work
Many human bodies are allowed to be chronically at sub-health state as pressure.State has more been deepened in environmental problem, operating pressure and drug abuse
People's health problem becomes increasingly conspicuous.What Chinese ancestor initiated is to mend with food, is strengthened the body resistance to consolidate the constitution, to defend the Chinese medicine of disease to learn to read and write.Tree
Certain kind of berries fruit itself contains much beneficial to human body vitamin and plant acid, and in each stage of human evolution, raspberry is feeding
A kind of wild fruit exist.With the help of modem technology, it was recognized that raspberry fruit nutrition, therefore raspberry fruit
The production and processing of product has been brought into schedule.
Contain a large amount of injection Vitamin B_6, superoxide dismutase in raspberry fruit, Y- pacify the anti-ageing substance such as base butyric acid and
The cancer-resisting substances such as ellagic acid, wherein vitamin E and ellagic acid content be fruit most, be made into be brewed into wine of raspberry can be preferable
Preservation raspberry in nutriment, nutritive value is good, health-care effect.However, in the prior art, there are also processing raspberry fruits
The method of wine, but since fermentation condition and distiller's yeast could not be precisely controlled, lead to superoxide dismutase therein (SOD), flower
Green element, ellagic acid, total phenol and vitamin C are largely lost, and cause the nutritive value of raspberry fruit wine and health-care effect unobvious.
Summary of the invention
The present invention overcomes distiller's yeast in the prior art is single, obtained wine of raspberry is brewed in the brewing for being applied to wine of raspberry
Flavor, shade deviation, nutriment cannot be saved preferably, and wine of raspberry is also easy to produce the technical problems such as sediment, and providing can be a kind of
Distiller's yeast and its application on raspberry wine fermentation.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of distiller's yeast is made of the following raw material of mass parts: 10~20 parts of Chinese yam, 5~15 parts of wheat bran, raspberry seed 1~5
Part, 2~5 parts of raspberry stem extraction, 0.01~0.05 part of zytase, 10~20 parts of Chinese herbal medicine, 0.1~0.5 part of aroma-producing yeasts,
0.01~0.05 part of aspergillus oryzae;The Chinese herbal medicine by mass parts 3~6 parts of 1~3 part of moringa seeds, ginger peel, oriental dodartia herb 4~8
Part, 4~8 parts of sorrel compositions;
The distiller's yeast is prepared by the following method: raspberry seed, ginger peel, moringa seeds toast to 1 at 40~60 DEG C~
It is ground into powder after 2h, filters to take to obtain filtrate and filter residue after oriental dodartia herb and sorrel are decocted, filter residue and drying is crushed into powder
End;After Chinese yam boiling, it is dried, crushed into Chinese yam powder;After above-mentioned powder is mixed with zytase with oriental dodartia herb and the palm leaf
The decoction filtrate of rheum officinale soaks 2~4h of matter, pinches into distiller's yeast group;Aroma-producing yeasts are inoculated in distiller's yeast group, aspergillus oryzae is placed on 30~35
When culture to distiller's yeast group color is yellowish-brown at DEG C, continues culture to distiller's yeast in distiller's yeast group surface sprinkling raspberry stem extraction and roll into a ball face
Stop culture when color is sepia, distiller's yeast group is dried, distiller's yeast is made.
Wherein, the raspberry stem extraction is prepared by the following method: raspberry stem being smashed to pieces and is placed in extractant, is adopted
With ultrasonic wave assisted extraction, at 30~40 DEG C, 30~35min of extraction obtains the raspberry stem extraction;The extractant by
Mass ratio is the citric acid of 1:5:10:5, methanol, ethyl alcohol, acetone form;The solid-to-liquid ratio of the raspberry stem and extractant be 1~
5:30g/ml。
Another object of the present invention, which also resides in, provides a kind of wine of raspberry, and distiller's yeast of any of claims 1 or 2 is used to ferment
It is prepared;The wine of raspberry is fermented to obtain by the following raw material of mass parts: 30~50 parts of raspberry fruit, 20~30 parts of rice, small
10~20 parts of rice;
The fermentation process of the wine of raspberry includes the following steps:
Coloring: the raspberry fruit not ruptured completely after selection picking in 8 hours is protected from light broken after cleaning raspberry fruit
Juice is taken, juice of raspberry and rice and millet are impregnated into 5~8h, is cooked draining to be placed in pot after rice and millet taking-up;
Dress altar: the raspberry fresh fruit by the rice after cooking and millet and after cleaning is put into after mixing with the distiller's yeast to disappear
In wine jar after poison, seals wine jar within 1 hour and be placed in fermenting cellar and ferment, the wine jar altar body volume is 10L~20L;
Wine jar be all placed in after fermenting cellar fermenting cellar is carried out it is hermetically sealed;The additive amount of the distiller's yeast is that rice, millet, raspberry fresh fruit are total
The 0.1~0.5% of quality;
Fermentation: carrying out illumination observation to wine jar every seven working days, whether watches it completely into yeast-like fungi active state,
Inadequate for liveness to be moved away from fermenting cellar in time, just observation is primary when every seven work, by fermentation liquid mistake after 45~50d of fermentation
Leaching obtains filtrate, and filtrate is placed in sealing precipitating in new wine jar and obtains the wine of raspberry after 3 months.
Wherein, the Alcohol degree of the wine of raspberry is 16~18 degree.
Wherein, the sugariness of the wine of raspberry is 8~12.
Wherein, fermentation temperature is 20~35 DEG C.
Wherein, in the dress altar technique, when filling altar, in the dress altar technique, when filling altar, rice, millet and, distiller's yeast splendid attire
Amount is the 4/5 of wine jar altar body.
Wherein, the fermenting cellar carries out disinfection to fermenting cellar before wine jar is placed in fermenting cellar;Wine jar is put into fermentation
Time-labeling is done to wine jar behind room, wine jar is all placed in after fermenting cellar again hermetically sealed to fermenting cellar progress.When being done on wine jar
Between mark, in order to control to wine jar illumination observation.
Oriental dodartia herb is sweet, cool, nontoxic.Large and small Ji has clearing heat and detoxicating, anti-inflammatory, and hemostasis and recovery liver function promote liver thin
The regenerated effect of born of the same parents is suitable for the inflammatory bleeding of acute fever, yellow subcutaneous ulcer, hepatitis, heat gonorrhea, hematuria etc..Containing volatile oil, alkaloid,
Resin, synanthrin, cyanogenetic glycosides, saponin.
Ginger peel matter is soft, there is special aroma, and taste is peppery.There is the effect of row water, detumescence.Control edema swelling.
Moringa seeds total sugar content is 6.9% to 9.5%, and protein content is 36.2% to 38.7%, and fat content is
33.8% to 38.2%, the content of potassium is 515.3 to 617.6mg/100g, and calcium content is 95 to 135.3mg/100g, iron content
It is 2.9 to 3.5mg/100g.Wherein, oleic acid content 22.6%, vaccenic acid 18.1%, elaidic acid 13.5%, glycerol list
Oleate is 9.2%, palmitinic acid 8.1%.
Sorrel bitter, it is cold in nature, lower promoting is rushed down, purging fire and cooling blood, the row stasis of blood is broken to be accumulated, clearing heat and detoxicating.Rhizome containing fear quinones at
Divide 1.01%~5.19%, tannin 5% includes galloyl glucose, mono- catechin of d, gallic acid and tetrarin,
Chinese yam have nourish it is strong, it is aid digestion, hold back abnormal sweating, the effect of antidiarrheal, cure mainly insufficiency of the spleen diarrhoea, cough due to deficiency of the lung, glycosuria
Disease quenches one's thirst, oliguria frequency, spermatorrhea, women's leukorrhagia and dyspeptic chronic enteritis.
The ellagic acid content contained in raspberry stem extraction of the present invention is not less than 600.00ug/g and polyphenol content
16.00mg/g。
The present invention has the advantages that compared with prior art
(1) distiller's yeast of the invention uses aroma-producing yeasts and aspergillus oryzae, cooperate can by juice of raspberry and rice and
Starch, polysaccharide fermentation in millet generate ethyl alcohol, polyalcohol, organic acid and haematochrome, the raspberry fruit fruit wine phase being prepared
Than in bright in colour using saccharomyces cerevisiae brewing.Containing mucus protein and starch containing Chinese yam in the raw material of distiller's yeast of the invention can make
To contain volatile oil and unsaturated fatty acid and dimension life in the fermentation substrate of aroma-producing yeasts and aspergillus oryzae, raspberry seed and moringa seeds
The Multiple components such as plain E, raspberry seed and moringa seeds after baking can promote the generation and exudation of volatile oil, can mention when mixing with wheat bran
The adhesiveness of high wheat bran, distiller's yeast good water-retaining property, meanwhile, volatile oil can also cover ginger peel bad smell.It is poly- that wood is added in the present invention
Carbohydrase can be by the fiber degradation in raspberry seed, moringa seeds, wheat bran and ginger peel, oriental dodartia herb, sorrel at small-molecule substance, will
It is applied to raspberry wine fermentation, and wine of raspberry, which does not have to filtering, can have preferable transparency, no precipitating, and raspberry seed and moringa seeds
Raspberry seed, the active material dissolution rate in moringa seeds are high after the degradation of zytase.When prepared by distiller's yeast of the present invention, work as distiller's yeast
When for yellowish-brown, aroma-producing yeasts and aspergillus oryzae growth are vigorous, continue to ferment after spraying raspberry stem extraction, in raspberry extract
Ellagic acid and polyphenol substance can protect the color of distiller's yeast inside distiller's yeast surface penetration to distiller's yeast, meanwhile, when distiller's yeast stores, tree
Certain kind of berries extract can prevent other miscellaneous bacterias growth and breeding on distiller's yeast, and distiller's yeast is high-quality, be applied to the fermentation of wine of raspberry, raspberry
Wine quality is good.
(2) wine of raspberry is made using distiller's yeast of the invention, and uses wine of raspberry brewing method of the invention, nutrients quality guarantee
Deposit that effect is good, the nutrient substances such as vitamin, superoxide dismutase, ellagic acid, anthocyanidin of raspberry retention is good.
(3) wine of raspberry of the invention has lipid-loweringing blood sugar decreasing effect, and nutritive value is high, good health care effect.In moringa seeds
Unsaturated fatty acid and its raspberry seed Flavonoid substances can accelerate toxin expelling, boost metabolism, and oriental dodartia herb is clearing heat and detoxicating, oriental dodartia herb
Line water detumescence, aid digestion containing mucus protein in Chinese yam, the ellagic acid in raspberry extract contain anthraquinone-derivative in sorrel
Object promotees bile, digestive juice secretion, promotes digestion.The anti-oxidant work of ellagic acid and volatile oil, polyphenol substance in raspberry extract
Property strong, injection Vitamin B_6 rich in raspberry fruit, superoxide dismutase, Y- pacifies anti-ageing substance and the tan such as base butyric acid
The cancer-resisting substances such as acid, open stomach at millet qi-restoratives damage.Millet, moringa seeds, oriental dodartia herb, oriental dodartia herb, Chinese yam, raspberry extract, palm leaf
Nutriment mutual cooperation in rheum officinale and raspberry fruit can be clearing heat and detoxicating, promotes the secretion of digestive juice, accelerates toxin expelling and digestion
Cooperate flavones, the organic acid, polyphenol substance in fruit wine, can be improved the immunity of human body, remove interior free yl, reduce blood lipid,
Blood pressure, weight-reducing adjust stomach, health-care effect.
(4) wine of raspberry that is prepared of the present invention, color is ruddy, sweet and sour taste, wine of raspberry can have without filtering compared with
Transparency well, wine body are plentiful, clear, no precipitating.
(5) juice of raspberry is impregnated rice and millet by the present invention, compared to be soaked in water rice and millet is adopted, using certain kind of berries fruit
The wine of raspberry color that juice impregnates brewing is more bright-coloured, and fragrance is more rich.Wine of raspberry of the invention is sent out using raspberry and rice, millet
Ferment, rice, millet and wine of raspberry cooperate, the wine of raspberry that can not have to add sugar brewing into raspberry fruit be have plentiful wine body and
Strong wine flavour.The deficiency for compensating for sugar in raspberry fruit influences the fermentation of wine of raspberry.
(6) the wine jar tank body volume capacity that the present invention selects is within 10L-20L, container when being conducive to control fruit fermentation
Interior temperature, Space Thermal exchange, the raspberry fruit wine quality fermented are good.
Specific embodiment
Below with reference to embodiment and test, the invention will be further described.
Embodiment 1
A kind of distiller's yeast is made of the following raw material of mass parts: 20 parts of Chinese yam, 5 parts of wheat bran, 5 parts of raspberry seed, raspberry stem mention
Take 2 parts of object, 0.05 part of zytase, 10 parts of Chinese herbal medicine, 0.5 part of aroma-producing yeasts, 0.01 part of aspergillus oryzae;The Chinese herbal medicine is by matter
Measure 8 parts of 3 parts of 3 parts of moringa seeds, ginger peel, the oriental dodartia herb, 4 parts of sorrel compositions of part;The raspberry stem extraction is by the following method
It is prepared: raspberry stem being smashed to pieces and is placed in extractant, using ultrasonic wave assisted extraction, at 40 DEG C, extraction 30min is obtained
The raspberry stem extraction;The extractant is made of citric acid, methanol, ethyl alcohol, the acetone that mass ratio is 1:5:10:5;It is described
Raspberry stem and extractant solid-to-liquid ratio be 5:30g/ml.
The distiller's yeast is prepared by the following method: crushing after raspberry seed, ginger peel, moringa seeds are toasted 2h at 40 DEG C
At powder, filtrate and filter residue are filtered to take to obtain after oriental dodartia herb and sorrel are decocted, filter residue and drying is ground into powder;By Chinese yam
After boiling, it is dried, crushed into Chinese yam powder;After above-mentioned powder is mixed with zytase with the decoction of oriental dodartia herb and sorrel
Filtrate soaks matter 2h, pinches into distiller's yeast group;Culture to distiller's yeast rolls into a ball face at inoculation aroma-producing yeasts, aspergillus oryzae are placed on 35 DEG C in distiller's yeast group
When color is yellowish-brown, stop training when it is sepia that distiller's yeast group surface sprinkling raspberry stem extraction, which continues to cultivate to distiller's yeast group color,
It supports, distiller's yeast group is dried, distiller's yeast is made.
A kind of wine of raspberry uses above-mentioned distiller's yeast fermentation to be prepared;The wine of raspberry is sent out by the following raw material of mass parts
Ferment obtains: 30 parts of raspberry fruit, 30 parts of rice, 10 parts of millet;
The fermentation process of the wine of raspberry includes the following steps:
Coloring: the raspberry fruit not ruptured completely after selection picking in 8 hours is protected from light broken after cleaning raspberry fruit
Juice is taken, juice of raspberry and rice and millet are impregnated into 8h, is cooked draining to be placed in pot after rice and millet taking-up;
Dress altar: the raspberry fresh fruit by the rice after cooking and millet and after cleaning is put into after mixing with the distiller's yeast to disappear
In wine jar after poison, containing quantity is the 4/5 of wine jar altar body, and wine jar is sealed within 1 hour and is placed in fermenting cellar and is fermented, the wine
Altar altar body volume is 10L;The fermenting cellar carries out disinfection to fermenting cellar before wine jar is placed in fermenting cellar;Wine jar is put into
Time-labeling done to wine jar after fermenting cellar, wine jar be all placed in after fermenting cellar fermenting cellar is carried out it is hermetically sealed;The distiller's yeast adds
Dosage be rice, millet, raspberry fresh fruit gross mass 0.5%;
Fermentation: carrying out illumination observation to wine jar every seven working days, whether watches it completely into yeast-like fungi active state,
Inadequate for liveness to be moved away from fermenting cellar in time, just observation is primary when every seven work, takes filtering fermentation liquor after fermentation 50d
Filtrate is obtained, filtrate is placed in sealing precipitating in new wine jar and obtains the wine of raspberry after 3 months, the Alcohol degree of wine of raspberry is 16
Degree, sugariness 12;The fermentation temperature of the fermentation is 20 DEG C.
Embodiment 2
A kind of distiller's yeast is made of the following raw material of mass parts: 10 parts of Chinese yam, 15 parts of wheat bran, 1 part of raspberry seed, raspberry stem
5 parts of extract, 0.01 part of zytase, 20 parts of Chinese herbal medicine, 0.1 part of aroma-producing yeasts, 0.05 part of aspergillus oryzae;The Chinese herbal medicine by
4 parts of 6 parts of 1 part of moringa seeds, ginger peel, the oriental dodartia herb of mass parts, 8 parts of sorrel compositions;The raspberry stem extraction is by such as lower section
Method is prepared: raspberry stem being smashed to pieces and is placed in extractant, using ultrasonic wave assisted extraction, at 30 DEG C, extraction 35min is obtained
To the raspberry stem extraction;The extractant is made of citric acid, methanol, ethyl alcohol, the acetone that mass ratio is 1:5:10:5;Institute
The solid-to-liquid ratio of the raspberry stem and extractant stated is 1:30g/ml.
The distiller's yeast is prepared by the following method: crushing after raspberry seed, ginger peel, moringa seeds are toasted 1h at 60 DEG C
At powder, filtrate and filter residue are filtered to take to obtain after oriental dodartia herb and sorrel are decocted, filter residue and drying is ground into powder;By Chinese yam
After boiling, it is dried, crushed into Chinese yam powder;After above-mentioned powder is mixed with zytase with the decoction of oriental dodartia herb and sorrel
Filtrate soaks matter 4h, pinches into distiller's yeast group;Culture to distiller's yeast rolls into a ball face at inoculation aroma-producing yeasts, aspergillus oryzae are placed on 30 DEG C in distiller's yeast group
When color is yellowish-brown, stop training when it is sepia that distiller's yeast group surface sprinkling raspberry stem extraction, which continues to cultivate to distiller's yeast group color,
It supports, distiller's yeast group is dried, distiller's yeast is made.
A kind of wine of raspberry uses above-mentioned distiller's yeast fermentation to be prepared;The wine of raspberry is sent out by the following raw material of mass parts
Ferment obtains: 50 parts of raspberry fruit, 20 parts of rice, 20 parts of millet;
The fermentation process of the wine of raspberry includes the following steps:
Coloring: the raspberry fruit not ruptured completely after selection picking in 8 hours is protected from light broken after cleaning raspberry fruit
Juice is taken, juice of raspberry and rice and millet are impregnated into 5h, is cooked draining to be placed in pot after rice and millet taking-up;
Dress altar: the raspberry fresh fruit by the rice after cooking and millet and after cleaning is put into after mixing with the distiller's yeast to disappear
In wine jar after poison, containing quantity is the 4/5 of wine jar altar body, and wine jar is sealed within 1 hour and is placed in fermenting cellar and is fermented, the wine
Altar altar body volume is 20L;The fermenting cellar carries out disinfection to fermenting cellar before wine jar is placed in fermenting cellar;Wine jar is put into
Time-labeling done to wine jar after fermenting cellar, wine jar be all placed in after fermenting cellar fermenting cellar is carried out it is hermetically sealed;The distiller's yeast adds
Dosage be rice, millet, raspberry fresh fruit gross mass 0.1%;
Fermentation: carrying out illumination observation to wine jar every seven working days, whether watches it completely into yeast-like fungi active state,
Inadequate for liveness to be moved away from fermenting cellar in time, just observation is primary when every seven work, takes filtering fermentation liquor after fermentation 45d
Filtrate is obtained, filtrate is placed in sealing precipitating in new wine jar and obtains the wine of raspberry after 3 months, the Alcohol degree of wine of raspberry is 18
Degree, sugariness 8;The fermentation temperature of the fermentation is 35 DEG C.
Embodiment 3
A kind of distiller's yeast is made of the following raw material of mass parts: 15 parts of Chinese yam, 10 parts of wheat bran, 3 parts of raspberry seed, raspberry stem
3 parts of extract, 0.03 part of zytase, 15 parts of Chinese herbal medicine, 0.3 part of aroma-producing yeasts, 0.03 part of aspergillus oryzae;The Chinese herbal medicine by
7 parts of 5 parts of 2 parts of moringa seeds, ginger peel, the oriental dodartia herb of mass parts, 7 parts of sorrel compositions;The raspberry stem extraction is by such as lower section
Method is prepared: raspberry stem being smashed to pieces and is placed in extractant, using ultrasonic wave assisted extraction, at 35 DEG C, extraction 32min is obtained
To the raspberry stem extraction;The extractant is made of citric acid, methanol, ethyl alcohol, the acetone that mass ratio is 1:5:10:5;Institute
The solid-to-liquid ratio of the raspberry stem and extractant stated is 2:30g/ml.
The distiller's yeast is prepared by the following method: crushing after raspberry seed, ginger peel, moringa seeds are toasted 2h at 50 DEG C
At powder, filtrate and filter residue are filtered to take to obtain after oriental dodartia herb and sorrel are decocted, filter residue and drying is ground into powder;By Chinese yam
After boiling, it is dried, crushed into Chinese yam powder;After above-mentioned powder is mixed with zytase with the decoction of oriental dodartia herb and sorrel
Filtrate soaks matter 3h, pinches into distiller's yeast group;Culture to distiller's yeast rolls into a ball face at inoculation aroma-producing yeasts, aspergillus oryzae are placed on 32 DEG C in distiller's yeast group
When color is yellowish-brown, stop training when it is sepia that distiller's yeast group surface sprinkling raspberry stem extraction, which continues to cultivate to distiller's yeast group color,
It supports, distiller's yeast group is dried, distiller's yeast is made.
A kind of wine of raspberry uses above-mentioned distiller's yeast fermentation to be prepared;The wine of raspberry is sent out by the following raw material of mass parts
Ferment obtains: 40 parts of raspberry fruit, 25 parts of rice, 15 parts of millet;
The fermentation process of the wine of raspberry includes the following steps:
Coloring: the raspberry fruit not ruptured completely after selection picking in 8 hours is protected from light broken after cleaning raspberry fruit
Juice is taken, juice of raspberry and rice and millet are impregnated into 7h, is cooked draining to be placed in pot after rice and millet taking-up;
Dress altar: the raspberry fresh fruit by the rice after cooking and millet and after cleaning is put into after mixing with the distiller's yeast to disappear
In wine jar after poison, containing quantity is the 4/5 of wine jar altar body, and wine jar is sealed within 1 hour and is placed in fermenting cellar and is fermented, the wine
Altar altar body volume is 15L;The fermenting cellar carries out disinfection to fermenting cellar before wine jar is placed in fermenting cellar;Wine jar is put into
Time-labeling done to wine jar after fermenting cellar, wine jar be all placed in after fermenting cellar fermenting cellar is carried out it is hermetically sealed;The distiller's yeast adds
Dosage be rice, millet, raspberry fresh fruit gross mass 0.3%;
Fermentation: carrying out illumination observation to wine jar every seven working days, whether watches it completely into yeast-like fungi active state,
Inadequate for liveness to be moved away from fermenting cellar in time, just observation is primary when every seven work, takes filtering fermentation liquor after fermentation 48d
Filtrate is obtained, filtrate is placed in sealing precipitating in new wine jar and obtains the wine of raspberry after 3 months, the Alcohol degree of wine of raspberry is 17
Degree, sugariness 10;The fermentation temperature of the fermentation is 30 DEG C.
In order to illustrate technical effect of the invention, following control group is set:
Control group 1
Control group 1 and the distiller's yeast and wine of raspberry of embodiment 1 are essentially identical, and difference is, in the distiller's yeast of control group 1 not
Contain zytase.
Control group 2
Control group 2 and the distiller's yeast and wine of raspberry of embodiment 1 are essentially identical, and difference is, is free of in the distiller's yeast of control group 2
There is raspberry stem extraction.
Control group 3
Control group 3 and the distiller's yeast and wine of raspberry of embodiment 1 are essentially identical, and difference is, is free of in the distiller's yeast of control group 3
There are moringa seeds and raspberry seed.
Control group 4
Control group 4 and the distiller's yeast and wine of raspberry of embodiment 1 are essentially identical, and difference is, the zymophyte of control group 4 uses
Saccharomyces cerevisiae.
1, sensory test
Sensory evaluation method explanation
The sensory evaluation method of this test is using scoring experiment, it is desirable that panelist evaluates sample product in a manner of numeric scale
Matter, the method can evaluate the property of one or more indexs of one or more products, be widely used, and be usually used in evaluating new production
Product.
Utensil:
Sanitary cup;It gargles and uses pure water.
Experimental procedure:
1. before judging beginning, organoleptic indicator being described collectively and scoring system, specific sensory evaluation criteria are shown in Table 1;
2. 20 panelists of selection participate in this sensory evaluation.
Sensory evaluation criteria
Table 1
Organoleptic detection is carried out to wine of raspberry made of 1~embodiment of embodiment 3 and 1~control group of control group 4, using 20
A scorer carries out sense organ to wine of raspberry and divides equally, the average results of indices such as the following table 2.
Table 2
Detection | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group 1 | Control group 2 | Control group 3 | Control group 4 |
Fragrance | 24.2 | 24.5 | 23.9 | 20.2 | 15.7 | 18.4 | 10.6 |
Color | 23.4 | 24.6 | 24.5 | 18.7 | 16.8 | 12.9 | 12.8 |
Mouthfeel | 28.0 | 27.8 | 29.1 | 22.8 | 17.4 | 21.7 | 17.3 |
Structural state | 19.2 | 18.7 | 19.8 | 8.3 | 12.9 | 12.0 | 12.7 |
Total score | 94.8 | 95.6 | 97.3 | 70.0 | 62.8 | 65.0 | 53.4 |
By Tables 1 and 2 it is found that the wine of raspberry of 1~embodiment of embodiment 4, fragrance, color, mouthfeel, structural state sense
Sensory evaluation scores of official's scoring than 1~control group of control group 3 are high.Embodiment 1 is compared with control group 1 it is found that addition zytase
The structural state that fruit wine can be substantially improved, the raspberry fruit wine to ferment is transparent, clarifies, without precipitating.Embodiment 1 and control group 2
Compared to it is found that can substantially improve the color, smell and taste of wine of raspberry using raspberry stem extraction, raspberry stem extraction can inhibit miscellaneous in distiller's yeast
The growth of bacterium, wine of raspberry ferment effect are good.Embodiment 1 is compared with control group 3 it is found that the present invention adds moringa seeds and raspberry seed
Afterwards, the quality of distiller's yeast is good, high using its manufactured wine of raspberry quality of fermenting.The wine of raspberry of 1~embodiment of embodiment 3, fragrance,
Color, mouthfeel, the sensory evaluation scores of structural state are higher than the sensory evaluation scores of control group 4.Illustrate to brew wine of raspberry using saccharomyces cerevisiae
Compared to use aroma-producing yeasts and aspergillus oryzae, the wine of raspberry to ferment score on color, fragrance, structural state well below
The present invention.The present invention makes wine of raspberry optimum using aroma-producing yeasts and aspergillus oryzae, manufactured wine of raspberry aromatic flavour, and color is fresh
It is gorgeous, mouthfeel is tasty and refreshing soft, clear, no precipitating, quality is high.
2, physical and chemical index detects: measurement fermentation front and back, superoxide dismutase (SOD) content of fermentation material, flower in wine jar
Green cellulose content, ellagic acid content, total phenol content, Vitamin C content are separately recorded in such as the following table 3, table 4, table 5, table 6, table 7.
Table 3
Table 4
Table 5
Table 6
Total phenol (mg/L) | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group 1 | Control group 2 | Control group 3 | Control group 4 |
Before fermentation | 172.2 | 180.2 | 176.3 | 169.8 | 147.2 | 160.4 | 179.8 |
After fermentation | 211.6 | 212.8. | 210.5 | 189.6 | 108.8. | 171.0 | 165.2 |
Table 7
By the superoxide dismutase content, anthocyanidin content, ellagic acid content, total in table 3, table 4, table 5, table 6, table 7
Phenol content, Vitamin C content fermentation front and back content compare the superoxide dismutase it is found that in 1~embodiment of the embodiment of the present invention 3
The loss that 1~control group 4 compared to the control group is lost in enzyme, anthocyanidin, total phenol, ellagic acid, Vitamin C content is few, illustrates the present invention
Raspberry method for preparing medicated wine can be such that the nutriment in wine of raspberry preferably saves.Before 1~embodiment of embodiment 3 is fermented in table 6
Compare afterwards it is found that the content after total phenol fermentation is more preceding than fermentation high, illustrates that raspberry method for preparing medicated wine of the invention preferably retains always
Outside phenol, in raspberry wine fermentation preparation process, it can ferment and generate the content that phenolic substances improves phenolic substances in fruit wine.By 5 He of table
Embodiment 1 and the control group 2 of table 6 are it is found that the raspberry stem extraction of addition can increase containing for ellagic acid and phenolic substances in distiller's yeast
Amount, can increase the content of ellagic acid and phenolic substances in fermentation material in wine jar.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the patents of the present invention.
Claims (8)
1. a kind of distiller's yeast, which is characterized in that it is made of the following raw material of mass parts: 10~20 parts of Chinese yam, 5~15 parts of wheat bran,
1~5 part of raspberry seed, 2~5 parts of raspberry stem extraction, 0.01~0.05 part of zytase, 10~20 parts of Chinese herbal medicine, aroma-producing yeasts
0.1~0.5 part, 0.01~0.05 part of aspergillus oryzae;The Chinese herbal medicine by mass parts 3~6 parts of 1~3 part of moringa seeds, ginger peel,
4~8 parts of oriental dodartia herb, 4~8 parts of sorrel compositions;
The distiller's yeast is prepared by the following method: after raspberry seed, ginger peel, moringa seeds are toasted 1~2h at 40~60 DEG C
It is ground into powder, filtrate and filter residue is filtered to take to obtain after oriental dodartia herb and sorrel are decocted, filter residue and drying is ground into powder;It will
After Chinese yam boiling, it is dried, crushed into Chinese yam powder;After above-mentioned powder is mixed with zytase with oriental dodartia herb and sorrel
It decocts filtrate and soaks 2~4h of matter, pinch into distiller's yeast group;Inoculation aroma-producing yeasts, aspergillus oryzae are trained at being placed on 30~35 DEG C in distiller's yeast group
Support to distiller's yeast group color be yellowish-brown when, distiller's yeast group surface sprinkling raspberry stem extraction continue culture to distiller's yeast group color for palm fibre
Stop culture when brown, distiller's yeast group is dried, distiller's yeast is made.
2. a kind of distiller's yeast according to claim 1, which is characterized in that the raspberry stem extraction is prepared by the following method
To: raspberry stem is smashed to pieces and is placed in extractant, using ultrasonic wave assisted extraction, at 30~40 DEG C, 30~35min of extraction is obtained
To the raspberry stem extraction;The extractant is made of citric acid, methanol, ethyl alcohol, the acetone that mass ratio is 1:5:10:5;Institute
The solid-to-liquid ratio of the raspberry stem and extractant stated is 1~5:30g/ml.
3. a kind of wine of raspberry, which is characterized in that it uses distiller's yeast fermentation of any of claims 1 or 2 to be prepared;The raspberry
Wine is fermented to obtain by the following raw material of mass parts: 30~50 parts of raspberry fruit, 20~30 parts of rice, 10~20 parts of millet;
The fermentation process of the wine of raspberry includes the following steps:
Coloring: the completely raspberry fruit that does not rupture after selection picking in 8 hours, after raspberry fruit is cleaned, be protected from light it is broken take juice,
Juice of raspberry and rice and millet are impregnated into 5~8h, cooked draining to be placed in pot after rice and millet taking-up;
Dress altar: after raspberry fresh fruit and the distiller's yeast by the rice after cooking and millet and after cleaning are put into disinfection after mixing
Wine jar in, seal wine jar within 1 hour and be placed in fermenting cellar and ferment, the wine jar altar body volume is 10L~20L;Wine jar
All be placed in after fermenting cellar fermenting cellar is carried out it is hermetically sealed;The additive amount of the distiller's yeast is rice, millet, raspberry fresh fruit gross mass
0.1~0.5%;
Fermentation: carrying out illumination observation to wine jar every seven working days, whether watches it completely into yeast-like fungi active state, for
Liveness it is inadequate be moved away from fermenting cellar in time, just observation is primary when every seven work, takes filtering fermentation liquor after 45~50d of fermentation
Filtrate is obtained, filtrate is placed in sealing precipitating in new wine jar and obtains the wine of raspberry after 3 months.
4. a kind of wine of raspberry according to claim 3, which is characterized in that the Alcohol degree of the wine of raspberry is 16~18
Degree.
5. a kind of wine of raspberry according to claim 3, which is characterized in that the sugariness of the wine of raspberry is 8~12.
6. a kind of wine of raspberry according to claim 3, which is characterized in that in the zymotechnique, fermentation temperature be 20~
35℃。
7. a kind of wine of raspberry according to claim 3, which is characterized in that rice, small when filling altar in the dress altar technique
Rice and 4/5 that, distiller's yeast containing quantity is wine jar altar body.
8. a kind of wine of raspberry according to claim 3, which is characterized in that the fermenting cellar by wine jar be placed in fermenting cellar it
Before, it carries out disinfection to fermenting cellar;Wine jar is put into after fermenting cellar, time-labeling is done to wine jar, wine jar is all placed in after fermenting cellar again
Fermenting cellar is carried out hermetically sealed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811005786.0A CN108977312A (en) | 2018-08-30 | 2018-08-30 | A kind of distiller's yeast and its application on raspberry wine fermentation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811005786.0A CN108977312A (en) | 2018-08-30 | 2018-08-30 | A kind of distiller's yeast and its application on raspberry wine fermentation |
Publications (1)
Publication Number | Publication Date |
---|---|
CN108977312A true CN108977312A (en) | 2018-12-11 |
Family
ID=64548144
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811005786.0A Pending CN108977312A (en) | 2018-08-30 | 2018-08-30 | A kind of distiller's yeast and its application on raspberry wine fermentation |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108977312A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111500399A (en) * | 2020-04-26 | 2020-08-07 | 海南火龙酒业有限公司 | Fragrant and sweet pitaya wine and brewing method thereof |
CN113293076A (en) * | 2021-05-27 | 2021-08-24 | 深圳大鲸食品科技有限公司 | Liqueur and preparation method thereof |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105112217A (en) * | 2015-08-19 | 2015-12-02 | 向倩 | Production process of Chinese herbal medicine distiller's yeast |
CN106434168A (en) * | 2016-12-07 | 2017-02-22 | 白山中天生态科技有限公司 | Bramble wine making method |
KR20170031881A (en) * | 2015-09-14 | 2017-03-22 | 서울벤처대학원대학교 산학협력단 | Manufacturing method of rice fermented liquor |
CN107338149A (en) * | 2017-06-09 | 2017-11-10 | 韦朝胜 | A kind of glutinous fragrant distiller's yeast and its in preparation method |
CN107418830A (en) * | 2017-09-26 | 2017-12-01 | 袁永芬 | A kind of blueberry fruity Aloes wine and preparation method thereof |
CN107858233A (en) * | 2017-12-13 | 2018-03-30 | 柳州市象行教育科技有限责任公司 | A kind of preparation method of Banana Wine distiller's yeast |
CN108285849A (en) * | 2018-05-02 | 2018-07-17 | 蒙阴县恒东树莓研究所 | A kind of brewing method of raspberry fruit wine |
-
2018
- 2018-08-30 CN CN201811005786.0A patent/CN108977312A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105112217A (en) * | 2015-08-19 | 2015-12-02 | 向倩 | Production process of Chinese herbal medicine distiller's yeast |
KR20170031881A (en) * | 2015-09-14 | 2017-03-22 | 서울벤처대학원대학교 산학협력단 | Manufacturing method of rice fermented liquor |
CN106434168A (en) * | 2016-12-07 | 2017-02-22 | 白山中天生态科技有限公司 | Bramble wine making method |
CN107338149A (en) * | 2017-06-09 | 2017-11-10 | 韦朝胜 | A kind of glutinous fragrant distiller's yeast and its in preparation method |
CN107418830A (en) * | 2017-09-26 | 2017-12-01 | 袁永芬 | A kind of blueberry fruity Aloes wine and preparation method thereof |
CN107858233A (en) * | 2017-12-13 | 2018-03-30 | 柳州市象行教育科技有限责任公司 | A kind of preparation method of Banana Wine distiller's yeast |
CN108285849A (en) * | 2018-05-02 | 2018-07-17 | 蒙阴县恒东树莓研究所 | A kind of brewing method of raspberry fruit wine |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111500399A (en) * | 2020-04-26 | 2020-08-07 | 海南火龙酒业有限公司 | Fragrant and sweet pitaya wine and brewing method thereof |
CN113293076A (en) * | 2021-05-27 | 2021-08-24 | 深圳大鲸食品科技有限公司 | Liqueur and preparation method thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4616413B1 (en) | Fermented food manufacturing method and fermented food | |
KR101278657B1 (en) | Polygonum multiflorum for promoting hair to be black and method thereof | |
CN104877876A (en) | Mulberry black rice wine and making method thereof | |
CN109207328B (en) | Production method of green plum wine | |
CN103773702A (en) | Saccharomyces cerevisiae strain and green plum fruit wine prepared by using saccharomyces cerevisiae strain | |
CN104774700A (en) | Cherokee rose fruit craft beer and preparation method thereof | |
CN104543822A (en) | Preparation method of fermented traditional Chinese dealcoholic functional beverage | |
CN107668702A (en) | A kind of production technology of trifoliate orange Dulcis ferment | |
CN106190676A (en) | A kind of preparation method of Yam Beer | |
CN103740529B (en) | A kind of production technique of sweet osmanthus pear fruit wine | |
CN108977312A (en) | A kind of distiller's yeast and its application on raspberry wine fermentation | |
CN112048404A (en) | Trollflower-sea buckthorn compound wheat beer and brewing method thereof | |
KR101297610B1 (en) | Manufacturing method of gyrophora esculenta wine and gyrophora esculenta wine thereof | |
CN108795684A (en) | One kind is enriched blood lipid-loweringing jujube health preserving vinegar and preparation method thereof | |
CN110607218B (en) | Sobering-up vinegar and preparation method and application thereof | |
CN112500970A (en) | Preparation method of dogwood wine | |
CN108148705A (en) | A kind of brewing method of hawthorn yellow wine and the hawthorn yellow wine of high flavones content | |
CN110093239B (en) | Noni compound wine and preparation method thereof | |
CN107937185A (en) | A kind of production method of dendrobium candidum beer | |
CN112195080A (en) | Production method of mulberry brandy | |
CN109497499B (en) | Mulberry soy sauce | |
CN105820926A (en) | Production process of fermented rose wine | |
KR20190111592A (en) | Food Composition Comprising Polygonum Cuspidatum and Noble Preparation Method thereof | |
KR102346415B1 (en) | Manufacture of makgeolli using natural substances and its method | |
CN108315115A (en) | A kind of peach blossom beer and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181211 |