CN112772251A - Planting method for increasing content of tea epigallocatechin gallate - Google Patents

Planting method for increasing content of tea epigallocatechin gallate Download PDF

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Publication number
CN112772251A
CN112772251A CN202011607120.XA CN202011607120A CN112772251A CN 112772251 A CN112772251 A CN 112772251A CN 202011607120 A CN202011607120 A CN 202011607120A CN 112772251 A CN112772251 A CN 112772251A
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Prior art keywords
tea
film
planting method
content
epigallocatechin gallate
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Inventor
金晶
叶俭慧
邵静娜
闾怡清
方奇挺
叶影
姜娟萍
李婧
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ZHEJIANG AGRICULTURAL TECHNOLOGY PROMOTION CENTER
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ZHEJIANG AGRICULTURAL TECHNOLOGY PROMOTION CENTER
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth

Abstract

The invention discloses a planting method for improving the content of epigallocatechin gallate of tea leaves, which comprises the following steps: arranging a film above the surface of the tea garden tent, wherein the film is red, and the surface of the film is provided with a through hole; the film enables sunlight to form single red visible light below the film after passing through the surface of the film; the through holes enable the tea tree canopy surface to be irradiated with the constant sunlight spectrum (especially the short-wave spectrum such as ultraviolet rays). The method can be used in any time period of the tea growth stage, is simple and feasible, can be used in a large-area tea garden, and has low cost. According to the invention, the light quality irradiated on the tent surface of the tea tree is changed through the red film, and then short-wave trace light elements such as ultraviolet light and the like are provided for the growth of the tea tree through the through holes, so that the growth state and quality component synthesis of the tea tree can be effectively adjusted, and the EGCG content in the tea leaves is improved; compared with the hybrid cultivation in the prior art, the method has the advantages of short time consumption, simple process and stronger field operability.

Description

Planting method for increasing content of tea epigallocatechin gallate
Technical Field
The invention relates to the technical field of plant cultivation tests, in particular to a planting method for improving the content of epigallocatechin gallate in tea.
Background
Epigallocatechin gallate (EGCG) is the most important quality and active component in tea, and has multiple physiological effects of resisting oxidation, inflammation, bacteria and tumor. In the prior art, the method for improving the EGCG content of tea leaves is to breed a tea variety with high EGCG content by traditional crossbreeding. However, as the genetic transformation system of tea trees is not successfully established, gene editing and accurate breeding cannot be realized, and the traditional cross screening mode is still adopted for tea tree breeding. This requires long-term work accumulation and long-term observation of new varieties by tea tree breeders, and it is only possible to breed a new variety of tea trees with stable traits. The breeding of a new tea variety usually requires nearly ten years of breeding time, is time-consuming and long, and has uncertain results.
Disclosure of Invention
The invention aims to provide a planting method for improving the content of epigallocatechin gallate of tea leaves, which can solve one or more of the technical problems.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
a planting method for increasing the content of tea epigallocatechin gallate comprises the following steps: arranging a film above the surface of the tea garden tent, wherein the film is red, and the surface of the film is provided with a through hole; the film enables sunlight to form a single red dominant visible light under the film after passing through the surface of the film; the through holes enable the sunlight spectrum to be constantly irradiated on the tea tree canopy surface, particularly the spectrum of short wave such as ultraviolet rays.
The film can be completely or partially transparent and is not limited. If the light-transmitting and the light-proof are staggered, the arrangement which is staggered and uniform is the preferable scheme. The through hole may be disposed in the light-transmitting portion or the light-opaque portion, and is not limited.
In the invention, the material of the light-transmitting film is not limited, and the light-transmitting film can be plastic, knitted, organic glass and the like, the thickness of the light-transmitting film is not limited, and the light-transmitting film is generally thinner (for example, about 2 mm), so that the cost is lower, and the use is more convenient.
Generally, the red perforated film is covered before tea leaf picking, and the film is collected during or after tea leaf picking.
The invention can effectively change the light quality irradiated on the tea plant, and the visible red light becomes the main light spectrum, and the tea plant is covered for a certain time, so that the content of epigallocatechin gallate (EGCG) in the prepared green tea is obviously improved.
The method can be used in any time period of the tea growth stage, is simple and feasible, can be used in a large-area tea garden, and has low cost.
Preferably: the through holes are uniformly formed in the thin film. Can make visible light and non-visible light uniformly irradiate on the tea tree.
Preferably: the light shading rate of the film is 50-95%. Effectively improves the color of the tea leaves, makes the tea leaves greener, and can be applied to the production of matcha. Effectively improve the content of epigallocatechin gallate (EGCG) in the tea leaves and provide other micro-illumination for the growth of plants.
Preferably: the film is used at any stage of growth of the tea plant.
Preferably: the tea trees are of the age capable of picking tea. Thereby minimizing the cost of stage usage.
Preferably: the covering time of the film on the tea tree tent surface is 5-90 days. The selection is carried out according to the actual use requirement.
Preferably: the film is 0.2-3 m above the tea tree. The height is favorable for ventilation, and when the height is more than one meter, the film does not need to be removed in the tea leaf picking process.
Preferably: the membrane is open or closed at its periphery. The film is selected according to actual use requirements, and the film can be more easily operated and can be freely stored by opening the film.
Preferably: the film is a red sunshade net. The price is cheap and the product is convenient to obtain.
The invention has the technical effects that:
according to the invention, the light quality irradiated on the tent surface of the tea tree is changed through the red film, and then short-wave trace light elements such as ultraviolet light and the like are provided for the growth of the tea tree through the through holes, so that the growth state and quality component synthesis of the tea tree can be effectively adjusted, and the content of EGCG in the tea leaves is increased; compared with the hybrid cultivation in the prior art, the method has the advantages of short time consumption, simple process and stronger field operability.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
In the drawings:
FIG. 1 is a spectral inspection under a red sunshade screen and under no sunshade screen.
Detailed Description
The present invention will now be described in detail with reference to the drawings and specific embodiments, wherein the exemplary embodiments and descriptions are provided only for the purpose of illustrating the present invention and are not to be construed as unduly limiting the invention.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
A planting method for increasing the content of tea epigallocatechin gallate comprises the following steps: a film is arranged above the surface of the tea garden awning, the film is red, and the light shading rate of the film is 50% -95%. The film is used at any stage of growth of the tea plant.
The surface of the film is provided with a through hole; the through holes are uniformly formed in the thin film. The tea tree can be uniformly irradiated by visible light with red leading and ultraviolet light with short wave.
The covering time of the film on the tea tree tent surface is 5-90 days. The film is 0.2-3 m above the tea tree.
The film enables sunlight to form a single red visible light below the film after passing through the surface of the film; the through holes enable sunlight spectrum to be constantly irradiated on the tea tree tent surface.
Most preferably, the tea tree is a red sunshade net used on the surface of a tea garden shed with the age of the tea tree capable of picking tea. The film is ventilated underneath.
The red sun-shading net is visible at will, is favorable for popularization, and can effectively improve the quality of the tea. The light quality of the tent surface of the tea tree is changed, and meanwhile, short-wave ultraviolet light irradiation is provided for the growth of the tea tree, so that the healthy and high-quality growth of plants is guaranteed.
The illumination change is in a small-range laboratory or a greenhouse, and the influence of the spectrum on the growth of tea trees is researched through LED illumination with different colors. However, for the field such as a tea garden, it is impractical to change the spectrum in a large area, and the cost of manpower and material resources is high. Although in the prior art, tea growers mostly use black sunshade nets in actual production, so as to cool tea trees and avoid strong illumination; however, the idea that the illumination spectrum can be changed by changing the color of the sunshade net is unprecedented, the invention jumps out of a thinking dead zone and an inertia thinking, changes the light quality of the tea tent surface by changing the color of the sunshade net, realizes the illumination adjustment, simultaneously keeps short wave ultraviolet rays beneficial to the growth of plants, has feasible and simple operation, has low cost for the natural field of a tea garden, can be used for at least three to five years by using one red sunshade net, and only needs to replace the black sunshade net used in the prior art, can effectively improve the quality of the tea and improve the income of tea farmers.
As shown in figure 1, in the graph of the illumination intensity and the spectrum change of the red sunshade net and the sunshade net not used, the invention can realize the full-wave-band illumination of the sunlight, and can enhance the visible red light compared with the illumination of other wavelengths to adjust the growth needs of plants.
The advantages of the present invention are further explained below by using red and black sunshade nets, no sunshade net, in different tea gardens for comparative tests.
Example one
The tea variety selected here is Fuding white tea; selecting three tea gardens with the same area and the labels of S1, S2 and S3; wherein
S1 covering a red sunshade net 1.5 m above the surface of the tea garden tent, wherein the shading rate of the red sunshade net is 95%, and the treatment lasts 15 days. The red sunshade net is open at the periphery.
S2 covering the position 1.5 m above the surface of the tea garden with a black sunshade net with a shading rate of 95% for 15 days. The periphery of the red sunshade net is open;
and S3 no sunshade net is arranged above the surface of the tea garden and the treatment lasts for 15 days.
And taking the fresh leaf samples of the three tea gardens to prepare the green tea.
The results are shown in tables 1 and 2; wherein table 1 is EGCG content data and table 2 is tea color data.
Figure BDA0002872108080000041
Figure BDA0002872108080000042
In summary, in this example, the EGCG content is shown in table 1, and the EGCG content of green tea treated by red sunshade net for 15 days is increased by about 8.2% compared with that of green tea treated by non-sunshade net. Compared with a black sunshade net, the improvement is about 8.8 percent.
The color of the tea is shown in table 2, and the green tea dried tea treated by the red sunshade net for 15 days is greener than the fuding white tea without the sunshade net. Has no obvious difference with the tea made by the black sunshade net.
Example two
The tea variety selected here is Fuding white tea; selecting three tea gardens with the same area and the labels of S1, S2 and S3; wherein
S1 covering a red sunshade net 1.5 m above the surface of the tea garden tent, wherein the shading rate of the red sunshade net is 95%, and the treatment lasts 20 days. The red sunshade net is open at the periphery.
S2 covering the position 1.5 m above the surface of the tea garden with a black sunshade net with a shading rate of 95% for 20 days. The periphery of the red sunshade net is open;
and S3 no sunshade net is arranged above the surface of the tea garden and the tea garden is treated for 20 days.
And taking the fresh leaf samples of the three tea gardens to prepare the green tea.
The results are shown in tables 3 and 4; wherein table 1 is EGCG content data and table 2 is tea color data.
Figure BDA0002872108080000051
Figure BDA0002872108080000052
In summary, in this example, the EGCG content is shown in table 3, and the EGCG content of green tea treated by the red sunshade net for 20 days is increased by about 17.5% compared with that of green tea treated by the non-sunshade net. Compared with a black sunshade net, the improvement is about 37%.
The color of the tea is shown in table 4, and the green tea dried tea treated by the red sunshade net for 20 days is greener than the fuding white tea without the sunshade net. The difference with the tea made by the black sunshade net is within an acceptable range, and the method is also suitable for producing matcha.
EXAMPLE III
The tea variety selected here is Hope tea No. 1; selecting three tea gardens with the same area and the labels of S1, S2 and S3; wherein
S1 covering a red sunshade net 1.5 m above the surface of the tea garden tent, wherein the shading rate of the red sunshade net is 95%, and the treatment lasts 15 days. The red sunshade net is open at the periphery.
S2 covering the position 1.5 m above the surface of the tea garden with a black sunshade net with a shading rate of 95% for 15 days. The periphery of the red sunshade net is open;
and S3 no sunshade net is arranged above the surface of the tea garden and the treatment lasts for 15 days.
And taking the fresh leaf samples of the three tea gardens to prepare the green tea.
The results are shown in tables 5 and 6; wherein table 5 is EGCG content data and table 6 is tea color data.
Figure BDA0002872108080000061
Figure BDA0002872108080000062
In summary, in this example, the EGCG content is shown in table 5, and the EGCG content of green tea treated by red sunshade net for 15 days is increased by about 16.8% compared with that of green tea treated by non-sunshade net. Compared with a black sunshade net, the sunshade net is improved by about 11 percent.
The color of the tea is shown in table 6, and the tea of the Wanghai tea No. 1 variety is darker; therefore, compared with the Homoku No. 1 tea without the sun-shading net, the green tea dried tea treated by the red sun-shading net for 15 days has no obvious color difference. The difference with the tea made by the black sunshade net is not obvious.
Example four
The tea variety selected here is Longjing 43; selecting three tea gardens with the same area and the labels of S1, S2 and S3; wherein
S1 covering a red sunshade net 1.5 m above the surface of the tea garden tent, wherein the shading rate of the red sunshade net is 95%, and the treatment lasts 15 days. The red sunshade net is open at the periphery.
S2 covering the position 1.5 m above the surface of the tea garden with a black sunshade net with a shading rate of 95% for 15 days. The periphery of the red sunshade net is open;
and S3 no sunshade net is arranged above the surface of the tea garden and the treatment lasts for 15 days.
And taking the fresh leaf samples of the three tea gardens to prepare the green tea.
The results are shown in tables 7 and 8; wherein table 7 is EGCG content data and table 8 is tea color data.
Figure BDA0002872108080000071
Figure BDA0002872108080000072
In summary, in this example, the EGCG content is as shown in table 7, and the EGCG content of green tea treated by red sunshade net for 15 days is increased by about 18.3% compared with that of green tea treated by non-sunshade net. Compared with a black sunshade net, the improvement is about 15.6 percent.
The color of the tea is shown in table 8, and the green tea dried tea treated by the red sunshade net for 15 days is greener than the longjing 43 dried tea without the sunshade net. The difference with the tea made by the black sunshade net is not obvious.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A planting method for increasing the content of tea epigallocatechin gallate is characterized in that: the method comprises the following steps:
arranging a film above the surface of the tea garden tent, wherein the film is red, the surface of the film is provided with through holes, and the film enables sunlight to form single red dominant visible light below the film after passing through the surface of the film;
the through holes enable sunlight spectrum to be constantly irradiated on the tea tree tent surface.
2. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the through holes are uniformly formed in the thin film.
3. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the light shading rate of the film is 50-95%.
4. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the film is used at any stage of growth of the tea plant.
5. The planting method for increasing the content of epigallocatechin gallate of tea leaves as claimed in claim 4, wherein: the tea trees are of the age capable of picking tea.
6. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the covering time of the film on the tea tree tent surface is 5-90 days.
7. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the film is 0.2-3 m above the tea tree.
8. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the membrane is open at the periphery.
9. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to claim 1, wherein the planting method comprises the following steps: the membrane is closed peripherally.
10. The planting method for increasing the content of epigallocatechin gallate of tea leaves according to any one of claims 1 to 9, wherein: the film is a red sunshade net.
CN202011607120.XA 2020-12-30 2020-12-30 Planting method for increasing content of tea epigallocatechin gallate Pending CN112772251A (en)

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CN105706758A (en) * 2016-02-29 2016-06-29 福建省农业科学院土壤肥料研究所 Method for improving yield and quality of tea by means of adjusting illumination intensity
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