CN112755165A

CN112755165A - A Chinese medicinal composition for treating mental diseases, and its preparation method

Info

Publication number: CN112755165A
Application number: CN202110193223.4A
Authority: CN
Inventors: 杨雄涛
Original assignee: Beijing Gulou Institute Of Difficult Disease Medicine Co ltd
Current assignee: Beijing Gulou Institute Of Difficult Disease Medicine Co ltd
Priority date: 2021-02-20
Filing date: 2021-02-20
Publication date: 2021-05-07

Abstract

The invention provides a traditional Chinese medicine composition for treating mental diseases and a preparation method thereof, belonging to the technical field of traditional Chinese medicine compositions, and the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-10 parts of garden balsam stem, 10-13 parts of weeping forsythia, 5-12 parts of Chinese starjasmine stem, 2-5 parts of silktree albizzia bark, 10-20 parts of blood angelica, 2-5 parts of platycladi seed, 15-25 parts of clematis root, 2-5 parts of ephedra, 1-4 parts of prepared common monkshood mother root, 1-2 parts of argy wormwood leaf, 2-4 parts of ginger, 2-5 parts of groundsel, 1-3 parts of medlar, 1-2 parts of American ginseng and 1-4 parts of lycopodium clavatum. The traditional Chinese medicine compound preparation has obvious improvement effect on mental diseases such as migraine, depression and the like, has the characteristics of low toxicity and high efficiency, has the characteristics of improving symptom identification, small side effect, low afterburning rate and the like, has the characteristics of equivalent or superior effect to a positive control medicine, is prepared into a slow release preparation, and has long-acting effect and slow release effect.

Description

A Chinese medicinal composition for treating mental diseases, and its preparation method

Technical Field

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for treating mental diseases and a preparation method thereof.

Background

Mental diseases refer to clinical manifestations of disorders of mental activities such as cognition, emotion, will and behavior, caused by dysfunction of the brain under the influence of various biological, psychological and social environmental factors. Mental activities include cognitive activities consisting of sensation, perception, attention, memory, thinking, emotional activities and mental activities, which are closely related and coordinated to each other, maintaining the unity and integrity of mental activities. The most mental disease patients have prolonged course and easy recurrence of disease fluctuation, and require continuous and regular medication to control symptoms and prevent recurrence. Numerous studies indicate that improving the compliance of psychiatric patient treatments is key to controlling psychotic symptoms and improving the prognosis of psychosis. Although the new generation of antipsychotic and antidepressant drugs have been well established and less side effects have been generally welcomed, the level of mental patient's awareness and compliance with these drug therapies remains an important issue facing current psychiatric clinical treatment and care.

Migraine is a recurrent disease caused by primary intracranial vascular motor and nerve function regulation disorder, and has the characteristics of long course of disease, intermittent repeated attack and persistent and difficult healing. 2.4 million migraine sufferers worldwide suffer from 14 million migraine headaches each year. The traditional medicine of China considers that: migraine belongs to the category of "wind of head", "wind of side head" and "wind of head bias" in traditional Chinese medicine. Clematis root is a good medicine for treating headache from ancient times, and the first monograph of pharmacy in China, Shennong's herbal Jing, has the record of clematis root for treating headache.

Depression is a mental disease seriously harming human physical and mental health, the lifetime prevalence rate is 6.1-9.5%, and 15% of patients with serious depression end life due to suicide. The antidepressant drugs used clinically at present have large side effects and reduced curative effect after long-term use and are not satisfactory.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for treating mental diseases and a preparation method thereof, the traditional Chinese medicine compound preparation has obvious improvement effect on mental diseases such as migraine, depression and the like, has the characteristics of low toxicity and high efficiency, has the characteristics of improving symptom identification, small side effect, low reburning rate and the like, and is prepared into a sustained-release preparation with long-acting effect and sustained-release effect, and the effect is equal to or superior to that of a positive control medicine.

The technical scheme of the invention is realized as follows:

the invention provides a Chinese medicinal composition for treating mental diseases, which is prepared from the following raw materials in part by weight: 5-10 parts of garden balsam stem, 10-13 parts of weeping forsythia, 5-12 parts of Chinese starjasmine stem, 2-5 parts of silktree albizzia bark, 10-20 parts of blood angelica, 2-5 parts of platycladi seed, 15-25 parts of clematis root, 2-5 parts of ephedra, 1-4 parts of prepared common monkshood mother root, 1-2 parts of argy wormwood leaf, 2-4 parts of ginger, 2-5 parts of groundsel, 1-3 parts of medlar, 1-2 parts of American ginseng and 1-4 parts of lycopodium clavatum.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 6-9 parts of garden balsam stem, 11-12 parts of weeping forsythia, 6-10 parts of Chinese starjasmine stem, 3-4 parts of cortex albiziae, 12-18 parts of blood angelica, 3-4 parts of platycladi seed, 17-22 parts of clematis root, 3-4 parts of ephedra, 2-3 parts of prepared common monkshood mother root, 1.2-1.8 parts of argy wormwood leaf, 2.5-3.5 parts of ginger, 3-4 parts of groundsel, 1.5-2.5 parts of medlar, 1.2-1.9 parts of American ginseng and 2-3 parts of lycopodium clavatum.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 7 parts of garden balsam stem, 12 parts of weeping forsythia, 8 parts of Chinese starjasmine stem, 3.2 parts of silktree albizzia bark, 14 parts of blood angelica, 3.5 parts of platycladi seed, 19 parts of clematis root, 3.4 parts of ephedra, 2.8 parts of prepared common monkshood mother root, 1.4 parts of argy wormwood leaf, 2.8 parts of ginger, 3.2 parts of groundsel, 1.7 parts of medlar, 1.4 parts of American ginseng and 2.2 parts of common clubmoss herb.

As a further improvement of the invention, the health-care food is prepared from the following raw materials in parts by weight: 8 parts of garden balsam stem, 11.2 parts of weeping forsythia, 9 parts of Chinese starjasmine stem, 3.7 parts of silktree albizzia bark, 16 parts of blood Chinese angelica, 3.8 parts of platycladi seed, 20 parts of clematis root, 3.8 parts of ephedra, 2.3 parts of prepared common monkshood mother root, 1.7 parts of argy wormwood leaf, 3.2 parts of ginger, 3.6 parts of groundsel, 2.2 parts of medlar, 1.7 parts of American ginseng and 2.5 parts of lycopodium clavatum.

The invention further provides a preparation method of the traditional Chinese medicine composition for treating mental diseases, which comprises the following steps:

s1, weighing all the traditional Chinese medicine materials in proportion, respectively cleaning, drying, grinding into powder, and sieving to obtain traditional Chinese medicine powder;

s2, adding water into the Chinese medicine powder obtained in the step S1, decocting for 3-5 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the obtained filtrate under reduced pressure to relative density of 1.2-1.5 to obtain water extract, adding sodium alginate, and mixing to obtain solution A;

s3, adding an acetic acid solution into the solid obtained in the step S2, performing reflux extraction for 2-3 times, combining extracting solutions, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.0-1.3 to obtain an acid extract, adding chitosan, and uniformly mixing to obtain a solution B;

s4, preparing 5-10 wt% of calcium chloride solution to obtain a calcium chloride curing agent;

s5, adding the solution A obtained in the step S2 into the solution B obtained in the step S3, uniformly mixing, and adding the calcium chloride curing agent obtained in the step S4 into the mixed solution to form gel, so as to obtain the traditional Chinese medicine composition hydrogel for treating mental diseases.

As a further improvement of the invention, the size of the sieve in the step S1 is 100-200 meshes.

As a further improvement of the invention, the adding amount of the sodium alginate in the step S2 is 20-35 wt% of the water extract.

As a further improvement of the invention, the chitosan is added in an amount of 15-20 wt% of the acid extract in step S3.

As a further improvement of the present invention, in step S2, the solid-to-liquid ratio of the traditional Chinese medicine powder to water is 1: (10-20) g/mL.

As a further improvement of the present invention, the solid-to-liquid ratio of the solid and the acetic acid solution in step S3 is 1: (5-15) g/mL, and the mass percent concentration of the acetic acid solution is 5-15 wt%.

Speranskia herb, pungent in flavor and warm in nature. Enters liver and kidney meridians, and has the effects of dispelling wind and eliminating dampness, relaxing muscles and tendons, promoting blood circulation, removing blood stasis and swelling, and removing toxic substances and relieving pain. Can be used for treating rheumatalgia, contracture of tendons and bones, cold-dampness loempe, waist sprain, paralysis, amenorrhea, eczema of scrotum, skin ulcer, furuncle, and pyogenic infections; forsythia, bitter in taste, slightly cold in nature; enters the channels of the lung, heart and small intestine, and has the effects of clearing away heat and toxic materials, relieving swelling, resolving masses and dispelling wind and heat. Mainly treats sore, carbuncle and pyogenic infections, scrofula, subcutaneous nodule, wind-heat exogenous infection, early warm diseases, pyretic stranguria and pain; trachelospermi caulis, bitter in taste, slightly cold in nature; it has the functions of dispersing pathogenic wind, dredging meridian, cooling blood and eliminating swelling. Mainly treats rheumatism and pyretic arthralgia, pharyngitis, carbuncle and swelling, traumatic injury, wind-evil dispelling and collateral dredging, blood cooling and swelling diminishing, good wind-evil dispelling and collateral dredging, bitter taste and dampness eliminating, and mild cold heat clearing, and is particularly suitable for rheumatism and pyretic arthralgia, spasm of muscles and tendons, and soreness and pain of waist and knees; cortex albiziae, sweet in taste and mild in nature; it has effects of dispersing depressed liver-qi, tranquilizing mind, promoting blood circulation, and relieving swelling. Is mainly used for treating restlessness, irritability, melancholy, dysphoria, insomnia, traumatic injury, fracture of tendon and tendon, swelling and pain due to blood stasis, pulmonary abscess, sore and carbuncle, pyogenic infections, and the like; xue Dang Gui is bitter and sour in taste and cold in nature. Enters lung and liver meridians, and has the effects of cooling blood, stopping bleeding, clearing heat, relaxing bowels, removing toxicity and killing parasites. It is commonly used for hematemesis, hemoptysis, metrorrhagia, constipation, carbuncle, swelling, sore, scald, burn, scabies, eczema, cooling blood for hemostasis, clearing heat, relaxing bowels, removing toxic substance, and killing parasite; arborvitae seed, sweet in flavor and mild in nature; it enters heart, kidney and large intestine channels and has the functions of nourishing heart, tranquilizing, moistening intestine and relaxing bowels. Mainly treats palpitation and insomnia, constipation due to intestinal dryness, and palpitation and insomnia, and is mainly used for palpitation, severe palpitation, vexation, insomnia, dizziness, amnesia and the like caused by heart-yin deficiency, heart-blood deficiency and heart-mind malnutrition; clematis chinensis, pungent, salty and warm in nature; the traditional Chinese medicine composition enters the bladder channel, is mainly used for treating rheumatism arthralgia syndrome, bone carp throat, traumatic injury pain, headache, toothache, epigastric pain, phlegm-fluid retention, dysphagia, female abdominal mass, breast lump and the like, is used for dispelling rheumatism, dredging collaterals and relieving pain and eliminating bone sticking, and is mainly used for treating rheumatism arthralgia syndrome, bone carp throat, traumatic injury pain, headache, toothache, epigastric pain, phlegm-fluid retention, dysphagia, female abdominal mass, breast lump and the like; ephedra herb, pungent and slightly bitter in flavor, warm in nature; enters lung and bladder meridians, and has the effects of inducing sweat, relieving exterior syndrome, dispersing lung qi, relieving asthma, inducing diuresis and relieving swelling. It can be used for treating wind-cold type common cold, cough, asthma, edema, apoplexy, typhoid fever, headache, and warm malaria. Relieving exterior syndrome, promoting sweating, eliminating pathogenic heat, relieving cough, reversing the flow of qi, removing cold and heat, and eliminating mass; prepared Sichuan aconite root, radix Aconiti Preparata is pungent, bitter and hot in flavor. Heart, liver, kidney and spleen channels, and has effects of dispelling pathogenic wind, removing dampness, warming channels and relieving pain. Can be used for treating arthralgia due to wind-cold-dampness, arthralgia, hernia pain, anesthesia, pain, and tinea pedis; folium artemisiae argyi, pungent and bitter in flavor and warm in nature; enters liver, spleen and kidney meridians, and has the effects of warming meridians, stopping bleeding, dispelling cold, relieving pain, eliminating dampness and relieving itching. Can be used for treating hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, gestational hemorrhage, menoxenia, dysmenorrhea, threatened abortion, psychroalgia of heart and abdomen, diarrhea and dysentery, cholera, spasm of muscle, leukorrhagia, eczema, scabies, tinea, hemorrhoid, and carbuncle; ginger, pungent in flavor and warm in nature; enters lung, spleen and stomach meridians, and has the effects of relieving exterior syndrome, dispelling cold, warming middle energizer, relieving vomiting, warming lung, relieving cough and removing toxic substance. Can be used for treating wind-cold type common cold, spleen and stomach cold syndrome, stomach cold emesis, and cough due to lung cold, and also has effects in relieving fish and crab toxin, relieving exterior syndrome, dispelling cold, warming spleen and stomach, relieving vomit, warming lung, relieving cough, and removing toxic substance; groundsel, bitter in taste, cold in nature; enters lung, liver and large intestine channels, and has the effects of clearing away heat and toxic materials, removing liver heat and improving eyesight. It is mainly used for treating carbuncle, swelling, sore, conjunctival congestion, swelling and pain, diarrhea and dysentery, epidemic qi, jaundice, malaria, and toxic by way of noxious insect and snake, and it is pounded and applied to the affected area such as sore, insect, snake and dog after being decocted and taken; medlar, sweet in taste and mild in nature; enters liver, kidney and lung channels, and has the effects of nourishing liver and kidney, replenishing vital essence and improving eyesight. Mainly treats the syndrome of liver-kidney yin deficiency and premature senility, tonifies essence and qi, strengthens vagina, strengthens tendons, resists aging, removes wind, tonifies muscles and bones, can benefit people and removes consumptive disease; american ginseng, sweet in taste, slightly bitter and cool in nature; enters lung, heart, kidney and spleen channels, and has the effects of tonifying qi and yin, clearing heat and promoting fluid production. Mainly treats the syndrome of qi and yin deficiency, the syndrome of lung qi deficiency and lung yin deficiency, thirst and diabetes caused by deficiency of body fluid due to deficiency of heat disease, and can obviously improve the survival rate of rats with hemorrhagic shock; has tranquilizing effect on brain, and has moderate exciting effect on life center; also has antihypoxic, anti-myocardial ischemia, anti-myocardial oxidation, myocardial contractility increasing, antiarrhythmic, antifatigue, anti-stress, anticonvulsant, blood glucose lowering, hemostatic and diuretic effects; lycopodium clavatum, bitter and slightly pungent in flavor, warm in nature; enters liver, spleen and kidney meridians, and has the effects of dispelling wind and removing dampness, and relaxing tendons and activating collaterals. Can be used for treating joint pain, difficulty in flexion and extension, and regulating nerve center.

The invention has the following beneficial effects: the traditional Chinese medicine compound preparation has obvious improvement effect on mental diseases such as migraine, depression and the like, has the characteristics of low toxicity and high efficiency, has the characteristics of improving symptom identification, small side effect, low afterburning rate and the like, has the characteristics of equivalent or superior effect to a positive control medicine, is prepared into a slow release preparation, and has long-acting effect and slow release effect.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The raw materials comprise the following components in parts by weight: 5 parts of garden balsam stem, 10 parts of weeping forsythia, 5 parts of Chinese starjasmine stem, 2 parts of cortex albiziae, 10 parts of blood Chinese angelica, 2 parts of platycladi seed, 15 parts of clematis root, 2 parts of ephedra, 1 part of prepared monkshood, 1 part of folium artemisiae argyi, 2 parts of ginger, 2 parts of murraya paniculata, 1 part of medlar, 1 part of American ginseng and 1 part of lycopodium clavatum.

The method comprises the following steps:

s1, weighing all the traditional Chinese medicine materials in proportion, respectively cleaning, drying, grinding into powder, and sieving to obtain traditional Chinese medicine powder, wherein the particle size of a sieved sieve mesh is 100 meshes;

s2, adding water into the Chinese medicine powder obtained in the step S1, wherein the solid-to-liquid ratio is 1:10g/mL, decocting for 3 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the obtained filtrate under reduced pressure to relative density of 1.2 to obtain water extract 100g, adding sodium alginate 20g, and mixing to obtain solution A;

s3, adding 5 wt% of acetic acid solution into the solid obtained in the step S2, wherein the solid-to-liquid ratio is 1:5g/mL, performing reflux extraction for 2 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.0 to obtain 50g of acid extract, adding 7.5g of chitosan, and uniformly mixing to obtain a solution B;

s4, preparing a 5 wt% calcium chloride solution to obtain a calcium chloride curing agent;

s5, adding the solution A obtained in the step S2 into the solution B obtained in the step S3, uniformly mixing, and adding 20mL of the calcium chloride curing agent obtained in the step S4 into the mixed solution to form gel, so as to obtain the traditional Chinese medicine composition hydrogel for treating mental diseases.

Example 2

The raw materials comprise the following components in parts by weight: 10 parts of garden balsam stem, 13 parts of weeping forsythia, 12 parts of Chinese starjasmine stem, 5 parts of cortex albiziae, 20 parts of blood Chinese angelica, 5 parts of platycladi seed, 25 parts of clematis root, 5 parts of ephedra, 4 parts of prepared common monkshood mother root, 2 parts of Chinese mugwort leaf, 4 parts of ginger, 5 parts of murraya paniculata, 3 parts of medlar, 2 parts of American ginseng and 4 parts of lycopodium clavatum.

The method comprises the following steps:

s1, weighing all the traditional Chinese medicine materials in proportion, respectively cleaning, drying, grinding into powder, and sieving to obtain traditional Chinese medicine powder, wherein the particle size of a sieved sieve mesh is 200 meshes;

s2, adding water into the Chinese medicine powder obtained in the step S1, wherein the solid-to-liquid ratio is 1:20g/mL, decocting for 5 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the obtained filtrate under reduced pressure to relative density of 1.5 to obtain water extract 100g, adding sodium alginate 35g, and mixing to obtain solution A;

s3, adding 15 wt% of acetic acid solution into the solid obtained in the step S2, wherein the solid-to-liquid ratio is 1:10g/mL, performing reflux extraction for 3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.3 to obtain 50g of acid extract, adding 10g of chitosan, and uniformly mixing to obtain a solution B;

s4, preparing a 10 wt% calcium chloride solution to obtain a calcium chloride curing agent;

Example 3

The raw materials comprise the following components in parts by weight: 6 parts of garden balsam stem, 11 parts of weeping forsythia, 6 parts of Chinese starjasmine stem, 3 parts of silktree albizzia bark, 12 parts of blood Chinese angelica, 3 parts of platycladi seed, 17 parts of clematis root, 3 parts of ephedra, 2 parts of prepared common monkshood mother root, 1.2 parts of Chinese mugwort leaf, 2.5 parts of ginger, 3 parts of murraya paniculata, 1.5 parts of medlar, 1.2 parts of American ginseng and 2 parts of lycopodium clavatum.

The method comprises the following steps:

s1, weighing all the traditional Chinese medicine materials in proportion, respectively cleaning, drying, grinding into powder, and sieving to obtain traditional Chinese medicine powder, wherein the particle size of a sieved sieve mesh is 150 meshes;

s2, adding water into the Chinese medicine powder obtained in the step S1, wherein the solid-to-liquid ratio is 1:20g/mL, decocting for 4 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the obtained filtrate under reduced pressure to relative density of 1.34 to obtain water extract 100g, adding sodium alginate 25g, and mixing to obtain solution A;

s3, adding 10 wt% of acetic acid solution into the solid obtained in the step S2, wherein the solid-to-liquid ratio is 1:10g/mL, performing reflux extraction for 3 times, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.12 to obtain 50g of acid extract, adding 17g of chitosan, and uniformly mixing to obtain a solution B;

s4, preparing a 7 wt% calcium chloride solution to obtain a calcium chloride curing agent;

Example 4

The raw materials comprise the following components in parts by weight: 9 parts of garden balsam stem, 12 parts of weeping forsythia, 10 parts of Chinese starjasmine stem, 4 parts of silktree albizzia bark, 18 parts of blood Chinese angelica, 4 parts of platycladi seed, 22 parts of clematis root, 4 parts of ephedra, 3 parts of prepared common monkshood mother root, 1.8 parts of Chinese mugwort leaf, 3.5 parts of ginger, 4 parts of murraya paniculata, 2.5 parts of medlar, 1.9 parts of American ginseng and 3 parts of lycopodium clavatum.

The method comprises the following steps:

s2, adding water into the Chinese medicine powder obtained in the step S1, wherein the solid-to-liquid ratio is 1:20g/mL, decocting for 4 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the obtained filtrate under reduced pressure to relative density of 1.34 to obtain water extract 100g, adding sodium alginate 30g, and mixing to obtain solution A;

Example 5

The raw materials comprise the following components in parts by weight: 7 parts of garden balsam stem, 12 parts of weeping forsythia, 8 parts of Chinese starjasmine stem, 3.2 parts of silktree albizzia bark, 14 parts of blood angelica, 3.5 parts of platycladi seed, 19 parts of clematis root, 3.4 parts of ephedra, 2.8 parts of prepared common monkshood mother root, 1.4 parts of argy wormwood leaf, 2.8 parts of ginger, 3.2 parts of murraya paniculata, 1.7 parts of medlar, 1.4 parts of American ginseng and 2.2 parts of lycopodium clavatum.

The method comprises the following steps:

Example 6

The raw materials comprise the following components in parts by weight: 8 parts of garden balsam stem, 11.2 parts of weeping forsythia, 9 parts of Chinese starjasmine stem, 3.7 parts of cortex albiziae, 16 parts of blood angelica, 3.8 parts of platycladi seed, 20 parts of clematis root, 3.8 parts of ephedra, 2.3 parts of prepared common monkshood mother root, 1.7 parts of argy wormwood leaf, 3.2 parts of ginger, 3.6 parts of murraya paniculata, 2.2 parts of medlar, 1.7 parts of American ginseng and 2.5 parts of lycopodium clavatum.

The method comprises the following steps:

Comparative example 1

Compared to example 6, only the water extract was used, and the other conditions were unchanged.

The method comprises the following steps:

s3, adding 17g of chitosan into 50g of 10 wt% acetic acid solution, and uniformly mixing to obtain a solution B;

Comparative example 2

Compared to example 6, only the acid extract was used, and the other conditions were unchanged.

The method comprises the following steps:

s2, adding 30g of sodium alginate into 100g of water, and uniformly mixing to obtain a solution A;

s3, adding 10 wt% of acetic acid solution into the traditional Chinese medicine powder obtained in the step S1, performing reflux extraction for 3 times at a solid-to-liquid ratio of 1:10g/mL, combining the extracting solutions, filtering, concentrating the filtrate under reduced pressure until the relative density is 1.12 to obtain 50g of acid extract, adding 17g of chitosan, and uniformly mixing to obtain a solution B;

Comparative example 3

The method comprises the following steps:

s2, adding water into the Chinese medicine powder obtained in the step S1, wherein the solid-to-liquid ratio is 1:15g/mL, decocting for 4 times to obtain water decoction, mixing the water decoction, filtering, and keeping the solid for use; concentrating the filtrate under reduced pressure to relative density of 1.34 to obtain water extract 100 g;

s3, adding 10 wt% of acetic acid solution into the solid obtained in the step S2, wherein the solid-to-liquid ratio is 1:10g/mL, performing reflux extraction for 3 times, combining the extracting solutions, filtering, and concentrating the filtrate under reduced pressure until the relative density is 1.12 to obtain 50g of acid extract;

s4, mixing the water extract obtained in the step S2 with the acid extract obtained in the step S3, and freeze-drying to obtain the traditional Chinese medicine composition for treating mental diseases.

Test example 1 preventive Effect on migraine in rat

1.1 laboratory animals, drugs, main reagents and instruments:

88 clean grade healthy male Wistar rats, body mass (170 + -10) g, provided by Yinshi laboratory animal technology, Inc., Catharan city, animal certification number: SCKK (Ji) 2018-. The adaptive feeding is carried out for 1 week before the experiment, rats (88) with good state are selected to be taken into the experiment, the rats are raised in cages, the room temperature is controlled to be 15-25 ℃, the humidity is controlled to be 50-70%, and the rats are naturally illuminated. The rats are guaranteed to eat and drink water freely, and the sterile padding is replaced for 1 time every 3 days.

The hydrogels of the Chinese medicinal compositions for treating mental diseases prepared in examples 1 to 6 and comparative examples 1 to 3 were prepared to suspensions of desired concentrations using physiological saline at the time of experiments.

Nitroglycerin is produced by Beijing Yimin pharmaceutical industry Co Ltd, NO detection kit is purchased from Nanjing to build bioengineering institute, CGRP and ETELISA detection kit are purchased from Beijing Biyuntian biotechnology institute

1.2 preparation and administration of the migraine rat model

88 Wistar male rats were randomly divided into 11 groups of 8 rats each, and each group was a normal control group (physiological saline 0.1mL 10 g)^-1) Migraine model group (physiological saline 0.1mL 10 g)^-1) Examples 1 to 6 and comparative examples 1 to 3 groups (100 mg. kg)^-1) The rats in each group are continuously administrated by gastric gavage for 7 days, and after the last administration, the rats in the migraine model group and each administration group are injected with nitroglycerin 10 mg-kg subcutaneously^-1The model is made for 1 time, subcutaneous injection is mainly positioned in the frontal area, the temporal area or the right shoulder subcutaneous injection, and normal control rats are injected with normal saline. After 3min of modeling, the animals are successively suffered from dysphoria, red ears, frequent head scratching of forelimbs and the like. This phenomenon lasts for about 3 hours, followed by a state of rolling, decreased activity and calmness, indicating that the migraine animal model replicates successfully.

1.3 serum NO level detection in rats of various groups

4h after molding, the rats of each group were treated with urethane (100 mg. kg)^-1) The abdominal cavity is injected for anesthesia, 1mL of blood is taken from the abdominal aorta, serum is separated, and the NO level in the serum of each group of rats is determined by adopting a nitrate reductase method.

1.4 detection of CGRP and ET levels in plasma in groups of rats

3mL of the abdominal aorta of the rat was bled, and 30. mu.L of 10% disodium Ethylenediaminetetraacetate (EDTA) and 10. mu.L of aprotinin (100U. mL of the composition) were injected^-1Above) at 4 ℃ and 3000 r.min in a test tube^-1Plasma was separated by centrifugation for 10min and CGRP and ET levels were measured in plasma by ELISA.

1.5 detection of NO levels in brain tissue of rats in groups

The rat is bled, the brain tissue is taken out by cutting off the head on an ice bench, the brain stem, the thalamus and the cortex tissue are quickly separated, the blood stain is removed by flushing with ice-cold normal saline, the brain tissue homogenate with the concentration of 10 percent is prepared by weighing the mass, and the NO level in the brain tissue of the rat is detected.

1.6 statistical analysis was performed using SPSS20.0 statistical software. NO, CGRP and ET levels in plasma, NO levels in brain tissue expressed as x ± s; the mean number between groups is compared by adopting one-factor variance analysis, and the pairwise comparison between groups is carried out by adopting t test. The difference is statistically significant when P is less than 0.05.

2. Results

2.1 levels of NO in serum and brain tissue of rats in groups

Nitroglycerin is the NO donor. After 4 hours of injecting nitroglycerin into rats in the migraine model group, the NO level in serum and brain tissue is obviously higher than that in a normal control group. See table 1. The groups 1 to 6 of the invention can obviously reduce the NO level rise caused by nitroglycerin, and have significance.

TABLE 1 comparison of NO levels

Group of	Serum (μmol. L)^-1)	Brain tissue (μmol. L)^-1)
			Normal control group	2.4±1.0	125±22
Model set	23.1±5.2**	162±29*
			Example 1	13.2±2.5##	138±24#
Example 2	12.1±2.3*	134±21#*
			Example 3	10.2±2.0**	132±24#*
Example 4	8.5±1.5#*	130±21#
			Example 5	8.9±1.3##*	133±22**
Example 6	8.2±1.4##**	127±20##**
			Comparative example 1	17.2±4.2	152±25
Comparative example 2	16.9±4.5	149±26
			Comparative example 3	14.2±3.9*	140±21#

Note that: p <0.05, P <0.01 compared to normal group; compared to the model group, # P <0.05, # P < 0.01.

2.2 plasma CGRP and ET levels in groups of rats

Compared with a normal control group, the plasma levels of CGRP and ET in the rats of the migraine model group are obviously increased. See table 2. The groups 1-6 of the invention can obviously reduce the increase of CGRP and ET levels, and have significance.

TABLE 2

Group of	CGRP(ng·L^-1)	ET(ng·L^-1)
			Normal control group	112.4±8.0	122.3±11.2
Model set	133.1±8.9*	137.5±8.2*
			Example 1	120.2±5.3#	130.2±6.9#
Example 2	121.5±4.9*	127.4±7.8#**
			Example 3	119.4±5.9*#	125.7±6.2##*
Example 4	117.5±6.7#**	123.2±5.8##
			Example 5	114.9±2.4##*	123.0±7.8*
Example 6	113.2±3.2##**	122.9±4.1##**
			Comparative example 1	129.5±4.5	132.5±9.5
Comparative example 2	128.4±5.3	134.5±8.4
			Comparative example 3	125.8±4.1*	129.4±9.2#

Nitroglycerin is a donor of NO, is easy to enter the brain through a blood brain barrier, and is firstly converted into NO when entering the body. NO binds to receptor, promotes the synthesis of cyclic guanosine monophosphate (cGMP), reduces the activity of K + channel of cell membrane, and inhibits cell Ca²⁺Flow, relaxation of vascular smooth muscle, dilation of cerebral vessels, triggering vasodilatory headaches; on the other hand, NO can activate peripheral trigeminal nerve endings of cerebral vessels, mediate release of various vasoactive substances (such as CGRP and the like) and generate neurogenic inflammationAnd (4) symptoms. A series of clinical or animal experimental researches prove that: NO levels in blood and cerebrospinal fluid of migraine sufferers or animals are significantly elevated and can be reduced and migraine symptoms alleviated by effective drug treatment. In the research, after nitroglycerin is injected, the NO level in the brain tissue and blood of a rat is obviously increased, and the success of migraine model modeling is prompted. The Chinese medicinal compositions for treating mental diseases prepared in examples 1-6 can obviously reduce the NO level in blood and brain tissues, which shows that the medicament has a preventive effect on migraine.

CGRP is a polypeptide containing 37 amino acid residues, is the strongest vasodilator active substance in vivo, plays an important role in the migraine attack process, and is considered as a new target point for treating migraine. CGRP is released in large amounts during migraine attacks and has the following effects on the body: promoting sensory nerve conduction, expanding blood vessels and activating CGRP (receptor of tumor necrosis factor); ② mediating degranulation of mast cells and releasing various active substances; the second-level neurons participating in nociceptors in the trigeminal nerve spinal nucleus influence the transmission of nociceptive signals; and fourthly, the glial cells in the trigeminal ganglia contain a large number of CGRP receptors, the CGRP can be directly synthesized, the glial cells are stimulated to secrete inflammatory factors and NO, and the NO can further release the CGRP and excite adjacent neurons and glial cells. Clinically effective anti-migraine drugs, such as ergotamines, sumatriptan and the like, can obviously inhibit the increase of the CGRP level in the plasma of migraine patients or migraine model animals. In the study, after nitroglycerin is injected, the level of CGRP in the plasma of a rat is obviously increased, and the success of model building of the migraine model is prompted. The plasma levels of CGRP in rats treated with the Chinese medicinal compositions for treating mental disorders prepared in examples 1 to 6 were significantly reduced, and it is considered that they have an effect of preventing migraine.

ET is a vasoactive polypeptide with strong vasoconstrictive action and is a major factor in headache and cerebral ischemia. In migraine attacks, ET levels in plasma are elevated, multiple cellular signaling pathways are activated, and the release or degradation of active substances that innervate blood vessels is imbalanced, causing vasomotor dysfunction, cerebral vasospasm, and resulting headache attacks. In the study, after nitroglycerin is injected, the CGRP level in the plasma of a rat is obviously increased, and the success of model building of the migraine model is prompted. The plasma ET levels in rats administered with the herbal compositions for the treatment of psychiatric disorders prepared in examples 1-6 were significantly reduced, suggesting that the drug's action in preventing migraine is related to the regulation of the in vivo vasoactive peptide balance.

Test example 2 antidepressant test

1.1 laboratory animals, drugs, main reagents and instruments:

88 clean grade healthy male ICR rats, 20 + -2 g in body mass, were purchased from the laboratory animal center of the institute of radiology, academy of medical sciences, China, and have a certification number of SCXK 2015-0005. The adaptive feeding is carried out for 1 week before the experiment, rats (88) with good state are selected to be included in the experiment, the rats are raised in cages, the room temperature is controlled to be 22 +/-2 ℃, the humidity is 60-70%, and the rats are naturally illuminated. The rats are guaranteed to eat and drink water freely, and the sterile padding is replaced for 1 time every 3 days.

Clomipramine hydrochloride tablets (Beijing Nowa pharmaceutical Co., Ltd., lot: X1012, 25 mg/tablet).

1.2 Experimental animal dosing regimens

88istar male rats were randomly divided into 11 groups of 8 rats, each group was a blank control group (physiological saline 0.1mL 10 g)^-1) Examples 1 to 6 and comparative examples 1 to 3 groups (100 mg. kg)^-1) Positive control group (Clomipramine hydrochloride 20 mg/kg)^-1) And performing intragastric administration for 1 time at 9: 00-10: 00 a.m. every day, and performing the test after continuous intragastric administration for 14 days.

1.3 mouse Tail suspension experiment

The part of the tail part of the mouse, which is 1-2 cm away from the tail part of the mouse, is attached to a horizontal wooden stick, so that the animal is in an inverted hanging state, the head part of the animal is about 20cm away from the table board, and the sight line of the animal is separated by boards at two sides of the hanging. Observe for 6min and record the immobility time of the mice as disappointment time within 4 min.

1.4 forced swimming test of mice

The dosing schedule was as above, with the experiment performed 1h after the last dose. Before the experiment, the mouse is trained to swim for 6min, and after 24h, the mouse is placed in a glass jar with the water depth of 10cm (the height is 20cm, the diameter is 14cm) and the water temperature is 25 +/-2 ℃. Observe for 6min, record and compare groups after 4min mice immobility time. The criterion for the immobility of the animals was that the mice slightly curled the body, but kept in a vertical position with the nostrils exposed to the water.

1.5 statistical analysis was performed using SPSS20.0 statistical software. Data are expressed in x ± s; the mean number between groups is compared by adopting one-factor variance analysis, and the pairwise comparison between groups is carried out by adopting t test. The difference is statistically significant when P is less than 0.05.

2. Results

2.1 influence of immobility time of mouse tail suspension

The results of comparing the time of immobility of the tail suspension of each group of mice with that of the blank control group are shown in Table 3. The results show that the groups of examples 1 to 6 and the positive control group can obviously shorten the immobility time of the mice and show the antidepressant effect. The remaining groups showed no antidepressant effect.

2.2 Effect on immobility time of forced swimming mice

The results of comparing the swimming immobility time of each group of mice with the blank control group are shown in Table 3, and it can be seen from the table that the immobility time of forced swimming mice can be significantly shortened by the groups of examples 1-6 compared with the positive control group, and the groups have significant difference (P <0.05) after administration compared with the blank control group. The immobility time of the forced swimming mice can be shortened to different degrees by other groups, but the difference is not significant.

TABLE 3

Note that: p <0.05, P <0.01 compared to normal group.

Compared with the prior art, the traditional Chinese medicine compound preparation has obvious improvement effect on mental diseases such as migraine, depression and the like, has the characteristics of low toxicity and high efficiency, has the characteristics of improving symptoms, small side effect, low reburning rate and the like, has the characteristics of low toxicity and high efficiency, and has long-acting effect and slow-releasing effect.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. The traditional Chinese medicine composition for treating mental diseases is characterized by being prepared from the following raw materials in parts by weight: 5-10 parts of garden balsam stem, 10-13 parts of weeping forsythia, 5-12 parts of Chinese starjasmine stem, 2-5 parts of silktree albizzia bark, 10-20 parts of blood angelica, 2-5 parts of platycladi seed, 15-25 parts of clematis root, 2-5 parts of ephedra, 1-4 parts of prepared common monkshood mother root, 1-2 parts of argy wormwood leaf, 2-4 parts of ginger, 2-5 parts of groundsel, 1-3 parts of medlar, 1-2 parts of American ginseng and 1-4 parts of lycopodium clavatum.

2. The traditional Chinese medicine composition for treating mental diseases according to claim 1, which is prepared from the following raw materials in parts by weight: 6-9 parts of garden balsam stem, 11-12 parts of weeping forsythia, 6-10 parts of Chinese starjasmine stem, 3-4 parts of cortex albiziae, 12-18 parts of blood angelica, 3-4 parts of platycladi seed, 17-22 parts of clematis root, 3-4 parts of ephedra, 2-3 parts of prepared common monkshood mother root, 1.2-1.8 parts of argy wormwood leaf, 2.5-3.5 parts of ginger, 3-4 parts of groundsel, 1.5-2.5 parts of medlar, 1.2-1.9 parts of American ginseng and 2-3 parts of lycopodium clavatum.

3. The traditional Chinese medicine composition for treating mental diseases according to claim 2, which is prepared from the following raw materials in parts by weight: 7 parts of garden balsam stem, 12 parts of weeping forsythia, 8 parts of Chinese starjasmine stem, 3.2 parts of silktree albizzia bark, 14 parts of blood angelica, 3.5 parts of platycladi seed, 19 parts of clematis root, 3.4 parts of ephedra, 2.8 parts of prepared common monkshood mother root, 1.4 parts of argy wormwood leaf, 2.8 parts of ginger, 3.2 parts of groundsel, 1.7 parts of medlar, 1.4 parts of American ginseng and 2.2 parts of common clubmoss herb.

4. The traditional Chinese medicine composition for treating mental diseases according to claim 2, which is prepared from the following raw materials in parts by weight: 8 parts of garden balsam stem, 11.2 parts of weeping forsythia, 9 parts of Chinese starjasmine stem, 3.7 parts of silktree albizzia bark, 16 parts of blood Chinese angelica, 3.8 parts of platycladi seed, 20 parts of clematis root, 3.8 parts of ephedra, 2.3 parts of prepared common monkshood mother root, 1.7 parts of argy wormwood leaf, 3.2 parts of ginger, 3.6 parts of groundsel, 2.2 parts of medlar, 1.7 parts of American ginseng and 2.5 parts of lycopodium clavatum.

5. A method for preparing the Chinese medicinal composition for treating mental diseases according to any one of claims 1 to 4, which comprises the following steps:

6. The method as claimed in claim 5, wherein the sieved mesh size in step S1 is 100-200 mesh.

7. The method of claim 5, wherein the sodium alginate is added in an amount of 20-35 wt% of the aqueous extract in step S2.

8. The method according to claim 5, wherein the chitosan is added in an amount of 15-20 wt% to the acid extract in step S3.

9. The preparation method according to claim 5, wherein the solid-to-liquid ratio of the Chinese medicinal powder to water in step S2 is 1: (10-20) g/mL.

10. The production method according to claim 5, wherein the solid-to-liquid ratio of the solid to the acetic acid solution in step S3 is 1: (5-15) g/mL, and the mass percent concentration of the acetic acid solution is 5-15 wt%.