CN112715957A - Biological small molecular peptide composition for dispelling effects of alcohol and protecting liver and preparation method thereof - Google Patents
Biological small molecular peptide composition for dispelling effects of alcohol and protecting liver and preparation method thereof Download PDFInfo
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- CN112715957A CN112715957A CN202011407436.4A CN202011407436A CN112715957A CN 112715957 A CN112715957 A CN 112715957A CN 202011407436 A CN202011407436 A CN 202011407436A CN 112715957 A CN112715957 A CN 112715957A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention relates to the technical field of health-care food, in particular to a biological small molecular peptide composition for dispelling effects of alcohol and protecting liver and a preparation method thereof. The active ingredients of the composition comprise mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide, sesame peptide and marine peptide, the peptides are small molecular peptide substances obtained by biological compound enzyme enzymolysis protein and a directional enzyme cutting technology, the beneficial nutrient components of plants and marine organisms are reserved, the absorption speed of a human body is higher than that of single amino acid or protein, the human body can be promoted to metabolize ethanol, the damage of the ethanol to human tissues and cells is reduced, and the detoxifying capacity of the liver function of the human body to the ethanol and the acetaldehyde is improved. The preparation method of the composition adopts the modes of high-temperature decoction and pressurization low-temperature concentration to prepare the composition, and is convenient and quick.
Description
Technical Field
The invention relates to the technical field of health-care food, in particular to a biological small molecular peptide composition for dispelling effects of alcohol and protecting liver and a preparation method thereof.
Background
In order to solve the problem of protecting liver and alleviating hangover, a large amount of classical data are consulted and a safe and effective solution is summarized by combining modern biotechnology. According to the theory of liver and kidney homology, the theory of sobering up and protecting liver first and treating liver and kidney simultaneously, the theory of plain inquiry and five operation theory, namely the northern cold, cold and water generation, aquatic salt, salty and kidney generation, kidney and marrow generation and liver generation, discloses the close relation of mutual connection and mutual influence between the liver and the kidney, and embodies the mutual holistic concept of the traditional Chinese medicine. The philosophy of liver and kidney homology is originated from the book of changes, the medical foundation is originated from the book of internal Jing, the clinical practice is rich in the Jinyuan of Han Tang Dynasty, the theoretical system is formed in the Ming dynasty, the experimental research is developed in the modern, the theory of Yin-Yang correspondence is reported in the book of Shen Sheng Gu and medulla Sheng Gao, the book of Shen, the records of liver storing blood, blood storing soul, liver qi deficiency fear, fear of unrelied, sperm injury and sperm injury are caused by bone soreness and fainting "
At present, the types of the dealcoholic products are various, most of the dealcoholic products are beverage type or tablet type products with the function of relieving alcoholism, only one product aims at relieving alcoholism, most of the dealcoholic products adopt cool components such as Japanese raisin Tree seed, cassia seed, mint, chrysanthemum, lophatherum gracile, kudzu root and the like which are added with Chinese herbal medicine components, and the cool components are similar to herbal tea, so people with cold constitution are easy to cause, kidney burden is generated, the dealcoholic products are not suitable for long-term drinking, and the dealcoholic products are.
CN 109432386A discloses a corn peptide composition for relieving alcoholism and protecting liver and a preparation method thereof, the method comprises the following steps of mixing kudzu root, Japanese raisin Tree seed, medlar and turmeric according to a ratio, boiling twice, filtering by a plate frame, then carrying out low-temperature reduced pressure concentration and spray drying on the filtrate, and finally mixing the product obtained by spray drying with corn peptide.
Disclosure of Invention
In order to overcome the defects of the prior art, one of the purposes of the invention is to provide a biological small molecular peptide composition for relieving alcoholism and protecting liver, which consists of plant peptide and marine peptide and has the functions of protecting liver, nourishing stomach and kidney; the invention also aims to provide a preparation method of the biological small molecular peptide composition for relieving alcoholism and protecting liver, which can effectively preserve the nutrient components of the plant peptide and the marine peptide and is convenient and easy to obtain.
One of the purposes of the invention is realized by adopting the following technical scheme:
a biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following active ingredients: marine and plant peptides; wherein the marine peptide is one or a combination of more than two of hippocampal peptide, oyster peptide or sea cucumber peptide. The hippocampal peptide, oyster peptide and sea cucumber peptide have the functions of regulating blood fat, inhibiting platelet aggregation, improving hyperglycemia symptoms and enhancing human immunity. Specifically, the marine peptide is prepared into small molecular oligopeptide by enzymolysis. The nutritional ingredients such as original vitamins, trace elements, taurine and the like of the oysters, the sea horses and the sea cucumbers are completely reserved, the ingestion speed of a human body is faster than that of single amino acid or protein, the absorption by the human body is easier, and the biological activity is more important in the aspect of human metabolism.
The hippocampal peptide can effectively block the channel of human body for absorbing calcium ions, so that the hippocampal peptide is frequently used for blocking calcium ion inflow and protecting the function of neurons in clinic. The hippocampal peptide has obvious antioxidant performance, can reduce the content of peroxidized lipid in human body and reduce the activity of monoamine oxidase, thereby having the function of resisting aging.
The sea cucumber peptide powder is rich in sea cucumber mucopolysaccharide (acidic mucopolysaccharide), sea cucumber saponin (holothurin), sea cucumber collagen, 18 amino acids, taurine and the like, and also contains a large amount of trace and major beneficial elements required by human bodies, such as selenium, zinc, calcium, manganese, phosphorus, iron and the like.
The oyster peptide contains DHA, EPA, taurine, various vitamins and trace elements such as calcium, phosphorus, iron, zinc and the like. The oyster peptide has the effects of protecting liver cells and inhibiting liver cell apoptosis through antioxidation. Taurine can resist lipid peroxidation, protect liver cells, inhibit liver cell apoptosis, and improve liver detoxification capability. The oyster peptide can increase the relative weight of immune organs and the content of serum hemolysin, can obviously increase antibody-producing cells and delayed allergy, and has the function of enhancing immunity. Oyster polysaccharide contained in oyster oligopeptide can enhance organism immunity and inhibit influenza virus proliferation.
Further, the plant peptide is one or a combination of more than two of mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide or sesame peptide.
The soybean oligopeptide can regulate blood sugar, reduce blood fat and cholesterol, and improve liver function.
The corn peptide can inhibit the absorption of stomach to alcohol, increase the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in vivo, and promote the metabolism and discharge of alcohol in vivo, and has high content of branched chain amino acids (leucine, isoleucine and valine) in the amino acid composition of corn peptide.
The wheat oligopeptide has effect of inhibiting cholesterol increase. The wheat oligopeptide can promote insulin secretion, and the functional substance is oligomethionine, and can be used for regulating blood sugar of human and improving diabetes symptoms. It also can inhibit the action of angiotensin enzyme, thus lowering blood pressure. One of the characteristics of the wheat oligopeptide is that the wheat oligopeptide contains homoglutamine, can effectively regulate nerves and can be used as a special nutrient substance in the case of intestinal dysfunction.
The mung bean peptide contains rich protein, can protect gastrointestinal mucosa by oral administration, and can be combined with organophosphorus pesticide, mercury, arsenic, lead and other toxic elements to form precipitate to reduce or lose toxicity, and is not easy to be absorbed by intestinal tract. Has obvious effect of reducing cholesterol in blood. The mung bean peptide is rich in various amino acids, wherein the content of cysteine is 2-3 times higher than that of mung bean protein. Animal experiments show that the mung bean peptide has obvious effect of reducing cholesterol in blood.
The ginseng peptide contains natural mineral chromium, can directly enter pancreas to repair damaged cells, activates the function of secreting pancreatic islets and is beneficial to normal glycometabolism; can also inhibit hepatic glycogen decomposition in liver, increase hepatic glycogen synthesis, achieve stable blood sugar lowering effect, ensure cell energy metabolism, and eliminate more than three and less than one. Leucine and isoleucine both have the effect of stabilizing blood glucose. Alanine and leucine in ginseng peptide have good effects of relieving anesthesia and preventing drunkenness, and glutamic acid and proline also have influence on alcohol metabolism. It can slow down the absorption of alcohol, promote alcohol metabolism, reduce toxicity, reduce acute alcoholism caused by heavy drinking, improve liver function, and protect liver.
The sesame peptide can be completely dissolved in water and is easy to be absorbed by intestinal tract. The sesame peptide takes sesame as a raw material, utilizes a bioengineering directional enzyme digestion technology to degrade macromolecular protein into micromolecular peptide with good solubility, emulsibility and bioactivity, improves the functional characteristics of the protein, and is more widely applied to common food and functional food. Can be quickly absorbed in vivo, and has high utilization rate. The sesame peptide has the function of reducing blood sugar: can reduce blood sugar and increase glycogen content in liver and muscle.
Still further, the beverage also comprises a taste modifier, and the taste modifier is citric acid and/or stevioside.
Further, the tea also comprises an antioxidant, wherein the antioxidant is green tea powder.
Furthermore, the peptide segment with the molecular weight of 100-1000 Da in the marine peptide accounts for more than 90% of the total amount, so that the nutritional ingredients in the marine peptide are easier to absorb, and the liver-protecting and hangover-alleviating effects are obvious.
Further, the plant peptide is a combination of six of mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide and sesame peptide; the biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following raw materials in parts by mass:
1-10 parts of oyster peptide, 2-8 parts of sea horse peptide, 2-8 parts of sea cucumber peptide, 2-16 parts of mung bean peptide powder, 3-9 parts of wheat oligopeptide powder, 2-10 parts of soybean oligopeptide powder, 4-10 parts of corn peptide, 2-9 parts of ginseng peptide and 5-15 parts of sesame peptide. The green tea beverage also comprises 0.5-3 parts of citric acid, 1-3 parts of stevioside and 2-6 parts of green tea powder. The mung bean peptide, the wheat oligopeptide, the soybean oligopeptide, the corn peptide and the ginseng peptide are matched with the oyster peptide, so that the effects of enhancing and reducing blood pressure, alleviating hangover, inhibiting cholesterol increase and resisting oxidation can be achieved, the growth of tissues and intelligence is promoted, the absorption of cells and the utilization of glucose are promoted, and the activity of an organism is promoted.
The second purpose of the invention is realized by adopting the following technical scheme:
the preparation method of the biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following steps:
and (3) putting the plant peptide, the marine peptide and purified water with the solid mass being 10-20 times of that of the plant peptide and the marine peptide into a high-pressure container, boiling for 1-3 h, and cooling to obtain the biological small molecular peptide composition for relieving alcoholism and protecting liver.
Further, the plant peptide, the marine peptide and purified water with the solid mass being 10-20 times that of the plant peptide and the marine peptide are placed into a high-pressure container to be boiled for 1-3 hours, and after the temperature is reduced to 40-60 ℃, green tea powder, citric acid and stevioside are added to obtain the alcohol-dispelling and liver-protecting biological small molecular peptide composition.
And further, after the temperature is reduced to 40-60 ℃, the pressure is set to be 0.02-0.08 MPa.
Compared with the prior art, the invention has the beneficial effects that:
(1) the plant peptide and the marine peptide selected by the invention belong to small molecular peptides, beneficial nutritional ingredients of plants and marine organisms are reserved, the absorption speed of a human body is faster than that of single amino acid or protein, and the plant peptide and the marine peptide are synergistic, so that the alcohol metabolism of the human body can be promoted, the damage of alcohol to human tissues and cells can be reduced, the detoxifying capability of the liver function of the human body on alcohol and acetaldehyde can be improved, and the effects of dispelling the effects of alcohol and protecting the liver can be achieved.
(2) The preparation method of the biological small molecular peptide composition for alleviating hangover and protecting liver adopts the biological small molecular peptide sheared by the special biological enzyme enzymolysis technology, so that the biological activity is stable. Even if the raw materials are decocted at high temperature and cooled to 40-60 ℃, the protein of the plant peptide and the marine peptide can still keep stable performance, the original nutritional ingredients are effectively preserved, the raw materials are easy to obtain, and the preparation method is simple and convenient.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
The peptide raw materials used in examples 1 to 5 and comparative examples 1 to 8 are all small molecule peptide substances obtained by enzymatic hydrolysis of protein by using biological complex enzyme and by using a directional enzyme cutting technology.
Example 1
The biological small molecular peptide composition for relieving alcoholism and protecting liver is prepared from the following components in parts by weight:
the feed additive comprises 16 parts of mung bean peptide, 9 parts of wheat oligopeptide, 10 parts of soybean oligopeptide, 10 parts of corn peptide, 9 parts of ginseng peptide, 15 parts of sesame peptide, 9 parts of oyster peptide, 8 parts of sea cucumber peptide, 8 parts of sea horse peptide, 3 parts of citric acid, 3 parts of stevioside and 6 parts of green tea powder.
The preparation method of the biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following steps:
adding mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide, sesame peptide, oyster peptide, sea cucumber peptide and sea horse peptide into a high-pressure container, adding 1060 parts of purified water, stirring, mixing, boiling for 2 hours, cooling to 40 ℃, pressurizing to 0.02MPa, adding citric acid, stevioside and green tea powder, and stirring to obtain a concentrated solution;
pumping the concentrated solution into a canning device, bottling according to 100ml per bottle, putting into a high-temperature furnace, pressurizing, adjusting temperature to 200 ℃, sterilizing and molding to obtain the biological small molecular peptide composition for relieving alcoholism and protecting liver.
Wherein, the oyster peptide is prepared by applying peptide molecular biotechnology to oyster processing and carrying out enzymolysis. The micromolecule oligopeptide formed by the preparation method completely reserves the original nutrients such as vitamins, trace elements, taurine and the like of the oysters, so that the oysters rich in nucleic acid can be absorbed by the human body more quickly than single amino acid or protein after being ingested by the human body, are absorbed by the human body more easily, have more important biological activity in the aspect of human metabolism, and can effectively improve the male serum testosterone level. Has higher biological value and more important physiological function than the common oyster products. The composition has liver and kidney nourishing effect when being matched with five kinds of plant peptides.
Example 2
The biological small molecular peptide composition for relieving alcoholism and protecting liver is prepared from the following components in parts by weight:
2 parts of mung bean peptide, 3 parts of wheat oligopeptide, 2 parts of soybean oligopeptide, 4 parts of corn peptide, 2 parts of ginseng peptide, 5 parts of sesame peptide, 1 part of oyster peptide, 2 parts of sea cucumber peptide, 2 parts of sea horse peptide, 0.5 part of citric acid, 1 part of stevioside and 2 parts of green tea powder.
The preparation method of the biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following steps:
adding mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide and oyster peptide into a high-pressure container, adding 460 parts of purified water, stirring, mixing, boiling for 3 hours, cooling to 60 ℃, pressurizing to 0.08MPa, then adding citric acid, stevioside and green tea powder, and stirring to obtain a concentrated solution;
pumping the concentrated solution into a canning device, bottling according to 100ml per bottle, putting into a high-temperature furnace, pressurizing, adjusting temperature to 200 ℃, sterilizing and molding to obtain the biological small molecular peptide composition for relieving alcoholism and protecting liver.
Example 3
The components and preparation methods of example 3 and example 1 are essentially the same, except that: only oyster peptide is added, and sea cucumber peptide and sea horse peptide are not added.
Example 4
The components and preparation of example 4 and example 1 are essentially the same, except that: only sea horse peptide is added, and sea cucumber peptide and oyster peptide are not added.
Example 5
The components and preparation methods of example 5 and example 1 are essentially the same, except that: only sea cucumber peptide is added, and sea horse peptide and oyster peptide are not added.
Comparative example 1
The components and preparation methods of comparative example 1 and example 1 are substantially the same, except that: oyster peptide, sea cucumber peptide and hippocampal peptide are not added.
Comparative example 2
The components and preparation methods of comparative example 2 and example 1 are substantially the same, except that: comparative example 2 no mung bean peptide was added.
Comparative example 3
The components and preparation methods of comparative example 3 and example 1 are substantially the same, except that: comparative example 3 no wheat oligopeptide was added.
Comparative example 4
The components and preparation methods of comparative example 4 and example 1 are substantially the same, except that: comparative example 4 no soy oligopeptide was added.
Comparative example 5
The components and preparation methods of comparative example 5 and example 1 are substantially the same, except that: comparative example 5 no corn peptide was added.
Comparative example 6
The components and preparation methods of comparative example 6 and example 1 are substantially the same, except that: comparative example 6 no ginseng peptide was added.
Comparative example 7
The components and preparation methods of comparative example 7 and example 1 are substantially the same, except that: comparative example 7 no sesame peptide was added.
Comparative example 8
The components and preparation methods of comparative example 8 and example 1 are substantially the same, except that: comparative example 8 no mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide and sesame peptide were added.
Functional test
1. Materials and apparatus
1.1 animals
SPF-grade KM mice, which are half male and female, have the body mass of 25-45g, are provided by experimental animal center of Guangxi medical university, and the production license number of the experimental animal is as follows: SCXK Ossa 2014-.
1.2 instruments and devices
Low temperature high speed centrifuge (KL 05R): kaida scientific instruments, Hunan; high speed centrifuge (KH 20R): kaida scientific instruments, Hunan; ultraviolet visible spectrophotometer (EU-2200): shanghai Onla instruments, Inc.; constant temperature water bath (HHSYZL-Ni): beijing Changfeng instruments Inc.; adjustable liquid transfer device: hangzhou De is Instrument science and technology, Inc.
2. Detailed experiments
2.1 anti-intoxication experiment
Taking 140 mice (70 male and female respectively, the error of the body mass of each group is not more than 10g), carrying out adaptive feeding for 3d, and then randomly dividing the mice into 14 groups, wherein each group comprises 10 mice. After fasting for 12 hours without water inhibition, 13 groups were given the compositions of examples 1 to 5 and comparative examples 1 to 8, respectively, and the 14 th group (normal group) was filled with physiological saline (0.15mL/10 g). After 30min of administration, 38% vol Guilin Sanhua wine (0.15mL/10g) was administered to each group of mice by intragastric administration, the product of Guilin Sanhua GmbH company Limited, and the mice were placed in a squirrel cage with their backs facing downward and light weight. If the mouse keeps the posture of keeping the back down for more than 30s, the righting reflex disappears, namely drunk, otherwise drunk. The tolerance time is the time from filling wine to disappearance of righting reflection, and the drunk time is the time from disappearance of righting reflection to restoration. The data are shown in Table 1.
2.2 protective action against Alcoholic liver injury
2.2.1 measurement of AST and ALT in serum
Taking 150 mice (75 males and females respectively, the error of the physical quality of each group is not more than 10g), carrying out adaptive feeding for 3d, and then randomly dividing the mice into 15 groups, wherein each group comprises 10 mice. After fasting for 12 hours without water inhibition, 13 groups were given the compositions of examples 1 to 5 and comparative examples 1 to 8, respectively, and the 14 th group (normal group) was filled with physiological saline (0.15mL/10 g). The normal group was gavaged with 0.3mL of physiological saline daily. In group 15 (model group), 0.3mL of 38 ° three-flower wine was intragastrically administered daily to prepare an alcoholic liver injury model. And (3) performing intragastric administration on 0.3mL of corresponding anti-alcoholism and liver-protecting composition every day in 1-11 groups (examples 1-5 and comparative examples 1-8), and performing intragastric administration on 0.3mL of 38-degree three-flower wine after 30 min. Each group of mice was gavaged continuously for 5 weeks. After 30min after the last day of gavage, blood is taken from eyeballs of the mice, serum is separated, and the vitality of ALT and AST in the serum of each group of mice is measured by using a spectrophotometer according to the instructions of an ALT (alanine aminotransferase) measuring kit and an AST (aspartate aminotransferase) measuring kit. The data are shown in Table 2.
2.2.2 determination of Oxidation/Oxidation related indices in liver
In experiment 2.2.1, after the blood of eyeballs is collected from each group of mice, the mice are dissected, the livers are taken out, the mass of the livers is accurately weighed, a proper amount of liver tissues is taken, 10% liver homogenate is prepared by taking 0.9% physiological saline as a homogenate medium, and supernatant is taken after centrifugation, and GSH-Px is detected for later use. Liver index (liver mass index) was calculated as liver mass/body weight × 100%. The activity of mouse liver homogenate GSH-Px was determined according to the glutathione peroxidase (GSH-Px) kit instructions. The data are shown in Table 3.
TABLE 1 statistics of intoxication time, tolerance time and intoxication rate for each group
TABLE 2 statistics of ALT and AST serum viability in each group of mice
TABLE 3 liver index and liver tissue GSH-Px viability of the groups of mice
| Group of | Liver index/% | SGH-Px/U·mg-1Protein |
| Example 1 | 4.72±2.02 | 203.11±2.34 |
| Example 2 | 4.88±1.90 | 196.87±2.25 |
| Example 3 | 4.81±2.43 | 198.36±2.42 |
| Example 4 | 4.79±1.58 | 201.26±1.77 |
| Example 5 | 4.86±2.69 | 200.97±2.29 |
| Comparative example 1 | 5.81±2.44 | 178.32±1.37 |
| Comparative example 2 | 5.26±1.63 | 190.44±1.87 |
| Comparative example 3 | 5.46±1.56 | 188.37±1.89 |
| Comparative example 4 | 5.44±1.47 | 188.23±2.15 |
| Comparative example 5 | 4.95±1.71 | 191.60±1.64 |
| Comparative example 6 | 5.02±1.84 | 194.18±5.88 |
| Comparative example 7 | 5.11±1.84 | 193.37±3.28 |
| Comparative example 8 | 5.67±2.99 | 182.83±6.16 |
| Normal group | 4.56±2.62 | 211.39±5.19 |
| Model set | 6.54±1.28 | 173.12±4.36 |
According to the data in table 1, the drunk tolerance time of the white mice is obviously increased compared with the normal mice, and the difference is significant (P is less than 0.01). The tolerance time of the mice in the embodiments 1 to 5 is longer than that of the normal group and the comparative examples 1 to 8, wherein the tolerance time and the drunk time of the embodiment 1 are longer than those of the embodiments 3 to 5, and the fact that the six plant peptide components selected by the invention are matched with the three marine peptides can improve the effect of dispelling the effects of alcohol, effectively prolong the drunk tolerance time of the mice, shorten the drunk time of the mice and increase the tolerance degree of the mice to alcohol is shown. But even if the six plant peptides are matched with one of oyster peptide, sea cucumber peptide or hippocampal peptide, the mouse anti-alcohol effect can be well achieved.
According to the data in table 2, the serum ALT and AST of the model group mice are obviously increased compared with the normal group, and have significant difference (P is less than 0.01), which indicates that alcohol damages the liver of the model group mice. The products of examples 1-5 and comparative examples 1-8 can effectively reduce ALT and AST, but obviously, the ALT and AST reduction amplitude of examples 1-5 is larger than that of comparative examples 1-8, mainly because the small molecular peptide composition for relieving alcoholism and protecting liver disclosed by the invention has multiple antioxidant components, has good antioxidant capacity, can effectively remove redundant free radicals and active oxygen in vivo, can also reduce the content of malondialdehyde in blood plasma, and inhibits the improvement of the enzymatic activities of ALT and AST in blood serum, so that hepatocytes are protected and the metabolism of ethanol is accelerated. The peptide raw materials in the embodiment are small molecular peptides sheared by a biological enzymolysis technology, and the molecular structures of the small molecular peptides keep activity and are easy to be absorbed by human bodies.
According to the data in table 3, the liver index of the white mouse in the model group subjected to white spirit gastric lavage in the experiment is increased compared with that of the white mouse in the normal group, and the GSH-Px activity in liver tissues is obviously reduced, so that the damage condition of the liver of the white mouse caused by long-term drinking is fully reflected. Each index of the mice in the examples 1-5 is better than that of the model group, which shows that the examples 1-5 can play a role in dispelling the effects of alcohol and protecting the liver. Each index of the mouse in comparative examples 1-8 is superior to that of the model group, but is lower than that in examples 1-5, which shows that the anti-intoxication and anti-alcoholism effects can be achieved by selecting and matching mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide and marine peptide (one or three of oyster peptide, sea cucumber peptide and sea horse peptide), and the liver-protecting and anti-alcoholism effects can be maximized by selecting and matching mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide, marine peptide, oyster peptide, sea cucumber peptide and sea horse peptide.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (10)
1. The biological small molecular peptide composition for relieving alcoholism and protecting liver is characterized by comprising the following active ingredients: marine and plant peptides; wherein the marine peptide is one or a combination of more than two of hippocampal peptide, oyster peptide or sea cucumber peptide.
2. The alcohol-relieving and liver-protecting biological small molecular peptide composition according to claim 1, wherein the plant peptide is one or a combination of more than two of mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide or sesame peptide.
3. The alcohol-relieving and liver-protecting biomolecular peptide composition according to claim 1, further comprising a taste modifier, wherein the taste modifier is citric acid and/or stevioside.
4. The alcohol relieving and liver protecting biomolecular peptide composition according to claim 1, further comprising an antioxidant, wherein the antioxidant is green tea powder.
5. The biological small molecular peptide composition for relieving alcoholism and protecting liver as claimed in claim 1, wherein the peptide segment with molecular weight of 100-1000 Da in the marine peptide accounts for more than 90% of the total amount.
6. The anti-alcohol and liver-protecting biomolecular peptide composition according to claim 1, wherein the plant peptide is a combination of six of mung bean peptide, wheat oligopeptide, soybean oligopeptide, corn peptide, ginseng peptide and sesame peptide; the biological small molecular peptide composition for relieving alcoholism and protecting liver comprises the following raw materials in parts by mass:
1-10 parts of oyster peptide, 2-8 parts of sea horse peptide, 2-8 parts of sea cucumber peptide, 2-16 parts of mung bean peptide powder, 3-9 parts of wheat oligopeptide powder, 2-10 parts of soybean oligopeptide powder, 4-10 parts of corn peptide, 2-9 parts of ginseng peptide and 5-15 parts of sesame peptide.
7. The alcohol relieving and liver protecting biological small molecular peptide composition according to claim 6, further comprising 0.5-3 parts of citric acid, 1-3 parts of stevioside and 2-6 parts of green tea powder.
8. The preparation method of the alcohol-relieving and liver-protecting biological small molecular peptide composition as claimed in any one of claims 1 to 7, characterized by comprising the following steps:
and (3) putting the plant peptide, the marine peptide and purified water with the solid mass being 10-20 times of that of the plant peptide and the marine peptide into a high-pressure container, boiling for 1-3 h, and cooling to obtain the biological small molecular peptide composition for relieving alcoholism and protecting liver.
9. The preparation method of the biological small molecular peptide composition for relieving alcoholism and protecting liver as claimed in claim 7, is characterized by comprising the following steps:
placing the plant peptide, the marine peptide and purified water with the solid mass being 10-20 times of that of the plant peptide and the marine peptide into a high-pressure container, boiling for 1-3 h, cooling to 40-60 ℃, and then adding green tea powder, citric acid and stevioside to obtain the alcohol-relieving and liver-protecting biological small molecular peptide composition.
10. The method for preparing the biological small molecular peptide composition for alleviating hangover and protecting liver as claimed in claim 9, wherein the pressure is set to 0.02 to 0.08MPa after the temperature is reduced to 40 to 60 ℃.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114568706A (en) * | 2022-02-16 | 2022-06-03 | 唐建 | Sobering-up compound peptide and preparation method thereof |
| CN114631565A (en) * | 2022-03-28 | 2022-06-17 | 唐建 | Beverage before drinking and preparation method thereof |
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| CN116473239A (en) * | 2023-04-17 | 2023-07-25 | 江苏恰瑞生物科技有限公司 | Protein peptide composition for relieving acute alcoholic liver injury and preparation method thereof |
| CN116473239B (en) * | 2023-04-17 | 2023-11-21 | 江苏恰瑞生物科技有限公司 | Protein peptide composition for relieving acute alcoholic liver injury and preparation method thereof |
| CN116790699A (en) * | 2023-06-21 | 2023-09-22 | 江苏恰瑞生物科技有限公司 | Preparation method of protein hydrolysate for treating acute alcoholic liver injury cell repair |
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