CN112715788A - Feed for improving survival rate of sturgeon juvenile fish and preparation method thereof - Google Patents
Feed for improving survival rate of sturgeon juvenile fish and preparation method thereof Download PDFInfo
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- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
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- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention provides a feed for improving the survival rate of sturgeon parr and a preparation method thereof, wherein the feed is prepared from the following raw materials in parts by weight: 10-20 parts of white fish meal, 30-50 parts of full-fat expanded soybean meal, 0.1-5 parts of yeast, 10-20 parts of liver meal, 10-20 parts of scallop powder, 10-20 parts of cuttlefish viscera powder, 1-10 parts of fish oil, 0.1-1 part of compound vitamin and 1-10 parts of plant extract composition. The feed for improving the survival rate of the juvenile sturgeon is prepared from the plant composition, has an antibacterial effect, and is not easy to decompose in water by coating the internal material with chitosan, so that the waste of the feed is prevented.
Description
Technical Field
The invention belongs to the field of aquatic products, and particularly relates to a feed for improving the survival rate of young sturgeons and a preparation method thereof.
Background
Sturgeons belong to the class Osteichthyes, subclasses Bangnariae, general order of hard scale and order of sturgeons, are mainly distributed in the river basin of Amore river (black Longjiang, China), have become popular breeding varieties in China due to the advantages of high growth speed, strong disease resistance, high economic value and the like, account for 50% of the total output of the sturgeons in China, and have a tendency of increasing. However, as the breeding technology is extensive and the breeding density is too high, the disease outbreak frequency is increased, and great obstruction is caused to the health sustainable development of the sturgeon breeding industry. The sturgeon has strict and confidential process and technology, the content of learning and reference is very little, and some domestic experts publish articles, so that the sturgeon does not eat compound feed. And the sturgeon fry is not stably cultivated at the time, and the first times of sturgeon fry fail to finish.
Disclosure of Invention
In view of the above, the invention aims to overcome the defects in the prior art and provides a feed for improving the survival rate of sturgeon parrs and a preparation method thereof.
In order to achieve the purpose, the technical scheme of the invention is realized as follows:
the feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: 10-20 parts of white fish meal, 30-50 parts of full-fat expanded soybean meal, 0.1-5 parts of yeast, 10-20 parts of liver meal, 10-20 parts of scallop powder, 10-20 parts of cuttlefish viscera powder, 1-10 parts of fish oil, 0.1-1 part of compound vitamin and 1-10 parts of plant extract composition.
Preferably, the feed comprises the following raw materials in parts by weight: 15-20 parts of white fish meal, 40-50 parts of full-fat expanded soybean meal, 0.1-5 parts of yeast, 12-20 parts of liver meal, 12-20 parts of scallop powder, 12-20 parts of cuttlefish viscera powder, 1-10 parts of fish oil, 0.1-1 part of compound vitamin and 1-5 parts of plant extract composition.
Further, the plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 10-100.
Preferably, the plant extract composition contains the pink bamboo extract and the white muscardine silkworm extract in a mass ratio of 1: 25-65.
Further, the extract of the dianthus chinensis is prepared by a method comprising the following steps: drying and grinding the dianthus chinensis into powder, then putting the powder into 20-30 times of 50-80% ethanol, leaching for 1-3 hours, repeatedly leaching for 3-5 times, then filtering, combining filtrates, and concentrating to obtain the dianthus chinensis extract.
Further, the bombyx batryticatus extract is prepared by a method comprising the following steps: grinding the white muscardine silkworm into powder, putting the powder into 10-20 times of water, heating for 30-60 minutes at 60-90 ℃, then filtering to obtain supernatant, concentrating the supernatant, adding 10-20 times of absolute ethyl alcohol, standing for 6-24 hours at 2-10 ℃, centrifuging, and drying precipitate to obtain the white muscardine silkworm extract.
The preparation method of the feed for improving the survival rate of the juvenile sturgeon comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) uniformly mixing the plant extract composition and the full-fat puffed soybean powder to obtain a premix B;
(3) adding the premix A into a main feeding port of a double-screw extruder, adding the premix B into a side feeding port, and extruding to obtain an intermediate material;
(4) dissolving chitosan in 5-50 times of citric acid, mixing, adding the intermediate material into the mixed solution, taking out the intermediate material after the mixed solution is adhered to the intermediate material, and drying to obtain the feed.
Further, the temperature of the front zone of the twin-screw extruder in the step (3) is 50-90 ℃, the temperature of the rear zone is 110-130 ℃, and the rotating speed is 50-90 HZ.
Further, the chitosan in step (4) has a content of 50000-100000; the mass of the chitosan is 1-10% of the feed.
The sturgeon juvenile fish can not snatch the fed food all the time, but slowly explore the food, feel the food by palpus, then stretch the mouth with large elasticity into a cylinder shape to suck the food into the mouth, and the sturgeon juvenile fish always in a swimming state is easy to scatter the feed after frequently contacting the food, thus causing the waste of the feed. Aiming at the special feeding mode of sturgeon parrs, the problem of stability of the feed for the parrs in water is very critical, the loss rate of nutrient components of the feed is determined by the stability of the feed in water, and the loss of the nutrient components not only can influence the growth of the sturgeon parrs, but also can increase the damage to the breeding environment.
Compared with the prior art, the invention has the following advantages:
the feed for improving the survival rate of the juvenile sturgeon is prepared from the plant composition, has an antibacterial effect, is not easy to decompose in water by coating internal materials with chitosan, prevents the feed from being wasted, and enables the juvenile sturgeon to fully absorb nutritional ingredients, so that the survival rate of the juvenile sturgeon is improved.
Detailed Description
Unless defined otherwise, technical terms used in the following examples have the same meanings as commonly understood by one of ordinary skill in the art to which the present invention belongs. The test reagents used in the following examples, unless otherwise specified, are all conventional biochemical reagents; the experimental methods are conventional methods unless otherwise specified.
The present invention will be described in detail with reference to examples.
Example 1
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 32. The method for preparing the dianthus chinensis extract comprises the following steps: drying and grinding the dianthus chinensis into powder, then putting the powder into 20 times of 60% ethanol, leaching for 2 hours, repeating the leaching for 3 times, then filtering, combining the filtrates, and concentrating to obtain the dianthus chinensis extract. The bombyx batryticatus extract is prepared by the method comprising the following steps: grinding the white muscardine silkworm into powder, putting the powder into 10 times of water, heating for 60 minutes at 80 ℃, then filtering to obtain supernatant, concentrating the supernatant, adding 10 times of absolute ethyl alcohol, standing for 12 hours at 5 ℃, centrifuging, and drying precipitate to obtain the white muscardine silkworm extract.
The preparation method of the feed for improving the survival rate of the juvenile sturgeon comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) uniformly mixing the plant extract composition and the full-fat puffed soybean powder to obtain a premix B;
(3) adding the premix A into a main feeding port of a double-screw extruder, adding the premix B into a side feeding port, and extruding, wherein the temperature of the front zone of the double-screw extruder is 50-90 ℃, the temperature of the rear zone is 110-;
(4) dissolving chitosan in citric acid 20 times the amount of chitosan, mixing, adding the intermediate material into the mixed solution, taking out the intermediate material after the intermediate material is stained with the mixed solution, and drying to obtain the feed. The component of the chitosan in the step (4) is 80000; the mass of the chitosan is 2% of that of the feed.
Example 2
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 60. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Example 3
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 8 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 60. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 1
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 5. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 2
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 120. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 3
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition is a carnation extract. The preparation method of the extract of the dianthus chinensis is the same as that of example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 4
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition is a white muscardine silkworm extract. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 5
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil and 0.2 part of compound vitamin.
The preparation method of the feed for improving the survival rate of the juvenile sturgeon comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) the full-fat puffed soybean flour is premix B;
(3) adding the premix A into a main feeding port of a double-screw extruder, adding the premix B into a side feeding port, and extruding, wherein the temperature of the front zone of the double-screw extruder is 50-90 ℃, the temperature of the rear zone is 110-;
(4) dissolving chitosan in citric acid 20 times the amount of chitosan, mixing, adding the intermediate material into the mixed solution, taking out the intermediate material after the intermediate material is stained with the mixed solution, and drying to obtain the feed. The component of the chitosan in the step (4) is 80000; the mass of the chitosan is 2% of that of the feed.
Comparative example 6
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 20 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 32. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the sturgeon parr is the same as that in the example 1.
Comparative example 7
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil, 0.2 part of compound vitamin and 2 parts of plant extract composition.
The plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 32. The preparation method of the extract of the dianthus chinensis is the same as that of example 1. The preparation method of the bombyx batryticatus extract is the same as that in example 1.
The preparation method of the feed for improving the survival rate of the juvenile sturgeon comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) uniformly mixing the plant extract composition and the full-fat puffed soybean powder to obtain a premix B;
(3) and (2) putting the premix A into a main feeding port of a double-screw extruder, then putting the premix B into a side feeding port, and extruding to obtain the feed, wherein the temperature of the front zone of the double-screw extruder is 50-90 ℃, the temperature of the rear zone is 110-130 ℃, and the rotating speed is 50-90 HZ.
Comparative example 8
The feed for improving the survival rate of the juvenile sturgeon comprises the following raw materials in parts by weight: the feed is prepared from the following raw materials in parts by weight: 18 parts of white fish meal, 42 parts of full-fat expanded soybean meal, 0.2 part of yeast, 15 parts of liver meal, 15 parts of scallop meal, 15 parts of cuttlefish viscera powder, 2 parts of fish oil and 0.2 part of compound vitamin.
The preparation method of the feed for improving the survival rate of the juvenile sturgeon comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) the full-fat puffed soybean flour is premix B;
(3) and (2) putting the premix A into a main feeding port of a double-screw extruder, then putting the premix B into a side feeding port, and extruding to obtain the feed, wherein the temperature of the front zone of the double-screw extruder is 50-90 ℃, the temperature of the rear zone is 110-130 ℃, and the rotating speed is 50-90 HZ.
The plant extract compositions obtained in examples 1-2 and comparative examples 1-5 were subjected to bacteriostatic experiments, the obtained plant extract compositions were added to test tubes containing respectively Cellophilus columni, Aeromonas hydrophila, Aeromonas sobria and Bacillus badius, no composition was added as a blank control, each was repeated three times, and the diameters of the bacteriostatic rings were observed after culturing, and the specific results are shown in Table 1.
TABLE 1 results of bacteriostasis
As can be seen from table 1, the plant extract composition containing the dianthus chinensis extract and the bombyx batryticatus extract simultaneously has inhibitory effects on cellulophilis pillared, aeromonas hydrophila, aeromonas sobria and bacillus favus, the bombyx batryticatus extract with a lower content in comparative example 1 and the bombyx batryticatus extract with a higher content in comparative example 2 have certain effects on the bacteriostatic effects of the plant extracts, and the bacteriostatic effect of the dendrocalamus chinensis extract which is not added in comparative example 4 is more significant than that of the bombyx batryticatus extract which is not added in comparative example 3, which indicates that the influence of the bombyx batryticatus extract on the bacteriostatic effect of the composition is greater than that of the dendrocalamus chinensis extract.
The feed prepared in example 1 and comparative example 7 (each 500 pellets) was put into a culture pond, and the dissolution rate of the feed (dissolution rate: dissolution amount/total amount: 100%) was counted at 1 hour, 3 hours, 6 hours, and 24 hours after the putting without placing the cultured fish in the culture pond as a control of the conventional pellet feed (the particle size was similar to the feed prepared in example 1 and comparative example 7), and the specific results are shown in table 2.
TABLE 2 dissolution results
| 1 hour | 3 hours | 6 hours | 24 hours | |
| Example 1 | 5％ | 18％ | 42％ | 77％ |
| Comparative example 7 | 22％ | 53％ | 91％ | 99％ |
| Control group | 34％ | 67％ | 97％ | 99％ |
As shown in table 2, compared with comparative example 7, the feed prepared in example 1 has significantly reduced dissolution rates in 1 hour, 3 hours, 6 hours and 24 hours, which indicates that the dissolution rate of the feed can be effectively reduced by coating the intermediate material with chitosan, and further indicates that the feed can effectively alleviate the breakage caused by collision of young fish after being coated with chitosan; the feed prepared in comparative example 7 was also improved to some extent compared to the control group, indicating that the feed was extruded at a low temperature to improve the dissolution rate of the feed to some extent.
And (3) testing the fish: 1200 Acipenser sinensis juvenile fishes (60-80 g) are temporarily cultured in an aerated water jar for 2 weeks before the test, and then each juvenile fish is injected with columnar cellulose-philic bacteria.
The experimental fish were divided into 12 groups, and the experimental fish were fed with the feeds obtained in examples 1 to 3 and comparative examples 1 to 8, respectively, and the control group was fed with the conventional feed, and the mortality was counted after 1 week, with the results shown in table 3.
TABLE 3 mortality results
| Item | Mortality (%) |
| Example 1 | 12 |
| Example 2 | 7 |
| Example 3 | 11 |
| Comparative example 1 | 21 |
| Comparative example 2 | 29 |
| Comparative example 3 | 40 |
| Comparative example 4 | 36 |
| Comparative example 5 | 48 |
| Comparative example 6 | 28 |
| Comparative example 7 | 37 |
| Comparative example 8 | 56 |
| Control group | 61 |
As can be seen from table 3, the feed obtained in examples 1 to 3 significantly improved the survival rate of young chinese sturgeons, and the added plant extract composition significantly affected the success rate of young chinese sturgeons, and the chitosan film-forming treatment performed during the feed preparation process also affected the survival rate of young chinese sturgeons.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (9)
1. The feed for improving the survival rate of young sturgeons is characterized in that: the feed is prepared from the following raw materials in parts by weight: 10-20 parts of white fish meal, 30-50 parts of full-fat expanded soybean meal, 0.1-5 parts of yeast, 10-20 parts of liver meal, 10-20 parts of scallop powder, 10-20 parts of cuttlefish viscera powder, 1-10 parts of fish oil, 0.1-1 part of compound vitamin and 1-10 parts of plant extract composition.
2. The feed for improving the survival rate of young sturgeon according to claim 1, characterized in that: the feed is prepared from the following raw materials in parts by weight: 15-20 parts of white fish meal, 40-50 parts of full-fat expanded soybean meal, 0.1-5 parts of yeast, 12-20 parts of liver meal, 12-20 parts of scallop powder, 12-20 parts of cuttlefish viscera powder, 1-10 parts of fish oil, 0.1-1 part of compound vitamin and 1-5 parts of plant extract composition.
3. The feed for improving the survival rate of young sturgeon according to claim 2, characterized in that: the plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 10-100.
4. The feed for improving the survival rate of young sturgeon according to claim 3, characterized in that: the plant extract composition contains the extract of the pink and the extract of the white muscardine silkworm in a mass ratio of 1: 25-65.
5. The feed for improving the survival rate of young sturgeon according to claim 4, characterized in that: the method for preparing the dianthus chinensis extract comprises the following steps: drying and grinding the dianthus chinensis into powder, then putting the powder into 20-30 times of 50-80% ethanol, leaching for 1-3 hours, repeatedly leaching for 3-5 times, then filtering, combining filtrates, and concentrating to obtain the dianthus chinensis extract.
6. The feed for improving the survival rate of young sturgeon according to claim 4, characterized in that: the bombyx batryticatus extract is prepared by the method comprising the following steps: grinding the white muscardine silkworm into powder, putting the powder into 10-20 times of water, heating for 30-60 minutes at 60-90 ℃, then filtering to obtain supernatant, concentrating the supernatant, adding 10-20 times of absolute ethyl alcohol, standing for 6-24 hours at 2-10 ℃, centrifuging, and drying precipitate to obtain the white muscardine silkworm extract.
7. The method for preparing a feed for increasing the survival rate of young sturgeon according to any one of claims 1 to 6, characterized in that: the method comprises the following steps:
(1) uniformly mixing white fish meal, yeast, liver powder, scallop powder, cuttlefish viscera powder, fish oil and compound vitamins to obtain premix A;
(2) uniformly mixing the plant extract composition and the full-fat puffed soybean powder to obtain a premix B;
(3) adding the premix A into a main feeding port of a double-screw extruder, adding the premix B into a side feeding port, and extruding to obtain an intermediate material;
(4) dissolving chitosan in 5-50 times of citric acid, mixing, adding the intermediate material into the mixed solution, taking out the intermediate material after the mixed solution is adhered to the intermediate material, and drying to obtain the feed.
8. The preparation method of the feed for improving the survival rate of the juvenile sturgeon according to claim 7, characterized by comprising the following steps: the temperature of the front zone of the double-screw extruder in the step (3) is 50-90 ℃, the temperature of the rear zone is 110-130 ℃, and the rotating speed is 50-90 HZ.
9. The preparation method of the feed for improving the survival rate of the juvenile sturgeon according to claim 7, characterized by comprising the following steps: the chitosan in the step (4) has the components of 50000-100000; the mass of the chitosan is 1-10% of the feed.
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