CN112707017B - Insulation can for separation heart experiments - Google Patents
Insulation can for separation heart experiments Download PDFInfo
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- CN112707017B CN112707017B CN202110019983.3A CN202110019983A CN112707017B CN 112707017 B CN112707017 B CN 112707017B CN 202110019983 A CN202110019983 A CN 202110019983A CN 112707017 B CN112707017 B CN 112707017B
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- heat preservation
- top cover
- box body
- liquid
- electrode
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D43/00—Lids or covers for rigid or semi-rigid containers
- B65D43/02—Removable lids or covers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
- B65D81/22—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient in moist conditions or immersed in liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/38—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation
- B65D81/3813—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container being in the form of a box, tray or like container
- B65D81/382—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents with thermal insulation rigid container being in the form of a box, tray or like container provided with liquid material between double walls
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D85/00—Containers, packaging elements or packages, specially adapted for particular articles or materials
- B65D85/50—Containers, packaging elements or packages, specially adapted for particular articles or materials for living organisms, articles or materials sensitive to changes of environment or atmospheric conditions, e.g. land animals, birds, fish, water plants, non-aquatic plants, flower bulbs, cut flowers or foliage
Abstract
The invention discloses an incubator for in-vitro heart experiments, which comprises a box body, a top cover and a front cover, wherein the top cover is detachably connected to the top of the box body; the box includes curb plate and bottom plate, all be equipped with the inner chamber in curb plate and the bottom plate, inner chamber in the curb plate and the inner chamber in the bottom plate communicate each other and form the closed heat preservation chamber of integral type, be equipped with heat preservation liquid in the heat preservation chamber, be equipped with the liquid pipe that leads to that is linked together with the heat preservation chamber on the curb plate, it sets up two to lead to the liquid pipe, is the entry and the export of heat preservation liquid respectively. The invention arranges the heat preservation cavity in the box body, continuously circulates the environment-friendly heat preservation liquid in the heat preservation cavity, creates a constant temperature environment in the box body, and ensures the isolated heart to be in a constant temperature state in the experimental process by matching with the perfusion device, thereby ensuring the accuracy of the experimental result.
Description
Technical Field
The invention relates to the technical field of experimental equipment, in particular to an incubator for an in-vitro heart experiment.
Background
An ex vivo cardiac perfusion model for mammals was established by Oscar Langendorff in 1895. The isolated heart perfusion method is widely applied to physiological, pathophysiological and pharmacological researches, so far, people still use the important technology in the field of heart vessels, but in the process of establishing an isolated heart perfusion model, the injury of various operations to cardiac muscle, the great difference between the external environment and the internal environment and the lack of the regulation effect of normal nerve body fluid inevitably damage the cardiac muscle tissue to influence the accuracy of the experiment, or the implementation of certain operations is limited due to narrow space.
The existing widely used heart perfusion system generally only heats perfusion liquid without a heart heat preservation tank, so that the heart is exposed in the air, the temperature maintenance of the heart is not facilitated, and the surface of the heart is easily dried. Or a double-layer heat preservation groove in a beaker shape is used in an experiment, but the volume is small, so that various recording electrodes are not convenient to place, and the heat preservation groove is generally required to be removed in the experiment operation process, so that the heart can not be ensured to keep a good physiological state all the time. The invention provides an incubator for an in-vitro heart experiment, which solves the problems.
Disclosure of Invention
The invention provides an insulation can for isolated heart experiments, which is used with a heart perfusion device and insulation liquid for various isolated heart experiments, so that the heart is under constant temperature and humidity, and the experiment effect is ensured.
The technical scheme adopted by the invention for solving the technical problems is as follows:
an insulation can for in-vitro heart experiments comprises a box body, a top cover and a front cover, wherein the top cover is detachably connected to the top of the box body, and the front cover is detachably connected to the front side of the box body;
the box includes curb plate and bottom plate, all be equipped with the inner chamber in curb plate and the bottom plate, inner chamber in the curb plate and the inner chamber in the bottom plate communicate each other and form the closed heat preservation chamber of integral type, be equipped with heat preservation liquid in the heat preservation chamber, be equipped with the liquid pipe that leads to that is linked together with the heat preservation chamber on the curb plate, it sets up two to lead to the liquid pipe, is the entry and the export of heat preservation liquid respectively.
Furthermore, the top cover is provided with an experimental electrode, the experimental electrode comprises an electrode interface and an electrode, the lead end of the electrode is connected to the electrode interface, the electrode end penetrates through the top cover to be connected to the heart in vitro, and the electrode interface is fixedly connected to the surface of the top cover.
Furthermore, the top cover is provided with heart through holes and electrode through holes, the heart through holes are formed in the middle of the top cover, and the electrode through holes are arranged on the top cover at intervals.
Furthermore, a liquid blocking strip is arranged on a bottom plate of the box body and arranged on the front side of the upper surface of the bottom plate, a perfusion liquid outlet pipe is arranged on the outer side of the liquid blocking strip, a perfusion liquid outlet hole is formed in the perfusion liquid outlet strip, and the perfusion liquid outlet hole is communicated with the perfusion liquid outlet pipe.
Furthermore, the box front side is equipped with the draw-in groove, the draw-in groove sets up two, is located the left and right sides of box leading flank bottom respectively, the protecgulum erects on two draw-in grooves.
Further, a handle is arranged on the top cover and is arranged on the rear side of the top cover.
Further, the temperature of the heat preservation liquid in the heat preservation cavity is not higher than 45 ℃.
Preferably, the box body, the top cover and the front cover are all transparent organic glass.
Preferably, the liquid blocking strip and the clamping groove are connected to the box body through gluing.
The invention has the following beneficial effects:
the heat preservation cavity is arranged in the box body, the environment-friendly liquid is continuously circulated in the heat preservation cavity, a constant-temperature environment is created in the box body, and the isolated heart is always in a constant-temperature state in the experimental process by matching with the perfusion device, so that the accuracy of the experimental result is ensured;
different holes are designed on the top cover according to the experimental requirements and are used for the heart and the electrode to pass through; meanwhile, the experimental electrode is fixed on the top cover, so that various electrodes can conveniently enter the heat insulation box body through the holes and can be placed on the surface of the heart, and the stability of recording the heart electrical signals is ensured;
the front cover is arranged to be movable, and is opened when experimental operation is carried out, so that the experimental operation is convenient; after the cover is closed, the isolated heart can be ensured to work under the relatively constant temperature and humidity environment, so that the stability of experimental conditions is ensured.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is an exploded view of the present invention;
FIG. 3 is a schematic structural diagram of the case of the present invention;
FIG. 4 is a schematic cross-sectional view of the case structure of the present invention;
fig. 5 is a schematic view of the structure of the top cover of the present invention.
Reference numerals: 1-box body, 11-heat preservation cavity, 12-liquid through pipe, 13-liquid blocking strip, 14-clamping groove, 2-top cover, 21-experimental electrode, 22-heart through hole, 23-electrode through hole and 3-front cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the specification, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
As shown in fig. 1, 2, 3 and 4, an incubator for in vitro heart experiments comprises a box body 1, a top cover 2 and a front cover 3, wherein the top cover 2 is detachably connected to the top of the box body 1, and the front cover 3 is detachably connected to the front side of the box body 1; the box body 1 comprises a side plate and a bottom plate, inner cavities are arranged in the side plate and the bottom plate, the inner cavities in the side plate and the bottom plate are communicated with each other to form an integrated closed heat preservation cavity 11, heat preservation liquid is arranged in the heat preservation cavity 11, a liquid through pipe 12 communicated with the heat preservation cavity 11 is arranged on the side plate, and the liquid through pipe 12 is provided with two inlets and outlets for the heat preservation liquid.
The principle of the invention is as follows: be equipped with the inner chamber in the curb plate of box 1 and the bottom plate to formed integrative closed heat preservation chamber 11, sustained cycle fills the thermal insulation liquid of filling constant temperature in heat preservation chamber 11, because heat preservation chamber 11 is located the position of the left and right rear sides of box 1 and bottom surface, make the inside of box maintain under the temperature of relative invariant always, guarantee that the external condition of separation heart experiment meets the requirements. The heart which has been subjected to the isolated perfusion is put into the interior of the box body 1 through the heart through hole 22 on the top cover 2, and the isolated heart is in a relatively stable constant temperature and humidity state; experimenters can open protecgulum 3 and carry out all kinds of experiments to the isolated heart, and can not lead to the experimental result to have great error because of the humiture change of isolated heart.
As shown in fig. 2 and 5, further, an experimental electrode 21 is arranged on the top cover 2, the experimental electrode 21 includes an electrode interface and an electrode, a lead end of the electrode is connected to the electrode interface, the electrode end passes through the top cover 2 and is connected to the isolated heart, and the electrode interface is fixedly connected to the surface of the top cover 2. The electrode interface is used for being connected with the signal amplifier and transmitting an electric signal; the electrodes include recording, stimulating and suction electrodes and are connected to the isolated heart through the various electrodes on the cap 2 via the holes 23.
As shown in fig. 5, the top cover 2 is further provided with a heart passage hole 22 and electrode passage holes 23, the heart passage hole 22 is provided at the center of the top cover 2, the heart which has been subjected to the extracorporeal perfusion enters the incubator, and the electrode passage holes 23 are provided at intervals on the top cover 2.
Preferably, the electrode passage holes 23 comprise a plurality of holes of different apertures for passage of different electrodes.
As shown in fig. 3 and 4, a bottom plate of the box body 1 is provided with a liquid blocking strip 13, the liquid blocking strip 13 is arranged at the front side of the upper surface of the bottom plate, a perfusion liquid outlet pipe is arranged at the outer side of the liquid blocking strip 13, a perfusion liquid outlet hole is arranged in the perfusion liquid blocking strip, and the perfusion liquid outlet hole is communicated with the perfusion liquid outlet pipe. The liquid blocking strip 13 is matched with the perfusion liquid outlet pipe for use, wherein the liquid blocking strip 13 is used for blocking the perfusion liquid from directly flowing out, so that a certain amount of perfusion liquid is reserved on the bottom plate of the box body 1, the constant humidity of the inner cavity of the box body is ensured through evaporation of the perfusion liquid, and meanwhile, the excessive perfusion liquid is discharged through the perfusion liquid outlet pipe, so that a large amount of storage of the perfusion liquid is avoided.
As shown in fig. 3 and 4, two L-shaped slots 14 are provided in the front side of the box 1, and are respectively located on the left side and the right side of the bottom of the front side of the box 1, and the front cover 3 is erected on the two slots 14. In the process of experimental record, the front cover 3 is erected on the clamping groove 14 to seal the interior of the box body, and the constant temperature and humidity are maintained in the box body.
Further, a handle is arranged on the top cover 2 and is arranged on the rear side of the top cover 2. The handle is used for taking and placing the top cover 2.
Further, the temperature of the insulating liquid in the insulating cavity 11 is not higher than 45 ℃.
Preferably, the box body 1, the top cover 2 and the front cover 3 are all transparent organic glass; the liquid blocking strip and the clamping groove are connected to the box body through gluing.
The using method of the invention is as follows: the liquid through pipe 12 is connected with a heat preservation liquid conveying device by using a hose to circulate the heat preservation liquid, so that the inner cavity of the box body 1 is maintained in a constant temperature state; the heart for ex vivo langendorff perfusion is fed into the tank 1 through the heart opening 22 in the center of the lid 2; connecting an experimental electrode 21 with an instrument through an electrode interface, sequentially penetrating the electrode through corresponding electrode through holes 23 to extend into the box body, then opening the front cover 3 to connect the electrode with the isolated heart, and closing the front cover 3 after connection is completed, so that the interior of the box body is relatively closed and is maintained at relatively constant temperature and humidity; when an experiment is needed, the front cover 3 is opened to carry out corresponding experiment operation.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.
Claims (5)
1. An incubator for in-vitro heart experiments is characterized by comprising a box body (1), a top cover (2) and a front cover (3), wherein the top cover (2) is detachably connected to the top of the box body (1), and the front cover (3) is detachably connected to the front side of the box body (1);
the box body (1) comprises a side plate and a bottom plate, inner cavities are arranged in the side plate and the bottom plate, the inner cavities in the side plate and the bottom plate are mutually communicated to form an integrated closed heat preservation cavity (11), heat preservation liquid is arranged in the heat preservation cavity (11), a liquid through pipe (12) communicated with the heat preservation cavity (11) is arranged on the side plate, and the two liquid through pipes (12) are respectively an inlet and an outlet of the heat preservation liquid;
an experimental electrode (21) is arranged on the top cover (2), the experimental electrode (21) comprises an electrode interface and an electrode, the lead end of the electrode is connected to the electrode interface, the electrode end penetrates through the top cover (2) to be connected to the isolated heart, and the electrode interface is fixedly connected to the surface of the top cover (2);
the top cover (2) is provided with heart through holes (22) and electrode through holes (23), the heart through holes (22) are formed in the middle of the top cover (2), and the electrode through holes (23) are arranged on the top cover (2) at intervals;
the top cover (2) is provided with a handle, and the handle is arranged on the rear side of the top cover (2);
the box body (1), the top cover (2) and the front cover (3) are all transparent organic glass.
2. The incubator of claim 1, wherein: be equipped with on the bottom plate of box (1) and keep off liquid strip (13), the front side at the bottom plate upper surface is established in fender liquid strip (13), the liquid strip (13) outside is equipped with the perfusion liquid outlet pipe, and inside is equipped with the perfusion liquid outlet, perfusion liquid outlet and perfusion liquid outlet pipe intercommunication.
3. The incubator of claim 2, wherein: the front side of the box body (1) is provided with two clamping grooves (14), the two clamping grooves (14) are respectively positioned at the left side and the right side of the bottom of the front side face of the box body (1), and the front cover (3) is erected on the two clamping grooves (14).
4. The incubator of claim 1, wherein: the temperature of the heat preservation liquid in the heat preservation cavity (11) is not higher than 45 ℃.
5. The incubator of claim 3, wherein: the liquid blocking strip (13) and the clamping groove (14) are connected to the box body (1) through gluing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110019983.3A CN112707017B (en) | 2021-01-07 | 2021-01-07 | Insulation can for separation heart experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110019983.3A CN112707017B (en) | 2021-01-07 | 2021-01-07 | Insulation can for separation heart experiments |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112707017A CN112707017A (en) | 2021-04-27 |
| CN112707017B true CN112707017B (en) | 2022-02-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110019983.3A Active CN112707017B (en) | 2021-01-07 | 2021-01-07 | Insulation can for separation heart experiments |
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| Country | Link |
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| CN (1) | CN112707017B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201394094Y (en) * | 2009-04-23 | 2010-02-03 | 首都医科大学 | Device for small-scale test animal perfusion fixation |
| CN105277592A (en) * | 2015-09-30 | 2016-01-27 | 山东大学 | Temperature-control detachable bath for configuration of field stimulating electrode and application method thereof |
| CN112106766A (en) * | 2020-11-18 | 2020-12-22 | 中山大学附属第一医院 | Combined perfusion system and perfusion method for isolated heart and lung |
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| US8419729B2 (en) * | 2001-12-04 | 2013-04-16 | Endoscopic Technologies, Inc. | Cardiac ablation devices and methods |
| CA2722615C (en) * | 2008-04-22 | 2018-01-16 | The Board Of Regents Of The University Of Texas System | Fluidics-based pulsatile perfusion organ preservation device |
| CN201727504U (en) * | 2009-06-17 | 2011-02-02 | 河北医科大学 | Synchronous tester for isolated heart physiological characteristics |
| US20110294108A1 (en) * | 2010-05-28 | 2011-12-01 | Tyco Healthcare Group Lp | System and Method for an Ex Vivo Body Organ Electrosurgical Research Device |
| CN106423330B (en) * | 2016-10-08 | 2019-04-09 | 浙江大学 | A kind of experimental heating apparatus |
| CN107014719B (en) * | 2017-03-29 | 2019-07-16 | 河南科技大学 | A kind of experimental box for AGP test experiment |
| CN107894490A (en) * | 2017-10-11 | 2018-04-10 | 孙培培 | A kind of in vitro formula cardiac perfusion device |
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2021
- 2021-01-07 CN CN202110019983.3A patent/CN112707017B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201394094Y (en) * | 2009-04-23 | 2010-02-03 | 首都医科大学 | Device for small-scale test animal perfusion fixation |
| CN105277592A (en) * | 2015-09-30 | 2016-01-27 | 山东大学 | Temperature-control detachable bath for configuration of field stimulating electrode and application method thereof |
| CN112106766A (en) * | 2020-11-18 | 2020-12-22 | 中山大学附属第一医院 | Combined perfusion system and perfusion method for isolated heart and lung |
Non-Patent Citations (1)
| Title |
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| 离体大鼠心脏缺血再灌后冠脉流出液中cTnT与LDH比较研究;来丽娜等;《长治医学院学报》;20091015(第05期);第9-11页 * |
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| CN112707017A (en) | 2021-04-27 |
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