CN201394094Y - Device for small-scale test animal perfusion fixation - Google Patents
Device for small-scale test animal perfusion fixation Download PDFInfo
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- CN201394094Y CN201394094Y CN200920152007XU CN200920152007U CN201394094Y CN 201394094 Y CN201394094 Y CN 201394094Y CN 200920152007X U CN200920152007X U CN 200920152007XU CN 200920152007 U CN200920152007 U CN 200920152007U CN 201394094 Y CN201394094 Y CN 201394094Y
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Abstract
The utility model provides a device for small-scale test animal perfusion fixation, comprising a bracket, a liquid holding device, a liquid conveying pipeline, a T-pipe, a liquid selecting/switching device, a flow speed regulating device and a puncture device. With the device, firstly, sufficient physiological saline is selected by using the liquid selecting/switching device to flush the blood onthe whole body of the experimental animals fixed on test beds; then fixation liquid is selected/switched to finish a systemic circulation and pulmonary circulation along the blood vessel of the wholebody after endocardial perfusion to then return to the right ventricle, therefore, the fixed brain tissue is obtained. The brain dipped by the fixation liquid has certain rigidness; besides, all the tissue forms are maintained in an animation state. Therefore, the device for small-scale test animal perfusion fixation has essential roles on maintaining the form and structure of the brain tissue aswell as the completeness of whole brain by endocardial perfusion fixation method.
Description
Technical field
This utility model relates to a kind of laboratory animal fixed apparatus, particularly relates to a kind ofly being used for the small test animal and pouring into fixed apparatus.
Background technology
The perfusion fixation is used very extensive in the research of cerebral ischemia/anoxia relevant disease, and it is that any stripped fixation is all irreplaceable to the getting express developed of nervous tissue's (brain, spinal cord), fixation.There is this fixation of bibliographical information to be applied to morely in the medical experiment (cat, rabbit, Canis familiaris L.) than large animal see.Up to now, use the rare report of this kind fixation for the minimum animal of body weight, corresponding experimental provision is not seen yet and is related to.This device utilizes body weight to have only the 10-20 gram just, and C57BL/6N gene defection type mice is adopted the practical machinery that is used for the animal medicine scientific research that pours into technique for fixing and develop.
21st century harm humans health commonly encountered diseases, frequently-occurring disease in occupy the umber one with the cardiovascular diseases,, be its main pathogenesis all wherein with the final ischemia/anoxia of cerebral tissue, cardiovascular organization that different inducement was caused no matter be former or secondary.Obviously, brain and cardiovascular ischemia/anoxybiotic research just seems extremely urgent.For this reason, we utilize, and C57BL/6N mice circle of Willis (Willis) is congenital to be lacked as (when ischemia/anoxia takes place, cerebral blood flow is supplied compensatory disappearance) this physiology characteristic, its bilateral common carotid arteries and vagus nerve folder were closed 10 minutes, cause whole cerebral tissue ischemia/anoxia completely, then recover blood flow, be perfusion again, treat that animals survived 24 hours ends, continued treatment is 45 minutes in the hyperbaric oxygen chamber of 2 normal atmospheres, then animal is caused death fast and gets brain, carry out subsequently anti-hypoxia/ischemia and the correlational study of hyperbaric oxygentherapy mechanism aspect.
Based on extremely light this biological characteristics of C57BL/6N mice body weight, can infer its cerebral tissue more tiny, tender and lovely (a few approximately gram).The true correctness that has proved that also we infer.The people that basic medical knowledge arranged slightly as can be known, cerebral tissue is positioned at cranial cavity, 12 pairs of cranial nerve roots that its surface is attached thereto in addition cranial cavities of coming in and going out, its periphery is held by some skulls again, says on the physiology, this is the congenital barrier action of cerebral tissue; But this also carries out scientific research and has increased great difficulty for we obtain complete whole brain tissue.Well imagine that careless manipulation is arranged slightly, both can all that has been achieved is spoiled, cause the failure of drawing materials.Also have, after the sacrifice of animal supply of blood flow stopped, cerebral tissue very easily caused the corruption of melting of self at short notice.So when winning cerebral tissue operation guarantee rapidly, quick, to keep the fresh and complete of organizational structure as far as possible.Otherwise the time is of a specified duration slightly, and a series of degeneration, necrosis will take place brain cell, influences true, the reliability of experimental result equally.
Moreover, contain a large amount of biomacromolecules in the blood, wherein be no lack of many endogenic peroxide enzymes.And if phase mutual interference endogenous, external source oxide enzyme that the flushing of the blood in the cerebral tissue is dried quiet, can take place in the superoxide dismutase just (SOD-1) that we the inquire into variation that protein expression takes place in cerebral ischemia/anoxia surely.
Therefore, careful perfusion fixedly is unique feasible objective selection in winning the cerebral tissue experimentation.The purpose of this utility model provides and a kind ofly is used for the small test animal and pours into fixed apparatus.
Summary of the invention
Apparatus of the present utility model comprises support, liquid apparatus for placing, liquid conducting pipes, tee T, liquid selective/switching device shifter, flow velocity regulation device and sting device.Utilize this device, at first select to wash down the blood of the mice whole body that is fixed on the testing stand with the room temperature normal saline of capacity by liquid selective/switching device shifter, after the careful perfusion of selection/switching fixative, finish a body circulation and pulmonary circulation then, then get back to right ventricle along the whole body blood vessel.Therefore, fixative flowed through the animal whole body the institute in a organized way, all organizing all is fixed.Certainly cerebral tissue is no exception.Brain through fixative soaks has had certain degree of hardness.Also that it is all tissue morphologies maintain animation simultaneously.At this moment, we open cranium and get brain and certainly will just seem easy.So, select for use careful perfusion fixation that the integrity of the form of keeping cerebral tissue, structure and even full brain is had crucial meaning.
Description of drawings
Fig. 1 is the sketch map that the small test animal is poured into fixed apparatus that is used for of the present utility model.
The specific embodiment
With reference to accompanying drawing 1, we will further describe the formation and the use of apparatus of the present utility model.
Of the present utility modelly be used for the small test animal and pour into fixed apparatus and comprise:
Support 1: this support is the irony T-shape support with stable base, selects the brandreth with Steady base for use, and purpose maintains the stability of the apparatus, and the different demands of the high visual animal experimental table of frame are adjustable.The support both sides are left and right sides side arms isometric and parallel to the ground, and the arm outboard end is made " V " type hook downwards, and used to hang the liquid apparatus for placing that normal saline and infusion liquid are housed respectively, liquid apparatus for placing and animal horizontal plane are at a distance of 50 centimetres.Its reason has two: the first, calculates formula as can be known according to liquid pressure, and the height of liquid pressure and liquid level is proportionate.Grope repeatedly through the author, liquid level and animal horizontal plane at a distance of 50 centimetres that height produced was hydrostatic just satisfied: flushing liquor, infusion liquid can be transported to animal whole body large, medium and small blood vessels at different levels, and then reach flushing, fixed purpose; The second, the constant structural intergrity that yet can keep blood vessels at different levels of this pressure can not cause by reason of heavy pressure little variation, causes breaking of animal blood vessels, causes the failure of testing.In addition, animal opens breast and liquid is imported operation such as heart and all need carry out under stereomicroscope, in case operation is finished, require flushing, infusion liquid to get ready in advance, to reach the effect of racing against time, heavier is, also can avoid because the replacing of liquid, air is brought in the liquid, and a nearly step is caused the obstruction of blood vessel, can cause the undesirable of perfusion result equally.
Liquid apparatus for placing 2: can select for use two nature of glass to have master scale,, the upper end leaves opening, the lower end is tapered and is the transfusion bottle that diameter is about 1 centimetre, is about 5 centimetres glass tubing, its maintenance suitable for reading is open, communicates with the external world, and is certain hydrostatic to keep.
Liquid conducting pipes 3 and tee T 6: liquid conducting pipes can be made up of three latex tubings, one end of two latex tubings respectively with the liquid apparatus for placing, as transfusion bottle phase close communication, each long 50 centimetres of two latex tubings, the latex tubing other end respectively with two open communication of the tee T 6 of duroplasts quality, the 3rd opening of tee T 6 links to each other with a latex tubing that has transparent suspension kettle, the flow velocity in the time of can observing the liquid perfusion by transparent suspension kettle, so that regulate flow velocity at any time, the control flow.
Liquid selective/switching device shifter 4 and liquid selective/switching device shifter 5: on the latex tubing that liquid selective/switching device shifter is positioned at transfusion bottle is connected, be used to carry out the selection/switching of liquid.
Flow velocity regulation device 7: the flow velocity regulation device is positioned at the below of transparent suspension kettle, can regulate flow velocity, control flow at any time.
Sting device 8: the sting device that will insert animal hearts is designed to 1mm diameter flexible pipe, and its reason has two: at first, animal hearts is minimum; Secondly, too hard device very easily damages heart.
The device use is as follows:
The perfusion of flushing liquor: mice 9 dorsal positions with the modeling success are fixed on the operating-table 10 earlier, both sides liquid holding bottle 2 is equipped with the flushing liquor of room temperature of prior preparation and the fixative of 4 ℃ of preservations, expose animal hearts, in left apex soft plastic pipe 8 ports carefully are inserted into the right auricle, irritate earlier flushing liquor (normal saline), flow speed control is being the best soon.Require to finish in the 10min, in this course, till the scarlet thin out gradually pink colour of color by beginning of visible right auricle effluent, total liquid measure is 30 milliliters.Whole operation is finished under stereoscope.
After the flushing liquor perfusion is finished, close the outstanding folder of spiral shell of homonymy, open the outstanding folder of offside spiral shell, carry out the perfusion of fixative, flow speed control is preceding 1/2 liquid measure flow velocity fast (30 droplets/minute) slightly, back 1/2 slow (20 droplets/minute), its flow speed control is looked into decision by suspension Hu Liusuguan, requires to finish in the 30min.Cut off the right auricle subsequently, extract the puncture cardiac devices, the animal upset is abdomen shape for sleeping in.Cut off skin of head fast, pry open skull, cut off hard, pia mater encephali, win full brain rapidly, split along the center and cut the two parts that equate about being gently open, wash slightly, cerebral tissue is reentered in the fixative, carry out back and fix with normal saline.Treat condition maturity, both can carry out follow-up experimentation.
Claims (2)
1, a kind ofly be used for the small test animal and pour into fixed apparatus, comprising:
Support, this support are the irony T-shape supports with stable base, and the different demands of the high visual animal experimental table of frame are adjustable; The support both sides are left and right sides side arms isometric and parallel to the ground, and the arm outboard end is made " V " type hook downwards, and are used to hang the liquid apparatus for placing that normal saline and infusion liquid are housed respectively;
The liquid apparatus for placing can select for use two nature of glass to have that master scale, upper end leave opening, the lower end is tapered and is the transfusion bottle that diameter is 1 centimetre, long 5 centimetres a glass tubing, and its maintenance suitable for reading is open, communicates with the external world, and is certain hydrostatic to keep;
Liquid conducting pipes, liquid conducting pipes can be made up of three latex tubings, one end of two latex tubings respectively with liquid apparatus for placing close communication mutually, each long 50 centimetres of two latex tubings, two latex tubing other ends respectively with two open communication in upper end of the tee T of duroplasts quality, the lower ending opening of tee T links to each other with a latex tubing that has transparent suspension kettle, the flow velocity in the time of can observing the liquid perfusion by transparent suspension kettle, so that regulate flow velocity at any time, the control flow;
Liquid selective/switching device shifter, be positioned at latex tubing that transfusion bottle is connected on, be used to carry out the selection/switching of liquid;
The flow velocity regulation device is positioned at the below of transparent suspension kettle, can regulate flow velocity, control flow at any time;
Sting device is designed to 1mm diameter flexible pipe with the sting device that inserts animal hearts.
2, according to claim 1ly be used for the small test animal and pour into fixed apparatus, wherein said liquid apparatus for placing and animal horizontal plane are at a distance of 50 centimetres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920152007XU CN201394094Y (en) | 2009-04-23 | 2009-04-23 | Device for small-scale test animal perfusion fixation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920152007XU CN201394094Y (en) | 2009-04-23 | 2009-04-23 | Device for small-scale test animal perfusion fixation |
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| Publication Number | Publication Date |
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| CN201394094Y true CN201394094Y (en) | 2010-02-03 |
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| CN200920152007XU Expired - Fee Related CN201394094Y (en) | 2009-04-23 | 2009-04-23 | Device for small-scale test animal perfusion fixation |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102614030A (en) * | 2012-04-18 | 2012-08-01 | 新疆维吾尔自治区人民医院 | Device for fixing experimental animal during perfusion and application thereof |
| CN103070736A (en) * | 2013-02-27 | 2013-05-01 | 中国人民解放军第三军医大学 | Perfusion fixation device and method for small animal experiment |
| CN103393479A (en) * | 2013-07-17 | 2013-11-20 | 中国人民解放军第三医院 | Perfusion apparatus for small animals |
| CN108514462A (en) * | 2018-06-11 | 2018-09-11 | 新乡医学院 | A kind of mouse perfusion fixing device and its perfusion fixing means |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
-
2009
- 2009-04-23 CN CN200920152007XU patent/CN201394094Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102614030A (en) * | 2012-04-18 | 2012-08-01 | 新疆维吾尔自治区人民医院 | Device for fixing experimental animal during perfusion and application thereof |
| CN102614030B (en) * | 2012-04-18 | 2014-12-17 | 新疆维吾尔自治区人民医院 | Device for fixing experimental animal during perfusion and application thereof |
| CN103070736A (en) * | 2013-02-27 | 2013-05-01 | 中国人民解放军第三军医大学 | Perfusion fixation device and method for small animal experiment |
| CN103393479A (en) * | 2013-07-17 | 2013-11-20 | 中国人民解放军第三医院 | Perfusion apparatus for small animals |
| CN108514462A (en) * | 2018-06-11 | 2018-09-11 | 新乡医学院 | A kind of mouse perfusion fixing device and its perfusion fixing means |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
| CN112707017B (en) * | 2021-01-07 | 2022-02-18 | 首都医科大学 | Insulation can for separation heart experiments |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100203 Termination date: 20120423 |