CN208308876U - A kind of and matching used culture tray of immunocyte culture bag - Google Patents
A kind of and matching used culture tray of immunocyte culture bag Download PDFInfo
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- CN208308876U CN208308876U CN201820321740.9U CN201820321740U CN208308876U CN 208308876 U CN208308876 U CN 208308876U CN 201820321740 U CN201820321740 U CN 201820321740U CN 208308876 U CN208308876 U CN 208308876U
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Abstract
The utility model relates to a kind of and matching used culture trays of immunocyte culture bag, the culture tray includes chassis, baffle and fluid infusion movable support, wherein the baffle is connected with the chassis surrounding and upwardly extends and formed, one end of the fluid infusion movable support is connect by connector with the flapper, the other end of the fluid infusion movable support is provided with transverse baffle, circular hole is provided on the transverse baffle, the chassis is provided with one group of first hole and one group of second hole, and the diameter in first hole is less than the diameter in second hole.Culture tray provided by the utility model provides supporting role for cell culture bags, facilitates its movement, transhipment.Protective effect is provided simultaneously for cell culture bags.And it, which is provided with one, can rotate to the fluid infusion movable support vertical with chassis, facilitate cell fluid infusion.
Description
Technical field
The utility model relates to Cell Biology Experiment clothing arts, and in particular to a kind of mating with immunocyte culture bag
The culture tray used.
Background technique
Immune cell therapy is separated, is trained in vitro by acquisition autologous patient peripheral blood or umbilical cord blood
It supports, amplification, it is final to feed back to tumor patient, have the function that killing tumor cell, adjust patient's autoimmune function.It is immune thin
Born of the same parents treat tumour and are able to extend patient survival, improve life in patients, reduce the adverse reaction of chemicotherapy, while excitation is exempted from
Epidemic disease monitoring reaction, prevents the recurrence and transfer of tumour, is a kind of effective treatment means of current oncotherapy.
The effective percentage that the quality and quantity of immunocyte treat tumor vaccine cells is of great significance.It is initially immune thin
Born of the same parents are mostly used culture bottle and cultivate, but its space is limited, influence cell growth.The culture bottle quantity that high-volume culture needs increases
It is more, it also will increase production and human cost.And immunocyte culture bag closure is strong, volume is big, gas exchange area is wide, can be big
It is big to promote cell Proliferation, shorten incubation time, save manpower and production cost, reduces cell contamination rate.The material of culture bag is
Soft EVA material, it has not been convenient to it is mobile, and it is easy had the article of corner angle to scratch in moving process.Suspension in culture bag is thin
Born of the same parents can be mobile with culture medium, it has not been convenient to observe under the microscope.For immunocyte in fluid infusion, the fluid infusion hole of culture bag need to be with note
Emitter is connected, and the supporter being connected without one with syringe causes inconvenient for operation.
The above-mentioned statement to background technique it is merely for convenience to technical solutions of the utility model (technological means that uses,
The technical issues of solution and the technical effect of generation etc.) deep understanding, and be not construed as recognizing or with any
Form implies that the information constitutes the prior art known to those skilled in the art.
Utility model content
In view of this, the present invention provides a kind of and the matching used culture trays of immunocyte culture bag, are used for
Solve deficiency existing for existing culture bag.
In an embodiment of the utility model, a kind of and matching used culture of immunocyte culture bag is provided
Pallet, the culture tray includes chassis, baffle and fluid infusion movable support, wherein the baffle is connected with the chassis surrounding
And upwardly extend and formed, one end of the fluid infusion movable support is connect by connector with the flapper, the fluid infusion
The other end of movable support is provided with transverse baffle, and circular hole is provided on the transverse baffle, and the chassis is provided with one group
One hole and one group of second hole, the diameter in first hole are less than the diameter in second hole.
In a specific embodiment, the chassis is rectangle, and the fluid infusion movable support includes first support bar
And second support bar, one end of one end of the first support bar and the second support bar are rotationally connected with the chassis respectively
Two long sides on the baffle midpoint, the other end point of the other end of the first support bar and the second support bar
It is not fixedly connected with the transverse baffle.
In a specific embodiment, the first support bar of the fluid infusion movable support and the length of second support bar
Half that is equal and being greater than chassis length.
In a specific embodiment, the connector is configured to that the fluid infusion movable support is allowed to be parallel to
It the first position on chassis and is rotated perpendicular between the second position on chassis, the quantity of the connector is 2, is respectively arranged at
In the first support bar and the second support bar.
In an optional embodiment, the length on the chassis is 30-40cm, width 21-28cm, the fluid infusion
The length of movable support is 25-30cm.
In an optional embodiment, the height of the baffle is 1-3cm.
In an optional embodiment, the transverse baffle is rectangle, width 2-3cm, and its length is greater than
Or the width equal to chassis.
In an optional embodiment, the diameter of the circular hole is 1.9-2.3cm, and is less than the transverse baffle
Width.
In an optional embodiment, the diameter in first hole is 0.8cm-1.5cm.
In an optional embodiment, the quantity in second hole is 3-5, and the diameter in each second hole is
2-4cm。
In conclusion the utility model provides a kind of and matching used culture tray of immunocyte culture bag, packet
The groove that can place cell culture bags is collectively formed in the baffle for including chassis and connecting with chassis surrounding, and is cell culture bags
Supporting role is provided, movement, the transhipment of cell culture bags are facilitated.Meanwhile protective effect is provided for cell culture bags, it prevents from moving
It is easy there is the article of corner angle to scratch during dynamic, reduces the risk of cell infection.Also, culture support provided by the utility model
Disk, which is provided with one, can rotate to the fluid infusion movable support vertical with chassis, be provided with one on the transverse baffle of bracket one end setting
A circular hole for being used to hang syringe facilitates cell fluid infusion to the fluid infusion hole of auxiliary syringe connection culture bag.Finally, this
The chassis for the culture tray that utility model provides is provided with the second hole and the first hole, and the second hole is observed under the microscope for convenient
The growth conditions of cell determine fluid infusion and harvest time；The aperture in the first hole is used for not less than pallet bore dia is obtained in incubator
The gas exchanges of cell progress maximum area.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of culture tray provided by the utility model.
Specific embodiment
The purpose of this utility model and advantage for ease of understanding will now combine the attached drawing of the utility model, to technical solution
It is described in detail.Case study on implementation discussed below is only a part in the utility model, is not construed as practical to this
Novel concrete restriction.
It is to be appreciated that appended attached drawing is not proportionally drawn, and merely to illustrating the basic principle of the utility model
Various features the suitably simplified technique of painting.The specific design feature of the utility model disclosed herein includes for example specific ruler
Very little, direction, position and shape will be partly determined by the specific application and use environment.
Embodiment 1: culture tray provided by the utility model
Fig. 1 is the structural schematic diagram of culture tray provided by the utility model.In the present specification, the upper of Fig. 1 is defined
Side is the top of culture tray, and lower section is the lower section of culture tray.As shown in Figure 1, the utility model provide it is a kind of with it is immune
The matching used culture tray of cell culture bags, culture tray include chassis 1, baffle 2 and fluid infusion movable support 3, wherein baffle 2
It is connected with 1 surrounding of chassis and upwardly extends and formed, one end of fluid infusion movable support 3 is connected by connector 8 and the rotation of baffle 2
It connects, the other end of fluid infusion movable support 3 is provided with transverse baffle 4, and circular hole 5 is provided on transverse baffle 4, and chassis 1 is provided with one
Group the first hole 6 and one group of second hole 7, the diameter of the diameter in the first hole 6 less than the second hole 7.
In a specific embodiment, chassis 1 is rectangle, and fluid infusion movable support 3 includes first support bar 301 and the
Two support rods 302, one end of first support bar 301 and one end of second support bar 302 are rotationally connected with two length on chassis 1 respectively
The other end of the midpoint of baffle 2 on side, the other end of first support bar 301 and second support bar 302 respectively with transverse gear
Plate 4 is fixedly connected.
In a specific embodiment, fluid infusion movable support 3 can be from the position parallel with chassis 1 by connector 8
It sets, turns to the position perpendicular with chassis 1, and rotatably go back to the position parallel with chassis 1.Fluid infusion movable support 3 and bottom
When disk 1 is perpendicular, the circular hole 5 being arranged on transverse baffle 4 is located at the top on chassis 1, should when carrying out fluid infusion to cell culture bags
Circular hole 5, with the fluid infusion hole of auxiliary syringe connection culture bag, facilitates cell fluid infusion to hang syringe.After the completion of fluid infusion,
Fluid infusion movable support 3 is rotated back to the position parallel with chassis 1, and is placed in and continues to cultivate in incubator.
In a specific embodiment, connector 8 is configured to that fluid infusion movable support 3 is allowed to be parallel to chassis 1
First position and rotated perpendicular between the second position on chassis 1.The quantity of connector 8 is 2, is respectively arranged at first
On strut 301 and second support bar 302.
In a specific embodiment, the first support bar 301 of fluid infusion movable support 3 and second support bar 302
Equal length and the half for being greater than 1 length of chassis.When fluid infusion movable support 3 is turned to the position parallel with chassis 1, laterally
Baffle 4 will not fall in the upper of the cell culture bags being placed in culture tray.
Long side (connecting the both sides of the fluid infusion movable support 3) direction of transverse baffle 4 along chassis 1 is defined in the present specification
A length of transverse baffle 4 width, length of the transverse baffle 4 along a length of transverse baffle 4 of the short side direction on chassis 1.At one
In specific embodiment, the width of transverse baffle 4 is 2-3cm.In a preferred embodiment, the length of transverse baffle 4
Degree is of same size with chassis 1, or greater than the width on chassis 1.
In a preferred embodiment, transverse baffle 4 is rectangle, the other end of first support bar 301 and second
The other end of strut 302 is respectively fixedly connected in the midpoint of the short side of transverse baffle 4.
In a specific embodiment, the length on chassis 1 is 30-40cm, width 21-28cm.Fluid infusion activity branch
The length of frame 3 is 25-30cm.
In a specific embodiment, the height of baffle 2 is 1-3cm.The height is baffle 2 from the four of chassis 1
The length upwardly extended in week.Preferably, baffle 2 upwardly extends 1-3cm along the direction vertical with chassis 1.Baffle 2 and chassis 1 are total
The groove of cell culture bags can be placed with formation, and provides supporting role for cell culture bags, facilitate cell culture bags
Mobile, transhipment.Meanwhile protective effect is provided for cell culture bags, it prevents from being easy there is the article of corner angle to scratch in moving process,
Reduce the risk of cell infection.
In a specific embodiment, the diameter of circular hole 5 is 1.9-2.3cm.Preferably, the diameter of the circular hole 5 is small
In the width of transverse baffle 4.
In a specific embodiment, chassis 1 is provided with the first hole 6 and the second hole 7.The wherein diameter in the first hole 6
For 0.8cm-1.5cm；The quantity in the second hole 7 is 3-5, diameter 2-4cm.Wherein, the second hole 7 is used for conveniently under the microscope
The growth conditions for observing cell, determine fluid infusion and harvest time.The aperture in the first hole 6 is straight not less than pallet hole is obtained in incubator
Diameter carries out the gas exchanges of maximum area for cell.
In a specific embodiment, the material of culture tray is preferably the hard plastic material of easy cleaning, can also be with
It is stainless steel material.
Embodiment 2: the culture tray combination cell culture bag culture CD56 in the utility model embodiment 1 is used+Cell
1, Cord blood 50-150ml is taken, is dispensed into the 50ml centrifuge tube for being previously added 10ml Ficoll, density level bands is carried out
Degree centrifugation.2000g is centrifuged 20min, obtains buffy coat.
2, buffy coat is carefully drawn into 50ml centrifuge tube, and it is thin to 40ml washing that NaCl normal saline solution is added
Born of the same parents.300g is centrifuged 10min.It repeats above step 2-3 times, washes off blood platelet as far as possible, it is thin to obtain the single core of purer peripheral blood
Born of the same parents (PBMC).
3, it counts, according to cell concentration 1.5 × 106/ ml kind is in T175 culture bottle.It is observed under inverted microscope, inoculation is thin
Born of the same parents are very bright, in good condition.37 DEG C are placed in, is cultivated in 5% carbon dioxide incubator.
4, after cultivating 3d, microscopically observation cell state, cell fusion degree is up to 80% or more, fluid infusion to 175ml, and 37
DEG C, it is cultivated in 5% carbon dioxide incubator.
5, after cultivating 6 days, cell fusion degree is transferred in culture bag up to 80% or more, by cell from T175 culture bottle.It will
Culture bag is placed in culture tray in embodiment 1, and the fluid infusion movable support for being placed on culture tray one end is rotated to vertical
It is fixed in pallet, 50ml syringe is connected with the inlet of culture bag, is averagely dispensed according to each T175 culture bottle
Into 3 cell culture bags, every bag of fluid infusion to 300ml.Double swerve cell culture tray is come to mix well cell.It is placed in
It 37 DEG C, is cultivated in 5% carbon dioxide incubator.
6, after cultivating 8d, culture tray is taken out, under inverted microscope, is observed by the second hole intermediate on pallet thin
The growing state of born of the same parents.Cell fusion degree carries out fluid infusion up to 80% or more.
According to the method, cell is grown to the 14-21 days, and nutrient solution volume reaches 1200-1800ml harvest cell.It will support
The culture medium obtained in cell culture bags on disk is put in 500ml centrifuge tube, and cell is harvested by centrifugation in 1800rpm, utilizes syringe point
Loaded in NaCl bottles, feed back to patient.
The description that foregoing exemplary embodiment is presented is only to illustrate the technical solution of the utility model, is not intended to
As without missing, also it is not intended to the utility model to be limited to described precise forms.Obviously, the common skill of this field
Art personnel make many changes according to the above instruction and variation is all possible.Selection illustrative embodiments are simultaneously described and are
In order to explain the certain principles and practical application of the utility model, so that others skilled in the art are convenient for reason
Solve, realize and utilize the various illustrative embodiments and its various selection forms and modification of the utility model.This is practical
Novel protection scope is intended to be limited by the appended claims and its equivalents.
Claims (10)
1. a kind of and matching used culture tray of immunocyte culture bag, which is characterized in that the culture tray includes chassis
(1), baffle (2) and fluid infusion movable support (3), wherein the baffle (2) is connected and upwardly extends with the chassis (1) surrounding
And formed, one end of the fluid infusion movable support (3) is rotatablely connected by connector (8) and the baffle (2), and the fluid infusion is living
The other end of dynamic bracket (3) is provided with transverse baffle (4), is provided with circular hole (5) on the transverse baffle (4), the chassis (1)
It is provided with one group of first hole (6) and one group of second hole (7), the diameter of first hole (6) is less than the straight of second hole (7)
Diameter.
2. the according to claim 1 and matching used culture tray of immunocyte culture bag, which is characterized in that the bottom
Disk (1) is rectangle, and the fluid infusion movable support (3) includes first support bar (301) and second support bar (302), described first
One end of support rod (301) and one end of the second support bar (302) are rotationally connected with two long sides on the chassis (1) respectively
On the baffle (2) midpoint, the other end of the first support bar (301) and the second support bar (302) it is another
One end is fixedly connected with the transverse baffle (4) respectively.
3. the according to claim 2 and matching used culture tray of immunocyte culture bag, which is characterized in that the benefit
The first support bar (301) of liquid movable support (3) and the equal length of second support bar (302) and it is greater than chassis (1) length
Half.
4. the according to claim 2 and matching used culture tray of immunocyte culture bag, which is characterized in that the company
Fitting (8) is configured to allow the fluid infusion movable support (3) being parallel to the first position on chassis (1) and perpendicular to chassis
(1) it is rotated between the second position, the quantity of the connector (8) is 2, is respectively arranged at the first support bar (301)
On the second support bar (302).
5. the according to any one of claim 1 to 4 and matching used culture tray of immunocyte culture bag, feature
It is, the length of the chassis (1) is 30-40cm, and width 21-28cm, the length of the fluid infusion movable support (3) is 25-
30cm。
6. the according to any one of claim 1 to 4 and matching used culture tray of immunocyte culture bag, feature
It is, the height of the baffle (2) is 1-3cm.
7. the according to any one of claim 1 to 4 and matching used culture tray of immunocyte culture bag, feature
It is, the transverse baffle (4) is the width that rectangle, width 2-3cm, and its length are greater than or equal to chassis (1).
8. the according to claim 7 and matching used culture tray of immunocyte culture bag, which is characterized in that the circle
The diameter in hole (5) is 1.9-2.3cm, and is less than the width of the transverse baffle (4).
9. the according to any one of claim 1 to 4 and matching used culture tray of immunocyte culture bag, feature
It is, the diameter of first hole (6) is 0.8cm-1.5cm.
10. the according to any one of claim 1 to 4 and matching used culture tray of immunocyte culture bag, special
Sign is that the quantity of second hole (7) is 3-5, and the diameter of each second hole (7) is 2-4cm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820321740.9U CN208308876U (en) | 2018-03-09 | 2018-03-09 | A kind of and matching used culture tray of immunocyte culture bag |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820321740.9U CN208308876U (en) | 2018-03-09 | 2018-03-09 | A kind of and matching used culture tray of immunocyte culture bag |
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| CN208308876U true CN208308876U (en) | 2019-01-01 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113348240A (en) * | 2019-01-04 | 2021-09-03 | 生物技术食品有限公司 | Bioreactor and method for producing adherent cell culture using the same |
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2018
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113348240A (en) * | 2019-01-04 | 2021-09-03 | 生物技术食品有限公司 | Bioreactor and method for producing adherent cell culture using the same |
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