CN103070736A - Perfusion fixation device and method for small animal experiment - Google Patents
Perfusion fixation device and method for small animal experiment Download PDFInfo
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- CN103070736A CN103070736A CN2013100613985A CN201310061398A CN103070736A CN 103070736 A CN103070736 A CN 103070736A CN 2013100613985 A CN2013100613985 A CN 2013100613985A CN 201310061398 A CN201310061398 A CN 201310061398A CN 103070736 A CN103070736 A CN 103070736A
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Abstract
The invention relates to a perfusion fixation device and a perfusion fixation method for a small animal experiment. The device comprises a pedestal, wherein the upper end face of the pedestal is provided with a first syringe fixing seat, a second syringe fixing seat, a first guide sliding chute and a second guide sliding chute; the first guide sliding chute corresponds to the first syringe fixing seat; the second guide sliding chute corresponds to the second syringe fixing seat; a first slide block is arranged in the first guide sliding chute in a sliding fit manner; a second slide block is arranged in the second guide sliding chute in a sliding fit manner; a first step motor and a second step motor are arranged in the pedestal respectively; the first step motor is connected with the first slide block through a first lead screw nut mechanism; the second step motor is connected with the second slide block through a second lead screw nut mechanism; both the first step motor and the second step motor are electrically connected with a motor control circuit; the pedestal is provided with a perfusion flow speed adjusting key and a motor control switch; and the perfusion flow speed adjusting key and the motor control switch are both electrically connected with the motor control circuit. The device and the method have the advantages of good perfusion effect, convenience in operation and simple structure.
Description
Technical field
The present invention relates to laboratory animal perfusion technique for fixing field, particularly relate to a kind of perfusion fixture and method of toy experiment.
Background technology
In tissues of experimental animals pathological experiment research, particularly carry out in situ hybridization and immunohistochemical study, for avoiding or reducing after death that tissue morphology and structure change, often before sacrifice of animal, adopt the perfusion fixation.Perfusion fixedly is by the blood vessel approach fixative to be imported in the required fixing histoorgan, after the cell of life is in time fixed rapidly in position, wins sample again.The tissue fixing through perfusion has certain hardness, and its all organizational structuries maintain before death state, therefore select careful perfusion fixing be organized in form, keeping of organizational structure integrity has great importance.
The perfusion fixed form can be divided heart and two kinds of perfusions of blood vessel (portal vein, Hepatic artery, femoral artery, ventral aorta etc.) according to the difference of fixative inlet.The organizational structure of each organ is different, can select different infusion liquid inlets, can be from the pulmonary artery entrance from portal vein entrance, lungs such as liver etc.How general large animal is by tremulous pulse, and toy can directly penetrate ventricle or aorta with entry needle, or adopts special device for casting.
The fixing many employing liquid gravity drops of common perfusion pour into fixing, and the multiplex manual injection's of little individual animals method is poured into fixing, up to now, pours into fixture for the minimum animal of body weight and there is no report.Toy such as C57BL/6N and neonate rat body weight light (height 10-15cm, body weight 10-20g), it organizes less, tender and lovely, if fixing bad, will cause the difficulty of drawing materials.In addition, after the sacrifice of animal supply of blood flow stops, organize especially brain and at short notice very easily self-dissolving of myeloid tissue, therefore operation is guaranteed rapid, quick when pouring into fixedly, keeping the fresh and complete of organizational structure as far as possible, otherwise the time is slightly of a specified duration, a series of degeneration, necrosis will occur in brain cell, affect equally verity, reliability and the repeatability of experimental result.
And the easy device that adopts syringe or infusion bottle and entry needle to make up pours into fixingly, also has in practice problems:
(1) the wayward pressure size of hand control syringe: when the perfusion fixation was used the easy device operation of syringe and entry needle combination, pressure was by operator's hand control, and when pressure was too small, perfusion effect was not good; When pressure is excessive, can cause tissue damage (such as angiorrhexis, the heart explosion).When using the easy device operation of infusion bottle and entry needle combination, can be suitable for rat and rabbit, but toy can not be guaranteed perfusion effect because tiny this device pressure of blood vessel is obviously less than normal.
Need simultaneously compounding practice of at least two people when (2) easy device operates: usually need two people compounding practice of above while when the easy device of using syringe and entry needle to make up operates, dissection perfusion, control syringe pressure respectively need a people to finish, and operation is trouble, may cause the imperfect of toy cerebral tissue structure, affect verity, reliability and the repeatability of experimental result.
Summary of the invention
An object of the present invention is the deficiency for the prior art existence, a kind of perfusion fixture of toy experiment is provided, it adopts a motor-driven normal saline flushing, another motor-driven fixative perfusion, perfusion effect is good, easy to operate, simple in structure, and motor is by the full Based Intelligent Control of chip microcontroller, not only can accurately control the pressure of perfusion, and only need people dissection, perfusion to get final product, operation rapidly, fast, time saving and energy saving and high efficiency has widely application.
Another object of the present invention is the deficiency that exists for prior art, a kind of perfusion fixing means that adopts the perfusion fixture of toy experiment is provided, it is by the full Based Intelligent Control of chip microcontroller, not only can accurately control the pressure of perfusion, and can realize the seriality that is connected between the perfusion of normal saline flushing and fixative improving the perfusion fixed effect of toy experiment.
First purpose of the present invention is achieved in that a kind of perfusion fixture of toy experiment, comprise base, described base upper surface is provided with the first syringe holder and the second syringe holder, and it is corresponding with the first syringe holder to be provided with the first guide chute, be provided with the second guide chute corresponding with the second syringe holder, be sliding combined with the first slide block in the first guide chute, be sliding combined with the second slide block in the second guide chute, be respectively equipped with the first motor and the second motor in the described base, described the first motor is connected with the first slide block by the first leading screw and nut mechanism, described the second motor is connected with the second slide block by the second leading screw and nut mechanism, described the first motor, the second motor all is electrically connected circuit for controlling motor, base is provided with perfusion flow rate regulation button and motor control switch, and described perfusion flow rate regulation button and motor control switch all are electrically connected with circuit for controlling motor.
Described circuit for controlling motor comprises power supply, single-chip microcomputer, display circuit, instruction input circuit, the first stepping motor drive ic and the second stepping motor drive ic, described power supply is used for to single-chip microcomputer, display circuit, instruction input circuit, the first stepping motor drive ic and the power supply of the second stepping motor drive ic, and the input and instruction input circuit of described single-chip microcomputer connects; The first outfan of described single-chip microcomputer is connected with the input of the first stepping motor drive ic, and the outfan of described the first stepping motor drive ic is connected with the first motor; The second outfan of described single-chip microcomputer is connected with the input of the second stepping motor drive ic, and the outfan of described the second stepping motor drive ic is connected with the second motor; The 3rd outfan of described single-chip microcomputer is connected with the input of display circuit.
The model of described single-chip microcomputer is STC89C52, and the model of described the first stepping motor drive ic, the second stepping motor drive ic is L293D, and it is the LCD MODULE of LCD1602 that described display circuit adopts model.
Fix respectively the first syringe and the second syringe on described the first syringe holder and the second syringe holder, the front end of two syringes connects a tee T with catheter needle by joint, the first end of described tee T is connected by the front end of joint with the first syringe, the second end of described tee T is connected by the front end of joint with the second syringe, the 3rd end of described tee T is connected with catheter needle by joint, is respectively equipped with switch on first and second end of described tee T.
Be connected with filter by joint between the 3rd end of described tee T and the catheter needle.
Described the first syringe holder and the second syringe holder all comprise front holder and rear holder two parts, the front holder of the first syringe holder and rear holder upper end are equipped with locating slot, and the front holder of the second syringe holder and rear holder upper end are equipped with locating slot.
Described locating slot is semi-cylindrical recesses.
Described base is a casing, and described leading screw and nut mechanism one end is connected by shaft coupling with the output shaft of motor, the leading screw and nut mechanism other end by bearing and seat supports in casing.
Second purpose of the present invention is achieved in that the perfusion fixing means of the perfusion fixture that adopts the toy experiment, has the following steps:
The syringe that 1) syringe of normal saline at first will be housed and fixative is housed is separately fixed on the first syringe holder and the second syringe holder, to be connected with the syringe that fixative is housed with the syringe that normal saline is housed respectively with the tee T of catheter needle, get rid of the threeway inner air tube, set respectively the rate of flooding of normal saline and the rate of flooding of fixative by perfusion flow rate regulation button again;
2) close fixative perfusion switch on the tee T, open normal saline perfusion switch on the tee T, press the motor control switch of control normal saline perfusion, the perfusion normal saline until blood is rinsed well, is closed normal saline perfusion switch on the tee T;
3) fixative of opening tee T pours into switch, presses the motor control switch of control fixative perfusion, pours into fixative by setting rate of flooding, until reach perfusion effect.
The rate of flooding of perfusion fixative adopts principle first quick and back slow, and namely front 10min speed is 4-5ml/min, and rear 10min speed is 2-3ml/min; Rate of flooding reduces the rate of flooding of fixative by regulating perfusion flow rate regulation button in filling process.
Beneficial effect of the present invention is: because perfusion fixture of the present invention adopts the tee T with catheter needle to be connected with the syringe that fixative is housed with the syringe that normal saline is housed respectively, motor is connected with slide block in being slidingly fitted in guide chute by leading screw and nut mechanism, slide block is used for the piston handle of pushing syringe, and the syringe that normal saline is housed is respectively controlled separately by a motor with the syringe that fixative is housed, the rate of flooding that has guaranteed the rate of flooding of normal saline and fixative can be different, and can pour into first and pour into again fixative after normal saline is rinsed the blood in the animal tissue well, avoid blood to affect the verity of experimental result.After the syringe that the syringe of normal saline is housed and fixative is housed being fixed on the holder of base upper surface, the pressure of syringe only needs a people to dissect perfusion and gets final product time saving and energy saving and high efficiency by motor, circuit for controlling motor control.
Two motors of the present invention are by step motor drive chip, Micro Controller Unit (MCU) driving, frequency and the umber of pulse of the pulse signal that the step motor drive chip is accepted only depended in the rotating speed of motor, the position that stops, therefore, the perfusion flow rate regulation button that only needs to operate the instruction input circuit that is connected with single-chip microcomputer can be set respectively the rate of flooding of normal saline and the rate of flooding of fixative, thereby can accurately control injection pressure size and the groundwater increment of injection pressure and the fixative of normal saline, so that perfusion is effective.
And the present invention can also be according to size adjustment injection pressure and the groundwater increment of perfusion animal, and therefore, the present invention not only is practically applicable to the perfusion of mice and neonate rat and fixes, and also is applicable to the bodily form such as rat and fixes than the perfusion of large animal, has widely application.
Be respectively equipped with switch on first and second end of described tee T of the present invention, can prevent the liquid mixed flow, thereby avoid affecting perfusion effect.
Be connected with filter by joint between the 3rd end of described tee T of the present invention and the catheter needle, can filter the impurity in the fixative, guarantee perfusion effect.
The present invention also includes display circuit, can show the duty of rate of flooding and two motors of two syringes.
After the preparation, only need press corresponding motor control switch and can begin to pour into normal saline or fixative, so that the present invention is easy to operate and rapid, quick.In the work process of perfusion normal saline or fixative, at first make the quick perfusion of fixative to whole tissue, reduce subsequently the rate of flooding of fixative by perfusion flow rate regulation button, can prolong fixative arrheaing the time of organizing, fully reach fixing effect.
Description of drawings
Fig. 1 is the schematic diagram of apparatus of the present invention;
Fig. 2 is the structural representation of apparatus of the present invention;
Fig. 3 is that the A of Fig. 2 is to view;
Fig. 4 is that the B-B of Fig. 2 is to cutaway view;
Fig. 5 is the schematic diagram of the circuit for controlling motor of apparatus of the present invention;
Fig. 6 is method flow diagram of the present invention.
In the accompanying drawing, 1 is base, and 2 is the first syringe holder, 3 is the second syringe holder, and 4 is the first guide chute, and 5 is the second guide chute, 6 is the first slide block, and 7 is the second slide block, and 8 is front holder, 9 is rear holder, and 10 is locating slot, and 11 is motor, 12 is leading screw and nut mechanism, and 13 is bearing, and 14 is bearing, 15 is tee T, and 16 is catheter needle, and 17 is the first switch, 18 is second switch, 19 is filter, and 20 is joint, and 21 are perfusion flow rate regulation button, 22 is motor control switch, 23 is the first syringe, and 24 is the second syringe, and 25 is LCDs.
The specific embodiment
Referring to Fig. 1 to Fig. 5, be a kind of embodiment of the perfusion fixture of toy of the present invention experiment.A kind of perfusion fixture of toy experiment, comprise base 1, described base 1 upper surface is provided with the first syringe holder 2 and the second syringe holder 3, and it is corresponding with the first syringe holder 2 to be provided with the first guide chute 4, be provided with the second guide chute 5 corresponding with the second syringe holder 3, be sliding combined with in the first guide chute 4 in the first slide block 6, the second guide chutes 5 and be sliding combined with the second slide block 7.Described the first syringe holder 2 and the second syringe holder 3 all comprise front holder 8 and rear holder 9 two parts, front holder 8 and rear holder 9 upper ends that the front holder 8 of the first syringe holder 2 and rear holder 9 upper ends are equipped with locating slot 10, the second syringe holders 3 are equipped with locating slot 10.Described locating slot 10 is semi-cylindrical recesses.Be respectively equipped with the first motor and the second motor in the described base 1, described the first motor is connected with the first slide block by the first leading screw and nut mechanism, described the second motor is connected with the second slide block by the second leading screw and nut mechanism, described base 1 is a casing, described leading screw and nut mechanism 12 1 ends are connected by shaft coupling with the output shaft of motor 11, and leading screw and nut mechanism 12 other ends are supported in the casing by bearing 13 and bearing 14.
Fix respectively the first syringe 23 and the second syringe 24 on described the first syringe holder 2 and the second syringe holder 3, two syringes 23,24 front end connects a tee T 15 with catheter needle by joint 20, the first end of described tee T 15 is connected with the front end of the first syringe 23 by joint 20, the second end of described tee T 15 is connected with the front end of the second syringe 24 by joint 20, the 3rd end of described tee T 15 is connected with catheter needle 16 by joint 20, first of described tee T 15, be respectively equipped with switch on two ends, being respectively the first switch 17 is that normal saline perfusion switch and second switch 18 are fixative perfusion switch, described the first switch, second switch can prevent the perfusion fluid mixed flow.Be connected with filter 19 by joint between the 3rd end of the described tee T 15 of the present embodiment and the catheter needle 16, can effectively consider the interior foreign body of fixative and avoid affecting the perfused tissue effect.Described catheter needle 16 is syringe needle, and syringe needle removes point, avoids animal hearts is caused accidental injury.Described the first syringe holder 2 and the second syringe holder 3 are one group of syringe holder, the present invention can also arrange many group syringe holders at base 1, and setting guide chute, slide block, leading screw and nut mechanism, the motor corresponding with its syringe holder, control described motor by circuit for controlling motor again, can realize that the same time pours into branched animal, charging efficiency is high.
Described the first motor U5, the second motor U6 all are electrically connected circuit for controlling motor, base 1 is provided with four perfusion flow rate regulation buttons 21 and two motor control switch 22, and described four perfusion flow rate regulation buttons 21 and two motor control switch 22 all are electrically connected with circuit for controlling motor.Described circuit for controlling motor comprises power supply, single-chip microcomputer U1, display circuit, instruction input circuit, the first stepping motor drive ic U2 and the second stepping motor drive ic U3, described power supply is used for to single-chip microcomputer U1, display circuit, instruction input circuit, the first stepping motor drive ic U2 and the second stepping motor drive ic U3 power supply, the input and instruction input circuit of described single-chip microcomputer U1 connects, and is used for receiving the instruction input of instruction input circuit.Described instruction input circuit comprises four perfusion flow rate regulation buttons 21 and four resistance, four perfusion flow rate regulation buttons 21 are respectively the first turbo SB1, the first deceleration button SB2, the second turbo SB3, the second deceleration button SB4, and four resistance are respectively the first resistance R 1, the second resistance R 2, the 3rd resistance R 3, the 4th resistance R 4.Described perfusion flow rate regulation button 21 is used for setting the rate of flooding of normal saline and the rate of flooding of fixative, can accelerate and slow down.The first outfan of described single-chip microcomputer U1 is connected with the input of the first stepping motor drive ic U2, be used for the output pulse control signal to the first stepping motor drive ic U2, the outfan of described the first stepping motor drive ic U2 is connected with the first motor U5, be used for driving the first motor U5 according to pulse control signal, control stroke and the rotary speed of the first motor U5.The second outfan of described single-chip microcomputer U1 is connected with the input of the second stepping motor drive ic U3, be used for the output pulse control signal to the second stepping motor drive ic U3, the outfan of described the second stepping motor drive ic U3 is connected with the second motor U6, be used for driving the second motor U6 according to pulse control signal, control stroke and the rotary speed of the second motor U6.Two motor control switch 22 are respectively the first motor control switch, the second motor control switch, and described the first motor control switch is used for the start and stop of control the first motor between the first motor and power supply.The second motor control switch is used for the start and stop of control the second motor between the second motor and power supply.Described the first motor control switch is the motor control switch of control normal saline perfusion, and described the second motor control switch is the motor control switch of control fixative perfusion.The first motor U5 and the second motor U6 of the present embodiment are four phase step motor, and four phase step motor has five interfaces, is respectively first interface, the second interface, the 3rd interface, the 4th interface, the 5th interface.The 3rd outfan of described single-chip microcomputer U6 is connected with the input of display circuit, be used for serial data and control signal are passed to display circuit, be used for showing the duty of the rate of flooding of the first syringe, the second syringe and the first motor, the second motor.
It is the single-chip microcomputer STC89C52 of U1 that the present embodiment adopts model, XTAL1, the XTAL2 of described U1 are connected with crystal oscillating circuit, described crystal oscillating circuit comprises crystal oscillator X, the first capacitor C 1, the second capacitor C 2, the P3.4 of described U1 is connected with warning circuit, be used for the caution of perfusion flow process when flow process finishes, described warning circuit comprises the 5th resistance R 5, PNP audion VZ1 and attention device Bell.The model of described the first stepping motor drive ic U2 of the present embodiment, the second stepping motor drive ic U3 is L293D, it is the LCD MODULE U4 of LCD1602 that described display circuit adopts model, and the LCDs 25 of described LCD MODULE U4 is installed on the sidewall of base 1.The physical circuit of the present embodiment connects as follows:
Referring to Fig. 5, the P1.0 of described U1 connects the first input IN1 of U2, and the P1.1 of described U1 connects the second input IN2 of U2, and the P1.2 of described U1 connects the 3rd input IN3 of U2, and the P1.3 of described U1 connects the 4th input IN4 of U2; The P1.4 of described U1 connects the first input IN1 of U3, and the P1.5 of described U1 connects the second input IN2 of U2, and the P1.6 of described U1 connects the 3rd input IN3 of U2, and the P1.7 of described U1 connects the 4th input IN4 of U2; The P2.0 of described U1 connects the 7th pin of U4, the P2.1 of described U1 connects the 8th pin of U4, the P2.2 of described U1 connects the 9th pin of U4, the P2.3 of described U1 connects the 10th pin of U4, the P2.4 of described U1 connects the 11st pin of U4, the P2.5 of described U1 connects the 12nd pin of U4, the P2.6 of described U1 connects the 13rd pin of U4, the P2.7 of described U1 connects the 14th pin of U4, the P3.6 of described U1 connects the 4th pin of U4, and the P3.7 of described U1 connects the 6th pin of U4, and the 15th pin of described U4 all connects power supply VCC with the 2nd pin, the 16th pin of described U4 and the equal ground connection of the 1st pin, the 3rd pin of described U4 is by variable resistance R6 ground connection; Described crystal oscillator X is connected on the XTAL1 of U1, the two ends of XTAL2, and the first capacitor C 1 is connected between XTAL1 and the ground, and the second capacitor C 2 is connected between XTAL2 and the ground; Described the first turbo SB1 is connected between the P3.0 and ground of U1, the first deceleration button SB2 is connected between the P3.1 and ground of U1, the second turbo SB3 is connected between the P3.2 and ground of U1, the second deceleration button SB4 is connected between the P3.3 and ground of U1, described the first resistance R 1 is connected between the P3.0 and power supply VCC of U1, the second resistance R 2 is connected between the P3.1 and power supply VCC of U1, the 3rd resistance R 3 is connected between the P3.2 and power supply VCC of U1, and the 4th resistance R 4 is connected between the P3.3 and power supply VCC of U1; Described the 5th resistance R 5 is connected between the base stage of the P3.4 of U1 and PNP audion VZ1, and alarm Bell is connected between the emitter stage and power supply VCC of PNP audion VZ1, the grounded collector of described PNP audion VZ1; The Vcc of described U1 connects power supply VCC, the GND ground connection of described U1.
The first output OUT1 of U2 connects the 5th interface of the first motor U5, the second output OUT2 of U2 connects the 4th interface of the first motor U5, the 3rd output OUT3 of U2 connects the 3rd interface of the first motor U5, the 4th output OUT4 of U2 connects the second interface of the first motor U5, the first interface of the first motor U5 connects power supply VCC, the equal ground connection of GND pin of U2, the VSS of U2, E1 are connected with the E2 pin and are connected power supply VCC, the VS pin of U2 connects power supply 12V, is used for driving U2; The first output OUT1 of U3 connects the 5th interface of the second motor U6, the second output OUT2 of U3 connects the 4th interface of the second motor U6, the 3rd output OUT3 of U3 connects the 3rd interface of the second motor U6, the 4th output OUT4 of U3 connects the second interface of the second motor U6, the first interface of the second motor U6 connects power supply VCC, the equal ground connection of GND pin of U3, the VSS of U3, E1 are connected with the E2 pin and are connected power supply VCC, the VS pin of U3 connects power supply 12V, is used for driving U3.
Referring to Fig. 6, the flow chart for the perfusion fixing means of the perfusion fixture that adopts the toy experiment has the following steps:
Second syringe that 1) the first syringe of normal saline at first will be housed and fixative (4% paraformaldehyde) is housed is separately fixed on the first syringe holder and the second syringe holder, to be connected with the second syringe that fixative is housed with the first syringe that normal saline is housed respectively with the tee T of catheter needle, close the normal saline perfusion switch on the first end of tee T, open the fixative perfusion switch on the second end of tee T, regulate the perfusion flow velocity by perfusion flow rate regulation button and fill fast second of tee T, three ends, then close the fixative perfusion switch on the second end of tee T, open the normal saline perfusion switch on the first end of tee T, until normal saline is from pouring into the normal saline perfusion switch of closing after syringe needle flows out on the tee T first end, get rid of the threeway inner air tube, set respectively the rate of flooding of normal saline and the rate of flooding of fixative by perfusion flow rate regulation button again.Be fixative 20ml in the in-built normal saline 20ml of the first syringe described in the present embodiment, the second syringe.
2) be fixed on the cystosepiment lying on the back again after the Animal Anesthesia, open the thoracic cavity, expose heart, with eye scissors take back at the apex of the heart (left ventricle) cut an osculum, gently thrust reeve ascending aorta from the left apex of the heart according to anatomical structure with catheter needle, after fixing with ophthalmic tweezers, cut off the right auricle, open the normal saline perfusion switch on the first end of tee T, press the first motor control switch of control normal saline perfusion, start the first motor, the perfusion normal saline, until blood rinses well, close the normal saline perfusion switch on the first end of tee T.The speed of the perfusion normal saline that the present embodiment is set is 4-5ml/min.
When 3) treating that liquid that the animal right atrium flows out becomes colorless liquid from scarlet blood, open the fixative perfusion switch on the second end of tee T, press motor second gauge tap of control fixative perfusion, start the second motor, by setting rate of flooding perfusion fixative, until reach perfusion effect, extract catheter needle after reaching perfusion effect, carry out tissue sampling.The rate of flooding of perfusion fixative adopts principle first quick and back slow, and namely front 10min speed is 4-5ml/min, and rear 10min speed is 2-3ml/min; Rate of flooding reduces the rate of flooding of fixative by regulating perfusion flow rate regulation button in filling process.
Apparatus of the present invention and method not only are practically applicable to the perfusion of mice and neonate rat and fix, and also are applicable to the bodily forms such as rat and fix than the perfusion of large animal, have widely application.
Claims (10)
1. the perfusion fixture of toy experiment, comprise base, it is characterized in that: described base upper surface is provided with the first syringe holder and the second syringe holder, and it is corresponding with the first syringe holder to be provided with the first guide chute, be provided with the second guide chute corresponding with the second syringe holder, be sliding combined with the first slide block in the first guide chute, be sliding combined with the second slide block in the second guide chute, be respectively equipped with the first motor and the second motor in the described base, described the first motor is connected with the first slide block by the first leading screw and nut mechanism, described the second motor is connected with the second slide block by the second leading screw and nut mechanism, described the first motor, the second motor all is electrically connected circuit for controlling motor, base is provided with perfusion flow rate regulation button and motor control switch, and described perfusion flow rate regulation button and motor control switch all are electrically connected with circuit for controlling motor.
2. the perfusion fixture of toy according to claim 1 experiment, it is characterized in that: described circuit for controlling motor comprises power supply, single-chip microcomputer, display circuit, instruction input circuit, the first stepping motor drive ic and the second stepping motor drive ic, described power supply is used for to single-chip microcomputer, display circuit, instruction input circuit, the first stepping motor drive ic and the power supply of the second stepping motor drive ic, and the input and instruction input circuit of described single-chip microcomputer connects; The first outfan of described single-chip microcomputer is connected with the input of the first stepping motor drive ic, and the outfan of described the first stepping motor drive ic is connected with the first motor; The second outfan of described single-chip microcomputer is connected with the input of the second stepping motor drive ic, and the outfan of described the second stepping motor drive ic is connected with the second motor; The 3rd outfan of described single-chip microcomputer is connected with the input of display circuit.
3. the perfusion fixture of toy according to claim 2 experiment, it is characterized in that: the model of described single-chip microcomputer is STC89C52, the model of described the first stepping motor drive ic, the second stepping motor drive ic is L293D, and it is the LCD MODULE of LCD1602 that described display circuit adopts model.
4. the perfusion fixture of toy according to claim 1 experiment, it is characterized in that: fix respectively the first syringe and the second syringe on described the first syringe holder and the second syringe holder, the front end of two syringes connects a tee T with catheter needle by joint, the first end of described tee T is connected by the front end of joint with the first syringe, the second end of described tee T is connected by the front end of joint with the second syringe, the 3rd end of described tee T is connected with catheter needle by joint, first of described tee T, be respectively equipped with switch on two ends.
5. the perfusion fixture of toy experiment according to claim 4 is characterized in that: be connected with filter by joint between the 3rd end of described tee T and the catheter needle.
6. according to claim 1 or the perfusion fixture of 4 described toys experiment, it is characterized in that: described the first syringe holder and the second syringe holder all comprise front holder and rear holder two parts, the front holder of the first syringe holder and rear holder upper end are equipped with locating slot, and the front holder of the second syringe holder and rear holder upper end are equipped with locating slot.
7. the perfusion fixture of toy according to claim 1 experiment, it is characterized in that: described locating slot is semi-cylindrical recesses.
8. the perfusion fixture of toy according to claim 1 experiment, it is characterized in that: described base is a casing, described leading screw and nut mechanism one end is connected by shaft coupling with the output shaft of motor, the leading screw and nut mechanism other end by bearing and seat supports in casing.
9. a perfusion fixing means that adopts the perfusion fixture of toy experiment is characterized in that, has the following steps:
The syringe that 1) syringe of normal saline at first will be housed and fixative is housed is separately fixed on claim 1 described the first syringe holder and the second syringe holder, to be connected with the syringe that fixative is housed with the syringe that normal saline is housed respectively with the tee T of catheter needle, get rid of the threeway inner air tube, set respectively the rate of flooding of normal saline and the rate of flooding of fixative by perfusion flow rate regulation button again;
2) close fixative perfusion switch on the tee T, open normal saline perfusion switch on the tee T, press the motor control switch of control normal saline perfusion, the perfusion normal saline until blood is rinsed well, is closed normal saline perfusion switch on the tee T;
3) fixative of opening tee T pours into switch, presses the motor control switch of control fixative perfusion, pours into fixative by setting rate of flooding, until reach perfusion effect.
10. the perfusion fixing means of the perfusion fixture of employing toy according to claim 9 experiment, it is characterized in that: the rate of flooding of perfusion fixative adopts principle first quick and back slow, 10min speed is 4-5ml/min namely, and rear 10min speed is 2-3ml/min; Rate of flooding reduces the rate of flooding of fixative by regulating perfusion flow rate regulation button in filling process.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN107551344A (en) * | 2017-10-13 | 2018-01-09 | 中国人民解放军总医院 | Quick administering device and method |
| CN109596610A (en) * | 2018-12-29 | 2019-04-09 | 莱茵技术（上海）有限公司 | Content of formaldehyde rapid detection method and device in a kind of textile |
| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
| CN112891013A (en) * | 2021-01-27 | 2021-06-04 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal cavity of small rodent experimental animal |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105854661A (en) * | 2016-06-17 | 2016-08-17 | 四川大学 | Automatic mixed medicament preparation device and work method thereof |
| CN105854661B (en) * | 2016-06-17 | 2018-11-13 | 四川大学 | A kind of automatic preparation facilities and its working method of confection |
| CN107551344A (en) * | 2017-10-13 | 2018-01-09 | 中国人民解放军总医院 | Quick administering device and method |
| CN107551344B (en) * | 2017-10-13 | 2023-07-14 | 中国人民解放军总医院 | Rapid drug delivery device and method |
| CN109596610A (en) * | 2018-12-29 | 2019-04-09 | 莱茵技术（上海）有限公司 | Content of formaldehyde rapid detection method and device in a kind of textile |
| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
| CN112891013A (en) * | 2021-01-27 | 2021-06-04 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal cavity of small rodent experimental animal |
| CN112891013B (en) * | 2021-01-27 | 2023-08-25 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal of small rodent experimental animal |
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