CN112616830A - Perfusion fixing system for pathological section - Google Patents
Perfusion fixing system for pathological section Download PDFInfo
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- CN112616830A CN112616830A CN202011585683.3A CN202011585683A CN112616830A CN 112616830 A CN112616830 A CN 112616830A CN 202011585683 A CN202011585683 A CN 202011585683A CN 112616830 A CN112616830 A CN 112616830A
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- transparent
- perfusion
- suspension
- circular
- constant temperature
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B21/00—Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
- F26B21/004—Nozzle assemblies; Air knives; Air distributors; Blow boxes
Abstract
The invention belongs to the technical field of medical instruments, in particular to a perfusion fixing system for pathological sections, which comprises a transparent constant temperature box, wherein the top end of the transparent constant temperature box is rotatably connected with a transparent constant temperature box cover, the transparent constant temperature box cover is provided with a drying fan and an exhaust fan, the short edge at one side of the transparent constant temperature box is provided with a cleaning mechanism, the bottom of the inner wall of the transparent constant temperature box is provided with a suspension supporting mechanism, the short edge at the other side of the transparent constant temperature box is provided with a perfusion fixing mechanism, the isolated kidney can be quickly perfused and fixed through the perfusion fixing mechanism, the whole transparent constant temperature box is filled with four-degree fixing liquid, the temperature of the fixing liquid is constant through a thermostat, the kidney is easy to store, the whole kidney is suspended in the fixing liquid through the suspension supporting mechanism, the influence of the outside on the kidney is avoided, the influence on the, the device is dried by a drying fan and an exhaust fan.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a perfusion fixing system for pathological sections.
Background
The perfusion fixation method is a fixation method adopted when certain tissue blocks are too large in volume or fixing liquid is extremely difficult to permeate into the inside of the tissue blocks or the whole animal body needs to be fixed, the perfusion fixation is that physiological saline is firstly injected into blood vessels, the blood vessels reach the whole tissue or the whole body through the blood vessel branches, blood is discharged, then the fixing liquid is injected into the blood vessels and reaches the whole tissue or the whole body through the blood vessel branches, so that the whole tissue or the organ is sufficiently fixed, in order to preserve the pathological characteristics of the isolated kidney to the maximum extent, the more rapid the fixation of the isolated kidney is, the kidney needs to be fixed by the perfusion fixation method, the perfusion fixation can lead the whole kidney to be fixed more rapidly, the effect is good, the pathology of the kidney can be obtained more accurately, at present, the perfusion fixation device special for the pathological section of the isolated kidney is rarely used, and in the perfusion, the kidney generally receives external oppression, for example the kidney can't suspend in the stationary liquid, and then sinks at the bottom, and this pathological feature that will influence the kidney makes the accuracy of pathologist to kidney pathology's analysis receive the influence.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a perfusion fixing system for pathological sections, which has the characteristics of high perfusion fixing speed and no interference to the pathological features of the kidney.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a fill fixed system for pathological section, includes transparent thermostated container, transparent thermostated container top one side is rotated and is connected with transparent thermostated container lid, transparent thermostated container minor face outer wall one side top-down installs washing liquid injection fill, miniature suction pump, thermostat and catheter, washing liquid injection fill through three-way valve three with transparent thermostated container with miniature suction pump is linked together, miniature suction pump with the thermostat is linked together, the thermostat with the one end of catheter is linked together, the other end and the miniature spray pump of catheter are linked together, miniature spray pump is installed in the bottom central authorities of transparent thermostated container, miniature spray pump is linked together through water spray interface and suspension supporting mechanism, transparent thermostated container minor face outer wall opposite side is installed and is filled fixed establishment, the support is installed to transparent thermostated container bottom, the cleaning device comprises a transparent thermostat, a support, a micro water spray pump, a cleaning solution injection hopper, a cleaning nozzle, a micro water suction pump, a thermostat, a liquid guide pipe and a micro water spray pump, wherein the bottom of the transparent thermostat is provided with a plurality of liquid discharge valves, the liquid discharge valves are positioned between the support and the micro water spray pump, four inner side walls of the transparent thermostat are respectively provided with four cleaning nozzles, the cleaning nozzles are positioned at the geometric center of the inner side walls of the transparent thermostat, the upper part of the inner wall of the transparent thermostat, which is close to one side of the perfusion fixing mechanism, is provided with a soft cleaning interface, and the cleaning solution injection hopper, the soft cleaning interface;
the suspension supporting mechanism comprises a circular chassis box, the circular chassis box is installed in the middle of the bottom of the inner wall of the transparent constant temperature box, the circular chassis box is communicated with the micro water spray pump through the water spray interface, a plurality of arc-shaped supporting tubes are symmetrically connected to the periphery of the circular chassis box, the arc-shaped supporting tubes are connected with the transparent constant temperature box through stabilizing ribs, one ends of the arc-shaped supporting tubes, far away from the circular chassis box, are connected with circular supporting tubes, the circular chassis box, the arc-shaped supporting tubes and the circular supporting tubes are communicated with each other, a plurality of suspension sprayers are connected to the inner sides of the circular chassis box, the arc-shaped supporting tubes and the circular supporting tubes, the circular chassis box, the arc-shaped supporting tubes and the circular supporting tubes are communicated with the suspension sprayers, and the spraying direction of the suspension, the concentration point of the spraying direction of the suspension spray heads on the arc-shaped supporting pipe and the circular supporting pipe is positioned above the circle center of the circular supporting pipe;
the perfusion fixing mechanism comprises an exhaust hole, a peristaltic pump, a miniature negative pressure device and a support frame from top to bottom, the exhaust hole, the peristaltic pump, the miniature negative pressure device and the support frame are all installed on the outer side wall of the transparent constant temperature box, the exhaust hole penetrates through the transparent constant temperature box and is connected with an exhaust hose, one end, far away from the exhaust hole, of the exhaust hose is connected with an exhaust chamber, the peristaltic pump penetrates through the transparent constant temperature box and is connected with a perfusion liquid inlet hose, the perfusion liquid inlet hose is communicated with the exhaust chamber through a three-way valve I, the exhaust chamber is positioned right above the perfusion liquid inlet hose, the top end of the perfusion liquid inlet hose is connected with a perfusion liquid inlet joint, the liquid inlet end of the miniature negative pressure device penetrates through the transparent constant temperature box and is connected with a perfusion liquid outlet hose, the top end of the perfusion, and the one-way valve is close to the perfusion liquid outlet joint, the liquid outlet end of the miniature negative pressure device is connected with a three-way valve II, the other two interfaces of the three-way valve II are respectively connected with a blood temporary storage box and a physiological saline waste liquid temporary storage box, the blood temporary storage box and the physiological saline waste liquid temporary storage box are placed on the support frame, and the perfusion liquid inlet joint is similar to the perfusion liquid outlet joint in structure.
Preferably, the outer wall connection who fills the inlet joint has two sets of anti-skidding archs, fill the inlet joint suit elasticity retainer plate, just the elasticity retainer plate is located between the anti-skidding archs, the internal diameter of elasticity retainer plate equals the external diameter of soft washing interface, the top of filling the inlet joint is the toper structure.
Preferably, the suspension sprayer comprises a suspension sprayer body and a suspension sprayer liquid storage chamber, the suspension sprayer liquid storage chamber is of a conical structure, the funnel end of the suspension sprayer is communicated with the suspension sprayer body, and a plurality of suspension sprayer spray holes which are regularly distributed are formed in the conical bottom end of the suspension sprayer liquid storage chamber.
Preferably, the mid-mounting of transparent constant temperature case lid has drying fan, the exhaust fan is installed to the middle part both sides of transparent constant temperature case lid, drying fan with the exhaust fan runs through transparent constant temperature case lid, one side of transparent constant temperature case lid with transparent constant temperature case rotates to be connected, the opposite side of transparent constant temperature case lid is connected with the handle.
Preferably, the exhaust hole is a circular ring-shaped elastic piece, the inner wall of the exhaust hole is in interference connection with a syringe, and the diameter of the nipple of the syringe is larger than the inner diameter of the exhaust hole.
Preferably, the micro water spray pump is connected with the soft cleaning interface and the cleaning spray head through a valve.
Preferably, the top end of the cleaning spray head is conical, and a plurality of nozzles are arranged at the top end and the side surface of the conical shape.
Compared with the prior art, the invention has the beneficial effects that:
1. the in-vitro kidney can be rapidly perfused and fixed by the perfusion fixing mechanism, the whole transparent constant temperature box is filled with four-degree fixing liquid, and the temperature of the fixing liquid is constant by the thermostat, so that the kidney can be more easily stored;
2. the whole kidney can be suspended in the fixing liquid through the arranged suspension supporting mechanism, so that the influence of the outside on the kidney is avoided, and the influence on the pathological characteristics of the kidney is reduced;
3. the device after using can be washd through the wiper mechanism who sets up, and drying fan and the exhaust fan through setting up can be dried the device after wasing to use next time.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the interior of the transparent incubator according to the present invention;
FIG. 3 is a schematic structural view of a suspension spray head according to the present invention;
FIG. 4 is a schematic view of the structure of the perfusion inlet joint of the present invention;
FIG. 5 is a schematic structural view of a cleaning head according to the present invention;
fig. 6 is a schematic structural view of a transparent thermostat cover according to the present invention.
In the figure: 1. a transparent thermostat; 11. a drain valve; 12. a water spray interface; 2. a transparent constant-temperature box cover; 21. a drying fan; 22. an exhaust fan; 23. a handle; 3. a support; 4. a suspension support mechanism; 41. a circular chassis box; 42. an arc-shaped supporting tube; 43. a suspension nozzle; 431. a suspended nozzle body; 432. a suspension nozzle liquid storage chamber; 433. spraying holes of the suspension spray head; 44. a circular support tube; 45. stabilizing the ribs; 5. a perfusion fixing mechanism; 51. an exhaust hole; 511. an exhaust hose; 512. an exhaust chamber; 513. a three-way valve I; 52. an injector; 53. a peristaltic pump; 531. filling a liquid inlet hose; 532. filling a liquid inlet joint; 5321. anti-skid protrusions; 5322. an elastic fixing ring; 54. a micro negative pressure device; 541. pouring a liquid outlet hose; 542. a one-way valve; 543. a liquid outlet joint is filled; 55. a three-way valve II; 56. a support frame; 561. a blood temporary storage tank; 562. a physiological saline waste liquid temporary storage box; 6. a cleaning mechanism; 61. a cleaning liquid injection hopper; 62. a soft cleaning interface; (ii) a 64. Cleaning the spray head; 65. a valve; 7. a micro water pump; 8. a thermostat; 9. a catheter; 10. a micro water spray pump.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides the following technical solutions: a perfusion fixing system for pathological sections comprises a transparent thermostat 1, one side of the top end of the transparent thermostat 1 is rotatably connected with a transparent thermostat cover 2, one side of the outer wall of the short edge of the transparent thermostat 1 is provided with a cleaning liquid injection hopper 61, a micro water pump 7, a thermostat 8 and a catheter 9 from top to bottom, the cleaning liquid injection hopper 61 is communicated with the transparent thermostat 1 and the micro water pump 7 through a three-way valve three 63, the micro water pump 7 is communicated with the thermostat 8, the thermostat 8 is communicated with one end of the catheter 9, the other end of the catheter 9 is communicated with a micro water spray pump 10, the micro water spray pump 10 is arranged in the center of the bottom of the transparent thermostat 1, the micro water spray pump 10 is communicated with a suspension supporting mechanism 4 through a water spray interface 12, the other side of the outer wall of the short edge of the transparent thermostat 1 is provided with a, the bottom of the transparent thermostat 1 is provided with a plurality of liquid discharge valves 11, the liquid discharge valves 11 are positioned between the bracket 3 and the micro water spray pump 10, four inner side walls of the transparent thermostat 1 are respectively provided with four cleaning nozzles 64, the cleaning nozzles 64 are positioned at the geometric center of the inner side walls of the transparent thermostat 1, the upper part of the inner wall of the transparent thermostat 1 close to one side of the perfusion fixing mechanism 5 is provided with a soft cleaning interface 62, and a cleaning liquid injection hopper 61, the soft cleaning interface 62, a three-way valve III 63, the cleaning nozzles 64, the micro water suction pump 7, the thermostat 8, a liquid guide pipe 9 and the micro water spray pump 10 form a cleaning mechanism 6;
suspension supporting mechanism 4 includes circular chassis box 41, circular chassis box 41 is installed in the middle of transparent thermostated container 1's inner wall bottom, circular chassis box 41 is linked together with miniature water jet pump 10 through water spray interface 12, circular chassis box 41 symmetric connection all around has a plurality of arc stay tubes 42, arc stay tube 42 is connected with transparent thermostated container 1 through stabilizing muscle 45, the one end that circular chassis box 41 was kept away from to arc stay tube 42 is connected with circular stay tube 44, circular chassis box 41, arc stay tube 42 and circular stay tube 44 communicate each other, circular chassis box 41, arc stay tube 42 and circular stay tube 44 inboard all are connected with a plurality of suspension shower nozzle 43, and circular chassis box 41, arc stay tube 42 and circular stay tube 44 all are linked together with suspension shower nozzle 43, the injection direction of suspension shower nozzle 43 that is located circular chassis box 41 faces up, the concentration point of the injection direction of suspension shower nozzle 43 that is located arc stay tube 42 and circular stay tube 44 is located circular stay Above the heart;
the perfusion fixing mechanism 5 comprises an exhaust hole 51, a peristaltic pump 53, a miniature negative pressure device 54 and a support frame 56 from top to bottom, the exhaust hole 51, the peristaltic pump 53, the miniature negative pressure device 54 and the support frame 56 are all arranged on the outer side wall of the transparent constant temperature box 1, the exhaust hole 51 penetrates through the transparent constant temperature box 1 and is connected with an exhaust hose 511, one end of the exhaust hose 511, which is far away from the exhaust hole 51, is connected with an exhaust chamber 512, the peristaltic pump 53 penetrates through the transparent constant temperature box 1 and is connected with a perfusion liquid inlet hose 531, the perfusion liquid inlet hose 531 is communicated with the exhaust chamber 512 through a three-way valve 513, the exhaust chamber 512 is positioned right above the perfusion liquid inlet hose 531, the top end of the perfusion liquid inlet hose 531 is connected with a perfusion liquid inlet joint 532, the liquid inlet end of the miniature negative pressure device 54 penetrates through the transparent constant temperature box 1 and, the one-way valve 542 is close to the perfusion and liquid outlet joint 543, the liquid outlet end of the miniature negative pressure device 54 is connected with a second three-way valve 55, the other two interfaces of the second three-way valve 55 are respectively connected with a blood temporary storage box 561 and a physiological saline waste liquid temporary storage box 562, the blood temporary storage box 561 and the physiological saline waste liquid temporary storage box 562 are placed on the support frame 56, the perfusion and liquid inlet joint 532 is similar to the perfusion and liquid outlet joint 543 in structure, and the device adopts an external power supply for power supply.
In this embodiment: a perfusion fixing system for pathological sections comprises a transparent thermostat 1, wherein transparent fixing liquid is filled in the transparent thermostat 1, the temperature of the fixing liquid is four degrees, the pathological characteristics of kidneys can be kept to the maximum degree, the transparent material is favorable for observing the state of the kidneys, a transparent thermostat cover 2 is rotatably connected to one side of the top end of the transparent thermostat 1, the transparent thermostat cover 2 is convenient to open, a cleaning liquid injection hopper 61, a micro water suction pump 7, a thermostat 8 and a catheter 9 are installed on one side of the outer wall of the short side of the transparent thermostat 1 from top to bottom, the cleaning liquid injection hopper 61 is communicated with the transparent thermostat 1 and the micro water suction pump 7 through a three-way valve three 63, the micro water suction pump 7 is communicated with the thermostat 8, the thermostat 8 is communicated with one end of the catheter 9, the other end of the catheter 9 is communicated with a micro water spray pump 10, and the micro water spray pump 10 is installed in the, the miniature water spray pump 10 is communicated with the suspension supporting mechanism 4 through a water spray interface 12, the miniature water suction pump 7, the thermostat 8, the liquid guide tube 9 and the miniature water spray pump 10 which are installed from top to bottom ensure the operation of the suspension supporting mechanism 4, the thermostat 8 can ensure that the temperature of the fixing liquid in the transparent constant temperature box 1 is kept at four degrees, which is beneficial to the preservation of the isolated kidney, the perfusion fixing mechanism 5 is installed on the other side of the outer wall of the short edge of the transparent constant temperature box 1 and is used for fast perfusion and fixation of the isolated kidney, the support 3 is installed at the bottom of the transparent constant temperature box 1 and is used for supporting the transparent constant temperature box 1, the bottom of the transparent constant temperature box 1 is provided with a plurality of liquid discharge valves 11, the liquid discharge valves 11 are positioned between the support 3 and the miniature water spray pump 10, the bottom of the inner wall of the transparent constant temperature box 1 is sunken towards the liquid, the cleaning nozzle 64 is positioned at the geometric center of the inner side wall of the transparent constant temperature box 1, the used transparent constant temperature box 1 can be thoroughly cleaned, the upper part of the inner wall of the transparent constant temperature box 1 close to one side of the perfusion fixing mechanism 5 is provided with a flexible cleaning interface 62, a used perfusion liquid outlet hose 541 is sleeved on the flexible cleaning interface 62, the perfusion liquid outlet hose 541, a one-way valve 542, a perfusion liquid outlet joint 543, a three-way valve two 55, a blood temporary storage box 561 and a physiological saline waste liquid temporary storage box 562 are cleaned for the next use, and the cleaning liquid injection bucket 61, the flexible cleaning interface 62, the three-way valve three 63, the cleaning nozzle 64, the micro water pump 7, the thermostat 8, the catheter 9 and the micro water spray pump 10 form a cleaning mechanism 6;
suspension supporting mechanism 4 includes circular chassis box 41, circular chassis box 41 is installed in the middle of transparent thermostated container 1's inner wall bottom, circular chassis box 41 is linked together with miniature water jet pump 10 through water spray interface 12, circular chassis box 41 symmetric connection all around has a plurality of arc stay tubes 42, arc stay tube 42 is connected with transparent thermostated container 1 through stabilizing muscle 45, the one end that circular chassis box 41 was kept away from to arc stay tube 42 is connected with circular stay tube 44, circular chassis box 41, arc stay tube 42 and circular stay tube 44 communicate each other, circular chassis box 41, arc stay tube 42 and circular stay tube 44 inboard all are connected with a plurality of suspension shower nozzle 43, and circular chassis box 41, arc stay tube 42 and circular stay tube 44 all are linked together with suspension shower nozzle 43, the injection direction of suspension shower nozzle 43 that is located circular chassis box 41 faces up, the concentration point of the injection direction of suspension shower nozzle 43 that is located arc stay tube 42 and circular stay tube 44 is located circular stay The whole kidney is suspended in the fixing solution above the heart, so that the influence of the outside on the kidney is avoided, and the influence on the pathological characteristics of the kidney is reduced;
the perfusion fixing mechanism 5 comprises an exhaust hole 51, a peristaltic pump 53, a miniature negative pressure device 54 and a support frame 56 from top to bottom, the exhaust hole 51, the peristaltic pump 53, the miniature negative pressure device 54 and the support frame 56 are all arranged on the outer side wall of the transparent constant temperature box 1, the exhaust hole 51 penetrates through the transparent constant temperature box 1 to be connected with an exhaust hose 511, one end of the exhaust hose 511, which is far away from the exhaust hole 51, is connected with an exhaust chamber 512, when in use, the exhaust chamber 512 is used for exhausting air filled in the kidney to avoid perfusion fixing failure, the peristaltic pump 53 penetrates through the transparent constant temperature box 1 to be connected with a perfusion liquid inlet hose 531, the perfusion liquid inlet hose 531 is communicated with the exhaust chamber 512 through a three-way valve I513, the exhaust chamber 512 is positioned right above the perfusion liquid inlet hose 531, the top end of the perfusion liquid inlet hose 531 is connected with a perfusion liquid inlet joint 532 used for inputting physiological, the top end of the perfusion liquid outlet hose 541 is connected with a perfusion liquid outlet joint 543, the inner wall of the perfusion liquid outlet hose 541 is connected with a one-way valve 542, the one-way valve 542 is close to the perfusion liquid outlet joint 543 and is used for outputting blood and physiological saline, the one-way valve 542 can prevent the liquid from flowing back to the kidney, the liquid outlet end of the miniature negative pressure device 54 is connected with a two-way valve 55, the other two interfaces of the two-way valve 55 are respectively connected with a blood temporary storage box 561 and a physiological saline waste liquid temporary storage box 562, the perfused waste liquid is stored respectively, the subsequent treatment of the waste liquid is convenient, the blood temporary storage box 561 and the physiological saline waste liquid temporary storage box 562 are placed on the support frame 56, and the perfusion liquid inlet joint 532 is similar to the perfusion liquid outlet joint structure, so that the whole isolated kidney can be fixed quickly, the pathological characteristics of the isolated kidney are.
Specifically, the outer wall of the perfusion liquid inlet joint 532 is connected with two groups of anti-slip protrusions 5321, the perfusion liquid inlet joint 532 is sleeved with an elastic fixing ring 5322, the elastic fixing ring 5322 is positioned between the anti-slip protrusions 5321, the inner diameter of the elastic fixing ring 5322 is equal to the outer diameter of the soft cleaning interface 62, and the top end of the perfusion liquid inlet joint 532 is of a conical structure; the perfusion liquid inlet joint 532 can be connected with the artery vessel of the kidney more firmly, and the perfusion liquid inlet joint 532 is prevented from being separated from the artery vessel of the kidney, so that the perfusion failure is avoided.
Specifically, the suspension spray head 43 comprises a suspension spray head body 431 and a suspension spray head liquid storage chamber 432, the suspension spray head liquid storage chamber 432 is of a conical structure, the funnel end of the suspension spray head 43 is communicated with the suspension spray head body 431, and the conical bottom end of the suspension spray head liquid storage chamber 432 is provided with a plurality of suspension spray head spray holes 433 which are regularly distributed; the liquid sprayed by the suspension nozzle 43 can support the kidney without affecting the pathological features of the kidney.
Specifically, a drying fan 21 is installed in the middle of the transparent thermostatic box cover 2, exhaust fans 22 are installed on two sides of the middle of the transparent thermostatic box cover 2, the drying fan 21 and the exhaust fans 22 penetrate through the transparent thermostatic box cover 2, one side of the transparent thermostatic box cover 2 is rotatably connected with the transparent thermostatic box 1, and the other side of the transparent thermostatic box cover 2 is connected with a handle 23; the cleaned transparent thermostatic case cover 2 can be dried, and the use for the next time is convenient.
Specifically, the vent hole 51 is a circular elastic piece, the inner wall of the vent hole 51 is connected with a syringe 52 in an interference manner, and the diameter of the nipple of the syringe 52 is larger than the inner diameter of the vent hole 51; the tightness of the exhaust hole 51, the exhaust hose 511 and the exhaust chamber 512 is ensured, and the air filled in the kidney is conveniently exhausted.
Specifically, the micro water spray pump 10 is connected with the soft cleaning interface 62 and the cleaning nozzle 64 through a valve 65; when the whole device operates, the valve 65 is closed, the cleaning mechanism 6 is prevented from influencing the operation of the perfusion fixing mechanism 5, and when the device is cleaned, the valve 65 is opened to clean the whole device.
Specifically, the top end of the cleaning nozzle 64 is conical, and the top end and the side surface of the conical shape are provided with a plurality of nozzles; the cleaning spray head 64 can be cleaned more thoroughly for the entire apparatus.
The working principle and the using process of the invention are as follows: preparing enough physiological saline and 4% paraformaldehyde solution (ice box precooling), arranging a peristaltic pump 53, adjusting the flow rate, slowly and stably opening a transparent constant temperature box cover 2, filling four-degree fixing liquid into a transparent constant temperature box 1, opening a suspension supporting mechanism 4, putting the kidney to be perfused and fixed, pumping the physiological saline into a perfusion liquid inlet hose 531, inserting a perfusion liquid inlet joint 532 into a renal artery blood vessel, fixing the kidney artery blood vessel by using an elastic fixing ring 5322, inserting an injector 52, sucking out gas in an exhaust hose 511 and an exhaust chamber 512, sucking air filled into the kidney into the exhaust chamber 512 by pouring air into the exhaust chamber 512, adjusting a three-way valve I513, closing the exhaust chamber 512, communicating the perfusion liquid inlet hose 531 with the perfusion liquid inlet joint 532, connecting a perfusion liquid outlet joint 543 with a renal vein blood vessel, opening a perfusion fixing mechanism 5, pumping the physiological saline into the kidney by the peristaltic pump 53 through the perfusion liquid inlet hose 531 and the perfusion liquid inlet joint 532, conveying normal saline to each part of the kidney through the systemic circulation of the kidney, pumping the normal saline into the kidney to clean the blood in the kidney, injecting the blood flowing out into the blood temporary storage box 561 through the perfusion fluid hose 541 under the action of the miniature negative pressure device 54, observing the perfusion fluid hose 541, recording the used time, changing 4% paraformaldehyde solution when the blood is discharged completely, starting to fix the kidney, adjusting the two-way valve 55 to close the passage of the blood temporary storage box 561, opening the passage of the normal saline waste fluid temporary storage box 562, contrasting the recorded time, observing the color of the kidney, completing the perfusion fixation of the kidney when the color of the kidney is whitened from the time recorded last time, closing the perfusion fixation mechanism 5, taking out the kidney after soaking for at least two hours, thereby achieving better fixation effect, after the device is used, the fixing liquid in the transparent constant temperature box 1 is discharged through the liquid discharge valve 11, after the fixing liquid in the transparent constant temperature box 1 is discharged, the peristaltic pump 53 is started, the cleaning liquid is injected into the peristaltic pump 53, the cleaning peristaltic pump 53, the filling liquid inlet hose 531 and the filling liquid inlet connector 532 are cleaned, the filling liquid outlet connector 543 is connected to the soft cleaning connector 62, the transparent constant temperature box cover 2 is covered, the suspension support mechanism 4 and the cleaning mechanism 6 are opened, the three-way valve three 63 is adjusted, the passage of the cleaning liquid injection hopper 61 and the transparent constant temperature box 1 is closed, the passage of the cleaning liquid injection hopper 61 and the micro water pump 7 is opened, the cleaning liquid is injected into the cleaning liquid injection hopper 61, the whole device is cleaned, after the cleaning of the whole device is completed, the suspension support mechanism 4 and the cleaning mechanism 6 are closed, and the drying fan 21 and.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A perfusion fixation system for pathological sections, comprising a transparent incubator (1), characterized in that: transparent thermostated container (1) top one side is rotated and is connected with transparent thermostated container lid (2), transparent thermostated container (1) minor face outer wall one side top-down installs washing liquid injection fill (61), miniature suction pump (7), thermostat (8) and catheter (9), washing liquid injection fill (61) through three-way valve three (63) with transparent thermostated container (1) with miniature suction pump (7) are linked together, miniature suction pump (7) with thermostat (8) are linked together, thermostat (8) with the one end of catheter (9) is linked together, the other end and miniature spray pump (10) of catheter (9) are linked together, install in miniature spray pump (10) the bottom central authorities of transparent thermostated container (1), miniature spray pump (10) are linked together through water spray interface (12) and suspension supporting mechanism (4), transparent thermostated container (1) minor face outer wall opposite side is installed and is filled fixed establishment (5), support (3) are installed to transparent thermostated container (1) bottom, a plurality of flowing back valve (11) have been seted up to transparent thermostated container (1) bottom, flowing back valve (11) are located support (3) with between miniature water jet pump (10), four washing shower nozzle (64) are installed respectively to four inside walls of transparent thermostated container (1), just wash shower nozzle (64) and be located the geometric centre of transparent thermostated container (1) inside wall, transparent thermostated container (1) are close to fill inner wall upper portion of fixed establishment (5) one side and install soft washing interface (62), washing liquid injection fill (61) soft washing interface (62) three-way valve (63) wash shower nozzle (64) miniature water jet pump (7), The thermostat (8), the catheter (9) and the micro water spray pump (10) form a cleaning mechanism (6);
the suspension supporting mechanism (4) comprises a circular bottom plate box (41), the circular bottom plate box (41) is installed in the middle of the bottom of the inner wall of the transparent thermostat (1), the circular bottom plate box (41) is communicated with the micro water spray pump (10) through the water spray interface (12), a plurality of arc supporting tubes (42) are symmetrically connected to the periphery of the circular bottom plate box (41), the arc supporting tubes (42) are connected with the transparent thermostat (1) through stabilizing ribs (45), one ends, far away from the circular bottom plate box (41), of the arc supporting tubes (42) are connected with circular supporting tubes (44), the circular bottom plate box (41), the arc supporting tubes (42) and the circular supporting tubes (44) are communicated with each other, the circular bottom plate box (41), the arc supporting tubes (42) and the inner side of the circular supporting tubes (44) are connected with a plurality of suspension nozzles (43), the circular base plate box (41), the arc-shaped supporting pipe (42) and the circular supporting pipe (44) are communicated with the suspension spray head (43), the spraying direction of the suspension spray head (43) on the circular base plate box (41) is upward, and a concentration point of the spraying directions of the suspension spray head (43) on the arc-shaped supporting pipe (42) and the circular supporting pipe (44) is positioned above the center of the circle of the circular supporting pipe (44);
fill fixed establishment (5) and include exhaust hole (51), peristaltic pump (53), miniature negative pressure ware (54) and support frame (56) of top-down, exhaust hole (51), peristaltic pump (53), miniature negative pressure ware (54) and support frame (56) are all installed on the lateral wall of transparent thermostated container (1), exhaust hole (51) are passed transparent thermostated container (1) is connected with exhaust hose (511), exhaust hose (511) are kept away from the one end of exhaust hole (51) is connected with exhaust chamber (512), peristaltic pump (53) are passed transparent thermostated container (1) are connected with fills inlet fluid hose (531), fill inlet fluid hose (531) through three-way valve one (513) with exhaust chamber (512) are linked together, exhaust chamber (512) are located directly over filling inlet fluid hose (531), the top of filling inlet fluid hose (531) is connected with fills inlet fluid connector (532), the liquid inlet end of the miniature negative pressure device (54) penetrates through the transparent thermostat (1) and is connected with a perfusion liquid outlet hose (541), the top end of the perfusion liquid outlet hose (541) is connected with a perfusion liquid outlet joint (543), the inner wall of the perfusion liquid outlet hose (541) is connected with a one-way valve (542), the one-way valve (542) is close to the perfusion liquid outlet joint (543), the liquid outlet end of the miniature negative pressure device (54) is connected with a three-way valve II (55), the other two interfaces of the three-way valve II (55) are respectively connected with a blood temporary storage box (561) and a physiological saline waste liquid temporary storage box (562), the blood temporary storage box (561) and the physiological saline waste liquid temporary storage box (562) are placed on the support frame (56), and the perfusion liquid inlet joint (532) and the perfusion liquid outlet joint (543) are structurally similar.
2. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the outer wall of filling liquid inlet joint (532) is connected with two sets of anti-skidding protrudingly (5321), fill liquid inlet joint (532) suit elastic fixation circle (5322), just elastic fixation circle (5322) are located between anti-skidding protrudingly (5321), the internal diameter of elastic fixation circle (5322) equals the external diameter of soft washing interface (62), the top of filling liquid inlet joint (532) is the toper structure.
3. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the suspension spray head (43) comprises a suspension spray head body (431) and a suspension spray head liquid storage chamber (432), the suspension spray head liquid storage chamber (432) is of a conical structure, the funnel end of the suspension spray head (43) is communicated with the suspension spray head body (431), and a plurality of suspension spray head spray holes (433) which are regularly distributed are formed in the conical bottom end of the suspension spray head liquid storage chamber (432).
4. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the mid-mounting of transparent constant temperature case lid (2) has drying fan (21), exhaust fan (22) are installed to the middle part both sides of transparent constant temperature case lid (2), drying fan (21) with exhaust fan (22) run through transparent constant temperature case lid (2), one side of transparent constant temperature case lid (2) with transparent thermostated container (1) rotates and connects, the opposite side of transparent constant temperature case lid (2) is connected with handle (23).
5. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the exhaust hole (51) is a circular ring-shaped elastic piece, the inner wall of the exhaust hole (51) is connected with a syringe (52) in an interference mode, and the diameter of a nipple of the syringe (52) is larger than the inner diameter of the exhaust hole (51).
6. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the micro water spray pump (10) is connected with the soft cleaning interface (62) and the cleaning spray head (64) through a valve (65).
7. A perfusion fixation system for pathological sections according to claim 1, characterized in that: the top end of the cleaning spray head (64) is conical, and a plurality of nozzles are arranged at the top end and the side surface of the conical shape.
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