CN111134110A - In vitro kidney perfusion system - Google Patents

In vitro kidney perfusion system Download PDF

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Publication number
CN111134110A
CN111134110A CN201911404577.8A CN201911404577A CN111134110A CN 111134110 A CN111134110 A CN 111134110A CN 201911404577 A CN201911404577 A CN 201911404577A CN 111134110 A CN111134110 A CN 111134110A
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China
Prior art keywords
pipe
liquid
outflow
perfusion system
outlet
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CN201911404577.8A
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Chinese (zh)
Inventor
田向永
曹广辉
吴轩
段文静
武小强
王志为
李玉荣
闫天中
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Henan Provincial Peoples Hospital
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Henan Provincial Peoples Hospital
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Priority to CN201911404577.8A priority Critical patent/CN111134110A/en
Publication of CN111134110A publication Critical patent/CN111134110A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0247Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • External Artificial Organs (AREA)

Abstract

The invention discloses an in vitro kidney perfusion system, which relates to the technical field of kidney transplantation, and is characterized in that a liquid conveying pipeline is taken as a main body, a liquid inlet pipe, a liquid dropping funnel, a fluid speed regulation roller and an outflow assembly are sequentially and serially connected on the liquid conveying pipeline, and through the arrangement of the structure, the defect that a perfusion device used in the kidney transplantation process in the prior art is usually a self-made simple device can be overcome, the simple device is inconvenient when multiple blood vessels or the blood vessels are thin, when the kidney is perfused, medicine can be injected through the device, the flow rate can be regulated through a pulley, pressure can be generated during liquid conversion and extrusion through a soft ball, perfusion is convenient, a proper silicone tube can be selected according to the thickness and the number of renal arteries for perfusion, and the perfusion system is simple in structure, wide in material, convenient to operate, practical, low in price and low in cost, and has the advantages, The advantage of fully pouring, scientific and reasonable structure, safe and convenient use.

Description

In vitro kidney perfusion system
Technical Field
The invention relates to the technical field of kidney transplantation, in particular to an in vitro kidney perfusion system.
Background
Kidney transplant surgery generally takes about three to four hours. The operation is performed by abdominal incision, with the implanted new kidney placed in the lower abdomen near the groin (e.g., right iliac fossa), without removing the original two kidneys, by merely connecting the blood vessel of the new kidney to the blood vessel of the donor's abdomen (e.g., internal/external iliac artery, external iliac vein) and implanting the ureter of the new kidney into the bladder wall of the donor.
In the kidney transplantation operation, when the kidney is required to be perfused with a 4 ℃ preservation solution immediately after being isolated from the body, the blood in the kidney is flushed out in time until the color of the kidney is pale and no red liquid flows out.
For the above process, the adopted tools, chinese patent, disclose the following patent documents:
the patent content of the perfusion tube for the kidney with the publication number of CN206333260U comprises a tube body, wherein an air bag and a water outlet hole are sequentially arranged on the tube body from the head to the tail of the tube body; the auxiliary pipe body is sleeved in the pipe body and is connected with the pipe body in a sliding manner; the auxiliary pipe body comprises a first air pipe and a water inlet hole which are sequentially arranged and communicated with the air bag; the auxiliary pipe body is closed with the water outlet hole in a natural state, and when the auxiliary pipe body is filled with water, the auxiliary pipe body axially moves to open the water outlet hole and change the size of the water outlet hole. The utility model realizes the control of the perfusion speed through the sliding auxiliary pipe body; the blood vessel sealing device is convenient for sealing blood vessels, simple in structure, convenient to operate, strong in sealing performance and free of damage to organisms; removing air bubbles from the perfusate to prevent the air bubbles from entering the kidney; the kidney perfusion tube is prevented from being placed too deeply, mechanical damage is caused to the renal artery intima, and the kidney function is prevented from being influenced; reduce the incidence of vascular complications after kidney transplantation, thereby improving the possibility of patient recovery.
Notice is CN 203647837U's separation kidney perfusion tube, and this patent technical content includes the connector tube head, sets up annular arch of anti-overflow on the connector tube head and grafting pipeline, the annular arch of anti-overflow goes up the side and is level and smooth arcwall face, the annular protruding downside of anti-overflow is the arcwall face, grafting pipeline intercommunication the connector tube head. After the in-vitro kidney perfusion tube is inserted into the renal artery with the corresponding inner diameter, the connector head and the anti-overflow annular bulge enter the renal artery, and the anti-overflow annular bulge is used for lightly supporting the renal artery, so that the sealing performance of the joint is good, and perfusion liquid is prevented from flowing back during perfusion; the smooth connector head and the smooth anti-overflow annular bulge on the connector head avoid mechanical damage to the intima of the renal artery; has the advantages of scientific design, reasonable structure, simple operation, prevention of the overflow leakage of the perfusion liquid and avoidance of the damage of the endarterium caused by the mechanicalness.
The perfusion devices used during the transplantation of the kidney, which are referred to in the above patent contents and in the prior art, are generally self-made simple devices, which have many inconveniences when multiple vessels are encountered or when the vessels are thin, and for this reason, the skilled person has proposed an ex vivo kidney perfusion system.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an in vitro kidney perfusion system, which solves the problem that a perfusion device used in the kidney transplantation process in the prior art is a self-made simple device, and the simple device is inconvenient when multiple blood vessels or thin blood vessels are encountered.
In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides an in vitro kidney perfusion system, includes the conveying house steward, the fixed feed liquor pipe that concatenates of inlet at conveying house steward top is provided with, the both sides of feed liquor socle portion district section and with the adjacent position of conveying house steward and feed liquor pipe tie point are fixed respectively to concatenate and are provided with medicine injection pipe and air delivery pipe, the one end that medicine injection pipe and air delivery pipe outwards extended corresponds fixedly connected with nut and air cleaner respectively, the top district section of conveying house steward is provided with the liquid drip chamber with the fixed concatenation in the adjacent position of feed liquor pipe, the fixed concatenation of liquid outlet of conveying house steward is provided with the subassembly that outflows, the bottom district section of conveying house steward just cup joints with the outside activity at the adjacent position of.
The liquid dropping funnel comprises a funnel body fixedly connected with the conveying main pipe section in series, the inner top end of the funnel body is fixedly connected with a filter screen supporting frame, and the inner portion of the filter screen supporting frame is fixedly connected with a filter screen.
The subassembly that flows out is including fixed and the soft ball that concatenates mutually with conveying house steward liquid outlet, the liquid outlet end of soft ball is fixed to concatenate the feed liquor end that is provided with the four-way pipe, the three play liquid end of four-way pipe corresponds to concatenating respectively and is provided with first outlet pipe, second outlet pipe and third outlet pipe, first outlet pipe, second outlet pipe and third outlet pipe are three materials the same, the different component of diameter size.
Preferably, the top of the liquid inlet pipe is provided with a total inlet of liquid, the total inlet is in a conical head shape, and the bottom section of the liquid inlet pipe is fixedly sleeved with a handle at a position above the connection point of the liquid inlet pipe and the medicine injection pipe.
Preferably, the outer part of the middle section of the medicine injection tube is connected with a first non-return clamp in a clamping mode, and the inner wall of the screw cap is provided with a thread groove matched with the injector head.
Preferably, the liquid inlet pipe, the delivery main pipe, the drug injection pipe, the air delivery pipe, the liquid dropping funnel and the outflow assembly form an integrated liquid delivery structure together.
Preferably, the soft ball is of an ellipsoidal structure made of plastic.
Preferably, first outflow pipe, second outflow pipe and third outflow pipe are the silica gel material, and the diameter size of first outflow pipe is 1mm, the diameter size of second outflow pipe is 2mm, the diameter size of third outflow pipe is less than 1 mm.
Preferably, two channels are arranged inside the liquid inlet pipe, one channel is connected with the medicine injection pipe in series, and the other channel is connected with the conveying main pipe and the air conveying pipe in series.
Preferably, the outer section of first outlet pipe, second outlet pipe and third outlet pipe and all correspond to be provided with a lug, three apart from the position of liquid outlet 2cm the shape of lug all is wing form, the outer section of first outlet pipe, second outlet pipe and third outlet pipe all corresponds respectively through the centre gripping mode and connects a second and end contrary clamp.
Advantageous effects
The invention provides an in vitro kidney perfusion system. Compared with the prior art, the method has the following beneficial effects:
1. this separation kidney system of infusing, be the main part with the pipeline of a liquid, it has the feed liquor pipe to concatenate in proper order on this pipeline, the liquid drip chamber, fluid speed governing gyro wheel and the subassembly that outflows, setting through above-mentioned structure, can compensate among the prior art at the used perfusion device of kidney transplantation in-process, be the simple and easy device of self-control usually, this kind of simple and easy device has the defect of a lot of inconveniences when meetting many blood vessels or blood vessel when thinner, when pouring into the kidney, can annotate the medicine through aforementioned device, can adjust the velocity of flow through the pulley, generate pressure when can realizing liquid conversion and extrusion through the soft ball, conveniently pour into, can select suitable silicone tube to pour into according to renal artery's thickness and quantity, simple structure, the material is extensive, convenient operation, low-priced practicality, fill abundant advantage.
2. This separation kidney perfusion system, liquid drip chamber include the fixed drip chamber body of concatenating on the main district's section of carrying, the inside top fixedly connected with filter screen braced frame of drip chamber body, filter screen braced frame's inside fixedly connected with filter screen is provided with the filter screen in the inside of liquid drip chamber, can carry out filtration treatment to the liquid that is about to fill to transplanting the inside of kidney, improves the protection to transplanting the back kidney, structure scientific and reasonable, convenience safe in utilization.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the liquid dropping funnel of the present invention;
FIG. 3 is a schematic diagram of the outflow assembly of the present invention.
In the figure: 1. a delivery main pipe; 2. a liquid inlet pipe; 3. a conical head; 4. a handle; 5. a drug injection tube; 6. a first check clip; 7. a nut; 8. an air delivery pipe; 9. an air filter; 10. a liquid dropping funnel; 101. a drip chamber body; 102. a screen support frame; 103. filtering with a screen; 11. a fluid speed regulation roller; 12. an outflow assembly; 121. a soft ball; 122. a four-way pipe; 123. a first outlet pipe; 124. a second outlet pipe; 125. a third outlet pipe; 126. a second backstop clamp.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution: an in-vitro kidney perfusion system comprises a conveying main pipe 1, a liquid inlet at the top of the conveying main pipe 1 is fixedly connected in series with a liquid inlet pipe 2, the top of the liquid inlet pipe 2 is set as a total inlet of liquid, the shape of the total inlet is set as a conical head 3, a handle 4 is fixedly sleeved and connected at the bottom section of the liquid inlet pipe 2 and at the position above the connection point of the liquid inlet pipe 2 and a medicine injection pipe 5, the two sides of the bottom section of the liquid inlet pipe 2 and the position adjacent to the connection point of the conveying main pipe 1 and the liquid inlet pipe 2 are respectively fixedly connected in series with the medicine injection pipe 5 and an air conveying pipe 8, one end of the medicine injection pipe 5 extending outwards and one end of the air conveying pipe 8 extend outwards are respectively and correspondingly and fixedly connected with a screw cap 7 and an air filter 9, the outside of the middle section of the medicine injection pipe 5 is connected with, the top section of carrying house steward 1 and the fixed liquid drip chamber 10 that concatenates in the adjacent position of feed liquor pipe 2, the fixed liquid outlet that concatenates of carrying house steward 1 is provided with outflow assembly 12, the bottom section of carrying house steward 1 and cup joint with the outside activity that outflows the adjacent position of assembly 12 and be provided with fluid speed governing gyro wheel 11, feed liquor pipe 2, carrying house steward 1, medicine injection pipe 5, air transport pipe 8, liquid drip chamber 10 and the liquid transport structure who outflows assembly 12 and form the integral type jointly, the inside of feed liquor pipe 2 is provided with two passageways, a passageway concatenates with medicine injection pipe 5 mutually, another passageway concatenates mutually with carrying house steward 1 and air transport pipe 8.
Referring to fig. 2, the liquid dropping funnel 10 includes a funnel body 101 fixedly connected in series to a section of the conveying main pipe 1, a filter screen supporting frame 102 is fixedly connected to an inner top end of the funnel body 101, and a filter screen 103 is fixedly connected to an inner portion of the filter screen supporting frame 102.
Referring to fig. 3, the outflow assembly 12 includes a soft ball 121 fixed to the liquid outlet of the conveying main pipe 1 and connected in series, the soft ball 121 is an ellipsoidal structure made of plastic, the liquid outlet of the soft ball 121 is fixed to the liquid inlet of the four-way pipe 122 connected in series, three liquid outlets of the four-way pipe 122 are respectively and correspondingly connected in series with a first outflow pipe 123, a second outflow pipe 124 and a third outflow pipe 125, the first outflow pipe 123, the second outflow pipe 124 and the third outflow pipe 125 are three members with the same material and different diameters, the first outflow pipe 123, the second outflow pipe 124 and the third outflow pipe 125 are made of silica gel, the diameter of the first outflow pipe 123 is 1mm, the diameter of the second outflow pipe 124 is 2mm, the diameter of the third outflow pipe 125 is less than 1mm, the outer sections of the first outflow pipe 123, the second outflow pipe 124 and the third outflow pipe 125 are provided with a bump corresponding to the position 2cm away from the liquid outlet, the three bumps are wing-shaped, and the external sections of the first outlet pipe 123, the second outlet pipe 124 and the third outlet pipe 125 are respectively and correspondingly connected with a second non-return clamp 126 in a clamping manner.
When in use, before the perfusion treatment, the perfusion system needs to be assembled according to the state shown in fig. 1, after the assembly, the liquid inlet pipe 2 is inserted into a container storing perfusion liquid, when corresponding medicine needs to be added into the perfusion liquid, the medicine injection pipe 5 can be connected with an injector which is externally provided with the medicine by the screw cap 7, the first check clip 6 is taken down, the medicine can be injected into the perfusion liquid, then the perfusion liquid flows along the conveying main pipe 1 and passes through the drip hopper body 101, the filter screen 103 is arranged inside the drip hopper body 101, the perfusion liquid can be filtered, the filtered perfusion liquid enters the soft ball 121 by the conveying main pipe 1 and enters the interior of the four-way pipe 122, then, the corresponding flow outlet pipe can be opened according to the actual requirement, and the perfusion device used in the kidney transplantation process in the prior art can be compensated by the arrangement of the structure, usually for self-made simple and easy device, this kind of simple and easy device has the defect of a lot of inconveniences when meetting many blood vessels or blood vessel is thinner, when filling the kidney, can annotate the medicine through aforementioned device, can adjust the velocity of flow through the pulley, generate pressure when can realizing liquid conversion and extrusion through soft ball 121, conveniently fill, can fill according to the suitable play flow tube of renal artery thickness and quantity selection, at simple structure, the material is extensive, convenient operation, low-priced practicality, fill abundant advantage.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (8)

1. An ex vivo renal perfusion system comprising a delivery manifold (1), characterized in that: a liquid inlet pipe (2) is fixedly connected in series with a liquid inlet at the top of the conveying main pipe (1), the two sides of the bottom section of the liquid inlet pipe (2) and the parts adjacent to the connection point of the conveying main pipe (1) and the liquid inlet pipe (2) are respectively fixedly connected in series with a medicine injection pipe (5) and an air conveying pipe (8), one end of the medicine injection tube (5) and one end of the air delivery tube (8) which extend outwards are respectively and fixedly connected with a screw cap (7) and an air filter (9) correspondingly, the top section of the conveying main pipe (1) is fixedly connected with the part adjacent to the liquid inlet pipe (2) in series with a liquid dropping funnel (10), the liquid outlet of the conveying main pipe (1) is fixedly connected in series with an outflow component (12), a fluid speed regulation roller (11) is movably sleeved on the outer side of the bottom section of the conveying main pipe (1) and the part adjacent to the outflow component (12);
the liquid dropping funnel (10) comprises a funnel body (101) which is fixedly connected with the section of the conveying main pipe (1) in series, the top end inside the funnel body (101) is fixedly connected with a filter screen supporting frame (102), and the inside of the filter screen supporting frame (102) is fixedly connected with a filter screen (103);
outflow assembly (12) are including fixed soft ball (121) that concatenate mutually with conveying house steward (1) liquid outlet, the liquid outlet end of soft ball (121) is fixed to concatenate the feed liquor end that is provided with four-way pipe (122), the three play liquid end of four-way pipe (122) corresponds respectively to concatenate and is provided with first outflow pipe (123), second outflow pipe (124) and third outflow pipe (125), first outflow pipe (123), second outflow pipe (124) and third outflow pipe (125) are three materials the same, the different component of diameter size.
2. The ex vivo kidney perfusion system of claim 1, wherein: the top of feed liquor pipe (2) sets up to the total import of liquid, and the shape of this total import sets up to conical head (3), the bottom section of feed liquor pipe (2) just is located the fixed cup joint in position department above feed liquor pipe (2) and medicine injection pipe (5) tie point and is provided with handle (4).
3. The ex vivo kidney perfusion system of claim 1, wherein: the outer part of the middle section of the medicine injection tube (5) is connected with a first non-return clamp (6) in a clamping mode, and the inner wall of the screw cap (7) is provided with a thread groove matched with the injector head.
4. The ex vivo kidney perfusion system of claim 1, wherein: the liquid inlet pipe (2), the conveying main pipe (1), the medicine injection pipe (5), the air conveying pipe (8), the liquid dropping funnel (10) and the outflow assembly (12) form an integrated liquid conveying structure together.
5. The ex vivo kidney perfusion system of claim 1, wherein: the soft ball (121) is of an ellipsoidal structure made of plastic.
6. The ex vivo kidney perfusion system of claim 1, wherein: first play flow tube (123), second play flow tube (124) and third play flow tube (125) are the silica gel material, and the diameter size of first play flow tube (123) is 1mm, the diameter size of second play flow tube (124) is 2mm, the diameter size of third play flow tube (125) is less than 1 mm.
7. The ex vivo kidney perfusion system of claim 1, wherein: two channels are arranged in the liquid inlet pipe (2), one channel is connected with the medicine injection pipe (5) in series, and the other channel is connected with the conveying main pipe (1) and the air conveying pipe (8) in series.
8. The ex vivo kidney perfusion system of claim 1, wherein: the outer sections of the first outlet pipe (123), the second outlet pipe (124) and the third outlet pipe (125) are correspondingly provided with a lug at a position 2cm away from the liquid outlet, the three lugs are wing-shaped, and the outer sections of the first outlet pipe (123), the second outlet pipe (124) and the third outlet pipe (125) are correspondingly connected with a second non-return clamp (126) respectively in a clamping mode.
CN201911404577.8A 2019-12-31 2019-12-31 In vitro kidney perfusion system Pending CN111134110A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112616830A (en) * 2020-12-29 2021-04-09 亳州市人民医院 Perfusion fixing system for pathological section

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Publication number Priority date Publication date Assignee Title
CN2278491Y (en) * 1997-01-16 1998-04-15 河南省人民医院 Disposable conjunctival sac perfusion device
CN201481888U (en) * 2009-09-15 2010-05-26 刘平 Perfusion apparatus special for urological department intracavity detritus
CN104039135A (en) * 2011-11-10 2014-09-10 奥加诺克斯有限责任公司 Organ perfusion systems
CN206252701U (en) * 2016-08-22 2017-06-16 中国医科大学附属盛京医院 Multiplex washing pipe light proofing infusion apparatus
CN206372323U (en) * 2016-08-31 2017-08-04 威海威创医疗器械有限公司 A kind of Continuous Perfusion drainage system
CN206930504U (en) * 2017-07-03 2018-01-26 厦门大学附属第一医院 A kind of device for casting for being used to remove myocardium vessel endothelium
CN207653422U (en) * 2017-12-21 2018-07-27 杭州莱普晟医疗科技有限公司 A kind of liver perfusion pipe-line system
CN109199527A (en) * 2017-06-30 2019-01-15 微创心脉医疗科技(上海)有限公司 Mechanical thrombus remove device

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2278491Y (en) * 1997-01-16 1998-04-15 河南省人民医院 Disposable conjunctival sac perfusion device
CN201481888U (en) * 2009-09-15 2010-05-26 刘平 Perfusion apparatus special for urological department intracavity detritus
CN104039135A (en) * 2011-11-10 2014-09-10 奥加诺克斯有限责任公司 Organ perfusion systems
CN206252701U (en) * 2016-08-22 2017-06-16 中国医科大学附属盛京医院 Multiplex washing pipe light proofing infusion apparatus
CN206372323U (en) * 2016-08-31 2017-08-04 威海威创医疗器械有限公司 A kind of Continuous Perfusion drainage system
CN109199527A (en) * 2017-06-30 2019-01-15 微创心脉医疗科技(上海)有限公司 Mechanical thrombus remove device
CN206930504U (en) * 2017-07-03 2018-01-26 厦门大学附属第一医院 A kind of device for casting for being used to remove myocardium vessel endothelium
CN207653422U (en) * 2017-12-21 2018-07-27 杭州莱普晟医疗科技有限公司 A kind of liver perfusion pipe-line system

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112616830A (en) * 2020-12-29 2021-04-09 亳州市人民医院 Perfusion fixing system for pathological section

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Application publication date: 20200512