CN115979749A - Perfusion fixing device for pathological section - Google Patents
Perfusion fixing device for pathological section Download PDFInfo
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- CN115979749A CN115979749A CN202211554523.1A CN202211554523A CN115979749A CN 115979749 A CN115979749 A CN 115979749A CN 202211554523 A CN202211554523 A CN 202211554523A CN 115979749 A CN115979749 A CN 115979749A
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Abstract
The invention discloses a perfusion fixing device for pathological sections, and relates to the technical field of perfusion fixing; the fixing base is connected with external equipment, the fixing frame is installed above the fixing base, a plurality of fastening supports are installed on the side face of the fixing frame, liquid storage pipes of injector structures are installed in the fastening supports in a plugging mode, and a plurality of guide grooves are further formed in the side face of the fixing frame. According to the invention, the flow velocity and the pressure of the perfusion solution flowing out of the liquid injection mechanism are close to the body fluid flow of an experimental animal, the integrity of the tissue sample is protected to the maximum extent, the damage to the internal structure and cells of the tissue sample is avoided, the compression on the internal and external surfaces of the tissue is avoided in the tissue sample acquisition process, the perfusion flow and the pressure are controlled in a targeted manner according to the size and the type of the tissue sample, the pathological characteristic fixing effect of the tissue sample is effectively improved, the perfusion process is convenient to control, and the perfusion fixing operation is simplified.
Description
Technical Field
The invention relates to the technical field of perfusion fixing, in particular to a perfusion fixing device for pathological sections.
Background
Pathological section is prepared by taking pathological tissues with a certain size and making pathological section by a histopathology method (usually, the pathological tissues are embedded in a paraffin block and cut into slices by a slicer, and then hematoxylin-eosin (H-E) staining is carried out), and the pathological changes and the occurrence and development processes of the pathological changes are further examined by a microscope, and finally, an examination means for pathological diagnosis is made.
Generally speaking, tissue samples of pathological sections can be subjected to corresponding histochemical staining only after the steps of material taking, fixing, embedding, slicing and the like, in order to better keep the inherent morphological structure, chemical component distribution, reactivity and the like of tissues and cells, the tissue samples are fixed in the sampling process, a perfusion fixing method is the most commonly adopted pathological characteristic fixing method in the tissue sample sampling process, physiological saline is firstly injected into blood vessels of the tissue samples in the perfusion fixing process, the blood is discharged from the whole tissue samples or the whole body through blood vessel branches, then a perfusion solid reagent is injected into the blood vessels, and the blood reaches the whole tissue or the whole body through the blood vessel branches, so that the pathological characteristics of the whole tissue or organ are fully fixed, the conventional physiological saline and perfusion solid reagents mainly adopt devices such as a hanging bottle or an injector to drain and replace the blood, or a plurality of persons are matched with each other to operate, the tissue samples are mostly clamped by tools such as a clamp and the like in the tissue sample separation process, the external of the tissue samples can be pressed, the operation time of solution replacement, the perfusion speed control and the internal operation of the tissue samples can not influence the pathological characteristic analysis of the tissue samples.
In view of the above, the present invention provides a perfusion fixing device for pathological sections, so as to solve the technical problems in the prior art.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides a perfusion fixing device for pathological sections.
The invention provides a perfusion fixing device for pathological sections, which comprises a fixed seat connected with external equipment, wherein a fixed frame is arranged above the fixed seat, a plurality of fastening brackets are arranged on the side surface of the fixed frame, a liquid storage pipe with an injector structure is arranged in each fastening bracket in a plugging manner, a plurality of guide grooves are also arranged on the side surface of the fixed frame, hydraulic adjusting mechanisms are arranged in the guide grooves in a sliding manner, and the hydraulic adjusting mechanisms are positioned at the upper ends of piston rods of the liquid storage pipes;
a liquid distribution valve mechanism for distributing solution is arranged in the middle of the side surface of the fixing frame, the bottom end of the liquid storage pipe is communicated with the top of the liquid distribution valve mechanism, a corrugated shaping pipe with adjusting and self-fixing functions is arranged at the outlet of the liquid distribution valve mechanism, a hose for conveying solution is inserted into the corrugated shaping pipe, a liquid injection mechanism for filling is fixedly arranged at the bottom end of the corrugated shaping pipe, the liquid injection mechanism is communicated with the liquid distribution valve mechanism through a hose, a switch used for controlling the liquid distribution valve mechanism is fixedly mounted on the outer side of the liquid injection mechanism, the tissue fixing mechanism is mounted on the outer side of the bottom end of the liquid injection mechanism in a plugging mode, a supporting table is mounted at the bottom of the side face of the fixing frame, an insulation box used for storing tissue samples is magnetically connected to the bottom of the supporting table, and a thermostat used for insulating the insulation box is arranged at the top of the supporting table.
Preferably, the liquid distribution valve mechanism comprises a liquid distribution pipe body, a hollow core rod is rotatably arranged in the liquid distribution pipe body, a stepping motor for driving the hollow core rod to rotate is arranged at the end part of the liquid distribution pipe body, a plurality of check valves matched with the bottom end of the liquid storage pipe are screwed at the top of the liquid distribution pipe body, and a plurality of communication holes which are arranged in a staggered mode and matched with outlets of the check valves are distributed on the outer surface of the hollow core rod.
In the invention, preferably, the fastening bracket is composed of two sets of elastic pieces with U-shaped structures, and the elastic pieces are clamped on the outer side of the liquid storage pipe.
Preferably, the hydraulic adjusting mechanism comprises a box body vertically connected with the guide groove in a sliding mode and a weight and counterweight piece placed inside the box body.
Preferably, the liquid injection mechanism comprises a handle part for holding, a sleeve part for matching with the tissue fixing mechanism and a needle head part for discharging liquid, the needle head part is installed at the bottom end of the sleeve part in a threaded connection mode, and a magnetic ring for fixing the tissue fixing mechanism is arranged between the handle part and the sleeve part.
Preferably, the tissue fixing mechanism comprises a main hoop which is sleeved with the liquid injection mechanism, the bottom of the main hoop is connected with a plurality of outwards-expanded elastic pieces, the outer side of each elastic piece is in threaded connection with an adjusting ring for restraining the elastic pieces, the bottom of each elastic piece is provided with a transition section matched with the needle head, the bottom end of each transition section is provided with a clamping end, and a plurality of fixing burrs are distributed on the surface of each clamping end.
Preferably, the heat preservation box comprises a liquid storage box body, handle grooves are formed in two ends of the liquid storage box body, two movable covers are connected to the top of the liquid storage box body in a magnetic attraction mode, and fixing grooves for fixing the tissue fixing mechanism are formed in the side faces of the movable covers.
Preferably, the section of the fixing seat is of an L-shaped structure, the upper surface of the fixing seat is provided with an overflow groove, a liquid draining plate is clamped inside the overflow groove, the top of the liquid draining plate is provided with a plurality of threaded through holes, one side of the fixing seat is provided with a hollow shell, the top of the hollow shell is provided with a negative pressure fan, the bottom of the hollow shell is provided with a waste liquid box through a hasp lock, an air inlet of the negative pressure fan extends to the bottom of the waste liquid box through a pipeline, a suction pipe is connected between the hollow shell and the overflow groove, and the bottom of the supporting table is further provided with a negative pressure fan cover connected with the suction pipe.
Compared with the prior art, the invention provides a perfusion fixing device for pathological sections, which has the following beneficial effects:
in the invention, a liquid injection mechanism, a liquid distribution valve mechanism and a tissue fixing mechanism are arranged, physiological saline and perfusion solution are respectively obtained by utilizing a plurality of liquid storage pipes, and the pressure of a hydraulic adjusting mechanism on a piston rod at the top of the liquid storage pipes is adjusted according to the variety of experimental animals and the difference of required tissue sample organs, so as to adjust the flow rate and perfusion pressure of the perfusion solution, ensure that the flow rate and pressure of the perfusion solution flowing out of the liquid injection mechanism are close to the body fluid flow of the experimental animals, protect the integrity of the tissue sample to the maximum extent, avoid damaging the internal structure and cells of the tissue sample, inject the physiological saline into an insulation box and place the insulation box on a support table to regulate and control the internal temperature of the insulation box, the liquid injection mechanism is supported by a corrugated shaping pipe and is suspended above the corrugated shaping pipe, and when the tissue sample is obtained, the external surface of the tissue sample is washed by the physiological saline, then the injection mechanism is inserted into the artery vessel of the tissue sample, meanwhile, the tissue fixing mechanism is clamped at the outer side of the artery vessel, the injection mechanism is fixed through the characteristic of a corrugated shaping pipe, the blood in the tissue sample is replaced by utilizing the physiological saline firstly, then the perfusate is switched by the fluid distribution valve mechanism to replace the physiological saline in the tissue sample, the tissue sample is perfused and fixed, the color of the tissue sample is observed, the tissue sample is taken down after the tissue sample is perfused and fixed, the tissue sample is transferred into the incubator by utilizing the tissue fixing mechanism to be suspended in the physiological saline and continuously replace the residual blood in the tissue sample, the fixing effect of the pathological characteristics in the tissue sample is enhanced, then the injection mechanism and the tissue fixing mechanism are separated, the compression on the inner surface and the outer surface of the tissue is avoided in the tissue sample obtaining process, and the injection mechanism and the tissue fixing mechanism are separated according to the size of the tissue sample, the type and the pertinence control of the perfusion flow and the pressure effectively improve the effect of fixing the pathological characteristics of the tissue sample, the perfusion process is convenient to control, and the operation of perfusion fixation is simplified.
Drawings
Fig. 1 is a schematic structural view of a perfusion fixing device for pathological sections according to the present invention;
FIG. 2 is a schematic view of an incubator of the perfusion fixing device for pathological section according to the present invention;
FIG. 3 is a schematic structural view of a liquid distribution valve mechanism of the perfusion fixing device for pathological sections according to the present invention;
FIG. 4 is a schematic structural view of an injection mechanism and a tissue fixing mechanism of the perfusion fixing device for pathological sections according to the present invention;
FIG. 5 is a schematic structural diagram of a liquid injection mechanism of the perfusion fixing device for pathological sections according to the present invention;
FIG. 6 is a schematic structural view of a tissue fixing mechanism of the perfusion fixing device for pathological section according to the present invention;
FIG. 7 is a schematic view of the open structure of the tissue fixing mechanism of the perfusion fixing device for pathological section according to the present invention;
fig. 8 is an enlarged structural view of a portion a of the perfusion fixing device for pathological sections according to the present invention;
fig. 9 is a schematic structural view of a fixing base of an infusion fixing device for pathological section according to embodiment 2 of the present invention.
In the figure: 1 fixed seat, 101 hollow shell, 102 waste liquid box, 103 negative pressure fan, 104 suction pipe, 105 liquid draining plate, 106 negative pressure fan cover, 2 fixed seat, 3 support table, 4 insulation box, 401 liquid storage box, 402 handle groove, 403 movable cover, 404 fixed groove, 5 liquid distributing valve mechanism, 501 liquid distributing pipe body, 502 stepping motor, 503 hollow core rod, 504 communication hole, 505 one-way valve, 6 liquid storage pipe, 7 fastening support, 8 liquid pressing adjusting mechanism, 9 guide groove, 10 corrugated shaping pipe, 11 liquid injecting mechanism, 1101 handle part, 1102 sleeve part, 1103 needle head part, 1104 ring, 12 tissue fixing mechanism, 1201 main hoop, 1202 elastic piece, 1203 transition section, 1204 clamping end, 1205 adjusting ring, 1206 fixed burr, and 13 switch.
Detailed Description
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
Example 1:
referring to fig. 1-8, a perfusion fixing device for pathological sections comprises a fixing seat 1 connected with external equipment, wherein a fixing frame 2 is installed above the fixing seat 1, a plurality of fastening brackets 7 are installed on the side surface of the fixing frame 2, a liquid storage tube 6 with an injector structure is installed in each fastening bracket 7 in a pluggable manner, a plurality of guide grooves 9 are further formed in the side surface of the fixing frame 2, a hydraulic adjusting mechanism 8 is installed in each guide groove 9 in a sliding manner, and the hydraulic adjusting mechanism 8 is located at the upper end of a piston rod of the liquid storage tube 6;
the side mid-mounting of mount 2 has the liquid valve mechanism 5 that joins in marriage that is used for distributing the solution, and the bottom of liquid storage pipe 6 and the top intercommunication of liquid valve mechanism 5 that joins in marriage, the ripple of possessing regulation and self-fixing function is established tub 10 in the export of liquid valve mechanism 5, and the inside grafting of ripple established tub 10 has the hose that is used for carrying the solution, the bottom fixed mounting of ripple established tub 10 has the notes liquid mechanism 11 that is used for pouring, annotate liquid mechanism 11 and join in marriage liquid valve mechanism 5 and pass through the hose intercommunication, the outside fixed mounting of annotating liquid mechanism 11 has the switch 13 that is used for controlling liquid valve mechanism 5 that joins in marriage, it installs tissue fixing mechanism 12 to annotate the bottom outside plug of liquid mechanism 11, a supporting bench 3 is installed to the side bottom of mount 2, and the bottom magnetism of supporting bench 3 is connected with the insulation can 4 that is used for depositing the tissue sample, the top of supporting bench 3 is provided with the heat retaining thermostat that is used for insulation can 4.
In the invention, a liquid injection mechanism 11, a liquid distribution valve mechanism 5 and a tissue fixing mechanism 12 are arranged, physiological saline and perfusion solution are respectively obtained by utilizing a plurality of liquid storage pipes 6, and the pressure of a pressure liquid adjusting mechanism 8 on a piston rod at the top of each liquid storage pipe 6 is adjusted according to the variety of experimental animals and the difference of required tissue sample organs, so as to adjust the flow rate and perfusion pressure of the perfusion solution, so that the flow rate and pressure of the perfusion solution flowing out of the liquid injection mechanism 11 are close to the body fluid flow of the experimental animals, the integrity of a tissue sample is protected to the maximum degree, the damage to the internal structure and cells of the tissue sample is avoided, the physiological saline is injected into an insulation box 4 and is placed on a support table 3, the internal temperature of the insulation box 4 is regulated, the liquid injection mechanism 11 is supported and suspended above by a corrugated shaping pipe 10, when the tissue sample is obtained, the external surface of the tissue sample is washed by the physiological saline, then the injection mechanism 11 is inserted into the artery vessel of the tissue sample, meanwhile, the tissue fixing mechanism 12 is clamped at the outer side of the artery vessel, the injection mechanism 11 is fixed through the characteristic of the corrugated shaping pipe 10, the blood in the tissue sample is replaced by the physiological saline firstly, then the perfusate is switched by the fluid distribution valve mechanism 5 to replace the physiological saline in the tissue sample, the tissue sample is perfused and fixed, the color of the tissue sample is observed, the tissue sample is taken down after perfusion and fixation, the tissue sample is transferred into the incubator 4 by the tissue fixing mechanism 12 to be suspended in the physiological saline, the residual blood in the tissue sample is continuously replaced, the fixing effect of pathological features in the tissue sample is enhanced, then the injection mechanism 11 and the tissue fixing mechanism 12 are separated, and the compression on the inner surface and the outer surface of the tissue is avoided in the tissue sample obtaining process, and according to the size and the type of the tissue sample, the perfusion flow and the perfusion pressure are controlled in a targeted manner, the fixing effect of the pathological characteristics of the tissue sample is effectively improved, the perfusion process is convenient to control, and the perfusion fixing operation is simplified.
As a further scheme of the present invention, the liquid distribution valve mechanism 5 includes a liquid distribution pipe 501, a hollow core rod 503 is rotatably installed inside the liquid distribution pipe 501, a step motor 502 for driving the hollow core rod 503 to rotate is installed at an end of the liquid distribution pipe 501, a plurality of check valves 505 matched with a bottom end of the liquid storage pipe 6 are screwed on a top of the liquid distribution pipe 501, a plurality of communication holes 504 which are arranged in a staggered manner and matched with outlets of the check valves 505 are distributed on an outer surface of the hollow core rod 503, in the present invention, when the hollow core rod 503 rotates to one of the communication holes 504 corresponds to the check valve 505, a solution of the matched liquid storage pipe 6 can flow into the liquid injection mechanism 11 through the hollow core rod 503, and each time an operator controls the step motor 502 to rotate through the switch 13, such that a circulation sequence of the solution can be adjusted, and a quick solution replacement effect can be achieved.
In the invention, after the liquid storage pipe 6 draws solution, the lower end of the liquid storage pipe 6 is inserted above the liquid distribution valve mechanism 5, the upper end of the liquid storage pipe is pushed into the fastening support 7 to be clamped and fixed, so that the liquid storage pipe 6 is in a vertical state, the upper end of the liquid storage pipe is in contact with the liquid pressing adjusting mechanism 8, the liquid storage pipe 6 can be quickly fixed, and the operation is simple and convenient.
As a further scheme in the invention, the hydraulic adjusting mechanism 8 comprises a box body vertically and slidably connected with the guide groove 9 and a weight counterweight part arranged in the box body, in the invention, the weight of the hydraulic adjusting mechanism 8 is used for pressing a piston rod of the liquid storage tube 6 to extrude the solution in the liquid storage tube 6, and when a tissue sample is cleaned, replaced and perfused, the weight in the box body is increased and decreased in a targeted manner according to the type of an experimental animal and the organ of the tissue sample, so that the flow rate and the pressure of the solution flowing out of the liquid injection mechanism 11 are accurately controlled during different operations of cleaning, replacing and perfusing the tissue sample, the damage to the internal structure and cells of the tissue sample during blood replacement and perfusion fixation is avoided, and the pathological characteristics of the tissue sample are maintained to the maximum extent.
As a further scheme of the present invention, the injection mechanism 11 includes a handle portion 1101 for holding, a sleeve portion 1102 for cooperating with the tissue fixing mechanism 12, and a needle portion 1103 for discharging liquid, the needle portion 1103 is mounted at the bottom end of the sleeve portion 1102 in a threaded manner, a magnetic ring 1104 for fixing the tissue fixing mechanism 12 is disposed between the handle portion 1101 and the sleeve portion 1102, in the present invention, a plurality of needle portions 1103 with different sizes are provided, the size and the type of the tissue sample are changed, and the tissue fixing mechanism 12 is directly sleeved outside the sleeve portion 1102, so that a worker operates the injection and the fixation of the tissue sample with one hand at the same time, and the operation is simple and convenient.
As a further proposal of the invention, the tissue fixing mechanism 12 comprises a main hoop 1201 sleeved with the injection mechanism 11, the bottom of the main hoop 1201 is connected with a plurality of elastic elements 1202 extending outwards, the outer side of the elastic elements 1202 is screwed with an adjusting ring 1205 for binding the elastic elements 1202, the bottom of the elastic elements 1202 is provided with a transition section 1203 matched with the needle head portion 1103, the bottom end of the transition section 1203 is provided with a clamping end 1204, and the surface of the clamping end 1204 is provided with a plurality of fixing burrs 1206.
As a further scheme of the present invention, the incubator 4 includes a liquid storage box 401, two ends of the liquid storage box 401 are provided with handle grooves 402, the top of the liquid storage box 401 is magnetically connected with two movable covers 403, and the side surfaces of the movable covers 403 are provided with fixing grooves 404 for fixing the tissue fixing mechanism 12, in the present invention, when a tissue sample is taken out, the tissue sample is suspended below the tissue fixing mechanism 12 through arterial blood vessels, in the process of transferring the tissue sample, the external surface of the tissue sample is not required to be pressed by using tools such as forceps, etc., the fixed tissue fixing mechanism 12 is inserted into the fixing grooves 404, the tissue sample can be suspended in physiological saline, meanwhile, after the tissue sample is transferred into the incubator 4, the tissue sample can be further filled and fixed, after the filling and fixing are completed, the liquid injection mechanism 11 can be taken out, and the damage to the external surface of the tissue sample in the process of transferring can be effectively avoided.
Example 2:
referring to fig. 1-9, a perfusion fixing device for pathological section, the section of a fixing base 1 is an L-shaped structure, the upper surface of the fixing base 1 is provided with an overflow groove, a draining plate 105 is clamped inside the overflow groove, the top of the draining plate 105 is provided with a plurality of threaded through holes, one side of the fixing base 1 is provided with a hollow shell 101, the top of the hollow shell 101 is provided with a negative pressure fan 103, the bottom of the hollow shell 101 is provided with a waste liquid box 102 through a snap lock, an air inlet of the negative pressure fan 103 extends to the bottom of the waste liquid box 102 through a pipeline, a suction pipe 104 is connected between the hollow shell 101 and the overflow groove, the bottom of the support table 3 is further provided with a negative pressure fan housing 106 connected with the suction pipe 104.
When the device is used, the liquid storage pipes 6 respectively obtain physiological saline and perfusion solution, the device can be used for adjusting the pressure of the pressure liquid adjusting mechanism 8 on the piston rods at the tops of the liquid storage pipes 6 according to the types of experimental animals and the difference of required tissue sample organs, further adjusting the flow rate and perfusion pressure of the perfusion solution, injecting the physiological saline into the thermal insulation box 4 and placing the physiological saline on the support table 3, adjusting and controlling the internal temperature of the thermal insulation box 4, the liquid injection mechanism 11 is supported and suspended above the corrugated shaping pipe 10, when the tissue sample is obtained, the external surface of the tissue sample is washed by the physiological saline, then the liquid injection mechanism 11 is inserted into an arterial blood vessel of the tissue sample, meanwhile, the tissue fixing mechanism 12 is clamped outside the arterial blood vessel, the liquid injection mechanism 11 is fixed by the characteristics of the corrugated shaping pipe 10, the tissue sample is replaced by the physiological saline, then the perfusion solution is switched by the liquid distribution valve mechanism 5, the physiological saline in the tissue sample is replaced, the tissue sample is perfused and fixed, the color of the tissue sample is observed, the tissue sample is taken down after the perfusion and the tissue sample is continuously suspended in the tissue sample, the residual tissue sample is fixed mechanism 12, and the residual tissue sample is obtained, and the tissue sample is continuously and the tissue sample is fixed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.
Claims (8)
1. A perfusion fixing device for pathological sections comprises a fixing seat (1) connected with external equipment, wherein a fixing frame (2) is installed above the fixing seat (1), and is characterized in that a plurality of fastening supports (7) are installed on the side surface of the fixing frame (2), a liquid storage pipe (6) of an injector structure is installed in the fastening supports (7) in a plugging mode, a plurality of guide grooves (9) are further formed in the side surface of the fixing frame (2), a hydraulic adjusting mechanism (8) is installed in the guide grooves (9) in a sliding mode, and the hydraulic adjusting mechanism (8) is located at the upper end of a piston rod of the liquid storage pipe (6);
the utility model discloses a liquid injection device, including mount (2), liquid storage pipe (6), bellows forming pipe (10) and liquid injection mechanism (11), the side mid-mounting of mount (2) has liquid distribution valve mechanism (5) that is used for distributing solution, and the bottom of liquid storage pipe (6) and the top intercommunication of liquid distribution valve mechanism (5), the export of liquid distribution valve mechanism (5) is installed and is possessed corrugated forming pipe (10) that adjust and from the fixed function, and the inside grafting of corrugated forming pipe (10) has the hose that is used for carrying solution, the bottom fixed mounting of corrugated forming pipe (10) has liquid injection mechanism (11) that is used for pouring, liquid injection mechanism (11) and liquid distribution valve mechanism (5) are through the hose intercommunication, the outside fixed mounting of liquid injection mechanism (11) has switch (13) that are used for controlling liquid distribution valve mechanism (5), tissue fixed establishment (12) is installed in the bottom outside plug of liquid injection mechanism (11), brace table (3) is installed to the side bottom of mount (2), and the bottom magnetism of brace table (3) is connected with insulation can (4) that are used for depositing the tissue sample, the top of brace table (3) is provided with the thermostat that is used for insulation can (4).
2. The perfusion fixing device for pathological sections as claimed in claim 1, wherein the liquid distribution valve mechanism (5) comprises a liquid distribution tube body (501), a hollow core rod (503) is rotatably installed inside the liquid distribution tube body (501), a stepping motor (502) for driving the hollow core rod (503) to rotate is installed at the end of the liquid distribution tube body (501), a plurality of check valves (505) matched with the bottom end of the liquid storage tube (6) are screwed on the top of the liquid distribution tube body (501), and a plurality of communication holes (504) which are arranged in a staggered mode and matched with the outlets of the check valves (505) are distributed on the outer surface of the hollow core rod (503).
3. Perfusion fixation device for pathological sections, according to claim 2, characterized in that the fastening bracket (7) is constituted by two sets of elastic members of U-shaped configuration, clamped on the outside of the reservoir tube (6).
4. Perfusion fixation device for pathological sections, according to claim 3, characterized in that the hydraulic adjustment mechanism (8) comprises a box in vertical sliding connection with the guide slot (9), and a counterweight placed inside the box.
5. The perfusion fixing device for pathological sections, according to claim 1, is characterized in that the injection mechanism (11) comprises a handle portion (1101) for holding, a socket portion (1102) for fitting the tissue fixing mechanism (12), and a needle portion (1103) for discharging liquid, the needle portion (1103) is mounted at the bottom end of the socket portion (1102) in a threaded manner, and a magnetic ring (1104) for fixing the tissue fixing mechanism (12) is arranged between the handle portion (1101) and the socket portion (1102).
6. The perfusion fixing device for pathological sections as claimed in claim 5, wherein the tissue fixing mechanism (12) comprises a main hoop (1201) sleeved with the liquid injection mechanism (11), the bottom of the main hoop (1201) is connected with a plurality of outwardly-expanded elastic members (1202), the outer side of the elastic members (1202) is screwed with an adjusting ring (1205) for restraining the elastic members (1202), the bottom of the elastic members (1202) is provided with a transition section (1203) matched with the needle head part (1103), the bottom end of the transition section (1203) is provided with a clamping end (1204), and a plurality of fixing burrs (1206) are distributed on the surface of the clamping end (1204).
7. The perfusion fixing device for pathological sections according to claim 6, wherein the incubator (4) comprises a liquid storage box (401), handle slots (402) are arranged at two ends of the liquid storage box (401), two movable covers (403) are magnetically connected to the top of the liquid storage box (401), and fixing slots (404) for fixing the tissue fixing mechanism (12) are arranged on the side surfaces of the movable covers (403).
8. The perfusion fixing device for pathological sections is characterized in that the section of the fixing seat (1) is of an L-shaped structure, an overflow groove is formed in the upper surface of the fixing seat (1), a liquid draining plate (105) is clamped inside the overflow groove, a plurality of threaded through holes are formed in the top of the liquid draining plate (105), a hollow shell (101) is installed on one side of the fixing seat (1), a negative-pressure fan (103) is arranged on the top of the hollow shell (101), a waste liquid box (102) is installed at the bottom of the hollow shell (101) through a snap lock, an air inlet of the negative-pressure fan (103) extends to the bottom of the waste liquid box (102) through a pipeline, a suction pipe (104) is connected between the hollow shell (101) and the overflow groove, and a negative-pressure fan cover (106) connected with the suction pipe (104) is further arranged at the bottom of the supporting platform (3).
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| CN202211554523.1A CN115979749B (en) | 2022-12-06 | 2022-12-06 | Perfusion fixing device for pathological section |
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| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
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| CN216509260U (en) * | 2021-12-16 | 2022-05-13 | 辽宁春光制药装备股份有限公司 | Double-layer cheese filling device |
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2022
- 2022-12-06 CN CN202211554523.1A patent/CN115979749B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203953871U (en) * | 2014-07-31 | 2014-11-26 | 王晓萃 | Experiment is poured into fixture with rat |
| CN206443022U (en) * | 2017-01-19 | 2017-08-29 | 昆明医科大学 | A kind of device for casting of experimental animal |
| CN112790188A (en) * | 2019-10-28 | 2021-05-14 | 西安交通大学医学院第一附属医院 | Rapid perfusion device for liver supply in liver transplantation |
| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
| CN114041454A (en) * | 2021-11-11 | 2022-02-15 | 首都医科大学附属北京安贞医院 | Tissue perfusion control method and device and perfusion system |
| CN216509260U (en) * | 2021-12-16 | 2022-05-13 | 辽宁春光制药装备股份有限公司 | Double-layer cheese filling device |
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