CN107115577A - A kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision - Google Patents
A kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision Download PDFInfo
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- CN107115577A CN107115577A CN201710283605.XA CN201710283605A CN107115577A CN 107115577 A CN107115577 A CN 107115577A CN 201710283605 A CN201710283605 A CN 201710283605A CN 107115577 A CN107115577 A CN 107115577A
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- 241000124008 Mammalia Species 0.000 title claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 101
- 230000010412 perfusion Effects 0.000 claims abstract description 94
- 239000008280 blood Substances 0.000 claims abstract description 43
- 210000004369 blood Anatomy 0.000 claims abstract description 43
- 238000004321 preservation Methods 0.000 claims abstract description 38
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 25
- 238000005266 casting Methods 0.000 claims abstract description 23
- 238000003860 storage Methods 0.000 claims abstract description 20
- 230000009469 supplementation Effects 0.000 claims abstract description 14
- 238000012806 monitoring device Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 70
- 239000002699 waste material Substances 0.000 claims description 19
- 238000013329 compounding Methods 0.000 claims description 17
- 239000007789 gas Substances 0.000 claims description 11
- 239000004020 conductor Substances 0.000 claims description 9
- 210000005240 left ventricle Anatomy 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 230000003028 elevating effect Effects 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 230000002463 transducing effect Effects 0.000 claims 1
- 238000002474 experimental method Methods 0.000 description 20
- 239000012153 distilled water Substances 0.000 description 9
- 210000005224 forefinger Anatomy 0.000 description 9
- 210000003813 thumb Anatomy 0.000 description 9
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical class CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 8
- 150000005693 branched-chain amino acids Chemical class 0.000 description 8
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000003053 toxin Substances 0.000 description 5
- 231100000765 toxin Toxicity 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 229940122388 Thrombin inhibitor Drugs 0.000 description 4
- 238000009530 blood pressure measurement Methods 0.000 description 4
- 230000000747 cardiac effect Effects 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 238000005429 filling process Methods 0.000 description 4
- 238000011010 flushing procedure Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000004088 simulation Methods 0.000 description 4
- 230000035488 systolic blood pressure Effects 0.000 description 4
- 229960003080 taurine Drugs 0.000 description 4
- 239000003868 thrombin inhibitor Substances 0.000 description 4
- 101000712605 Theromyzon tessulatum Theromin Proteins 0.000 description 3
- 229960004308 acetylcysteine Drugs 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 230000008602 contraction Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 230000004872 arterial blood pressure Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000000004 hemodynamic effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000007914 intraventricular administration Methods 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000004963 pathophysiological condition Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/14—Infusion devices, e.g. infusing by gravity; Blood infusion; Accessories therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M5/00—Devices for bringing media into the body in a subcutaneous, intra-vascular or intramuscular way; Accessories therefor, e.g. filling or cleaning devices, arm-rests
- A61M5/14—Infusion devices, e.g. infusing by gravity; Blood infusion; Accessories therefor
- A61M5/142—Pressure infusion, e.g. using pumps
- A61M5/14244—Pressure infusion, e.g. using pumps adapted to be carried by the patient, e.g. portable on the body
- A61M5/14276—Pressure infusion, e.g. using pumps adapted to be carried by the patient, e.g. portable on the body specially adapted for implantation
Landscapes
- Health & Medical Sciences (AREA)
- Vascular Medicine (AREA)
- Engineering & Computer Science (AREA)
- Anesthesiology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- External Artificial Organs (AREA)
Abstract
Experimental provision is irrigated the invention discloses a kind of Cardiological mammal isolated heart constant voltage/constant current, including fixed support device, device for casting and monitoring device, fixed support device includes base frame, fixed support plate and movable supporting plate are provided with rear side of base frame, the lower section of movable supporting plate is provided with hydraulic pressure lifting device, device for casting includes baffling and irrigates post, baffling perfusion post is fixed on the front of movable supporting plate, heart is connected with below baffling perfusion post, the lower section of heart is taper heat preservation tank and blood filter successively, oxygenator is connected with the right of blood filter, oxygenator passes through appendix respectively, liquid supplementation pipe and woven hose are connected with gas bomb, liquid storage bottle and peristaltic pump, the inlet that transfusion pipe end irrigates post with baffling is connected, baffling irrigates post, taper heat preservation tank is connected with thermostat by manifold, monitoring device passes through electrode, sacculus, phonocardio-transducer and pressure transducer gather and record test data.
Description
Technical field
The present invention relates to technical field of medical instruments, and in particular to a kind of Cardiological mammal isolated heart constant pressure/perseverance
Infusate flow experimental provision.
Background technology
Isolated working heart experiment is cardiovascular experiment common technology, can exclude the shadow of the systems such as nerve, breathing, endocrine
Ring, the reaction of observation heart for various environmental factors or drug factors in itself is the optimal of organ level during circulation science is tested
Experimental method.
The various isolated heart circulating perfusion devices that current prevalence is used, also make Langendorff Perfused isolated hearts fill
Put, the Perfused isolated heart system that big analog was initiated in 1895 by scientist Langendorff.It is dynamic suitable in vitro lactation
The measure of thing cardiac perfusion and isolated heart coronary flow, can directly carry out Constant pressure perfusion, and constant current filling can be carried out by adding peristaltic pump
Animal hearts are taken out in stream, Perfused isolated heart experiment, are connected to specific perfusion device, use perfusion perfusion, eliminate god
Through the control with body fluid, coordinate special analysis software record intraventricular pressure, arterial pressure and electrocardiosignal, automatically analyze every life
Parameter is managed, for the research such as the heart function under pathophysiological conditions and hemodynamic responses, in physiology, pathologic, physiologic and medicine
Widely applied in pharmacological research.
Such device its to constitute substantially be that, based on two glass perfusion posts, every perfusion post is fabricated to bilayer again, by
Snakelike borded pile and the thick post composition of straight, snakelike borded pile are available to the interior water passage of the perfusion liquid of heart, the thick post of straight
It is the outer water that the energy supplied by water bath with thermostatic control pump carries out the circulating distilled water of heating and thermal insulation to flowing perfusate in snakelike borded pile
Path；Other also include MariottoShi perfusions bottle, vacuum flask.In current Cardiovascular Disease Study field both at home and abroad, although this
Class isolated heart circulating perfusion device is that just possess before several centuries, but in the experiment of each emphasis cardiac electrophysiology stud
Room, is almost all carrying out respective experiment using Langendorff cardiac perfusions device as indispensable basic technology daily.Simply by
Complicated in initial perfusion device, cumbersome, the reason such as involve great expense, and is progressively simplified by later scholar, tamps, is modified to
Current in the market prevalence Langendorff cardiac perfusion devices, but device still carries two towering perfusion posts, also has above
MariottoShi perfusions bottle two big, sets up meter not science, and unreasonable structure, perfusion effect is poor, and measurement accuracy is low, and profile is not
Attractive in appearance, inconvenient operation, machine operation is unstable, and also have it is time-consuming, take liquid, lead to the drawbacks such as oxygen difficulty.
The content of the invention
For above technical problem, it is real that the present invention provides a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion
Experiment device.
The technical scheme is that：A kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision, its
It is characterised by, mainly including fixed support device, device for casting and monitoring device, the fixed support device includes base frame, consolidated
Determine supporting plate, movable supporting plate and hydraulic pressure lifting device, the center of the base frame is provided with stopper slot one, the stopper slot one
Right side is connected with stopper slot two by groove, and the lower section of base frame is provided with four supporting legs, and the fixed support plate has two, respectively
The right and left of the longitudinally fixed rear wall for being connected to base frame, the movable supporting plate is connected to two fixed supports by chute
Between plate, geometrical clamp of the front provided with an above-below direction of movable supporting plate, the hydraulic pressure lifting device is connected by elevating lever
The lower section of movable supporting plate is connected on, is fixed relative to iron stand and artificial fixed height, the present invention can precisely be determined by hydraulic pressure lifting device
Height, and then adjust perfusion pressure；The device for casting include baffling perfusion post, taper heat preservation tank, blood filter, oxygenator, gas bomb,
Liquid storage bottle, peristaltic pump and thermostat, the baffling perfusion post are fixedly connected on the front of movable supporting plate by the geometrical clamp
On, baffling perfusion post is double-decker, and the internal layer of baffling perfusion post is snakelike compared to traditional by the deflection plate that is vertically spaced
Pipe, is simple to manufacture and the perfusion liquid residence time is long so that water-bath heat transfer is more efficient；Baffling perfusion post upper right be provided with into
Liquid mouthful, the lower left of baffling perfusion post is provided with liquid outlet, and the upper and lower side in the left side of baffling perfusion post is respectively equipped with delivery port one
With water inlet one, pressure sensor sleeve pipe, the lower end of the pressure sensor sleeve pipe are threaded with below the liquid outlet
Heart is connected with by connector, the taper heat preservation tank is located at the lower section of the heart, and taper heat preservation tank is double-decker, cone
The bottom of shape heat preservation tank is provided with leting slip a remark, and the upper and lower side in the left side of taper heat preservation tank is respectively equipped with delivery port two and water inlet two, institute
Blood filter is stated in the stopper slot one, the left side of blood filter is connected by conduit with the oxygenator, and blood filter may filter that
Fall the toxin and waste in the perfusion liquid of backflow, as circulation fluid again after purification, can both save perfusion liquid, can also improve mould
The validity of plan and the accuracy for surveying data；The conduit is located in the groove, and oxygenator is located in the stopper slot two,
The inside of oxygenator is respectively equipped with appendix, liquid supplementation pipe and woven hose from right to left, and the appendix is connected with the gas bomb,
Appendix is provided with solenoid-operated proportional metering valve one, and the liquid supplementation pipe is connected with the liquid storage bottle, and liquid supplementation pipe is provided with solenoid-operated proportional
Metering valve two, liquid storage bottle is connected with the peristaltic pump and the inlet successively built with compounding perfusion liquid, the woven hose, institute
State the left side that thermostat is located at taper heat preservation tank, thermostat is provided with into water manifold and goes out water manifold, it is described enter water manifold difference
Be connected with the water inlet one and water inlet two, it is described go out water manifold be connected respectively with the delivery port one and delivery port two, lead to
Manifold is crossed to needing the baffling that water-bath is incubated to irrigate post and taper heat preservation tank Inlet and outlet water simultaneously, the circulation time of current can be shortened,
Avoid heat-energy losses；The monitoring device includes main frame, phonocardio-transducer, pressure transducer, electrode and sacculus, and the heart sound is changed
Energy device is fixed on the top of the fixed support plate on right side, and the electrode is attached to heart periphery and by insulated conductor for totally three respectively
It is connected with phonocardio-transducer, the pressure transducer is located at the lower section of phonocardio-transducer and is fixedly connected on the fixed support on right side
On plate, the sacculus be located at heart left ventricle and be connected by water pipe with the left end of pressure transducer, pressure transducer it is upper
Side is connected with syringe, and the main frame passes through wire and phonocardio-transducer, pressure transducer, temperature controller, blood filter, wriggling respectively
Pump is connected with hydraulic pressure lifting device.
Further, the lower end of the pressure sensor sleeve pipe is provided with two positions are relative, L-type notch in opposite direction,
The openend of the L-type notch is located at the lower edge of pressure sensor sleeve pipe, and pressure sensor sleeve pipe leads to the connector
Cross and buckle connection, buckle connection and compare threaded connection, operation more facilitates, especially by the heart connected and device for casting phase
When connecing, it can avoid significantly rotating damage heart when mounted.
Further, the connector is included in connection upper end, connection lower end, rubber sleeve and arc hand folder, the connection
The top at end is arranged with two relative short gears, and the connection lower end is structure as a whole with being connected upper end, connects the diameter of lower end
Less than connection upper end, connection lower end outer surface is provided with screw thread, and the lower end of rubber sleeve is fixedly connected under connection upper end and connection
The intersection at end, the rubber sleeve is isometric with being connected lower end, and arc hand folder totally two and is relatively fixed is connected to rubber respectively
The upper portion side wall of gum cover, short gear can be engaged with L-type notch, and the screw thread on connection lower end outer surface can make connector and heart
Arterial contact is more fastened, and operating personnel can be worn into arc hand folder by forefinger and thumb and strut rubber sleeve, be then enclosed on
It is fixed with being connected inside and outside lower end on the outer wall of arterial, one-hand operation, and also stability is high.
Further, the lower section of the blood filter is provided with sewer pipe, and the stopper slot one is run through in the upper end of the sewer pipe
Bottom is connected with blood filter, and the lower end of sewer pipe is connected with waste liquid bottle, and the waste liquid filtered out is flowed into waste liquid bottle by sewer pipe.
Further, it is provided with micropump, conduit and is closed close to the oxygen close to one end of the blood filter on the conduit
One end of device is provided with solenoid-operated proportional metering valve three, and solenoid-operated proportional metering valve three can control the ratio of circular treatment perfusion liquid.
Further, the use membrane oxygenator of the oxygenator preferably, specification is 1000-5000ml/min.
Further, position of the woven hose above the peristaltic pump is provided with three-way pipe, the right side of the three-way pipe
Side is connected with medicine-pouring pipe, and woven hose is provided with flow sensor close to the top of three-way pipe, and the top of the flow sensor is successively
Provided with oxygen concentration sensor and PH sensors, multi-party sensor can effectively monitor the parameters of filling process, and feed back in time,
The accuracy rate and success rate for the experiment that ensure that.
Further, the constituent of the compounding perfusion liquid includes by weight percentage：0.6-1.2%Nacl,
0.05-0.2Kcl, 0.025-0.035%Mgcl2.6H2O, 0.02-0.06%CaC1,0.2-0.5% glucose, 0.01-
0.02% taurine (Tau), 0.02-0.08% thrombin inhibitors, 0.01-0.014% acetylcysteines (NAC), 0.01-
0.016% branched-chain amino acid (BCAA), surplus is distilled water.
The present invention method of work step be：
Constant pressure is measured, the first step, and whole device is carried out into rinse one time first with compounding perfusion liquid, lower end then will be connected
In the arterial for inserting animal hearts, rubber sleeve is strutted while being worn using forefinger and thumb into arc hand folder, is then enclosed on dynamic
On the outer wall of vascular, then by it is short gear buckle into L-type notch, heart is rapidly secured on device for casting；
Second step, is raised to specified altitude assignment, by sustainer with 85cmH using hydraulic pressure lifting device by movable supporting plate2O pressure
After retroperfusion, recovering beat of heart, sacculus is inserted into heart left ventricle, then three electrodes are attached to heart periphery and by exhausted respectively
Edge wire is connected with phonocardio-transducer, and sacculus is connected by water pipe with pressure transducer, and left indoor pressure is recorded on computers, passes through
Syringe adjusts left room diastole internal pressure LVEDP between 4-10mmHg, after heart left chamber systolic pressure LVESP is stable and is more than
80mmHg, heart rate is more than 200 beats/min, can continue experiment, and record electrocardiogram ECG, aortic flow AF, coronary flow CF, a left side
Shrink the indexs such as end pressure LVESP in room；
3rd step, after experiment terminates, with the whole pipe-line system of distilled water flushing, prevents compounding perfusion liquid from remaining in pipeline
In.
Constant current is measured, and closes peristaltic pump, and remaining step is identical with constant pressure measurement.
Compared with prior art, beneficial effects of the present invention are：The Cardiological mammal isolated heart constant pressure of the present invention/
Constant current perfusion experimental provision can carry out constant voltage/constant current switching, and perfusate circulation and heat insulation effect are good.Wherein, it is of the invention
It is additionally arranged blood filter and may filter that toxin and waste in the perfusion liquid of backflow, as circulation fluid again after purification, can both saves
Perfusion liquid is saved, the validity of simulation can be also improved and survey the accuracy of data；Wherein, the internal layer of baffling perfusion post is between vertically
Every deflection plate, compared to traditional coiled pipe, be simple to manufacture and the perfusion liquid residence time be long so that water-bath heat transfer more added with
Effect；By manifold to needing the baffling that water-bath is incubated to irrigate post and taper heat preservation tank Inlet and outlet water simultaneously, the circulation of current can be shortened
Time, it is to avoid heat-energy losses；It is to buckle connection between the connector and device for casting of connection heart, compares threaded connection, operate
More facilitate, especially when the heart connected is connected with device for casting, it can avoid significantly rotating damage when mounted
Heart, wherein, operating personnel are worn into arc hand folder by forefinger and thumb and strut rubber sleeve, are then enclosed on the outer wall of arterial
On, complete with being connected inside and outside lower end fixed, one-hand operation, and stability is high.In a word, design science of the present invention, assembling are simultaneous
Tool is attractive in appearance and stably, and have the advantages that to operate be easy to, laborsaving and experiment success rate of saving worry and accuracy rate it is high.
Brief description of the drawings
Fig. 1 is the overall structure and annexation schematic diagram of the present invention；
Fig. 2 is the partial schematic diagram of the pressure sensor sleeve pipe of the present invention；
Fig. 3 is the structural representation of the connector of the present invention；
Fig. 4 is the rearview of the fixed support device of the present invention；
Fig. 5 is that the structure of the movable supporting plate of the present invention is schematic diagram；
Wherein, 1- fixed support devices, 11- base frames, 111- stopper slots one, 112- grooves, 113- stopper slots two, 114- branch
Leg, 12- fixed support plates, 13- movable supporting plates, 131- chutes, 132- geometrical clamps, 14- hydraulic pressure lifting devices, 141- elevating levers,
2- device for casting, 21- bafflings perfusion post, 211- inlets, 212- liquid outlets, 213- delivery ports one, 214- water inlets one, 215-
Pressure sensor sleeve pipe, 2151-L type grooves mouth, 216- connectors, 2161- connections upper end, 2162- connections lower end, 2163- rubber
Set, 2164- arcs hand folder, the short gears of 2165-, 217- woven hoses, 2171- three-way pipes, 2172- medicine-pouring pipes, 2173- flow sensings
Device, 2174- oxygen concentration sensors, 2175-PH sensors, 22- tapers heat preservation tank, 221- let slip a remark, 222- delivery ports two, 223- enter
The mouth of a river two, 23- blood filters, 231- conduits, 232- sewer pipes, 233- waste liquid bottles, 234- micropumps, 235- solenoid-operated proportional metering valves
3rd, 24- oxygenators, 25- gas bombs, 251- appendixs, 252- solenoid-operated proportionals metering valve one, 26- liquid storage bottles, 261- liquid supplementation pipes,
262- solenoid-operated proportionals metering valve two, 27- peristaltic pumps, 28- thermostats, 281- enter water manifold, 282- and go out water manifold, 3- monitoring dresses
Put, 31- main frames, 32- phonocardio-transducers, 33- pressure transducers, 34- electrodes, 35- sacculus, 36- insulated conductors, 37- water pipes,
38- syringes.
Embodiment
The present invention is further described in detail with reference to specific embodiment 1-5：
Embodiment 1：
As shown in Fig. 1,4,5, a kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision, its feature
It is, mainly include fixed support device 1, device for casting 2 and monitoring device 3, fixed support device 1 includes base frame 11, fixed
Supporting plate 12, movable supporting plate 13 and hydraulic pressure lifting device 14, the center of base frame 11 are provided with stopper slot 1, stopper slot one
111 right side is connected with stopper slot 2 113 by groove 112, and the lower section of base frame 11 is provided with four supporting legs 114, fixed support plate
12 have two, respectively the right and left of the longitudinally fixed rear wall for being connected to base frame 11, and movable supporting plate 13 passes through chute 131
It is connected between two fixed support plates 12, geometrical clamp 132 of the front provided with an above-below direction of movable supporting plate 13, liquid
Pressure lifter 14 is connected to the lower section of movable supporting plate 13 by elevating lever 141, is fixed relative to iron stand and artificial fixed height, this
Invention can precisely determine height by hydraulic pressure lifting device 14, and then adjust perfusion pressure；
As shown in figure 1, device for casting 2 includes baffling perfusion post 21, taper heat preservation tank 22, blood filter 23, oxygenator 24, storage
Gas cylinder 25, liquid storage bottle 26, peristaltic pump 27 and thermostat 28, baffling perfusion post 21 are fixedly connected on movable branch by geometrical clamp 132
On the front of fagging 13, baffling perfusion post 21 is double-decker, and the internal layer of baffling perfusion post 21 is by the deflection plate being vertically spaced, phase
Compared with traditional coiled pipe, it is simple to manufacture and the perfusion liquid residence time is long so that water-bath heat transfer is more efficient；Baffling irrigates post 21
Upper right be provided with inlet 211, baffling perfusion post 21 lower left be provided with liquid outlet 212, baffling irrigate post 21 a left side
The upper and lower side of side is respectively equipped with delivery port 1 and water inlet 1, and the lower section of liquid outlet 212 is threaded with pressure sensing
Device sleeve pipe 215, as shown in Fig. 2 the lower end of pressure sensor sleeve pipe 215 is provided with two positions are relative, L-type notch in opposite direction
2151, the openend of L-type notch 2151 is located at the lower edge of pressure sensor sleeve pipe 215, and pressure sensor sleeve pipe 215 is with connecting
Joint 216 buckles connection and compares threaded connection, operation more facilitates by buckling connection, especially by the heart connected with
When device for casting 2 connects, it can avoid significantly rotating damage heart when mounted.As shown in figure 3, connector 216 includes connecting
End 2161, connection lower end 2162, rubber sleeve 2163 and arc hand folder 2164 are connected, the top of connection upper end 2161 is arranged with two
Relative short gear 2165, connection lower end 2162 is structure as a whole with being connected upper end 2161, and the diameter of connection lower end 2162, which is less than, to be connected
End 2161 is connected, the connection outer surface of lower end 2162 is provided with screw thread, and the lower end of rubber sleeve 2163 is fixedly connected on connection upper end
2161 and the intersection of connection lower end 2162, rubber sleeve 2163 is isometric with being connected lower end 2162, arc hand press from both sides 2164 totally two and
The upper portion side wall for being connected to rubber sleeve 2163 is relatively fixed respectively, short gear 2165 can be engaged with L-type notch 2151, connect lower end
Screw thread on 2162 outer surfaces can be such that connector contacts with the arterial of heart more to fasten, and operating personnel can pass through forefinger and thumb
Finger wears into arc hand folder 2164 and struts rubber sleeve 2163, is then enclosed on the outer wall of arterial, with being connected inside and outside lower end 2162
It is fixed, one-hand operation, and also stability is high.The lower end of pressure sensor sleeve pipe 215 is connected with heart 4 by connector 216,
Taper heat preservation tank 22 is located at the lower section of heart 4, and taper heat preservation tank 22 is double-decker, and the bottom of taper heat preservation tank 22, which is provided with, lets slip a remark
221, the upper and lower side in the left side of taper heat preservation tank 22 is respectively equipped with delivery port 2 222 and water inlet 2 223, and blood filter 23 is located at limit
In the groove 1 of position, the left side of blood filter 23 is connected by conduit 231 with oxygenator 24, wherein, close to blood filter on conduit 231
23 one end is provided with micropump 234, conduit 231 is provided with solenoid-operated proportional metering valve 3 235, electromagnetism close to one end of oxygenator 24
Ratio metering valve 3 235 can control the ratio of circular treatment perfusion liquid.Wherein, the preferred use membrane oxygenator of oxygenator 24,
Specification is 1000ml/min.Blood filter 23 may filter that toxin and waste in the perfusion liquid of backflow, is used as and follows again after purification
Ring liquid, can both save perfusion liquid, can also improve the validity of simulation and survey the accuracy of data；The lower section of blood filter 23 is set
There is sewer pipe 232, the upper end of sewer pipe 232 is connected through the bottom of stopper slot 1 with blood filter 23, the lower end of sewer pipe 232
Waste liquid bottle 233 is connected with, the waste liquid filtered out is flowed into waste liquid bottle 233 by sewer pipe 232；Conduit 231 is located at groove 112
Interior, oxygenator 24 is located in stopper slot 2 113, and the inside of oxygenator 24 is respectively equipped with appendix 251, liquid supplementation pipe from right to left
261 and woven hose 217, appendix 251 is connected with gas bomb 25, and appendix 251 is provided with solenoid-operated proportional metering valve 1, mends
Liquid pipe 261 is connected with liquid storage bottle 26, and liquid supplementation pipe 261 is provided with solenoid-operated proportional metering valve 2 262, and liquid storage bottle 26 is filled built with compounding
Fluid injection, wherein, the constituent of compounding perfusion liquid includes by weight percentage：0.6%Nacl, 0.05Kcl,
0.025Mgcl2.6H2O, 0.02%CaC1,0.2% glucose, 0.01% taurine (Tau), 0.02% thrombin inhibitor,
0.01% acetylcysteine (NAC), 0.01% branched-chain amino acid (BCAA), surplus is distilled water.Woven hose 217 successively with it is compacted
Dynamic pump 27 is connected with inlet 211, wherein, woven hose 217 is provided with three-way pipe 2171, threeway close to the position of the top of peristaltic pump 27
The right side of pipe 2171 is connected with medicine-pouring pipe 2172, and woven hose 217 is provided with flow sensor 2173 close to the top of three-way pipe 2171,
The top of flow sensor 2173 is sequentially provided with oxygen concentration sensor 2174 and PH sensors 2175, and multi-party sensor can be supervised effectively
Survey the parameters of filling process, and feed back in time, it is ensured that experiment accuracy rate and success rate.Thermostat 28 is located at taper
The left side of heat preservation tank 22, thermostat 28 is provided with into water manifold 281 and water manifold 282 is gone out, enter water manifold 281 respectively with water inlet
One 214 are connected with water inlet 2 223, go out water manifold 281 and are connected respectively with delivery port 1 and delivery port 2 222, pass through manifold
To needing the baffling that water-bath is incubated to irrigate post 21 and taper heat preservation tank 22 Inlet and outlet water simultaneously, the circulation time of current can be shortened, kept away
Exempt from heat-energy losses；
As shown in figure 1, monitoring device 3 includes main frame 31, phonocardio-transducer 32, pressure transducer 33, electrode 34 and sacculus
35, phonocardio-transducer 32 is fixed on the top of the fixed support plate 12 on right side, and electrode 34 is attached to the periphery of heart 4 simultaneously for totally three respectively
It is connected by insulated conductor 36 with phonocardio-transducer 32, pressure transducer 33 is located at the lower section of phonocardio-transducer 32 and is fixedly connected
On the fixed support plate 12 on right side, sacculus 35 is located at the left ventricle of heart 4 and by a left side for water pipe 37 and pressure transducer 33
End is connected, and the top of pressure transducer 33 is connected with syringe 38, and main frame 31 passes through wire and phonocardio-transducer 32, pressure respectively
Transducer 33, temperature controller 28, blood filter 23, peristaltic pump 27 are connected with hydraulic pressure lifting device 14.
The present invention method of work step be：
Constant pressure is measured, the first step, and whole device is carried out into rinse one time first with compounding perfusion liquid, lower end then will be connected
In the arterial of 2162 insertion animal hearts 4, rubber sleeve is strutted while being worn using forefinger and thumb into arc hand folder 2164
2163, then it is enclosed on the outer wall of arterial, then buckled by short gear 2165 into L-type notch 2151, heart 4 is rapidly secured to
On device for casting 2；
Second step, is raised to specified altitude assignment, by sustainer with 85cmH2O using hydraulic pressure lifting device 14 by movable supporting plate 13
After pressure retroperfusion, recovering beat of heart, sacculus 35 is inserted into the left ventricle of heart 4, then three electrodes 34 are attached to outside heart 4 respectively
Week is simultaneously connected by insulated conductor 36 with phonocardio-transducer 32, and sacculus 35 is connected by water pipe 37 with pressure transducer 33, in electricity
Left indoor pressure is recorded on brain, left room diastole internal pressure LVEDP is adjusted in 4mmHg by syringe 38, heart left chamber systolic pressure is treated
After LVESP is stable and more than 80mmHg, heart rate is more than 200 beats/min, can continue experiment, and record electrocardiogram ECG, sustainer stream
Measure the indexs such as AF, coronary flow CF, left room contraction end pressure LVESP；
3rd step, after experiment terminates, with the whole pipe-line system of distilled water flushing, prevents compounding perfusion liquid from remaining in pipeline
In.
Constant current is measured, and closes peristaltic pump 27, and remaining step is identical with constant pressure measurement.
Embodiment 2：
As shown in Fig. 1,4,5, a kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision, its feature
It is, mainly include fixed support device 1, device for casting 2 and monitoring device 3, fixed support device 1 includes base frame 11, fixed
Supporting plate 12, movable supporting plate 13 and hydraulic pressure lifting device 14, the center of base frame 11 are provided with stopper slot 1, stopper slot one
111 right side is connected with stopper slot 2 113 by groove 112, and the lower section of base frame 11 is provided with four supporting legs 114, fixed support plate
12 have two, respectively the right and left of the longitudinally fixed rear wall for being connected to base frame 11, and movable supporting plate 13 passes through chute 131
It is connected between two fixed support plates 12, geometrical clamp 132 of the front provided with an above-below direction of movable supporting plate 13, liquid
Pressure lifter 14 is connected to the lower section of movable supporting plate 13 by elevating lever 141, is fixed relative to iron stand and artificial fixed height, this
Invention can precisely determine height by hydraulic pressure lifting device 14, and then adjust perfusion pressure；
As shown in figure 1, device for casting 2 includes baffling perfusion post 21, taper heat preservation tank 22, blood filter 23, oxygenator 24, storage
Gas cylinder 25, liquid storage bottle 26, peristaltic pump 27 and thermostat 28, baffling perfusion post 21 are fixedly connected on movable branch by geometrical clamp 132
On the front of fagging 13, baffling perfusion post 21 is double-decker, and the internal layer of baffling perfusion post 21 is by the deflection plate being vertically spaced, phase
Compared with traditional coiled pipe, it is simple to manufacture and the perfusion liquid residence time is long so that water-bath heat transfer is more efficient；Baffling irrigates post 21
Upper right be provided with inlet 211, baffling perfusion post 21 lower left be provided with liquid outlet 212, baffling irrigate post 21 a left side
The upper and lower side of side is respectively equipped with delivery port 1 and water inlet 1, and the lower section of liquid outlet 212 is threaded with pressure sensing
Device sleeve pipe 215, as shown in Fig. 2 the lower end of pressure sensor sleeve pipe 215 is provided with two positions are relative, L-type notch in opposite direction
2151, the openend of L-type notch 2151 is located at the lower edge of pressure sensor sleeve pipe 215, and pressure sensor sleeve pipe 215 is with connecting
Joint 216 buckles connection and compares threaded connection, operation more facilitates by buckling connection, especially by the heart connected with
When device for casting 2 connects, it can avoid significantly rotating damage heart when mounted.As shown in figure 3, connector 216 includes connecting
End 2161, connection lower end 2162, rubber sleeve 2163 and arc hand folder 2164 are connected, the top of connection upper end 2161 is arranged with two
Relative short gear 2165, connection lower end 2162 is structure as a whole with being connected upper end 2161, and the diameter of connection lower end 2162, which is less than, to be connected
End 2161 is connected, the connection outer surface of lower end 2162 is provided with screw thread, and the lower end of rubber sleeve 2163 is fixedly connected on connection upper end
2161 and the intersection of connection lower end 2162, rubber sleeve 2163 is isometric with being connected lower end 2162, arc hand press from both sides 2164 totally two and
The upper portion side wall for being connected to rubber sleeve 2163 is relatively fixed respectively, short gear 2165 can be engaged with L-type notch 2151, connect lower end
Screw thread on 2162 outer surfaces can be such that connector contacts with the arterial of heart more to fasten, and operating personnel can pass through forefinger and thumb
Finger wears into arc hand folder 2164 and struts rubber sleeve 2163, is then enclosed on the outer wall of arterial, with being connected inside and outside lower end 2162
It is fixed, one-hand operation, and also stability is high.The lower end of pressure sensor sleeve pipe 215 is connected with heart 4 by connector 216,
Taper heat preservation tank 22 is located at the lower section of heart 4, and taper heat preservation tank 22 is double-decker, and the bottom of taper heat preservation tank 22, which is provided with, lets slip a remark
221, the upper and lower side in the left side of taper heat preservation tank 22 is respectively equipped with delivery port 2 222 and water inlet 2 223, and blood filter 23 is located at limit
In the groove 1 of position, the left side of blood filter 23 is connected by conduit 231 with oxygenator 24, wherein, close to blood filter on conduit 231
23 one end is provided with micropump 234, conduit 231 is provided with solenoid-operated proportional metering valve 3 235, electromagnetism close to one end of oxygenator 24
Ratio metering valve 3 235 can control the ratio of circular treatment perfusion liquid.Wherein, the preferred use membrane oxygenator of oxygenator 24,
Specification is 3500ml/min.Blood filter 23 may filter that toxin and waste in the perfusion liquid of backflow, is used as and follows again after purification
Ring liquid, can both save perfusion liquid, can also improve the validity of simulation and survey the accuracy of data；The lower section of blood filter 23 is set
There is sewer pipe 232, the upper end of sewer pipe 232 is connected through the bottom of stopper slot 1 with blood filter 23, the lower end of sewer pipe 232
Waste liquid bottle 233 is connected with, the waste liquid filtered out is flowed into waste liquid bottle 233 by sewer pipe 232；Conduit 231 is located at groove 112
Interior, oxygenator 24 is located in stopper slot 2 113, and the inside of oxygenator 24 is respectively equipped with appendix 251, liquid supplementation pipe from right to left
261 and woven hose 217, appendix 251 is connected with gas bomb 25, and appendix 251 is provided with solenoid-operated proportional metering valve 1, mends
Liquid pipe 261 is connected with liquid storage bottle 26, and liquid supplementation pipe 261 is provided with solenoid-operated proportional metering valve 2 262, and liquid storage bottle 26 is filled built with compounding
Fluid injection, the constituent of compounding perfusion liquid includes by weight percentage：0.9%Nacl, 0.12%Kcl, 0.03%
Mgcl2.6H2O, 0.04%CaC1,0.25% glucose, 0.015% taurine (Tau), 0.06% thrombin inhibitor,
0.012% acetylcysteine (NAC), 0.013% branched-chain amino acid (BCAA), surplus is distilled water.Woven hose 217 successively with
Peristaltic pump 27 is connected with inlet 211, wherein, woven hose 217 is provided with three-way pipe 2171, three close to the position of the top of peristaltic pump 27
The right side of siphunculus 2171 is connected with medicine-pouring pipe 2172, and woven hose 217 is provided with flow sensor close to the top of three-way pipe 2171
2173, the top of flow sensor 2173 is sequentially provided with oxygen concentration sensor 2174 and PH sensors 2175, and multi-party sensor can
Effectively the parameters of monitoring filling process, and feed back in time, it is ensured that experiment accuracy rate and success rate.Thermostat 28
In the left side of taper heat preservation tank 22, thermostat 28 is provided with into water manifold 281 and water manifold 282 is gone out, enter water manifold 281 respectively with
Water inlet 1 is connected with water inlet 2 223, goes out water manifold 281 and is connected respectively with delivery port 1 and delivery port 2 222, leads to
Manifold is crossed to needing the baffling that water-bath is incubated to irrigate post 21 and taper heat preservation tank 22 Inlet and outlet water simultaneously, when can shorten the circulation of current
Between, it is to avoid heat-energy losses；
As shown in figure 1, monitoring device 3 includes main frame 31, phonocardio-transducer 32, pressure transducer 33, electrode 34 and sacculus
35, phonocardio-transducer 32 is fixed on the top of the fixed support plate 12 on right side, and electrode 34 is attached to the periphery of heart 4 simultaneously for totally three respectively
It is connected by insulated conductor 36 with phonocardio-transducer 32, pressure transducer 33 is located at the lower section of phonocardio-transducer 32 and is fixedly connected
On the fixed support plate 12 on right side, sacculus 35 is located at the left ventricle of heart 4 and by a left side for water pipe 37 and pressure transducer 33
End is connected, and the top of pressure transducer 33 is connected with syringe 38, and main frame 31 passes through wire and phonocardio-transducer 32, pressure respectively
Transducer 33, temperature controller 28, blood filter 23, peristaltic pump 27 are connected with hydraulic pressure lifting device 14.
The present invention method of work step be：
Constant pressure is measured, the first step, and whole device is carried out into rinse one time first with compounding perfusion liquid, lower end then will be connected
In the arterial of 2162 insertion animal hearts 4, rubber sleeve is strutted while being worn using forefinger and thumb into arc hand folder 2164
2163, then it is enclosed on the outer wall of arterial, then buckled by short gear 2165 into L-type notch 2151, heart 4 is rapidly secured to
On device for casting 2；
Second step, is raised to specified altitude assignment, by sustainer with 85cmH2O using hydraulic pressure lifting device 14 by movable supporting plate 13
After pressure retroperfusion, recovering beat of heart, sacculus 35 is inserted into the left ventricle of heart 4, then three electrodes 34 are attached to outside heart 4 respectively
Week is simultaneously connected by insulated conductor 36 with phonocardio-transducer 32, and sacculus 35 is connected by water pipe 37 with pressure transducer 33, in electricity
Left indoor pressure is recorded on brain, left room diastole internal pressure LVEDP is adjusted in 7mmHg by syringe 38, heart left chamber systolic pressure is treated
After LVESP is stable and more than 80mmHg, heart rate is more than 200 beats/min, can continue experiment, and record electrocardiogram ECG, sustainer stream
Measure the indexs such as AF, coronary flow CF, left room contraction end pressure LVESP；
3rd step, after experiment terminates, with the whole pipe-line system of distilled water flushing, prevents compounding perfusion liquid from remaining in pipeline
In.
Constant current is measured, and closes peristaltic pump 27, and remaining step is identical with constant pressure measurement.
Embodiment 3：
As shown in Fig. 1,4,5, a kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision, its feature
It is, mainly include fixed support device 1, device for casting 2 and monitoring device 3, fixed support device 1 includes base frame 11, fixed
Supporting plate 12, movable supporting plate 13 and hydraulic pressure lifting device 14, the center of base frame 11 are provided with stopper slot 1, stopper slot one
111 right side is connected with stopper slot 2 113 by groove 112, and the lower section of base frame 11 is provided with four supporting legs 114, fixed support plate
12 have two, respectively the right and left of the longitudinally fixed rear wall for being connected to base frame 11, and movable supporting plate 13 passes through chute 131
It is connected between two fixed support plates 12, geometrical clamp 132 of the front provided with an above-below direction of movable supporting plate 13, liquid
Pressure lifter 14 is connected to the lower section of movable supporting plate 13 by elevating lever 141, is fixed relative to iron stand and artificial fixed height, this
Invention can precisely determine height by hydraulic pressure lifting device 14, and then adjust perfusion pressure；
As shown in figure 1, device for casting 2 includes baffling perfusion post 21, taper heat preservation tank 22, blood filter 23, oxygenator 24, storage
Gas cylinder 25, liquid storage bottle 26, peristaltic pump 27 and thermostat 28, baffling perfusion post 21 are fixedly connected on movable branch by geometrical clamp 132
On the front of fagging 13, baffling perfusion post 21 is double-decker, and the internal layer of baffling perfusion post 21 is by the deflection plate being vertically spaced, phase
Compared with traditional coiled pipe, it is simple to manufacture and the perfusion liquid residence time is long so that water-bath heat transfer is more efficient；Baffling irrigates post 21
Upper right be provided with inlet 211, baffling perfusion post 21 lower left be provided with liquid outlet 212, baffling irrigate post 21 a left side
The upper and lower side of side is respectively equipped with delivery port 1 and water inlet 1, and the lower section of liquid outlet 212 is threaded with pressure sensing
Device sleeve pipe 215, as shown in Fig. 2 the lower end of pressure sensor sleeve pipe 215 is provided with two positions are relative, L-type notch in opposite direction
2151, the openend of L-type notch 2151 is located at the lower edge of pressure sensor sleeve pipe 215, and pressure sensor sleeve pipe 215 is with connecting
Joint 216 buckles connection and compares threaded connection, operation more facilitates by buckling connection, especially by the heart connected with
When device for casting 2 connects, it can avoid significantly rotating damage heart when mounted.As shown in figure 3, connector 216 includes connecting
End 2161, connection lower end 2162, rubber sleeve 2163 and arc hand folder 2164 are connected, the top of connection upper end 2161 is arranged with two
Relative short gear 2165, connection lower end 2162 is structure as a whole with being connected upper end 2161, and the diameter of connection lower end 2162, which is less than, to be connected
End 2161 is connected, the connection outer surface of lower end 2162 is provided with screw thread, and the lower end of rubber sleeve 2163 is fixedly connected on connection upper end
2161 and the intersection of connection lower end 2162, rubber sleeve 2163 is isometric with being connected lower end 2162, arc hand press from both sides 2164 totally two and
The upper portion side wall for being connected to rubber sleeve 2163 is relatively fixed respectively, short gear 2165 can be engaged with L-type notch 2151, connect lower end
Screw thread on 2162 outer surfaces can be such that connector contacts with the arterial of heart more to fasten, and operating personnel can pass through forefinger and thumb
Finger wears into arc hand folder 2164 and struts rubber sleeve 2163, is then enclosed on the outer wall of arterial, with being connected inside and outside lower end 2162
It is fixed, one-hand operation, and also stability is high.The lower end of pressure sensor sleeve pipe 215 is connected with heart 4 by connector 216,
Taper heat preservation tank 22 is located at the lower section of heart 4, and taper heat preservation tank 22 is double-decker, and the bottom of taper heat preservation tank 22, which is provided with, lets slip a remark
221, the upper and lower side in the left side of taper heat preservation tank 22 is respectively equipped with delivery port 2 222 and water inlet 2 223, and blood filter 23 is located at limit
In the groove 1 of position, the left side of blood filter 23 is connected by conduit 231 with oxygenator 24, wherein, close to blood filter on conduit 231
23 one end is provided with micropump 234, conduit 231 is provided with solenoid-operated proportional metering valve 3 235, electromagnetism close to one end of oxygenator 24
Ratio metering valve 3 235 can control the ratio of circular treatment perfusion liquid.Wherein, the preferred use membrane oxygenator of oxygenator 24,
Specification is 5000ml/min.Blood filter 23 may filter that toxin and waste in the perfusion liquid of backflow, is used as and follows again after purification
Ring liquid, can both save perfusion liquid, can also improve the validity of simulation and survey the accuracy of data；The lower section of blood filter 23 is set
There is sewer pipe 232, the upper end of sewer pipe 232 is connected through the bottom of stopper slot 1 with blood filter 23, the lower end of sewer pipe 232
Waste liquid bottle 233 is connected with, the waste liquid filtered out is flowed into waste liquid bottle 233 by sewer pipe 232；Conduit 231 is located at groove 112
Interior, oxygenator 24 is located in stopper slot 2 113, and the inside of oxygenator 24 is respectively equipped with appendix 251, liquid supplementation pipe from right to left
261 and woven hose 217, appendix 251 is connected with gas bomb 25, and appendix 251 is provided with solenoid-operated proportional metering valve 1, mends
Liquid pipe 261 is connected with liquid storage bottle 26, and liquid supplementation pipe 261 is provided with solenoid-operated proportional metering valve 2 262, and liquid storage bottle 26 is filled built with compounding
Fluid injection, wherein, the constituent of compounding perfusion liquid includes by weight percentage：1.2%Nacl, 0.2Kcl,
0.035Mgcl2.6H2O, 0.06%CaC1,0.5% glucose, 0.02% taurine (Tau), 0.08% thrombin inhibitor,
0.014% acetylcysteine (NAC), 0.016% branched-chain amino acid (BCAA), surplus is distilled water.Woven hose 217 successively with
Peristaltic pump 27 is connected with inlet 211, wherein, woven hose 217 is provided with three-way pipe 2171, three close to the position of the top of peristaltic pump 27
The right side of siphunculus 2171 is connected with medicine-pouring pipe 2172, and woven hose 217 is provided with flow sensor close to the top of three-way pipe 2171
2173, the top of flow sensor 2173 is sequentially provided with oxygen concentration sensor 2174 and PH sensors 2175, and multi-party sensor can
Effectively the parameters of monitoring filling process, and feed back in time, it is ensured that experiment accuracy rate and success rate.Thermostat 28
In the left side of taper heat preservation tank 22, thermostat 28 is provided with into water manifold 281 and water manifold 282 is gone out, enter water manifold 281 respectively with
Water inlet 1 is connected with water inlet 2 223, goes out water manifold 281 and is connected respectively with delivery port 1 and delivery port 2 222, leads to
Manifold is crossed to needing the baffling that water-bath is incubated to irrigate post 21 and taper heat preservation tank 22 Inlet and outlet water simultaneously, when can shorten the circulation of current
Between, it is to avoid heat-energy losses；
As shown in figure 1, monitoring device 3 includes main frame 31, phonocardio-transducer 32, pressure transducer 33, electrode 34 and sacculus
35, phonocardio-transducer 32 is fixed on the top of the fixed support plate 12 on right side, and electrode 34 is attached to the periphery of heart 4 simultaneously for totally three respectively
It is connected by insulated conductor 36 with phonocardio-transducer 32, pressure transducer 33 is located at the lower section of phonocardio-transducer 32 and is fixedly connected
On the fixed support plate 12 on right side, sacculus 35 is located at the left ventricle of heart 4 and by a left side for water pipe 37 and pressure transducer 33
End is connected, and the top of pressure transducer 33 is connected with syringe 38, and main frame 31 passes through wire and phonocardio-transducer 32, pressure respectively
Transducer 33, temperature controller 28, blood filter 23, peristaltic pump 27 are connected with hydraulic pressure lifting device 14.
The present invention method of work step be：
Constant pressure is measured, the first step, and whole device is carried out into rinse one time first with compounding perfusion liquid, lower end then will be connected
In the arterial of 2162 insertion animal hearts 4, rubber sleeve is strutted while being worn using forefinger and thumb into arc hand folder 2164
2163, then it is enclosed on the outer wall of arterial, then buckled by short gear 2165 into L-type notch 2151, heart 4 is rapidly secured to
On device for casting 2；
Second step, is raised to specified altitude assignment, by sustainer with 85cmH2O using hydraulic pressure lifting device 14 by movable supporting plate 13
After pressure retroperfusion, recovering beat of heart, sacculus 35 is inserted into the left ventricle of heart 4, then three electrodes 34 are attached to outside heart 4 respectively
Week is simultaneously connected by insulated conductor 36 with phonocardio-transducer 32, and sacculus 35 is connected by water pipe 37 with pressure transducer 33, in electricity
Left indoor pressure is recorded on brain, left room diastole internal pressure LVEDP is adjusted in 10mmHg by syringe 38, heart left chamber systolic pressure is treated
After LVESP is stable and more than 80mmHg, heart rate is more than 200 beats/min, can continue experiment, and record electrocardiogram ECG, sustainer stream
Measure the indexs such as AF, coronary flow CF, left room contraction end pressure LVESP；
3rd step, after experiment terminates, with the whole pipe-line system of distilled water flushing, prevents compounding perfusion liquid from remaining in pipeline
In.
Constant current is measured, and closes peristaltic pump 27, and remaining step is identical with constant pressure measurement.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that：It still may be used
To be modified to the technical scheme described in previous embodiment, or equivalent substitution is carried out to which part technical characteristic；And
These modifications are replaced, and the essence of appropriate technical solution is departed from the spirit and model of technical scheme of the embodiment of the present invention
Enclose.
Claims (7)
1. a kind of Cardiological mammal isolated heart constant voltage/constant current irrigates experimental provision, it is characterised in that main to include fixing
Support meanss (1), device for casting (2) and monitoring device (3), the fixed support device (1) include base frame (11), fixed support
Plate (12), movable supporting plate (13) and hydraulic pressure lifting device (14), the center of the base frame (11) are provided with stopper slot one
(111), the right side of the stopper slot one (111) is connected with stopper slot two (113), the lower section of base frame (11) by groove (112)
Provided with four supporting legs (114), the fixed support plate (12) has two, respectively the longitudinally fixed rear side for being connected to base frame (11)
The right and left of wall, the movable supporting plate (13) is connected between two fixed support plates (12) by chute (131), activity
Geometrical clamp (132) of the front of supporting plate (13) provided with an above-below direction, the hydraulic pressure lifting device (14) passes through elevating lever
(141) lower section of movable supporting plate (13) is connected to, the device for casting (2) includes baffling perfusion post (21), taper heat preservation tank
(22), blood filter (23), oxygenator (24), gas bomb (25), liquid storage bottle (26), peristaltic pump (27) and thermostat (28), described
Baffling perfusion post (21) is fixedly connected on the front of movable supporting plate (13) by the geometrical clamp (132), baffling perfusion post
(21) it is double-decker, the upper right of baffling perfusion post (21) is provided with inlet (211), and the lower section of baffling perfusion post (21) is left
Side is provided with liquid outlet (212), and the upper and lower side in the left side of baffling perfusion post (21) is respectively equipped with delivery port one (213) and water inlet one
(214) pressure sensor sleeve pipe (215), the pressure sensor sleeve pipe, are threaded with below the liquid outlet (212)
(215) lower end is connected with heart (4) by connector (216), and the taper heat preservation tank (22) is located under the heart (4)
Side, taper heat preservation tank (22) is double-decker, and the bottom of taper heat preservation tank (22), which is provided with, lets slip a remark (221), taper heat preservation tank (22)
The upper and lower side in left side be respectively equipped with delivery port two (222) and water inlet two (223), the blood filter (23) is located at described spacing
In groove one (111), the left side of blood filter (23) is connected by conduit (231) with the oxygenator (24), conduit (231) position
In the groove (112), oxygenator (24) is located in the stopper slot two (113), and the inside of oxygenator (24) is from right to left
It is respectively equipped with appendix (251), liquid supplementation pipe (261) and woven hose (217), the appendix (251) and the gas bomb (25)
It is connected, appendix (251) is provided with solenoid-operated proportional metering valve one (252), the liquid supplementation pipe (261) and the liquid storage bottle (26) phase
Even, liquid supplementation pipe (261) is provided with solenoid-operated proportional metering valve two (262), and liquid storage bottle (26) is built with compounding perfusion liquid, the transfusion
Pipe (217) is connected with the peristaltic pump (27) and the inlet (211) successively, and the thermostat (28) is located at taper heat preservation tank
(22) the left side, thermostat (28) is provided with into water manifold (281) and goes out water manifold (282), it is described enter water manifold (281) respectively
Be connected with the water inlet one (214) and water inlet two (223), it is described go out water manifold (281) respectively with the delivery port one
(213) it is connected with delivery port two (222), the monitoring device (3) includes main frame (31), phonocardio-transducer (32), pressure transducing
Device (33), electrode (34) and sacculus (35), the phonocardio-transducer (32) are fixed on the top of the fixed support plate (12) on right side,
The electrode (34) is attached to heart (4) periphery and is connected by insulated conductor (36) with phonocardio-transducer (32) for totally three respectively,
The pressure transducer (33) is located at the lower section of phonocardio-transducer (32) and is fixedly connected on the fixed support plate (12) on right side,
The sacculus (35) is located at the left ventricle of heart (4) and is connected by water pipe (37) with the left end of pressure transducer (33), pressure
Syringe (38) is connected with above transducer (33), the main frame (31) passes through wire and phonocardio-transducer (32), pressure respectively
Power transducer (33), temperature controller (28), blood filter (23), peristaltic pump (27) are connected with hydraulic pressure lifting device (14).
2. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as claimed in claim 1, its feature
It is, the lower end of the pressure sensor sleeve pipe (215) is provided with two positions are relative, L-type notch (2151) in opposite direction, institute
State L-type notch (2151) openend be located at pressure sensor sleeve pipe (215) lower edge, pressure sensor sleeve pipe (215 with
The connector (216) is by buckling connection.
3. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as described in claim 1,2, it is special
Levy and be, the connector (216) includes connection upper end (2161), connection lower end (2162), rubber sleeve (2163) and arc hand
Press from both sides (2164), the top of the connection upper end (2161) is arranged with two relative short gears (2165), the connection lower end
(2162) it is structure as a whole with being connected upper end (2161), the diameter of connection lower end (2162) is less than connection upper end (2161), connection
Lower end (2162) outer surface is provided with screw thread, and the lower end of rubber sleeve (2163) is fixedly connected under connection upper end (2161) and connection
The intersection of (2162) is held, the rubber sleeve (2163) is isometric with being connected lower end (2162), the arc hand presss from both sides (2164) totally two
It is individual and be relatively fixed the upper portion side wall for being connected to rubber sleeve (2163) respectively.
4. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as claimed in claim 1, its feature
It is, the lower section of the blood filter (23) is provided with sewer pipe (232), and the stopper slot is run through in the upper end of the sewer pipe (232)
One (111) bottom is connected with blood filter (23), and the lower end of sewer pipe (232) is connected with waste liquid bottle (233).
5. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as claimed in claim 1, its feature
It is, is provided with the conduit (231) close to one end of the blood filter (23) on micropump (234), conduit (231) close to institute
The one end for stating oxygenator (24) is provided with solenoid-operated proportional metering valve three (235).
6. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as described in claim 1,5, it is special
Levy and be, the preferred use membrane oxygenator of the oxygenator (24), specification is 1000-5000ml/min.
7. a kind of Cardiological mammal isolated heart constant voltage/constant current perfusion experimental provision as claimed in claim 1, its feature
It is, position of the woven hose (217) above the peristaltic pump (27) is provided with three-way pipe (2171), the three-way pipe
(2171) medicine-pouring pipe (2172) is connected with the right side of, woven hose (217) is provided with flow sensing close to the top of three-way pipe (2171)
Oxygen concentration sensor (2174) and PH sensors are sequentially provided with above device (2173), the flow sensor (2173)
(2175)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710283605.XA CN107115577B (en) | 2017-04-26 | 2017-04-26 | Experimental provision is perfused in a kind of Cardiological mammal isolated heart constant voltage/constant current |
Applications Claiming Priority (1)
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| CN108742875A (en) * | 2018-06-08 | 2018-11-06 | 新乡医学院 | A kind of high-efficiency in-vitro cardiac perfusion device |
| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
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| US20040219059A1 (en) * | 2003-05-03 | 2004-11-04 | Novosci Corp. | Heart bypass system incorporating minimized extracorporeal blood circulation system and related method of use |
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| CN112616830A (en) * | 2020-12-29 | 2021-04-09 | 亳州市人民医院 | Perfusion fixing system for pathological section |
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| CN107115577B (en) | 2018-06-19 |
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