CN103070736B - The perfusion fixture of toy experiment and method - Google Patents
The perfusion fixture of toy experiment and method Download PDFInfo
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- CN103070736B CN103070736B CN201310061398.5A CN201310061398A CN103070736B CN 103070736 B CN103070736 B CN 103070736B CN 201310061398 A CN201310061398 A CN 201310061398A CN 103070736 B CN103070736 B CN 103070736B
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Abstract
The present invention relates to perfusion fixture and the method for the experiment of a kind of toy, this device comprises base, base upper surface is provided with the first syringe holder and the second syringe holder, and it is corresponding with the first syringe holder to be provided with the first guide chute, be provided with the second guide chute corresponding with the second syringe holder, the first slide block is sliding combined with in first guide chute, the second slide block is sliding combined with in second guide chute, the first motor and the second motor is respectively equipped with in described base, first motor is connected with the first slide block by the first leading screw and nut mechanism, second motor is connected with the second slide block by the second leading screw and nut mechanism, first motor, second motor is all electrically connected circuit for controlling motor, base is provided with perfusion flow rate regulation button and motor control switch, perfusion flow rate regulation button and motor control switch are all electrically connected with circuit for controlling motor.Perfusion effect of the present invention is good, easy to operate, structure is simple.
Description
Technical field
The present invention relates to laboratory animal perfusion technique for fixing field, particularly relate to perfusion fixture and the method for the experiment of a kind of toy.
Background technology
In the research of tissues of experimental animals pathological experiment, particularly carry out in situ hybridization and immunohistochemical study, change for avoiding or reducing after death tissue morphology and structure, often before sacrifice of animal, adopt perfusion fixation.Perfusion is fixedly import in required fixing histoorgan by vascular routes by fixative, after being fixed rapidly in time in position by the cell of life, then wins sample.The tissue fixing through perfusion has certain hardness, and its all organizational structure maintains pre-neoplastic state, therefore select careful perfusion fixing be organized in form, the maintenance of organizational structure integrity has great importance.
Perfusion fixed form can divide heart and blood vessel (portal vein, Hepatic artery, femoral artery, ventral aorta etc.) two kinds perfusion according to the difference of fixative inlet.The organizational structure of each organ is different, can select different infusion liquid inlets, as liver can from portal vein entrance, lungs can from pulmonary artery entrance etc.General large animal is many by tremulous pulse, and toy can penetrate ventricle or aorta by direct entry needle, or adopts special device for casting.
The fixing many employing liquid gravity drops of usual perfusion carry out perfusion to be fixed, and the method for the multiplex manual injection of little individual animals is carried out perfusion and fixed, and up to now, the animal perfusion fixture minimum for body weight there is no report.Toy is as C57BL/6N and neonate rat body weight light (height 10-15cm, body weight 10-20g), and it organizes less, tender and lovely, if fixing bad, will cause difficulty of drawing materials.In addition, after sacrifice of animal supply of blood flow stops, organize especially brain and myeloid tissue very easily self-dissolving at short notice, therefore to guarantee rapidly at the solid fixed cycle operator of perfusion, fast, to keep the fresh and complete of organizational structure as far as possible, otherwise the time is slightly of a specified duration, to be there is a series of degeneration, necrosis in brain cell, affect the verity of experimental result, reliability and repeatability equally.
And the easy device adopting syringe or infusion bottle and entry needle to combine to carry out perfusion fixing, also there are problems in practice:
(1) the wayward pressure size of hand control syringe: when the easy device that perfusion fixation uses syringe and entry needle to combine operates, pressure is by operator's hand control, and when pressure is too small, perfusion effect is not good; When pressure is excessive, tissue damage (as angiorrhexis, heart bursts) can be caused.When the easy device using infusion bottle and entry needle to combine operates, can be suitable for rat and rabbit, but toy due to blood vessel this device pressure tiny obviously less than normal, can not perfusion effect be guaranteed.
(2) at least two people compounding practice is simultaneously needed during easy device operation: when the easy device using syringe and entry needle to combine operates, usually need more than two people compounding practice simultaneously, dissect perfusion, control syringe pressure respectively needs a people to come, and operation comparatively bothers, the imperfect of toy cerebral tissue structure may be caused, affect the verity of experimental result, reliability and repeatability.
Summary of the invention
An object of the present invention is the deficiency existed for prior art, the perfusion fixture that a kind of toy is tested is provided, it adopts a motor to drive normal saline flushing, another motor drives fixative perfusion, perfusion effect is good, easy to operate, structure is simple, and motor is by the full Based Intelligent Control of chip microcontroller, not only accurately can control the pressure poured into, and only need a people to dissect, pour into, operation rapidly, fast, time saving and energy saving and work efficiency is high, have a wide range of applications field.
Another object of the present invention is the deficiency existed for prior art, a kind of perfusion fixing means of the perfusion fixture adopting toy to test is provided, it is by the full Based Intelligent Control of chip microcontroller, not only accurately can control the pressure poured into, and the seriality of linking between normal saline flushing and fixative perfusion can be realized, improve the perfusion fixed effect of toy experiment.
First object of the present invention is achieved in that the perfusion fixture that a kind of toy is tested, comprise base, described base upper surface is provided with the first syringe holder and the second syringe holder, and it is corresponding with the first syringe holder to be provided with the first guide chute, be provided with the second guide chute corresponding with the second syringe holder, the first slide block is sliding combined with in first guide chute, the second slide block is sliding combined with in second guide chute, the first motor and the second motor is respectively equipped with in described base, described first motor is connected with the first slide block by the first leading screw and nut mechanism, described second motor is connected with the second slide block by the second leading screw and nut mechanism, described first motor, second motor is all electrically connected circuit for controlling motor, base is provided with perfusion flow rate regulation button and motor control switch, described perfusion flow rate regulation button and motor control switch are all electrically connected with circuit for controlling motor.
Described circuit for controlling motor comprises power supply, single-chip microcomputer, display circuit, instruction input circuit, the first stepper motor driver chip and the second stepper motor driver chip, described power supply is used for powering to single-chip microcomputer, display circuit, instruction input circuit, the first stepper motor driver chip and the second stepper motor driver chip, and the input and instruction input circuit of described single-chip microcomputer connects; First outfan of described single-chip microcomputer is connected with the input of the first stepper motor driver chip, and the outfan of described first stepper motor driver chip is connected with the first motor; Second outfan of described single-chip microcomputer is connected with the input of the second stepper motor driver chip, and the outfan of described second stepper motor driver chip is connected with the second motor; 3rd outfan of described single-chip microcomputer is connected with the input of display circuit.
The model of described single-chip microcomputer is STC89C52, and the model of described first stepper motor driver chip, the second stepper motor driver chip is L293D, and described display circuit adopts model to be the LCD MODULE of LCD1602.
Described first syringe holder and the second syringe holder fix the first syringe and the second syringe respectively, the front end of two syringes is by the tee T of joint connection one with catheter needle, the first end of described tee T is connected by the front end of joint with the first syringe, second end of described tee T is connected by the front end of joint with the second syringe, 3rd end of described tee T is connected with catheter needle by joint, and first and second end of described tee T is respectively equipped with switch.
The 3rd of described tee T is connected with filter by joint between end and catheter needle.
Described first syringe holder and the second syringe holder all comprise front holder and rear holder two parts, front holder and the rear holder upper end of the first syringe holder are equipped with locating slot, and front holder and the rear holder upper end of the second syringe holder are equipped with locating slot.
Described locating slot is semi-cylindrical recesses.
Described base is a casing, and described leading screw and nut mechanism one end is connected by shaft coupling with the output shaft of motor, the leading screw and nut mechanism other end by bearing and seat supports in casing.
Second object of the present invention is achieved in that the perfusion fixing means of the perfusion fixture adopting toy experiment, has the following steps:
1) first the syringe that normal saline is housed is separately fixed on the first syringe holder and the second syringe holder with the syringe that fixative is housed, tee T with catheter needle is connected with the syringe that fixative is housed with the syringe that normal saline is housed respectively, get rid of threeway inner air tube, then set the rate of flooding of normal saline and the rate of flooding of fixative respectively by perfusion flow rate regulation button;
2) close fixative perfusion switch on tee T, open saline infusions switch on tee T, press the motor control switch controlling saline infusions, perfusion normal saline, until blood is rinsed well, close saline infusions switch on tee T;
3) open the fixative perfusion switch of tee T, press the motor control switch controlling fixative perfusion, by setting rate of flooding perfusion fixative, until reach perfusion effect.
The rate of flooding of perfusion fixative adopts principle first quick and back slow, and namely, 10min speed is 4-5ml/min, and rear 10min speed is 2-3ml/min; The rate of flooding of rate of flooding by regulating perfusion flow rate regulation button to reduce fixative in filling process.
Beneficial effect of the present invention is: because perfusion fixture of the present invention adopts the tee T with catheter needle to be connected with the syringe that fixative is housed with the syringe that normal saline is housed respectively, motor is connected with the slide block be slidably fitted in guide chute by leading screw and nut mechanism, slide block is used for the piston handle of pushing syringe, and the syringe that normal saline is housed respectively is controlled separately by a motor with the syringe that fixative is housed, ensure that the rate of flooding of the rate of flooding of normal saline and fixative can be different, and first can pour into normal saline and rinse the blood in animal tissue well rear Reperfu-sion fixative, blood is avoided to affect the verity of experimental result.After on the holder syringe that normal saline is housed and syringe that fixative is housed being fixed on base upper surface, the pressure of syringe is controlled by motor, circuit for controlling motor, only needs a people to dissect and pours into, time saving and energy saving and work efficiency is high.
Two motors of the present invention are by stepper motor driver chip, Micro Controller Unit (MCU) driving, the rotating speed of motor, the position of stopping only depending on frequency and the umber of pulse of the pulse signal that stepper motor driver chip accepts, therefore, the perfusion flow rate regulation button that only need operate the instruction input circuit be connected with single-chip microcomputer can set the rate of flooding of normal saline and the rate of flooding of fixative respectively, thus accurately can control the injection pressure of normal saline and the injection pressure size of fixative and groundwater increment, what make to pour into is effective.
And the present invention can also according to the size adjustment injection pressure of perfusion animal and groundwater increment, therefore, the perfusion that the present invention is not only practically applicable to mice and neonate rat is fixed, and the perfusion being also applicable to the larger animal of the bodily forms such as rat is fixed, and has a wide range of applications field.
First and second end of described tee T of the present invention is respectively equipped with switch, liquid mixed flow can be prevented, thus avoid affecting perfusion effect.
The 3rd of described tee T of the present invention is connected with filter by joint between end and catheter needle, can filter the impurity in fixative, ensures perfusion effect.
The present invention also includes display circuit, can show the rate of flooding of two syringes and the duty of two motors.
After preparation, only need press corresponding motor control switch and can start to pour into normal saline or fixative, make the present invention easy to operate and rapidly, quick.In the work process of pouring into normal saline or fixative, first make the quick perfusion of fixative to whole tissue, reduced the rate of flooding of fixative subsequently by perfusion flow rate regulation button, fixative can be extended what organize and arrhea the time, fully reach fixing effect.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of apparatus of the present invention;
Fig. 2 is the structural representation of apparatus of the present invention;
Fig. 3 is the A direction view of Fig. 2;
Fig. 4 is that the B-B of Fig. 2 is to sectional view;
Fig. 5 is the schematic diagram of the circuit for controlling motor of apparatus of the present invention;
Fig. 6 is method flow diagram of the present invention.
In accompanying drawing, 1 is base, 2 is the first syringe holder, 3 is the second syringe holder, 4 is the first guide chute, 5 is the second guide chute, 6 is the first slide block, 7 is the second slide block, 8 is front holder, 9 is rear holder, 10 is locating slot, 11 is motor, 12 is leading screw and nut mechanism, 13 is bearing, 14 is bearing, 15 is tee T, 16 is catheter needle, 17 is the first switch, 18 is second switch, 19 is filter, 20 is joint, 21 is perfusion flow rate regulation button, 22 is motor control switch, 23 is the first syringe, 24 is the second syringe, 25 is LCDs.
Detailed description of the invention
See Fig. 1 to Fig. 5, it is a kind of embodiment of the perfusion fixture of toy experiment of the present invention.A kind of perfusion fixture of toy experiment, comprise base 1, described base 1 upper surface is provided with the first syringe holder 2 and the second syringe holder 3, and it is corresponding with the first syringe holder 2 to be provided with the first guide chute 4, be provided with the second guide chute 5 corresponding with the second syringe holder 3, be sliding combined with in first guide chute 4 in first slide block 6, second guide chute 5 and be sliding combined with the second slide block 7.Described first syringe holder 2 and the second syringe holder 3 all comprise front holder 8 and rear holder 9 two parts, front holder 8 and rear holder 9 upper end of the first syringe holder 2 are equipped with locating slot 10, and front holder 8 and rear holder 9 upper end of the second syringe holder 3 are equipped with locating slot 10.Described locating slot 10 is semi-cylindrical recesses.The first motor and the second motor is respectively equipped with in described base 1, described first motor is connected with the first slide block by the first leading screw and nut mechanism, described second motor is connected with the second slide block by the second leading screw and nut mechanism, described base 1 is a casing, described leading screw and nut mechanism 12 one end is connected by shaft coupling with the output shaft of motor 11, and leading screw and nut mechanism 12 other end is supported in casing by bearing 13 and bearing 14.
The first syringe 23 and the second syringe 24 fixed respectively by described first syringe holder 2 and the second syringe holder 3, two syringes 23, the front end of 24 connects one with the tee T 15 of catheter needle by joint 20, the first end of described tee T 15 is connected with the front end of the first syringe 23 by joint 20, second end of described tee T 15 is connected with the front end of the second syringe 24 by joint 20, 3rd end of described tee T 15 is connected with catheter needle 16 by joint 20, first of described tee T 15, two ends are respectively equipped with switch, be respectively the first switch 17 i.e. saline infusions switch and second switch 18 i.e. fixative perfusion switch, described first switch, second switch can prevent perfusion fluid mixed flow.Be connected with filter 19 by joint between 3rd end of the described tee T 15 of the present embodiment and catheter needle 16, effectively can consider foreign body in fixative and avoid affecting perfused tissue effect.Described catheter needle 16 is syringe needle, and syringe needle removes point, avoids causing accidental injury to animal hearts.Described first syringe holder 2 and the second syringe holder 3 are one group of syringe holder, the present invention can also arrange many group syringe holders on base 1, and guide chute, slide block, leading screw and nut mechanism, the motor corresponding with its syringe holder are set, described motor is controlled again by circuit for controlling motor, can realize the same time pours into branched animal, and charging efficiency is high.
Described first motor U5, the second motor U6 are all electrically connected circuit for controlling motor, base 1 is provided with four perfusion flow rate regulation buttons 21 and two motor control switch 22, and described four perfusion flow rate regulation buttons 21 and two motor control switch 22 are all electrically connected with circuit for controlling motor.Described circuit for controlling motor comprises power supply, single-chip microcomputer U1, display circuit, instruction input circuit, the first stepper motor driver chip U2 and the second stepper motor driver chip U3, described power supply is used for powering to single-chip microcomputer U1, display circuit, instruction input circuit, the first stepper motor driver chip U2 and the second stepper motor driver chip U3, the input and instruction input circuit of described single-chip microcomputer U1 connects, for receiving the instruction input of instruction input circuit.Described instruction input circuit comprises four perfusion flow rate regulation buttons 21 and four resistance, four perfusion flow rate regulation buttons 21 are respectively the first turbo SB1, the first deceleration button SB2, the second turbo SB3, the second deceleration button SB4, and four resistance are respectively the first resistance R1, the second resistance R2, the 3rd resistance R3, the 4th resistance R4.Described perfusion flow rate regulation button 21, for the rate of flooding of the rate of flooding and fixative that set normal saline, can carry out accelerating and slowing down.First outfan of described single-chip microcomputer U1 is connected with the input of the first stepper motor driver chip U2, for exporting pulse control signal to the first stepper motor driver chip U2, the outfan of described first stepper motor driver chip U2 is connected with the first motor U5, for driving the first motor U5 according to pulse control signal, control stroke and the rotary speed of the first motor U5.Second outfan of described single-chip microcomputer U1 is connected with the input of the second stepper motor driver chip U3, for exporting pulse control signal to the second stepper motor driver chip U3, the outfan of described second stepper motor driver chip U3 is connected with the second motor U6, for driving the second motor U6 according to pulse control signal, control stroke and the rotary speed of the second motor U6.Two motor control switch 22 are respectively the first motor control switch, the second motor control switch, described first motor control switch between the first motor and power supply, for controlling the start and stop of the first motor.Second motor control switch between the second motor and power supply, for controlling the start and stop of the second motor.Described first motor control switch is the motor control switch controlling saline infusions, and described second motor control switch is the motor control switch controlling fixative perfusion.First motor U5 and the second motor U6 of the present embodiment are four phase step motor, and four phase step motor has five interfaces, are respectively first interface, the second interface, the 3rd interface, the 4th interface, the 5th interface.3rd outfan of described single-chip microcomputer U6 is connected with the input of display circuit, for serial data and control signal are passed to display circuit, for showing the duty of the first syringe, the rate of flooding of the second syringe and the first motor, the second motor.
The present embodiment employing model is the single-chip microcomputer STC89C52 of U1, XTAL1, XTAL2 of described U1 are connected with crystal oscillating circuit, described crystal oscillating circuit comprises crystal oscillator X, the first electric capacity C1, the second electric capacity C2, the P3.4 of described U1 is connected with warning circuit, for pouring into the warning in flow process at the end of flow process, described warning circuit comprises the 5th resistance R5, PNP triode VZ1 and attention device Bell.Described in the present embodiment, the model of the first stepper motor driver chip U2, the second stepper motor driver chip U3 is L293D, described display circuit adopts model to be the LCD MODULE U4 of LCD1602, and the LCDs 25 of described LCD MODULE U4 is arranged on a sidewall of base 1.The physical circuit of the present embodiment connects as follows:
See Fig. 5, the 3rd P1.3 inputting IN3, described U1 of the P1.2 connection U2 of second input IN2, the described U1 of the P1.1 connection U2 of first input IN1, the described U1 of the P1.0 connection U2 of described U1 connects the 4th of U2 and inputs IN4, 3rd P1.7 inputting IN3, described U1 of the P1.6 connection U2 of second input IN2, the described U1 of the P1.5 connection U2 of first input IN1, the described U1 of the P1.4 connection U3 of described U1 connects the 4th of U2 and inputs IN4, the P2.0 of described U1 connects the 7th pin of U4, the P2.1 of described U1 connects the 8th pin of U4, the P2.2 of described U1 connects the 9th pin of U4, the P2.3 of described U1 connects the 10th pin of U4, the P2.4 of described U1 connects the 11st pin of U4, the P2.5 of described U1 connects the 12nd pin of U4, the P2.6 of described U1 connects the 13rd pin of U4, the P2.7 of described U1 connects the 14th pin of U4, the P3.6 of described U1 connects the 4th pin of U4, the P3.7 of described U1 connects the 6th pin of U4, 15th pin of described U4 is all connected power supply VCC with the 2nd pin, 16th pin of described U4 and the equal ground connection of the 1st pin, 3rd pin of described U4 is by variable resistance R6 ground connection, described crystal oscillator X is connected on the two ends of XTAL1, XTAL2 of U1, and the first electric capacity C1 is connected between XTAL1 and ground, and the second electric capacity C2 is connected between XTAL2 and ground, described first turbo SB1 is connected between the P3.0 of U1 and ground, first deceleration button SB2 is connected between the P3.1 of U1 and ground, second turbo SB3 is connected between the P3.2 of U1 and ground, second deceleration button SB4 is connected between the P3.3 of U1 and ground, described first resistance R1 is connected between the P3.0 of U1 and power supply VCC, second resistance R2 is connected between the P3.1 of U1 and power supply VCC, 3rd resistance R3 is connected between the P3.2 of U1 and power supply VCC, and the 4th resistance R4 is connected between the P3.3 of U1 and power supply VCC, described 5th resistance R5 is connected between the P3.4 of U1 and the base stage of PNP triode VZ1, and alarm Bell is connected between the emitter stage of PNP triode VZ1 and power supply VCC, the grounded collector of described PNP triode VZ1, the Vcc of described U1 connects power supply VCC, the GND ground connection of described U1.
First of U2 exports the 5th interface that OUT1 connects the first motor U5, second of U2 exports the 4th interface that OUT2 connects the first motor U5, the 3rd of U2 exports the 3rd interface that OUT3 connects the first motor U5, the 4th of U2 exports the second interface that OUT4 connects the first motor U5, the first interface of the first motor U5 connects power supply VCC, the equal ground connection of GND pin of U2, VSS, E1 of U2 are all connected power supply VCC with E2 pin, the VS pin of U2 connects power supply 12V, for driving U2; First of U3 exports the 5th interface that OUT1 connects the second motor U6, second of U3 exports the 4th interface that OUT2 connects the second motor U6, the 3rd of U3 exports the 3rd interface that OUT3 connects the second motor U6, the 4th of U3 exports the second interface that OUT4 connects the second motor U6, the first interface of the second motor U6 connects power supply VCC, the equal ground connection of GND pin of U3, VSS, E1 of U3 are all connected power supply VCC with E2 pin, the VS pin of U3 connects power supply 12V, for driving U3.
See Fig. 6, for adopting the flow chart of the perfusion fixing means of the perfusion fixture of toy experiment, have the following steps:
1) first the first syringe that normal saline is housed is separately fixed on the first syringe holder and the second syringe holder with the second syringe that fixative (4% paraformaldehyde) is housed, tee T with catheter needle is connected with the second syringe that fixative is housed with the first syringe that normal saline is housed respectively, close the saline infusions switch on the first end of tee T, open the fixative perfusion switch on the second end of tee T, perfusion flow velocity is regulated to fill second of tee T fast by perfusion flow rate regulation button, three ends, then the fixative perfusion switch on the second end of tee T is closed, open the saline infusions switch on the first end of tee T, until normal saline flows out rear saline infusions switch of closing tee T first end from perfusion syringe needle, get rid of threeway inner air tube, the rate of flooding of normal saline and the rate of flooding of fixative is set respectively again by perfusion flow rate regulation button.The in-built normal saline 20ml of first syringe described in the present embodiment is fixative 20ml in the second syringe.
2) be fixed on a cystosepiment by lying on the back again after Animal Anesthesia, open thoracic cavity, expose heart, an osculum is cut at the apex of the heart (left ventricle) to the left with eye scissors, reeve ascending aorta is thrust from the left apex of the heart gently according to anatomical structure with catheter needle, after fixing with ophthalmic tweezers, cut off right auricle, open the saline infusions switch on the first end of tee T, press the first motor control switch controlling saline infusions, start the first motor, perfusion normal saline, until blood is rinsed well, close the saline infusions switch on the first end of tee T.The speed of the perfusion normal saline of the present embodiment setting is 4-5ml/min.
3) when the liquid flowed out until animal right atrium becomes colorless liquid from scarlet blood, open the fixative perfusion switch on the second end of tee T, press motor second gauge tap controlling fixative perfusion, start the second motor, by setting rate of flooding perfusion fixative, until reach perfusion effect, after reaching perfusion effect, extract catheter needle, carry out tissue sampling.The rate of flooding of perfusion fixative adopts principle first quick and back slow, and namely, 10min speed is 4-5ml/min, and rear 10min speed is 2-3ml/min; The rate of flooding of rate of flooding by regulating perfusion flow rate regulation button to reduce fixative in filling process.
The perfusion that apparatus of the present invention and method are not only practically applicable to mice and neonate rat is fixed, and the perfusion being also applicable to the larger animal of the bodily forms such as rat is fixed, and has a wide range of applications field.
Claims (6)
1. the perfusion fixture of a toy experiment, comprise base, it is characterized in that: described base upper surface is provided with the first syringe holder and the second syringe holder, and it is corresponding with the first syringe holder to be provided with the first guide chute, be provided with the second guide chute corresponding with the second syringe holder, the first slide block is sliding combined with in first guide chute, the second slide block is sliding combined with in second guide chute, the first motor and the second motor is respectively equipped with in described base, described first motor is connected with the first slide block by the first leading screw and nut mechanism, described second motor is connected with the second slide block by the second leading screw and nut mechanism, described first motor, second motor is all electrically connected circuit for controlling motor, base is provided with perfusion flow rate regulation button and motor control switch, described perfusion flow rate regulation button and motor control switch are all electrically connected with circuit for controlling motor, perfusion flow rate regulation button sets the rate of flooding of normal saline and the rate of flooding of fixative respectively, motor control switch for controlling saline infusions controls the start and stop of the first motor, and the motor control switch for controlling fixative perfusion controls the start and stop of the second motor,
Described first syringe holder and the second syringe holder are fixed for dress the first syringe of normal saline and the second syringe for filling fixative respectively, the front end of two syringes is by the tee T of joint connection one with catheter needle, the first end of described tee T is connected by the front end of joint with the first syringe, second end of described tee T is connected by the front end of joint with the second syringe, 3rd end of described tee T is connected with catheter needle by joint, first of described tee T, two ends are respectively equipped with saline infusions switch and fixative perfusion switch, the 3rd of described tee T is connected with filter by joint between end and catheter needle.
2. the perfusion fixture of toy experiment according to claim 1, it is characterized in that: described circuit for controlling motor comprises power supply, single-chip microcomputer, display circuit, instruction input circuit, the first stepper motor driver chip and the second stepper motor driver chip, described power supply is used for powering to single-chip microcomputer, display circuit, instruction input circuit, the first stepper motor driver chip and the second stepper motor driver chip, and the input and instruction input circuit of described single-chip microcomputer connects; First outfan of described single-chip microcomputer is connected with the input of the first stepper motor driver chip, and the outfan of described first stepper motor driver chip is connected with the first motor; Second outfan of described single-chip microcomputer is connected with the input of the second stepper motor driver chip, and the outfan of described second stepper motor driver chip is connected with the second motor; 3rd outfan of described single-chip microcomputer is connected with the input of display circuit.
3. the perfusion fixture of toy experiment according to claim 2, it is characterized in that: the model of described single-chip microcomputer is STC89C52, the model of described first stepper motor driver chip, the second stepper motor driver chip is L293D, and described display circuit adopts model to be the LCD MODULE of LCD1602.
4. the perfusion fixture of toy experiment according to claim 1, it is characterized in that: described first syringe holder and the second syringe holder all comprise front holder and rear holder two parts, front holder and the rear holder upper end of the first syringe holder are equipped with locating slot, and front holder and the rear holder upper end of the second syringe holder are equipped with locating slot.
5. the perfusion fixture of toy experiment according to claim 4, is characterized in that: described locating slot is semi-cylindrical recesses.
6. the perfusion fixture of toy experiment according to claim 1, it is characterized in that: described base is a casing, one end of described first leading screw and nut mechanism, the second leading screw and nut mechanism is connected by shaft coupling with the output shaft of the first motor, the second motor respectively, the other end of the first leading screw and nut mechanism, the second leading screw and nut mechanism respectively by bearing and seat supports in casing.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105854661B (en) * | 2016-06-17 | 2018-11-13 | 四川大学 | A kind of automatic preparation facilities and its working method of confection |
| CN107551344B (en) * | 2017-10-13 | 2023-07-14 | 中国人民解放军总医院 | Rapid drug delivery device and method |
| CN109596610A (en) * | 2018-12-29 | 2019-04-09 | 莱茵技术（上海）有限公司 | Content of formaldehyde rapid detection method and device in a kind of textile |
| CN112616830B (en) * | 2020-12-29 | 2022-01-25 | 亳州市人民医院 | Perfusion fixing system for pathological section |
| CN112891013B (en) * | 2021-01-27 | 2023-08-25 | 中国人民解放军空军军医大学 | Device for injecting medicine into colorectal of small rodent experimental animal |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE232060T1 (en) * | 1996-09-10 | 2003-02-15 | Omrix Biopharm Sa | DEVICE FOR APPLYING A MULTI-COMPONENT LIQUID |
| US6248093B1 (en) * | 1998-10-29 | 2001-06-19 | Minimed Inc. | Compact pump drive system |
| CN2388959Y (en) * | 1999-09-30 | 2000-07-26 | 夏黎明 | CT intensified high-pressure long distance injection apparatus |
| CN200948276Y (en) * | 2006-08-30 | 2007-09-19 | 徐州天荣医疗通讯设备有限公司 | Pushing and pulling synchronous type pill ejector jet pump |
| CN201394094Y (en) * | 2009-04-23 | 2010-02-03 | 首都医科大学 | Device for small-scale test animal perfusion fixation |
| CN201798973U (en) * | 2010-09-28 | 2011-04-20 | 白娟娟 | Fixed-flow syringe allowing flow adjustment |
| CN201912562U (en) * | 2010-12-02 | 2011-08-03 | 张友念 | Intelligent roll booster |
| CN202069956U (en) * | 2011-04-28 | 2011-12-14 | 张明东 | Micro syringe for oncology department |
| CN203183083U (en) * | 2013-02-27 | 2013-09-11 | 中国人民解放军第三军医大学 | Perfusion fixation device of small animal experiments |
-
2013
- 2013-02-27 CN CN201310061398.5A patent/CN103070736B/en not_active Expired - Fee Related
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