CN105277592A - Temperature-control detachable bath for configuration of field stimulating electrode and application method thereof - Google Patents
Temperature-control detachable bath for configuration of field stimulating electrode and application method thereof Download PDFInfo
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- CN105277592A CN105277592A CN201510642392.6A CN201510642392A CN105277592A CN 105277592 A CN105277592 A CN 105277592A CN 201510642392 A CN201510642392 A CN 201510642392A CN 105277592 A CN105277592 A CN 105277592A
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Abstract
The invention relates to a detachable temperature-control bath for configuration of a field stimulating electrode and an application method thereof. The bath comprises a full water jacket and a bath. The full water jacket is provided with a temperature-control perfusion inlet and a temperature-control perfusion outlet. An electrode assembly and an oxygen inlet are arranged in the bath. The electrode assembly comprises a positive electrode and a negative electrode which are arranged opposite to each other in the vertical direction. The full water jacket and the bath are separately arranged and can be individually placed in any temperature environment required by experiments for testing after separated from each other according to experiment needs. The detachable temperature-control bath for configuration of the field stimulating electrode has advantages of compact and concise design, low cost and no higher requirement on electrode material, is suitable for popularization and application, is easy to dismount and replace and is compatible with experiment requirements on different capacities and temperature demands.
Description
Technical field:
The present invention relates to a kind of detachable bath of temperature control and the using method thereof that configure a stimulating electrode, belong to the technical field of in vitro tissue or organ experiment.
Background technology:
In modern medicine, biology especially Neuroscience Research, in vitro tissue or the analysis of organ Muscle tensility are one of experimental techniques of extremely important and widespread use.It is physiology, and the research of the subjects such as pharmacology provides the function assessment research evidence of organ-tissue level, can combine with other researchs from molecular cell level and whole animal level, the high level of the Study of the Realization and comprehensive.The research of nerve regulation effect is the important topic during in vitro tissue or organ functional activity are studied; This research requires that, outside observation initiatively shrink tension (namely without any electro photoluminescence, only giving certain mechanical preload), bestowing extraneous electric fields stimulates, simulated tissue nerve stimulation.
But the experimental technique at present giving electrical field stimulation in vitro tissue or organ is the pattern that is separated with bath body of electrode mainly.Namely external stationary electric field stimulating electrode is needed.There is following defect in this experimental provision: there is serious occupy-place phenomenon after 1, external electrode structure thick (needing L-type fixed support) enters the bath of low capacity above bath, for follow-up experimental implementation as, the tissue more renewed or organ, dosing observation etc. increase difficulty, very easily interference is caused to experiment, affect the stable of result and reliability; 2, external electrode needs to fix separately, and easily changes with the relative position of in vitro tissue and organ, need often adjust, and affects the stable of result, extends the Preparatory work of experiment time, reduce the service efficiency of tested sample; 3, in narrow and small bath, fragile electrode when organizing is changed; If change the bath of larger space, overall body lotion volume must be made to increase, then result change unreliable when needing the effect of observing trace drug.
Article " application of the flat bath of micro-many water guides in combination of Chinese tradiational and Western medicine research " (Wei Mu new No.1 Attached Hospital, Nanjing Medical Univ department of traditional Chinese medicine) discloses the inconvenience that a kind of horizontal bath brings to solve external electrode, but at present mostly laboratory uses vertical type bath, the horizontal bath of replacing cause the waste of vertical type bath and cost larger.Electrical field stimulation the problems referred to above that experiment exists are given based in vitro tissue or organ, can only carry out to the aforesaid active contraction movement of simple observation in routine experimentation education, the research learning giving electrical field stimulation project cannot be related to, bring obstruction to experiment and study comprehensively.
How the electrode of thicker electrical field stimulation is installed in narrow and small bath, ensures the stable of experiment and can repeat; Simplify laboratory operating procedures, reduce the loss of electrode, and make bath have higher versatility, become a problem demanding prompt solution.
Summary of the invention:
The invention provides a kind of detachable temperature control bath configuring a stimulating electrode.This temperature control bath structure is simple, and versatility is high, is applicable in the study of the routine experimentation such as scientific research and student education.
The invention still further relates to a kind of using method of above-mentioned detachable temperature control bath.
Term illustrates:
Bath: the Muscle tensility analysis being mainly used in the in vitro tissue of acute isolation or organ (flesh bar as from stomach, enteron aisle, tracheae, blood vessel etc.) measures.In vitro tissue or organ can maintain physiologically active in the bath being full of oxygen saturation kreb ' s body lotion (implantation environment), stimulate by imposing extraneous electric fields to it, can realize its Muscle tensility contraction movement and the experimental provision how being subject to nerve regulation research.
Technical scheme of the present invention is as follows:
Configure a detachable temperature control bath for a stimulating electrode, comprise full water jacket and bath, bath is removably disposed in full water jacket; Described full water jacket is provided with temperature control perfusion entrance and the outlet of temperature control perfusion; Electrode group and oxygen intake is provided with in described bath; Described electrode group comprises positive electrode and negative electrode; Described positive electrode and negative electrode are vertically oppositely arranged.Full water jacket is separated setting with bath, after can experimentally needing bath to be separated with full water jacket, tests under being placed on separately the temperature environment of any requirement of experiment.
Preferably, described electrode group comprises multipair positive electrode and negative electrode; Each electrode component is not connected with independent gauge tap.The advantage herein designed is, can experimentally need, and opens and closes the electrode of diverse location, and to adapt to tissue or the organ sample specification of separate sources, guarantee that sample can obtain effective electric field and stimulate, improve experiment degree of accuracy and variation, versatility is higher.
Further preferred, throughly on the sidewall of described bath be provided with electrode contact, described positive electrode and negative electrode are hinged with electrode contact respectively; Described electrode contact is electrically connected with stimulator.Electrode and electrode contact hinged, after experimentally requiring selected positive and negative electrode, can turn up not needing the electrode opened, concordant with described bath madial wall, avoid the interference to experimental implementation, improve conventional efficient, be convenient to observe.
Preferably, the very semiclosed arc-shaped structure of described positive electrode and negative electricity; Described bath comprises bath body and bottom; Described bottom is dismountable to be arranged on bottom bath body.Semiclosed arc-shaped structure not only avoid electrode affects tension detection with direct contact of tested tissue, and also assures that tested tissue receives effective electrical field stimulation, semi-closed structure ensures that aftermentioned replacing organizational process is simple and easy to do.The demountable structure of bath is convenient to be installed on electrode and the assembling of experimental provision by tested tissue, and facilitates the clean and maintenance of bath.
Preferred further, described bottom is provided with hook and drainage tube; Described full water jacket is provided with the through hole matched with described drainage tube; Drainage tube is through to be arranged in described through hole; Drainage tube is connected with drainage output control system.Drainage tube coordinates with through hole the fixation also played bath.
Preferred further, described oxygen intake is arranged on bottom; Bottom is evenly provided with venthole.Oxygen intake being arranged on bottom is beneficial in the liquid that oxygen is fully dissolved in bath, and in addition, the venthole evenly arranged is beneficial to being uniformly distributed of oxygen.
Preferably, described oxygen intake is arranged on the sidewall of bath, and described oxygen intake is consistent with the height of bottommost electrode.The sidewall that oxygen intake is arranged on bath ensures change tested sample, dosing and change the experimental implementation such as liquid have larger operating space, avoid electrode damage or disturbance records.
Preferably, be provided with multiple electrode group in described bath, described multiple electrode groups are evenly arranged in bath.Can according to the tissue of separate sources or organ sample specification, the ideal position of adjustment electrical field stimulation, improves conventional efficient flexibly.
Preferably, the diameter of described bath is 12mm, is highly 50mm, is respectively 19mm and 11mm bottom bath with the distance of described positive electrode, negative electrode vertical direction; Bottom described oxygen intake and bath, the distance of vertical direction is 11mm.The international standard of tested sample is 10mm × 5mm; The tested sample of guarantee that arranges so is just in time in the effective electric field stimulation of 2 electrodes formation and can't contacts electrode.
A using method for above-mentioned temperature control bath, comprises step as follows:
1) bath is separated with full water jacket, opens bottom; Tested sample is hung over and links up with above, by tested sample successively through positive and negative electrode, be connected with tonotransducer; If change tested sample to be difficult to operation from bath top, therefore adopt detachable bottom;
2) bottom is connected with bath body; Described hook is alignd at vertical direction with electrode group;
3) in bath, fill body lotion, fill warm water between full water jacket and bath, temperature control perfusion entrance and the outlet of temperature control perfusion are by being connected with temperature control perfusion system; The temperature of described warm water is 36 DEG C ~ 37 DEG C;
4) by the temperature of temperature control perfusion system regulation and control bath environment;
5) electric stimulation operation is carried out, the change of test in vitro tissue shrink tension amplitude.
The advantage of invention is:
1, the detachable temperature control bath of configuration field of the present invention stimulating electrode, by being configured with the detachable bath of electrical field stimulation electrode, realizes experimental implementation (as changed sample, changing dressings) simple, can not interference experiment record, improves conventional efficient; Save lab space, ensure the requirement of experiment of low capacity background, operating space is unobstructed, avoids and touches electrode;
2, the detachable temperature control bath of configuration field of the present invention stimulating electrode, sets each some locations parameter according to current international practice sample-size, ensures that tissue to be measured can accept consistent extraneous electric fields and stimulate, guarantees that experimental result is stablized;
3, the detachable temperature control bath of configuration field of the present invention stimulating electrode, succinctly compact to design, with low cost, to electrode material without high request, is suitable for promoting the use of; Easy dismounting and change can the requirement of experiment of compatible different capabilities and temperature requirements.
Accompanying drawing illustrates:
Fig. 1 is the structural representation of the detachable temperature control bath of configuration field of the present invention stimulating electrode;
Fig. 2 is the structural representation of bath of the present invention;
Fig. 3 is the structural representation of bottom of the present invention;
Wherein, 1, positive electrode; 2, negative electrode; 3, bottom; 4, full water jacket; 5, drainage tube; 6, temperature control perfusion entrance; 7, temperature control perfusion outlet; 8, link up with; 9, oxygen intake; 10, stimulator; 11, oxygen supply pipe; 12, bath body; 13, tonotransducer; 14, electrode contact.
Embodiment:
Below in conjunction with embodiment and Figure of description, invention is described in detail, but is not limited thereto.
As Figure 1-3.
Embodiment 1,
Configure a detachable temperature control bath for a stimulating electrode, comprise full water jacket 4 and bath, bath is removably disposed in full water jacket 4; Described full water jacket 4 is provided with temperature control perfusion entrance 6 and temperature control perfusion outlet 7; Electrode group and oxygen intake 9 is provided with in described bath; Described electrode group comprises positive electrode 1 and negative electrode 2; Described positive electrode 1 and negative electrode 2 are vertically oppositely arranged; Described positive electrode and negative electricity be stainless steel (also can be other materials such as weary polarization filamentary silver or platinum filament) very.Full water jacket 4 is separated setting with bath, after can experimentally needing bath to be separated with full water jacket 4, tests under being placed on separately the temperature environment of any requirement of experiment.
Embodiment 2,
The detachable temperature control bath of configuration field as described in Example 1 stimulating electrode, its difference is, described electrode group comprises multipair positive electrode 1 and negative electrode 2; Each electrode component is not connected with independent gauge tap.The advantage herein designed is, adapts to tissue or the organ sample specification of separate sources, and guarantee that sample can obtain effective electric field stimulation and improve experiment degree of accuracy and variation, versatility is higher.
Embodiment 3,
The detachable temperature control bath of configuration field as described in Example 2 stimulating electrode, its difference is, throughly on the sidewall of described bath is provided with electrode contact 14, and described positive electrode 1 and negative electrode 2 are hinged with electrode contact respectively; Described electrode contact 14 is electrically connected with stimulator 10.Electrode and electrode contact 14 hinged, after experimentally requiring selected positive and negative electrode, can turn up not needing the electrode opened, concordant with described bath madial wall, avoid the interference to experimental implementation, improve conventional efficient, be convenient to observe.
Embodiment 4,
The detachable temperature control bath of configuration field as described in Example 1 stimulating electrode, its difference is, described positive electrode 1 and negative electrode 2 are semiclosed arc-shaped structure; Described bath comprises bath body 12 and bottom 3; Described bottom 3 is dismountable to be arranged on bottom bath body 12.Semiclosed arc-shaped structure not only avoid electrode and direct contact of tested tissue and affects tension detection, also assures that tested tissue receives effective electrical field stimulation, and it is simple and easy to do that semi-closed structure ensures to change organizational process.The demountable structure of bath is convenient to be installed on electrode and the assembling of experimental provision by tested tissue, and facilitates the clean and maintenance of bath.
Embodiment 5,
The detachable temperature control bath of configuration field as described in Example 4 stimulating electrode, its difference is, described bottom 3 is provided with hook 8 and drainage tube 5; Described full water jacket 4 is provided with the through hole matched with described drainage tube 5; Drainage tube 5 is through to be arranged in described through hole; Drainage tube 5 is connected with drainage output control system, and described drainage output control system is PLC system, is controlled the output of liquid in bath, temperature test etc. by PLC system.Drainage tube 5 coordinates with through hole the fixation also played bath.
Embodiment 6,
The detachable temperature control bath of configuration field as described in Example 4 stimulating electrode, its difference is, described oxygen intake 9 is arranged on bottom 3; Bottom 3 is evenly provided with venthole.Oxygen intake 9 being arranged on bottom is beneficial in the liquid that oxygen is fully dissolved in bath, and in addition, the venthole evenly arranged is beneficial to being uniformly distributed of oxygen.
Embodiment 7,
The detachable temperature control bath of configuration field as described in Example 1 stimulating electrode, its difference is, described oxygen intake 9 is arranged on the sidewall of bath, and described oxygen intake 9 is consistent with the height of bottommost electrode.The sidewall that oxygen intake 9 is arranged on bath ensures change tested sample, dosing and change the experimental implementation such as liquid have larger operating space, avoid electrode damage or disturbance records.Described oxygen intake 9 is consistent with the height of bottommost electrode, ensures that whole tested tissue fully can absorb oxygen.
Embodiment 8,
The detachable temperature control bath of configuration field as described in Example 1 stimulating electrode, its difference is, is provided with 3 electrode groups in described bath, and 3 described electrode groups are evenly arranged on the madial wall in bath, and each electrode group height is consistent.Can according to the tissue of separate sources or organ sample specification, the ideal position of adjustment electrical field stimulation, improves conventional efficient flexibly.
Embodiment 9,
The detachable temperature control bath of configuration field as described in Example 1 stimulating electrode, its difference is, the diameter of described bath is 15mm, is highly 50mm, is respectively 19mm and 11mm bottom bath with the distance of described positive electrode 1, negative electrode 2 vertical direction; Bottom described oxygen intake 9 and bath, the distance of vertical direction is 11mm.The international standard of tested sample is 10mm*5mm; The tested sample of guarantee that arranges so is just in time in the effective electric field stimulation of 2 electrodes formation and can't contacts electrode.
Embodiment 10,
The using method of the detachable temperature control bath of the configuration field stimulating electrode as described in embodiment 1-9 any one, comprises step as follows:
1) bath is separated with full water jacket 4, opens bottom 3; Tested sample is hung over after on hook 8, by tested sample successively through positive and negative electrode, be connected with tonotransducer 13; If change tested sample to be difficult to operation from bath top, therefore adopt detachable bottom;
2) bottom 3 is connected with bath body 12; Described hook 8 is alignd at vertical direction with electrode group;
3) in bath, fill body lotion, fill warm water between full water jacket 4 and bath, temperature control perfusion entrance 6 and the outlet 7 of temperature control perfusion are by being connected with temperature control perfusion system; The temperature of described warm water is 37 DEG C;
4) by the temperature of temperature control perfusion system regulation and control bath environment;
5) electric stimulation operation is carried out, the change (unit: g) of test in vitro tissue shrink tension amplitude.Method of testing is as follows accordingly: selected parameters of electrical stimulation is, 50V extra-high pressure, and frequency 8Hz, 0.5ms stimulate time-histories, and continuing stimulates 5s, and interval 70s repeats once; Each stimulation can bring out typical biphasic reaction, namely of short duration diastole heel significantly shrinks with one, this contraction is acknowledged as the generation of neurogenic acetylcholine nervous excitation, so this changes in amplitude of shrinking of observable judges whether medicine has effect to neurogenic acetylcholine nerve regulation.
Claims (10)
1. configure a detachable temperature control bath for a stimulating electrode, comprise full water jacket and bath, bath is removably disposed in full water jacket; It is characterized in that, described full water jacket is provided with temperature control perfusion entrance and the outlet of temperature control perfusion; Electrode group and oxygen intake is provided with in described bath; Described electrode group comprises positive electrode and negative electrode; Described positive electrode and negative electrode are vertically oppositely arranged.
2. the detachable temperature control bath of configuration field as claimed in claim 1 stimulating electrode, it is characterized in that, described electrode group comprises multipair positive electrode and negative electrode; Each electrode component is not connected with independent gauge tap.
3. the detachable temperature control bath of configuration field as claimed in claim 2 stimulating electrode, is characterized in that, throughly on the sidewall of described bath is provided with electrode contact, and described positive electrode and negative electrode are hinged with electrode contact respectively; Described electrode contact is electrically connected with stimulator.
4. the detachable temperature control bath of configuration field as claimed in claim 1 stimulating electrode, is characterized in that, the very semiclosed arc-shaped structure of described positive electrode and negative electricity; Described bath comprises bath body and bottom; Described bottom is dismountable to be arranged on bottom bath body.
5. the detachable temperature control bath of configuration field as claimed in claim 4 stimulating electrode, is characterized in that, described bottom is provided with hook and drainage tube; Described full water jacket is provided with the through hole matched with described drainage tube; Drainage tube is through to be arranged in described through hole; Drainage tube is connected with drainage output control system.
6. the detachable temperature control bath of configuration field as claimed in claim 4 stimulating electrode, it is characterized in that, described oxygen intake is arranged on bottom; Bottom is evenly provided with venthole.
7. the detachable temperature control bath of configuration field as claimed in claim 1 stimulating electrode, it is characterized in that, described oxygen intake is arranged on the sidewall of bath, and described oxygen intake is consistent with the height of bottommost electrode.
8. the detachable temperature control bath of configuration field as claimed in claim 1 stimulating electrode, it is characterized in that, be provided with multiple electrode group in described bath, described multiple electrode groups are evenly arranged in bath.
9. the detachable temperature control bath of configuration field as claimed in claim 1 stimulating electrode, it is characterized in that, the diameter of described bath is 15mm, is highly 50mm, is respectively 19mm and 11mm bottom bath with the distance of described positive electrode, negative electrode vertical direction; Bottom described oxygen intake and bath, the distance of vertical direction is 11mm.
10. the using method of temperature control bath as described in claim 1-9 any one, is characterized in that, comprise step as follows:
1) bath is separated with full water jacket, opens bottom; Tested sample is hung over and links up with above, by tested sample successively through positive and negative electrode, be connected with tonotransducer;
2) bottom is connected with bath body; Described hook is alignd at vertical direction with electrode group;
3) in bath, fill body lotion, fill warm water between full water jacket and bath, temperature control perfusion entrance and the outlet of temperature control perfusion are by being connected with temperature control perfusion system; The temperature of described warm water is 36 DEG C ~ 37 DEG C;
4) by the temperature of temperature control perfusion system regulation and control bath environment;
5) electric stimulation operation is carried out, the change of test in vitro tissue shrink tension amplitude.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2878398Y (en) * | 2005-11-24 | 2007-03-14 | 上海中医药大学 | Determination device for unorganized tissue function |
| CN202027565U (en) * | 2011-03-31 | 2011-11-09 | 山东大学 | Multifunctional isolated organ experimental device |
| US20150209788A1 (en) * | 2014-01-24 | 2015-07-30 | Thermo Fisher Scientific (Asheville) Llc | Rack For Use With A Temperature Controlled Bath, And A Related Method |
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Non-Patent Citations (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
| CN112707017B (en) * | 2021-01-07 | 2022-02-18 | 首都医科大学 | Insulation can for separation heart experiments |
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