CN107894490A - A kind of in vitro formula cardiac perfusion device - Google Patents
A kind of in vitro formula cardiac perfusion device Download PDFInfo
- Publication number
- CN107894490A CN107894490A CN201710937847.6A CN201710937847A CN107894490A CN 107894490 A CN107894490 A CN 107894490A CN 201710937847 A CN201710937847 A CN 201710937847A CN 107894490 A CN107894490 A CN 107894490A
- Authority
- CN
- China
- Prior art keywords
- tube
- syringes
- perfusate
- perfusion device
- cardiac perfusion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
Abstract
The invention discloses a kind of in vitro formula cardiac perfusion device,Including base and support,The cradle top is fixed with water bath tube,The water bath tube is internally provided with K H liquid bottles and mixing gas cylinder,And the K H liquid bottle and gaseous mixture top of bottle are provided with charge door,The K H liquid bottle and gaseous mixture bottom of bottle portion are connected with ribbon mixer by lucite tube,The ribbon mixer is connected with peristaltic pump by glass tube,The peristaltic pump bottom is connected with perfusate sleeve pipe,And the glass tube between the peristaltic pump and perfusate sleeve pipe is connected with tipping tube,The tipping tube is connected with 20ml syringes,Limit screw is provided with the 20ml syringes,The perfusate sleeve bottom is provided with 20G liquid droping heads,The 20G liquid droping heads bottom is provided with perfusion ware,The perfusion ware is connected with 100ml syringes by rubber hose,And the 100ml syringes are arranged on the left end of support,The chassis left side is provided with transducer,The transducer is connected with rubber hose by glass tube.
Description
Technical field
The present invention relates to technical field of experiment equipment, specially a kind of in vitro formula cardiac perfusion device.
Background technology
Mammal isolated heart sustainer perfusion retrogradely method (Langendorff methods), is still cardiovascular research so far
An important technology method in field, its content also gradually expand to the transgene mouse model associated with cardiovascular system
On.Langendorff methods have constant pressure and the basic perfusion mode of two kinds of constant current.Two ways respectively has advantage and disadvantage and application,
Experimental provision is also different, and no matter the perfusion mode of constant pressure and constant current is required for glass tube to carry out constant temperature on mounting structure
The circulation of water, long pipeline when in use, then need to consider that its heat in flow process scatters and disappears, the longer heat of pipeline dissipates
That loses is faster, then needs the insulation path of permanent water-bath longer, and the change of temperature influences the accuracy of experimental data, while existing
The perfusion device of constant current and constant pressure lacks corresponding auxiliary liquid adding set, when experiment carries out auxiliary operation simultaneously, then needs
Stop original experimentation, influences the accuracy of experiment, and because the step of being operated in experiment is more, complicated pipeline connects
Connect and easily cause experiment accident.
The content of the invention
In order to overcome the shortcomings of prior art, the present invention provides a kind of in vitro formula cardiac perfusion device, realizes filling
The integrated design of device is flowed, has saved the installation number of glass tube, while to be generally held in constant water-bath insulating ring
Under border so that realize that data are more accurate.
The technical solution adopted for the present invention to solve the technical problems is：A kind of in vitro formula cardiac perfusion device, including bottom
Seat and support, the cradle top are fixed with water bath tube, and the water bath tube is internally provided with K-H liquid bottle and mixing gas cylinder, and institute
State K-H liquid bottle and gaseous mixture top of bottle is provided with charge door, the K-H liquid bottle and gaseous mixture bottom of bottle portion pass through lucite tube
Ribbon mixer is connected with, the ribbon mixer is connected with peristaltic pump by glass tube, and the peristaltic pump bottom is connected with filling
Flow liquid sleeve pipe, and the glass tube between the peristaltic pump and perfusate sleeve pipe is connected with tipping tube, the tipping tube is connected with
20ml syringes, limit screw are provided with the 20ml syringes, the water bath tube, peristaltic pump and perfusate jacket exterior are set
Stay-warm case is equipped with, the perfusate sleeve bottom is provided with 20G liquid droping heads, and the 20G liquid droping heads bottom is provided with perfusion ware, institute
State perfusion ware and 100ml syringes are connected with by rubber hose, and the 100ml syringes are arranged on the left end of support, it is described
Chassis left side is provided with transducer, and the transducer is connected with rubber hose by glass tube.
As a kind of technical scheme for having choosing of the present invention, the peristaltic pump is by being electrically connected with transducer.
As a kind of technical scheme for having choosing of the present invention, the perfusion ware surface is provided with stay-warm case.
As a kind of technical scheme for having choosing of the present invention, the K-H liquid bottle and mixing gas cylinder use pmma material, and
The oxygen and 5% carbon dioxide gas mixture that the mixing gas cylinder internal is 95%.
As a kind of technical scheme for having choosing of the present invention, the bath temperature in the water bath tube is not higher than 45 °.
As a kind of technical scheme for having choosing of the present invention, manual ball valve is provided with the charge door and tipping tube.
As a kind of technical scheme for having choosing of the present invention, the water bath tube, peristaltic pump and perfusate jacket exterior are provided with
Stay-warm case.
As a kind of technical scheme for having choosing of the present invention, the stay-warm case uses polyvinyl resin material.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention is connected by direct glass tube, and in entirety
Outside is provided with stay-warm case so as to be effectively incubated to overall, by the way that perfusate charging bottle is placed in water bath tube
Portion, it is easy to the mixing of raw material to preheat, so that follow-up is more fully mixed, heart capsule is adjusted by 100ml syringes
Volume size, by 20ml syringes to dosing in perfusate, the syringe connected by tipping tube 12 can be good at saving
Decoction.
Brief description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
In figure：1- bases；2- supports；3- transducers；4- stay-warm cases；5-K-H liquid bottles；6- mixing gas cylinders；7- water bath tubes；8-
Ribbon mixer；9- charge doors；10- peristaltic pumps；11-20ml syringes；12- tipping tubes；13- perfusate sleeve pipes；14-100ml
Syringe；15-20G liquid droping heads；16- perfusion wares；17- rubber hose；18- limit screws.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
The explanation of following embodiment is refer to the attached drawing, can be to the specific embodiment implemented to the example present invention.
The direction and position term that the present invention is previously mentioned, for example, " on ", " in ", " under ", "front", "rear", "left", "right", " interior ", "
Outside ", " side " etc., only it is direction and position with reference to annexed drawings.Therefore, the direction and position term used is to illustrate
And understand the present invention, and it is not used to the limitation present invention.
Embodiment：
As shown in figure 1, the invention provides a kind of in vitro formula cardiac perfusion device, including base 1 and support 2, the branch
The top of frame 2 is fixed with water bath tube 7, and the water bath tube 7 is internally provided with K-H liquid bottle 5 and mixing gas cylinder 6, and the K-H liquid bottle 5
Charge door 9 is provided with the top of mixing gas cylinder 6, the K-H liquid bottle 5 is connected with mixing gas cylinder 6 bottom by lucite tube
There is ribbon mixer 8, by the inside that K-H liquid bottle 5 and mixing gas cylinder 6 are placed on to water bath tube 7 so that the solution in bottle is gentle
The liveness well of holding, good mixing is then kept by the K-H solution and blender of ribbon mixer 8 before body remixes
Degree, avoid the degree of mixing problem of perfusate from influenceing the accuracy of experiment, the bottleneck of K-H liquid bottle 5 and mixing gas cylinder 6 is provided with hand
Dynamic ball valve is easy to charging and sealing in bottle；
The ribbon mixer 8 is connected with peristaltic pump 10 by glass tube, and the bottom of peristaltic pump 10 is connected with perfusate
Sleeve pipe 13, and the glass tube between the peristaltic pump 10 and perfusate sleeve pipe 13 is connected with tipping tube 12, the tipping tube 12 connects
20ml syringes 11 are connected to, limit screw 18, the water bath tube 7, peristaltic pump 13 and filling are provided with the 20ml syringes 11
Stay-warm case 4 is provided with outside flow liquid sleeve pipe 13, the stay-warm case 4 uses polyvinyl resin material, the bottom of perfusate sleeve pipe 13
Portion is provided with 20G liquid droping heads 15, and the bottom of 20G liquid droping heads 15 is provided with perfusion ware 16, and the perfusion ware 16 is soft by rubber
Pipe 17 is connected with 100ml syringes 14, and the 100ml syringes 14 are arranged on the left end of support 2, and the chassis left side is set
There is transducer 3, the transducer 3 is connected with rubber hose 17 by glass tube, is controlled by the turn of tidal stream of peristaltic tube 10, can incite somebody to action
The flow velocity of perfusion feeds back to transducer 3 in real time, and carries out adjustability control to flow velocity by transducer 3, wherein for water-bath
It is made up, is shelved in use steadily, the water bath shampoo maximum temperature of splendid attire can not surpass of high-strength lucite with the part of perfusion ware
45 DEG C are crossed, to prevent from damaging body lotion and perfusion device or cause leakage, the liquid in K-H liquid bottle 5 is transported to sample and connected by peristaltic pump
Depressed place is connect, heart perfusion, the volume size of heart capsule is adjusted by 100ml syringes, is added by 20ml syringes in perfusate
Medicine, the syringe connected by tipping tube 12 can be good at saving decoction, and device is mainly integrally provided with stay-warm case 4, so as to
During device integral experiment, the state of constant temperature can be kept, and the connection of directly glass tube reduces excessive pipeline
Circulation and heating.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.
Claims (8)
1. a kind of in vitro formula cardiac perfusion device, including base (1) and support (2), it is characterised in that：At the top of the support (2)
Water bath tube (7) is fixed with, the water bath tube (7) is internally provided with K-H liquid bottle (5) and mixing gas cylinder (6), and the K-H liquid bottle
(5) charge door (9) and at the top of mixing gas cylinder (6) is provided with, the K-H liquid bottle (5) and mixing gas cylinder (6) bottom pass through organic
Glass tube is connected with ribbon mixer (8), and the ribbon mixer (8) is connected with peristaltic pump (10) by glass tube, described compacted
Dynamic pump (10) bottom is connected with perfusate sleeve pipe (13), and the glass tube between the peristaltic pump (10) and perfusate sleeve pipe (13)
Tipping tube (12) is connected with, the tipping tube (12) is connected with 20m l syringes (11), set on the 20ml syringes (11)
There is limit screw (18), perfusate sleeve pipe (13) bottom is provided with 20G liquid droping heads (15), 20G liquid droping heads (15) bottom
Portion is provided with perfusion ware (16), and the perfusion ware (16) is connected with 100ml syringes (14) by rubber hose (17), and described
100ml syringes (14) are arranged on the left end of support (2), and the chassis left side is provided with transducer (3), the transducer (3)
Rubber hose (17) is connected with by glass tube.
A kind of 2. in vitro formula cardiac perfusion device according to claim 1, it is characterised in that：The peristaltic pump (10) passes through
It is electrically connected with transducer (3).
A kind of 3. in vitro formula cardiac perfusion device according to claim 1, it is characterised in that：Perfusion ware (16) surface
It is provided with stay-warm case.
A kind of 4. in vitro formula cardiac perfusion device according to claim 1, it is characterised in that：The K-H liquid bottle (5) and mixed
Close gas cylinder (6) and use pmma material, and mixed inside the mixing gas cylinder (6) for 95% oxygen and 5% carbon dioxide
Close gas.
A kind of 5. in vitro formula cardiac perfusion device according to claim 4, it is characterised in that：In the water bath tube (7)
Bath temperature is not higher than 45 °.
A kind of 6. in vitro formula cardiac perfusion device according to claim 1, it is characterised in that：The charge door (9) and incline
Inclined tube is provided with manual ball valve on (12).
A kind of 7. in vitro formula cardiac perfusion device according to claim 1, it is characterised in that：The water bath tube (7), wriggle
Stay-warm case (4) is provided with outside pump (13) and perfusate sleeve pipe (13).
A kind of 8. in vitro formula cardiac perfusion device according to claim 7, it is characterised in that：The stay-warm case (4) uses
Polyvinyl resin material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710937847.6A CN107894490A (en) | 2017-10-11 | 2017-10-11 | A kind of in vitro formula cardiac perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710937847.6A CN107894490A (en) | 2017-10-11 | 2017-10-11 | A kind of in vitro formula cardiac perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107894490A true CN107894490A (en) | 2018-04-10 |
Family
ID=61803258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710937847.6A Withdrawn CN107894490A (en) | 2017-10-11 | 2017-10-11 | A kind of in vitro formula cardiac perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107894490A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108742875A (en) * | 2018-06-08 | 2018-11-06 | 新乡医学院 | A kind of high-efficiency in-vitro cardiac perfusion device |
| CN112471990A (en) * | 2020-11-10 | 2021-03-12 | 苏州高之仙自动化科技有限公司 | Intelligent stone nursing robot liquid dropping system assembly |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
-
2017
- 2017-10-11 CN CN201710937847.6A patent/CN107894490A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108742875A (en) * | 2018-06-08 | 2018-11-06 | 新乡医学院 | A kind of high-efficiency in-vitro cardiac perfusion device |
| CN112471990A (en) * | 2020-11-10 | 2021-03-12 | 苏州高之仙自动化科技有限公司 | Intelligent stone nursing robot liquid dropping system assembly |
| CN112471990B (en) * | 2020-11-10 | 2022-02-18 | 苏州高之仙自动化科技有限公司 | Intelligent stone nursing robot liquid dropping system assembly |
| CN112707017A (en) * | 2021-01-07 | 2021-04-27 | 首都医科大学 | Insulation can for separation heart experiments |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180410 |