CN204727898U - A kind of cell culture bags - Google Patents
A kind of cell culture bags Download PDFInfo
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- CN204727898U CN204727898U CN201520383329.0U CN201520383329U CN204727898U CN 204727898 U CN204727898 U CN 204727898U CN 201520383329 U CN201520383329 U CN 201520383329U CN 204727898 U CN204727898 U CN 204727898U
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- cylindrical shell
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Abstract
The utility model relates to a kind of cell culture bags, comprise bag, catheter and cylindrical shell, described bag is provided with liquid outlet and thief hole, one end of described cylindrical shell is provided with the first opening, the other end of described cylindrical shell is provided with the second opening, described liquid outlet is connected by catheter with the first opening, and the internal surface of described bag is provided with fold.Cell culture bags of the present utility model have reduce cell contamination probability, reduce the wasting of resources and be beneficial to cell in bag and obtain better active advantage.
Description
Technical field
The utility model relates to cell culture apparatus, particularly a kind of cell culture bags.
Background technology
Cellular immunotherapy is that a very swift and violent technology is developed in medical oncology field in recent years, the 4th kind of oncotherapy technology after operation, chemotherapy, radiotherapy three great tradition therapy, the tumour cell that traditional remedies is residual can be killed, prevent tumor recurrence and transfer, reach the object that tumour is even cured in control.Cellular immunotherapy is by gathering autologous patient peripheral blood, isolating immune cells, and through vitro culture induction, a large amount of amplifications have the immunocyte of target killing function, and then feed back to human body with killing off tumor cells.
At present, immunocyte cultivates general employing 1-2L cell culture bags, the lid that nutrient solution needs to open catheter is added in cell amplification culturing process, connect the empty cylinder of the syringe removing piston, then be positioned on test-tube stand, inject nutrient solution, abandon the empty cylinder of syringe after having operated, screw back the lid of catheter.In the process of cell cultures, need the operation repeatedly adding nutrient solution, the drain mouth of pipe also needs repeatedly nozzle empty with syringe to be connected, and operation owes convenient, easily increases the probability of cell contamination, and each operation all needs to use new syringe, causes the wasting of resources.
Utility model content
Technical problem to be solved in the utility model is: provide a kind of easy to operate and reduce the cell culture bags of cell contamination.
In order to solve the problems of the technologies described above, the technical solution adopted in the utility model is:
A kind of cell culture bags, comprise bag, catheter and cylindrical shell, described bag is provided with liquid outlet and thief hole, one end of described cylindrical shell is provided with the first opening, the other end of described cylindrical shell is provided with the second opening, described liquid outlet is connected by catheter with the first opening, and the internal surface of described bag is provided with fold.
Further, described cylindrical shell comprises the first cylindrical shell and the second cylindrical shell that connect successively, described first opening is arranged at the first cylindrical shell one end away from the second cylindrical shell, described second opening is arranged at the second cylindrical shell one end away from the first cylindrical shell, described first cylindrical shell is frustum, and one end that the section area of the first cylindrical shell of described frustum is little arranges the first opening.
Further, the two ends of described catheter are respectively equipped with cylindric internal thread, and described first opening and liquid outlet are respectively equipped with coniform outside screw.
Further, described second opening is provided with sealing cover, and described sealing cover is screw-cap.
Further, described thief hole is provided with sealing member.
Further, described cylindrical shell is provided with mounting block near the position of the second opening.
Further, described cylindrical shell is provided with scale along the first opening to the second opening direction.
Further, described cylindrical shell and catheter are detachably arranged.
Further, described fold has depressed part and lug boss.
The beneficial effects of the utility model are:
(1) catheter connector drum, in cell cultivation process during adding liquid, open the second opening, nutrient solution is injected toward cylindrical shell, thus to avoid in cell cultivation process repeatedly plugging into the empty cylinder of syringe at the drain mouth of pipe, reduce the probability of cell contamination, also reduce the usage quantity of syringe in cell cultivation process simultaneously, reduce the wasting of resources; Only need dismantle cylindrical shell when carrying out cell harvesting before clinical reinfusion cell, pour cell suspension into collection tube by liquid outlet along catheter, easy and simple to handle, the probability of cell contamination can be reduced;
(2) arrange fold at the internal surface of bag, increased the surface-area of bag by fold, the cell be beneficial in bag obtains better growth and activity.
Accompanying drawing explanation
Fig. 1 is the one-piece construction figure of the cell culture bags of the utility model embodiment.
Label declaration:
1, bag; 12, liquid outlet; 13, thief hole; 14, fold; 15, depressed part; 16, lug boss; 2, catheter; 3, cylindrical shell; 31, the first opening; 32, the second opening; 33, the first cylindrical shell; 34, the second cylindrical shell; 35, sealing cover; 36, mounting block; 37, scale.
Embodiment
By describing technology contents of the present utility model in detail, realized object and effect, accompanying drawing is coordinated to be explained below in conjunction with embodiment.
The design of the utility model most critical is: by catheter connector drum, thus avoids in cell cultivation process because catheter is repeatedly plugged into the empty cylinder of syringe and the cell contamination problem brought.
Please refer to Fig. 1, a kind of cell culture bags of the present utility model, comprise bag 1, catheter 2 and cylindrical shell 3, described bag 1 is provided with liquid outlet 12 and thief hole 13, one end of described cylindrical shell 3 is provided with the first opening 31, the other end of described cylindrical shell 3 is provided with the second opening 32, and described liquid outlet 12 is connected by catheter 2 with the first opening 31, and the internal surface of described bag 1 is provided with fold 14.
From foregoing description, the beneficial effects of the utility model are:
(1) by catheter connector drum, thus avoid catheter in cell cultivation process repeatedly to plug into the empty cylinder of syringe, reduce the probability of cell contamination, also reduce the usage quantity of syringe in cell cultivation process simultaneously, reduce the wasting of resources;
(2) arrange fold at the internal surface of bag, increased the surface-area of bag by fold, the cell be beneficial in bag obtains better growth and activity.
Further, described cylindrical shell 3 comprises the first cylindrical shell 33 and the second cylindrical shell 34 connected successively, described first opening 31 is arranged at the first cylindrical shell 33 one end away from the second cylindrical shell 34, described second opening 32 is arranged at the second cylindrical shell 34 one end away from the first cylindrical shell 33, described first cylindrical shell 33 is frustum, and one end that the section area of the first cylindrical shell 33 of described frustum is little arranges the first opening 31.
Seen from the above description, the frustum design of the first cylindrical shell, is conducive to reducing the residual quantity of liquid in cylindrical shell.
Further, the two ends of described catheter 2 are respectively equipped with cylindric internal thread, and described first opening 31 and liquid outlet 12 are respectively equipped with coniform outside screw.
Seen from the above description, cylindric internal thread and the coniform externally threaded design be fastenedly connected can strengthen intensity and the tightness of interface, liquid not easy to leak in adding liquid process.
Further, described second opening 32 is provided with sealing cover 35, and described sealing cover 35 is screw-cap.
Seen from the above description, the setting of sealing cover has uncaps conveniently, is convenient to the carrying out of adding nutrient solution operation.
Further, described thief hole 13 is provided with sealing member.
Seen from the above description, thief hole is provided with sealing member, is sealed by thief hole.
Further, described cylindrical shell 3 is provided with mounting block 36 near the position of the second opening 32.
Seen from the above description, mounting block arranges and can be stuck on test-tube stand by cylindrical shell easily, adds nutrient solution by cylindrical shell directly to bag.
Further, described cylindrical shell 3 is provided with scale 37 along the first opening 31 to the second opening direction 32.
Seen from the above description, cylindrical shell is arranged the scale personnel that are convenient to operation and quantitatively add blood plasma needed for cell cultures and cytokine.
Further, described cylindrical shell 3 is detachably arranged with catheter 2.
Seen from the above description, the design of catheter connector drum simplifies the operation steps of adding nutrient solution, only need cylindrical shell be stuck on test-tube stand by mounting block during each interpolation nutrient solution, open the sealing cover on cylindrical shell, directly nutrient solution is added by cylindrical shell, end of operation bonnet top cover labyrinth, does not need all to connect syringe empty cylinder during each adding liquid.And catheter and cylindrical shell detachably, only need be dismantled cylindrical shell when carrying out cell harvesting before clinical reinfusion cell, pour cell suspension into collection tube by liquid outlet along catheter, easy and simple to handle, can reduce the probability of cell contamination.
Further, described fold 14 has depressed part 15 and lug boss 16.
Seen from the above description, the depressed part of fold and the design of lug boss can increase gas exchange area further, are conducive to cell and obtain better growth conditions and activity.
Please refer to Fig. 1, embodiment one of the present utility model is:
A kind of cell culture bags of the present embodiment, comprise bag 1, catheter 2 and cylindrical shell 3, described bag 1 is provided with liquid outlet 12 and thief hole 13, one end of described cylindrical shell 3 is provided with the first opening 31, the other end of described cylindrical shell 3 is provided with the second opening 32, described liquid outlet 12 is connected by catheter 2 with the first opening 31, and the internal surface of described bag 1 is provided with fold 14.Described cylindrical shell 3 comprises the first cylindrical shell 33 and the second cylindrical shell 34 connected successively, described first opening 31 is arranged at the first cylindrical shell 33 one end away from the second cylindrical shell 34, described second opening 32 is arranged at the second cylindrical shell 34 one end away from the first cylindrical shell 33, described first cylindrical shell 33 is frustum, and one end that the section area of the first cylindrical shell 33 of described frustum is little arranges the first opening 31.The two ends of described catheter 2 are respectively equipped with cylindric internal thread, and described first opening 31 and liquid outlet 12 are respectively equipped with coniform outside screw.Described second opening 32 is provided with sealing cover 35, and described sealing cover 35 is screw-cap.Described thief hole 13 is provided with sealing member.Described cylindrical shell 3 is provided with mounting block 36 near the position of the second opening 32.Described cylindrical shell 3 is provided with scale 37 along the first opening 31 to the second opening direction 32.Described cylindrical shell 3 is detachably arranged with catheter 2.Described fold 14 has depressed part 15 and lug boss 16.
In sum, the cell culture bags that the utility model provides have reduce cell contamination probability, reduce the wasting of resources and be beneficial to cell in bag and obtain better active advantage.
The foregoing is only embodiment of the present utility model; not thereby the scope of the claims of the present utility model is limited; every equivalents utilizing the utility model specification sheets and accompanying drawing content to do; or be directly or indirectly used in relevant technical field, be all in like manner included in scope of patent protection of the present utility model.
Claims (9)
1. a cell culture bags, it is characterized in that, comprise bag, catheter and cylindrical shell, described bag is provided with liquid outlet and thief hole, one end of described cylindrical shell is provided with the first opening, the other end of described cylindrical shell is provided with the second opening, and described liquid outlet is connected by catheter with the first opening, and the internal surface of described bag is provided with fold.
2. cell culture bags according to claim 1, it is characterized in that, described cylindrical shell comprises the first cylindrical shell and the second cylindrical shell that connect successively, described first opening is arranged at the first cylindrical shell one end away from the second cylindrical shell, described second opening is arranged at the second cylindrical shell one end away from the first cylindrical shell, described first cylindrical shell is frustum, and one end that the section area of the first cylindrical shell of described frustum is little arranges the first opening.
3. cell culture bags according to claim 1, is characterized in that, the two ends of described catheter are respectively equipped with cylindric internal thread, and described first opening and liquid outlet are respectively equipped with coniform outside screw.
4. cell culture bags according to claim 1, is characterized in that, described second opening is provided with sealing cover, and described sealing cover is screw-cap.
5. cell culture bags according to claim 1, is characterized in that, described thief hole is provided with sealing member.
6. cell culture bags according to claim 1, is characterized in that, described cylindrical shell is provided with mounting block near the position of the second opening.
7. cell culture bags according to claim 1, is characterized in that, described cylindrical shell is provided with scale along the first opening to the second opening direction.
8. cell culture bags according to claim 1, is characterized in that, described cylindrical shell and catheter are detachably arranged.
9. cell culture bags according to claim 1, is characterized in that, described fold has depressed part and lug boss.
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CN201520383329.0U CN204727898U (en) | 2015-06-04 | 2015-06-04 | A kind of cell culture bags |
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CN201520383329.0U CN204727898U (en) | 2015-06-04 | 2015-06-04 | A kind of cell culture bags |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107586717A (en) * | 2017-10-31 | 2018-01-16 | 河南省肿瘤医院 | A kind of attachment means of cell culture bags and culture jar |
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2015
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107586717A (en) * | 2017-10-31 | 2018-01-16 | 河南省肿瘤医院 | A kind of attachment means of cell culture bags and culture jar |
CN107586717B (en) * | 2017-10-31 | 2024-03-26 | 河南省肿瘤医院 | Connecting device for cell culture bag and culture solution bottle |
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