CN112675088B - Composition with relieving and repairing effects and preparation method and application thereof - Google Patents
Composition with relieving and repairing effects and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a composition with a soothing and repairing effect, and a preparation method and application thereof. The composition with soothing and repairing effects comprises Tinospora cordifolia extract, trehalose and hydrolyzed hazelnut protein. The composition with the effect of relieving and repairing provided by the invention achieves the purpose of relieving and repairing the skin by preventing, protecting and repairing the skin barrier damage caused by various factors through the synergistic cooperation of the components, and has a better relieving and repairing effect.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composition with a soothing and repairing effect, and a preparation method and application thereof.
Background
The skin is the first barrier of the human body and has important physiological significance in maintaining the stability of the internal environment of the body and resisting harmful factors of the external environment. Some skin diseases such as allergy, dry and itchy hair, etc. are easy to repeatedly attack and difficult to find out specific reasons, but they have a common point- "skin barrier is damaged". The damaged skin barrier refers to the phenomenon that arrangement of keratinocytes is disordered and loose, namely, the skin loses normal protection function and cannot resist external stimulation, so that the skin is easy to become dry and deficient due to poor water locking capacity, the skin is poor or allergic due to harmful substance permeation, dermatitis and other various skin diseases are recurrent and the like. Therefore, the protection and repair of the skin barrier are of great significance to the maintenance of skin health.
Normally, the skin barrier has a certain resistance to the environment, but the skin barrier of many consumers is damaged to different degrees due to long-term wrong skin care habits, bad eating habits, environmental stimuli and other external factors. With the improvement of the scientific and technical level and the wide popularization of the concept of barrier repair and skin care, more and more consumers pay attention to the skin barrier. The skin barrier is composed of a sebaceous membrane, a stratum corneum cell, an extracellular matrix and the like, the function of the skin barrier is influenced by the structure of the skin barrier, and other epidermal cells play an important role in the skin barrier. Patents CN109200010A, CN111631987A disclose compositions that can repair skin barriers, but they do not mention specific repair mechanisms and angles of repair.
Therefore, there is a need for a composition and cosmetic product that can collectively promote barrier repair from multiple aspects.
Disclosure of Invention
The invention aims to overcome the defects or shortcomings of skin barrier repair in the prior art, and provides a composition with a soothing and repairing effect. The composition provided by the invention not only solves the problems of skin inflammation and damaged keratinocytes unilaterally, but also combines a plurality of nodes to promote the repair of skin barriers together, thereby fundamentally recovering the health of skin and having better repair effect.
Another object of the present invention is to provide a method for preparing the above composition having soothing and repairing effects.
Another object of the present invention is to provide the use of the above-mentioned composition with soothing and repairing action for the preparation of cosmetics or toiletries.
Another object of the present invention is to provide a bathing article.
In order to realize the purpose of the invention, the invention adopts the following technical scheme:
a composition with a soothing and repairing effect comprises the following components in parts by weight:
0.01 to 6.0 parts of tinospora cordifolia extract,
0.01 to 6.0 portions of trehalose,
0.01 to 6.0 parts of hydrolyzed hazelnut protein.
Tinospora cordifolia extract: the extraction part is selected from root and stem of Tinospora cordifolia, the chemical components mainly comprise Tinospora leaf picrocarpus, palmatine hydrochloride, jateorhizine and the like, the extract has obvious relieving effect on inflammation and allergic symptoms, has good treatment effect on skin problems caused by scabies and mite infection, and has good relieving effect when being added into cosmetics. The Tinospora cordifolia extract has certain absorption capacity on ultraviolet rays and a chelating effect on metal ions, so that the Tinospora cordifolia extract can reduce the accumulation of ROS in keratinocytes, inhibit the activity of various MMPS enzymes, effectively protect skin extracellular matrix from being disintegrated, and further enhance the skin barrier. It also has the ability to promote epidermal cell growth, which is related to its ability to have relevant immunomodulation and the ability to activate cells.
The Tinospora cordifolia extract can be obtained by ethanol reflux extraction, the extraction solvent is 75% ethanol solution, and the extraction temperature is 75-80 ℃; it is also commercially available.
Trehalose: trehalose is a disaccharide consisting of two alpha-D-glucopyranosyl groups, usually contains 2 crystal waters, is a natural saccharide, belongs to a small molecule humectant, and can be used as a skin penetrant to increase the absorption of nutrients into the skin. Lipid is closely related to the barrier function of skin, when lipid is deficient due to various reasons, the water barrier function of the skin is weakened, trehalose can promote the absorption of lipid substances and can also inhibit the oxidative decomposition of fatty acid to the maximum extent, and the skin hydration function of cells is enhanced to a certain extent, so that the skin dryness and desquamation caused by the increase of the moisture loss of the epidermis can be effectively relieved, and the moisturizing effect is good. Trehalose also has good affinity with membrane protein, can be combined with the membrane protein on the surface of a cell membrane to form glycoprotein to protect protein denaturation damage on the surface layer of skin, and OH-of the trehalose and the head of phospholipid molecule acyl choline form hydrogen bond connection, so that double-layer phospholipid is not easy to hydrolyze and separate, thereby playing a good protection role on the cell membrane. The protection effect of trehalose on keratinocytes is also embodied in the protection of DNA integrity, the trehalose can enter cells through cell membranes to effectively protect the DNA integrity, has a certain resistance effect on radiation damage, can inhibit UV irradiation to induce active oxygen free radicals to activate MMPS, and can reduce the degradation of almost all molecules in skin extracellular matrix. The trehalose can prolong the activity of epidermal growth factor at normal temperature, and can effectively resist skin photoaging. In addition, trehalose has a good effect in improving the tolerance of the skin to high pH and the anti-allergic performance of other irritants.
Trehalose is commercially available.
Hydrolysis of hazelnut protein: is a bioactive peptide derived from hazelnut protein, and contains 3-20 amino acid residues. The hazelnut protein contains 8 amino acids necessary for human body, especially contains arginine with high content, which can promote muscle protein synthesis and enhance organism immunity, and has important effect in physiological process of cell division, wound recovery, etc., so the hydrolysate has better repairing effect on skin. Arginine and lysine contained in the hydrolysate of hazelnut protein can react with unsaturated fatty acid to generate salt, so that the oxidation reaction of the fatty acid can be inhibited. It also has the function of chelating metal ions, has good inhibition effect on the activation of matrix metalloproteinase, effectively reduces the degradation of extracellular matrix protein and other bioactive molecules, and greatly enhances the skin barrier function. It can also effectively resist aging, has strong capability of capturing and removing free radicals which is 138 times of VE, and can play a role in resisting aging by regulating and controlling photoaging signal channels. Based on the hydration of intramolecular hydrogen bonds and ionic groups, the bioactive peptide has better water retention property, can keep the moisture of the stratum corneum of the skin to be more than 10 percent, has certain hydrophobicity on the other hand, and can form a hydrophobic film on the surface of the skin. The water retention property and the film forming property play a role simultaneously, the skin is helped to lock water, and a good moisturizing effect is achieved, so that the barrier function of the skin is greatly enhanced. The skin soothing effect of the hydrolyzed hazelnut protein is represented by an immunoregulation effect and a bactericidal effect, the hydrolyzed hazelnut protein can regulate synthesis and release of inflammatory factors and proliferation and activity of immune cells so as to relieve inflammatory reaction of the skin, and the hydrolyzed hazelnut protein can act on cell membranes of bacteria and mould on the surface of the skin so as to change the permeability of the bacteria and the mould until the bacteria and the mould are cracked and killed, so that the skin soothing effect is improved to a certain extent.
The hydrolyzed hazelnut protein is commercially available.
The invention achieves the purpose of relieving and repairing the skin by preventing, protecting and repairing the skin barrier damage caused by various factors, and the three active ingredients are cooperated to play a role in the process, thereby fundamentally improving the skin barrier function through various ways. The method specifically comprises the following steps: the Tinospora cordifolia and the hydrolyzed hazelnut protein are utilized to promote the proliferation of epidermal cells, the skin water retention and moisture retention capacity is enhanced by compounding trehalose, the anti-allergic and anti-inflammatory effects are achieved, and the other three can directly or indirectly inhibit the activity of MMPS enzyme, so that the extracellular matrix is effectively protected from being disintegrated, and the damage of the skin barrier is effectively protected.
Through the synergistic effect of the three components, the composition with the soothing and repairing effect provided by the invention enhances the skin cell barrier from multiple dimensions, so that the soothing and repairing of the skin is realized. Specifically, the following effects can be achieved: reducing TEWL (water loss of skin through epidermis), maintaining the water content of skin surface above 10%, relieving dry itching and redness, and recovering skin health state.
Preferably, the composition with the effect of relieving and repairing comprises the following components in parts by weight:
2.0 to 4.0 parts of Tinospora cordifolia extract,
2.0 to 4.0 portions of trehalose,
2.0-4.0 parts of hydrolyzed hazelnut protein.
More preferably, the composition with the effect of relieving and repairing comprises the following components in parts by weight:
2 parts of Tinospora cordifolia extract,
4 parts of trehalose, namely 4 parts of trehalose,
3 parts of hydrolyzed hazelnut protein.
The preparation method of the composition with the relieving and repairing effects comprises the following steps: mixing the Tinospora cordifolia extract, trehalose and hydrolyzed hazelnut protein to obtain the composition with the effects of relieving and repairing.
The use of the above-mentioned composition with soothing and repairing action for the preparation of cosmetics or toiletries is also within the scope of protection of the present invention.
Preferably, the composition with the effect of relieving and repairing is added into cosmetics or washing care products in an amount of 5-60%.
A bath product comprises the following components in parts by mass:
5 to 30 percent of the composition with the relieving and repairing functions,
10 to 16 percent of foaming agent,
2 to 5 percent of thickening stabilizer,
the balance of other auxiliary agents.
Preferably, the bath product comprises the following components in parts by mass:
10 percent of composition with the function of relieving and repairing,
13.0 percent of foaming agent,
3.0 percent of thickening stabilizing agent,
the balance of other auxiliary agents.
Preferably, the foaming agent is one or more of sodium laureth sulfate, decyl glucoside, sodium methylacyl taurate, sodium lauroyl sarcosinate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, lauramidopropyl betaine or sodium lauroamphoacetate.
Preferably, the thickening stabilizer is one or more of sodium chloride, cocamide DEA, cocamide methyl MEA, PEG-120 methyl glucose triisostearate or acrylic acid (ester) copolymer.
Preferably, the other auxiliary agents are one or more of water, propylene glycol, glycerol, butanediol, phenoxyethanol, benzyl alcohol, sodium benzoate, EDTA disodium, citric acid or sodium citrate.
Compared with the prior art, the invention has the following beneficial effects:
the composition with the soothing and repairing effects achieves the aim of soothing and repairing the skin by preventing, protecting and repairing the skin barrier damage caused by various factors through the synergistic cooperation of the components, and has a better soothing and repairing effect.
Detailed Description
The invention is further illustrated by the following examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Experimental procedures without specific conditions noted in the examples below, generally according to conditions conventional in the art or as suggested by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, those commercially available from the conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
The raw materials selected in the examples and comparative examples of the invention are as follows:
tinospora cordifolia extract: the tinospora cordifolia extract is obtained by ethanol reflux extraction; taking dried tubers or roots of Tinospora cordifolia, crushing, adding an ethanol water solution, heating, refluxing, extracting, filtering, concentrating under reduced pressure to obtain a crude extract, purifying the crude extract by adopting macroporous resin, concentrating an eluent into an extract or a solid, adding butanediol, dissolving and filtering, and obtaining a filtrate, namely a Tinospora cordifolia extracting solution;
trehalose: the raw materials sold in the market are not limited by manufacturers, and the purity is more than 98%;
hydrolysis of hazelnut protein: the raw material is sold in the market, and the main component of the raw material is polypeptide with the molecular weight of 300-3000 daltons.
Examples 1 to 7
This example provides a range of bath products having the formulation shown in table 1.
Table 1 formulations (mass fraction%) of bath products provided in examples 1 to 3
Table 2 formula (mass fraction%) of bath products provided in examples 1, 4 to 7
The composition 1# with the effect of relieving and repairing is prepared by the following method: mixing the Tinospora cordifolia extract 2 parts, trehalose 4 parts and hydrolyzed hazelnut protein 3 parts.
The composition 2# with the effect of relieving and repairing is prepared by the following method: mixing 2 parts of Tinospora cordifolia extract, 2 parts of trehalose and 4 parts of hydrolyzed hazelnut protein.
The composition 3# with the effect of relieving and repairing is prepared by the following method: mixing the extract of Tinospora cordifolia 4 parts, trehalose 4 parts and hydrolyzed hazelnut protein 2 parts.
Composition No. 4 with soothing and repairing effects is prepared by the following method: mixing Tinospora cordifolia extract 0.01 parts, trehalose 6 parts and hydrolyzed hazelnut protein 0.01 parts.
The composition 5# with the effect of relieving and repairing is prepared by the following method: mixing the Tinospora cordifolia extract 6 parts, trehalose 0.01 part and hydrolyzed hazelnut protein 6 parts.
Compositions 1 to 3 are similar to the preparation of composition 1# with soothing and repairing action, with the difference that: the composition 1# central leaf swertia mileensis extract, trehalose and hydrolyzed hazelnut protein with the soothing and repairing effects are sequentially replaced by equivalent deionized water, and the composition 1-3 is obtained.
Comparative examples 1 to 4
This comparative example provides a series of bath compositions formulated as in table 3.
Table 3 cleansing compositions provided in comparative examples 1 to 3 (mass%)
In each of the examples and comparative examples, the surfactant was composed of 11 parts of sodium laureth sulfate, 1 part of sodium lauroyl sarcosinate, and 1 part of cocamidopropyl betaine; the thickening stabilizer consists of 1.5 parts of coconut oil amide methyl MEA and 1.5 parts of sodium chloride; other auxiliary agents comprise 1.0 part of propylene glycol, 0.4 part of benzyl alcohol, 0.4 part of sodium benzoate, 0.02 part of citric acid and the balance of water.
Comparative example 4 is a cool and refreshing bath lotion with certain color and skin tendering.
Performance testing
1. Compositions 1# to 5# and compositions 1 to 3# with soothing and healing action were tested according to the following test methods.
1. Moisture percutaneous loss (TEWL) determination
The magnitude of the TEWL value is an important sign for evaluating the strength of the skin barrier function.
Selecting 16 subjects with 20-40 years old dry skin, marking 3X 3cm on the inner side of the front wall of the two hands of the subject 2 In the test area, the same arm can mark a plurality of areas, and the areas are spaced by 1cm. Both test samples and blank controls were randomly distributed on the left and right arms. The measurement of the test area and the control area was performed using a capacitance skin meter. Each area was measured in parallel 15 times, with the blank paper of each test area being measured and then 2.0. + -. 0.1mg of sample/cm 2 The amounts of the components were uniformly applied to the test area. The skin moisture content of the test area and the blank area (measured at this time for verification) was measured at 1, 2, and 4 hours for each application. Any product (cosmetic or external medicine) cannot be used for 2-3 days before the tested part. The temperature of the test environment is controlled at 25 +/-1 ℃, the relative humidity is 40 +/-5%, purified water at about 36 ℃ is used for wiping the two arms of the testee before the test, and the testee is allowed to sit still for 30min in the test environment for testing. And respectively measuring the TEWL value of each time interval according to the experimental design, and calculating the reduction amount of the skin water dispersion amount of each time point. The greater the reduction in skin moisture loss, the better the skin barrier repair, the results are shown in Table 4.
Compositions 1# to 5# and 1 x to 3# were each diluted with distilled water to a mass fraction of 10% of each composition to be used as test samples.
Calculating the formula: reduction in skin moisture loss amount = [ (T) 0 -T 1 )/T 0 ]X 100% where T 1 Corresponding TEWL for each time point subject group, where T 0 TEWLs corresponding to the blank control groups for each time point.
TABLE 4 reduction of skin moisture loss for compositions # 1-5 # and compositions # 1-3%
As can be seen from Table 4, the compositions of the above groups all reduced the amount of water dispersed in the skin over a certain period of time after they acted on the skin, and the effect of reducing composition No. 1 was most significant, and the amount of water dispersed in the skin of the group was reduced to 21.66% at 30min and to 19.86% at 120 min. The composition has a good effect of repairing skin barriers, and can effectively improve the dryness of the skin. Comparing composition 1# with compositions 1-3, it is clear that the skin-repairing effect of the composition is greatly reduced if one of the central leaf of the composition with soothing and repairing effects, the trehalose or the hydrolyzed hazelnut protein is absent.
2. Functional evaluation of the effect of the soothing sensitive stimulus:
24 volunteers with sensitive skin are selected, wherein the age is 20-35 years old, and the volunteers are half of male and female. The volunteers could not use any allergy-relieving product, such as cosmetics, external medicines or internal health products, 30 days before the start of the test. Before the test, the test piece is placed in a constant temperature and humidity room with the temperature of 22 ℃ and the humidity of 50% for standing for 30min, and in the test, a half face test is carried out according to a random table, wherein one side is a composition No. 1-5, and the other side is a composition No. 1-3. Selecting a nasolabial sulcus part of a subject to test, dripping 50ul of lactic acid solution on a single-layer filter paper with the diameter of 0.8cm, respectively inquiring the subject for stabbing pain feelings at 3min, 4min and 5min, grading according to a 4-point method (0 is no stabbing pain feeling, 1 is mild stabbing pain, 2 is moderate stabbing pain, 3 is severe stabbing pain, and the value of the adjacent stabbing pain interval can be taken according to actual conditions), and recording the grading of a volunteer as the lactic acid stimulation grading (D0) of the side. After the test, the volunteers needed to use the composition 1# -5# for one side face and 1-3 # for one side face. Cross-contamination was prohibited and samples were used 1 time per day as required and were asked to return on day 14 (D14) and day 28 (D28) of the test. The lighting conditions at each point in time should be consistent with the curtains closed, proper lighting. The subject was lying in bed. The angle of bed tilt should be consistent at each point in time. The position of the bed must be noted on the ground to ensure consistency of the positions of D14 and D28. The tests of the same volunteer were performed by the same measuring staff. The scores were recorded and the rate of change was calculated.
The calculation method comprises the following steps: test results rate of change = (DO-Dd)/DOX 100%, where DO is lactate stimulation score before use of the sample and Dd is lactate stimulation score measured at each return visit time.
The test results are shown in Table 5.
TABLE 5 compositions # 1 to # 5 and compositions # 1 to # 3 lactic acid stimulation scores
The lactic acid stimulation score results of table 5 clearly show the differences between compositions # 1-5 # and compositions # 1-3. Because the compounding of the tinospora cordifolia extract, the trehalose and the hydrolyzed hazelnut protein can effectively relieve the stimulation of lactic acid, if any one of the substances is lacked, the relieving effect is greatly reduced. The results of the tests comparing D14 and D28 show that the anti-irritation capability of the skin can be enhanced by using the composition for a long time. The composition No. 1-5 comparison shows that the composition No. 1 has the best relieving effect, which shows that the composition with the relieving and repairing effects has the more prominent relieving effect when the content of the extract of the tinospora cordifolia is 2.0%, the content of the trehalose is 4.0%, and the content of the hydrolyzed hazelnut protein is 3.0%.
3. Measurement of cell proliferation Effect
MTT staining is a method for detecting the survival and growth of cells by the principle that succinate dehydrogenase in mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-violet crystalline formazan and deposit it in cells, while dead cells do not have this function. Dimethyl sulfoxide (DMS 0) can dissolve formazan in cells, and its light absorption value is measured by enzyme linked immunosorbent detector at 540 or 720nm wavelength, which can indirectly reflect the number of living cells. Within a certain range of cell number, MTT crystals are formed in an amount proportional to the cell number.
The experimental method comprises the following steps:
(1) fibroblast (keratinocyte) culture: cultured fibroblasts (keratinocytes) were treated with Typsin-EDTA and collected, suspended in DMEM, counted using a hemocytometer, and diluted to a cell concentration of 5X 104cells/ml for use. The prepared cell suspension was aliquoted into 96well dish and cultured in an inoculum size of 100ul. Incubated at 37 ℃ for 24 hours in an incubator containing 5% C02.
(2) Adding samples, diluting samples to be tested (composition 1# -5# and composition 1# -3 #) with DMEM culture medium, wherein the diluted concentrations are respectively: 1% (this concentration passes the previous MTT test and is non-toxic). Note that a blank group and a control group are provided, the blank group is not inoculated with cells and the sample to be detected, and the control group is inoculated with cells and is not added with the sample to be detected. After 24 hours of cell culture, the previous DMEM was removed and then carefully washed with DPBS. The DMEM medium added with the sample to be tested prepared in the first step is sequentially added. Cultured in an incubator at 37 ℃ for 48 hours, 5% C02.
(3) MTT staining MTT powder stored in a frozen state was dissolved in DPBS to prepare a solution having a concentration of 2 mg/ml. After 48 hours of culture in 96well dish, 50ul/well of MTT solution was added after removing the medium. Cultured in an incubator at 37 ℃ for 5% Ct) 2 for 3 hours. The MTT solution was completely removed after 3 hours. After complete removal of the MTT solution, 100ul/well of DMSO solution was added to each well separately and shaken on a decolorization shaker for 10 minutes. Absorbance was measured at 540 nm. The cell proliferation rate of each sample was calculated based on the blank.
Calculating the formula: proliferation Rate (%) = { [ (OD-OD) 0 )-(OD 1 -OD 0 )]/(OD 1 -OD 0 ) } × 100%; wherein OD represents the absorbance of the sample, OD 1 Represents the absorbance, OD, of the control group 0 Representing the blank absorbance.
TABLE 6 cell proliferation Effect of compositions 1# to 5# and compositions 1# to 3#
As can be seen from Table 6, the compositions can promote the proliferation of fibroblasts and keratinocytes to different degrees, wherein the compositions No. 1-No. 3 have excellent promoting effects on two groups of test cells, and the proliferation rate is over 50%. Comparing the combination 1 with the combinations 1-3, the effect of compounding the tinospora cordifolia extract, the trehalose and the hydrolyzed hazelnut protein is the best, and if one of the two is lacked, the cell proliferation effect is greatly weakened. Fibroblast proliferation is effective in ameliorating damage to the dermal layers of the skin, while keratinocyte proliferation is effective in repairing damaged stratum corneum. The composition provided by the invention can effectively prevent the further damage of the skin structure by promoting the proliferation of epidermal cells, thereby improving the stability of the skin.
2. Each example and comparative example were tested according to the following test methods.
1. Moisture transdermal loss (TEWL) determination
The magnitude of TEWL is an important indicator for evaluating the strength of the skin barrier function.
Selecting 44 subjects with 20-40 years of dry skin, marking 5 x 5cm on the back of the subjects 2 The test area of (1) and 2-3 days before the test part, any product (cosmetic or external medicine) cannot be used. The environmental temperature is controlled at 25 +/-1 ℃ and the relative humidity is 40 +/-5% during testing, purified water at about 36 ℃ is used for wiping the back of the testee before testing, and the testee is allowed to sit still for 30min under the testing environment for testing. The test area was measured using a capacitance skin tester and recorded as the initial skin water dispersion according to 15 replicates. The subjects bathe once a day with the test samples and measure the TEWL values of the experimental area on days 14 and 28 according to the first measurement method and conditions.
And respectively measuring the TEWL value of each time interval according to the experimental design, and calculating the reduction amount of the skin water dispersion amount of each time point. The greater the reduction in skin moisture loss, the better the skin barrier repair, and the results are shown in Table 7.
Calculating the formula: reduction in skin moisture loss amount = [ (T) 0 -T 1 )/T 0 ]X 100% where T 1 Corresponding TEWL for each time point subject group, where T 0 The TEWL values initially measured for the test area.
TABLE 7 reduction in amount of skin Water Dispersion for examples 1-7 and comparative examples 1-4
As can be seen from Table 7, the bath product added with the soothing and repairing composition can reduce the water dispersion loss of the skin and has a certain repairing effect on the damaged skin barrier after long-term use, and compared with the bath product sold in the market, the repairing effect is more obvious. The best results were obtained in example 1, with a reduction in skin water loss of 16.31% at day 14, which increased with continued use. The skin water dispersion loss of example 2 is generally less than that of example 3 for the same composition ratio and the same composition addition, and the difference may be caused by the irritation of the foaming agent and the cleaning and degreasing force, but the difference is not large. Example 1 a significant concern is that in comparative examples 1-3, it is demonstrated that the addition of a composition that lacks either tinospora cordifolia extract, trehalose, or hydrolyzed hazelnut protein greatly reduces the reparative effect of the composition.
2. And (3) testing the relieving effect:
110 slightly skin-damaged persons of 20-40 years old are selected and divided into 11 groups of 10 persons, and symptoms of the subjects are characterized as follows: dryness, desquamation, itching, erythema, and no significant difference between groups. The subjects bathe the test specimens once a day for 28 consecutive days. Subjects were randomized prior to use and at 28 days of use. Index grading: 0= worsening, 1= slight exacerbation, 2= no significant change, 3= slight improvement, 4= significant improvement, 5= no significant symptoms.
TABLE 8 evaluation of soothing Effect on skin of examples 1 to 7 and comparative examples 1 to 4
As can be seen from table 8, the slightly skin-damaged person had no improvement effect on the damaged skin using the selected general bath cream on the market, while the bath product provided by the present invention to which the composition having the soothing and repairing effect was added had various degrees of soothing effect on the damaged skin. The best effect is shown in example 1, the obvious improvement effect is achieved on dryness, peeling, itching and erythema, and compared with comparative examples 1-3, the improvement difference is obvious, and the added composition has the effect of greatly reducing the relieving effect of the composition if one of the tinospora cordifolia extract, the trehalose or the hydrolyzed hazelnut protein is absent.
It will be appreciated by those of ordinary skill in the art that the examples provided herein are intended to assist the reader in understanding the principles of the invention and are to be construed as being without limitation to such specifically recited examples and embodiments. Those skilled in the art can make various other specific changes and combinations based on the teachings of the present invention without departing from the spirit of the invention, and these changes and combinations are within the scope of the invention.
Claims (7)
1. The bath product is characterized by comprising the following components in percentage by mass:
5 to 30 percent of composition with the effect of relieving and repairing,
10 to 16 percent of foaming agent,
2 to 5 percent of thickening stabilizer,
the balance of other auxiliary agents;
the composition with the relieving and repairing effects comprises the following components in parts by weight:
0.01 to 6.0 parts of tinospora cordifolia extract,
0.01 to 6.0 portions of trehalose,
0.01 to 6.0 parts of hydrolyzed hazelnut protein;
the extraction part of the Tinospora cordifolia extract is selected from Tinospora cordifolia roots and stems;
the Tinospora cordifolia extract is obtained by extracting through an ethanol reflux method: taking dried tubers or roots of Tinospora cordifolia, crushing, adding an ethanol water solution, heating, refluxing, extracting, filtering, concentrating under reduced pressure to obtain a crude extract, purifying the crude extract by adopting macroporous resin, concentrating an eluent into an extract or a solid, adding butanediol, dissolving and filtering, and obtaining a filtrate, namely the Tinospora cordifolia extract;
the composition of the hydrolyzed hazelnut protein is polypeptide with the molecular weight of 300-3000 daltons.
2. The bathing article of claim 1 wherein the soothing and healing composition comprises the following components in parts by weight:
2.0 to 4.0 parts of tinospora cordifolia extract,
2.0 to 4.0 parts of trehalose,
hydrolyzed hazelnut protein 2.0 to 4.0 parts.
3. The bathing article of claim 2 wherein the soothing and healing composition comprises the following components in parts by weight:
2 parts of tinospora cordifolia extract,
4 parts of trehalose, namely 4 parts of trehalose,
3 parts of hydrolyzed hazelnut protein.
4. The bathing article of any one of claims 1 to 3, wherein the soothing and repairing composition is prepared by a method comprising the steps of: mixing the Tinospora cordifolia extract, trehalose and hydrolyzed hazelnut protein to obtain the composition with the effects of relieving and repairing.
5. The bathing article of claim 1, wherein the bathing article comprises the following components in percentage by mass:
10 percent of composition with the function of relieving and repairing,
13 percent of foaming agent,
3.0 percent of thickening stabilizing agent,
the balance of other auxiliary agents.
6. The bath product of claim 1, wherein the foaming agent is one or more of sodium laureth sulfate, decyl glucoside, sodium methylacyl taurate, sodium lauroyl sarcosinate, cocamidopropyl betaine, cocamidopropyl hydroxysultaine, lauramidopropyl betaine, or sodium lauroamphoacetate; the thickening stabilizer is one or more of sodium chloride, cocamide DEA, cocamide methyl MEA, PEG-120 methyl glucose triisostearate or acrylic acid (ester) copolymer.
7. The bathing article of claim 1 wherein the other additives are one or more of water, propylene glycol, glycerin, butylene glycol, phenoxyethanol, benzyl alcohol, sodium benzoate, disodium EDTA, citric acid, or sodium citrate.
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| CN108685741A (en) * | 2018-08-06 | 2018-10-23 | 广州国宇医药科技有限公司 | It is a kind of promote skin barrier reparation composition and its application |
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| JP3268695B2 (en) * | 1993-08-23 | 2002-03-25 | 恒雄 難波 | Cosmetics |
| CN105012203B (en) * | 2015-08-21 | 2017-07-28 | 汕头市雅蒂化妆品有限公司 | Face cream made from a kind of acne-removing composition and use said composition |
| CN106562910A (en) * | 2016-11-14 | 2017-04-19 | 广州蜜妆生物科技有限公司 | Night skin repairing skincare mask |
| CN108553389A (en) * | 2018-06-28 | 2018-09-21 | 芜湖扬展新材料科技服务有限公司 | A kind of plant moisturizing after-sun frost and preparation method thereof |
| CN109498523B (en) * | 2018-12-25 | 2021-07-30 | 广东润洁日化有限公司 | Anti-chapping skin care product and preparation thereof |
| CN110897906A (en) * | 2019-11-19 | 2020-03-24 | 天津郁美净集团有限公司 | Skin barrier repair composition and application thereof |
| CN110840764B (en) * | 2019-11-29 | 2022-06-10 | 广州澳希亚实业有限公司 | Cosmetic composition with long-acting skin-soothing and repairing effects and application thereof |
| CN110787109A (en) * | 2019-12-07 | 2020-02-14 | 路书刚 | Preparation containing peptide for repairing and tightening |
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| CN108685741A (en) * | 2018-08-06 | 2018-10-23 | 广州国宇医药科技有限公司 | It is a kind of promote skin barrier reparation composition and its application |
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