CN112661557A - Plant foliar fertilizer and preparation method and application thereof - Google Patents
Plant foliar fertilizer and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a plant foliar fertilizer and a preparation method and application thereof, belonging to the technical field of agricultural fertilizers, wherein the preparation method of the foliar fertilizer comprises the following steps: 1) crushing animal source raw materials, mixing the crushed raw materials with water, and heating and curing the mixture to obtain cured feed liquid; 2) mixing the cured feed liquid with a composite biological agent for biochemical reaction to obtain a biochemical degradation liquid; the temperature of the biochemical reaction is 50-60 ℃, and the time of the biochemical reaction is preferably 50-70 min; 3) inactivating the composite biological agent in the biochemical degradation liquid, filtering, and collecting filtrate; 4) mixing the filtrate with water and a pH regulator to obtain a plant foliar fertilizer; the solid soluble matter content of the plant foliar fertilizer is more than or equal to 2%, and the pH value is 6.0-9.0; the foliar fertilizer can be absorbed quickly, promote the growth of crops and improve the yield of the crops.
Description
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a plant foliar fertilizer, and a preparation method and application thereof.
Background
Modern agriculture supplements nutrition to plants by means of root fertilization or foliar fertilization, and widely used root fertilizers are chemical fertilizers such as urea and the like. With the understanding of people on the environment and the observation of soil, the damage of the fertilizer to the soil is gradually recognized.
In recent years, field investigation is carried out in counties of agriculture in Guangdong province, part of soil is seriously hardened, soil salinization is serious, the farming and planting industry is seriously threatened, the agriculture hall in Guangdong province also pays attention to the soil problem, the application amount of the fertilizer is not increased in 2020, and is gradually reduced in 2025.
With the use of chemical fertilizers for many years, the natural flavor substance content of vegetables and fruits is greatly reduced, and the fragrant and sweet taste of the prior fruits and vegetables is difficult to eat. Moreover, due to the fact that chemical fertilizers are used too much, the absorption rate of plants is reduced, roots and stems are weak, diseases are easy to generate, the self resistance of the plants is poor, and pesticide is abused, so that the linkage effect is achieved.
The existing foliar fertilizer is mostly obtained by adopting a fixed composition ratio, generally comprises components such as amino acid, trace elements, chemical substances of chemical fertilizers (urea, monopotassium phosphate, ammonium chloride, EDTA and the like), and the like, and has the advantages of high raw material cost, unsatisfactory fertilizer efficiency and certain potential land hazard.
Disclosure of Invention
In view of the above, the invention aims to provide a plant foliar fertilizer which is high in absorption utilization rate, high in absorption speed, free of fertilizer and chemical synthesis components and free of fermentation process treatment, and a preparation method and application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a preparation method of a plant foliar fertilizer, which comprises the following steps:
1) crushing animal source raw materials, mixing the crushed raw materials with water, and heating and curing the mixture to obtain cured feed liquid;
2) mixing the cured feed liquid with a composite biological agent for biochemical reaction to obtain a biochemical degradation liquid; the temperature of the biochemical reaction is 50-60 ℃, and the time of the biochemical reaction is preferably 50-70 min;
3) inactivating the composite biological agent in the biochemical degradation liquid, filtering, and collecting filtrate;
4) mixing the filtrate with water and a pH regulator to obtain a plant foliar fertilizer;
the solid soluble matter content of the plant foliar fertilizer is more than or equal to 2%, and the pH value is 6.0-9.0;
the composite biological preparation comprises the following components in parts by weight: 2-5 parts of pepsin, 15-18 parts of trypsin, 27.5-33 parts of bromelain, 35-41 parts of papain and 10-12 parts of bacillus subtilis cultured extracellular extract.
Preferably, the heating and curing temperature in the step 1) is 85-100 ℃, and the heating and curing time is 50-70 min.
Preferably, the mass ratio of the animal-derived raw materials to water in the step 1) is 1 (4-5).
Preferably, the mass of the compound biological agent is 0.1-0.5% of the mass of the curing feed liquid.
Preferably, the inactivation temperature in the step 3) is 75-85 ℃; the inactivation time is 8-12 min.
Preferably, the particle size of the crushed animal source raw material in the step 1) is 40-80 meshes.
The invention provides a foliar fertilizer prepared by the preparation method, wherein the content of free amino acid in the plant foliar fertilizer is more than or equal to 300mg/L, the content of trace elements is more than or equal to 0.1g/L, the molecular weight of protein peptide in the plant foliar fertilizer is 400-1500D, and the total number of microorganisms in the plant foliar fertilizer is less than or equal to 500 cfu/g.
Preferably, the pH value of the plant foliar fertilizer is 7.0-8.0; the pH value of the foliar fertilizer after being diluted by 250 times with water is 7.0-7.2.
The invention provides application of the foliar fertilizer in improving crop yield.
Preferably, the crops comprise leaf vegetables and melon and fruit vegetables.
The invention has the beneficial effects that: the foliar fertilizer provided by the invention is obtained by crushing, curing and biochemical treatment of animal-derived raw materials, and has the basic properties of pure organic and non-fertilizer compounding; according to the invention, protein molecules in animal-derived raw materials are degraded into small-molecule protein peptides or free amino acids through bipolar decomposition and deep shearing of a composite biological preparation, and the small-molecule protein peptides or free amino acids (the molecular weight is mainly concentrated at 400-1500 daltons) can be actively absorbed by plants through a special channel on the leaf surface, so that the absorption efficiency and the utilization rate of the leaf fertilizer are greatly improved.
Compared with other compound fertilizers, the absorption speed of partial components of the fertilizer is limited, and the absorption quantity of plants is reduced by times in unit time due to ion balance after absorbing a certain quantity, so that the fertility of the plants is easily lost if the plants suddenly rain after use, and even the cycle of the influence of the fertilizer is repeated in soil; the organic components, namely amino acid chelate, macromolecular protein, humic acid or fermentation liquor components have the problem of slow plant absorption speed, and the humic acid and the fermentation liquor components mainly supply a carbon source and regulate the growth environment of microorganisms, so that the organic components are more suitable for root application. The foliar fertilizer provided by the invention is fast in absorption, can be rapidly absorbed by root systems when entering soil in case of raining, can improve soil nutrients, promote the growth of soil microorganisms, activate the activity of root system microorganisms, loosen the soil, and has no residual, secondary pollution or risk of soil hardening.
In addition, as the absorption rate of the plant is improved, the fertilizing amount of the foliar fertilizer is far less than that of other chemical fertilizers, and the plant can rapidly utilize and use the nutrient components in the foliar fertilizer to achieve high-nutrient conditions, so that the plant can grow rapidly; the rich nutrition also stimulates the plant to be faster, more fruits enter the fruit stage, and the plant can breed more fruits and store more nutrients, sugar and flavor substances for the fruits due to the rich nutrition; therefore, the foliar fertilizer has higher crop yield, better quality and better taste.
The plant using the foliar fertilizer disclosed by the invention grows in an environment with rich nutrition, the plant self insect pest resistance is improved, the attack situations of insect pests and diseases are obviously reduced, the attack speed of the diseases is reduced, more treatment time is provided for farmers, the amount of used pesticides is reduced greatly, the pesticide residue is reduced better, the quality of fruits and vegetables is improved, and the safety of organic fruits and vegetables is improved.
Drawings
FIG. 1 is a comparison of a cabbage plant using the foliar fertilizer provided by the present invention and a cabbage plant not using the foliar fertilizer provided by the present invention, wherein the left plant is a plant not using the foliar fertilizer, and the right plant is a plant on which the foliar fertilizer is tried;
FIG. 2 is a comparison of a macaroni field using the foliar fertilizer provided by the present invention and a macaroni field not using the foliar fertilizer provided by the present invention; the left field is the field without foliar fertilizer, and the right field is the field with foliar fertilizer on trial.
Detailed Description
The invention provides a preparation method of a plant foliar fertilizer, which comprises the following steps: 1) crushing animal source raw materials, mixing the crushed raw materials with water, and heating and curing the mixture to obtain cured feed liquid; 2) mixing the cured feed liquid with a composite biological agent for biochemical reaction to obtain a biochemical degradation liquid; the temperature of the biochemical reaction is 50-60 ℃, and the time of the biochemical reaction is preferably 50-70 min; 3) inactivating the composite biological agent in the biochemical degradation liquid, filtering, and collecting filtrate; 4) mixing the filtrate with water and a pH regulator to obtain a plant foliar fertilizer; the solid soluble matter content of the plant foliar fertilizer is more than or equal to 2%, and the pH value is 6.0-9.0; the composite biological preparation comprises the following components in parts by weight: 2-5 parts of pepsin, 15-18 parts of trypsin, 27.5-33 parts of bromelain, 35-41 parts of papain and 10-12 parts of bacillus subtilis cultured extracellular extract.
In the invention, animal source raw materials are crushed and then mixed with water, and the mixture is heated and cured to obtain cured feed liquid. In the invention, the animal-derived raw material is slag of animal origin; the animal source raw materials are not particularly limited, and the animal source raw materials which are conventional in the field can be used; such as the biological residue left in agricultural, fishery and food production processing, such as slaughterhouse biological residue, chicken intestines obtained after chicken killing in chicken raising factories, fish intestine and fish residues obtained after fish killing in aquatic product factories, small fish and shrimps caught in fishery wharfs, broken fish and trash fish, and the like. In the invention, the particle size of the crushed animal source raw material is preferably 40-80 meshes, and more preferably 60 meshes; the crushing is preferably physical crushing; the crushing temperature is preferably 0 to-10 ℃; the method of crushing is not particularly limited in the present invention, and any method of crushing by rolling or crushing by minced meat which is conventional in the art may be used. In the invention, the mass ratio of the animal-derived raw material to water is preferably 1 (4-5), more preferably 1: (4.2-4.8); the heating curing temperature is preferably 85-100 ℃, and the heating curing time is preferably 50-70 min, and more preferably 60 min.
After the curing feed liquid is obtained, mixing the curing feed liquid with a composite biological agent for biochemical reaction to obtain a biochemical degradation liquid; the temperature of the biochemical reaction is preferably 50-60 ℃, and more preferably 52-58 ℃; the time of the biochemical reaction is preferably 50-70 min, more preferably 55-65 min, and most preferably 60 min. In the invention, the composite biological preparation comprises the following components in parts by weight: 2-5 parts of pepsin, 15-18 parts of trypsin, 27.5-33 parts of bromelain, 35-41 parts of papain and 10-12 parts of bacillus subtilis cultured extracellular extract; preferably comprises 3-4 parts of pepsin, 16-17 parts of trypsin, 28-32 parts of bromelain, 36-40 parts of papain and 10.5-11.5 parts of bacillus subtilis cultured extracellular extract. In the invention, the extracellular extract of the bacillus subtilis culture solution is preferably the extracellular extract of the bacillus subtilis culture solution for 45-50 h, and is more preferably the extracellular extract of the bacillus subtilis culture solution for 48 h. In the present invention, the pepsin (5 ten thousand U/g), trypsin (10 ten thousand U/g), bromelain (2 ten thousand U/g) and papain acidocaldarin (20 ten thousand U/g) are preferably commercially available products which are conventional in the art. After the stomach digestive protease, the trypsin, the bromelain and the papain are compounded according to the proportion, the enzyme environment in digestive juice of intestines and stomach of animals can be simulated, and the enzymolysis effect on animal-derived raw materials is the best. The biological enzyme provided by the invention has enzymolysis and digestion effects on animal protein, and can hydrolyze insoluble macromolecular protein into soluble protein. However, the activity and action site of each enzyme are different, so that the efficient digestion and decomposition can be carried out on different protein molecules. The four enzymes have the promotion and supplement functions, and supplement and balance are performed in different pH value ranges, so that the decomposition and digestion can be performed on different protein sites in different pH value control intervals, the rapid decomposition is realized, and the small molecular protein peptide with the molecular weight of 400-1500D is obtained.
After the biochemical degradation liquid is obtained, the composite biological agent in the biochemical degradation liquid is inactivated and filtered, and the filtrate is collected. In the invention, the inactivation temperature is preferably more than or equal to 85 ℃, and the inactivation time is 8-12 min, more preferably 10 min; during the inactivation, stirring is preferably accompanied. After the inactivation step is finished, enzymes in the biochemical degradation liquid and the extracellular extract of the bacillus subtilis culture solution lose biological activity and do not react. After the inactivation and before the filtration, the method also comprises the step of adding a filter aid, wherein the mass of the filter aid is preferably 0.4-0.6 percent of that of the biochemical bionic degradation liquid, and more preferably 0.5 percent; in the present invention, the filter aid is preferably perlite powder; the mass of the filter aid is preferably 0.4-0.6% of the mass of the feed liquid, and more preferably 0.5%; after the filter aid is added, stirring is preferably carried out to mix the filter aid with the feed liquid, and the stirring time is preferably 4-6 min, and more preferably 5 min. In the invention, the filtration is preferably plate-and-frame filtration, and the equipment and parameters of the plate-and-frame filtration are not particularly limited, and the plate-and-frame filtration can be performed by adopting the conventional plate-and-frame filtration in the field. After the filtration, filter residue and filtrate are collected; and after the filter residue is preferably pressed to be dry, the filter residue is used as a solid fertilizer for agricultural production.
In the invention, the filtrate is mixed with water and a pH regulator to obtain the plant foliar fertilizer; the solid soluble matter content of the plant foliar fertilizer is more than or equal to 2%, and the pH value is 6.0-9.0. In the invention, after the filtrate is obtained, the solid soluble content of the filtrate is detected, and the ratio of the filtrate to water is determined according to the detected solid soluble content.
The invention provides a foliar fertilizer prepared by the preparation method, wherein the content of free amino acid in the plant foliar fertilizer is more than or equal to 300mg/L, the content of trace elements is more than or equal to 0.1g/L, the molecular weight of protein peptide in the plant foliar fertilizer is 400-1500D, and the total number of microorganisms in the plant foliar fertilizer is less than or equal to 500 cfu/g. In the invention, the pH value of the plant foliar fertilizer is preferably 7.0-8.0, more preferably 7.2-7.8, and most preferably 7.5; the pH value of the foliar fertilizer after being diluted by 250 times with water is preferably 7.0-7.2. In the invention, the main components of the foliar fertilizer are small molecular animal protein peptide and free amino acid (rich in 18 common plant amino acids), and meanwhile, the foliar fertilizer is rich in three common plant elements of nitrogen, phosphorus and potassium, and the states of the elements exist in a combined manner by biological organic matters; also contains various trace elements required by plant growth: zinc, iron, selenium, copper, boron, magnesium, and the like. The free amino acid in the plant foliar fertilizer is more than or equal to 300mg/L and exceeds the national industry standard by more than 3 times.
The foliar fertilizer provided by the invention adopts pure animal source raw materials, pure organic components, small-molecule protein polypeptide and a large amount of free amino acid, and is beneficial to rapid absorption and utilization of plants. Most of the common foliar fertilizers in the existing market are compound fertilizers, and the compound fertilizers are composed of partial organic components and partial chemical fertilizer components; the foliar fertilizer has the advantages of small dosage, quick absorption, obvious effect, capability of quickly improving the plant resistance and the like.
The invention also provides application of the foliar fertilizer in improving crop yield. In the invention, the crops comprise leaf vegetables and melon and fruit vegetables, and in the specific implementation process of the foliar fertilizer, guava, towel gourd, white melon, water spinach, small vegetable core and eggplant are taken as examples, and the application of the foliar fertilizer is researched. According to usage measurement, the average application amount of the foliar fertilizer provided by the invention is reduced by 30%; the average yield of vegetables and fruits is increased by 30-80%; because the foliar fertilizer improves the resistance of the plant, the incidence rate of diseases is reduced, the influence of the diseases is reduced, the application amount of the pesticide is reduced by 25 percent, and the possibility of pesticide residue is reduced; because no chemical fertilizer is used, the nutrient absorption efficiency of plants is improved, the protein synthesis efficiency is improved, and the content of the taste (flavor substances) of vegetables and fruits is greatly improved.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
1. Raw materials: slaughtering slag material fish sausages of 50kg in a fishery processing factory;
2. pretreatment: freezing the fish sausage raw material, and then physically crushing and stirring to 60 meshes;
3. primary hydrolysis: adding 5 times of water into the crushed raw materials, continuously stirring, heating the materials to 92 ℃, and stewing for 1h at constant temperature;
4. and (3) biochemical degradation: the temperature of the material is reduced, the temperature is controlled to be 53 ℃, 0.02 to 0.65 percent of compound biological agent is added, the stirring is continued, and the biochemical reaction is continued for 1 hour;
5. inactivation: after the biochemical reaction is finished, raising the temperature of the materials to 82 ℃ and maintaining for 10 min;
6. and (3) filtering: performing filter pressing by using a bag filter or a plate frame, filtering out slag materials through filter cloth of a filter screen, and enabling filtrate to enter the next process;
7. blending: measuring the content of solid soluble substances in the material filtrate, and adding water and a pH regulator to obtain the content of the solid soluble substances (protein peptides) of 2.5%; 450kg of foliar fertilizer with the pH value of 7.5;
8. filling and warehousing: filling, packaging and sealing according to the product specification, temporarily storing in a cold storage, wherein the temperature of the cold storage is 4-8 ℃.
Example 2
1. Raw materials: 100kg of slaughtering residue fish intestines and part of dead fish and dead shrimps in a supermarket;
2. pretreatment: carrying out physical crushing and stirring on animal source raw materials to 60 meshes;
3. primary hydrolysis: adding 5 times of water into the crushed raw materials, continuously stirring, heating the materials to above 90 ℃, and steaming at constant temperature for 1 h;
4. and (3) biochemical degradation: reducing the temperature of the materials, controlling the temperature at 57 ℃, adding 0.3 percent of the compound biological agent, continuously stirring, and continuously carrying out biochemical reaction for 1 hour;
5. inactivation: after the biochemical reaction is finished, raising the temperature of the materials to 80 ℃, and maintaining for 10 min;
6. and (3) filtering: performing filter pressing by using a bag filter or a plate frame, filtering out slag materials through filter cloth of a filter screen, and enabling filtrate to enter the next process;
7. blending: measuring the content of solid soluble substances in the material filtrate, and adding water and a pH regulator according to the product design to obtain the content of the solid soluble substances (protein peptides) of 2.8%; 880kg of foliar fertilizer with the pH value of 7.2;
8. filling and warehousing: filling, packaging and sealing according to the product specification, temporarily storing in a cold storage, wherein the temperature of the cold storage is 4-8 ℃.
Example 3
1. Raw materials: 80kg of farmer market slaughter slag materials of chicken intestines and fish intestines;
2. pretreatment: carrying out physical crushing and stirring on animal source raw materials to 60 meshes;
3. primary hydrolysis: adding 4 times of water into the crushed raw materials, continuously stirring, heating the materials to 88 ℃, and stewing for 1 hour at constant temperature;
4. and (3) biochemical degradation: reducing the temperature of the materials, controlling the temperature at 60 ℃, adding 0.4 percent of compound biological agent, continuously stirring, and continuously carrying out biochemical reaction for 1 hour;
5. inactivation: after the biochemical reaction is finished, raising the temperature of the materials to 85 ℃, and maintaining for 10 min;
6. and (3) filtering: performing filter pressing by using a bag filter or a plate frame, filtering out slag materials through filter cloth of a filter screen, and enabling filtrate to enter the next process;
7. blending: measuring the content of solid soluble substances in the material filtrate, and adding water and a pH regulator according to the product design to obtain the content of the solid soluble substances (protein peptides) of 2.4 percent; 810kg of foliar fertilizer with the pH value of 8.0;
8. filling and warehousing: filling, packaging and sealing according to the product specification, temporarily storing in a cold storage, wherein the temperature of the cold storage is 4-8 ℃.
Example 4
1. Raw materials: 110kg of farmer market residue marine trash fish, slightly rotten shrimps, crabs, dead fish and the like;
2. pretreatment: physically crushing and stirring animal source raw materials to 50 meshes;
3. primary hydrolysis: adding 5 times of water into the crushed raw materials, continuously stirring, heating the materials to 92 ℃, and stewing for 1h at constant temperature;
4. and (3) biochemical degradation: reducing the temperature of the materials, controlling the temperature at 55 ℃, adding 0.5% of the compound biological agent, continuously stirring, and continuously carrying out biochemical reaction for 1 h;
5. inactivation: after the biochemical reaction is finished, raising the temperature of the materials to 82 ℃ and maintaining for 10 min;
6. and (3) filtering: performing filter pressing by using a bag filter or a plate frame, filtering out slag materials through filter cloth of a filter screen, and enabling filtrate to enter the next process;
7. blending: measuring the content of solid soluble substances in the material filtrate, and adding water and a pH regulator according to the product design to obtain the content of the solid soluble substances (protein peptides) of 2.7 percent; 650kg of foliar fertilizer with the pH value of 6.8;
8. filling and warehousing: filling, packaging and sealing according to the product specification, temporarily storing in a cold storage, wherein the temperature of the cold storage is 4-8 ℃.
Example 5
The field application effect of the foliar fertilizer is as follows:
and diluting the foliar fertilizer with the pH value of 7.5 and the protein peptide content of 2.5 percent, which is obtained in the embodiment 1, by 60-100 times. The specific application method adopts the application method of the conventional foliar fertilizer in the field.
The effect of guava is shown in table 1.
TABLE 1 trial design and results for guava
The trial effect of the towel gourd is shown in table 2.
Table 2 towel gourd trial design and results
Note: the experimental group uses the foliar fertilizer prepared in the embodiment 1 of the invention; the contrast group uses the common compound water-soluble foliar fertilizer of 'super fish protein amino acid-containing water-soluble fertilizer' in the market.
The trial effect of the white melon is shown in table 3.
TABLE 3 trial design and results for white melon
The trial effect of the water spinach in dry land is shown in table 4.
TABLE 4 trial design and results for dry land swamp cabbage
Note: the experimental group (solid-liquid peptide nutrient solution using group) uses the foliar fertilizer; the contrast group uses the common foliar fertilizer of 'melon and fruit flourishing' in the market.
The trial effect of the small flowering cabbage is shown in table 5.
TABLE 5 trial design and results for small flowering cabbage
Note 1: taking 50 vegetable leaves with similar sizes, taking the average value as the average single leaf weight, and taking mature large leaves as leaves.
Note 2: the experimental group used the foliar fertilizer prepared in the embodiment 1 of the invention; the contrast group uses the common foliar fertilizer of 'melon and fruit flourishing' in the market.
The trial effect of the eggplant is shown in table 6.
TABLE 6 eggplant use design and results
Note: the experimental group used the foliar fertilizer prepared in the embodiment 1 of the invention; the contrast group uses the common foliar fertilizer of 'melon and fruit Wang' in the market.
According to the embodiment, the application amount of the foliar fertilizer is reduced by 30% on average; the average yield of vegetables and fruits is increased by 30-80%; because the foliar fertilizer improves the resistance of the plant, the incidence rate of diseases is reduced, the influence of the diseases is reduced, the application amount of the pesticide is reduced by 25 percent, and the possibility of pesticide residue is reduced; because no chemical fertilizer is used, the nutrient absorption efficiency of plants is improved, the protein synthesis efficiency is improved, and the content of the taste (flavor substances) of vegetables and fruits is greatly improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The preparation method of the plant foliar fertilizer is characterized by comprising the following steps:
1) crushing animal source raw materials, mixing the crushed raw materials with water, and heating and curing the mixture to obtain cured feed liquid;
2) mixing the cured feed liquid with a composite biological agent for biochemical reaction to obtain a biochemical degradation liquid; the temperature of the biochemical reaction is 50-60 ℃, and the time of the biochemical reaction is preferably 50-70 min;
3) inactivating the composite biological agent in the biochemical degradation liquid, filtering, and collecting filtrate;
4) mixing the filtrate with water and a pH regulator to obtain a plant foliar fertilizer;
the solid soluble matter content of the plant foliar fertilizer is more than or equal to 2%, and the pH value is 6.0-9.0;
the composite biological preparation comprises the following components in parts by weight: 2-5 parts of pepsin, 15-18 parts of trypsin, 27.5-33 parts of bromelain, 35-41 parts of papain and 10-12 parts of bacillus subtilis cultured extracellular extract.
2. The method according to claim 1, wherein the temperature of the heating and curing in step 1) is 85 to 100 ℃, and the time of the heating and curing is 50 to 70 min.
3. The preparation method according to claim 1 or 2, wherein the mass ratio of the animal-derived raw material to water in the step 1) is 1 (4-5).
4. The preparation method according to claim 1, wherein the mass of the composite biological agent is 0.1-0.5% of the mass of the aged material liquid.
5. The preparation method according to claim 1, wherein the temperature for inactivation in the step 3) is 75-85 ℃; the inactivation time is 8-12 min.
6. The preparation method according to claim 1, wherein the particle size of the crushed animal-derived raw material in the step 1) is 40 to 80 mesh.
7. The foliar fertilizer prepared by the preparation method of any one of claims 1 to 6, wherein the content of free amino acids in the plant foliar fertilizer is not less than 300mg/L, the content of trace elements is not less than 0.1g/L, the molecular weight of protein peptides in the plant foliar fertilizer is 400-1500D, and the total number of microorganisms in the plant foliar fertilizer is not more than 500 cfu/g.
8. The foliar fertilizer according to claim 7, wherein the pH value of the plant foliar fertilizer is 6.0 to 8.0; the pH value of the foliar fertilizer after being diluted by 250 times with water is 7.0-7.2.
9. Use of a foliar fertiliser as claimed in claim 7 or claim 8 to improve crop yield.
10. The use according to claim 9, wherein the crops comprise leafy vegetables and melon and fruit vegetables.
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|---|---|---|---|---|
| CN1597626A (en) * | 2004-08-27 | 2005-03-23 | 中国海洋大学 | Preparation method of fish protein biological peptide fertilizer |
| CN102076741A (en) * | 2008-04-30 | 2011-05-25 | 希乐克公司 | biomass processing |
| CN106748150A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A method for producing amino acid foliar fertilizer by fermenting low-value marine fish |
| CN106810351A (en) * | 2016-12-23 | 2017-06-09 | 浙江海洋大学 | A method for preparing amino acid foliar fertilizer by hydrolyzing aquatic product by-products |
| CN108558508A (en) * | 2018-05-30 | 2018-09-21 | 芜湖通农生态农业有限公司 | A kind of flower planting amino acid foliage fertilizer |
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- 2019-10-14 CN CN201910973325.0A patent/CN112661557A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1597626A (en) * | 2004-08-27 | 2005-03-23 | 中国海洋大学 | Preparation method of fish protein biological peptide fertilizer |
| CN102076741A (en) * | 2008-04-30 | 2011-05-25 | 希乐克公司 | biomass processing |
| CN106748150A (en) * | 2016-12-23 | 2017-05-31 | 浙江海洋大学 | A method for producing amino acid foliar fertilizer by fermenting low-value marine fish |
| CN106810351A (en) * | 2016-12-23 | 2017-06-09 | 浙江海洋大学 | A method for preparing amino acid foliar fertilizer by hydrolyzing aquatic product by-products |
| CN108558508A (en) * | 2018-05-30 | 2018-09-21 | 芜湖通农生态农业有限公司 | A kind of flower planting amino acid foliage fertilizer |
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