CN112611861A - 一种荧光免疫检测芯片及其制备方法 - Google Patents
一种荧光免疫检测芯片及其制备方法 Download PDFInfo
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- CN112611861A CN112611861A CN202011323877.6A CN202011323877A CN112611861A CN 112611861 A CN112611861 A CN 112611861A CN 202011323877 A CN202011323877 A CN 202011323877A CN 112611861 A CN112611861 A CN 112611861A
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- G—PHYSICS
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01233736A (ja) * | 1988-03-15 | 1989-09-19 | Fujitsu Ltd | 半導体装置の製造方法 |
CN1645147A (zh) * | 2005-01-18 | 2005-07-27 | 南开大学 | 用于免疫学分析的蛋白质芯片 |
US20080011934A1 (en) * | 2006-06-30 | 2008-01-17 | Asml Netherlands B.V. | Imprint lithography |
US20080182070A1 (en) * | 2007-01-31 | 2008-07-31 | Pei-Yu Chou | Method of manufacturing an imprinting template using a semiconductor manufacturing process and the imprinting template obtained |
US20080209817A1 (en) * | 2007-01-05 | 2008-09-04 | Kinik Company | Diamond polishing disc process |
CN104880447A (zh) * | 2015-06-09 | 2015-09-02 | 吉林大学 | 一种新型ZnO NRs/Au膜复合三维微阵列生物芯片基底及其制备方法 |
CN104923324A (zh) * | 2015-07-03 | 2015-09-23 | 南京理工大学 | 一种基于光敏树脂固化成型的pdms微流控芯片制备方法 |
CN105044076A (zh) * | 2015-06-24 | 2015-11-11 | 吉林大学 | 一种背面检测式表面增强拉曼散射芯片及其制备方法 |
CN105706222A (zh) * | 2013-11-21 | 2016-06-22 | 株式会社尼康 | 布线图案的制造方法和晶体管的制造方法 |
CN106115609A (zh) * | 2016-06-29 | 2016-11-16 | 中国科学院重庆绿色智能技术研究院 | 微通孔列阵癌细胞检测芯片紫外压印制作方法 |
CN110187091A (zh) * | 2019-04-18 | 2019-08-30 | 浙江大学 | 一种用于抗肿瘤药物筛选的高通量3d细胞阻抗传感器及检测方法 |
CN110699634A (zh) * | 2019-09-11 | 2020-01-17 | 神通科技集团股份有限公司 | 一种塑件表面处理方法 |
CN111141796A (zh) * | 2020-02-24 | 2020-05-12 | 张洮 | 微波电容传感器及被测物介电特性和绝对位置的测量方法 |
CN111610323A (zh) * | 2020-06-03 | 2020-09-01 | 武汉世纪康敏生物科技有限公司 | 一种等离激元增强荧光免疫检测芯片及其应用 |
CN111921574A (zh) * | 2020-08-03 | 2020-11-13 | 杭州欧光芯科技有限公司 | 一种真空光感键合微流控生物芯片的封装方法 |
CN215963614U (zh) * | 2020-12-20 | 2022-03-08 | 北京康敏生物科技有限公司 | 一种免疫检测芯片 |
-
2020
- 2020-11-23 CN CN202011323877.6A patent/CN112611861B/zh active Active
Patent Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01233736A (ja) * | 1988-03-15 | 1989-09-19 | Fujitsu Ltd | 半導体装置の製造方法 |
CN1645147A (zh) * | 2005-01-18 | 2005-07-27 | 南开大学 | 用于免疫学分析的蛋白质芯片 |
US20080011934A1 (en) * | 2006-06-30 | 2008-01-17 | Asml Netherlands B.V. | Imprint lithography |
US20080209817A1 (en) * | 2007-01-05 | 2008-09-04 | Kinik Company | Diamond polishing disc process |
US20080182070A1 (en) * | 2007-01-31 | 2008-07-31 | Pei-Yu Chou | Method of manufacturing an imprinting template using a semiconductor manufacturing process and the imprinting template obtained |
CN105706222A (zh) * | 2013-11-21 | 2016-06-22 | 株式会社尼康 | 布线图案的制造方法和晶体管的制造方法 |
CN104880447A (zh) * | 2015-06-09 | 2015-09-02 | 吉林大学 | 一种新型ZnO NRs/Au膜复合三维微阵列生物芯片基底及其制备方法 |
CN105044076A (zh) * | 2015-06-24 | 2015-11-11 | 吉林大学 | 一种背面检测式表面增强拉曼散射芯片及其制备方法 |
CN104923324A (zh) * | 2015-07-03 | 2015-09-23 | 南京理工大学 | 一种基于光敏树脂固化成型的pdms微流控芯片制备方法 |
CN106115609A (zh) * | 2016-06-29 | 2016-11-16 | 中国科学院重庆绿色智能技术研究院 | 微通孔列阵癌细胞检测芯片紫外压印制作方法 |
CN110187091A (zh) * | 2019-04-18 | 2019-08-30 | 浙江大学 | 一种用于抗肿瘤药物筛选的高通量3d细胞阻抗传感器及检测方法 |
CN110699634A (zh) * | 2019-09-11 | 2020-01-17 | 神通科技集团股份有限公司 | 一种塑件表面处理方法 |
CN111141796A (zh) * | 2020-02-24 | 2020-05-12 | 张洮 | 微波电容传感器及被测物介电特性和绝对位置的测量方法 |
CN111610323A (zh) * | 2020-06-03 | 2020-09-01 | 武汉世纪康敏生物科技有限公司 | 一种等离激元增强荧光免疫检测芯片及其应用 |
CN111921574A (zh) * | 2020-08-03 | 2020-11-13 | 杭州欧光芯科技有限公司 | 一种真空光感键合微流控生物芯片的封装方法 |
CN215963614U (zh) * | 2020-12-20 | 2022-03-08 | 北京康敏生物科技有限公司 | 一种免疫检测芯片 |
Non-Patent Citations (2)
Title |
---|
DONG QUAN 等: "Significantly enhanced structural integrity of adhesively bonded PPS and PEEK composite joints by rapidly UV-irradiating the substrates", COMPOSITES SCIENCE AND TECHNOLOGY, vol. 199 * |
李娜;曹一平;张玮莹;刘继延;尤庆亮;于洁;申超;郭文勇;: "紫外光固化在微流控芯片技术中的应用研究进展", 江汉大学学报(自然科学版), no. 02 * |
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