CN112603928A - Disinfectant and detergent co-production method and disinfectant - Google Patents
Disinfectant and detergent co-production method and disinfectant Download PDFInfo
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- CN112603928A CN112603928A CN202011476443.XA CN202011476443A CN112603928A CN 112603928 A CN112603928 A CN 112603928A CN 202011476443 A CN202011476443 A CN 202011476443A CN 112603928 A CN112603928 A CN 112603928A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/20—Elemental chlorine; Inorganic compounds releasing chlorine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/14—Alkali metal chlorides; Alkaline earth metal chlorides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/02—Inorganic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/20—Halogens; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/25—Silicon; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/02—Inorganic compounds
- C11D7/04—Water-soluble compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/02—Inorganic compounds
- C11D7/04—Water-soluble compounds
- C11D7/10—Salts
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/02—Inorganic compounds
- C11D7/04—Water-soluble compounds
- C11D7/10—Salts
- C11D7/12—Carbonates bicarbonates
Abstract
The invention discloses a co-production method of a disinfectant and a detergent, which comprises the following steps: step 1, placing a sea salt solution with the concentration of 0.0005 (five parts per million) in a diaphragm electrolytic cell for electrolysis at the temperature of 4 ℃; step 2, adding 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent into 100ml of alkaline solution; after being mixed evenly, the detergent is obtained; step 3, standing the acidic solution for 14-18 days; and 4, taking 100ml of the acid solution after being placed in the step 3, adding 0.1mg of silicate and 0.05mg of carbomer, and uniformly mixing to obtain the disinfectant. The invention can produce the disinfectant and the detergent simultaneously, improves the production process and reduces the cost. The prepared disinfectant has the effects of inhibiting and killing various bacteria, viruses and certain moulds, and has no irritation or damage to human bodies within a proper pH range and concentration range; and has no irritation to skin, vaginal mucosa, oral mucosa and spectacle mucosa.
Description
Technical Field
The invention belongs to the field of medical biochemistry, and particularly relates to a disinfectant and detergent co-production method and a disinfectant.
Background
A plurality of studies at home and abroad prove that the oxidation-reduction potential water has wide bactericidal spectrum and quick sterilization, and can kill not only bacterial propagules and viruses, but also various pathogenic microorganisms such as spores and the like.
The laboratory sterilization test results show that: the oxidation-reduction potential water stock solution (without organic matter) can completely kill Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus (including MRSA), gonococcus and other bacteria propagules after 15-30 seconds; can completely destroy hepatitis B surface antigen and kill HIV after 30 seconds of action; the action lasts for 10-20 minutes, and the spores of the black variety of the bacillus subtilis can be killed.
The application suspension test result shows that when the ratio of the oxidation-reduction potential water to the bacterial suspension is more than 50:1, the ratio of the oxidation-reduction potential water to the bacterial suspension is as follows: the killing time of H7, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens and Vibrio parahaemolyticus is less than 10 seconds.
The main effective component of oxidation-reduction potential water sterilization is hypochlorous acid. It has four sterilization effects:
1. reacting with intracellular protoplasm to form a toxic compound N-CL;
2. N-CL binds with cell membrane and protein, prevents cell metabolism, and causes abnormal cell membrane permeability;
3. cell oxidative death;
4. inhibiting enzyme activity, sugar metabolism and energy metabolism.
In the production of current oxidation-reduction potential water (disinfectant), one is to electrolyze a saline solution without a diaphragm, and the final product has a lot of alkaline ions, so that the sterilization and disinfection effects are poor. The other method is to separate the alkaline solution by diaphragm electrolysis of the saline solution, but the direct discharge after the separation can damage the environment, so the further neutralization and precipitation treatment is needed, and the production cost is increased.
Disclosure of Invention
The invention aims to solve the technical problem of providing a co-production method of a disinfectant and a detergent aiming at the defects in the prior art, which can simultaneously produce the disinfectant and the detergent, improves the production process and reduces the cost.
The invention discloses a co-production method of a disinfectant and a detergent, which comprises the following steps:
step 1, placing a sea salt solution with the concentration of 0.0005 (five parts per million) in a diaphragm electrolytic cell for electrolysis at the temperature of 4 ℃; the cathode product is an acidic solution, and the anode product is an alkaline solution;
step 2, adding 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent into 100ml of alkaline solution; after being mixed evenly, the detergent is obtained;
step 3, standing the acidic solution for 14-18 days;
and 4, taking 100ml of the acid solution after being placed in the step 3, adding 0.1mg of silicate and 0.05mg of carbomer, and uniformly mixing to obtain the disinfectant.
In the co-production method of the disinfectant and the detergent, when the step 1 is electrolyzed, the current is 10A-30A, the potential is less than or equal to 1100mv, the PH of the electrolyzed acidic solution is controlled to be 5.0-6.8, and the electrolysis speed is controlled to be 150 liters per hour.
In the co-production method of the disinfectant and the detergent, when the step 2 is operated, firstly, 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent are added into 40ml of alkaline solution and uniformly mixed; then 60ml of alkaline solution is added and mixed evenly.
The invention discloses a disinfectant in a second aspect, which comprises the following components: edible sea salt, sodium hypochlorite, hypochlorous acid, silicate, carbophilus and pure water;
the mass percentage of each component is as follows: 0.32% of edible sea salt, 0.035% of sodium hypochlorite, 0.065% of hypochlorous acid, 0.05% of silicate, 0.06% of carbopol and 99.47% of pure water.
In the third aspect of the invention, the oral cavity gargle is disclosed, the pH value of the disinfectant is adjusted to 6.0-6.8, the content of available chlorine is adjusted to 70-80 ppm, and the available chlorine refers to hypochlorous acid.
The invention discloses a children oral collutory, wherein the disinfectant is adjusted to have the pH value of 6.0-6.8, the effective chlorine content is adjusted to be 40-50 ppm, and the effective chlorine refers to hypochlorous acid.
The fifth aspect of the invention discloses a gynecological lotion, wherein the pH value of the disinfectant is adjusted to 4.5-6.8, the content of available chlorine is adjusted to 110-120 ppm, and the available chlorine refers to hypochlorous acid.
Compared with the prior art, the invention has the following advantages:
1. the co-production method of the disinfectant and the detergent improves the production process, reduces the production cost, ingeniously recycles the alkaline solution to prepare the detergent, changes waste into valuable, and creates economic benefit. In addition, the electrolytic voltage, the electric potential and the ambient temperature are controlled, so that the content of available chlorine in the prepared acidic solution is increased.
2. The disinfectant prepared by the invention can reach the medicinal grade, and is safe and reliable.
3. The oral collutory, children oral collutory and gynecological lotion provided by the invention have the effects of inhibiting and killing various bacteria, viruses and certain moulds, and have no stimulation and damage to human bodies within a proper pH range and concentration range; and has no irritation to skin, vaginal mucosa, oral mucosa and spectacle mucosa; when used as a gargle, the product can not stain teeth and tongue; if the disinfectant is taken by children by mistake, the intestinal tract of the children is not stimulated and damaged, the disinfection effect is good, and the disinfectant is a strong disinfectant.
The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.
Drawings
FIG. 1 is a flow diagram of a co-production process of the present invention.
Detailed Description
As shown in fig. 1, a co-production method of disinfectant and detergent comprises the following steps:
step 1, placing a sea salt solution with the concentration of 0.0005 (five parts per million) in a diaphragm electrolytic cell for electrolysis at the temperature of 4 ℃; the cathode product is an acidic solution, and the anode product is an alkaline solution; during electrolysis, the current is 10A-30A, the potential is less than or equal to 1100mv, the PH of the electrolyzed acidic solution is controlled to be 5.0-6.8, and the electrolysis speed is controlled to be 150 liters per hour;
step 2, adding 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent into 100ml of alkaline solution; after being mixed evenly, the detergent is obtained; specifically, 40ml of alkaline solution is firstly added with 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent and uniformly mixed; then 60ml of alkaline solution is added and further mixed evenly; the foaming agent is used in the conventional liquid detergent;
step 3, standing the acidic solution for 14-18 days; the reason for the standing is to stabilize the acidic solution;
and 4, taking 100ml of the acid solution after being placed in the step 3, adding 0.1mg of silicate and 0.05mg of carbomer, and uniformly mixing to obtain the disinfectant.
The disinfectant prepared by the method comprises the following components: edible sea salt, sodium hypochlorite, hypochlorous acid, silicate, carbophilus and pure water;
the mass percentage of each component is as follows: 0.32% of edible sea salt, 0.035% of sodium hypochlorite, 0.065% of hypochlorous acid, 0.05% of silicate, 0.06% of carbopol and 99.47% of pure water.
When the disinfectant is used as oral cavity gargle, the pH of the disinfectant is adjusted to 6.0-6.8, the content of available chlorine is adjusted to 70-80 ppm, and the available chlorine refers to hypochlorous acid.
When the disinfectant is used as children oral collutory, the disinfectant has pH of 6.0-6.8, and effective chlorine content of 40-50 ppm, wherein the effective chlorine is hypochlorous acid.
The pH value of the oral cavity of the human body is adjusted to be 6.0-6.8.
When the disinfectant is used as gynecological lotion, the disinfectant has pH of 4.5-6.8 and effective chlorine content of 110-120 ppm, wherein the effective chlorine is hypochlorous acid.
The pH value of the pH value is adjusted to be 4.5-6.8, which is the pH value of human vagina.
It is to be noted that, the prior mouthwash before oral cavity diagnosis mainly adopts chlorhexidine hydrochloride (chlorhexidine) mouthwash; to clean and disinfect the oral cavity, and then to perform oral surgery and treatment. The oral liquid has the defects that the oral liquid is prepared from chemical raw materials (medicinal grade), has slight irritation to skin, vaginal mucosa and oral mucosa, can stain teeth, turn yellow and black to the tongue when used as a gargle for the tongue of teeth, has irritation and damage to intestinal tracts if a child swallows the oral liquid by mistake, has a common disinfection effect, and is a weak disinfection product.
When the disinfectant is used as the oral mouthwash, the skin, the vaginal mucosa, the oral mucosa and the eye mucosa are not irritant and harmless to human bodies.
The samples referred to in the following experimental examples are the above disinfectants.
Experimental example 1 (the disinfectant has no damage to chromosome)
Animal and environment
1. Animals: SPF grade KM mice, 50, female halves. Certificate number: no.11072620H000455, supplied by experimental animal breeding limited of shandong province, china, denying: SCXK (lu) 20190003.
2. Environmental conditions: the SPF-level animal house enables an open license number to be obtained by an experimental animal: SYXK (Lu) 20190004, room temperature 19.6 deg.C, 23.4 deg.C; the relative humidity is 43.9-58.6%.
Second, method
I. The detection basis is as follows: disinfection technical Specification (2002 year edition)
2. Preparation of a test substance:
high dose group: accurately weighing 12.50g of a sample into a 50ml volumetric flask, adding pure water to a constant volume to a scale mark, fully shaking up, pouring into a beaker for later use, and obtaining a final concentration of about 250 mg/ml.
The medium dose group: accurately measuring 5ml of prepared sample solution of the high-dose group, adding pure water to dilute the sample solution to lOml, and uniformly mixing to obtain a final concentration of about 125 mg/ml.
Low dose group: the prepared sample solution of the high-dose group was accurately measured 2 ml. Diluting to 10m with pure water, and mixing to obtain final concentration of about 50 mg/ml.
Positive control group: 0.02g of cyclophosphamide is accurately weighed, 10m 1m pure water is added to completely dissolve the cyclophosphamide, and the mixture is uniformly mixed to obtain the final concentration of 2 mg/ml.
Negative control group: administration of the corresponding solvent
3. The test method comprises the following steps:
3.1, animal grouping: 50 KM mice qualified for quarantine are selected and divided into 5 dose groups according to a weight and size machine, namely a high dose group, a medium dose group, a low dose group, a positive control group and a negative control group. 10 animals per group, male and female halves.
3.2, dosage design: half lethal dose LD of this product50>5000mg/kg, therefore a high dose of 5000mg/kg, a medium dose of 1/2 at the high dose, and a low dose of 1/5 at the high dose were designed.
3.3, a contamination method: the drug is contaminated by oral gavage, 20ml/kg, 2 times of drug contamination are carried out at intervals of 24h, and the materials are obtained 6h after the second drug contamination.
The animals were sacrificed by cervical dislocation and the femurs were removed. The muscles were stripped off and the blood stain was wiped off. Cutting off two ends of femur, exposing marrow cavity, and making into tablet.
3.4, micronucleus count:
selecting the area with uniform and complete cell distribution and proper coloring. The number of the chromophilous red blood cells (PCE) containing micronuclei is counted under an oil lens, the PCE is grayish blue (mature red blood cells (NCE) are pink), the micronuclei are mostly in a shape of a member, the edges are smooth and regular, the chromophily is consistent with the nuclear cytoplasm of the nucleated cells, and the micronuclei are purple or bluish purple, and the diameters of the micronuclei are generally 1/20-1/5 of red blood cells.
Each animal was counted for 1000 PCEs. Micronucleus cell rate refers to the number of PCEs containing micronuclei, expressed in thousandths. There are 2 or more microkernels present in 1 PCE, still counting by 1. In addition, the PCE/NCE ratio should be observed as an indicator of cytotoxicity. Typically 200 PCEs are counted while counting the NCE seen. When the PCE/NCE is less than 0.1, the obvious inhibition effect on bone marrow is suggested, the dosage of the test object is reduced, and the test is carried out again.
3.5, evaluation rules:
statistical treatment is performed using the poisson distribution u test or other suitable significance testing method. When each dosage group is compared with a solvent control group, the increase of the micronucleus cell rate has significance and has dosage-response relation, or only one dosage group has significance in the increase of the micronucleus cell rate, and repeated experiments prove that the test object has the function of in-vivo chromosome damage.
Three, result in
The low dose group, the medium dose group and the high dose group have no significant difference (P >0.05) compared with the negative control group, and the positive control group has significant difference (P <0.05) compared with the negative control group by using the Poisson distribution u test.
Mouse marrow pleochromocyte micronucleus test result (n ═ 10)
Note: p < 0.01, compared to negative control group; p <0.05, compared to negative control group;
fourth, conclusion
Under the experimental condition, according to the judgment standard of micronucleus test in technical Specification for Disinfection (2002 edition), the sample has no in vivo chromosome damage effect on KM mice, and belongs to mutation-causing negativity.
Experimental example 2 (the disinfectant has no irritation to skin)
Animal and environment
1. Animals: 3 common grade New Zealand rabbits, female. Certificate number: no.11082320H000088, supplied by qingdakang biotechnology limited, shandong province, experimental animal production license number: SCXK (lu) 20160002.
2. Environmental conditions: ordinary grade animal house, the license number is used to the experimental animals: SYXK (lu) 20190004, room temperature 20.5 deg.C, 24.9 deg.C; relative humidity 41.3% -65.5%.
Second, method
1. The detection basis is as follows: disinfection technical Specification (2002 year edition)
2. Sample preparation: used as received
3. The test method comprises the following steps:
3.1, preparing skin: 24h before infection, the furs on both sides of the dorsal spinal cage of the New Zealand rabbit are removed by using a hair removing agent, so that the skin cannot be damaged. The range of the depilated surface is not less than 3cmx3 cm.
3.2, a contamination method, adopting a same body left-right self contrast method, directly dripping 0.5ml of stock solution on the left unhaired complete skin with the area of 2.5cmx2.5cm, or dripping on 2 layers and 4 layers of gauze with the same size and applying on the surface of the left unhaired skin, then covering with a layer of non-irritant cellophane, and fixing with a non-irritant adhesive plaster. The right skin was treated with sodium chloride injection as a negative control. The application time is 4h, after the application is finished, the contaminated part is gently cleaned by warm water, and residual water stains are wiped off by absorbent paper. The application is carried out once a day for 14 d.
And 3.3, observing the result 24 hours after each smearing, and scoring according to the table 1. To facilitate the application of the test substance and
results observations were made that clipping was necessary. The control zone is treated in the same manner as the test zone.
3.4, evaluation rules:
the average integral per animal per day (irritation index) was calculated according to the following formula, and the skin irritation intensity was judged in Table 2.
TABLE 1 skin irritation response Scoring criteria
TABLE 2
| Score value | Evaluation of |
| 0～＜0.5 | Has no irritation |
| 0.5～＜2.0 | Light irritation |
| 2.0～＜6.0 | Moderate irritation |
| 6.0～＜8.0 | Strong irritation |
Three, result in
The skin of the infected side and the skin of the control side of the experimental animals do not show any skin erythema and edema symptoms within 14 days of infection, and the average integral of the infected side and the control side of each animal is 0 point each day.
Fourth, conclusion
Under the experimental condition, according to the skin irritation strength classification of disinfection technical specification (2002 edition), the irritation strength of the sample to the multiple intact skins of New Zealand rabbits is nonirritant.
Experimental example 3 (the disinfectant has no irritation to eyes)
The experiment was carried out in accordance with the disinfection specification (2002) in the same manner as in example 2, and it was concluded that under the experimental conditions, the sample was non-irritating to the type of injury caused by rabbit eyes in New Zealand according to the eye irritation response grading Standard in the disinfection specification (2002).
Experimental example 4 (the disinfectant has no irritation to damaged skin)
The experiment was carried out in accordance with the "Disinfection Specification" (2002 edition) in the same manner as in example 2, and it was concluded that the sample was non-irritating to the skin of New Zealand rabbits with one-time damage according to the evaluation standard for skin irritation intensity in accordance with the "Disinfection Specification" (2002 edition) under the present experimental conditions.
EXAMPLE 5 (the disinfectant is practically non-toxic)
Experiments were carried out in the same manner as in experimental example 1, according to the "Disinfection protocol" (2002 edition), with the conclusion that under the conditions of the experiment, the sample was practically non-toxic to acute oral toxicity in hermaphroditic mice.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, changes and equivalent structural changes made to the above embodiment according to the technical spirit of the present invention still fall within the protection scope of the technical solution of the present invention.
Claims (7)
1. A co-production method of a disinfectant and a detergent is characterized by comprising the following steps:
step 1, placing a sea salt solution with the concentration of 0.0005 (five parts per million) in a diaphragm electrolytic cell for electrolysis at the temperature of 4 ℃; the cathode product is an acidic solution, and the anode product is an alkaline solution;
step 2, adding 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent into 100ml of alkaline solution; after being mixed evenly, the detergent is obtained;
step 3, standing the acidic solution for 14-18 days;
and 4, taking 100ml of the acid solution after being placed in the step 3, adding 0.1mg of silicate and 0.05mg of carbomer, and uniformly mixing to obtain the disinfectant.
2. A combined disinfectant and detergent process according to claim 1, wherein: during the electrolysis in the step 1, the current is 10A-30A, the potential is less than or equal to 1100mv, the PH of the electrolyzed acidic solution is controlled to be 5.0-6.8, and the electrolysis speed is controlled to be 150 liters per hour.
3. A co-production process of a disinfectant and detergent composition as claimed in claim 1 or 2, wherein: when the step 2 is operated, firstly, 2.08g of edible baking soda, 1.2g of edible salt and 1.4ml of foaming agent are added into 40ml of alkaline solution and uniformly mixed; then 60ml of alkaline solution is added and mixed evenly.
4. A disinfectant prepared by the co-production process of a disinfectant and a detergent as claimed in any one of claims 1 to 3, comprising the following components: edible sea salt, sodium hypochlorite, hypochlorous acid, silicate, carbophilus and pure water;
the mass percentage of each component is as follows: 0.32% of edible sea salt, 0.035% of sodium hypochlorite, 0.065% of hypochlorous acid, 0.05% of silicate, 0.06% of carbopol and 99.47% of pure water.
5. An oral mouthwash, characterized in that: the disinfectant as claimed in claim 4, wherein the disinfectant has a pH of 6.0-6.8 and an effective chlorine content of 70-80 ppm, and the effective chlorine is hypochlorous acid.
6. A children oral collutory is characterized in that: the disinfectant as claimed in claim 4, wherein the disinfectant has a pH of 6.0-6.8 and an effective chlorine content of 40-50 ppm, and the effective chlorine is hypochlorous acid.
7. A gynecological lotion is characterized in that: the disinfectant as claimed in claim 4, wherein the disinfectant has a pH of 4.5-6.8 and an effective chlorine content of 110-120 ppm, and the effective chlorine is hypochlorous acid.
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Application publication date: 20210406 |