CN113207905A

CN113207905A - Contact lens care composition and preparation method thereof

Info

Publication number: CN113207905A
Application number: CN202110536912.0A
Authority: CN
Inventors: 王青松; 李笑微; 张柳青; 顾鹏鹏; 徐敏
Original assignee: Hangzhou Weixiao Medical Technology Co ltd; Shanghai Aikangte Medical Technology Co ltd; Zhuhai Xigu Medical Technology Co ltd; Zhuhai Weishi Aikangte Pharmaceutical Technology Co ltd
Current assignee: Hangzhou Weixiao Medical Technology Co ltd; Shanghai Aikangte Medical Technology Co ltd; Zhuhai Xigu Medical Technology Co ltd; Zhuhai Weishi Aikangte Pharmaceutical Technology Co ltd
Priority date: 2021-05-17
Filing date: 2021-05-17
Publication date: 2021-08-06

Abstract

The present disclosure describes a nursing composition for a contact lens and a preparation method thereof, wherein the nursing composition comprises a bactericide, a buffer, an isotonic regulator, a traditional Chinese medicine extracting solution with a bacteriostatic action and water, the bactericide is hydrogen peroxide, the traditional Chinese medicine extracting solution is one selected from honeysuckle extracting solution, scutellaria extracting solution, moutan bark extracting solution, wormwood extracting solution and dark plum extracting solution, in the nursing composition, the mass volume ratio of the bactericide is 1% to 8%, the volume ratio of the traditional Chinese medicine extracting solution is 10% to 50%, the concentration of the traditional Chinese medicine extracting solution is 1g per 1mL of the traditional Chinese medicine, the pH value of the nursing composition is 5 to 8, the osmotic pressure of the nursing composition is 270 to 330mOsm/kg, the nursing composition is in a liquid or gel state, and the nursing composition is subjected to sterilization treatment. According to the present disclosure, a contact lens care composition having an antibacterial effect and a method for producing the same can be provided.

Description

Contact lens care composition and preparation method thereof

Technical Field

The present disclosure relates generally to a care composition for contact lenses and a method of making the same.

Background

In recent years, with the increase in the number of people wearing contact lenses, the demand for contact lens care products has become more urgent. The contact lens care generally includes cleaning, disinfection, and storage, wherein the cleaning is to remove deposits, debris, microorganisms, and biofilms on the contact lens surface, and the disinfection is to further kill and remove microorganisms on the contact lens by moist heat or chemical disinfection, and the storage is used for preventing contamination.

The disinfection of the contact lenses can be realized by hydrogen peroxide, because bacteria are very easily attached to the contact lenses, the action principle of the hydrogen peroxide is that hydroxyl with high activity is dissociated through complex chemical reaction and acts on cell membranes, the strong oxidizing property of the hydrogen peroxide destroys proteins forming microorganisms to cause the microorganisms to die, and the residual substances after the microorganisms are killed by the hydrogen peroxide are water without any toxicity or any irritation, so that secondary pollution is not formed.

However, the current hydrogen peroxide care solution often has no continuous bacteriostatic ability, and generally has no bactericidal or bacteriostatic ability after hydrogen peroxide is converted into water and oxygen free radicals, so that the care solution with the antibacterial effect needs to be actively developed.

Disclosure of Invention

The present disclosure has been made in view of the above-mentioned state of the art, and an object thereof is to provide a contact lens care composition having an antibacterial effect and a method for producing the same.

For this purpose, the disclosure provides a nursing composition for a contact lens, which comprises a bactericide, a buffer, an isotonic regulator, a traditional Chinese medicine extracting solution with a bacteriostatic action and water, wherein the bactericide is hydrogen peroxide, the traditional Chinese medicine extracting solution is selected from one of honeysuckle extracting solution, scutellaria baicalensis extracting solution, moutan bark extracting solution, wormwood extracting solution and dark plum extracting solution, in the nursing composition, the mass-to-volume ratio (w/v) of the bactericide is 1% to 8%, the volume-to-volume ratio (v/v) of the traditional Chinese medicine extracting solution is 10% to 50%, the concentration of the traditional Chinese medicine extracting solution is 1g of the traditional Chinese medicinal material per 1mL, the pH value of the nursing composition is 5 to 8, the osmotic pressure of the nursing composition is 270 to 330mOsm/kg, and the nursing composition is in a liquid or gel state. In the disclosure, hydrogen peroxide is used as a bactericide to play a role in disinfection and sterilization, a traditional Chinese medicine extract with a bacteriostatic action is used to achieve the purpose of long-acting bacteriostasis, and the bactericide, a buffering agent, an isotonic regulator and the traditional Chinese medicine extract act synergistically to endow a nursing composition with a antibacterial action, so that the nursing composition has a long-term disinfection and sterilization effect.

In addition, in the care composition related to the present disclosure, optionally, the buffer is selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid, and acetate, and the mass volume ratio of the buffer is not more than 10%. Thereby, the pH of the care composition can be controlled within a physiologically acceptable range.

Additionally, in the care composition to which the present disclosure relates, optionally, the buffering agent is selected from at least one of boric acid, sodium tetraborate, phosphoric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, citric acid, sodium citrate, and trisodium citrate.

Additionally, in the care compositions to which the present disclosure relates, optionally, the isotonicity adjusting agent is selected from at least one of sodium chloride, potassium chloride, calcium chloride, propylene glycol, and glycerin. Thereby, the osmolality of the care composition can be adjusted to the target osmolality.

In addition, in the care composition related to the present disclosure, optionally, in the care composition, the mass volume ratio (w/v) of the bactericide is 3% to 3.5%, and the volume ratio (v/v) of the chinese medicine extract is 20% to 30%. Therefore, the sterilization and disinfection effects and the bacteriostatic effect of the nursing composition can be enhanced.

Further, in the care composition according to the present disclosure, optionally, the pH of the care composition is 6 to 7, and the osmolality of the care composition is 280 to 320 mOsm/kg. This can contribute to reducing the irritation to the eyeball and to reducing the adverse effect on the eyeball.

In another aspect, the present disclosure provides a method of preparing a care composition for a contact lens, comprising: preparing a traditional Chinese medicine extracting solution and adding the traditional Chinese medicine extracting solution into water for mixing, then respectively adding a bactericide, a buffering agent and an isotonic regulator for mixing, and then performing sterilization treatment to obtain the nursing composition, wherein the traditional Chinese medicine extracting solution is selected from one of honeysuckle extracting solution, scutellaria baicalensis extracting solution, moutan bark extracting solution, wormwood extracting solution and dark plum extracting solution, the bactericide is hydrogen peroxide, in the nursing composition, the mass-to-volume ratio (w/v) of the bactericide is 1-8%, the volume-to-volume ratio (v/v) of the traditional Chinese medicine extracting solution is 10-50%, the concentration of the traditional Chinese medicine extracting solution is 1g of the traditional Chinese medicine per 1mL, the pH value of the nursing composition is 5-8, the osmotic pressure is 270-330 mOsm/kg, and the nursing composition is in a liquid or gel state. In the present disclosure, a care composition having an antibacterial effect can be obtained by mixing a bactericide, a buffer, an isotonicity adjusting agent, and a Chinese medicinal extract in a certain ratio and allowing the respective components to act synergistically.

In addition, in the preparation method according to the present disclosure, optionally, the step of preparing the chinese medicine extract solution includes: soaking the Chinese medicinal materials in ethanol or water for a certain time, extracting at a certain temperature for a certain time, filtering, collecting filtrate, soaking the residue in ethanol or water for a certain time, extracting at a certain temperature for a certain time, filtering, mixing the filtrates, and concentrating. Thus, the Chinese medicinal extract with appropriate concentration can be obtained.

In addition, in the preparation method according to the disclosure, optionally, during the soaking of the traditional Chinese medicinal material, the volume ratio concentration of ethanol used is not lower than 95%, and the mass/volume ratio (w/v) of the traditional Chinese medicinal material to ethanol or water is 1:3 to 1: 20. Therefore, the extraction of the traditional Chinese medicine extracting solution can be facilitated.

In addition, in the preparation method according to the present disclosure, optionally, the buffer is selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid, and acetate, and the isotonic adjusting agent is selected from at least one of sodium chloride, potassium chloride, calcium chloride, propylene glycol, and glycerin. Thus, care compositions having a pH and osmotic pressure within physiologically acceptable ranges can be prepared.

According to the present disclosure, a contact lens care composition having an antibacterial effect and a method for producing the same can be provided.

Drawings

Embodiments of the present disclosure will now be explained in further detail, by way of example only, with reference to the accompanying drawings, in which:

fig. 1 is a flow chart illustrating a method of making a treatment composition for a contact lens according to examples of the present disclosure.

Fig. 2 is a flowchart illustrating a method of preparing a chinese medicine extracting solution according to an example of the present disclosure.

Detailed Description

The present disclosure will be described in further detail below with reference to the accompanying drawings and specific embodiments. In the drawings, the same components or components having the same functions are denoted by the same reference numerals, and redundant description thereof will be omitted.

The contact lens care composition according to the present embodiment (hereinafter referred to as "care composition") can be used for cleaning, disinfecting, storing, and the like of contact lenses. The contact lens may be a contact lens, for example, the contact lens may be a soft or hard corneal contact lens, a soft or hard scleral contact lens, a keratoplasty lens, or the like.

In some examples, the treatment composition may be used as a contact lens wearing solution, a contact lens care solution, a contact lens disinfecting solution, a contact lens preserving solution, a contact lens cleaning solution, or a multifunctional care solution. In the present embodiment, the dosage form of the care composition is not particularly limited, and for example, the care composition may be in a liquid or gel form.

The treatment composition according to the present embodiment may be catalyzed and neutralized with a metal catalyst (e.g., platinum, gold, silver). For example, platinum and contact lenses may be added to the treatment composition, with the catalytic decomposition of platinum being used to effect simultaneous (hydrogen peroxide) catalytic decomposition and contact lens disinfection. In some examples, contact lenses treated with the care composition can be worn directly. Alternatively, the contact lens treated with the care composition may be rinsed with a rinsing agent (e.g., saline) prior to wearing the contact lens.

In some examples, the treatment composition may be disinfected and neutralized in steps. For example, the contact lens may be disinfected with the treatment composition and then neutralized with a neutralizing agent (e.g., catalase).

In some examples, the treatment composition may be used in conjunction with a cleaning device to achieve better cleaning and/or disinfecting. For example, the cleaning device can be used in combination with a cleaning device to achieve the effect of removing proteins (proteins attached to the lens). In other examples, the cleaning device used in conjunction with the treatment composition may include a metal catalyst or neutralizing agent.

In some examples, the treatment composition may include an antimicrobial agent. In addition, bactericides may be used to kill microorganisms. For example, the bactericide can kill microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Fusarium solanacearum. In some examples, the sterilant may be hydrogen peroxide (sometimes also referred to as "hydrogen peroxide"). Thereby, bacteria and fungi can be effectively killed.

In some examples, the bactericide may be present in the care composition at a mass to volume ratio (w/v) of 1% to 8%. For example, the mass to volume ratio (w/v) of the bactericide may be 1%, 2%, 3%, 4%, 5%, 6%, 7%, or 8%.

In some examples, the bactericide may preferably have a mass to volume ratio (w/v) of 3% to 3.5%. Thereby, the disinfecting action of the care composition can be advantageously enhanced.

In some examples, the treatment composition may include a buffering agent. In addition, the buffering agent may adjust the pH of the treatment composition and may maintain the pH of the treatment composition within a certain range. For example, the pH of the care composition may be within a physiologically acceptable range.

In some examples, the buffering agent may maintain the pH of the treatment composition between 5 and 8. In this case, it is possible to improve the stability of the care composition, to reduce the irritation to the eyeball, and to reduce the adverse effect on the eyeball. That is, the pH of the treatment composition may be from 5 to 8. For example, the pH of the treatment composition may be 5, 5.2, 5.5, 5.7, 6, 6.3, 6.5, 6.8, 7, 7.5, or 8.

In some examples, the pH of the treatment composition may preferably be from 6 to 7. In this case, the pH of the care composition is near neutral, which can be beneficial in reducing irritation to the eye and can help reduce adverse effects on the eye.

In some examples, the buffering agent may be present in the treatment composition in an amount effective to maintain the pH of the treatment composition within a target range (e.g., pH 5 to 8).

In some examples, the buffer may have a mass to volume ratio (w/v) of no greater than 10% in the care composition. Thereby, the pH of the care composition can be controlled within a physiologically acceptable range. For example, the buffer may have a mass to volume ratio (w/v) of 0.01%, 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%.

In some examples, the buffer may be selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid, and acetate. For example, the buffering agent may be selected from at least one of boric acid, sodium tetraborate, phosphoric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, citric acid, sodium citrate, and trisodium citrate.

In some examples, the treatment composition may include an isotonic adjusting agent. In addition, the isotonicity adjusting agent can adjust the osmotic pressure of the treatment composition. For example, the osmolality of the care composition can be adjusted within a physiologically acceptable range. In other examples, the osmolality of the treatment composition may be about the same as the osmolality of a 0.9% sodium chloride solution.

In some examples, the isotonic adjusting agent may adjust the osmolality of the treatment composition to 270 to 330 mOsm/kg. Therefore, the device can meet the physiological requirements of human bodies, reduce the stimulation to eyeballs and reduce the adverse effect on the eyeballs. That is, the osmolality of the treatment composition can be 270 to 330 mOsm/kg. For example, the osmolality of the treatment composition can be 270, 275, 280, 285, 290, 300, 310, 320, or 330 mOsm/kg.

In some instances, preferably, the osmolality of the treatment composition may be 280 to 320 mOsm/kg. This can contribute to reducing the irritation to the eyeball and to reducing the adverse effect on the eyeball.

In some examples, in the treatment composition, the isotonic adjusting agent may be present in an amount effective to adjust the osmolality of the treatment composition within a target range (e.g., an osmolality of 270 to 330 mOsm/kg).

In some examples, the mass to volume ratio (w/v) of the isotonicity adjusting agent in the care composition may be 0.5% to 3%.

In some examples, the isotonic adjusting agent may be selected from at least one of sodium chloride, potassium chloride, calcium chloride, propylene glycol, and glycerin. Thereby, the osmolality of the care composition can be adjusted to the target osmolality.

In some examples, the treatment composition may include herbal extracts. In addition, the Chinese medicinal extract has antibacterial effect. For example, the extractive solution can inhibit Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Salmonella.

In some examples, the herbal extract may be selected from one of honeysuckle extract, scutellaria extract, moutan extract, wormwood extract and smoked plum extract. This can inhibit growth and propagation of microorganisms.

In some examples, the volume ratio (v/v) of the chinese herbal medicine extract in the care composition may be 10% to 50%. Thus, growth and propagation of microorganisms can be effectively inhibited. In addition, the concentration of the traditional Chinese medicine extracting solution can be 1g per 1mL of the traditional Chinese medicine.

In some examples, the concentration of the herbal extract may be 10%, 15%, 20%, 30%, 40%, or 50% by volume (v/v) in the treatment composition.

In some examples, in the care composition, preferably, the volume ratio (v/v) of the chinese medicine extract may be 20% to 30%. Therefore, the antibacterial effect can be enhanced.

In some examples, the treatment composition may have water as a solvent. In other words, the treatment composition may comprise water. In other examples, the water may be distilled water, purified water, sterile purified water, water for injection, distilled water for injection, or the like.

In some examples, the treatment composition may further include additives, selected according to actual needs. For example, the treatment composition may include additives that enhance the germicidal and disinfectant effects, additives that accelerate the rate of neutralization, and the like.

In some examples, the treatment composition may be comprised of a bactericide, a buffer, an isotonicity adjusting agent, a herbal extract, and water. In other examples, the treatment composition may be composed of a bactericide for killing microorganisms, a buffer for maintaining the pH of the treatment composition at 5 to 8, an isotonic regulator for adjusting the osmotic pressure of the treatment composition to 270 to 330mOsm/kg, a herbal extract having bacteriostatic action, and water.

In some examples, bactericides, buffers, isotonicity adjusting agents and herbal extracts may act synergistically in the care compositions. For example, bactericides, buffers, isotonicity adjusting agents and herbal extracts may act synergistically to provide antimicrobial action to the care composition; the bactericide, the buffer, the isoosmotic adjusting agent and the traditional Chinese medicine extracting solution can act synergistically to enhance the effects of sterilization and disinfection of the nursing composition and the like.

In the embodiment, hydrogen peroxide is used as a bactericide to perform the disinfection and sterilization effects, the traditional Chinese medicine extract with the bacteriostasis effect is used to achieve the purpose of long-acting bacteriostasis, and the bactericide, the buffering agent, the isotonic regulator and the traditional Chinese medicine extract act synergistically to endow the nursing composition with the antibacterial effect, so that the nursing composition has the long-term disinfection and sterilization effects.

Hereinafter, referring to fig. 1, a method for preparing a care composition for a contact lens according to the present disclosure will be described in detail. Fig. 1 is a flow chart illustrating a method of making a treatment composition for a contact lens according to examples of the present disclosure. Fig. 2 is a flowchart illustrating a method of preparing a chinese medicine extracting solution according to an example of the present disclosure.

In the present embodiment, as shown in fig. 1, the method for preparing the care composition for a contact lens may include preparing a herbal extract, adding the herbal extract to water, and mixing (step S100). In addition, the Chinese medicinal extract has antibacterial effect.

In some examples, the temperature of the water added to the chinese medicine extracting solution may be between 30 and 40 ℃ in step S100. Therefore, the dispersion of the traditional Chinese medicine extracting solution in water can be facilitated. For example, the temperature of water may be 30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃, 35 ℃, 36 ℃, 37 ℃, 38 ℃, 39 ℃ or 40 ℃.

In some examples, in step S100, mixing may be performed by stirring, ultrasound, or the like, so as to disperse (mix) the chinese medicine extract in water. In some examples, in step S100, the mixing time may be 5 to 30min (minutes).

In some examples, as described above, in step S100, preparing chinese medicine extract may be included. In addition, in some examples, as shown in fig. 2, the step of preparing the chinese medicine extract may include soaking the chinese medicine material in ethanol for a certain time, then extracting at a specific temperature for a predetermined time, and then filtering to collect the filtrate (step S101).

In some examples, the concentration (v/v) of ethanol may be not less than 95% in step S101. Therefore, the extraction of the traditional Chinese medicine extracting solution can be facilitated. For example, the concentration of ethanol may be 95%, 96%, 97%, 98%, 99%, or 100%.

In some examples, the mass to volume ratio (w/v, g: ml) of the Chinese herbal material to ethanol in step S101 can be 1:3 to 1: 20. Therefore, the extraction of the traditional Chinese medicine extracting solution can be facilitated. For example, the mass to volume ratio of the traditional Chinese medicine to the ethanol may be 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, or 1: 20.

In some examples, in step S101, the soaking time of the Chinese herbal medicine may be 1 to 6 hours (hours). For example, the soaking time of the traditional Chinese medicinal materials can be 1h, 2h, 3h, 4h, 5h or 6 h.

In some examples, in step S101, the specific temperature (extraction temperature) may be 25 ℃ to 80 ℃. For example, the specific temperature may be 25 ℃, 30 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃.

In some examples, in step S101, the extraction time of the Chinese herbal medicine may be 0.5 to 8 hours. For example, the extraction time may be 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, or 8 h.

In some examples, in step S101, the filtrate and the filter residue may be separated by filtration, wherein the filtrate may be used for concentration and the filter residue may be repeatedly extracted.

In some examples, in step S101, screen filtration, plate-and-frame filtration, … … centrifugal filtration may be employed.

In some examples, in step S100, as shown in fig. 2, the step of preparing the chinese medicine extracting solution may include soaking the residue in ethanol for a certain time, and then filtering after extracting at a specific temperature for a predetermined time (step S102). In other words, in step S102, the residue separated in step S101 may be extracted again.

In some examples, in step S102, the soaking time of the filter residue may be the same as the soaking time of the Chinese herbal medicine in step S101. In addition, in some examples, the extraction temperature in step S102 may be the same as the extraction temperature in step S101. In other examples, the extraction time in step S102 may be the same as the extraction time in step S101.

In some examples, in step S100, as shown in fig. 2, the step of preparing the chinese medicine extracting solution may include combining the two filtrates and concentrating (step S103). Thus, the Chinese medicinal extract with appropriate concentration can be obtained.

In some examples, in step S103, the concentration may be performed by rotary evaporation, atmospheric evaporation, reduced pressure evaporation, membrane evaporation, freeze concentration, reverse osmosis, ultrafiltration techniques, centrifugal concentration, or the like.

In some examples, in step S103, the extract may be concentrated to 1g of the original herb per 1 mL.

In the present embodiment, as shown in fig. 1, the method for preparing the care composition for a contact lens may include separately adding and mixing an antimicrobial agent, a buffer agent, and an isotonicity adjusting agent (step S200).

In some examples, in step S200, the mixing may be performed by stirring, ultrasound, or the like. In addition, in some examples, the order of addition of the bactericide, the buffer, and the isotonic reagent in step S200 is not particularly required.

In some examples, the biocide may have a biocidal effect. Additionally, in some examples, the sterilant may be hydrogen peroxide. In other examples, the buffer may be selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid, and acetate. Thus, care compositions having a pH value in a physiologically acceptable range can be prepared.

In some examples, the isotonic adjusting agent may be selected from at least one of sodium chloride, potassium chloride, calcium chloride, propylene glycol, and glycerin. Thus, care compositions having an osmotic pressure within a physiologically acceptable range can be prepared. In addition, in some examples, the herbal extract may be selected from one of honeysuckle extract, scutellaria extract, moutan bark extract, wormwood extract and smoked plum extract.

In some examples, in step S200, the pH may be adjusted to a target range (e.g., pH 5 to 8) by adding an effective amount of buffer.

In some examples, in step S200, the osmolality can be adjusted to a target range (e.g., osmolality of 270 to 330mOsm/kg) by adding an effective amount of an isotonic adjusting agent.

In some examples, in step S200, adding an additive may be further included according to actual requirements.

In the present embodiment, as shown in fig. 1, the method for preparing the care composition for a contact lens may include performing a sterilization treatment to obtain the care composition (step S300).

In some examples, in step S300, the sterilization process may be performed by moist heat sterilization, filter sterilization, radiation sterilization, or ultraviolet sterilization. Thereby, a sterile care composition can be obtained.

In some examples, in step S300, in the care composition, the mass-to-volume ratio (w/v) of the bactericide may be 1% to 8%, the volume ratio (v/v) of the chinese herb extract may be 10% to 50%, and the concentration of the chinese herb extract is 1g per 1mL equivalent to the original chinese herb, and the bactericide, the buffer, and the isotonic regulator cooperate with the chinese herb extract to provide the care composition with an antibacterial effect. Thus, a contact lens care composition having an antibacterial effect can be prepared. In addition, in some examples, in step S300, the pH of the treatment composition may be 5 to 8 and the osmolality may be 270 to 330 mOsm/kg.

In the present embodiment, a care composition having an antibacterial effect can be prepared by mixing a bactericide, a buffer, an isotonicity adjusting agent, and a Chinese medicinal extract at a certain ratio and allowing each component to act synergistically.

To further illustrate the present disclosure, the contact lens care compositions provided by the present disclosure are described in detail below with reference to examples, and the benefits achieved by the present disclosure are fully illustrated with reference to comparative examples.

In this example and comparative example, the extraction procedure of the herbal extract was as follows: soaking 100g of Chinese medicinal materials in 500ml of 95% ethanol for 4h, extracting at 30 deg.C for 1 hr, filtering, collecting filtrate, soaking filter residue in 500ml of 95% ethanol for 4h, extracting at 30 deg.C for 1 hr, mixing filtrates, and concentrating the filtrate to 100ml by rotary evaporation.

[ examples ]

First, the raw materials of the examples (examples 1 to 17) were prepared according to table 1, and the herbal extracts were added to purified water at 30 ℃ and dispersed for 10min, then added with the bactericide, buffer and isotonicity adjusting agent, and then subjected to ultrasonic mixing, filtration using an ultrafiltration column and a filter membrane, and irradiation sterilization to obtain the care compositions of examples 1 to 17 (50 ml for each example) as shown in table 1.

Next, the care compositions obtained in the respective examples (examples 1 to 17) were subjected to a bactericidal effect test, a hydrogen peroxide residual amount test, a viscosity test, and a bacteriostatic ability test. The specific detection process is as follows:

(1) test of sterilizing Effect

Collecting fresh culture (18-24 h) of strain 3-14 generation nutrient agar slant, washing with 0.03mol/L Phosphate Buffer Solution (PBS), and diluting to obtain culture with bacteria content of 10⁷cfu/mL-10⁸cfu/mL of bacterial suspension.

10.0mL of three sterilized test tubes containing human are respectively sucked from the three batches of samples and put in a water bath at 20-25 ℃ for 5 min.

Respectively adding 0.1mL of bacterial suspension into the test tubes to make the final bacterial content be 10⁷cfu/mL-10⁸cfu/mL, mix well and start timing.

Transferring 1.0mL of mixed bacteria and medicines (mixed liquor of bacterial suspension and nursing composition) into 9.0mL of neutralizing agent respectively for four different action times (recommended to be 25%, 50%, 75% and 100% of shortest disinfection time), mixing uniformly, neutralizing for 10min, absorbing 1.0mL of stock solution or 10 times of serial diluent, respectively inoculating nutrient agar medium (bacteria) and Saburg agar medium (yeast), inoculating two plates for each pipe, respectively culturing the bacteria and mother bacteria at 35-37 ℃ for 48h (bacteria) or 72h (yeast), counting viable bacteria, and taking the average value.

The sample was replaced with 0.03mol/L PBS, and viable bacteria were counted by the same method as described above as a positive control. Taking PBS and 1.0mL of neutralizing agent to inoculate a nutrient agar culture medium or a Saburg agar culture medium and the culture medium which is not inoculated to be used as a negative control, and calculating the killing rate of each bacterium at each action time point according to a formula. Wherein the killing rate is (viable count of positive control group-viable count of test group)/viable count of positive control group multiplied by 100%, when the elimination rate to bacteria is more than or equal to 99.90%, and the elimination rate to yeast is more than or equal to 90.00%, the product is judged to be qualified. The results are shown in Table 3.

(2) Residual quantity test of hydrogen peroxide

The treatment composition is neutralized for 60 minutes (i.e., platinum catalyzed for 60 minutes), and a neutralized sample is taken from 5.0mL to 150mL in a conical flask, added with 5mL of 10% sulfuric acid and 10mL of distilled water, titrated with a 0.002mol/L potassium permanganate standard solution, shaken steadily, titrated to pink and held for at least 15 seconds. At the same time, blank control was performed.

Hydrogen peroxide concentration (mg/kg) ═ V-V₀)×c×170.1/(0.02×50)……(I)

In the formula (I), V is the volume of the potassium permanganate solution consumed by the sample, wherein the volume is 0.002mol/L, and the unit is mL; v₀The volume of 0.002mol/L potassium permanganate solution is consumed as a blank control, and the unit is mL; and c is the molar concentration of the potassium permanganate standard solution, and the unit is mol/L.

And when the residual quantity of hydrogen peroxide is less than 0.2 percent, the hydrogen peroxide basically finishes the reaction of the nursing composition after platinum is catalyzed for 60 min. The results are shown in Table 3.

(3) Viscosity test

The measurement was carried out according to "viscometry" third method "rotational viscometer method" in pharmacopoeia of the people's republic of China (2015 edition).

The viscosity is 1-7 mPa & s, when the viscosity is less than 1mPa & s, the retention time of the care composition on the lens is short, and dirt on the lens is not cleaned; when the viscosity is more than 7 mPas, the retention time of the care composition on the lens is increased, which is disadvantageous for rinsing and future use of the lens.

(4) Test of bacteriostatic ability

After the nursing composition is catalyzed by platinum for 6 hours, the nursing composition can be basically considered to be free of hydrogen peroxide, and at the moment, the traditional Chinese medicine extracting solution mainly provides bacteriostatic ability. The cup and dish method is adopted to measure the bacteriostatic activity of the care solution for 24 hours, and the bacteriostatic activity of the traditional Chinese medicine extracting solution in each formula can be reflected. Specifically, agar medium is poured into a culture dish, 0.1ml of bacterial suspension with the concentration of 100CFU/ml is sucked after solidification, the bacterial suspension is uniformly coated on a flat plate, three sterile oxford cups are equidistantly placed on the flat plate, 200ml of nursing solution is added into each oxford cup, absolute ethyl alcohol is used as a control, and each sample is subjected to 3 parallel tests. The cells were incubated at 37 ℃ for 24 hours, and the diameter of the zone of inhibition was measured and recorded. The results are shown in Table 3.

For the diameter of the zone of inhibition, larger means stronger inhibition. When the diameter of the inhibition zone is more than 10mm, the nursing composition can be regarded as having remarkable inhibition function.

TABLE 1

[ comparative example ]

Raw materials of respective comparative examples (comparative example 1 to comparative example 17) were prepared according to table 2, and then care compositions of respective comparative examples were prepared in the same manner as in examples, and a bactericidal effect test, a hydrogen peroxide residual amount test, a viscosity test, and a bacteriostatic ability test were performed in the same manner as in examples. The test results are shown in Table 3.

TABLE 2

TABLE 3

As can be seen from table 3, the solutions of examples (examples 1 to 17) all have excellent performance in the bactericidal effect test, the hydrogen peroxide residual amount test, the viscosity test and the bacteriostatic ability test. And the comparative example fails to pass a sterilization effect test, a hydrogen peroxide residual quantity test, a viscosity test and a bacteriostatic ability test at the same time.

According to various preferred embodiments, the compositions of the present invention are suitable for disinfecting soaked contact lenses, which products, after neutralization, are well biocompatible with the eye. In conventional contact lens soaking and disinfecting solutions, the antimicrobial agent is present in an amount effective to disinfect the contact lens, with the preferred amount of disinfection being a reduction in the microbial load on a log scale over a period of time.

The larger the content of the hydrogen peroxide is, the more ideal the disinfection effect is, but the overlarge concentration can cause overlarge residual quantity of the hydrogen peroxide, thereby influencing the use safety of the contact lenses. The nursing composition provided by the invention has the mass volume ratio of the bactericide of 1-8%. Can provide excellent sterilizing effect without generating excessive bactericide residue.

The larger the proportion of the traditional Chinese medicine extract is, the stronger the subsequent antibacterial activity is. When the proportion of the traditional Chinese medicine extracting solution is small, the subsequent bacteriostasis effect cannot be achieved, and meanwhile, the viscosity of the nursing composition is low, so that the detention time of the nursing composition on the contact lenses is short, and the cleaning of the contact lenses is not facilitated; when the proportion of the Chinese herbal medicine extract is large, the viscosity of the nursing composition is too high, the residence time of the nursing composition on the contact lens is increased, and the contact lens is not easy to wash and use in the future. The traditional Chinese medicine extracting solution contained in the nursing composition provided by the invention can provide a bacteriostatic function, wherein the bacteriostatic ability of the dark plum extracting solution is optimal. According to the nursing composition provided by the invention, the volume ratio of the traditional Chinese medicine extracting solution is 10-50%, so that the viscosity of the nursing composition can be in a proper range.

While the present disclosure has been described in detail in connection with the drawings and examples, it should be understood that the above description is not intended to limit the disclosure in any way. Those skilled in the art can make modifications and variations to the present disclosure as needed without departing from the true spirit and scope of the disclosure, which fall within the scope of the disclosure.

Claims

1. The nursing composition for the contact lenses is characterized by comprising a bactericide, a buffering agent, an isoosmotic adjusting agent, a traditional Chinese medicine extracting solution with a bacteriostatic action and water, wherein the bactericide is hydrogen peroxide, the traditional Chinese medicine extracting solution is selected from one of honeysuckle extracting solution, scutellaria baicalensis extracting solution, moutan bark extracting solution, wormwood extracting solution and dark plum extracting solution, in the nursing composition, the mass-volume ratio of the bactericide is 1-8%, the volume ratio of the traditional Chinese medicine extracting solution is 10-50%, the concentration of the traditional Chinese medicine extracting solution is 1g of the traditional Chinese medicine per 1mL, the pH value of the nursing composition is 5-8, the osmotic pressure of the nursing composition is 270-330 mOsm/kg, the nursing composition is in a liquid or gel state, and the nursing composition is subjected to sterilization treatment.

2. The care composition of claim 1,

the buffer is selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid and acetate, and the mass volume ratio of the buffer is not more than 10%.

3. The care composition of claim 1 or 2,

the buffer is selected from at least one of boric acid, sodium tetraborate, phosphoric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, citric acid and trisodium citrate.

4. The care composition of claim 1,

the isotonic regulator is at least one selected from sodium chloride, potassium chloride, calcium chloride, propylene glycol and glycerin.

5. The care composition of claim 1,

in the nursing composition, the mass volume ratio of the bactericide is 3-3.5%, the volume ratio of the traditional Chinese medicine extracting solution is 20-30%, and the traditional Chinese medicine extracting solution is selected from one of scutellaria baicalensis extracting solution, wormwood extracting solution and dark plum extracting solution.

6. The care composition of claim 1,

the pH value of the care composition is from 6 to 7 and the osmolality of the care composition is from 280 to 320 mOsm/kg.

7. A method for preparing a care composition for contact lenses,

the method comprises the following steps: preparing Chinese medicinal extractive solution, adding into water, mixing, adding bactericide, buffer and isotonic regulator, mixing, sterilizing to obtain the care composition,

the nursing composition comprises a traditional Chinese medicine extracting solution, a bactericide, a pH value of 5-8 and an osmotic pressure of 270-330 mOsm/kg, wherein the traditional Chinese medicine extracting solution is selected from one of honeysuckle extracting solution, scutellaria baicalensis extracting solution, moutan bark extracting solution, wormwood extracting solution and dark plum extracting solution, the bactericide is hydrogen peroxide, in the nursing composition, the mass volume ratio of the bactericide is 1-8%, the volume ratio of the traditional Chinese medicine extracting solution is 10-50%, the concentration of the traditional Chinese medicine extracting solution is 1g of the original traditional Chinese medicine per 1mL, the pH value of the nursing composition is 5-8, and the osmotic pressure is 270-330 mOsm/kg, and the nursing composition is in a liquid or gel state.

8. The method according to claim 7,

the step of preparing the traditional Chinese medicine extracting solution comprises the following steps: soaking the Chinese medicinal materials in ethanol or water for a certain time, extracting at a certain temperature for a certain time, filtering, collecting filtrate, soaking the residue in ethanol or water for a certain time, extracting at a certain temperature for a certain time, filtering, mixing the filtrates, and concentrating.

9. The method according to claim 8,

in the process of soaking the traditional Chinese medicinal materials, the volume ratio concentration of ethanol is not lower than 95%, and the mass volume ratio of the traditional Chinese medicinal materials to the ethanol or the water is 1:3 to 1: 20.

10. The method according to claim 7,

the buffer is selected from at least one of boric acid, borate, phosphoric acid, phosphate, citric acid, citrate, carbonic acid, carbonate, acetic acid and acetate, and the isotonic regulator is selected from at least one of sodium chloride, potassium chloride, calcium chloride, propylene glycol and glycerol.