JP3624915B2 - Disinfectant for barns and poultry houses - Google Patents
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Description
【０００１】
【産業上の利用分野】
本発明は畜舎及び鶏舎用の殺菌消毒剤に関する。
【０００２】
【従来の技術】
畜舎および鶏舎の消毒殺菌は，家畜の伝染病の伝播や感染を防ぐと共に飼育に伴って生じる飼育環境の微生物汚染を排除し家畜の正常な成育を維持するために不可欠となっている。
【０００３】
従来、畜舎及び鶏舎の消毒殺菌には、アルコール又はアルデヒド製剤、石炭酸又はクロロフェノール製剤、クレゾール製剤、逆性石鹸製剤、両性石鹸製剤、ハロゲン塩製剤、ｏ−ジクロロベンゼンを主成分とするオルソ剤等の薬剤が使用されている。
【０００４】
しかしながら、上記の薬剤には抗菌スペクトルが限られていたり、培養菌液では効果があっても実際の鶏舎に散布した場合、菌数の減少率が１０−１程度にしかならない等の欠点がある（古田賢治「養鶏施設における消毒に関する諸問題」日本家禽学会誌，３０，ｐ．３２５ −３３５（１９９３） ）。また、散布場所周辺の環境、排水等への悪影響が懸念される薬剤も多い。そこで、十分な消毒殺菌効果を発揮する、安全、安定かつ安価な畜舎及び鶏舎用消毒殺菌剤が望まれている。
【０００５】
畜産分野で問題となる疾病のうち、鶏コクシジウム症の被害が大きいが、その疾病対策は困難である。コクシジウム症は、Ｅｉｍｅｒｉａ属に属する原虫であるコクシジウム原虫によって引き起こされる。コクシジウムはオーシストの状態で動物に感染し、動物体内で虫体になった後、腸管上皮細胞に寄生して増殖する。その後、虫体がオーシストとなり、糞と共に動物体外に放出される。動物体外に放出された直後のオーシストは胞子を形成していないので感染力を持たないが、好適な条件で数日経過すると胞子を形成して感染力を発揮する。
【０００６】
すなわち、コクシジウム症はオーシストによって伝播し、オーシストを殺滅できなければコクシジウム症の伝播を防止できない。しかしながら、その感染源となるオーシストは一般の殺菌消毒剤に対して強い抵抗性を有している。
【０００７】
オーシストに対して効果がある殺菌消毒剤としてはｏ−ジクロロベンゼンを主成分とするオルソ剤が知られているが、オルソ剤の場合、オーシスト以外の細菌に対しては殺菌作用が弱く、また、散布場所周辺の環境に対する悪影響が懸念される。
【０００８】
この為、オーシスト殺滅効果を持ち、オーシスト以外の細菌に対しても優れた殺菌効果を示す、安全、安定かつ安価な薬剤の開発が望まれている。
【０００９】
【本発明が解決しようとする課題】
本発明の第一の目的は、一般の細菌に対して十分な消毒殺菌効果を発揮する、安全、安定かつ安価な畜舎及び鶏舎用消毒殺菌剤の提供である。本発明の第二の目的は、オーシストに対して強い殺滅効果がある畜舎及び鶏舎用殺菌消毒剤の提供である。
【００１０】
【課題を解決するための手段】
本発明者らは、前述の課題を解決するために鋭意検討を重ねた結果、過カルボン酸を有効成分とする薬剤が畜舎及び鶏舎用殺菌消毒剤として有効であることを見いだした。
【００１１】
本発明で使用する過カルボン酸としては、過蟻酸、過酢酸及び過プロピオン酸が例示されるが、中でも過酢酸が好ましい。過カルボン酸は殺菌効果に優れ、しかも散布後は比較的容易に分解する為に環境中に残留せず、また、毒性も低いので環境に対する悪影響が小さいという特長を有している。
【００１２】
本発明の畜舎及び鶏舎用殺菌消毒剤は、様々な剤型で使用可能だが、好ましくは水溶液の形で使用する。
【００１３】
一般の細菌（常在菌）に対して消毒殺菌効果を期待する場合、成分濃度が以下のような水溶液が好適に使用される。すなわち、過カルボン酸の濃度は０．０４〜４０％（水溶液中の重量％；以下同じ）、好ましくは０．１０〜１０％である。過カルボン酸は水溶液中で過酸化水素及びカルボン酸と平衡状態にあるので、本発明の畜舎及び鶏舎用殺菌消毒剤は過カルボン酸の他に、過酸化水素及びカルボン酸を含み得る。過酸化水素の濃度は０．０１〜３０％、好ましくは０．１０〜１０％である。カルボン酸の濃度は０．０５〜４０％、好ましくは０．１０〜２０％である。
【００１４】
過カルボン酸濃度は、小方芳郎「有機過酸化物の化学」南江堂（１９７１）ｐ．３３１−３４３ を参考に、過マンガン酸カリウムとチオ硫酸ナトリウムの連続滴定法で求められる。
【００１５】
本発明者らは、さらに検討を進めた結果、過カルボン酸と両性界面活性剤を成分とする薬剤及び過カルボン酸と鉱酸を成分とする薬剤がオーシストに対して優れた殺滅力を示すことを見いだした。
【００１６】
本発明で使用される両性界面活性剤は分子中にカチオンとアニオンを有する高分子であり、中でもアミノカルボン酸、イミダゾリニウムベタイン及びそれらの塩が好ましい。アミノカルボン酸としては、式（１）で示される脂肪族アミノエチルグリシンが特に好ましく、そのアルカリ金属塩等の水に可溶性の塩も好適に使用できる。イミダゾリニウムベタインとしては、式（２）で示される脂肪族イミダゾリニウムベタインが特に好ましく、そのアルカリ金属塩等の水に可溶性の塩も好適に使用できる。両性界面活性剤のうち、最も好ましいのは炭素数８〜１８のアルキル基を有するアルキル（アミノエチル）グリシンである。
【００１７】
【化１】
【００１８】
【化２】
【００１９】
また、鉱酸はその例として硫酸、硝酸及び塩酸が挙げられ、中でも塩酸が好ましい。
【００２０】
オーシストに対して殺滅効果を期待する場合、本発明の畜舎及び鶏舎用殺菌消毒剤は、様々な剤型で使用可能だが、好ましくは、成分濃度が以下のような水溶液が好適に使用される。すなわち、過カルボン酸の濃度は０．０４〜４０％（水溶液中の重量％；以下同じ）、好ましくは０．１０〜１０％である。過カルボン酸は水溶液中で過酸化水素及びカルボン酸と平衡状態にあるので、本発明の畜舎及び鶏舎用殺菌消毒剤は過カルボン酸の他に、過酸化水素及びカルボン酸を含み得る。
【００２１】
過酸化水素の濃度は０．０１〜３０％、好ましくは０．１０〜１０％である。カルボン酸の濃度は０．０５〜４０％、好ましくは０．１０〜３５％である。両性界面活性剤を使用する場合の添加量は０．００１〜４０％、好ましくは０．０１〜１０％である。鉱酸を使用する場合の添加量は０．００１〜４０％、好ましくは０．０１〜１０％である。
【００２２】
本発明の過カルボン酸を有効成分とする畜舎及び鶏舎用殺菌消毒剤は、鶏舎常在菌に対して市販のヨードホール剤と同等の殺菌効果を示す。また、本発明の過カルボン酸と両性界面活性剤の合剤又は過カルボン酸と鉱酸の合剤は、鶏舎常在菌に対する殺菌効果に加え、コクシジウム原虫胞子未形成オーシストに対しても優れた殺滅効果を示す。
【００２３】
さらに、過カルボン酸、又は、過カルボン酸と両性界面活性剤もしくは鉱酸を含有する水溶液からなる畜舎及び鶏舎用殺菌消毒剤は毒性が低いので、環境に対する適合性に優れている。
【００２４】
【実施例】
＜鶏舎常在菌に対する殺菌効果の試験＞
実施例１
ケージ飼育式の鶏舎のケージ下に３５ｃｍ×９ｃｍ、厚さ２ｍｍのベニヤ板を置き、落下して来る鶏糞で７２時間汚染させた。鶏糞で一様に汚染された板を軽く水洗して二分し、細菌の付着した板を試験板として２枚得た。一方、１ｍ×１ｍ×１ｍの大きさで前面が扉になっているベニヤ板製の箱を作成し、先に得た試験板の１枚を吊し、試験液として過酢酸２．２重量％、過酸化水素３．０重量％、酢酸１１．９重量％を含有する水溶液５００ｍｌを散布した後、扉を閉じ、２０〜２５℃で２４時間放置した。対照試験として、別の箱に試験板の残りの１枚を吊し、水５００ｍｌを散布した他は同様に放置した。
【００２５】
各試験板を取り出し、試験板表面の２ｃｍ×５ｃｍの部分を、生理食塩液で湿らせた滅菌済脱脂綿で拭い、細菌をそれぞれ採取した。
細菌を採取した脱脂綿を滅菌済生理食塩液１０ｍｌ中にいれて攪拌し、細菌浮遊液を得た。細菌浮遊液を希釈して、滅菌シャーレに注いだ。シャーレ中で細菌浮遊液と、加熱融解させたハートインフュジョン寒天培地を混釈させ、３７℃で２４時間培養し、生育したコロニーを数えて生残菌数を測定する為に菌数減少率を求めた。
【００２６】
以上の方法で試験液を散布した試験板と、対照試験板との間で生残菌数を比較し、試験液の鶏舎常在菌に対する有効性を判定した。
【００２７】
実施例２
試験液として過酢酸０．２２重量％、過酸化水素０．３重量％、酢酸１．１９重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００２８】
実施例３
試験液として過酢酸０．０６重量％、過酸化水素０．０８重量％、酢酸０．３２重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
実施例１〜３の鶏舎常在菌に対する殺菌効果の試験結果を第１表に示す。本発明の過酢酸水溶液は鶏舎常在菌に対して著しい殺菌作用を示した。
【００２９】
【表１】
実施例４
試験液として過酢酸０．１０重量％、過酸化水素０．１２重量％、酢酸０．５４重量％及び両性界面活性剤としてアミノカルボン酸塩の一種であるラウリルジ（アミノエチル）グリシンナトリウム０．２０重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００３１】
実施例５
試験液として過酢酸０．５０重量％、過酸化水素０．６７重量％、酢酸２．７０重量％及びラウリルジ（アミノエチル）グリシンナトリウム０．２０重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００３２】
実施例６
試験液として過酢酸１．００重量％、過酸化水素１．３重量％、酢酸５．３重量％及びラウリルジ（アミノエチル）グリシンナトリウム０．３重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００３３】
実施例４〜６の鶏舎常在菌に対する殺菌効果の試験結果を第１表に示す。本発明の過酢酸及び助剤を含有する水溶液は鶏舎常在菌に対して著しい殺菌作用を示した。
【００３４】
【表２】
＜鶏コクシジウムオーシストに対する殺滅効果の試験＞
実施例７
Ｅｉｍｅｒｉａ ｔｅｎｅｌｌａ家衛試株の胞子未形成オーシストを用いた。オーシストは、胞子形成オーシストを鶏に感染させ、感染７日後に糞便中に排出されたものをショ糖液浮遊法で回収して以下の試験に共通に使用した。
【００３６】
回収したオーシストを浮遊させた浮遊液０．５ｍｌ を過酢酸１．０重量％、過酸化水素１．３重量％、酢酸５．３重量％及びラウリルジ（アミノエチル）グリシンナトリウム０．２重量％を含有する水溶液６ｍｌに加え、２５℃で１時間感作させた。感作後、蒸留水で３回遠心洗浄し（２０００ｒｐｍ、５分）、試験液を洗浄除去した。洗浄後、オーシストを２％重クロム酸カリウム水溶液中で７日間培養した。培養後、１０００個のオーシストを鏡検し、形成された胞子の数を測定した。同様にして試験液に２５℃で２４時間感作させる操作を行い、胞子の数を測定した。
【００３７】
胞子形成率は、試験液の代わりに水を使用して同様に感作させたさせたものを対照にして、対照の胞子形成率を１００％として換算した。
【００３８】
実施例８
試験液として過酢酸０．５重量％、過酸化水素０．７重量％、酢酸２．７重量％及びラウリルジ（アミノエチル）グリシンナトリウム０．２重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００３９】
実施例９
試験液として過酢酸１．０重量％、過酸化水素１．４重量％、酢酸５．４重量％及びラウリルジ（アミノエチル）グリシンナトリウム０．２重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００４０】
実施例１０
試験液として過酢酸１．０重量％、過酸化水素１．３重量％、酢酸５．３重量％及びラウリルイミダゾリニウムベタイン０．２重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００４１】
実施例１１
試験液として過酢酸１．０重量％、過酸化水素１．３重量％、酢酸５．３重量％及び塩酸０．４重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００４２】
比較例１
試験液として過酢酸１．０重量％、過酸化水素１．３重量％、酢酸５．３重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００４３】
比較例２
試験液としてラウリルジ（アミノエチル）グリシンナトリウム０．２重量％を含有する水溶液を散布した他は実施例１と同様に測定した。
【００４４】
比較例３
試験液として、過酢酸及び非イオン性界面活性剤であるポリオキシエチレンアルキルエーテルを含有する水溶液を散布した他は実施例１と同様に測定した。
【００４５】
比較例４
試験液として、過酢酸及び非イオン性界面活性剤であるポリオキシエチレンアルキルフェニルエーテルを含有する水溶液を散布した他は実施例１と同様に測定した。
【００４６】
比較例５
試験液として、過酢酸及び非イオン性界面活性剤であるポリオキシエチレンソルビタンモノエステルを含有する水溶液を散布した他は実施例１と同様に測定した。
【００４７】
比較例６
試験液として、過酢酸及び非イオン性界面活性剤であるポリエチレングリコールモノラウレートを含有する水溶液を散布した他は実施例１と同様に測定した。
【００４８】
比較例７
試験液として、過酢酸と陰イオン性界面活性剤であるラウリルベンゼンスルホン酸ナトリウムを含有する水溶液を散布した他は実施例１と同様に測定した。
【００４９】
比較例８
試験液として、過酢酸と陽イオン性界面活性剤である塩化ドデシルトリメチルアンモニウムを含有する水溶液を散布した他は実施例１と同様に測定した。
【００５０】
比較例９
試験液として、過酢酸と陽イオン性界面活性剤である塩化ジデシルジメチルアンモニウムを含有する水溶液を散布した他は実施例１と同様に測定した。
【００５１】
比較例１０
試験液として、過酢酸と陽イオン性界面活性剤である塩化ラウリルピリジニウムを含有する水溶液を散布した他は実施例１と同様に測定した。
【００５２】
実施例８〜１１及び比較例１〜１０のオーシストに対する殺滅効果の試験結果を第３表に示す。
【００５３】
過酢酸又は助剤単独（比較例１〜２）に比べ、本発明の過酢酸と助剤の組合せ水溶液は少なくとも２４時間感作において著しいオーシストの殺滅作用を示した。比較例３〜１０で用いた助剤はいずれも、２４時間感作においてさえもオーシストの殺滅向上作用を示さなかった。
【００５４】
【表３】
【発明の効果】
本発明によれば、一般の細菌に対して十分な消毒殺菌効果を発揮する、安全、安定かつ安価な畜舎及び鶏舎用消毒殺菌剤が提供される。また、本発明によれば、オーシストに対して強い殺滅効果を発揮し、環境に対する悪影響が少ない、畜舎及び鶏舎用消毒殺菌剤が提供される。[0001]
[Industrial application fields]
The present invention relates to a disinfectant for barns and poultry houses.
[0002]
[Prior art]
Sterilization and sterilization of livestock houses and poultry houses is essential to prevent the spread and infection of livestock infectious diseases and to eliminate the microbial contamination of the breeding environment that accompanies breeding and to maintain the normal growth of livestock.
[0003]
Conventionally, for the disinfection and sterilization of barns and poultry houses, alcohol or aldehyde preparations, carboxylic acid or chlorophenol preparations, cresol preparations, inverse soap preparations, amphoteric soap preparations, halogen salt preparations, ortho-agents based on o-dichlorobenzene, etc. Drugs are being used.
[0004]
However, the above agents have drawbacks such as not become or have limited antibacterial spectrum, when sprayed on the actual house even if effective in culture solution, the reduction rate of the number of bacteria is only about 10 -1 (Kenji Furuta “Problems on disinfection in poultry facilities”, Journal of the Japanese Poultry Society, 30 , p. 325-335 (1993)). In addition, there are many drugs that are concerned about adverse effects on the environment, drainage, etc. around the application site. Therefore, there is a demand for a safe, stable and inexpensive sanitizer for poultry houses and poultry houses that exhibits a sufficient disinfecting and disinfecting effect.
[0005]
Of the diseases that are problematic in the field of livestock, the damage of chicken coccidiosis is large, but countermeasures against the diseases are difficult. Coccidiosis is caused by coccidiosis, a protozoan belonging to the genus Eimeria. Coccidium infects animals in the state of oocysts, becomes parasites within the animal body, and then grows parasitic on intestinal epithelial cells. Thereafter, the worm body becomes oocyst and is released out of the animal body together with feces. The oocysts immediately after being released to the outside of the animal body have no infectivity because they do not form spores, but after several days under suitable conditions, they form spores and exert infectivity.
[0006]
That is, coccidiosis is transmitted by oocysts, and if oocysts cannot be killed, the transmission of coccidiosis cannot be prevented. However, oocysts that are the source of infection have strong resistance to general disinfectants.
[0007]
As an antibacterial disinfectant effective against oocysts, ortho-agents based on o-dichlorobenzene are known. However, in the case of ortho-agents, bactericidal action is weak against bacteria other than oocysts, There are concerns about adverse effects on the environment around the spray site.
[0008]
For this reason, development of a safe, stable and inexpensive drug that has an oocyst killing effect and an excellent bactericidal effect against bacteria other than oocysts is desired.
[0009]
[Problems to be solved by the present invention]
The first object of the present invention is to provide a safe, stable and inexpensive disinfecting and disinfecting agent for barns and poultry houses that exhibits a sufficient disinfecting and disinfecting effect against general bacteria. The second object of the present invention is to provide a barn and poultry disinfectant having a strong killing effect against oocysts.
[0010]
[Means for Solving the Problems]
As a result of intensive studies in order to solve the above-mentioned problems, the present inventors have found that a drug containing percarboxylic acid as an active ingredient is effective as a disinfectant for barns and poultry houses.
[0011]
Examples of the percarboxylic acid used in the present invention include formic acid, peracetic acid, and perpropionic acid. Among them, peracetic acid is preferable. Percarboxylic acid has an excellent bactericidal effect, and since it decomposes relatively easily after spraying, it does not remain in the environment, and it has a low toxicity and thus has a small adverse effect on the environment.
[0012]
The disinfectant for livestock and poultry houses of the present invention can be used in various dosage forms, but is preferably used in the form of an aqueous solution.
[0013]
In the case of expecting a disinfecting and sterilizing effect on general bacteria (resident bacteria), aqueous solutions having the following component concentrations are preferably used. That is, the concentration of percarboxylic acid is 0.04 to 40% (% by weight in aqueous solution; the same applies hereinafter), preferably 0.10 to 10%. Since percarboxylic acid is in equilibrium with hydrogen peroxide and carboxylic acid in aqueous solution, the barn and poultry disinfectant of the present invention may contain hydrogen peroxide and carboxylic acid in addition to percarboxylic acid. The concentration of hydrogen peroxide is 0.01 to 30%, preferably 0.10 to 10%. The concentration of carboxylic acid is 0.05 to 40%, preferably 0.10 to 20%.
[0014]
The percarboxylic acid concentration was determined according to Yoshiro Ogata, “Chemistry of Organic Peroxides” Nanedo (1971) p. Reference is made to 331-343 to determine by a continuous titration method of potassium permanganate and sodium thiosulfate.
[0015]
As a result of further investigations, the present inventors have demonstrated that an agent containing a percarboxylic acid and an amphoteric surfactant and an agent containing a percarboxylic acid and a mineral acid have excellent killing power against oocysts. I found out.
[0016]
The amphoteric surfactant used in the present invention is a polymer having a cation and an anion in the molecule, and aminocarboxylic acid, imidazolinium betaine and salts thereof are particularly preferable. As the aminocarboxylic acid, aliphatic aminoethylglycine represented by the formula (1) is particularly preferable, and a water-soluble salt such as an alkali metal salt thereof can also be suitably used. As the imidazolinium betaine, an aliphatic imidazolinium betaine represented by the formula (2) is particularly preferable, and a water-soluble salt such as an alkali metal salt thereof can also be suitably used. Of the amphoteric surfactants, the most preferred is alkyl (aminoethyl) glycine having an alkyl group having 8 to 18 carbon atoms.
[0017]
[Chemical 1]
[0018]
[Chemical formula 2]
[0019]
Examples of the mineral acid include sulfuric acid, nitric acid and hydrochloric acid, with hydrochloric acid being preferred.
[0020]
In the case of expecting a killing effect on oocysts, the barn and poultry disinfectant of the present invention can be used in various dosage forms, but preferably an aqueous solution having a component concentration of the following is preferably used. . That is, the concentration of percarboxylic acid is 0.04 to 40% (% by weight in aqueous solution; the same applies hereinafter), preferably 0.10 to 10%. Since percarboxylic acid is in equilibrium with hydrogen peroxide and carboxylic acid in aqueous solution, the barn and poultry disinfectant of the present invention may contain hydrogen peroxide and carboxylic acid in addition to percarboxylic acid.
[0021]
The concentration of hydrogen peroxide is 0.01 to 30%, preferably 0.10 to 10%. The concentration of carboxylic acid is 0.05 to 40%, preferably 0.10 to 35%. When the amphoteric surfactant is used, the addition amount is 0.001 to 40%, preferably 0.01 to 10%. When using a mineral acid, the addition amount is 0.001 to 40%, preferably 0.01 to 10%.
[0022]
The barn and poultry disinfectant containing the percarboxylic acid of the present invention as an active ingredient exhibits a bactericidal effect equivalent to that of a commercially available iodophor agent against poultry house resident bacteria. Further, the combination of the percarboxylic acid and the amphoteric surfactant of the present invention or the combination of the percarboxylic acid and the mineral acid is superior to the coccidium protospore-free oocysts in addition to the bactericidal effect against the resident chickens. Shows killing effect.
[0023]
Furthermore, since the sterilizing and disinfecting agents for livestock and poultry houses composed of percarboxylic acid or an aqueous solution containing percarboxylic acid and an amphoteric surfactant or mineral acid have low toxicity, they are excellent in compatibility with the environment.
[0024]
【Example】
<Test of bactericidal effect against resident chickens>
Example 1
A veneer plate having a thickness of 35 cm × 9 cm and a thickness of 2 mm was placed under the cage of a cage-raising poultry house and contaminated with falling chicken droppings for 72 hours. The plate uniformly contaminated with chicken manure was lightly washed with water and divided into two, and two plates with bacteria attached were obtained as test plates. On the other hand, a box made of plywood with a size of 1 m × 1 m × 1 m and a front face serving as a door is created, one of the test plates obtained above is hung, and 2.2 wt% peracetic acid as a test solution, After spraying 500 ml of an aqueous solution containing 3.0% by weight of hydrogen peroxide and 11.9% by weight of acetic acid, the door was closed and allowed to stand at 20-25 ° C. for 24 hours. As a control test, the remaining one of the test plates was hung in another box and left in the same manner except that 500 ml of water was sprayed.
[0025]
Each test plate was taken out, and a 2 cm × 5 cm portion of the test plate surface was wiped with sterilized absorbent cotton moistened with physiological saline to collect bacteria.
Absorbent cotton from which bacteria were collected was placed in 10 ml of sterile physiological saline and stirred to obtain a bacterial suspension. The bacterial suspension was diluted and poured into a sterile petri dish. Bacteria suspension in a petri dish and heat-melted heart infusion agar medium were mixed, cultured at 37 ° C for 24 hours, and the number of grown colonies was counted. Asked.
[0026]
The number of surviving bacteria was compared between the test plate sprayed with the test solution by the above method and the control test plate, and the effectiveness of the test solution against the resident bacteria in the house was determined.
[0027]
Example 2
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 0.22% by weight of peracetic acid, 0.3% by weight of hydrogen peroxide and 1.19% by weight of acetic acid was sprayed as a test solution.
[0028]
Example 3
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 0.06% by weight of peracetic acid, 0.08% by weight of hydrogen peroxide and 0.32% by weight of acetic acid was sprayed as a test solution.
The test result of the bactericidal effect with respect to the poultry house resident bacteria of Examples 1 to 3 is shown in Table 1. The aqueous peracetic acid solution of the present invention showed a remarkable bactericidal action against resident chickens.
[0029]
[Table 1]
Example 4
As a test solution, 0.10% by weight of peracetic acid, 0.12% by weight of hydrogen peroxide, 0.54% by weight of acetic acid, and 0.20% sodium lauryl di (aminoethyl) glycine which is a kind of aminocarboxylate as an amphoteric surfactant. The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing wt% was sprayed.
[0031]
Example 5
Examples were sprayed with an aqueous solution containing 0.50% by weight of peracetic acid, 0.67% by weight of hydrogen peroxide, 2.70% by weight of acetic acid and 0.20% by weight of sodium lauryldi (aminoethyl) glycine as a test solution. Measurement was performed in the same manner as in 1.
[0032]
Example 6
Examples were sprayed with an aqueous solution containing 1.00% by weight of peracetic acid, 1.3% by weight of hydrogen peroxide, 5.3% by weight of acetic acid and 0.3% by weight of sodium lauryldi (aminoethyl) glycine as a test solution. Measurement was performed in the same manner as in 1.
[0033]
The test result of the bactericidal effect with respect to the resident house resident bacteria of Examples 4 to 6 is shown in Table 1. The aqueous solution containing the peracetic acid and auxiliary agent of the present invention showed a remarkable bactericidal action against the resident chickens.
[0034]
[Table 2]
<Test of killing effect against chicken coccidium oocysts>
Example 7
Non-spore-forming oocysts from Eimeria tenella Ie test strain were used. The oocysts were infected with spore-forming oocysts in chickens, and 7 days after infection, those excreted in the stool were collected by the sucrose suspension method and used in the following tests in common.
[0036]
0.5 ml of the suspended liquid in which the collected oocysts were suspended was mixed with 1.0% by weight of peracetic acid, 1.3% by weight of hydrogen peroxide, 5.3% by weight of acetic acid and 0.2% by weight of sodium lauryldi (aminoethyl) glycine. In addition to 6 ml of the aqueous solution contained, the mixture was sensitized at 25 ° C. for 1 hour. After sensitization, the test solution was washed and removed by centrifugal washing with distilled water three times (2000 rpm, 5 minutes). After washing, oocysts were cultured for 7 days in a 2% aqueous potassium dichromate solution. After culturing, 1000 oocysts were microscopically examined to determine the number of spores formed. Similarly, the test solution was sensitized at 25 ° C. for 24 hours, and the number of spores was measured.
[0037]
The spore formation rate was converted with the control spore formation rate as 100%, using a sensitized product that was similarly sensitized using water instead of the test solution.
[0038]
Example 8
Examples were sprayed with an aqueous solution containing 0.5% by weight of peracetic acid, 0.7% by weight of hydrogen peroxide, 2.7% by weight of acetic acid and 0.2% by weight of sodium lauryldi (aminoethyl) glycine as a test solution. Measurement was performed in the same manner as in 1.
[0039]
Example 9
Examples were sprayed with an aqueous solution containing 1.0% by weight of peracetic acid, 1.4% by weight of hydrogen peroxide, 5.4% by weight of acetic acid and 0.2% by weight of sodium lauryldi (aminoethyl) glycine as a test solution. Measurement was performed in the same manner as in 1.
[0040]
Example 10
Example 1 except that an aqueous solution containing 1.0% by weight of peracetic acid, 1.3% by weight of hydrogen peroxide, 5.3% by weight of acetic acid and 0.2% by weight of laurylimidazolinium betaine was sprayed as a test solution. It measured similarly.
[0041]
Example 11
Measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 1.0% by weight of peracetic acid, 1.3% by weight of hydrogen peroxide, 5.3% by weight of acetic acid and 0.4% by weight of hydrochloric acid was sprayed as a test solution. .
[0042]
Comparative Example 1
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 1.0% by weight of peracetic acid, 1.3% by weight of hydrogen peroxide, and 5.3% by weight of acetic acid was sprayed as a test solution.
[0043]
Comparative Example 2
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing 0.2% by weight of sodium lauryl di (aminoethyl) glycine was sprayed as a test solution.
[0044]
Comparative Example 3
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peroxyacetic acid and polyoxyethylene alkyl ether which is a nonionic surfactant was sprayed as a test solution.
[0045]
Comparative Example 4
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and polyoxyethylene alkylphenyl ether which is a nonionic surfactant was sprayed as a test solution.
[0046]
Comparative Example 5
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peroxyacetic acid and polyoxyethylene sorbitan monoester as a nonionic surfactant was sprayed as a test solution.
[0047]
Comparative Example 6
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and polyethylene glycol monolaurate which is a nonionic surfactant was sprayed as a test solution.
[0048]
Comparative Example 7
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and sodium laurylbenzenesulfonate as an anionic surfactant was sprayed as a test solution.
[0049]
Comparative Example 8
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and a cationic surfactant dodecyltrimethylammonium chloride was sprayed as a test solution.
[0050]
Comparative Example 9
The measurement was performed in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and cationic surfactant, didecyldimethylammonium chloride, was sprayed.
[0051]
Comparative Example 10
The measurement was carried out in the same manner as in Example 1 except that an aqueous solution containing peracetic acid and a cationic surfactant, laurylpyridinium chloride, was sprayed.
[0052]
The test results of the killing effect on the oocysts of Examples 8 to 11 and Comparative Examples 1 to 10 are shown in Table 3.
[0053]
Compared to peracetic acid or auxiliary agent alone (Comparative Examples 1-2), the combined aqueous solution of peracetic acid and auxiliary agent of the present invention showed a remarkable oocyst killing effect in sensitization for at least 24 hours. None of the auxiliaries used in Comparative Examples 3 to 10 showed an effect of improving the killing of oocysts even in 24-hour sensitization.
[0054]
[Table 3]
【The invention's effect】
ADVANTAGE OF THE INVENTION According to this invention, the safe, stable and cheap disinfection disinfectant for barns and poultry houses which exhibits sufficient disinfection disinfection effect with respect to general bacteria is provided. Moreover, according to this invention, the disinfection disinfectant for livestock houses and poultry houses which exhibits the strong killing effect with respect to an oocyst and there are few bad influences on an environment are provided.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP07165095A JP3624915B2 (en) | 1995-03-29 | 1995-03-29 | Disinfectant for barns and poultry houses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP07165095A JP3624915B2 (en) | 1995-03-29 | 1995-03-29 | Disinfectant for barns and poultry houses |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH08268807A JPH08268807A (en) | 1996-10-15 |
| JP3624915B2 true JP3624915B2 (en) | 2005-03-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP07165095A Expired - Fee Related JP3624915B2 (en) | 1995-03-29 | 1995-03-29 | Disinfectant for barns and poultry houses |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3624915B2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0211870B1 (en) | 2001-08-30 | 2016-01-12 | Embrex Inc | method for sporulation of viable eimeria oocysts |
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1995
- 1995-03-29 JP JP07165095A patent/JP3624915B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
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| JPH08268807A (en) | 1996-10-15 |
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